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Microbial Catalase for Personal Healthcare and Industrial Applications

Microbial Catalase for Personal Healthcare and Industrial ...€¦ · Catalase from Corynebacterium glutamicum: A Recombinant Microbial Enzyme for High-Level Applications Choose Roche

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Page 1: Microbial Catalase for Personal Healthcare and Industrial ...€¦ · Catalase from Corynebacterium glutamicum: A Recombinant Microbial Enzyme for High-Level Applications Choose Roche

Microbial Catalasefor Personal Healthcare and Industrial Applications

Page 2: Microbial Catalase for Personal Healthcare and Industrial ...€¦ · Catalase from Corynebacterium glutamicum: A Recombinant Microbial Enzyme for High-Level Applications Choose Roche

Catalase from Corynebacterium glutamicum: A Recombinant Microbial Enzyme for High-Level Applications

Choose Roche Catalase from Corynebacterium glutamicum for processes demanding high performance and safety. Specifically developed for use in contact lens cleaning systems, this qualified enzyme has seen more than 20 years of successful use.

GMP facility for the decomposition of biomass

Superior performance compared to bovine-derived enzyme: ��Save costs by reducing enzyme input (see Figures 1 and 2).

Well-established large-scale production process: ��Bulk quantities in giga unit dimensions are available.

No risk of Bovine Spongiform Encephalopathy (BSE): �� No animal-derived components are used in the production process.

Safe host organism for production:�� The production strain is a nonpathogenic, WHO Group I organism, widely used in the food industry.

Two formulations�� Available as a lyophilizate and as a glycerol/ ethanol solution.

Figure 2: Reduction potential. Tests show that only 150 IU of Roche Catalase from Corynebacterium remove 3% hydrogen peroxide in 10 minutes. By comparison, this is half the required amount of bovine-sourced catalase.

H2O

2 co

ncen

trat

ion,

%

Catalase, Corynebacterium

300 IU 200 IU 150 IU 100 IU

Time (minutes)

1

0 5 10 15

2

3

Figure 1: Removal efficiency of H2O2. Catalase from Corynebacterium glutamicum is very stable in the presence of hydrogen peroxide. Only small amounts of enzyme are required for eliminating H2O2. Using equal amounts of enzyme activity (300 IU), a 3% solution of H2O2 is removed significantly faster by Catalase from Corynebacterium than by Catalase from bovine liver.

H2O

2 co

ncen

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ion,

%

Catalase, 300n IU Corynebacterium Catalase, bovine liver

Time (minutes)

1

0 5 10 15

2

3

Microbial Catalase is for further processing only.

Page 3: Microbial Catalase for Personal Healthcare and Industrial ...€¦ · Catalase from Corynebacterium glutamicum: A Recombinant Microbial Enzyme for High-Level Applications Choose Roche

Specifications

Catalog Number 11 650 645 103 11 668 153 103

Nomenclature EC 1.11.1.6 EC 1.11.1.6

Appearance brown lyophilizate brownish-green, clear solution in glycerol/ethanol

Activity (25°C, H2O2) 11,000 – 17,000 U/mg lyophilizate  500,000 U/ml

Purity (HPLC, TSK 3000; A280)  90 area%  90 area%

Bioburden (acc. to DAB 10)Total Amount Escherichia coli, Salmonellae, Staphylococcus aureus, Pseudomonas aeruginosa

 50 CFU/g lyophilizate, below the limit of detection according to current QC procedures

 15 CFU/ml, below the limit of detection according to current QC procedures

pH Value (10 mg/ml H2O) 7.0 – 8.0 7.0 – 8.0

Water (K. Fischer)  5% no limit

Solubility (TK sucrose, Catalog No. 10 139 041) clear, soluble –

Sucrose (TC sucrose, Catalog No. 01 139 041)  60% –

Density (acc. to DAB 10) – no limit

Glycerol (enzymatic) – 310 – 430 mg/ml (25 – 35%; v/v)

Ethanol (enzymatic) – 71 – 87 mg/ml (9 – 11%; v/v)

Stability 18 months at 2°C – 8°C 18 months at 2°C – 8°C

The lyophilizate is available in sieved quality upon request.

Catalase from Corynebacterium glutamicum is more stable than catalase from bovine liver in all pH and temperature ranges, providing more flexibility and robustness in industrial applications (see Figures 3 and 4).

Figure 3: Temperature stability. Incubation: 15 minutes; pH 7.0; K-phosphate buffer, 50 mmol/l; 32 KU catalase/ml

Figure 4: pH stability. Incubation: 23°C, 15 hours; pH 7.0; K-phosphate buffer, 50 mmol/l; 16 KU catalase/ml

0 3 4 5 6 7 8 9 10 11 12

100

50

10

Rem

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pH value

Catalase, Corynebacterium

Catalase, bovine liver

Temperature, °C0 10 20 30 40 50 60 70 80 90

100

50

10

Rem

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ctiv

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Catalase, Corynebacterium

Catalase, bovine liver

Microbial Catalase is for further processing only.

Page 4: Microbial Catalase for Personal Healthcare and Industrial ...€¦ · Catalase from Corynebacterium glutamicum: A Recombinant Microbial Enzyme for High-Level Applications Choose Roche

Customers in Europe, Middle East, Africa, and Latin America:Roche Diagnostics Deutschland GmbHSandhofer Straße 11668305 Mannheim, GermanyPhone +49 621 759 8580Fax +49 621 759 8610

Customers in the United States:Roche Diagnostics CorporationRoche Applied Science9115 Hague RoadP.O. Box 50414Indianapolis, IN 46250-0414, USAPhone +1 800 428 5433, ext. 14649 (toll-free)Fax +1 317 521 4065

Customers in Canada:Roche Diagnostics201, Boulevard Armand-FrappierHV 4A2 Laval, Québec, CanadaPhone +1 450 686 7050Fax +1 450 686 7012

Customers in Japan:Roche Diagnostics K.K.Roche Custom BiotechApplied Science6-1, Shiba 2-chomeMinato-ku, Tokyo 105-0014, JapanPhone +81 3 5443 5285Fax +81 3 5443 7934

Customers in Asia Pacific:Roche Diagnostics Asia Pacific Pte. Ltd.Regional Sales and Market Development298 Tiong Bahru Road# 16-01/06 Central PlazaSingapore, 168730Phone +65 6371 6638Fax +65 6371 6601

Your Roche Custom Biotech Customer Service

custombiotech.roche.com

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