MICROPIPETTES

Micropipettes

Step-by-Step to use Micropipettes1) Check the volume2) Attach disposable tip (different tips for each size micropipette)3) Depress the plunger (before you put in sample) to the first stop4) Immerse tip in sample5) Draw up the sample slowly and evenly, NOT all at once6) Pause7) Withdraw the tip8) Dispense the sample along the inside wall of tube to help coax out the sample9) Slowly depress plunger to second stop to expel last bit of fluid, and hold

plunger down in the position10) While holding plunger DOWN withdraw the pipette11) Release plunger12) Discard the tip*Always Change tips for each new reagent you need to pipette or if you touch

any other liquids. We want to remember our ASEPTIC techniques!

300µl (p1000 pipette)

120µl (p200 pipette)

50µl (p200 pipette)