Labeled Iba1 AntibodyIba1 is a protein highly expressed in microglia and macrophage with a molecular weight of about 16.7kDa The protein is a commonly known microglial marker in the nervous system. These items are Anti Iba1, Rabbit (for Immunocytochemistry): Code No. 019-19741, labeled with Biotin or Red Fluorochrome like Cy5TM

Assay ConditionSample

Cerebral cortex of 7-week Wistar rat or 7-week ICR mouseSection

50μm- frozen section Antibody concentration 1/200

Anti Iba1, Rabbit Code No.

019-19741

Secondary antibody

biotin-conjugated

Anti Iba1, Rabbit, Biotin-conjugated

Rat Mouse

Anti Iba1, Rabbit, Red

Fluorochrome(635)-conjugate

Assay ConditionSample

Cerebral cortex of 7-week Wistar rat or 7-week ICR mouseSection

50μm- frozen section Antibody concentration 1/200

The secondary antibody process could be eliminated

Evaluate by immunohistochemistry test

Reduced background

Applications –Immunohistostaining-

Rat Mouse

Anti Iba1, Rabbit, Biotin-conjugated Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated

Features

The item shows clearer localization signals of microglia than using secondary antibody.

All immunohistochemistry data was provided by Sanagi ,T. , Ichinohe ,N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

Anti Iba1, Rabbit Code No.

019-19741

Secondary antibody Alexa Fluor® 647

-conjugated

The item shows the localization signals of microglia as well as using secondary antibody.

Microglial marker

Anti Iba1, Rabbit, Biotin-conjugated

Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated

Conjugate Biotin Red Fluorochrome (Excitation=634nm, Emission=654nm)

Antigen Synthetic peptide corresponding to C-terminus of Iba1

Buffer PBS with 0.05% Sodium Azide

Species cross reactivity Mouse, Rat, and Marmoset Mouse and Rat

Application Immunohistochemistry 1:200-2,000

Antibody information

Product list

Product Name Wako Cat. No Pkg. Size Grade Storage Condition

Anti Iba1, Rabbit (for Immunocytochemistry) 019-19741 50 g For Immunochemistry Keep at -20 .

Anti Iba1, Rabbit (for Western Blotting) 016-20001 50 g For Immunochemistry Keep at -20

Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) 015-25191 50μL For

Immunochemistry Keep at -20

Anti Phosphorylated α-Synuclein, Monoclonal Antibody(pSyn#64), Biotin-conjugated 010-26481 100μL For

Immunochemistry Keep at 2-10 .

Anti Phosphorylated α-Synuclein, Monoclonal Antibody(pSyn#64), FITC-conjugated 017-26491 100μL For

Immunochemistry Keep at 2-10 .

Related Products

Product Name Wako Cat. No Pkg. Size Grade Storage Condition

Anti Iba1, Rabbit, Biotin-conjugated 016-26461 100 For Immunochemistry Keep at 2-10 .

Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated 013-26471 100 For Immunochemistry Keep at 2-10 .