MethedologySCN Resistant Soy inoculated with Rhizobium , C.elegans and Paecilomyces lilacinus

SCN Resistant Soy inoculated with Rhizobium and C.elegans

SCN Resistant Soy inoculated with Resistant Rhizobium , C.elegans and copper sulfate

SCN Resistant Soy inoculated with Rhizobium , C.elegans and Chitin

SCN Resistant Soy inoculated with Rhizobium , C.elegans and Clove Oil

SCN Resistant Soy inoculated with Rhizobium , C.elegans and Pasteria Penetrans

ｷ Plant HeightThe soy will be grown in individual pots with a sand soil mixture and the varidables will be measured:ｷ Nodule numberｷ C.elegan countｷ Nitrogen Content ｷ Leaf Numberｷ Leaf Quality

X5

The mean will be calculated + standard deviation and the data will be analysed with and ANOVA and Sheffe post hoc test

SCN Resistant Soy, Chitin, Pasteuria Penetrans, Rhizobium, and C.Elegans

SCN Resistant Soy, Clove Oil, Chitin, Rhizobium, and C.Elegans

Rhizobium Leguminosarum

Culture Rhizobium in petri dishes with agar that has a concentration of (1,2,3,4mM CuSo 4

) and collect surviving rhizobium

Collect surviving Rhizobium and culture them at a higher concentration.

This graph shows the evolution of a resistant strain of Rhizobium. The x axis shows trial while the y axis shows resistance to Copper Sulfate (Cuso4). The resistance increased after each trial and got up to 18 millimoles.

This graph shows average nodule number in each pot. The x axis shows groups while the y axis shows nodule number. Group 2 had the most and group 3 had the least. A statistical analysis by an ANOVA was taken (p<.05), followed by a LSD post hoc test. (n=120)

This graph shows the average size of the nodules. The x axis shows group and the y axis shows nodule size in mm. Group 2 had the largest nodules and group 3 had the smallest nodules. A statistical analysis by an ANOVA was taken (p<.05), followed by

a LSD post hoc test. (n=120)

This graph shows average nitrogen plant and soil content. The x axis shows group while the y axis shows content. Groups 1 and 2 were the highest and had the same amounts and group 3 had the lowest amount. A statistical analysis by

an ANOVA was taken (p<.05), followed by a LSD post hoc test. (n=120)

This graph shows the average amount of C.Elegans in each pot. The x axis shows groups while the y axis shows C.Elegans. Group 3 had the most and Group 5 had the least , while group 6 was in between.

A statistical analysis by an ANOVA was taken (p<.05), followed by a LSD post hoc test. (n=120)

This graph shows the average height of each plant. The x axis shows the groups while the y axis shows the height in cm. The plants in group 3 were the tallest and in group 1 were the shortest , while group 6 was in between. A statistical analysis by an ANOVA

was taken (p<.05), followed by a LSD post hoc test. (n=120)

This graph shows the average percentage of healthy leaves per plant. The x axis show the groups while the y axis shows the percentage. Group 3 had the most healthy leaves and group 5 had the most unhealthy leaves , while group 6 was in between. A statistical analysis

by an ANOVA was taken (p<.05), followed by a LSD post hoc test. (n=120)

This graph shows the average number of leaves per plant in each group. The x axis shows the groups while the y axis shows the amount of leaves. Group 3 had the most leaves and group 1 had the least, while group 6 was in between. A statistical analysis by an ANOVA was taken (p<.05), followed by a LSD post hoc test. n=120

This graph shows average nitrogen plant and soil content. The x axis shows group while the y axis shows content. Groups 1 and 2 were the highest and had the same amounts and group 3 had the lowest amount. A statistical

analysis by an ANOVA was taken (p<.05), followed by a LSD post hoc test. (n=120)

Paecilomyces lilacinus

Control Clove Oil Chitin Pasteuria Penetrans

Resistant Rhizobium and CUSO4

Chitin and Pasteuria Penetrans

Chitin and Clove Oil

Paecilomyces lilacinus

Control Clove Oil Chitin Pasteuria Penetrans

Resistant Rhizobium and CUSO4

Chitin and Pasteuria Penetrans

Chitin and Clove Oil

Paecilomyces lilacinus

Control Clove Oil Chitin Pasteuria Penetrans

Resistant Rhizobium and CUSO4

Chitin and Pasteuria Penetrans

Chitin and Clove Oil

Paecilomyces lilacinus

Control Clove Oil Chitin Pasteuria Penetrans

Resistant Rhizobium and CUSO4

Chitin and Pasteuria Penetrans

Chitin and Clove Oil

Paecilomyces lilacinus

Control Clove Oil Chitin Pasteuria Penetrans

Resistant Rhizobium and CUSO4

Chitin and Pasteuria Penetrans

Chitin and Clove Oil

Paecilomyces lilacinus

Control Clove Oil Chitin Pasteuria Penetrans

Resistant Rhizobium and CUSO4

Chitin and Pasteuria Penetrans

Chitin and Clove Oil

Paecilomyces lilacinus

Control Clove Oil Chitin Pasteuria Penetrans

Resistant Rhizobium and CUSO4

Chitin and Pasteuria Penetrans

Chitin and Clove Oil

Paecilomyces lilacinus

Control Clove Oil Chitin Pasteuria Penetrans

Resistant Rhizobium and CUSO4

Chitin and Pasteuria Penetrans

Chitin and Clove Oil