GRAM-POSITIVE BACTERIAGram-positive cocciStaphylococcusGram stain: Gram-positiveMicroscopic appearance: cocci in grape-like clustersOxygen relationship: facultatively anaerobic bacteriaMotility: nonmotileCatalase test: catalase-positiveOxidase test: negative*Spores: non-spore forming* Some species (non-human isolates) are positivestreptococcus imageStreptococcusGram stain: Gram-positiveMicroscopic appearance: cocci in chains (liquid media)or pairsOxygen relationship: facultatively anaerobic bacteriaMotility: nonmotileCatalase test: catalase-negativeOxidase test: negativeSpores: non-spore formingenterococcus imageEnterococcusGram stain: Gram-positiveMicroscopic appearance: cocci or ovoid cocci in pairs, clusters or short chains (liquid media)Oxygen relationship: facultatively anaerobic bacteriaMotility: nonmotile or motileCatalase test: catalase-negativeOxidase test: negativeSpores: non-spore formingListeria imageListeriaGram stain: Gram-positiveMicroscopic appearance: short rods (sometimes coccoid forms), single or in chainsOxygen relationship: facultatively anaerobic bacteriaMotility: motile (at 20-25C)*; peritrichous flagellaCatalase test: catalase-positiveOxidase test: negativeSpores: non-spore forming* optimal growth temperature at 30-37CCorynebacterium CorynebacteriumGram stain: Gram-positiveMicroscopic appearance: rods, often broader at one end("club" shape), grouped togetherin a characteristic way ("V", "palisades", "Chinese letters")Oxygen relationship: facultatively anaerobic bacteriaMotility: nonmotileCatalase test: catalase-positiveOxidase test: negativeSpores: non-spore forming**metachromatic granules are often present.Bacillus imageBacillus Gram stain: Gram-positiveMicroscopic appearance: rods in chainsOxygen relationship: aerobic or facultatively anaerobic bacteriaMotility: motile (peritrichous flagella) or nonmotile (e.g., B.anthracis)Catalase test: catalase-positiveOxidase test: negativeSpores: spore forming (endospores)ACID-FAST BACTERIAmycobacteriaMycobacteriumGram stain: - (acid - fast)Microscopic appearance: rods; sometimes cocobacilli or filamentsOxygen relationship: aerobesMotility: nonmotileCatalase test: positiveOxidase test: negativeSpores: non-spore forming