MicroMax 384 v. 3.0 (9 Mar 2007) - Horiba · The MicroMax 384 is a microwell-plate reader able to accept plates with up to 384 wells. It connects to the FluoroMax® and Fluorolog®

MicroMax 384 v. 3.0 (9 Mar 2007)

MicroMax 384 v. 3.0 (9 Mar 2007)

i

MicroMax 384 with FluorEs-sence™

Operation Manual

Rev. 3 www.jobinyvon.com

MicroMax 384 v. 3.0 (9 Mar 2007)

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Copyright © HORIBA Jobin Yvon Inc. All rights reserved. No part of this work may be reproduced, stored, in a re-trieval system, or transmitted in any form by any means, including electronic or mechanical, photocopying and recording, without prior written permission from HORIBA Jobin Yvon Inc. Requests for permission should be requested in writ-ing. Origin® is a registered trademark of OriginLab Corporation. Windows® is a trademark of Microsoft Corporation. Information in this manual is subject to change without notice, and does not rep-resent a commitment on the part of the vendor. March 2007

Part Number 81056

MicroMax 384 v. 3.0 (9 Mar 2007)

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Table of Contents 1: Introduction ................................................................................................ 1-1

About the MicroMax..................................................................................................................................1-1 Disclaimer .................................................................................................................................................1-2 Safety summary ........................................................................................................................................1-4 Risks of ultraviolet exposure.....................................................................................................................1-6 Additional risks of xenon lamps ................................................................................................................1-8

2: Requirements & Installation ............................................................................ 2-1 Requirements............................................................................................................................................2-1 Installation.................................................................................................................................................2-2 Calibration and alignment of F-3000 or F4-3000 adapter.......................................................................2-13 Calibration and alignment of MicroMax 384 ...........................................................................................2-30

3: Operation of the MicroMax 384 ......................................................................... 3-1 Manual control of the MicroMax 384.........................................................................................................3-1 Running experiments with the MicroMax 384...........................................................................................3-7 Constant-wavelength analysis with the MicroMax 384...........................................................................3-14 Changing the type of microwell plate......................................................................................................3-22 Batch jobs ...............................................................................................................................................3-23

4: Daily Calibration Verification ........................................................................... 4-1 Introduction ...............................................................................................................................................4-1 Method ......................................................................................................................................................4-2

5: Tutorials.................................................................................................... 5-1 Introduction ...............................................................................................................................................5-1 Tutorial 1: Full-spectrum analysis .............................................................................................................5-2 Tutorial 2: Constant-wavelength analysis ...............................................................................................5-12

6: Maintenance............................................................................................... 6-1 Care of the fiber-optic cable......................................................................................................................6-1 Storage......................................................................................................................................................6-2

7: Troubleshooting ........................................................................................... 7-1 8: Technical Specifications................................................................................. 8-1

Computer requirements ............................................................................................................................8-1 Software requirements..............................................................................................................................8-1 Physical requirements...............................................................................................................................8-1 Electrical requirements .............................................................................................................................8-1 Fiber-optics’ requirements ........................................................................................................................8-1

9: Compliance Information ............................................................................. 9-1 Declaration of Conformity .........................................................................................................................9-1

10: Index..................................................................................................... 10-1

MicroMax 384 v. 3.0 (9 Mar 2007)

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MicroMax 384 v. 3.0 (9 Mar 2007) Introduction

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Note: Keep this and the other reference manuals near the system.

1: Introduction About the MicroMax 384

The MicroMax 384 is a microwell-plate reader able to accept plates with up to 384 wells. It connects to the FluoroMax® and Fluorolog® spectrofluorometers. MicroMax 384’s high speed allows it to scan completely a 96-well plate in less than one minute. By moving the microwell plate through stationary optics, the MicroMax 384 insures high sensitivity, excellent accuracy, and high reproducibility. Light from the excitation monochromator is carried via a fiber-optic bundle to the MicroMax 384. The fluores-cent response of the samples in the microwells is returned to the emission monochro-mator via the same fiber-optic bundle. The user may scan with the main spectrofluoro-meter, and select any excitation and emission wavelength-pair for intensity measure-ments. All control of the MicroMax 384 is automated through FluorEssence™ soft-ware. Custom selection of specific microwells on a plate is also controlled from the sofware.


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Disclaimer By setting up or starting to use any HORIBA Jobin Yvon product, you are accepting the following terms: You are responsible for understanding the information contained in this document. You should not rely on this information as absolute or all-encompassing; there may be local issues (in your environment) not addressed in this document that you may need to ad-dress, and there may be issues or procedures discussed that may not apply to your situa-tion. If you do not follow the instructions or procedures contained in this document, you are responsible for yourself and your actions and all resulting consequences. If you rely on the information contained in this document, you are responsible for: • Adhering to safety procedures • Following all precautions • Referring to additional safety documentation, such as Material Safety Data Sheets

(MSDS), when advised As a condition of purchase, you agree to use safe operating procedures in the use of all products supplied by HORIBA Jobin Yvon, including those specified in the MSDS provided with any chemicals and all warning and cautionary notices, and to use all safety devices and guards when operating equipment. You agree to indemnify and hold HORIBA Jobin Yvon harmless from any liability or obligation arising from your use or misuse of any such products, including, without limitation, to persons injured directly or indirectly in connection with your use or operation of the products. The foregoing indemnification shall in no event be deemed to have expanded HORIBA Jobin Yvon’s liability for the products. HORIBA Jobin Yvon products are not intended for any general cosmetic, drug, food, or household application, but may be used for analytical measurements or research in these fields. A condition of HORIBA Jobin Yvon’s acceptance of a purchase order is that only qualified individuals, trained and familiar with procedures suitable for the products ordered, will handle them. Training and maintenance procedures may be pur-chased from HORIBA Jobin Yvon at an additional cost. HORIBA Jobin Yvon cannot be held responsible for actions your employer or contractor may take without proper training. Due to HORIBA Jobin Yvon’s efforts to continuously improve our products, all speci-fications, dimensions, internal workings, and operating procedures are subject to change without notice. All specifications and measurements are approximate, based on a standard configuration; results may vary with the application and environment. Any software manufactured by HORIBA Jobin Yvon is also under constant development and subject to change without notice. Any warranties and remedies with respect to our products are limited to those provided in writing as to a particular product. In no event shall HORIBA Jobin Yvon be held li-


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able for any special, incidental, indirect or consequential damages of any kind, or any damages whatsoever resulting from loss of use, loss of data, or loss of profits, arising out of or in connection with our products or the use or possession thereof. HORIBA Jo-bin Yvon is also in no event liable for damages on any theory of liability arising out of, or in connection with, the use or performance of our hardware or software, regardless of whether you have been advised of the possibility of damage.


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Safety summary The following general safety precautions must be observed during all phases of opera-tion of this instrument. Failure to comply with these precautions or with specific warn-ings elsewhere in this manual violates safety standards of design, manufacture and in-tended use of instrument. HORIBA Jobin Yvon assumes no liability for the customer’s failure to comply with these requirements. Certain symbols are used throughout the text for special conditions when operating the instruments:

A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or similar that, if incorrectly performed or ad-hered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met. HORIBA Jobin Yvon Inc. is not responsible for damage arising out of improper use of the equipment.

A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or similar that, if incorrectly performed or ad-hered to, could result in damage to the product. Do not proceed beyond a CAUTION notice until the indicated conditions are fully under-stood and met. HORIBA Jobin Yvon Inc. is not responsible for damage arising out of improper use of the equipment.

Ultraviolet light! Wear protective goggles, full-face shield, skin-protection clothing, and UV-blocking gloves. Do not stare into light.

Intense ultraviolet, visible, or infrared light! Wear light-protective goggles, full-face shield, skin-protection clothing, and light-blocking gloves. Do not stare into light.

Danger to fingers! This symbol warns the user that the equipment is heavy, and can crush or injure the hand if precautions are not taken. Warning:

Caution:

Caution:

Caution:

Warning:


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This symbol cautions the user that excessive humidity, if present, can damage certain equipment.

Read this manual before using or servicing the instrument.

Wear protective gloves.

Wear appropriate safety goggles to protect the eyes.

Wear an appropriate face-shield to protect the face.

General information is given concerning opera-tion of the equipment.

Note:

Caution:


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Risks of ultraviolet exposure Do not aim the UV light at anyone. Do not look directly into the light. Always wear protective goggles, full-face shield and skin protection clothing and gloves when using the light source. • Light is subdivided into visible light, ranging from 400 nm (violet) to 700 nm (red);

longer infrared, “above red” or > 700nm, also called heat; and shorter ultraviolet ra-diation (UVR), “below violet” or < 400nm. UVR is further subdivided into UV-A or near-UV (320–400 nm), also called black (invisible) light; UV-B or mid-UV (290–320 nm), which is more skin penetrating; and UV-C or far-UV (< 290 nm).

• Health effects of exposure to UV light are familiar to anyone who has had sunburn.

However, the UV light level around some UV equipment greatly exceeds the level found in nature. Acute (short-term) effects include redness or ulceration of the skin. At high levels of exposure, these burns can be serious. For chronic exposures, there is also a cumulative risk of harm. This risk depends upon the amount of exposure during your lifetime. The long-term risks for large cumulative exposure include premature aging of the skin, wrinkles and, most seriously, skin cancer and cataract.

• Damage to vision is likely following exposure to high-intensity UV radiation. In

adults, more than 99% of UV radiation is absorbed by the anterior structures of the eye. UVR can contribute to the development of age-related cataract, pterygium, photodermatitis, and cancer of the skin around the eye. It may also contribute to age-related macular degeneration. Like the skin, the covering of the eye or the cor-nea, is epithelial tissue. The danger to the eye is enhanced by the fact that light can enter from all angles around the eye and not only in the direction of vision. This is especially true while working in a dark environment, as the pupil is wide open. The lens can also be damaged, but because the cornea acts as a filter, the chances are re-

Caution: This instrument is used in conjunction with ul-traviolet light. Exposure to these radiations, even re-flected or diffused, can result in serious, and sometimes irreversible, eye and skin injuries.

Overexposure to ultraviolet rays threatens human health by causing:

• Immediate painful sunburn • Skin cancer • Eye damage • Immune-system suppression • Premature aging


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duced. This should not lessen the concern over lens damage however, because cata-racts are the direct result of lens damage.

Burns to the eyes are usually more painful and serious than a burn to the skin. Make sure your eye protection is appropriate for this work. NORMAL EYEGLASSES OR CONTACTS OFFER VERY LIMITED PROTECTION!

Training For the use of UV sources, new users must be trained by another member of the labora-tory who, in the opinion of the member of staff in charge of the department, is suffi-ciently competent to give instruction on the correct procedure. Newly trained users should be overseen for some time by a competent person.

Warning: UV exposures are not immediately felt. The user may not realize the hazard until it is too late and the damage is done.


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Additional risks of xenon lamps

Among the dangers associated with xenon lamps are: • Burns caused by contact with a hot xenon lamp. • Fire ignited by hot xenon lamp. • Interaction of other nearby chemicals with intense ultraviolet, visible, or infrared

radiation. • Damage caused to apparatus placed close to the xenon lamp. • Explosion or mechanical failure of the xenon lamp.

Visible radiation Any very bright visible light source will cause a human aversion response: we either blink or turn our head away. Although we may see a retinal afterimage (which can last for several minutes), the aversion response time (about 0.25 seconds) normally protects our vision. This aversion response should be trusted and obeyed. NEVER STARE AT ANY BRIGHT LIGHT-SOURCE FOR AN EXTENDED PERIOD. Overriding the aversion response by forcing yourself to look at a bright light-source may result in per-manent injury to the retina. This type of injury can occur during a single prolonged ex-posure. Excessive exposure to visible light can result in skin and eye damage. Visible light sources that are not bright enough to cause retinal burns are not necessar-ily safe to view for an extended period. In fact, any sufficiently bright visible light source viewed for an extended period will eventually cause degradation of both night and color vision. Appropriate protective filters are needed for any light source that causes viewing discomfort when viewed for an extended period of time. For these rea-sons, prolonged viewing of bright light sources should be limited by the use of appro-priate filters. The blue-light wavelengths (400–500 nm) present a unique hazard to the retina by caus-ing photochemical effects similar to those found in UV-radiation exposure.

Infrared radiation Infrared (or heat) radiation is defined as having a wavelength between 780 nm and 1 mm. Specific biological effectiveness “bands” have been defined by the CIE (Commis-sion International de l’Eclairage or International Commission on Illumination) as fol-lows: • IR-A (near IR) (780–1400 nm) • IR-B (mid IR) (1400– 3000 nm) • IR-C (far IR) (3000 nm–1 mm)

Warning: Xenon lamps are dangerous. Please read the fol-lowing precautions.


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The skin and eyes absorb infrared radiation (IR) as heat. Workers normally notice ex-cessive exposure through heat sensation and pain. Infrared radiation in the IR-A that enters the human eye will reach (and can be focused upon) the sensitive cells of the ret-ina. For high irradiance sources in the IR-A, the retina is the part of the eye that is at risk. For sources in the IR-B and IR-C, both the skin and the cornea may be at risk from “flash burns.” In addition, the heat deposited in the cornea may be conducted to the lens of the eye. This heating of the lens is believed to be the cause of so called “glass-blowers’ ” cataracts because the heat transfer may cause clouding of the lens. • Retinal IR Hazards (780 to 1400 nm): possible retinal lesions from acute high ir-

radiance exposures to small dimension sources. • Lens IR Hazards (1400 to 1900 nm): possible cataract induction from chronic lower

irradiance exposures. • Corneal IR Hazards (1900 nm to 1 mm): possible flashburns from acute high irradi-

ance exposures. Who is likely to be injured? The user and anyone exposed to the radiation or xenon lamp shards as a result of faulty procedures. Injuries may be slight to severe.


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MicroMax 384 v. 3.0 (9 Mar 2007) Requirements & Installation

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2: Requirements & Installation Requirements

Physical requirements • Located within 0.75 m of the spectrofluorometer sample compartments • Needs 10″ (25 cm) height minimum including the MicroMax 384, to account for the

fiber-optic bundle. • Requires F-3000 (Fluorolog®-3) or F4-3000 (FluoroMax®-4) fiber-optic adapter ac-

cessory

Computer requirements • Dedicated serial COM port • FluorEssence™ software version 2.1 • Windows® XP Pro software • 973005 or equivalent PC as host computer


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Note: Many public carriers will not recognize a claim for concealed damage if it is reported later than 15 days af-ter delivery. In case of a claim, inspection by an agent of the carrier is required. For this reason, the original pack-ing material should be retained as evidence of alleged mishandling or abuse. While HORIBA Jobin Yvon Inc. assumes no responsibility for damage occurring during transit, the company will make every effort to aid and advise.

Caution: The MicroMax 384 is a delicate instrument. Mishandling may seriously damage its components.

Warning: Watch your fingers!

Installation 1 Unpack and inspect

all components a The following MicroMax 384 components should be included:

Quantity Description Part Number

1 MicroMax 384 microwell plate reader 1 MicroMax 384 with FluorEssence™ Operation

Manual 81051

1 AC (mains) power cable 98015 1 RS-232 communications cable 97133 1 RS-232 female-9-pin-to-male-25-pin adapter 97134 1 Bifurcated fiber-optic cable, 1 m long 650025 1 Lens assembly for fiber-optic cable 650026 1 Fiber-optic interface for spectrofluorometer

(sold separately from MicroMax 384) F-3000 (for Fluorolog®) or F4-3000 (for FluoroMax®-4)

b Inspect all components for signs of damage that may have occurred during transit. If damage is evident, do not continue with the installation. Instead, notify HORIBA Jobin Yvon Inc. and the shipper at once.


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Caution: Operating the MicroMax 384 with-out removing the white plastic rectangular plate can damage the MicroMax 384!

2 Read this instruction manual thor-oughly before proceeding to install the MicroMax 384.

3 Install the F-3000 or F4-3000 fiber-optic adapter on the spectrofluorometer. a Be sure that the MicroMax 384, host computer, and spectrofluorometer

are all switched off.

b Open the lid of the MicroMax 384, and remove the white plastic rectangular plate. This plate was used to prevent the base from moving during shipping.

c Remove the existing sample mount from the spectrofluorometer’s sample chamber. Save the two brass thumbscrews for mounting the fiber-optic adapter.

d Insert the F-3000 or F4-3000 fiber-optic adapter into the spectrofluorometer’s sample compartment. One mirror should face the excitation monochromator, and the other should face the emission monochromator.

e Secure the baseplate by using two brass thumbscrews. The lens and baffle should be closer to the excitation monochromator.

For Fluorolog®-3 users:

f Remove the 1⅜″ (3.5 cm) diameter plastic plug from the front of the spectrofluorometer’s sample compartment. If there are any threaded plugs for set screws in the two adjacent mounting holes, remove them, too.

g Remove the plastic shipping caps from the fiber-optic cable’s ferrules.


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Gasket (underneath light-shield plate) Light-shield plate

h Thread the two slit-ended fiber bundles through the hole in the sample compartment.

i Insert the fiber bundle labeled “excitation fibers” into the excitation port,

and then insert the other (unlabeled) fiber bundle into the other port, as shown below:

j Secure the fiber-optic bundles loosely with the two narrow thumbscrews on the F-3000 or F4-3000 adapter, in their respective mounts.

k Mount the plate and gasket on the outside of the sample compartment, as shown in this photograph:

Caution: Avoid twisting the fiber-optic bundles too much. Breakage of the glass fibers may occur with excessive strain.


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For FluoroMax®-4 users:

f With a 9/64″ Allen key, remove the 4 inside cap screws from the front wall of the sample compartment. The existing blue plate detaches from the sam-ple mount.

g Remove the 1⅜″ (3.5 cm) diameter plastic plug from the new blue plate. Insert the rectangular gasket into the rectangular depression.

h Attach the new blue plate with gasket to the sample mount, using the 4 cap screws. Thread the two slit-ended fiber bundles through the hole in the sample compartment.

i Insert the fiber bundle labeled “excitation fibers” into the excitation port,

and then insert the other (unlabeled) fiber bundle into the other port, as shown below:


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Note: Which COM port chosen depends on the settings in the instrument configuration, described later.

j Secure the fiber-optic bundles loosely with the two narrow thumbscrews on the F-3000 or F4-3000 adapter, in their respective mounts.

4 Assemble the MicroMax 384 and accessories. a Place the MicroMax 384 on a level surface near the spectrofluorometer’s

sample compartment, so that the fiber-optic bundles reach both sample and MicroMax 384.

b Verify that the MicroMax 384 is set for the proper line voltage. The power (mains) receptacle has a white arrow that indicates the AC voltage set by the factory.

c Connect the AC power cord (mains) to the receptacle on the MicroMax 384.

d Attach one end of the RS-232 communication cable to the I/O connector on the MicroMax 384.

e Attach the other end of the RS-232 cable to the free COM port on the back of the host computer. Use the enclosed 9-pin–25-pin adapter as necessary.

Caution: Avoid twisting the fiber-optic bundles too much. Breakage of the glass fibers may occur with excessive strain.


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f Insert the combined end of the fiber-optic cable into the lens cell. The cable should reach the bottom of the cell.

g Secure the cable to the lens by tightening the set screw on the lens cell.

h Insert the lens-cell assembly into the receptacle at the top of the MicroMax 384, so that it is firmly sealed. The top of the lens should protrude about ⅛″ (~2 mm) from the recepta-cle.

i Tighten the nylon thumbscrew on the lens receptacle. This secures the lens-cell assembly in place.

j Plug the free end of the power (mains) cord into a properly rated AC receptacle.

Initial positioning for alignment is complete.

~ 2 mm

Caution: Never pull the calbe itself—the ferrule may loosen from the cable. Hold the cable by the metal ferrules only..


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5 Create a new instrument configuration. (only if the MicroMax 384 was purchased separately from the spectrofluorome-ter, otherwise skip to step 6)

a Double-click the FluorEssence icon on the desktop to start the FluorEssence™ software.

The main FluorEssence window appears.

b Choose the Collect menu. A drop-down menu appears.

c Choose Experiment Setup. The Select Hardware Configuration window appears.

Note: If you plan to use plates with different numbers of mi-crowells (e.g., 384 or 96), create a separate instrument configu-ration for each type of plate.


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d Choose the New button. The System Configuration Wizard window appears.

e Choose the Standard Instrument Configuration radio button, and click Next >>. The System Selection Page window ap-pears.

f Choose the main instrument to which the MicroMax 384 is to be connected, and its model (as necessary) from the drop-down menu, then click Next >.


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The Configu-ration Name window ap-pears.

g Enter a distinguishing name for this instrument configuration, and click Next >. The Fluoro-max Configu-ration window appears.

h Activate the MicroMax checkbox, and click Next >. The MicroMax Communica-tion Settings window ap-pears.

i Choose a free COM port, and click the OK button. A Dialog win-dow appears.


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j From the drop-down menu, choose the type of plate, and click Add >>. The plate ap-pears in the empty box on the right.

k On the left side of the accessory, read the offset values and enter them into the X Origin and Y Origin fields, then click the OK button.

The Summary window appears. This window is not editable, but shows the in-strument con-figuration to be activated.

l If this configuration is correct, click the Finish button.

m To use another type of microwell plate, repeat step 5.

6 Calibrate and align the F-3000 or F4-3000 adap-ter. This procedure is explained in the next section of this chapter.

Note: Even if the offset shows nega-tive values, en-ter positive val-ues.


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7 Calibrate and align the MicroMax 384. This procedure is explained in the section after calibrating the F-3000 or F4-3000 adapter.


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Calibration and alignment of F-3000 or F4-3000 adapter

1 Start the MicroMax 384 and Fluo-rEssence™ software. a Be sure that FluorEssence™ is installed on the

host computer.

b Start the spectrofluorometer.

c Turn on the MicroMax 384 via its power switch.

d Double-click the FluorEssence icon on the desktop to start the FluorEssence™ software.

The main FluorEssence window appears.

e Click the Experiment Menu button . The Select Hardware Configuration window appears.


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f Choose the appropriate instrument configuration with a MicroMax 384 attached.

The FluorEssence Main Experiment Menu opens.

g Choose the type of experiment to run.

The Experiment Type window asks what subtype of experiment to set up.

h Choose the desired subtype, and click Next >>. The Experiment Setup window ap-pears.

Note: Grayed-out buttons are not available on your instrument con-figuration.

Note: If you have only one instru-ment configura-tion, this window does not appear.


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2 Activate the MicroMax 384. a Click the Accessories icon.

FluorEssence™ considers the MicroMax 384 an accessory.

The accessories’ tabs appear:

Note: The number of tabs varies depending on the ac-cessories set up in your instrument’s configuration.


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b Choose the MicroMax tab. The grayed-out parameters of the MicroMax tab appear:


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c Pick a microwell by clicking on a gray circle on the plate schematic. Any microwell may be chosen; this is necessary for the MicroMax 384 to appear in the Real Time Control.

d Click the Activate checkbox to activate the MicroMax 384. Several items in the MicroMax tab become active.


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e Click the RTC button to enter the Real Time Control window.


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f Click the Accessories icon. FluorEssence™ considers the MicroMax 384 an accessory.

3 Examine the alignment. a Set the excitation monochromator to 500 nm (blue-green light) by

entering 500.

b Set the Band Pass to 5 nm.

c Open the shutter.

d Remove the top of the spectrofluorometer’s sample compartment.

Caution: Intense ultraviolet, visible, or infra-red light may be present when the sample compartment is open. Do not aim fiber-optic bundles onto the skin or eyes. Use extreme caution with the fiber-optic probes.


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e With a dental mirror, check to see that the bright blue-green, slit-shaped beam falls properly on the slit-shaped entrance to the excitation fibers. The beam should fall directly on the fibers:

f If the beam is not in the center, remove the fiber-optic cables and the F-3000 or F4-3000 adapter, and insert an Allen wrench in the central hole between the mounting screws. Loosen the front-mirror set screw, and adjust the mirror’s left-right posi-tion slightly.

Loosen the interior-mirror set screw, and adjust the mirror’s back posi-tion slightly. Replace the F-3000 or F4-3000 and fiber-optics in the sample compart-ment, and examine the beam again.

g Repeat step f until the beam falls properly on the entrance of the fiber bundle.

h Reduce the band pass to 2 nm, and examine the beam again. The beam should fall directly on the fibers. If not, repeat step f.

i Reduce the band pass to 1 nm, and re-examine the beam. The beam should fall directly on the fibers. If not, repeat step f.

Slit-shaped bundle of fibers

Beam of light

End of ferrule



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j Remove the F-3000, rotate it 90° counterclockwise,

and replace it in the sample compartment so that the emission beam now is at the excitation-fiber position.

k With the dental mirror, examine the slit-shaped beam as in steps e through i.

Verify that the light falls in the middle of the emission’s ferrule end. Adjust the mirror as needed. (A later pro-cedure will tune the emission’s signal level.)

l Remove the F-3000 or F4-3000 adapter, rotate it 90° clockwise back to its original position, and fasten it down with the two thumbscrews.

When the MicroMax is turned on and connected to the host computer, the microwell-plate holder automatically moves to the Home position.

4 Calibrate the MicroMax 384 with standard and blank.

Note: The following steps assume that fluorescein is the sample used to calibrate and align. Other fluorescent solutions may re-quire different settings.

Note: If the F-3000 or F4-3000 adapter was purchased with the instrument, the mirrors are now aligned. If the F-3000 or F4-3000 was purchased separately, continue aligning the mirrors below.



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a Insert a clean, unused microwell plate containing: • A microwell with 200 µL of 100 nM fluorescein (extinction coeffi-

cient ~ 80 000) in 0.1 N NaOH—the standard. • A microwell with 200 µL of 0.1 N NaOH—the blank solution. The fluorescein gives an absorbance A = 0.008 at 490 nm above the blank.

b Close the sample-compartment lid.

c Start the FluorEssence™ software, and load an instrument configuration with the MicroMax 384. At the end of the initialization process, the MicroMax 384 makes a char-acteristic sound as it resets to the home position.


d Choose a type of experiment to run.

If necessary, the Ex-periment Type window asks what subtype of experiment to set up.

e Choose the desired subtype, and click Next >>. The Experiment Setup window ap-pears.


Note: Be sure to use fluorescein in basic solution. Acidified solu-tions of fluorescein have a low quantum yield, so the spectro-fluorometer’s sensitivity will be far lower than expected.


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f Click the Accessories icon. FluorEssence™ considers the MicroMax 384 an accessory.




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g Pick a microwell by clicking on any gray circle in the plate schematic. This is necessary to activate the MicroMax 384 in the Real Time Control.

h Click the Activate checkbox to activate the MicroMax 384. Several items in the MicroMax tab become active.


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i Click the RTC button to enter the Real Time Control window.


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j Click the Accessories icon. FluorEssence™ considers the MicroMax 384 an accessory.

k Click the MicroMax tab.


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l On the sketch of the microwell plate, click the position of the standard in the microwell plate. The value, e.g., A12, should appear in the Current Cell field, and the microwell plate should move to this position.

m Click the General icon.


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n In the Excitation 1 area, set the excitation monochromator’s Position to 485 nm by typing in 485 in Position, and the Slits to 2 nm.

o In the Emission 1 area, set the emission monochromator’s Position to 513 nm by typing in 513 in Position and the Slits to 2 nm.

p Move the Shutter Mode switch to Open. This opens the excitation shutter.

q Click the Run button.

r Watch the S signal intensity in the black graph or the Signal Intensity tab. If, at any time during alignment, the signal > 4 × 106 cps, reduce the Slits settings.


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s Open the sample-compartment cover.

t Adjust the front set screw for the emission mirror to maximize the signal intensity. This controls the “pitch” (front-back fine rotation) of the mirror.

u Adjust the side set screw for the emission mirror to maximize the signal intensity. This controls the left-right fine rotation of the mirror.

The mirrors should now be aligned.

Note: Do not adjust the signal for the excitation mirror. If you have trouble adjusting the position of the light beam on the ferrules, or maximizing the signal, contact Spex®

Fluorescence Service.

Caution: Intense ultraviolet, visible, or in-frared light may be present when the sample compartment is open. Do not aim fiber-optic bundles onto the skin or eyes. Use extreme caution with the fi-ber-optic probes.


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Calibration and alignment of MicroMax 384 1 Be sure that FluorEssence™ is properly installed

on the host computer. 2 Start the spectrofluorometer. 3 Set up the MicroMax 384.

a Insert a clean, unused microwell plate containing: • A microwell with 200 µL of 100 nM fluorescein (extinction coeffi-

cient ~ 80 000) in 0.1 N NaOH—the standard. • A microwell with 200 µL of 0.1 N NaOH—the blank solution. The fluorescein gives an absorbance A = 0.008 at 490 nm above the blank.

b Close the sample-compartment lid.

c Start the FluorEssence™ software, and load an instrument configuration with the MicroMax 384. At the end of the initialization process, the MicroMax 384 makes a char-acteristic sound as it resets to the home position.


d Choose a type of experiment to run.


Note: Be sure to use fluorescein in basic solution. Acidified solu-tions of fluorescein have a low quantum yield, so the spectro-fluorometer’s sensitivity will be far lower than expected.


2-31

If necessary, the Experiment Type window asks what subtype of ex-periment to set up.

e Choose the desired subtype, and click Next >>. The Experiment Setup window ap-pears.

f Click the Accessories icon. FluorEssence™ considers the Mi-croMax 384 an accessory.




2-32

g Click the Activate checkbox to activate the MicroMax 384. Several items in the MicroMax tab become active.


2-33

h Pick a microwell by clicking on any gray circle in the plate schematic. This is necessary to activate the MicroMax 384 in the Real Time Control.

i Click the RTC button to enter the Real Time Control window.


2-34

j Click the Accessories icon. FluorEssence™ considers the MicroMax 384 an accessory.

k Click the MicroMax tab.


2-35

l On the sketch of the microwell plate, click the position of the standard in the microwell plate. The value, e.g., A12, should appear in the Current Cell field, and the microwell plate should move to this position.

m Click the General icon.


2-36

n In the Excitation 1 area, set the excitation monochromator’s Position to 485 nm by typing in 485 here, and the Slits to 2 nm.

o In the Emission 1 area, set the emission monochromator’s Position to 513 nm by typing in 513 here and the Slits to 2 nm.

p Move the Shutter Mode switch to Open. This opens the excitation shutter.

q Watch the S signal intensity in the black graph or the Signal Intensity tab. If, at any time during alignment, the signal > 4 × 106 cps, reduce the Slits settings.


2-37

4 Align the Mi-croMax 384. a Loosen the nylon

thumbscrew on the fiber-optic adapter assembly.

b Gently adjust the height of the fiber-optic assembly above the microwell plate, in order to maximize the S signal.

c With maximum signal, tighten the nylon thumbscrew. The MicroMax 384 is now properly aligned for this sample at its current vol-ume. The procedure need not be repeated unless the sample’s volume changes.

Note: The background count of a microwell plate is large, there-fore we recommend that you subtract a blank scan from the sample scan to give ultimate sensitivity. See the on-line help and the User’s Guide to FluorEssence™ for more information on blank-subtraction.

Note: This alignment of the fiber-optic assembly should be per-formed each time a different sample volume is used.


2-38

MicroMax 384 v. 3.0 (9 Mar 2007) Operation of the MicroMax 384

3-1

3: Operation of the MicroMax 384 Manual control of the MicroMax 384

Manual control of the MicroMax 384 is found in the Real Time Control, where you can move the microwell plate to particular wells and see the resulting fluorescence in real time.

1 Start the FluorEssence™ software, and load an instrument configuration with the MicroMax 384. At the end of the initialization process, the MicroMax 384 makes a characteris-tic sound as it resets to the home position. The FluorEs-sence Main Experiment Menu opens.

2 Choose a type of experiment to run.

If necessary, the Experiment Type window asks what subtype of experiment to set up.

3 Choose the desired sub-type, and click Next >>. The Experiment Setup window appears.


The MicroMax 384 cannot be used with the AutoTitrator or temperature-bath accessories.


3-2

Note: The number of tabs varies depending on the accessories set up in your instrument’s configuration.

4 Click the Accessories icon. FluorEssence™ considers the MicroMax 384 an accessory.



3-3

d Pick a microwell by clicking on any gray circle in the plate schematic. This is necessary to activate the MicroMax 384 in the Real Time Control.

5 Click the Activate checkbox to activate the Mi-croMax 384. Several items in the MicroMax tab become active.


3-4

6 Click the RTC button to enter the Real Time Control window.


3-5

7 Click the Accessories icon. FluorEssence™ considers the MicroMax 384 an accessory.

8 Click the MicroMax tab.


3-6

9 On the sketch of the microwell plate, click the po-sition of the standard in the microwell plate. The value, e.g., A12, should appear in the Current Cell field, and the microwell plate should move to this position.

10 To return to the home position, click the Home button.


3-7

Running experiments with the MicroMax 384

Introduction The Experiment Setup window provides all scan-ning capability for the spectrofluorometer, with or without the MicroMax 384. The 3D scan-type is not possible with the MicroMax 384.

Method

1 In the main FluorEssence window, click the Ex-periment Menu button.

Note: The MicroMax 384 cannot be used with the AutoTitrator or temperature-bath accessories.


3-8

The Select Hardware Configuration window appears.

2 Choose the appro-priate instrument configuration with a MicroMax 384 at-tached. The FluorEssence Main Ex-periment Menu opens.

3 Choose the type of experiment to run.


4 As necessary, choose the desired subtype, and click Next >>. The Experiment Setup window appears.


Note: If you have only one instrument con-figuration, this win-dow does not appear.


3-9


FluorEssence™ considers the MicroMax 384 an accessory.




3-10

b Choose the MicroMax tab. The grayed-out parameters of the MicroMax tab appear:


3-11

c Click the Activate checkbox to activate the MicroMax 384. Several items in the MicroMax tab become active.


3-12

d Choose the radio button for the type of sample in each well: Radio button Color code on

diagram Type of sample

Empty radio button

gray No sample is in the well, or un-scanned

Blank radio button

blue Blank sample

Standard radio button

green Standard sample

Unknown radio button

red Unknown sample to be determined by curve fitting or other means

• To choose a microwell, click on a corner of the diagram’s area to be

denoted as a particular sample type and drag through the area, or click on each individual microwell.

• To select all microwells, click the Select All button. • To clear all microwells, click the Clear All button. As microwells are designated, their position and type appear in the table on the right side of the window.


3-13

• To delete a row from the table, click in the undesired row(s), and then click the Delete Row(s) button.

Below is a sample window with many microwells designated for scan-ning:

e The radio buttons in the Curve Fitting area are inactive in the current version of FluorEssence™.

f Double-click the Concentration field in each Standard’s row, type its concentration, then hit the Enter key.

6 Click the Run button to run the experiment.


3-14

Constant-wavelength analysis with the Mi-croMax 384

Introduction Constant-wavelength analysis, or the Single Point scan-type, allows acquisition of ex-citation and emission pairs. Results may be plotted or retained as a table. Up to 32 wavelength-pairs and multiple acquisition modes may be selected. Sample positions may be tagged as blanks, standards, or unknowns. Data manipulation and correction in-cludes: • Blank subtraction • Concentration curves • Correction files for inhomogeneities in lamps and spec-

trofluorometers • Variable-time kinetics, i.e., acquire scans at different

time-intervals, and use different integration times • Photobleach mode, i.e., protect light-sensitive samples

by closing the shutter during dead time

Method

1 In the main FluorEssence window, click the Ex-periment Menu button.

Note: The Mi-croMax 384 cannot be used with the AutoTitrator or tempera-ture-bath ac-cessories.


3-15



3 Choose Single Point.

The Experiment Setup window appears.

4 Activate the MicroMax 384.




3-16

g Click the Accessories icon. FluorEssence™ considers the MicroMax 384 an accessory. The accessories’ tabs appear:



3-17

h Choose the MicroMax tab. The grayed-out parameters of the MicroMax tab appear:


3-18

i Click the Activate checkbox to activate the MicroMax 384. Several items in the MicroMax tab become active.


3-19

j Choose the radio button for the type of sample in each well: Radio button Color code on

diagram Type of sample

Empty radio button

gray No sample is in the well, or un-scanned

Blank radio button

blue Blank sample

Standard radio button

green Standard sample

Unknown radio button

red Unknown sample to be determined by curve fitting or other means

• To choose a microwell, click on a corner of the diagram’s area to be

denoted as a particular sample type and drag through the area, or click on each individual microwell.

• To select all microwells, click the Select All button. • To clear all microwells, click the Clear All button. As microwells are designated, their position and type appear in the table on the right side of the window.


3-20

• To delete a row from the table, click in the undesired row(s), and then click the Delete Row(s) button.

Below is a sample window with many microwells designated for scan-ning:

k The radio buttons in the Curve Fitting area are inactive in the current version of FluorEssence™.

l Enter the concentrations of the standards.

5 Set up the other experimental parameters. a Click the Monos icon .

The parameters for the monochromators appear.

b Set the parameters.


3-21

6 Click the Run button. The experiment starts.


3-22

Changing the type of microwell plate The MicroMax 384 accepts common microwell plates with 384 microwells and 96 microwells, as well as custom arrays of microwells. To change from one type of plate to another, create a new instrument configuration for each extra type of plate as described in step 5 in the “Requirements & Installation” chapter, pp. 2-6 through 2-10.


3-23

Batch jobs The MicroMax 384 can perform batch jobs automatically using the FluorEs-sence™ software.

1 Set up the MicroMax 384 experiment to be re-peated automatically.

2 In the Experiment area, give it a name in the File field.

3 Click the Save button.

4 In the FluorEssence toolbar, click the Run JY Batch Experiments button . The Setup batch experiments window appears.


3-24

5 Set up the file management.

a Load a previously created batch job with the Load button, or browse for .xml (experiment) files with the Browse for experiment files to >> Add button.

b Add each desired experiment file to the Execution List.

c Reorder or remove the files as necessary using the Delete button, the Up button, and the Down button.

d Add comments about the batch file in the Comments: field.

e Save the new batch job in the correct path, i.e., in the File Name: field, and click the Save button.

6 Set up the various individual experiments.


3-25

a Select the desired experiment in the Execution List.

b In the Total Repeats: field, enter the number of times that experiment should be repeated.

c In the Delay before executing: field, enter the number of seconds to wait before executing.

d In the Delay between each repeat list: field, enter the number of seconds to wait before repeating the experiment.

e Repeat steps a–d for the other experiments in the Execution List.

7 Set up the batch job that runs the various experi-ments.

a In the Total Repeats: field, enter the number of times to repeat the batch job.

b In the Delay before first: field, enter the number of seconds to wait before starting the batch job.

c In the Delay between each: field, enter the number of seconds to wait before rerunning the batch job.

8 Click the Run button to start the batch job.


3-26

MicroMax 384 v. 3.0 (9 Mar 2007) Daily Calibration Verification

4-1

4: Daily Calibration Verification Introduction

HORIBA Jobin Yvon recommends that the operator verify the calibration of the Mi-croMax 384 and its associated spectrofluorometer at the start of each day.

Method 1 Calibrate the spectrofluorometer

Perform the normal calibration recommended for the instrument as described in the Operation Manual. For example, check the excitation output with a xenon-lamp scan, and then the emission using a water-Raman signal in a cuvette.

2 Install the F-3000 or F4-3000 adapter. Remove the existing sample holder for cuvettes, and replace it with the F-3000 or F4-3000 adapter for fiber-optic bundles.

3 Calibrate the MicroMax 384. Verify the microwell-plate alignment of the MicroMax 384 as described in the “Calibrate and Align the MicroMax 384” section of this manual.

MicroMax 384 v. 3.0 (9 Mar 2007) Daily Calibration Verification

4-2

MicroMax 384 v. 3.0 (9 Mar 2007) Tutorials

5-1

5: Tutorials Introduction

To acquaint you with operation of the MicroMax 384, two tutorials are provided in this chapter. The first tutorial shows how to scan blanks and unknown samples to obtain a blank-subtracted spectrum. The second tutorial demonstrates the use of Single Point scans to scan a large number of microwells in a microwell plate at a particular wave-length-pair. Both tutorials assume that the spectrofluorometer and MicroMax 384 already have been set up, calibrated, and run properly.


5-2

Warning: Refer to your Material Safety Data Sheet (MSDS) for information on the hazards of fluorescein and sodium hydroxide.

Tutorial 1: full-spectrum analysis The sample used in this tutorial is fluorescein dissolved in 0.1 N NaOH. Concentrations of 1 µM, 2 µM, 5 µM, 8.3 µM, and 10 µM fluorescein were mixed, along with a blank (0.1 N NaOH). A 96-well microwell plate is used. The fluorescein is in microwells F1–F5, while the blank is in microwell B1. The remainder of the microwell plate is empty.

1 Calibrate the instrument and the MicroMax. 2 Insert the microwell plate (with samples) into the

MicroMax, and close the lid. 3 In the main FluorEssence window, click the Ex-

periment Menu button.



5-3


5 Choose Spectra.


6 Choose Emission, and click Next >>. The Experiment Setup window appears.




5-4

7 Enter test in the Data Identifier field, and give a new File name.

8 Add a Comment describing the experiment.

9 Enter the monochromator parameters. a Park the excitation monochromator at 485 nm.

b Set the excitation Slit to 1 nm.

c Start the scan at 490 nm.

d End the scan at 650 nm.

e Increment the scan 1 nm by entering 1 in Inc.

f Set the emission monochromator Slit to 1 nm.

10 Choose the detector parameters.

a Click the Detectors icon .

b Set the Integration time to 0.1 s


5-5

c Enable detector S1.

11 Activate the MicroMax 384. a Click the Accessories icon .


b Choose the MicroMax or XY Stage tab.

Note: The name of the tab may vary, depending on how the instrument configuration is set.



5-6



5-7

d Choose the Unknown radio button.

e Click on microwell F1, and drag the mouse to microwell F5. As microwells are designated, their position and type appear in the table on the right side of the window.

f Choose the Blank radio button.

g Click on microwell B1. The window above shows many microwells designated for scanning.


5-8

12 Click the Save button or Save As ... button to save this experiment. The software asks for a filename.

13 Click the Run button to run the experiment. The Intermediate Display shows the data appearing in real time. Here the Mi-croMax scans the blank first, then the unknowns.


5-9

When the scanning is com-plete, the Project name win-dow appears.

14 Enter a project name, and click the OK button. The final graphs appear in the window as a 3-D plot.

15 Show the graphs as an overlay plot. a Click File, and choose New.

b The New window appears.

c Click Graph, and then the OK button.

A new graph appears.


5-10

d Right-click on the 1 in the upper-left corner. A menu ap-pears.

e Choose Layer Contents.... The Layer 1 window opens.

f In the left column, highlight the datafiles to display, then click the => after each datafile. The desired files appear in the right column.

g Click the OK button. The Layer 1 window disappears.

h Choose the Rescale button to rescale the graph so that all data appear.


5-11

i Click on each dataset as desired, and choose Color to give it a different color.

Note: For more information about plotting in Origin®, see the Origin® on-line help files.


5-12

Warning: Refer to your Material Safety Data Sheet (MSDS) for information on the hazards of fluorescein and sodium hydroxide.

Tutorial 2: constant-wavelength analysis The sample used in this tutorial is fluorescein dissolved in 0.1 N NaOH. Concentrations of 1 µM, 2 µM, 5 µM, 8.3 µM, and 10 µM fluorescein were mixed, along with a blank (0.1 N NaOH). A 96-well microwell plate is used. The fluorescein is in microwells F1–F5, while the blank is in microwell B1. The remainder of the microwell plate is empty.

1 In the main FluorEssence window, click the Ex-periment Menu button. The Select Hardware Configuration win-dow appears.

2 Choose the appropriate instrument configuration with a MicroMax 384 at-tached.


5-13

The FluorEssence Main Ex-periment Menu opens.

3 Choose Single Point.

The Experiment Setup win-dow appears.

4 Enter the wavelengths to monitor: 485 in the Exci-tation 1 column, then 518 in the Emission 1 col-umn.


Note: If there is only one instrument configuration, this window does not appear.


5-14

5 Set the detector parameters.

a Choose the Detectors icon. The detector parameters appear.

b Enable S1.

c Enter an integration time of 0.1 s.





5-15

b Choose the MicroMax or XY Stage tab. The grayed-out parameters of the MicroMax tab appear:

Note: The name of the tab may vary, depending on how the instrument configuration is set.


5-16


d Choose the Blank radio button.

e Click on microwell B1 in the chart. Microwell B1 turns blue, and it appears in the table on the right.

f Choose the Unknown radio button.

g Click on microwell F1, hold the mouse button down, and drag to microwell F5. Microwells F1 to F5 turn red, and they appear in the table on the right.

h Enter the concentrations of the standards. In the table, click in the desired row, and enter the concentration in the Concentration column. Below is a sample window with many microwells designated for scan-ning:


5-17

7 Click the Run button. The experiment starts, and the Intermediate Display appears:

When the scanning is complete, the Project name window appears.


5-18

8 Enter a project name, and click the OK button. The final spreadsheet ap-pears:.

9 Create a calibration curve. a In the FluorEssenceTM toolbar, click the Create/Use Calibration

Curve from CWA Data button .

The Calibration Curve Pa-rameters window appears.

b Choose the Linear radio button for these samples, then click the OK button. The Save Curve window appears.

c If you wish to save the calibration curve, click the Yes button. The Enter a FileName win-dow appears.


5-19

d Enter the desired filename for the calibration curve, and click the Save button.

e The calibration curve appears.

The correlation coefficent R2 appears in this box.

The standards appear as stars.

The equation for the best fit ap-pears here.

Note: The unknowns do NOT appear on the graph, but are in the spread-sheet.

Note: If you repeat the same experiment, the software will overwrite this graph. Save or rename the graph to keep it.


5-20

MicroMax 384 v. 3.0 (9 Mar 2007) Maintenance

6-1

Warning: Refer to your Material Safety Data Sheet (MSDS) for information on the hazards of acetone or other organic solvents.

Caution: Do not expose the ends of the fiber-optic cables or the ferrules to aqueous solutions, especially acids or bases.

Caution: Never polish the exposed ends of the fibers.

6: Maintenance Care of the fiber-optic cable

To clean the ends of the bifurcated cable,

1 Wet a cotton swab with organic solvent (e.g., ace-tone).

2 Gently wipe the exposed face of the fibers with the solvent-saturated swab.

MicroMax 384 v. 3.0 (9 Mar 2007) Maintenance

6-2

Storage Fiber-optic cable and F-3000 or F4-3000 adapter

1 Cover the open ferrules on the ends of the fiber-optic cables with the plastic shipping caps.

2 Store fiber-optic cable and adapter in a drawer or cabinet.

MicroMax 384

1 Disconnect the AC (mains) power cord. 2 Disconnect the RS-232 communication cable. 3 Remove the microwell plate. 4 Remove the fiber-optic assembly. 5 Close the lid.

Caution: Avoid twisting the fiber-optic bundles too much when stored. Breakage of the glass fibers may occur with excessive strain.

MicroMax 384 v. 3.0 (9 Mar 2007) Troubleshooting

7-1

7: Troubleshooting If you suspect that fibers in the fiber-optic cable are broken,

1 Hold the single end of the bifurcated cable up to a bright light.

2 View each end of the bifurcated side carefully to see that the light is transmitted through the cable. Verify that nearly all of the fibers are lit up, and that there are few—if any—broken fibers.

Caution: Never pull the cable itself—the ferrule may loosen from the cable. Hold the cable by the metal ferrules only.

MicroMax 384 v. 3.0 (9 Mar 2007) Troubleshooting

7-2

MicroMax 384 v. 3.0 (9 Mar 2007) Technical Specifications

8-1

Note: For 24-microwell (6 × 4) and 6-microwell (3 × 2) plates, con-sidered non-standard sizes, contact Spex® Fluorescence Service.

8: Technical Specifications Computer requirements

973005 or equivalent PC with free RS-232 port

Software requirements • Windows® 2000 or XP Pro • FluorEssence™ version 2.1

Physical specifications • 9″ × 12″ × 7″ (deep × wide × tall) • 20.3 cm × 30.5 cm × 17.8 cm (deep × wide × tall) • Allow an extra 3″ (7.6 cm) of height for fiber-optic bundle. • Compatible with Fluorolog®-3 and FluoroMax®-4 • Accepts 384-microwell (24 × 16) and 96-microwell (12 × 8) plates.

• Scans 96 microwells in < 1 min • Typical sensitivity = 25 pM fluorescein with the Fluorolog®-3-22; 10 nM fluo-

rescein with the FluoroMax®-4.

Electrical specifications 110 VAC at 0.5 A and 60 Hz, single-phase 220 VAC at 0.25 A and 50 Hz, single phase

Fiber-optics’ specifications • Bifurcated bundle with slit-ends • Randomized fiber distribution in bundle • Length = 1 m • Numerical aperture = 0.12 NA • Wavelength range covers full range from 250–850 nm. Other wavelength ranges are

available.

MicroMax 384 v. 3.0 (9 Mar 2007) Technical Specifications

8-2

MicroMax 384 rev. E (28 Nov. 2011) Compliance Information

9-1

9: Compliance Information Declaration of Conformity

Manufacturer: HORIBA Jobin Yvon, Inc.

Address: 3880 Park Avenue Edison, NJ 08820 USA

Product Name: Model Names:

MicroMax MicroMax-384

Conforms to the following Standards: Safety: EN 61010-1: 2001

EN 61010-1: 2001/AC: 2002 EMC: EN 61326-1: 2006 (Emissions & Immunity)

Supplementary Information The product herewith complies with the requirements of the Low Voltage Directive 2006/95/EC and the EMC Directive 2004/108/EC. The CE marking has been affixed on the device according to Article 8 of the EMC Directive 2004/108/EC. The technical file and documentation are on file with HORIBA Jobin Yvon, Inc.

______________________________ Salvatore Atzeni Vice-President, Retail Engineering HORIBA Jobin Yvon, Inc. Edison, NJ 08820 USA Nov. 14, 2011

MicroMax 384 rev. E (28 Nov. 2011) Compliance Information

9-2

Applicable CE Compliance Tests and Standards Test Standards

Emissions, Radiated/Conducted EN 55011: 2006 Radiated Immunity IEC 61000-4-3: 2006 Conducted Immunity IEC 61000-4-6: 2008 Electrical Fast Transients IEC 61000-4-4: 2004 Electrostatic Discharge IEC 61000-4-2: 2008 Voltage Interruptions IEC 61000-4-11: 2004 Surge Immunity IEC 61000-4-5: 2005 Magnetic Field Immunity IEC 61000-4-8: 2009 Harmonics IEC 61000-3-2: 2006 Flicker IEC 61000-3-3: 2008 Safety EN 61010-1: 2001

EN 61010-1: 2001/AC: 2002

MicroMax 384 v. 3.0 (9 Mar 2007) Index

10-1

10: Index Key to the entries: Times New Roman font.........subject or key-

word Arial font ................................command,

menu choice, or data-entry field

Arial Condensed Bold font.....dialog box Courier New font..............file name or

extension

=

=> button .................................................5-10

3

3-D plot ......................................................5-9 3D scan-type ..............................................3-7

6

650025 .......................................................2-2 650026 .......................................................2-2

8

81051 .................................................... ii, 2-2

9

97133 .........................................................2-2 97134 .........................................................2-2 973005 ............................................... 2-1, 8-1 98015 .........................................................2-2

A

absorbance ..................................... 2-22, 2-30 Accessories icon.. 2-15, 2-19, 2-23, 2-26, 2-

31, 2-34, 3-2, 3-5, 3-9, 3-16, 5-5, 5-14 acetone .......................................................6-1

Activate checkbox ...2-17, 2-24, 2-32, 3-3, 3-11, 3-18, 5-6, 5-16

Add >> button......................................... 2-11 Allen key ......................................... 2-5, 2-20

B

baffle.......................................................... 2-3 Band Pass ................................................ 2-19 batch file .................................................. 3-24 batch job ...................................3-23–24, 3-25 Blank radio button......... 3-12, 3-19, 5-7, 5-16 blank subtraction ..................................... 3-14 Browse for experiment files to >> Add

button................................................... 3-24

C

cable............................. 2-2–3, 2-6–7, 6-2, 7-1 calibration.........................................4-1, 5-19 calibration curve ................................ 5-18–19 Calibration Curve Parameters window ............. 5-18 caution notice ............................................ 1-4 Clear All button..............................3-12, 3-19 Collect menu............................................. 2-8 Color ....................................................... 5-11 COM port ................................. 2-1, 2-6, 2-10 Comment ................................................... 5-4 Comments field ..................................... 3-24 computer requirements .......................2-1, 8-1 Concentration column ............................. 5-16 concentration curves................................ 3-14 Concentration field .................................. 3-13 Configuration Name window......................... 2-10 constant-wavelength analysis .........3-14, 5-12 correction files......................................... 3-14 cotton swab................................................ 6-1 Create/Use Calibration Curve from CWA

Data button .......................................... 5-18 Current Cell field .................. 2-27, 2-35, 3-6 Curve Fitting area..........................3-13, 3-20


10-2

D

damage ................................1-3–5, 1-7–8, 2-2 danger to fingers notice............................. 1-4 Data Identifier field................................... 5-4 Declaration of Conformity........................ 9-1 Delay before executing field................... 3-25 Delay before first field ............................ 3-25 Delay between each field ........................ 3-25 Delay between each repeat list field ....... 3-25 Delete button.......................................... 3-24 Delete Row(s) button................... 3-13, 3-20 dental mirror...................................... 2-20–21 Detectors icon................................. 5-4, 5-14 Dialog window......................................... 2-10 disclaimer.................................................. 1-2 Down button ........................................... 3-24

E

Emission 1 column................................ 5-13 Empty radio button........................ 3-12, 3-19 End ........................................................... 5-4 Enter a FileName window ............................ 5-18 excessive humidity notice ......................... 1-5 Excitation 1 area ........................... 2-28, 2-36 Excitation 1 column............................... 5-13 excitation port ....................................... 2-4–5 Execution List ................................... 3-24–25 Experiment area ...................................... 3-23 Experiment Menu button . 3-7, 3-14, 5-2, 5-

12 Experiment Menu button ........................ 2-13 Experiment Setup...................................... 2-8 Experiment Setup window 2-14, 2-22, 2-31, 3-

1, 3-7–8, 3-15, 5-3, 5-13 Experiment Type window. 2-14, 2-22, 2-31, 3-

1, 3-8, 5-3

F

F-3000.2-1–4, 2-6, 2-11–13, 2-20–21, 4-1, 6-2

F4-3000... 2-1–4, 2-6, 2-11–12, 2-20–21, 4-1, 6-2

face-shield notice ...................................... 1-5 ferrule........................................2-3, 2-21, 6-2

fiber-optic adapter .................... 2-1, 2-3, 2-37 fiber-optic bundle . 1-1, 2-1, 2-4, 2-6, 4-1, 8-1 fiber-optic cable.............2-2, 2-20, 6-1–2, 7-1 fiber-optic interface ...................................2-2 File.............................................................5-9 File field ..................................................3-23 File Name field.......................................3-24 Finish button ...........................................2-11 fluorescein ..............2-22, 2-30, 5-2, 5-12, 8-1 FluorEssence icon.............................2-8, 2-13 FluorEssence Main Experiment Menu ...2-14, 2-

22, 2-30, 3-1, 3-8, 3-15, 5-3, 5-13 FluorEssence window .2-8, 2-13, 3-7, 3-14, 5-

2, 5-12 FluorEssenceTM toolbar ..................3-23, 5-18 FluorEssence™ i, 1-1, 2-1–2, 2-8, 2-13, 2-15,

2-19, 2-22–23, 2-26, 2-30–31, 2-34, 3-1–2, 3-5, 3-9, 3-13, 3-16, 3-20, 3-23, 8-1

Fluorolog® .............................. 1-1, 2-1–3, 8-1 Fluoromax Configuration window ..................2-10 FluoroMax® .....................1-1, 2-1–2, 2-5, 8-1

G

gasket.....................................................2-4–5 General icon ..................................2-27, 2-35 Graph .........................................................5-9

H

Home button .............................................3-6 Home position...........2-21–22, 2-30, 3-1, 3-6 host computer . 2-1, 2-3, 2-6, 2-13, 2-21, 2-30

I

I/O connector .............................................2-6 infrared radiation ...................................1-8–9 instrument configuration . 2-8, 2-10–11, 2-14,

2-22, 2-30, 3-1, 3-8, 3-15, 3-22, 5-3, 5-12 integration time...............................3-14, 5-14 Integration time .........................................5-4 intense light notice.....................................1-4 Intermediate Display ...............................5-8, 5-17


10-3

K

kinetics .....................................................3-14

L

Layer 1 window ........................................5-10 Layer Contents... ......................................5-10 lens .......................................1-6, 1-9, 2-3, 2-7 Lens assembly............................................2-2 light-sensitive samples.............................3-14 line voltage..............................2-2, 2-6–7, 6-2 Linear radio button..................................5-18 Load button .............................................3-24

M

mains.......................................2-2, 2-6–7, 6-2 Material Safety Data Sheets.......................1-2 MicroMax checkbox................................2-10 MicroMax Communication Settings window .....2-10 MicroMax tab .. 2-16–17, 2-24, 2-26, 2-32, 2-

34, 3-3, 3-5, 3-10–11, 3-17–18, 5-5–6, 5-15–16

microwell 2-11, 2-17, 2-21–22, 2-24, 2-27, 2-30, 2-33, 2-35, 2-37, 3-1, 3-3, 3-6, 3-12, 3-19, 3-22, 4-1, 5-1–2, 5-7, 5-12, 5-16

microwell plate 1-1, 2-2, 2-11, 2-21–22, 2-27, 2-30, 2-35, 2-37, 3-1, 3-6, 3-22, 4-1, 5-1–2, 5-12, 6-2, 8-1

monochromator.1-1, 2-3, 2-19, 2-28, 2-36, 5-4

monochromators ......................................3-20 Monos icon .............................................3-20 MSDS ........................................................1-2

N

NaOH.............................2-22, 2-30, 5-2, 5-12 New ...........................................................5-9 New button ................................................2-9 New window...............................................5-9 Next > button . 2-9–10, 2-14, 2-22, 2-31, 3-1 Next >> button 2-9, 2-14, 2-22, 2-31, 3-1, 3-

8, 5-3 numerical aperture .................................... 8-1

O

OK button................... 2-10–11, 5-9–10, 5-18 Open ..............................................2-28, 2-36 overlay plot................................................ 5-9

P

Park........................................................... 5-4 photobleach mode.................................... 3-14 physical requirements................................ 2-1 plastic plug .........................................2-3, 2-5 Position..........................................2-28, 2-36 power switch............................................ 2-13 project name .....................................5-9, 5-18 Project name window........................5-9, 5-17 protective gloves notice............................. 1-5

R

read this manual notice.............................. 1-5 Real Time Control.. 2-17–18, 2-24–25, 2-33, 3-

1, 3-3–4 rectangular plate ........................................ 2-3 Rescale button ....................................... 5-10 RS-232................................. 2-2, 2-6, 6-2, 8-1 RTC button.................... 2-18, 2-25, 2-33, 3-4 Run button . 2-28, 3-13, 3-21, 3-25, 5-8, 5-17 Run JY Batch Experiments button .......... 3-23

S

S1 .....................................................5-5, 5-14 safety ......................................................... 1-4 safety goggles notice ................................. 1-5 safety precautions ...................................... 1-4 sample compartment...............2-3–6, 2-19–21 sample-compartment cover ..................... 2-29 Save As ... button....................................... 5-8 Save button....................... 3-23–24, 5-8, 5-19 Save Curve window.................................. 5-18 Select All button ............................3-12, 3-19 Select Hardware Configuration window .2-8, 2-

13, 3-8, 3-15, 5-2, 5-12 sensitivity................................................... 8-1 Setup batch experiments window.................... 3-23 shutter .......................... 2-19, 2-28, 2-36, 3-14


10-4

Shutter Mode switch .................... 2-28, 2-36 Signal Intensity tab ........................ 2-28, 2-36 Single Point.................................... 3-15, 5-13 Single Point scans .......................... 3-14, 5-1 Slit ............................................................. 5-4 Slits ................................................ 2-28, 2-36 software requirements............................... 8-1 Spectra....................................................... 5-3 Standard .................................................. 3-13 Standard Instrument Configuration radio

button .................................................... 2-9 Standard radio button................... 3-12, 3-19 Start ........................................................... 5-4 Summary window .................................... 2-11 symbols ..................................................... 1-4 System Configuration Wizard window .............. 2-9 System Selection Page window........................ 2-9

T

thumbscrew............. 2-3–4, 2-6–7, 2-21, 2-37 Total Repeats field .................................. 3-25 Total Repeats ield ................................... 3-25

U

ultraviolet exposure................................... 1-6 ultraviolet light notice ............................... 1-4 Unknown radio button . 3-12, 3-19, 5-7, 5-16 Up button ................................................ 3-24

UV training................................................1-7

V

visible radiation .........................................1-8

W

warning notice ...........................................1-4 water-Raman..............................................4-1 wavelength-pair ........................ 1-1, 3-14, 5-1 Windows®...................................... ii, 2-1, 8-1

X

X Origin...................................................2-11 xenon lamp .........................................1-8, 1-9 xenon lamp risks........................................1-8 xenon-lamp scan........................................4-1 .xml .......................................................3-24 XY Stage tab ...................................5-5, 5-15

Y

Y Origin...................................................2-11 Yes button ...............................................5-18

[Design Concept]

The HORIBA Group application images are collaged in the overall design. Beginning from a nano size element, the scale of the story develops all the way to the Earth with a gentle flow of the water.

HORIBA JOBIN YVON INC. 3880 Park Ave., Edison, New Jersey 08820-3012, U.S.A. http://www.jobinyvon.com

Documents

MicroMax 384 v. 3.0 (9 Mar 2007) - Horiba · The MicroMax 384 is a microwell-plate reader able to accept plates with up to 384 wells. It connects to the FluoroMax® and Fluorolog®