Metabolism

( all the chemical reactions occur in a cell )

Catabolism ( breaking down of molecules )

- usually releases energy

Aanabolism ( synthesis of molecules )

- usually requires energy

Amino acids may not be linked together but are close distance to each other

Other amino acids for maintaining

the correct globular structure

E + S ES complex EP complex E + P

Compare and contrast the “lock and key hypothesis” and

“induce fit hypothesis”

Similarities :

1. Similar reaction sequence : E+S ->ES complex ->EP complex ->E+P

2. Catalytic reaction occurs in active site of enzyme

Differences LKH IFH

1. Initial shape of active site

exactly fit with substrate

may not be exactly right in shape to fit the

substrate2. Flexibility of

enzyme molecule less more

3. Change in shape of active site no yes, induced by the binding of

substrate with active site

Activator (inorganic )

A B C

Rate of enzymatic

reaction

Factor :

pH

Temperature

enzyme Conc

Substrate Conc

Coenzyme Conc

.

.Which is the limiting factor which

controls the rate of reaction ?

For fixed amount of substrate

Non competitive inhibitor

Competitive inhibitor