Online bacteria monitoring is possible……..

now…,

The Role of Sensors in Disinfection and

Microbiological Monitoring

Joep Appels / microLAN BV

microLAN “CONTROL”-Line Monitoring Systems, for Early Warning and Trend Monitoring of

Algae, Bacteria & Chemicals

BE IN CONTROL OF YOUR WATER QUALITY!

What customers want

microLAN B.V.

microLAN: micro Luminescence Analysis Netherlands:

microLAN BV: Waalwijk, the Netherlands

microLAN China Ltd Hong Kong

Market leader in non-chemical monitoring

1st on-line Toxicity monitor using luminescent bacteria (2004)

- 200+ installations world-wide

- Key references with US EPA, Veolia, Suez, SAB Miller etc.

Unique systems for monitoring of toxicity of chemical contaminations:

- water intake protection -> Drinking water / food

- river water monitoring -> Rhine, Danube, Yellow River

- wastewater monitoring (influent / effluent).

Extension of products:

2013 ALGcontrol: Fluorescence monitoring of algae classes and toxic algae

2015 BACTcontrol: Enzymatic monitoring of bacteria growth

CONTROL Monitoring product Line

iTOX control

ALG control

BACT control

• CHEMICALS

• On-line toxicity monitoring for chemical screening

• ALGAE

• Fluorescence monitoring of algae classes and toxic algae

• BACTERIA

• Enzymatic monitoring of bacteria growth

Data handling/ transfer

Water inflow

Chemicals Monitoring

iTOXcontrol

Algae Monitoring

ALGcontrol

Bacteria Monitoring

BACTcontrol

High Frequency data with Low quality:

trend monitoring / early warning

High Frequency / Low Quality data

Control

Online microbiological monitoring

Patented trend monitor

and Early Warning System

for microbiological

(Faecal) contamination

Enzymatic reaction with

fluorescence detection

Two ranges:

– Low level clean water application: 2-3 h detection, bigger

sample volume trough concentration (up to 3-5L) better

statistics compared to lab methods

– High level surface / waste water (no concentration

needed): < 1h detection

Specifications

Based on Fluorescent measurement of

specific enzymatic activity:

– ß-Glucuronidase -> E.coli

– ß-Galactosidase -> Coliforms

– Alkaline Phosphatase -> Total Activity

– Enterokokken (2015 with KWR)

Sample volume: 100 – 5.000 ml

Detection: 1-5 cfu per 100ml

(depending on sample volume)

Short measuring intervals (2-3 hours)

up to 6 per day.

Filter changing: 75 to 450 measuring

cycles (depending on the turbidity).

Without the filter >5 - 10.000 cfu

in < than 1 hour

Measurement sequence

Init. Filtration Measurement Cleaning Data

<60

min

~60

min

0 min ~5 min 1 - 300

min

2 – 6 h measuring cycle (express 1h)

Heating of reactor to

36/44°C

Buffer dosing

Reagent dosing

Stabilization

Stepwise measurement

of fluorescent light System Filling (Bypass)

Rinsing of cleaning liquid

Initial dosing

Rinsing of Initial dosing

All pumping operations

through the filter in reverse

direction.

Duration depends

upon volume und

condition of filter.

0.2 – 1 ml/s

Calculation

Heating of reactor to

65°C (no heating in

express mode)

Disinfection of system

with chlorine solution

BACTcontrol measurement

Enzymatic bacteria monitoring (BACTcontrol) compared with Flow Cytometry.

Groundwater monitoring

Drinking water monitoring

A Danish drinking water facility determining a contamination using the

BACTcontrol

The graph shows an increase in enzyme activity date 6/5-2014 and suddenly

a high peak in coliform activity. The water work reacted on the first rise in

activity, and water from the water work was not used as drinking water, when

the high contamination occurred.

Waste water monitoring

In Spain, the BACTcontrol is used to study the reliability, the functionality and the

possible correlation between the online results with laboratory results, on a waste

water treatment plant.

Some conclusions are that there is a good correlation between online - and

laboratory results(as shown in the graph), and with the use of the online BACTcontrol

any method of disinfection can be automated.

Blue line is BACTcontrol, red line are lab results, green line is flow.

Food & Beverage applications

Test and

R&D location

for food

applications

De

Waterfabriek

Netherlands

Ongoing R&D projects with renowned institutes

Aquavalens

– EU project with validation by TZW (D) and Agbar (Sp)

KWR

– Development of enterokokken application

Brabant Water

– Validation food application

Waterboard NL

– Waste Water testing and Watereuse

Vitens

– Different applications and data integration

Conclusions

Online bacteria (growth) monitoring is possible

now (probably even with some of our

competitors…)

Can they replace traditional (lab) methods ?

– No but enhance (less testing needed)

Possibilities are now to:

– Dose less chlorine (like none in NL)

– Use less energy (UV, Ozone etc.)

– Decrease water use

MICROLAN “CONTROL”-LINE MONITORING SYSTEMS To be “IN CONTROL” of your water quality / security !

Thank you, for questions pls mail to info@microlan.nl

Or look at our website www.microlan.nl