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Microscopes. Compound Bright-Field Light Microscope Maximum Useful Magnification = 1200X Why?. Resolution. Useful magnification is limited by Resolution Resolution is influenced by Numerical Aperture (NA) NA determined by Wavelength of Light and Refractive Index Improve Magnification - PowerPoint PPT Presentation
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Resolution
• Useful magnification is limited by Resolution• Resolution is influenced by Numerical Aperture
(NA)• NA determined by Wavelength of Light and
Refractive Index• Improve Magnification Shorten Wavelength Increase Refractive Index
Staining
• Simple Stains• Light Microscope -
Colored Dye• Electron Microscope - Electron Dense• Differential Stains Gram Stain
Gram Stain
Most Important Stain
Key Bacteriological Test
Conventional Stain
Multiple Steps & Subjective
Fluorescent Stain
One Step & +/-