Microbiology Module 7 - Bacilli

7/30/2019 Microbiology Module 7 - Bacilli

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Microbiology HTL

Bacilli

Gram (+) non-spore forming

Gram (+) spore formingGram (-) bacilli


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Microbiology HTL

Taxonomical Classification:

Gram (+) non-sporeformers

Mycobacterium

Corynebacterium

gravis

mitis

Intermedius

Lactobacillus

Erysipelothrix

Listeria

Gram (+) spore formers:Aerobic - Bacillus

B. anthracis

B. subtilisAnaerobic-Clostridium

C. tetani

C. botulinum

C. perfringens


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Microbiology HTL

Taxonomical Classification: (2)Gram (-) bacilli -

Enterics

Escherichia

Salmonella

Shigella

Klebsiella

Arizona

Providencia

Vibrio

Citrobacter

Edwardsiella

Serratia

Proteus

Morganella


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Microbiology HTL

Small gram (-) bacilli

Bordatella

Hemophilus

Brucella

Pasteurella


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Microbiology HTL

Mycobacterium: (1)M. tuberculosis

(Kochs bacilli)

long, slender,

granular curved orrounded end

AFS (+), Gm (+)

*glycerineLowenstein,

Petroffs, Dorsets ,

Corpers

Types:

Human -man

Bovine - hogs &

cattle

Avian - birds

Pathogenecity: no

toxins produced

Productive Lesions

Exudative Lesion


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Microbiology HTL

AFS property of Mycobacterium:

Due to the presence of mycolic acid.

Mycolic acid gives M. tuberculosis many

characteristics that defy medical treatment.They lend the organisms increased resistance

to chemical damage & dehydration.

Prevent the effective activity of hydrophobicantibiotics

Allows it to grow within macrophages which

makes it resistant to the immune system action


7/87Microbiology HTL
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Mycobacterium (2)Types of tuberclosis

Primary infection

Reactivation TB

Millary TB

Diagnosis:

microscopic exam

chest x-ray

tuberculin test

culture of sputum

M. leprae ( Lepraebacilli, Hansens bacilli)

long, slender, usually

straight, non-motile,grouped like cigarette

packets

Pathognesis: Leprosy

Acute nodular

Anesthetic or nerve


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Mycobacterium (3) transmission:

prolonged contact

organisms found in

mucous membrane

diagnosis: Mitsuda,

Lepromin test

Stains:Ziehl Neelsen

Baumgarten

Papenheims

M. leprae ( Leprae

bacilli, Hansens

bacilli)

grouped like packetof cigarettes

leprosy:

acute nodular -leproma

anesthetic - nerve

leprosy


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Corynebacterium: (1)

C.diptheriae: KlebLoeflers bacilli

pleomorphic, globular,

club shaped, bard-shaped,irregular swelling &

staining ---Babe Ernst

bodies

Gram (+), AFS (-)

Culture: BAP, Pais

Tellurite

more in children

Pathogenesis: toxin

is absorbed --

destruction of cells --inflammatory

response -- formation

of pseudomembrane

-- bleeding -- edema

Specimen : nose &

throat swabs


http://rds.yahoo.com/S=96062857/K=corynebacterium+diphtheria/v=2/SID=w/l=II/R=15/SS=p/OID=3d842a5120b96392/SIG=1iugvco8v/EXP=1122533906/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dcorynebacterium%2Bdiphtheria%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-1520085065_q-ZxC94cgHw7ncBI%2Fq9ZIpxAABAA%40%40&h=264&w=410&imgcurl=tooldoc.wncc.edu%2FInfections%2Fanalwart.JPG&imgurl=tooldoc.wncc.edu%2FInfections%2Fanalwart.JPG&size=11.4kB&name=analwart.JPG&rcurl=http%3A%2F%2Ftooldoc.wncc.nevada.edu%2Fmanifest.htm&rurl=http%3A%2F%2Ftooldoc.wncc.nevada.edu%2Fmanifest.htm&p=corynebacterium+diphtheria&type=jpeg&no=15&tt=70&ei=UTF-8http://rds.yahoo.com/S=96062857/K=corynebacterium+diphtheria/v=2/SID=w/l=II/R=3/SS=p/OID=82c56fe438996a0e/SIG=1obmcbll4/EXP=1122533906/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dcorynebacterium%2Bdiphtheria%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-1520085065_q-ZxC94cgHw7ncBI%2Fq9ZIpxAABAA%40%40&h=254&w=271&imgcurl=www.kcom.edu%2Ffaculty%2Fchamberlain%2FWebsite%2Flectures%2Flecture%2Fimage%2Fdiphth2.gif&imgurl=www.kcom.edu%2Ffaculty%2Fchamberlain%2FWebsite%2Flectures%2Flecture%2Fimage%2Fdiphth2.gif&size=76.3kB&name=diphth2.gif&rcurl=http%3A%2F%2Fwww.kcom.edu%2Ffaculty%2Fchamberlain%2FWebsite%2Flectures%2Flecture%2Furiphyn.htm&rurl=http%3A%2F%2Fwww.kcom.edu%2Ffaculty%2Fchamberlain%2FWebsite%2Flectures%2Flecture%2Furiphyn.htm&p=corynebacterium+diphtheria&type=gif&no=3&tt=70&ei=UTF-8http://rds.yahoo.com/S=96062857/K=corynebacterium+diphtheria/v=2/SID=w/l=II/R=1/SS=p/OID=6e2f6213af2c4edc/SIG=1m2dbpsab/EXP=1122533906/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dcorynebacterium%2Bdiphtheria%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-1520085065_q-ZxC94cgHw7ncBI%2Fq9ZIpxAABAA%40%40&h=113&w=149&imgcurl=nengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Fcorynebacterium-diphtheria.jpg&imgurl=nengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Fcorynebacterium-diphtheria.jpg&size=5.0kB&name=corynebacterium-diphtheria.jpg&rcurl=http%3A%2F%2Fnengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Ffm04-5.htm&rurl=http%3A%2F%2Fnengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Ffm04-5.htm&p=corynebacterium+diphtheria&type=jpeg&no=1&tt=70&ei=UTF-8http://rds.yahoo.com/S=96062857/K=corynebacterium+diptheria/v=2/SID=w/l=II/R=3/SS=p/OID=ce61edfdb1de672a/SIG=1j06tgo07/EXP=1122533859/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dcorynebacterium%2Bdiptheria%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=320&w=400&imgcurl=health.yahoo.com%2Fimages%2Fhealth%2Fadam%2Ffullsize%2F9277.jpg&imgurl=health.yahoo.com%2Fimages%2Fhealth%2Fadam%2Ffullsize%2F9277.jpg&size=15.8kB&name=9277.jpg&rcurl=http%3A%2F%2Fhealth.yahoo.com%2Fhealth%2Fency%2Fadam%2F002031%2Fi9277&rurl=http%3A%2F%2Fhealth.yahoo.com%2Fhealth%2Fency%2Fadam%2F002031%2Fi9277&p=corynebacterium+diptheria&type=jpeg&no=3&tt=4&ei=UTF-8http://rds.yahoo.com/S=96062857/K=corynebacterium+diptheria/v=2/SID=w/l=II/R=2/SS=p/OID=43a63d792be0bfb8/SIG=1jj0b6g5q/EXP=1122533859/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dcorynebacterium%2Bdiptheria%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=112&w=90&imgcurl=www.arches.uga.edu%2F%7Ebais%2Fdiptheria%2520disease%2520%282%29.jpg&imgurl=www.arches.uga.edu%2F%7Ebais%2Fdiptheria%2520disease%2520%282%29.jpg&size=3.8kB&name=diptheria%20disease%20(2).jpg&rcurl=http%3A%2F%2Fwww.arches.uga.edu%2F%7Ebais%2Fcorynebacterium.html&rurl=http%3A%2F%2Fwww.arches.uga.edu%2F%7Ebais%2Fcorynebacterium.html&p=corynebacterium+diptheria&type=jpeg&no=2&tt=4&ei=UTF-8http://rds.yahoo.com/S=96062857/K=corynebacterium+diptheria/v=2/SID=w/l=II/R=1/SS=p/OID=b09311c7a85506e6/SIG=1i12muake/EXP=1122533859/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dcorynebacterium%2Bdiptheria%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=130&w=87&imgcurl=www.arches.uga.edu%2F%7Ebais%2Fdiptheria%2520disease.jpg&imgurl=www.arches.uga.edu%2F%7Ebais%2Fdiptheria%2520disease.jpg&size=3.0kB&name=diptheria%20disease.jpg&rcurl=http%3A%2F%2Fwww.arches.uga.edu%2F%7Ebais%2Fcory2.html&rurl=http%3A%2F%2Fwww.arches.uga.edu%2F%7Ebais%2Fcory2.html&p=corynebacterium+diptheria&type=jpeg&no=1&tt=4&ei=UTF-8


14/87

Microbiology HTL


Diagnosis:direct

microscopic exam

culturevirulence test -in

vivo & in vitro

DPT vaccine:Diptheria Pertussis

& Tetanus

combination

Methods ofAcquired immunity

fetal transfer

attack orinfection

Artificial

immunizationfrom infection


15/87

Microbiology HTL


gravis mitis intermedius

short in

tellurite

long , granular

in tellurite

long , barred

in tellurite

(-) hemolysis (+) hemolysis (-) hemolysis

large colonies small colonies small colonies

forms pellicle

in broth

grows

diffusely

granular

sediments


16/87

Microbiology HTL

LACTOBACILLUS

-Most species of this non-spore-formingbacterium ferment glucose into lactose,

hence the nameLactobacillus.

-The most common application ofLactobacillus is industrial, i.e. for dairy

production.

-This genus also contains several bacteriathat make up part of the natural flora of the

human vagina..


17/87

Microbiology HTL

Because of their ability to derive lactic acidfrom glucose, these bacteria create an

acidic environment which inhibits growthof many bacterial species which can lead tourogenital infections.

Lactobacillus is generally harmless tohumans, rarely inciting harmful infectionsor diseases.

Treatment of this vancomycin-resistantmicrobe usually consists of high doses ofpenicillin in combination with gentamicin


18/87

Microbiology HTL
http://rds.yahoo.com/_ylt=A9G_Rq7yD21EuUsAhZGJzbkF;_ylu=X3oDMTBjYzZubXM2BHBvcwM4BHNlYwNzcg--/SIG=1i660f029/EXP=1148084594/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlactobacillus%2526fr%253DFP-tab-img-t%2526toggle%253D1%2526cop%253D%2526ei%253DUTF-8%26w=400%26h=500%26imgurl=skywind.wkhc.ac.kr%252F%2525B9%2525CC%2525BB%2525FD%2525B9%2525B0%252FLactobacillus%252520plantarum.jpg%26rurl=http%253A%252F%252Fskywind.wkhc.ac.kr%252F%2525B9%2525CC%2525BB%2525FD%2525B9%2525B0%252Fmicrow.htm%26size=39.3kB%26name=Lactobacillus%20plantarum.jpg%26p=lactobacillus%26type=jpeg%26no=8%26tt=9,127%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq7yD21EuUsAgJGJzbkF;_ylu=X3oDMTBjcXBoZjEwBHBvcwMzBHNlYwNzcg--/SIG=1eee4aq9t/EXP=1148084594/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlactobacillus%2526fr%253DFP-tab-img-t%2526toggle%253D1%2526cop%253D%2526ei%253DUTF-8%26w=710%26h=710%26imgurl=users.utu.fi%252Fartouwe%252FLGG.JPG%26rurl=http%253A%252F%252Fusers.utu.fi%252Fartouwe%252Ffunctionalfoods.html%26size=44.0kB%26name=LGG.JPG%26p=lactobacillus%26type=jpeg%26no=3%26tt=9,127%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq7yD21EuUsAh5GJzbkF;_ylu=X3oDMTBkYTNuNGk0BHBvcwMxMARzZWMDc3I-/SIG=1ga459v85/EXP=1148084594/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlactobacillus%2526fr%253DFP-tab-img-t%2526toggle%253D1%2526cop%253D%2526ei%253DUTF-8%26w=135%26h=135%26imgurl=www.laktonova.de%252Flactobacillus_1.jpeg%26rurl=http%253A%252F%252Fwww.laktonova.de%252FProbiotische_Kautabletten_Druc%252Fprobiotische_kautabletten_druc.html%26size=6.1kB%26name=lactobacillus_1.jpeg%26p=lactobacillus%26type=jpeg%26no=10%26tt=9,127%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq7yD21EuUsAipGJzbkF;_ylu=X3oDMTBkM2llMThuBHBvcwMxMwRzZWMDc3I-/SIG=1gvn5ot4b/EXP=1148084594/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlactobacillus%2526fr%253DFP-tab-img-t%2526toggle%253D1%2526cop%253D%2526ei%253DUTF-8%26w=198%26h=170%26imgurl=biology.clc.uc.edu%252FFankhauser%252FLabs%252FMicrographs%252FLactobacillus_P7121141.jpg%26rurl=http%253A%252F%252Fbiology.clc.uc.edu%252FFankhauser%252FLabs%252FMicrographs%26size=9.0kB%26name=Lactobacillus_P7121141.jpg%26p=lactobacillus%26type=jpeg%26no=13%26tt=9,127%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq7yD21EuUsAf5GJzbkF;_ylu=X3oDMTBjdmNoOTVjBHBvcwMyBHNlYwNzcg--/SIG=1fqu7j9pq/EXP=1148084594/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlactobacillus%2526fr%253DFP-tab-img-t%2526toggle%253D1%2526cop%253D%2526ei%253DUTF-8%26w=288%26h=216%26imgurl=bioweb.usu.edu%252Fmicroscopy%252Flactobacillus%252520brevis.jpg%26rurl=http%253A%252F%252Fbioweb.usu.edu%252Fmicroscopy%252FResearch.htm%26size=18.3kB%26name=lactobacillus%20brevis.jpg%26p=lactobacillus%26type=jpeg%26no=2%26tt=9,127%26ei=UTF-8


19/87

Microbiology HTL

ERYSIPELOTHRIX

E. rhusiopathiae, the only species of thisgenus, is better known as a veterinary

pathogen than as a human pathogen. When cultured on blood agar or some other

nutrient medium, Erysipelothrix formsnotably large colonies.

This ubiquitous microbe has been found inmany farm animals such as pigs, horses, andturkeys.


20/87

Microbiology HTL

Occasionaly, though, it can infect a

human host and cause an inflammatoryskin disease, Erysipeloid.

Treatment usually consists of penicillinG, ampicillin, or cephalothin.

Most clinical strains have been found tobe resistant to the super-antibiotic,vancomycin.


21/87

Microbiology HTL
http://rds.yahoo.com/_ylt=A9G_RquNEG1ENc4AqY2JzbkF;_ylu=X3oDMTBjMzRvMDBnBHBvcwM5BHNlYwNzcg--/SIG=1f5stfupv/EXP=1148084749/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Derysipelothrix%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=591%26h=389%26imgurl=www.asta-tiho.de%252Fmibi%252FDiagnostik%252F47lismonSBl.jpg%26rurl=http%253A%252F%252Fwww.asta-tiho.de%252Fmibi%252FDiagnostik%252FEryList.html%26size=41.7kB%26name=47lismonSBl.jpg%26p=erysipelothrix%26type=jpeg%26no=9%26tt=75%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_RquNEG1ENc4Aq42JzbkF;_ylu=X3oDMTBkaWRnNHZyBHBvcwMxMQRzZWMDc3I-/SIG=1jakr9drs/EXP=1148084749/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Derysipelothrix%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=338%26h=279%26imgurl=www.mercksource.com%252Fppdocs%252Fus%252Fcommon%252Fdorlands%252Fdorland%252Fchapters%252Fimages%252Fw0146_e_52.jpg%26rurl=http%253A%252F%252Fwww.mercksource.com%252Fpp%252Fus%252Fcns%252Fcns_hl_dorlands.jspzQzpgzEzzSzppdocszSzuszSzcommonzSzdorlandszSzdorlandzSzdmd_e_14zPzhtm%26size=41.9kB%26name=w0146_e_52.jpg%26p=erysipelothrix%26type=jpeg%26no=11%26tt=75%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_RquNEG1ENc4Apo2JzbkF;_ylu=X3oDMTBjcDR2NTN2BHBvcwM2BHNlYwNzcg--/SIG=1fqrpv6n7/EXP=1148084749/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Derysipelothrix%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=593%26h=318%26imgurl=www.diagnostics.be%252Fproducts%252Fveterinary%252FVS002.3.jpg%26rurl=http%253A%252F%252Fwww.diagnostics.be%252Fproducts%252Fveterinary%252Felisa%252Fswine%252FVS004.htm%26size=13.6kB%26name=VS002.3.jpg%26p=erysipelothrix%26type=jpeg%26no=6%26tt=75%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_RquNEG1ENc4Aoo2JzbkF;_ylu=X3oDMTBjdmNoOTVjBHBvcwMyBHNlYwNzcg--/SIG=1du0l0c5t/EXP=1148084749/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Derysipelothrix%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=640%26h=480%26imgurl=www.msu.edu%252F%257Eramosjo%252Fv9074.jpg%26rurl=http%253A%252F%252Fwww.msu.edu%252F%257Eramosjo%252F9074.htm%26size=128.5kB%26name=v9074.jpg%26p=erysipelothrix%26type=jpeg%26no=2%26tt=75%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_RquNEG1ENc4Ao42JzbkF;_ylu=X3oDMTBjcXBoZjEwBHBvcwMzBHNlYwNzcg--/SIG=1l5hd1oap/EXP=1148084749/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Derysipelothrix%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=700%26h=466%26imgurl=medecinepharmacie.univ-fcomte.fr%252Fbacterio_web%252Fimg%252Fphototheque%252FExamens%252520microscopiques%252FBGP%252FErysipelothrix_rhusiopathiae_Culture.jpg%26rurl=http%253A%252F%252Fmedecinepharmacie.univ-fcomte.fr%252Fbacterio_web%252Fexa_microscopiques%252Fbgp%252FErysipelothrix_rhusiopathiae_Culture.htm%26size=74.1kB%26name=Erysipelothrix_rhusiopathiae_Culture.jpg%26p=erysipelothrix%26type=jpeg%26no=3%26tt=75%26ei=UTF-8


22/87

Microbiology HTL

LISTERIA:

Gram-positive rod which is not capable offorming endospores.

Although several species of this bacterium

exist, our discussion will focus only on thetwo species of human pathogenic significance:L. monocytogenes andL. ivanovii.

In particular,L. monocytogenes has beenimplicated in several food poisoningepidemics. This is a normal inhabitant of thegastrointestinal tract and of animal feces


23/87

Microbiology HTL

Infected suffers from vomiting, nausea, and

diarrhea.

Those at high risk include newborns, pregnantwomen and their fetuses, the elderly, and persons

lacking a healthy immune system.

The bacterium usually causes septicema andmeningitis in patients with supressed immune

function.

Causes Listeriosis, an inflammation of the brain.

Antibiotics are recommended for treatment of

infection because most strains ofListeria are

sensitive to ampicillin and gentamicin.


24/87

Microbiology HTL
http://rds.yahoo.com/_ylt=A9G_Rq9xEW1E5FQB1HOJzbkF;_ylu=X3oDMTBkYTNuNGk0BHBvcwMxMARzZWMDc3I-/SIG=1h1u8erfb/EXP=1148084977/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlisteria%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=200%26h=200%26imgurl=www.genomenewsnetwork.org%252Fgnn_images%252Fnews_content%252F07_01%252FListeria_genome_food%252Flisteria.jpg%26rurl=http%253A%252F%252Fwww.genomenewsnetwork.org%252Farticles%252F07_01%252FListeria_genome_food.shtml%26size=14.5kB%26name=listeria.jpg%26p=listeria%26type=jpeg%26no=10%26tt=8,565%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq9xEW1E5FQB0HOJzbkF;_ylu=X3oDMTBjcDR2NTN2BHBvcwM2BHNlYwNzcg--/SIG=1gk3j96o6/EXP=1148084977/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlisteria%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=250%26h=169%26imgurl=www.techno.msu.ac.th%252FFN%252Fecenter%252Fpathogens%252Fimages%252Fpig-listeria2.jpg%26rurl=http%253A%252F%252Fwww.techno.msu.ac.th%252FFN%252Fecenter%252Fpathogens%252Flisteria__monocytogenes.htm%26size=16.4kB%26name=pig-listeria2.jpg%26p=listeria%26type=jpeg%26no=6%26tt=8,565%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq9xEW1E5FQBznOJzbkF;_ylu=X3oDMTBjb3ZrYjNkBHBvcwM0BHNlYwNzcg--/SIG=1drh94hlq/EXP=1148084977/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlisteria%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=173%26h=114%26imgurl=www.bact.wisc.edu%252FBact330%252FListeria.TEM2.jpeg%26rurl=http%253A%252F%252Fwww.bact.wisc.edu%252FBact330%26size=21.1kB%26name=Listeria.TEM2.jpeg%26p=listeria%26type=jpeg%26no=4%26tt=8,565%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq9xEW1E5FQBzXOJzbkF;_ylu=X3oDMTBjcXBoZjEwBHBvcwMzBHNlYwNzcg--/SIG=1fts9k53p/EXP=1148084977/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlisteria%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=553%26h=434%26imgurl=www.foodscience.cornell.edu%252Fwiedmann%252FListeria%252520EM%252520Picture.JPG%26rurl=http%253A%252F%252Fwww.foodscience.cornell.edu%252Fwiedmann%252Fresearch.htm%26size=206.2kB%26name=Listeria%20EM%20Picture.JPG%26p=listeria%26type=jpeg%26no=3%26tt=8,565%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq9xEW1E5FQBzHOJzbkF;_ylu=X3oDMTBjdmNoOTVjBHBvcwMyBHNlYwNzcg--/SIG=1el5td43d/EXP=1148084977/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlisteria%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=300%26h=210%26imgurl=www.thebestlinks.com%252Fimages%252F0%252F05%252FListeria.jpg%26rurl=http%253A%252F%252Fwww.thebestlinks.com%252FListeria_monocytogenes.html%26size=28.5kB%26name=Listeria.jpg%26p=listeria%26type=jpeg%26no=2%26tt=8,565%26ei=UTF-8


25/87

Microbiology HTL

Bacillus : (1)

B. anthracis (anthrax

bacillus)

Gram (+) , aerobic,

central spore, motile,

non-pathogenic except

anthracis, thermophilic

ends are concave orsquare, forms long

chains, jointed bamboo

cultural char.:medusa head colonies

inverted fir tree in stab

Biochemical char.:

ferment dextrose

indole is not produced

gelatin is liquefied

Note: Find differences

with B. subtilis


26/87

Microbiology HTL
http://rds.yahoo.com/S=96062857/K=anthrax/v=2/SID=w/l=II/R=1/SS=p/OID=c8c49ee02f02bbac/SIG=1h006ngn3/EXP=1122534183/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Danthrax%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=335&w=480&imgcurl=www.medterms.com%2Fimages%2Fanthrax.jpg&imgurl=www.medterms.com%2Fimages%2Fanthrax.jpg&size=44.6kB&name=anthrax.jpg&rcurl=http%3A%2F%2Fwww.medterms.com%2Fscript%2Fmain%2Fart.asp%3Farticlekey%3D18821&rurl=http%3A%2F%2Fwww.medterms.com%2Fscript%2Fmain%2Fart.asp%3Farticlekey%3D18821&p=anthrax&type=jpeg&no=1&tt=39,354&ei=UTF-8http://rds.yahoo.com/S=96062857/K=anthrax/v=2/SID=w/l=II/R=2/SS=p/OID=b2d68f871be8cfd4/SIG=1hhi4unhr/EXP=1122534183/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Danthrax%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=492&w=445&imgcurl=www.arches.uga.edu%2F%7Emfield%2Fanthrax_weeping_skin.jpg&imgurl=www.arches.uga.edu%2F%7Emfield%2Fanthrax_weeping_skin.jpg&size=53.4kB&name=anthrax_weeping_skin.jpg&rcurl=http%3A%2F%2Fwww.arches.uga.edu%2F%7Emfield%2Fbackground.htm&rurl=http%3A%2F%2Fwww.arches.uga.edu%2F%7Emfield%2Fbackground.htm&p=anthrax&type=jpeg&no=2&tt=39,354&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacillus+anthracis/v=2/SID=w/l=II/R=7/SS=p/OID=075c022220b4932a/SIG=1lq2io5g2/EXP=1122534134/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Banthracis%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-4075278827_q-YFR5hiz.a7ShVQrHNVj.GQABAA%40%40&h=399&w=496&imgcurl=www.uvp5.univ-paris5.fr%2FMICROBES%2FDiagnostic%2FBacillus%2FB2.jpg&imgurl=www.uvp5.univ-paris5.fr%2FMICROBES%2FDiagnostic%2FBacillus%2FB2.jpg&size=52.1kB&name=B2.jpg&rcurl=http%3A%2F%2Fwww.uvp5.univ-paris5.fr%2FMICROBES%2FDiagnostic%2FBacillus%2FBacillus.asp&rurl=http%3A%2F%2Fwww.uvp5.univ-paris5.fr%2FMICROBES%2FDiagnostic%2FBacillus%2FBacillus.asp&p=bacillus+anthracis&type=jpeg&no=7&tt=892&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacillus+anthracis/v=2/SID=w/l=II/R=5/SS=p/OID=7a7b60caf1d79878/SIG=1odfd4mpq/EXP=1122534134/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Banthracis%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-4075278827_q-YFR5hiz.a7ShVQrHNVj.GQABAA%40%40&h=298&w=250&imgcurl=www.lbl.gov%2FScience-Articles%2FArchive%2Fassets%2Fimages%2F2004%2FMar-24%2FBacillus_anthracis.jpg&imgurl=www.lbl.gov%2FScience-Articles%2FArchive%2Fassets%2Fimages%2F2004%2FMar-24%2FBacillus_anthracis.jpg&size=119.6kB&name=Bacillus_anthracis.jpg&rcurl=http%3A%2F%2Fwww.lbl.gov%2FScience-Articles%2FArchive%2Fsb-ESD-cataloging-bacteria.html&rurl=http%3A%2F%2Fwww.lbl.gov%2FScience-Articles%2FArchive%2Fsb-ESD-cataloging-bacteria.html&p=bacillus+anthracis&type=jpeg&no=5&tt=892&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacilus+anthracis/v=2/SID=w/l=II/R=3/SS=p/OID=6ea02c1f8252b52a/SIG=1l73q5dfj/EXP=1122534101/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacilus%2Banthracis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=500&w=400&imgcurl=www.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fobr%2Fbacilus_anthracis_3.jpg&imgurl=www.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fobr%2Fbacilus_anthracis_3.jpg&size=34.2kB&name=bacilus_anthracis_3.jpg&rcurl=http%3A%2F%2Fwww.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fobr%2Fbacillus_anthracis_3.htm&rurl=http%3A%2F%2Fwww.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fobr%2Fbacillus_anthracis_3.htm&p=bacilus+anthracis&type=jpeg&no=3&tt=5&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacilus+anthracis/v=2/SID=w/l=II/R=2/SS=p/OID=4c9d74bb707c4252/SIG=1hgkhsnvg/EXP=1122534101/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacilus%2Banthracis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=225&w=220&imgcurl=www.euuzlet.hu%2Fillusztracio%2Fbacilusanthracis.jpg&imgurl=www.euuzlet.hu%2Fillusztracio%2Fbacilusanthracis.jpg&size=15.2kB&name=bacilusanthracis.jpg&rcurl=http%3A%2F%2Fwww.euuzlet.hu%2Fszines%2F2001%2Foktober.html&rurl=http%3A%2F%2Fwww.euuzlet.hu%2Fszines%2F2001%2Foktober.html&p=bacilus+anthracis&type=jpeg&no=2&tt=5&ei=UTF-8


27/87

Microbiology HTL

Bacillus : (2)Anthrax - lesion like

carbuncle, large ,

black, necrotic

death due tomechanical clogging

of capillaries

Types:Bacteremia -

blood

Cutaneous - skin

Pulmonary - woolsorters disease

Intestinal

Specimen - blood,swab w/ pus,

sputum, feces

Diagnosis:direct microscopic

culture

animal inoculation


28/87

Microbiology HTL
http://rds.yahoo.com/S=96062857/K=bacillus+subtilis/v=2/SID=w/l=II/R=10/SS=p/OID=c2762cebce330678/SIG=1in233pco/EXP=1122534232/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Bsubtilis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=116&w=177&imgcurl=nengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Fbacillus-subtilis.jpg&imgurl=nengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Fbacillus-subtilis.jpg&size=4.0kB&name=bacillus-subtilis.jpg&rcurl=http%3A%2F%2Fnengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Ffm04-5.htm&rurl=http%3A%2F%2Fnengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Ffm04-5.htm&p=bacillus+subtilis&type=jpeg&no=10&tt=611&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacillus+subtilis/v=2/SID=w/l=II/R=7/SS=p/OID=e275f71aa351ac52/SIG=1i11nss4r/EXP=1122534232/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Bsubtilis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=194&w=295&imgcurl=www.cgr.ki.se%2Fcgr%2Fpersons%2Fraf%2Fimages%2Fbacillus.gif&imgurl=www.cgr.ki.se%2Fcgr%2Fpersons%2Fraf%2Fimages%2Fbacillus.gif&size=55.2kB&name=bacillus.gif&rcurl=http%3A%2F%2Fwww.cgb.ki.se%2Fcgr%2Fpersons%2Fraf%2Fgenomes.html&rurl=http%3A%2F%2Fwww.cgb.ki.se%2Fcgr%2Fpersons%2Fraf%2Fgenomes.html&p=bacillus+subtilis&type=gif&no=7&tt=611&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacillus+subtilis/v=2/SID=w/l=II/R=2/SS=p/OID=dc1e81806e621e8e/SIG=1htq3a7f9/EXP=1122534232/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Bsubtilis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=306&w=274&imgcurl=www2.austincc.edu%2Fmicrobugz%2Fimages%2F03bsubtilis.jpg&imgurl=www2.austincc.edu%2Fmicrobugz%2Fimages%2F03bsubtilis.jpg&size=19.8kB&name=03bsubtilis.jpg&rcurl=http%3A%2F%2Fwww2.austincc.edu%2Fmicrobugz%2F03morphology.html&rurl=http%3A%2F%2Fwww2.austincc.edu%2Fmicrobugz%2F03morphology.html&p=bacillus+subtilis&type=jpeg&no=2&tt=611&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacillus+subtilis/v=2/SID=w/l=II/R=1/SS=p/OID=08e2c2ccaa0d19c2/SIG=1klf80tdi/EXP=1122534232/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Bsubtilis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=253&w=300&imgcurl=www.iccb.state.il.us%2Fpt3%2Fimages%2Fsci%2Fmod11%2Fbacillus_subtilis.jpg&imgurl=www.iccb.state.il.us%2Fpt3%2Fimages%2Fsci%2Fmod11%2Fbacillus_subtilis.jpg&size=12.6kB&name=bacillus_subtilis.jpg&rcurl=http%3A%2F%2Fwww.iccb.state.il.us%2Fpt3%2Fmod%2Fscience%2Fmod_bio111%2Fmod10%2Fp4.html&rurl=http%3A%2F%2Fwww.iccb.state.il.us%2Fpt3%2Fmod%2Fscience%2Fmod_bio111%2Fmod10%2Fp4.html&p=bacillus+subtilis&type=jpeg&no=1&tt=611&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacillus+subtilis/v=2/SID=w/l=II/R=4/SS=p/OID=4464914072a430b4/SIG=1jhocub4d/EXP=1122534232/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Bsubtilis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=142&w=165&imgcurl=www.arvanitakis.com%2Fgr%2Fc%2Fbac_gal%2Fbcillus_subtitlis_eu.jpg&imgurl=www.arvanitakis.com%2Fgr%2Fc%2Fbac_gal%2Fbcillus_subtitlis_eu.jpg&size=8.2kB&name=bcillus_subtitlis_eu.jpg&rcurl=http%3A%2F%2Fwww.arvanitakis.com%2Fgr%2Fc%2Fbac_gal%2Fbacillus_subtilis.htm&rurl=http%3A%2F%2Fwww.arvanitakis.com%2Fgr%2Fc%2Fbac_gal%2Fbacillus_subtilis.htm&p=bacillus+subtilis&type=jpeg&no=4&tt=611&ei=UTF-8


29/87

Microbiology HTL

Clostridium : (1)

C. tetani (tetanus ordrumstick bacillus)

motile

medium in length terminal spore

Thioglycolate broth

pathogenesis: fever,pain , Lockjaw,

sardonic smile,

spasm

Diagnosis:

culture

secretion fromlesion - BAP,

Thioglycolate

Prevention:active immunity

with toxoid


30/87

Microbiology HTL
http://rds.yahoo.com/S=96062857/K=clostridium+tetani+%26+tetanus/v=2/SID=w/l=II/R=5/SS=p/OID=32262fab64339fa8/SIG=1ht3mcc41/EXP=1122534324/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Btetani%2B%2526%2Btetanus%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=173&w=433&imgcurl=www.bact.wisc.edu%2FBact330%2Fclostridium.jpeg&imgurl=www.bact.wisc.edu%2FBact330%2Fclostridium.jpeg&size=59.2kB&name=clostridium.jpeg&rcurl=http%3A%2F%2Fwww.bact.wisc.edu%2FBact330%2Flecturetetbot&rurl=http%3A%2F%2Fwww.bact.wisc.edu%2FBact330%2Flecturetetbot&p=clostridium+tetani+%26+tetanus&type=jpeg&no=5&tt=37&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+tetani+%26+tetanus/v=2/SID=w/l=II/R=17/SS=p/OID=bf795e712e414354/SIG=1kvj5rk2h/EXP=1122534324/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Btetani%2B%2526%2Btetanus%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=204&w=260&imgcurl=chemweb.calpoly.edu%2Fchem%2Fbailey%2F377%2FPagesW02%2FKristenS%2Fctetani.jpg&imgurl=chemweb.calpoly.edu%2Fchem%2Fbailey%2F377%2FPagesW02%2FKristenS%2Fctetani.jpg&size=15.8kB&name=ctetani.jpg&rcurl=http%3A%2F%2Fchemweb.calpoly.edu%2Fchem%2Fbailey%2F377%2FPagesW02%2FKristenS&rurl=http%3A%2F%2Fchemweb.calpoly.edu%2Fchem%2Fbailey%2F377%2FPagesW02%2FKristenS&p=clostridium+tetani+%26+tetanus&type=jpeg&no=17&tt=37&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+tetani+%26+tetanus/v=2/SID=w/l=II/R=16/SS=p/OID=40750e874a2f54c6/SIG=1jl38ngpm/EXP=1122534324/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Btetani%2B%2526%2Btetanus%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=98&w=120&imgcurl=www.aposchinkenplatz.de%2Finformation%2Fimpfen%2Ftetanus.jpg&imgurl=www.aposchinkenplatz.de%2Finformation%2Fimpfen%2Ftetanus.jpg&size=4.2kB&name=tetanus.jpg&rcurl=http%3A%2F%2Fwww.aposchinkenplatz.de%2Finformation%2Fimpfen%2Ftetanus.htm&rurl=http%3A%2F%2Fwww.aposchinkenplatz.de%2Finformation%2Fimpfen%2Ftetanus.htm&p=clostridium+tetani+%26+tetanus&type=jpeg&no=16&tt=37&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+tetani+%26+tetanus/v=2/SID=w/l=II/R=14/SS=p/OID=04c50f3453205318/SIG=1h484inba/EXP=1122534324/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Btetani%2B%2526%2Btetanus%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=126&w=235&imgcurl=www.pathguy.com%2Flectures%2Ftetanus.jpg&imgurl=www.pathguy.com%2Flectures%2Ftetanus.jpg&size=5.8kB&name=tetanus.jpg&rcurl=http%3A%2F%2Fwww.pathguy.com%2Flectures%2Finfect.htm&rurl=http%3A%2F%2Fwww.pathguy.com%2Flectures%2Finfect.htm&p=clostridium+tetani+%26+tetanus&type=jpeg&no=14&tt=37&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+tetani+%26+tetanus/v=2/SID=w/l=II/R=1/SS=p/OID=d3768a7c68b55948/SIG=1kcb0npe6/EXP=1122534324/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Btetani%2B%2526%2Btetanus%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=102&w=154&imgcurl=www.lek.sk%2Fimg%2Fpictures%2Fgaleria_mikroorganizmy%2Fclostridium_tetani.jpg&imgurl=www.lek.sk%2Fimg%2Fpictures%2Fgaleria_mikroorganizmy%2Fclostridium_tetani.jpg&size=3.5kB&name=clostridium_tetani.jpg&rcurl=http%3A%2F%2Fwww.lek.sk%2Ftemplate.php%3Fidms%3D14%26locate%3D&rurl=http%3A%2F%2Fwww.lek.sk%2Ftemplate.php%3Fidms%3D14%26locate%3D&p=clostridium+tetani+%26+tetanus&type=jpeg&no=1&tt=37&ei=UTF-8


31/87

Microbiology HTL

Clostridium: (2)

C. botulinum (Von

Ermengens bacilli)

motile long

subterminal spore

Culture:

Chopped meat

BA medium

Pathogenesis:

food intoxication

food infection

food poisoning

Culture of leftover

food or stool

History of patient


32/87

Microbiology HTL
http://rds.yahoo.com/S=96062857/K=clostridium+botulinum/v=2/SID=w/l=II/R=18/SS=p/OID=434228dc5519fc64/SIG=1lbrfmor0/EXP=1122534420/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bbotulinum%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=346&w=182&imgcurl=www.cvm.tamu.edu%2Fheb%2Farchive%2F9899%2FProg5%2FImages%2Fclostridium%2520botulinum.GIF&imgurl=www.cvm.tamu.edu%2Fheb%2Farchive%2F9899%2FProg5%2FImages%2Fclostridium%2520botulinum.GIF&size=32.3kB&name=clostridium%20botulinum.GIF&rcurl=http%3A%2F%2Fwww.cvm.tamu.edu%2Fheb%2Farchive%2F9899%2FProg5%2Fstoring.html&rurl=http%3A%2F%2Fwww.cvm.tamu.edu%2Fheb%2Farchive%2F9899%2FProg5%2Fstoring.html&p=clostridium+botulinum&type=gif&no=18&tt=434&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+botulinum/v=2/SID=w/l=II/R=19/SS=p/OID=b271ad352f70488e/SIG=1gkquvmj6/EXP=1122534420/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bbotulinum%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=1002&w=750&imgcurl=www.zuova.cz%2Fnrl%2Fpic%2Fnrlpab23_clbo1.jpg&imgurl=www.zuova.cz%2Fnrl%2Fpic%2Fnrlpab23_clbo1.jpg&size=30.2kB&name=nrlpab23_clbo1.jpg&rcurl=http%3A%2F%2Fwww.zuova.cz%2Fnrl%2Fnrlpab23.php&rurl=http%3A%2F%2Fwww.zuova.cz%2Fnrl%2Fnrlpab23.php&p=clostridium+botulinum&type=jpeg&no=19&tt=434&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+botulinum/v=2/SID=w/l=II/R=4/SS=p/OID=a8de24202a176b80/SIG=1g1qrch12/EXP=1122534420/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bbotulinum%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=342&w=341&imgcurl=www.vidyya.com%2F6images%2FbotoxZ.jpg&imgurl=www.vidyya.com%2F6images%2FbotoxZ.jpg&size=102.3kB&name=botoxZ.jpg&rcurl=http%3A%2F%2Fwww.vidyya.com%2Fvol6%2Fv6i348_2.htm&rurl=http%3A%2F%2Fwww.vidyya.com%2Fvol6%2Fv6i348_2.htm&p=clostridium+botulinum&type=jpeg&no=4&tt=434&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+botulinum/v=2/SID=w/l=II/R=8/SS=p/OID=5c43b22fec41b18c/SIG=1nuuqaco2/EXP=1122534420/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bbotulinum%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=220&w=170&imgcurl=biology.kenyon.edu%2FMicrobial_Biorealm%2Fbacteria%2Fgram-positive%2Fclostridium%2Fbotulism_160.jpg&imgurl=biology.kenyon.edu%2FMicrobial_Biorealm%2Fbacteria%2Fgram-positive%2Fclostridium%2Fbotulism_160.jpg&size=17.3kB&name=botulism_160.jpg&rcurl=http%3A%2F%2Fbiology.kenyon.edu%2FMicrobial_Biorealm%2Fbacteria%2Fgram-positive%2Fclostridium%2Fclostridium.htm&rurl=http%3A%2F%2Fbiology.kenyon.edu%2FMicrobial_Biorealm%2Fbacteria%2Fgram-positive%2Fclostridium%2Fclostridium.htm&p=clostridium+botulinum&type=jpeg&no=8&tt=434&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+botulinum/v=2/SID=w/l=II/R=2/SS=p/OID=9d58162f41cf0228/SIG=1htg6ah2t/EXP=1122534420/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bbotulinum%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=100&w=100&imgcurl=www.vcell.de%2Fpics%2Fclostridium_botulinum-.jpg&imgurl=www.vcell.de%2Fpics%2Fclostridium_botulinum-.jpg&size=6.5kB&name=clostridium_botulinum-.jpg&rcurl=http%3A%2F%2Fwww.vcell.de%2Fgesundheitspark%2Fbotulismus.html&rurl=http%3A%2F%2Fwww.vcell.de%2Fgesundheitspark%2Fbotulismus.html&p=clostridium+botulinum&type=jpeg&no=2&tt=434&ei=UTF-8


33/87

Microbiology HTL

Clostridium: (3)

C. perfringens (C.welchii,

Frankels or gas

gangrene)

non-motile

short

central spore

Culture media:

same as C. botulinum

pathogenesis

gas gangrene

food infection

Diagnosis:

swab from lesion

& culture

microscopic exam

culture of pus


34/87

Microbiology HTL

Clostridium: (4)

Clostridium difficile:

Causes

pseudomembranecolitis

Relatively resistant to

most antibiotics

Produces cytopathic

toxins and enterotoxin

Diarrhea, to marked

diarrhea to necrosis of

mucosa

Diagnosis

Demonstrate

neutralizeable toxin in

stoolDetecting enterotoxin

by immunoassay


35/87

Microbiology HTL
http://rds.yahoo.com/S=96062857/K=clostridium+perfringens/v=2/SID=w/l=II/R=15/SS=p/OID=91f8318dba62e66c/SIG=1op1s6kjk/EXP=1122534513/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bperfringens%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=494&w=961&imgcurl=www.vets-schoeppingen.de%2Fhtml%2Fschweine%2FClostridium%2520perfringens%2FImage8.gif&imgurl=www.vets-schoeppingen.de%2Fhtml%2Fschweine%2FClostridium%2520perfringens%2FImage8.gif&size=150.7kB&name=Image8.gif&rcurl=http%3A%2F%2Fwww.vets-schoeppingen.de%2Fhtml%2Fschweine%2FClostridium%2520perfringens%2FClostridien%2520perfringens%2520beim%2520Ferkel.htm&rurl=http%3A%2F%2Fwww.vets-schoeppingen.de%2Fhtml%2Fschweine%2FClostridium%2520perfringens%2FClostridien%2520perfringens%2520beim%2520Ferkel.htm&p=clostridium+perfringens&type=gif&no=15&tt=323&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+perfringens/v=2/SID=w/l=II/R=14/SS=p/OID=32be41d9c417ca80/SIG=1lle81q2f/EXP=1122534513/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bperfringens%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=360&w=224&imgcurl=www.cvm.tamu.edu%2Fheb%2Farchive%2F9899%2FProg5%2FImages%2Fclostridium%2520perfringens.GIF&imgurl=www.cvm.tamu.edu%2Fheb%2Farchive%2F9899%2FProg5%2FImages%2Fclostridium%2520perfringens.GIF&size=33.6kB&name=clostridium%20perfringens.GIF&rcurl=http%3A%2F%2Fwww.cvm.tamu.edu%2Fheb%2Farchive%2F9899%2FProg5%2Fcooling.html&rurl=http%3A%2F%2Fwww.cvm.tamu.edu%2Fheb%2Farchive%2F9899%2FProg5%2Fcooling.html&p=clostridium+perfringens&type=gif&no=14&tt=323&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+perfringens/v=2/SID=w/l=II/R=5/SS=p/OID=7cda92fca7a9d4c6/SIG=1hheds82s/EXP=1122534513/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bperfringens%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=207&w=188&imgcurl=www.bact.wisc.edu%2FBact330%2FnfC.perfringens4.jpeg&imgurl=www.bact.wisc.edu%2FBact330%2FnfC.perfringens4.jpeg&size=38.6kB&name=nfC.perfringens4.jpeg&rcurl=http%3A%2F%2Fwww.bact.wisc.edu%2FBact330%2Flecturenf&rurl=http%3A%2F%2Fwww.bact.wisc.edu%2FBact330%2Flecturenf&p=clostridium+perfringens&type=jpeg&no=5&tt=323&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+perfringens/v=2/SID=w/l=II/R=3/SS=p/OID=5a3350413f670e0a/SIG=1ji1r3nhf/EXP=1122534513/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bperfringens%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=160&w=200&imgcurl=nengjung.kit.ac.kr%2F%7Eckisu%2Ffs%2F05%2Fclostridium-perfringens.jpg&imgurl=nengjung.kit.ac.kr%2F%7Eckisu%2Ffs%2F05%2Fclostridium-perfringens.jpg&size=5.4kB&name=clostridium-perfringens.jpg&rcurl=http%3A%2F%2Fnengjung.kit.ac.kr%2F%7Eckisu%2Ffs%2F05%2F05-04.htm&rurl=http%3A%2F%2Fnengjung.kit.ac.kr%2F%7Eckisu%2Ffs%2F05%2F05-04.htm&p=clostridium+perfringens&type=jpeg&no=3&tt=323&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+perfringens/v=2/SID=w/l=II/R=2/SS=p/OID=9362949b8fb09ab4/SIG=1jkhia333/EXP=1122534513/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bperfringens%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=113&w=149&imgcurl=nengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Fclostridium-perfringens.jpg&imgurl=nengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Fclostridium-perfringens.jpg&size=4.1kB&name=clostridium-perfringens.jpg&rcurl=http%3A%2F%2Fnengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Ffm04-5.htm&rurl=http%3A%2F%2Fnengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Ffm04-5.htm&p=clostridium+perfringens&type=jpeg&no=2&tt=323&ei=UTF-8


36/87

Microbiology HTL

Actinomyces israelii

Causes actinomycosis

Vary considerably in length

Maybe branched or un branched

Grows slowly, on agar some strains produce

colonies similar to molar teeth

Sometimes confused with corynebacterium

Sensitive to penicillin & erythromycin


37/87

Microbiology HTL

Propionibacterium acne:

Normal flora of the skin but can cause

disease when they infect shunt and

appliancesOn gram stain, highly pleomorphic

Participates in the genesis of acne

May contaminate blood and CSF once itpenetrates the skin


38/87

Microbiology HTL

Eubacterium, Bifidobacterium &

Arachnia:These 3 genera are made up of anaerobic,

pleomorphic, gram positive rods

There are several speciesAre found in mixed infections associated

with oropharyngeal or bowel flora


39/87

Microbiology HTL

Gram - Bacilli

Enteric Bacteria

(Enterobacter family) found

in intestines of man &animals, parasitic &

saprophytic, mostly

pathogenic

causes intestinal diseases &

septicemic infections

produce Hydrogen sulfide

Media:

Selenite broth

Tetrathionate broth

Peptone

Tryptone


40/87

Microbiology HTL
http://rds.yahoo.com/S=96062857/K=enterobacter/v=2/SID=w/l=II/R=20/SS=p/OID=d87696179923d552/SIG=1hc1kh146/EXP=1122534790/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Denterobacter%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=619&w=800&imgcurl=conganat.uninet.edu%2Fautores%2Ftrabajos%2FT068%2FI002.JPG&imgurl=conganat.uninet.edu%2Fautores%2Ftrabajos%2FT068%2FI002.JPG&size=126.8kB&name=I002.JPG&rcurl=http%3A%2F%2Fconganat.uninet.edu%2Fautores%2Ftrabajos%2FT068&rurl=http%3A%2F%2Fconganat.uninet.edu%2Fautores%2Ftrabajos%2FT068&p=enterobacter&type=jpeg&no=20&tt=252&ei=UTF-8http://rds.yahoo.com/S=96062857/K=enterobacter/v=2/SID=w/l=II/R=19/SS=p/OID=e6cadd014c61ade6/SIG=1hchprmqd/EXP=1122534790/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Denterobacter%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=607&w=800&imgcurl=conganat.uninet.edu%2Fautores%2Ftrabajos%2FT068%2FI001.JPG&imgurl=conganat.uninet.edu%2Fautores%2Ftrabajos%2FT068%2FI001.JPG&size=108.3kB&name=I001.JPG&rcurl=http%3A%2F%2Fconganat.uninet.edu%2Fautores%2Ftrabajos%2FT068&rurl=http%3A%2F%2Fconganat.uninet.edu%2Fautores%2Ftrabajos%2FT068&p=enterobacter&type=jpeg&no=19&tt=252&ei=UTF-8http://rds.yahoo.com/S=96062857/K=enterobacter/v=2/SID=w/l=II/R=1/SS=p/OID=46c223c312bb37e4/SIG=1g3on3t93/EXP=1122534790/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Denterobacter%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=304&w=340&imgcurl=anka.livstek.lth.se%3A2080%2Fe-aer61.jpg&imgurl=anka.livstek.lth.se%3A2080%2Fe-aer61.jpg&size=36.6kB&name=e-aer61.jpg&rcurl=http%3A%2F%2Fanka.livstek.lth.se%3A2080%2FE_aerogenes.htm&rurl=http%3A%2F%2Fanka.livstek.lth.se%3A2080%2FE_aerogenes.htm&p=enterobacter&type=jpeg&no=1&tt=252&ei=UTF-8http://rds.yahoo.com/S=96062857/K=enterobacter/v=2/SID=w/l=II/R=2/SS=p/OID=99ebe730be7f5276/SIG=1ibtb1srl/EXP=1122534790/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Denterobacter%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=230&w=350&imgcurl=www.visualsunlimited.com%2Fimages%2Fwatermarked%2F673%2F67319.jpg&imgurl=www.visualsunlimited.com%2Fimages%2Fwatermarked%2F673%2F67319.jpg&size=14.5kB&name=67319.jpg&rcurl=http%3A%2F%2Fwww.visualsunlimited.com%2Fbrowse%2Fvu673%2Fvu67319.html&rurl=http%3A%2F%2Fwww.visualsunlimited.com%2Fbrowse%2Fvu673%2Fvu67319.html&p=enterobacter&type=jpeg&no=2&tt=252&ei=UTF-8http://rds.yahoo.com/S=96062857/K=enterobacter/v=2/SID=w/l=II/R=6/SS=p/OID=46b0c92d91f2cf00/SIG=1lfk1vc3v/EXP=1122534790/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Denterobacter%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=772&w=580&imgcurl=www.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2Fgraphics%2F347enterobacter1.jpg&imgurl=www.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2Fgraphics%2F347enterobacter1.jpg&size=120.6kB&name=347enterobacter1.jpg&rcurl=http%3A%2F%2Fwww.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2F60_347.htm&rurl=http%3A%2F%2Fwww.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2F60_347.htm&p=enterobacter&type=jpeg&no=6&tt=252&ei=UTF-8


41/87

Microbiology HTL

Gram (-) bacilli

Plating media:

Desoxycholate,

CitrateSSA, EMB, BAP

McConkey,

Brilliant green

Indicators:

Neutral red:

Red/colorlessPhenol red:

yellow/red

Andrades: pink/colorless


42/87

Microbiology HTL

Escherichia

Escherichia coli

found in the normal

flora of GIT, outsidethe GIT it causes

cystitis, peritonitis

index for food & water

pollution IMViC - ++--

A.aerogenes - --++
http://rds.yahoo.com/S=96062857/K=escherichia+coli/v=2/SID=w/l=II/R=3/SS=p/OID=4244d8c63d6c8a70/SIG=1kkcle5ud/EXP=1122534878/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Descherichia%2Bcoli%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=200&w=250&imgcurl=www.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fobr%2Fescherichia_coli_1s.jpg&imgurl=www.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fobr%2Fescherichia_coli_1s.jpg&size=7.8kB&name=escherichia_coli_1s.jpg&rcurl=http%3A%2F%2Fwww.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fescherichia_coli.htm&rurl=http%3A%2F%2Fwww.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fescherichia_coli.htm&p=escherichia+coli&type=jpeg&no=3&tt=4,399&ei=UTF-8http://rds.yahoo.com/S=96062857/K=escherichia+coli/v=2/SID=w/l=II/R=8/SS=p/OID=711d48c1661fee08/SIG=1i1ef2tmt/EXP=1122534878/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Descherichia%2Bcoli%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=182&w=150&imgcurl=tagc.univ-mrs.fr%2FBioInteractif%2Fpub%2F_e-colism.jpg&imgurl=tagc.univ-mrs.fr%2FBioInteractif%2Fpub%2F_e-colism.jpg&size=25.1kB&name=_e-colism.jpg&rcurl=http%3A%2F%2Ftagc.univ-mrs.fr%2FBioInteractif%2Fpub%2Fpub_pole.html&rurl=http%3A%2F%2Ftagc.univ-mrs.fr%2FBioInteractif%2Fpub%2Fpub_pole.html&p=escherichia+coli&type=jpeg&no=8&tt=4,399&ei=UTF-8http://rds.yahoo.com/S=96062857/K=escherichia+coli/v=2/SID=w/l=II/R=4/SS=p/OID=183ce63581708200/SIG=1iflcgsu7/EXP=1122534878/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Descherichia%2Bcoli%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=398&w=400&imgcurl=res2.agr.ca%2FLethbridge%2Femia%2Fimages%2FSEMproj%2FEcoli.jpg&imgurl=res2.agr.ca%2FLethbridge%2Femia%2Fimages%2FSEMproj%2FEcoli.jpg&size=59.4kB&name=Ecoli.jpg&rcurl=http%3A%2F%2Fres2.agr.ca%2FLethbridge%2Femia%2FSEMproj%2Fecoli_e.htm&rurl=http%3A%2F%2Fres2.agr.ca%2FLethbridge%2Femia%2FSEMproj%2Fecoli_e.htm&p=escherichia+coli&type=jpeg&no=4&tt=4,399&ei=UTF-8


43/87

Microbiology HTL

Qualitative analysis of

water:

Organolytic - physical

Presumptive - lactose

broth for gas

Confirmatory - EMB

Completed - RDS,

TSI, KIA
http://rds.yahoo.com/S=96062857/K=escherichia+coli/v=2/SID=w/l=II/R=5/SS=p/OID=9ce15ab2acad45a4/SIG=1gjirtmom/EXP=1122534878/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Descherichia%2Bcoli%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=256&w=384&imgcurl=www.historique.net%2Fmicrobes%2Fimages%2Fgas.jpg&imgurl=www.historique.net%2Fmicrobes%2Fimages%2Fgas.jpg&size=24.8kB&name=gas.jpg&rcurl=http%3A%2F%2Fwww.historique.net%2Fmicrobes%2Fgas.html&rurl=http%3A%2F%2Fwww.historique.net%2Fmicrobes%2Fgas.html&p=escherichia+coli&type=jpeg&no=5&tt=4,399&ei=UTF-8http://rds.yahoo.com/S=96062857/K=escherichia+coli/v=2/SID=w/l=II/R=4/SS=p/OID=183ce63581708200/SIG=1iflcgsu7/EXP=1122534878/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Descherichia%2Bcoli%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=398&w=400&imgcurl=res2.agr.ca%2FLethbridge%2Femia%2Fimages%2FSEMproj%2FEcoli.jpg&imgurl=res2.agr.ca%2FLethbridge%2Femia%2Fimages%2FSEMproj%2FEcoli.jpg&size=59.4kB&name=Ecoli.jpg&rcurl=http%3A%2F%2Fres2.agr.ca%2FLethbridge%2Femia%2FSEMproj%2Fecoli_e.htm&rurl=http%3A%2F%2Fres2.agr.ca%2FLethbridge%2Femia%2FSEMproj%2Fecoli_e.htm&p=escherichia+coli&type=jpeg&no=4&tt=4,399&ei=UTF-8http://rds.yahoo.com/S=96062857/K=escherichia+coli/v=2/SID=w/l=II/R=1/SS=p/OID=f3e2f826540590fe/SIG=1g989a6a8/EXP=1122534878/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Descherichia%2Bcoli%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=393&w=500&imgcurl=www.siue.edu%2F%7Ecbwilso%2FEcoli.jpg&imgurl=www.siue.edu%2F%7Ecbwilso%2FEcoli.jpg&size=36.3kB&name=Ecoli.jpg&rcurl=http%3A%2F%2Fwww.siue.edu%2F%7Ecbwilso%2F250graphics00.htm&rurl=http%3A%2F%2Fwww.siue.edu%2F%7Ecbwilso%2F250graphics00.htm&p=escherichia+coli&type=jpeg&no=1&tt=4,399&ei=UTF-8


44/87

Microbiology HTL

Salmonella Salmonella typhosa

circular, small,

smooth surface w/

even edge colonies Indole (-), Citrate

(+), no lactose

fermentation,

ferments glucose,

maltose
http://rds.yahoo.com/S=96062857/K=salmonella+typhi/v=2/SID=w/l=II/R=1/SS=p/OID=b1cc0d89803d81ca/SIG=1mocgfhsp/EXP=1122535007/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Btyphi%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-2481628037_q-X64O6BvAYaSHj.HChHAX7AABAA%40%40&h=490&w=591&imgcurl=www.gastrohygiene.li%2Fmikroorganismen%2Fsalmonella%2520typhi%252010cm.jpg&imgurl=www.gastrohygiene.li%2Fmikroorganismen%2Fsalmonella%2520typhi%252010cm.jpg&size=100.9kB&name=salmonella%20typhi%2010cm.jpg&rcurl=http%3A%2F%2Fwww.gastrohygiene.li%2Fmikroorganismen%2Fsalmonella_typhi_zoom_page.htm&rurl=http%3A%2F%2Fwww.gastrohygiene.li%2Fmikroorganismen%2Fsalmonella_typhi_zoom_page.htm&p=salmonella+typhi&type=jpeg&no=1&tt=172&ei=UTF-8http://rds.yahoo.com/S=96062857/K=salmonella+typhi/v=2/SID=w/l=II/R=5/SS=p/OID=332c044960cba4b8/SIG=1kt4inpg7/EXP=1122535007/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Btyphi%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-2481628037_q-X64O6BvAYaSHj.HChHAX7AABAA%40%40&h=222&w=160&imgcurl=fr.wikipedia.org%2Fupload%2F8%2F8c%2FSalmonella_enterica.jpg&imgurl=fr.wikipedia.org%2Fupload%2F8%2F8c%2FSalmonella_enterica.jpg&size=48.9kB&name=Salmonella_enterica.jpg&rcurl=http%3A%2F%2Ffr.wikipedia.org%2Fwiki%2FFi%25C3%25A8vre_typho%25C3%25AFde&rurl=http%3A%2F%2Ffr.wikipedia.org%2Fwiki%2FFi%25C3%25A8vre_typho%25C3%25AFde&p=salmonella+typhi&type=jpeg&no=5&tt=172&ei=UTF-8


45/87

Microbiology HTL

Antigenic Structure:H-flagellar

O-somatic

Vi-capsular

Widals Test:

^titer O - active

infection

^titer H - postvaccination

^Vi -carrier
http://rds.yahoo.com/S=96062857/K=salmonella+typhi/v=2/SID=w/l=II/R=9/SS=p/OID=f6c7066c85bcf18e/SIG=1nv19n472/EXP=1122535007/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Btyphi%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-2481628037_q-X64O6BvAYaSHj.HChHAX7AABAA%40%40&h=173&w=200&imgcurl=www.ecplanet.com%2Fpic%2F2003%2F01%2F1042201189%2Fsalmonella_typhimurium.jpg&imgurl=www.ecplanet.com%2Fpic%2F2003%2F01%2F1042201189%2Fsalmonella_typhimurium.jpg&size=6.2kB&name=salmonella_typhimurium.jpg&rcurl=http%3A%2F%2Fwww3.ecplanet.com%2Fcanale%2Fsalute-7%2Fpatologie-68%2F0%2F0%2F4900%2Fit%2Fchiasso_line.xml&rurl=http%3A%2F%2Fwww3.ecplanet.com%2Fcanale%2Fsalute-7%2Fpatologie-68%2F0%2F0%2F4900%2Fit%2Fchiasso_line.xml&p=salmonella+typhi&type=jpeg&no=9&tt=172&ei=UTF-8http://rds.yahoo.com/S=96062857/K=salmonella+typhi/v=2/SID=w/l=II/R=4/SS=p/OID=de1a3a763177e736/SIG=1i4oks9bt/EXP=1122535007/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Btyphi%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-2481628037_q-X64O6BvAYaSHj.HChHAX7AABAA%40%40&h=193&w=197&imgcurl=www.dmacgroup.com%2FSalmonella%2520Typhi.jpg&imgurl=www.dmacgroup.com%2FSalmonella%2520Typhi.jpg&size=27.1kB&name=Salmonella%20Typhi.jpg&rcurl=http%3A%2F%2Fwww.dmacgroup.com%2Ftyphoid.htm&rurl=http%3A%2F%2Fwww.dmacgroup.com%2Ftyphoid.htm&p=salmonella+typhi&type=jpeg&no=4&tt=172&ei=UTF-8


46/87

Microbiology HTL

SalmonellaResistance:

killed at 60

degrees for 15 - 20

minutes

can survive whole

winter, 7 days in

well water

Typhoid & entericfever- S.typhosa, S.

para A, S. para B
http://rds.yahoo.com/S=96062857/K=salmonella+typhi/v=2/SID=w/l=II/R=11/SS=p/OID=20e9a0b452df5122/SIG=1l8htksf3/EXP=1122535007/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Btyphi%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-2481628037_q-X64O6BvAYaSHj.HChHAX7AABAA%40%40&h=302&w=302&imgcurl=www.spiceisle.com%2Fhomepages%2Fzross%2FMAC%2520Sal%2520typhi.jpg&imgurl=www.spiceisle.com%2Fhomepages%2Fzross%2FMAC%2520Sal%2520typhi.jpg&size=15.4kB&name=MAC%20Sal%20typhi.jpg&rcurl=http%3A%2F%2Fwww.spiceisle.com%2Fhomepages%2Fzross%2FEnteric%2520Demo.htm&rurl=http%3A%2F%2Fwww.spiceisle.com%2Fhomepages%2Fzross%2FEnteric%2520Demo.htm&p=salmonella+typhi&type=jpeg&no=11&tt=172&ei=UTF-8http://rds.yahoo.com/S=96062857/K=salmonella+typhi/v=2/SID=w/l=II/R=3/SS=p/OID=1f69b132783a9bec/SIG=1makhs926/EXP=1122535007/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Btyphi%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-2481628037_q-X64O6BvAYaSHj.HChHAX7AABAA%40%40&h=400&w=500&imgcurl=skywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2FSalmonella%2520typhi.jpg&imgurl=skywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2FSalmonella%2520typhi.jpg&size=21.4kB&name=Salmonella%20typhi.jpg&rcurl=http%3A%2F%2Fskywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2Fmicrow.htm&rurl=http%3A%2F%2Fskywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2Fmicrow.htm&p=salmonella+typhi&type=jpeg&no=3&tt=172&ei=UTF-8


47/87

Microbiology HTL

Salmonella

Paratyphoid fever -

S. paratyphosa

Gastroenteritis &food poisoning - S.

derby, S. enteritidis

Bacteremia,

septicemia- S.

Cholrasuis

Characteristics in food

poisoning: no taste or

smell, grow rapidly in

meat, toxins resistantover 100 degrees, not

destroyed with ordinary

cooking

Sources: water, milk &

dairy products, frozen

eggs, meat


48/87

Microbiology HTL

Salmonella Shigella

Motility non-motile motile

Hydrogen

sulfide

positive negative

Indol negative variable

Citrate positive negativeMannitol non fermenter fermenter


49/87

Microbiology HTL
http://rds.yahoo.com/S=96062857/K=salmonella+shigella+agar/v=2/SID=w/l=II/R=10/SS=p/OID=8c3633bbf60ddea2/SIG=1ilvnuq7g/EXP=1122535130/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Bshigella%2Bagar%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=446&w=437&imgcurl=www.euronet.nl%2Fusers%2Fwarnar%2Fbacterialplates.jpg&imgurl=www.euronet.nl%2Fusers%2Fwarnar%2Fbacterialplates.jpg&size=25.0kB&name=bacterialplates.jpg&rcurl=http%3A%2F%2Fhome.euronet.nl%2Fusers%2Fwarnar%2Fbiotechnologie2.htm&rurl=http%3A%2F%2Fhome.euronet.nl%2Fusers%2Fwarnar%2Fbiotechnologie2.htm&p=salmonella+shigella+agar&type=jpeg&no=10&tt=12&ei=UTF-8http://rds.yahoo.com/S=96062857/K=salmonella+shigella+agar/v=2/SID=w/l=II/R=2/SS=p/OID=e4ce39edb0155db0/SIG=1lvqav0hb/EXP=1122535130/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Bshigella%2Bagar%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=84&w=125&imgcurl=www.laboratoriosmicrokit.com%2Fimages%2F1de3%2F07-SS%2520Agar-E.coli%2520y%2520Salmonellap.jpg&imgurl=www.laboratoriosmicrokit.com%2Fimages%2F1de3%2F07-SS%2520Agar-E.coli%2520y%2520Salmonellap.jpg&size=12.1kB&name=07-SS%20Agar-E.coli%20y%20Salmonellap.jpg&rcurl=http%3A%2F%2Fwww.laboratoriosmicrokit.com%2Fdocs%2FTitle%2BGF-08b.htm&rurl=http%3A%2F%2Fwww.laboratoriosmicrokit.com%2Fdocs%2FTitle%2BGF-08b.htm&p=salmonella+shigella+agar&type=jpeg&no=2&tt=12&ei=UTF-8http://rds.yahoo.com/S=96062857/K=salmonella+shigella+agar/v=2/SID=w/l=II/R=6/SS=p/OID=9057b899882709a6/SIG=1nfgq5eoj/EXP=1122535130/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Bshigella%2Bagar%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=459&w=648&imgcurl=www.rci.rutgers.edu%2F%7Emicrolab%2Fapplied%2520micro%2Fapplied%2520images%2Fsalmshigella.png&imgurl=www.rci.rutgers.edu%2F%7Emicrolab%2Fapplied%2520micro%2Fapplied%2520images%2Fsalmshigella.png&size=1.1MB&name=salmshigella.png&rcurl=http%3A%2F%2Fwww.rci.rutgers.edu%2F%7Emicrolab%2Fapplied%2520micro%2Fschedulelinks%2Fsalmonellashigella.htm&rurl=http%3A%2F%2Fwww.rci.rutgers.edu%2F%7Emicrolab%2Fapplied%2520micro%2Fschedulelinks%2Fsalmonellashigella.htm&p=salmonella+shigella+agar&type=png&no=6&tt=12&ei=UTF-8


50/87

Microbiology HTL

Shigella

Shigella dysenteriae:

small, convex,

circular, colorless,glistening colonies

non-lactose

fermenter, body

temp. , aerobic

ferment glucose,

mannitol, citrate (-)
http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=12/SS=p/OID=685b6ac33decd958/SIG=1juo5gco4/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=400&w=500&imgcurl=skywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2FShigella%2520sonnei.jpg&imgurl=skywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2FShigella%2520sonnei.jpg&size=16.0kB&name=Shigella%20sonnei.jpg&rcurl=http%3A%2F%2Fskywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2Fmicrow.htm&rurl=http%3A%2F%2Fskywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2Fmicrow.htm&p=shigella&type=jpeg&no=12&tt=643&ei=UTF-8


51/87

Microbiology HTL

Mannitol fermentation

(-) Indole

(-) S. dysenteriae

(+) S. ambigua

(+) Lactose

(-) Indole

(-) S. flexner

(+) S. boydii

(+) Indole

(-) S. sonnei
http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=7/SS=p/OID=39457cd217194c2a/SIG=1j2r3u43h/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=320&w=400&imgcurl=medicalimages.allrefer.com%2Flarge%2Fdigestive-system.jpg&imgurl=medicalimages.allrefer.com%2Flarge%2Fdigestive-system.jpg&size=17.1kB&name=digestive-system.jpg&rcurl=http%3A%2F%2Fhealth.allrefer.com%2Fhealth%2Fshigella-enteritis-digestive-system.html&rurl=http%3A%2F%2Fhealth.allrefer.com%2Fhealth%2Fshigella-enteritis-digestive-system.html&p=shigella&type=jpeg&no=7&tt=643&ei=UTF-8http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=2/SS=p/OID=9c423650b0234ef0/SIG=1j80uum1k/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=120&w=160&imgcurl=toronto.cbc.ca%2Fgfx%2FToronto%2Fphotos%2Fshigella_052002.jpg&imgurl=toronto.cbc.ca%2Fgfx%2FToronto%2Fphotos%2Fshigella_052002.jpg&size=9.1kB&name=shigella_052002.jpg&rcurl=http%3A%2F%2Ftoronto.cbc.ca%2Fregional%2Fservlet%2FView%3Ffilename%3Dshigella_310502&rurl=http%3A%2F%2Ftoronto.cbc.ca%2Fregional%2Fservlet%2FView%3Ffilename%3Dshigella_310502&p=shigella&type=jpeg&no=2&tt=643&ei=UTF-8http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=3/SS=p/OID=a94a660e134e4926/SIG=1h76g1k5o/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=1258&w=1804&imgcurl=www-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro%2Fshigella.jpg&imgurl=www-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro%2Fshigella.jpg&size=961.7kB&name=shigella.jpg&rcurl=http%3A%2F%2Fwww-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro&rurl=http%3A%2F%2Fwww-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro&p=shigella&type=jpeg&no=3&tt=643&ei=UTF-8


52/87

Microbiology HTL

Diagnosis :

Stool exam, rectal swab --

culture in McConkey, SSAor EMB

Pathogenesis: bacillary

dysentery- abdominal

pain, cramps, diarrhea,

fever
http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=3/SS=p/OID=a94a660e134e4926/SIG=1h76g1k5o/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=1258&w=1804&imgcurl=www-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro%2Fshigella.jpg&imgurl=www-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro%2Fshigella.jpg&size=961.7kB&name=shigella.jpg&rcurl=http%3A%2F%2Fwww-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro&rurl=http%3A%2F%2Fwww-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro&p=shigella&type=jpeg&no=3&tt=643&ei=UTF-8http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=3/SS=p/OID=a94a660e134e4926/SIG=1h76g1k5o/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=1258&w=1804&imgcurl=www-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro%2Fshigella.jpg&imgurl=www-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro%2Fshigella.jpg&size=961.7kB&name=shigella.jpg&rcurl=http%3A%2F%2Fwww-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro&rurl=http%3A%2F%2Fwww-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro&p=shigella&type=jpeg&no=3&tt=643&ei=UTF-8http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=20/SS=p/OID=4befa7f204ac847c/SIG=1gi95af47/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=125&w=100&imgcurl=www.lcusd.com%2Flchs%2Fmewoldsen%2Fshigella.jpg&imgurl=www.lcusd.com%2Flchs%2Fmewoldsen%2Fshigella.jpg&size=3.0kB&name=shigella.jpg&rcurl=http%3A%2F%2Fwww.lcusd.com%2Flchs%2Fmewoldsen%2FFarag.html&rurl=http%3A%2F%2Fwww.lcusd.com%2Flchs%2Fmewoldsen%2FFarag.html&p=shigella&type=jpeg&no=20&tt=643&ei=UTF-8http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=4/SS=p/OID=3343a29f324f2818/SIG=1l1048cm5/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=772&w=580&imgcurl=www.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2Fgraphics%2F347shigella1.jpg&imgurl=www.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2Fgraphics%2F347shigella1.jpg&size=110.9kB&name=347shigella1.jpg&rcurl=http%3A%2F%2Fwww.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2F60_347.htm&rurl=http%3A%2F%2Fwww.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2F60_347.htm&p=shigella&type=jpeg&no=4&tt=643&ei=UTF-8


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Microbiology HTL

ArizonaArizona hinshawii

Gram (-) , motile,

similar to Salmonella

Slow lactosefermenter, sucrose &

Indole (-)

IMViC - -+-+Pathogenesis: causes

gastroenteritis or

sepsis

SPACE

FOR

RENT!!!


54/87

Microbiology HTL

Klebsiella

Klebsiella

pneumoniae (K.

oxytoca or

Friedlanders bacilli)

short, Gram (-) , non-motile, posess thick

capsule of

polysaccharide,facultative

anaerobes, killed at

55 degrees
http://rds.yahoo.com/S=96062857/K=klebsiella/v=2/SID=w/l=II/R=2/SS=p/OID=c1969b27ca0dc6a8/SIG=1gv3mjicp/EXP=1122535421/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dklebsiella%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=432&w=576&imgcurl=www.gcarlson.com%2Fimages%2FKlebsiella-2reduced.jpg&imgurl=www.gcarlson.com%2Fimages%2FKlebsiella-2reduced.jpg&size=77.9kB&name=Klebsiella-2reduced.jpg&rcurl=http%3A%2F%2Fwww.gcarlson.com%2Fmicrobial_krebsiella.htm&rurl=http%3A%2F%2Fwww.gcarlson.com%2Fmicrobial_krebsiella.htm&p=klebsiella&type=jpeg&no=2&tt=332&ei=UTF-8


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Microbiology HTL

Cultural char.:

large, mucoidcolonies, tenaceous

colonies, repeated

culture loses capsule

Biochemical:

ferment lactose,

sucrose, maltose,

mannitol & salicin

IMViC - --++
http://rds.yahoo.com/S=96062857/K=klebsiella/v=2/SID=w/l=II/R=3/SS=p/OID=c67c17784c488a5a/SIG=1kdvfngho/EXP=1122535421/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dklebsiella%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=400&w=500&imgcurl=skywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2FKlebsiella%2520pneumoniae.jpg&imgurl=skywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2FKlebsiella%2520pneumoniae.jpg&size=15.3kB&name=Klebsiella%20pneumoniae.jpg&rcurl=http%3A%2F%2Fskywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2Fmicrow.htm&rurl=http%3A%2F%2Fskywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2Fmicrow.htm&p=klebsiella&type=jpeg&no=3&tt=332&ei=UTF-8


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Microbiology HTL

Pathogenesis: causes

bacterial pneumonia,enteritis, Pharyngitis,

sinusitis, septicemia,

meningitis,

peritonitis, UTIAntigenic structure:

72 capsular types

Type 1&2 -respiratory

Type 8,9,10 - UTI
http://rds.yahoo.com/S=96062857/K=klebsiella/v=2/SID=w/l=II/R=15/SS=p/OID=7cd22e257dfb683e/SIG=1ikiqarbl/EXP=1122535421/*-http%3A//images.search.yahoo.com/search/i

Documents

Microbiology Module 7 - Bacilli