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UDEE 2114 MICROBIOLOGY
YEAR 2 SEMESTER 3
Name : Hemalatha Kannan
Student ID : 09ADB07509
Partners name: Ngoi Jiang Chin
Hing Yi Shien
Yap Ye Vone
Chew Hui Sin
Sanmugapriya Elamparuthi
Faculty : Science
Course : Biochemistry
Session : 2009/2010
Lecturer : Miss Teo Kah Cheng
Date : 1st March 2011
Title: The determination of microbial numbers
Objective(s) :1) To determine the microbial number using spread plate method.
2) To determine the number of bacteria on the plate using the dilution dilution
factor.
Results
Spread Plate
Part A : Removal of 0.1ml from the dilution tube
Dilution Results Observation10-1
Confluent growth of bacteria.4 single colonies is observed.
10-2
Confluent growth of bacteria. Many small single colonies present.
10-3
Many single colonies are present. The growth is still confluent.
10-4
The number of single colonies of bacteria has reduced but the colonies are still more than 200 colonies.
10-5
The number of single colonies is few but still it is more than 200 colonies.
Part B : Removal of 1.0ml from the dilution tube
Dilution Results Observation 10-1
The growth of bacteria is very confluent. No single colonies present.
10-2
The growth of bacteria is very confluent. No single colonies present.
10-3
The growth of bacteria is very confluent. No single colonies present.
10-4
Single colonies are present at the edge of the agar plate. The growth of bacteria is still confluent.
10-5
Many single colonies of bacteria is present. The number of colonies present is more than 200.
Discussion
The main purpose of streak plate method is to form well-isolated colonies so that pure culture can be obtained. As a results, isolated colonies were obtained at the end of streak in each dilution. For the spread plate with 0.1ml
References
1. UDEE 2114 Microbiology Lab Manual, Experiment 1 and 3.
2. http://inst.bact.wisc.edu/inst/index.php. Microbiology. Retrieved on 10th March 2011.
3. Karren E. Messley and Stephen A.Norrell.(2003). Microbiology Laboratory Manual.
Prentice Hall.
