Mehsana District Cooperative Milk Producer’s Union Ltd. … · 2015. 9. 12. · Mehsana District Cooperative Milk Producer’s Union Ltd. MDCMPUL/CF/2015-16/01 Page 2 of 36

MDCMPUL/CF/2015-16/01

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For supply of good quality raw material in competitive price for Mehsana District Cooperative Milk Producer’s Union Ltd. Tender Document

2015

12/09/2015

Cattle Feed Raw Materials Purchase Tender Document For supply of good quality raw material in competitive price for Mehsana District Cooperative Milk Producer’s Union Ltd.

2015

12/09/2015


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Contents 1. General Information ............................................................................................................................. 3

1.1 Notice Inviting Tender ......................................................................................................................... 3

1.2 Downloading of Tender Document ..................................................................................................... 4

1.3 Bid Security ......................................................................................................................................... 5

1.4 Rights Reserved By Dudhsagar Dairy, Mehsana ................................................................................. 5

2. Annexure-I: General Terms and Conditions .......................................................................................... 5

3. Annexure-II: Technical Specifications of Cattle Feed Raw Materials .................................................... 9


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1. General Information

1.1 Notice Inviting Tender

Name of Company Mehsana District Co-operative Milk Producers’ Union Ltd, Dudhsagar Dairy Highway,Mehsana-384002

Tender Notice No. MDCMPUL/CF/2015-16/01

Name of Work Purchase cattle feed raw material for our cattle feed plant at Jagudan and Ubkhal

Bid Documents Download Start 12th Sep 2015

Bid Documents Download End 18th Sep 2015 15:00Hrs

Last Date & Time for Receipt of Bids

18th Sep 2015 15:00Hrs Note: Physical submission of Rate with quantity and Tender fee in sealed cover on or before 18th Sep 2015 15:00Hrs in the office of Cattle Feed Raw Material Purchase Department, Mehsana District Co-operative Milk Producers' Union Ltd., Dudhsagar Dairy, Mehsana during office hours.

Online Tender Document Download Website

http://www.dudhsagardairy.coop/

Terms and Conditions Rate will be non negotiable and FOR to our cattle feed plant and printed on the letter head of the bidder

Minimum Bid Quantity Offered should be 50% of the tender quantity

If L1 is unable to match the full quantity required then L2 will be given the chance to match the L1 price for balance quantity and so on.

Single bid for one firm with all his sister/associate concern is permitted.

Bidder have to abide by all terms and conditions mentioned at Annexure-I.

Bidder should have to abide the specification of items mentioned at Annexure-II.


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Decision taken by the I/C Managing Director will be final

VAT/CST and PAN No are to be Provided with the tender

Tender Fee Rs.5000.00 non refundable

Bid Document Fee Payable To : Mehsana district Co-operative Milk producer’s Union Ltd , Mehsana

Bid Security/EMD (INR) :

Materials EMD (Rs) Poha Tukadi

100000 Rapseed Ext.

75000 R. P. Fine Gr.1 300000 Guar Chuni 40%

200000 D.O.R.Bran 225000 Rice Flakes Bran 75000 Urea For Industrial Use 50000 Grinding Salt

10000 Calcite Powder 10000

Bid Security/EMD in favor of : Mehsana District Co-operative Milk Producers' Union Ltd., Mehsana

Officer Inviting Bids I/C Managing Director

Bid Opening Authority Purchase Committee

Bid Opening Date 18th Sep 2015 16:00Hrs

Address : Cattle Feed Raw Material Purchase Department Mehsana District Co-operative Milk Producers’ Union Ltd, Dudhsagar Dairy Highway, Mehsana-384002

Contact Details : 02762-253201

1.2 Downloading of Tender Document

The Bid document can be downloaded from website http://www.dudhsagardairy.coop/. Tender fee will be (non-refundable) is Rs. 5000/- by Demand Draft from a Nationalized / Scheduled Bank in favor of Mehsana District Co-operative Milk Producers’ Union Limited, payable at Mehsana. It has to be submitted by Speed Post/Courier/in Person between 14 Sep 2015 to 18 Sep 2015 (15:00 Hrs) during office hours.


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1.3 Bid Security

All Bids must be accompanied by Earnest Money Deposit (EMD) in the form specified in the Bidding document. The Bids not accompanied with EMD shall be summarily rejected. The Bid security shall be denominated in Indian Rupees of value as specified and shall be in the form of Demand Draft from Nationalized or Scheduled banks in favor of Mehsana District Co-operative Milk Producers’ Union Limited, payable at Mehsana. The Bid security may be forfeited if:

A Bidder or Supplier withdraw its bid during the period of bid validity specified by the Purchase on the tender document or

In case of successful Bidder / Supplier, if the Bidder / Supplier fails to sign the contract or execute the contract within specified period of contract.

1.4 Rights Reserved By Dudhsagar Dairy, Mehsana

Dudhsagar Dairy, Mehsana, at its sole discretion and without assigning any reason thereof, reserves the right to accept and / or reject the whole or part of any all the Bids received.

2. Annexure-I: General Terms and Conditions 1. Acceptance :

a. This order will be issued in duplicate, based on the confirmation

received from you. You are requested to return duplicate copy of

the order duly signed and stamped within 4 days, in token of

acceptance of the same. Acceptance letter via mail same day and

hard copy within 4 days of PO issued is to done.

b. E-mail copy of the purchase order will be sent to the party and if

there is no communication is made for denial or approval within 3

working days of purchase order issue, it will be considered as

confirmation.

2. Quality :

i. The goods should meet the minimum quality parameters set by

the union for each type of materials. The goods should be free

from dirt, impurities of any kind, insects and fungus infestations

and metallic pieces.

ii. The goods should be free from any undesirable and

objectionable order of any kind.


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iii. The product samples will be tested in our laboratory and the test

results of our laboratory will be treated as final for purpose of

payment

iv. The union reserves the right to go or not for external testing of

sample and to accept or reject the results from outside

laboratories.

v. The union reserves the right to accept or reject any consignment

without assigning any reason and decision of the union in this

regard will be fine.

vi. The goods supplied should be suitable for manufacturing cattle

feed. The supplier shall be responsible for consequential losses /

liabilities arising due to supply of sub standard or poisonous

goods.

3. Quantity :

Excess Delivery: You should supply the goods as per the quantities mentioned in the order.

However union will accept quantity up-to 10% variation of order quantity or 25 MT whichever is lower

at same rate as mentioned in the Purchase order.

Short Delivery:

i. It is expected that all the suppliers meet their commitment of full supply

within the delivery period as mentioned in the PO.

ii. If the total quantity supplied is less than 90% of the PO quantity, the

differences in the cost of short supply quantity will be recovered from the

supplier as per following formula

Difference of cost to be recovered from supplier = (Q1 – Q2)* (R1 – R2)

Q1 = PO quantity in MTs

Q2 = Quantity Actually supplied in MTs

R1 = The rate prevailing on the last delivery date as per PO or next contracted rate

after the last delivery date in Rs./MT.

R2 = The PO rate in Rupees/MT

However, if the rate prevailing on the last delivery date or next available contracted rate is

lower than the PO rate, no recovery will be made from the supplier. The suppliers, who are


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found to be frequently defaulting on full delivery, may be blacklisted for any future business

with them.

4. Weighment :

The suppliers should weigh consignment before dispatching to us and weighment slip should always accompany the consignment. However, the weight recorded at our cattle feed plants will be treated as final for payment. In case of doubts, truck drivers or supplier’s representatives may weigh the consignment on private weigh bridge outside the factory premises and provide a weighment slip for verification at our end. The weight will be calculated on a net weight basis and a deduction of 200 grams per PLASTIC bag will be deducted from the weight of material. Similarly 1.5 kg will be deducted from GUNNY BAG.

5. Documents :

The reference of this purchase order n date should always be mentioned in all the delivery documents such as weighment slips, delivery challans, invoices etc. The goods received without this information will be treated as supplied without a purchase order or may be rejected.

6. Delivery :

The entire quantity of goods covered in a PO should be supplied within the delivery period as mentioned in the PO. For goods delivered beyond the delivery period / date, a delay penalty will be recovered after due date @ Rs 50/- per MT for 7days and @Rs 100/- per MT beyond. However, even after this extended period of delivery, if the quantity delivered in short of 90% of PO quantity, it will be dealt as per clause No. 3.2(ii) above. Under exceptional circumstances affecting timely delivery of goods, delivery period may be suitably extended by the appropriate authority. However, in such cases delay penalty or the cost of short delivery will be calculated considering this extended delivery period.

7. Cancellations :

The union reserves the right to cancel whole or part of purchase order by giving 3 days of written or telephonic intimation, within the validity period of the purchase order. However, beyond the validity of the Purchase Order (Including Pos with extended validity period) may be cancelled without giving any notice and without assigning any reasons.

8. Rejected Goods :


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i. The suppliers will be intimated, if any of the consignment is

rejected or any of the grounds, it will be responsibility of the

supplier to lift the rejected the goods within 30 days from the time

the supplier is given Telephonic / Fax intimation.

ii. If the rejected goods are not lifted within 30 days from the time

intimation is given to the supplier, a storage charge of Rs. 20

Mt/ day will be levied and the same will be recovered from

any of the subsequent bills of the supplier.

iii. If the rejected goods are not lifted within the stipulated time, the

union shall dispose the goods at the risk and responsibility of the

supplier.

9. Packing :

The goods should be supplied in sound gunny bags (new or old) or HDPE bags appropriate for the raw material supplied. The goods packed in torn or unsound condition of gunny bags may be rejected. In-case the bags in which the raw-material is supplied are torn there will be a deduction of Rs. 10/- per bag from the consignment supplies

10. Penalty:

i. Short supply/ NO supply/ Late delivery penalty will be charged

@ Rs. 200/- per Mt. Starting from the first day after the need by

date is over.

ii. In case of rejection of consignment if the party does not lift the

material within 30 days of the rejection. A charge of Rs 50/- per

MT per day will be charged this amount will be Rs.100/- per MT

per day after 30 days from rejection.

11. Payment :

The payment will be made from the 20 days from the date of the receipt, of goods or invoice whichever is later, by an A/C. Payee cheque drawn or any nationalised Bank at Mehsana. The payment can also be made by DD or cheque payable at the place of supplier’s choice after deducting the Demand Draft charges etc. The cheques or DDs will be sent to the supplier either by post or courier services.

12. Rebate /Premium /Specification : Annexure attached

13. Jurisdiction :

The courts at Mehsana shall be the jurisdiction for settlement of any

dispute relating to this order irrespective of what is slated in your

delivery documents, invoices etc.


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3. Annexure-II: Technical Specifications of Cattle

Feed Raw Materials

Acceptance Criteria including Specifications, Sampling Plan & Test

Methods to be followed to analyze Cattle Feed Raw Materials/Packing

Materials/Additives etc.

When the materials (consignment) will be reached at our site, the physical conditions and in case of

packaging materials, cleanliness will be thoroughly checked by the Store Personnel along Personnel of

the respective department; it is chiefly Quality Assurance.

Later, the physical quality parameters will be primarily checked by the quality assurance personnel

(Sampler & if, required; Head of the Department) before accepting the material & then, only; it will be

allowed for unloading at our inventory. If; the parameters will not meet the requirements (Physically),

the materials will be strictly rejected &/or may be accepted as a whole or partially in a special

circumstance considering the significance and variability of quality parameters & with only, kind

approval of Shri Managing Director Sir.

Also, the material already have been unloaded at our inventory, if; found inferior in quality when

tested chemically, the material (consignment) will be rejected & the supplier will have to lift-up the

rejected material (consignment) at earliest i.e within 15 days period of time.

SAMPLING PLAN

PACKING MATERIALS, RAW MATERIALS AND ADDITIVES:

Sr.

No.

Sample Details Frequency Size of Sample

Selection

Criteria

Sample

Quantity

1 Poha Tukadi Each Consignment 60 % Bags 1000 g

2 Rice Polish Fine Each Consignment 100% Bags 1000 g

3 Rapeseed Extraction Each Consignment 40-50% Bags 1000 g

4 DORB, Rice Flakes Each Consignment 40-50% Bags 1000 g

5 Guar Meal, Guar chuni Each Consignment 50% Bags 1000 g

6 Salt/Industrial grade

Urea

Each Consignment 25 - 30% 1000 g

7 Calcite Powder Each Consignment 50% Bags 1000 g


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SPECIFICATIONS OF CATTLE FEED RAW MATERIALS

Sr.

No

Specification

for

Tests Requirements

Specification Max./Min. Rebate

Limits UOM

1 D.O.R.B

Protein Min. 15.00 Down to 11.00 %

Moisture Max. 10.00 Up to 14.00 %

Silica

Max. 5.00 Up to 8.00 %

03.00 to 05.00 Premium will be @

1.00 %

Fiber Max. 11.00 Up to 15.00 %

2 R. P. Fine


Fat

Min. 16.00 Down to 12.00 %

Up to 20.00 Premium will be @

3.12 %

Silica

Max. 5.00 Up to 10.00 %

03.00 to 05.00 Premium will be @

1.00 %

3 Rapeseed

Extraction


Protein Min. 38.00 Down to 34.00 %


Silica Max. 2.50 Up to 5.00 %


4 Guar Chuni

(40%)


O + A Min. 40.00 Down to 36.00 %



5 Rice Flakes

Bran


O + A Min. 10.00 Down to 08.00 %



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Sr.

No

Specification

for

Tests Requirements

Specification Max./Min. Rebate

Limits UOM

6 Poha Tukadi

Dust Max. 1.00 Up to 3.00 %

Infestation Max. 5.00 Up to 30.00 %


Retention Min. 60.00 Down to 55.00

SPECIFICATION OF ADDITIVES

Sr.

No

Specification For Tests Requirement UOM

Specification Max./Min. Limits with

Discount

1 Urea

Nitrogen Min. 46.40 - %

Protein Min. 290.00 - %

2

Grinding/Whole

Salt

Purity Min. 98.00 Down to 96.00 %


3 Calcite Powder


Calcium Min. 37.00 Down to 36.00 Up to

40.00 %

Total Ash 54.00- 60.00 Down to 52.00 & Up to

62.00 %


TEST METHODS

Raw material, additives & packing material will be analyzed for its conformity to the laid down

requirement. Following references is used to prepare the test method. For specific instrument like

Dickey John/Foss NIR procedure provided by the manufacturer is used as reference.

References of testing methods:


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SR. NO. TEST REFERENCE

1 Sample Size IS:2052-1979, APPENDIX C

2 Moisture

Protein

Fat

Crude Fiber

Silica

Calcium

Phosphorus

Urea

Vitamin-A

Vitamin-D

Density

IS 7874 (Part-I) : 1975

IS 7874 (Part-I) : 1975

IS 7874 (Part-I) : 1975

IS 7874 (Part-I) : 1975

IS 7874 (Part-I) : 1975

NDDB Manual

NDDB Manual

NDDB Manual

NDDB Manual

NDDB Manual

NDDB Manual

RAW MATERIALS


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Sr.

No.

Product Name Reference Test Method

Moistu

re

Crude

Protein

Cru

de

Fat

Cru

de

Fibe

r

Tot

al

Ash

Silic

a Ca P Other

1

Grains

Juwar, Maize,

Damage

wheat, Bajri,

Barely etc

12

23.

Infestati

on

24. Dust

2 Rice Polish fine 12,11 13 14 16 17

3 Deoiled Rice

Bran 12,11 13,11

14,1

1 11 16

17,

11

4

Guar Bhardo/

Chuni/ Babul

Chuni /

Tamarind seed

powder

12,11 13 14 15,1

1 16

17,

11

5

Oil seed

extractions

and cakes

12,11 13,11 14,1

1

15,1

1 16 17

6

Purn milk

magic /SAGAR

BOOSTER

25 16 17 18 1

9

Material

will be

accepte

d on the

basis of

COA

results

7 Calcite powder

12 16 17 18

26 Mesh

Size

8 Grinding Salt

12

27

Purity

9 Urea/M M

SRN/Optigen

13

Material

will be

accepte

d on the

basis of

COA

results

10 VitaminAD3/Vi

tamin AD3 – Material

will be


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Double

strength

accepte

d on the

basis of

COA

results

11 Groundnut

Shell Powder

12 16 17

26

Retentio

n time

12 Dhania Husk 12 16 17

13 Molasses/Myc

o curb AW DS 12

20

Density

14 Wheat Bran 12 16 17

15 Bioplex High

Seven Organic

Mineral

Mixture

Material

will be

accepte

d on the

basis of

COA

results

16 Bypass Fat

Feed

12

By

differen

ce

IC

P-

OE

S

GC-FID

(By

NDDB)

11.0 ANALYSIS BY NIR Analyser (Dickey John/Foss)

NIR Analyser is a various purpose instrument. It gives the instant analysis of Moisture, Fat, Protein,

Fiber, Silica present in the sample.

11.1 Apparatus:

(a) NIR Analyser

11.2 Procedure:

Turn the power ON/OFF switch located on the rear of the unit, at the ON position.

Turning power on the hello message appears.

The TEMP message on the display remains lit until the machine temperature stabilizes.

The ‘HELLO’ message on the top display remains on until a product is selected.


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As numbers are entered via the keyboard, the results appear on the top display.

The Decimal/Arrow enters a decimal point and +/- key changes the sign of the number from positive

to negative.

Enter the product number, decimal point constituent numbers, and then depress the PROD key.

The selected product and constituent numbers appear on the center display. The center indicates the

selected product number, a decimal point and (-), indicating multiple constants have been selected.

The PRODUCT and constituent messages are black lit.

Clean the window of the sample cup. Brush the sample cup with a soft camel’s hairbrush.

Filled the cup with the prepared sample, place the sample cup cover on loading tray.

Place the cup with the glass window down twist the cup clockwise until it locks into place.

Do not get fingerprints on glass window.

Enter a sample ID number, of up to five digits, and the results shall be displayed. Use the Step Switch

to increment the Instalab display from one reading to the next.

Take out the print with the analysis in the Modes “o” or “1”. The moisture content shall be seen on

the screen of the NIR to which the print may be taken by the printer.

Note: Procedure to work with Foss NIR Analyser is more or less constant as of Dickey John.

12.0 DETERMINATION OF MOISTURE

12.1 Moisture Determination by Gravimetric Method:

12.1.1 Apparatus:

(a) Aluminum Dish: Dish with minimum diameter 50 mm and approx. depth 40 mm

(b) Hot air oven: Controlled at 135 ± 2 ºC

12.1.2 Procedure:

Accurately weigh the clean, dry empty aluminum dish with lid.

Accurately weigh about 5 gm of the prepared sample in the aluminum dish with cover and distribute

the content evenly by shaking the dish.

Place the dish in to a well-ventilated oven maintained at 135 ±2 0C for two hrs.

Place cover on dish and cool the dish in desiccators for about 30 min. and weigh the dish.

Repeat heating & cooling till successive weights of dish is less than one milligram.

12.1.3 Calculation:

Moisture, percent by mass = 100 (M1 – M2)

M1 – M

Where:


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M1 = mass in gm of the dish with material before drying

M2 = mass in gm of the dish with material after drying

M = mass in gm of the empty dish

12.2 Determination of Moisture by NIR Analyser:

Please refer operating method in 11.0

Determination of Moisture by AXIS LCGC ELECTRONIC MOISTURE ANALYSER:


13.0 DETERMINATION OF CRUDE PROTEIN

13.1 Determination of Crude Protein by Kjeldhal Method:

13.1.1 Principle:

The percentage of crude protein is ascertained by multiplying the percentage of total nitrogen by a

factor. For animal feeds and feeding stuffs the factor to be used will be 6.25, except in case of wheat

and its products for which it will be 5.70.

13.1.2 Glass wares:

Kjeldhal Flask: Made of borosilicate glass with 800ml capacity.

Glass Distillation assembly

Conical Flask: 250ml

Pipette: 25.00ml

Burette: 50ml with 0.1ml graduation

13.1.3 Reagents:

(a) Potassium Sulphate or Anhydrous Sodium Sulphate (K2SO4 or Na2SO4)

(b) Copper Sulphate (CuSO4, 5H2O)

Concentrated Sulphuric Acid: Sp. Gr. 1.84

Sodium Hydroxide Solution (45%): Dissolve about 450 g of Sodium hydroxide pallet and make it 1000

ml with distilled water.

Standard Sulphuric Acid: 0.5 N (14.2ml C. H2SO4 in 1000ml water).

(f) Standard Sodium Hydroxide Solution: 0.25 N (10g NaOH in 1000ml water).

(g) Methyl Red Mix Indicator: Take 100 mg Methyl Red & 30 mg of Methyl Blue and dissolve in 100ml

Rectified Spirit.

(h) Phenolphthalein Indicator: Take 1 gm of Phenolphthalein Indicator and dissolve in 100 ml Rectified

Spirit.

(i) Perchloric acid

13.1.4 Procedure:


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A. Digestion:

Transfer carefully 1 gm of the prepared sample to the dry Kjeldahl flask.

Add about 5 gm catalyst mixture (CuSO4 & K2SO4, ratio of 1:3) and 25 ml (or more if necessary) of

conc. H2SO4.

Add 1 ml Perchloric acid to reduce the digestion time.

Place the flask in an inclined position and heat below the boiling point of the acid until frothing

ceases. Continue heating until the acid boils vigorously and digest for a time after the mixture is clear.

After about 1.5 hour of digestion, cool the contents of the flask.

B. Distillation and Titration:

Then add about 200ml distilled water and add a few pieces of glass bid to prevent bumping.

Add carefully the sodium hydroxide solution in quantity, which is sufficient to make the solution

alkaline by the side of the flask so that it does not mix at once with the acid solution but forms a layer

below the acid layer.

Assemble the apparatus taking care that the tip of the dip tube extends below the surface of the

standard H2SO4 solution (0.5N) in the receiver.

Mix the contents of the flask by shaking and distil until all ammonia has passed over into the standard

H2SO4 solution.

Titrate with standard NaOH (0.25N) solution.

13.1.5 Calculation:

Total Nitrogen (percent by mass) = 140 x V x N

m

Where:

V = volume in ml of standard Sodium hydroxide used in titration

N = Normality of standard Sodium hydroxide

m = mass in g of the dried sample taken for the test.

Crude Protein (percent by mass) = 6.25 x Total Nitrogen

Crude Protein (%) = 0.21875 X 10 X r

Where:

r = (50 – R) and R = burette reading

13.2 Determination of Crude Protein by NIR Analyser:


13.3 Determination of Crude Protein by Semi-Automatic Equipment:



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14.0 DETERMINATION OF CRUDE FAT

14.1 Determination of Crude Fat by Extraction Method:

14.1.1 Principle:

The fat of cattle feed can be determined by Soxhlet extraction method.

14.1.2 Apparatus:

1. Soxhlet extraction Assembly: This consists of 3 parts (a) condenser at the top, (b) the soxhlet or

extractor in the middle, and (c) the receiver flask at the bottom. The 3 parts are assembled by means

of their ground glass interchangeable joints.

2. Extraction thimble

3. Heating coils with temperature controlling device.

14.1.3 Reagents:

1. Petroleum ether (Boiling Point 60ºC to 80ºC) or hexane food grade.

14.1.4 Procedure:

Weigh about 2 gms of prepared sample and transfer it in a thimble.

Plug the mouth of thimble with fat free absorbent cotton.

Take the clean, dry receiver flask from the soxhlet assembly and weigh it accurately.

Introduce the thimble with sample into the soxhlet. The height of the thimble should be of such size

that highest point it should remain below the bend of siphon.

Assemble the apparatus and fill soxhlet with petroleum ether or hexane food grade by pouring it

through the condenser at the top by means of a glass funnel. The amount of solvent taken is about 1

½ times the capacity of soxhlet.

Place the apparatus on the hot plate at 60 deg centigrade, fix by clamps to a retort stand and start

cold water circulation in the condenser. Extract for Four hours at a condensation rate of 5-6 drops per

second. After extraction is over, remove the thimble with the materiel from soxhlet.

Assemble the apparatus again and heat it on the water bath to recover all the ether or hexane from

the receiver flask. The flask now contains only the crude fat.

Wipe the outside surface of the receiver flask with a clean dry cloth and dry it in a hot air oven at

100ºC for one hour, cool in a desiccator & weigh. Continue at 30-min intervals this alternate drying

and weighing until the difference between two successive weighing is less than one mg. Note the

lowest mass. Preserve the extraction thimble with the material.

14.1.5 Calculation:

Crude fat percent = (M1 – M2) 100


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m

Where:

M1 = mass in g of the extraction flask with dried extract.

M2 = mass in g of the extraction flask


14.2 Determination of Crude Fat by Semi-Automatic equipment:

14.2.1 Principle:

The fat can be determined by Socs plus systems which is improved Soxhlet extraction method.

14.2.2 Apparatus:

1. Basic unit: The basic unit houses an extruded aluminum block and is heated to the required

temperature using plate heaters. The temperature of the block can be set and is also controlled

accurately using the micro processor based temperature controller. The set temperature and the

actual temperature are displayed digitally in the temperature controller. The block can be moved up

and down to fix the extraction beaker to the system.

2. Extractor: The extractor is made of high quality Borosil glass and is fixed with proper clamps to the

basic system. The solvent that evaporates from the beaker is condensed and collected in the

extractor. The condensed solvent can again be recycled by opening the Teflon stopper into the

beaker. Solvent can be added to the beaker through a funnel fitted to the extraction from the top of

the system. Water is circulated into the condenser which is built inside the extractor to condense the

evaporated solvents.

3. Beaker: The beaker is made of borosil glass, specially designed to suit the socs plus system. During

extraction, beaker houses the thimble along with the sample. The beakers are handled safely using

stainless steel rack.

4. Thimble Holder: Thimble holder is made of castlon moulded to the shape. This material is highly

inert to most of the solvents. The cellulose thimble is fixed tightly to the thimble holder. The thimble

holder is placed on the step provided inside the beaker. The thimble is removed from the thimble

holder only if it gets damaged. These thimble holders are kept safely into a stainless steel rack.

14.2.3 Reagents:

1. Petroleum ether (Boiling Point 60ºC to 80ºC) or hexane food grade.

14.2.4 Procedure:

Switch on the ‘Power Switch’ and ensure the set and actual temperature on the display.

Wash the beaker thoroughly and weigh to know the empty weight of the beaker.

Weigh the sample and transfer into cellulose thimble.

Open the water tap and ensure flow of water through the water condenser.


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Keep the thimble holder along with the sample into the beaker.

Take required quantity of solvent into the beaker.

Take the beakers to the main unit using the beaker trays, pull the slider of the aluminum block down

and load the beakers into the system. Ensure perfect sealing of the beakers against the Teflon ring.

Set required temperature in the controller.

Once the required temperature is reached recovery of solvents is noticed.

Open the stopper, so that the recovered solvent is allowed to flow to the beaker to maintain the same

level, on doing this. The boiling stage and the extraction stage get completed.

The time and the samples contact with the boiling solvent varies depending on the nature of sample

and percentage of fat.

After the completion of the boiling period close the stopper, in order to collect the solvents in the

solvent compartment.

During recovery, when the level of solvent touches below the bottom of the thimble, open the

stopper and ensure the rate of condensation of solvent and the delivery of the solvent are at

equilibrium. This stage of operation is called ‘rinsing’.

The period of rinsing varies with nature of sample and percentage of fat.

At the end of the rinsing stage close the stopper tightly. Recover all the solvent in the extractor.

Remove the beaker along with the fat and keep inside the oven for 30 min. and take the weight of the

beaker.

14.2.5 Calculation:

Crude fat percent = (M1 – M2) 100

m

Where:

M1 = mass in g of the extraction beaker with dried extract.

M2 = mass in g of the extraction beaker.


14.3 Determination of Fat by NIR Analyser:


15.0 DETERMINATION OF CRUDE FIBER

15.1 Determination of Crude Fiber by Chemical Method:

15.1.1 Glassware & Instrument:

Conical Flask; 1 liter capacity

Condenser


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Gooch Crucible

Muffle furnace

15.1.2 Reagents:

1. Sulfuric acid solution: 0.255N (1.25% m/v or 7.2ml C. H2SO4 in 1000ml water)

2. Sodium Hydroxide solution: 0.313N (1.25% m/v), accurately prepared

15.1.3 Procedure:

Weigh accurately about 2 g of the sample material and extract the fat for about 8 hours with

petroleum ether or hexane (food grade) using Soxhlet or other suitable extractor.

Transfer the fat free dry residue to a one-liter conical flask.

Take 200ml of 1.25 % Sulphuric acid and bring to the boil. Transfer the whole of the boiling acid to the

flask containing the fat free material.

Immediately connect the flask with a reflux water condenser & heat, so that the contents of the flask

begin to boil within one minute. Rotate the flask frequently, taking care to keep the material from

remaining on the sides of the flask out of contact with acid. Continue boiling for exactly 30 minutes.

Remove the flask & filter through fine linen (about 18 threads to the centimeter: Muslin clothe) held

in a funnel and wash with boiling water until the washings are no longer acid to litmus.

Bring to the boil same quantity of Sodium Hydroxide Solution under a reflux condenser.

Wash the residue on the linen into the flask with 200 ml. of boiling Sodium Hydroxide solution (1.25%)

& immediately connect the flask with the reflux condenser & boil for exactly 30 minutes.

Remove the flask & immediately filter through the filtering cloth. Thoroughly wash the residue with

boiling water and transfer to a Gooch crucible prepared with a thin but compact layer of ignited

asbestos.

Wash the residue thoroughly first with hot water and then with about 15ml of 95% (V/V) ethyl

alcohol/ammonia solution.

Dry the Gooch crucible and contents at 105±1ºC in the air oven to the constant mass. Cool & wait.

Incinerate the contents of the Gooch crucible at 600±20ºC in a muffle furnace until all the

carbonaceous matter is burnt. Cool the Gooch crucible containing the ash in a desiccator and weigh.

15.1.4 Calculation:

Crude fiber (percent by mass) = (M1 – M2)100

m

Where:

M1 = mass in g of Gooch crucible and contents before ashing

M2 = mass in g of Gooch crucible containing asbestos and ash

m = mass in g of the dried material taken for the test.


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15.2 Determination of Crude Fiber by NIR Analyser:


16.0 DETERMINATION OF TOTAL ASH

16.1 Principle:

On heating of material at higher temperature at around 750ºC for a given period, the organic matter

will be decomposed and only metallic residue will remain in the residue. This residue is called ash.

16.2 Material:

Crucible

Desiccators

Muffle Furnace

16.3 Procedure:

Weigh accurately about 2 g of the sample in a tarred porcelain, crucible or platinum dish.

Complete the ignition by keeping in a muffle furnace at 750±20ºC until grey ash results.

Cool in a dessicator and weigh.

Ignite the dish again in the muffle furnace for 30 minutes, cool and weigh.

Repeat this process until the difference in the mass between two successive weighing is less than 1

mg. Note the lowest mass.

16.4 Calculation:

Total ash = 100 (M2 – M)

M

Where:

M2 = the lowest mass in g of the empty dish with the ash

M = Mass in g of the empty dish.

17.0 DETERMINATION OF ACID INSOLUBLE ASH (SILICA)

17.1 Determination of Acid Insoluble Ash by Chemical Method:

17.1.1 Principle:

On treatment of ash with dilute hydrochloric acid and on heating, the acid soluble ash will dissolved in

acid and rest will remain as residue. After filtration the insoluble ash can be separated.

17.1.2 Material:

(a) Crucible

(b) Desiccators


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Whatman filter paper no. 1

Muffle Furnace

17.1.3 Reagent:

(a) 1:4 Hydrochloric acid: Take 200ml AR grade HCl and make it 1 litre

17.1.4 Procedure:

Weigh accurately about 2 g of the sample in a tarred porcelain crucible or platinum dish.

Complete the ignition by keeping in a muffle furnace at 750±20ºC until gray ash results.

Cool and add 25 ml of dilute hydrochloric acid, heat on a water bath for about 3 hours.

Cool and filter through Whatman filter No. 1 or its equivalent. Wash the residue with hot water until

the washings are free from chlorides as tested with silver nitrate solution and return the filter paper

and residue to the dish.

Ignite it in a muffle furnace at 750±20ºC for one hour.

Cool in a desiccator and weigh.

Ignite the dish again for 30 minutes, cool and weigh.

Repeat this process till the difference two successive weighing is less than one milligram. Note the

lowest mass.

17.1.5 Calculation:

Acid insoluble Ash = 100 (M2 – M)

M

Where:

M2 = the lowest mass in g of the empty dish with the ash

M = Mass in g of the empty dish.

17.2 Determination of Acid Insoluble Ash by NIR Analyser:


18.0 DETERMINATION OF CALCIUM

18.1 Glassware: Volumetric Flask, pipette, beaker etc

18.2 Reagents:

Hydrochloric Acid: 25 ml of concentrated hydrochloric acid diluted to 100 ml.

Methyl Red Indicator: Dissolve 0.15 g of methyl red in 500 ml of water.

Ammonium Hydroxide Solution: 50 percent (V/V).

Dilute Ammonium Hydroxide Solution: 2 percent. (V/V).

Ammonium Oxalate Solution: saturated.


Page 24 of 36

Concentrated Sulphuric Acid: Sp. Gr. 1.84, Grade: AR

Sulphuric Acid (1:4): Take 200ml AR grade H2SO4 and make it 1 liter

(h) Saturated Potassium Permanganate Solution (0.1 N): Take 3.156g Potassium permanganate and

make it 1000ml with water.

18.3 Procedure:

Accurately weigh about 3 g of the material into a silica dish.

Char carefully and continue the ashing in a muffle furnace at a temperature not above 450ºC until the

ash is white or almost so.

Cool dish containing ash, moisten with a few milliliters of distilled water and add 3 to 5 ml of

concentrated hydrochloric acid drop by drop.

Evaporate to dryness on a water bath and continue heating on the water bath for one hour to render

silica insoluble.

Moisten the residue with 20ml distilled water and add about 2 to 3 ml of concentrated hydrochloric

acid.

Heat on a water-bath for a few minutes and filter through medium filter paper into a 250ml

volumetric flask. Wash the filter paper thoroughly with hot water, cool the filtrate and make it up to

volume (250ml), shake thoroughly.

Transfer a 25ml aliquot of the solution prepared as in (1) to a 400-ml beaker, dilute to about 100 ml

with water and add two drops of methyl red indicator solution.

Add ammonium hydroxide solution drop wise till a brownish-orange color is obtained (pH 5.6).

Add two drops of hydrochloric acid so that the color of solution is pink (pH 2.5 to 3.0).

Dilute to about 150 ml, bring to the boil and add slowly, with constant stirring, 10 ml of hot

ammonium oxalate solution. (If the red color of the solution changes to orange or yellow. Add

hydrochloric acid drop wise until the color again changes to pink).

Leave overnight to allow the precipitate to settle. Filter the supernatant liquid through ash less filter

paper and wash the precipitate thoroughly with dilute ammonium hydroxide solution.

Place the paper with the precipitate in the beaker in which precipitation was carried out and add a

mixture of 125 ml of water and 5 ml of concentrated sulphuric acid. Heat to 70 to 90ºC and titrate

with the standard potassium permanganate solution until the first slight pink color is obtained.

18.4 Calculation:

Calcium (as Ca) percent by mass = 2000 A N

m (100 – M)

Where:

A = volume in ml of the standard potassium permanganate solution required in the titration

N = normality of the standard potassium permanganate solution,


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m = mass in g of the material taken for the test and

M = percent moisture content.

19.0 DETERMINATION OF PHOSPHORUS

19.1 Glassware: Volumetric Flask, pipette, beakers, conical flask etc.

19.2 Reagents:

Concentrated Nitric Acid (1:1): Density 1.42 at 20ºC. A mixture of equal volumes of concentrated nitric

acid and water.

Indicator: Dissolve 0.15 g of methyl red in 500 ml of water.

Ammonium Molybdate Stock Solution – Take 200 g of powdered ammonium molybdate in a

stoppered graduated cylinder of 1000 ml. capacity, add to it 800 ml of water & shake well for 25

minutes to dissolve the ammonium molybdate. Add gradually 25 is clear (about 100 to 140 ml of

ammonium hydroxide may be required). Avoid adding excess of ammonia. Make up the volume to

one-liter .If necessary, filter the solution through a fluted paper and stock this solution.

Nitric Acid Solution: 2 percent (m/v)

Potassium Nitrate solution: 3 percent (m/v)

Standard Sodium Hydroxide Solution: 0.1 N

Standard Nitric Acid Solution: 0.1N

Phenolphthalein indicator Solution: Dissolve 0.1 g of Phenolphthalein in 100 ml of 60 percent (m/v)

rectified spirit.

19.3 Procedure:

A. Precipitation:

Take a 10ml aliquot of the prepared solution (As in Ashing and Extraction of Calcium) in a 150ml

beaker (Ref. Test Method: 1.9).

In a dry beaker, prepare ammonium molbdate solution by pouring into it, quickly and simultaneously

10 ml of the concentrated nitric acid first in the beaker and into this pour quickly 10 ml of the

ammonium hydroxide stock solution, whirling the beaker during addition.

Pour this freshly prepared clear liquid quickly into the beaker containing the aliquot and stir.

B. Filtration and washing:

Allow the precipitate to stand overnight and then filter through a disc of Whatman filter paper No. 42

in a gooch crucible by suction over or through a 9 cm Whatman filter No.42 over an ordinary funnel.

As far as possible only the supernatant liquid is passed through the filter paper, retaining the

precipitate in the beaker. When the supernatant liquid is decanted off, the precipitate is washed

twice with dilute nitric acid and then with potassium nitrate solution until the washings is free from

acid.


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If ordinary funnel and filter paper are used, freedom from acidity may be tested by collecting

sufficient filtrate in test tube to which a few drops of the standard sodium hydroxide solution are

added .If the pink color appears with one drop of the standard alkali, the precipitate is free from acid.

C. Titration:

Transfer the precipitate with the filter paper back to the beaker in which precipitation was carried

out. When gooch crucible is used for filtration, transfer the whole crucible along with the filter paper

to the beaker in which precipitation was carried out.

Add sufficient quantity of the standard sodium hydroxide solution from a burette just sufficient to

dissolve the precipitate and then add 5 ml in excess. See that no yellow precipitate sticks to the filter

paper.

Note the total volume of the standard sodium hydroxide solution added. Add about 10 drops of

phenolphthalein indicator solution and titrate the excess of alkali with the standard nitric acid.

19.4 Calculation:

Phosphorus (percent by mass) = 336.75(AN1 – BN2)

m(100 – M)

Where:

A = volume in ml of the standard sodium hydroxide solution used.

N1= normality of the standard sodium hydroxide solution,

B = volume in ml of the standard nitric acid used in to neutralize the excess alkali.

N2= normality of the standard nitric acid,

m = mass in g of the material taken for the test and,

M = percent moisture content.

20.0 DETERMINATION OF DENSITY

20.1 Density determination with conventional method:

20.1.1 Apparatus / Glassware:

Weighing balance: Capacity 5 kg with 2 decimal display

Measuring cylinder: Capacity one liter

20.1.2 Procedure:

Weigh the glass-measuring cylinder on the weighing balance.

Fill up the measuring cylinder with the finished product unto 1 liter mark.

Take the weight of material with cylinder.


Page 27 of 36

Calculate the density of the product (g/ml).

20.1.3 Calculation:

Density of the product (g/ml) = W2 – W1

V

Where:

W2 = Weight of the cylinder.

W1= Weight of the cylinder with material.

V = Volume of the product.

20.2 Determination of Density by Densitometer:

20.2.1 Apparatus / Glassware:

Density meter

Measuring cylinder: Capacity one liter

20.2.2 Procedure:

Arrange & set the densitometer assembly on the box.

Keep the one liter cylinder on the left side of the balance.

Set both sides of the balance in the equilibrium position.

Take out the Brass cylinder & fill it with the product.

Insert the cutter in the grooves of the one liter brass cylinder.

Remove the material from the upper part of the groove.

Again weigh it on the set balance.

Calculate the density of the product (g/ml).

20.2.3 Calculation:

Density of Molasses = Mass

Volume

21.0 UREA ADULTERATION TEST

21.1 Apparatus:

Glass Beaker: Capacity-250 ml.

21.2 Chemical:

1. Urea Strip:


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Weigh 2.5g urea powder with small amount of distilled water, stir into paste and dilute to 50ml with

distilled water.

Rub 0.15g bromothymol blue indicator powder in mortar with 2.4 ml 0.1N NaOH solution. After

indicator dissolves, wash mortar and pestle with distilled water and dilute to 50 ml. With distilled

Water. Solution should be green; pH approx. 7.0.

Mix solution (A) and (B) in equal proportion.

Take 90-ml mixture solution of (A) and (B) and mix with 10-ml glycerol. Pour mixture into watch glass.

Dip pieces of heavy filter paper (what man No.5) in solution. Hang papers to dry in place free from

NH3 fumes, strong air currents or heat. Paper should be orange when dry. Store dry papers in well

stopper glass bottle in a cool place. These strips will have a self-life of four months.

21.3 Procedure:

Take about 10 gm material in a 250 ml. Beaker.

Add 100 ml distilled water and mix well.

Take the urea strip & dip in the material mixed solution.

The strip turns green, which shows the presence of urea in the material.

22.0 DETERMINATION OF VITAMIN-A

22.1 Glassware:

Spectrophotometer

Hot air oven

Volumetric Flask: 10ml and 100ml

Hot Plate

Condenser

Separating Funnel

Conical flask with stopper

Beaker 250ml

Pipette: 1ml, 5ml and 10ml

22.2 Reagents:

(a) Chloroform

(b) Antimony tri Chloride (SbCl3)

(c) Rectified Spirit (Redistilled)

(d) Di Ethyl Ether

(e) Potassium Hydroxide: 40% (w/v)


Page 29 of 36

(f) Acetic anhydride

22.3 Procedure:

Weigh accurately about 0.5 g of the sample in a conical flask with stopper and add 7-8ml 40% KOH &

50ml Rectified Spirit (R Distilled).

Attach it with a condenser assembly & keep for 30 to 40 minutes for heating/refluxing.

After cooling the flask, add 30 ml distilled water and then add 50 ml of Di ethyl ether & shake well.

Transfer all the solution in a separating funnel and add twice 50 ml Di ethyl ether.

Shake well & filter it for three times, remove potassium Hydroxide by using glass distilled water

continuously & take out the lower portion, till the KOH in the solution gets completely removed.

Keep the flask in the hot water till the Di-ethyl ether gets evaporated. Afterwards keep the flask at the

low temperature hot plate & evaporate the aliquot.

1 ml aliquot in the 10ml measuring flask and make up the 10 ml solution with Chloroform.

Then take 1ml solution in 100ml volumetric flask & make up the 100 ml solution with chloroform

For blank reading: Take 3 ml. ChCl3 in a vial & add 2 ml. SbCl3 + 2 drops acetic anhydride. Take the

O.D reading from Colorimeter at 620 wavelengths.

Take 2 ml. ChCl3 in a vial & add 1 ml solution from 10 ml volumetric flask & add 2 ml. SbCl3 take the

O.D reading from Colorimeter at 620 wavelength & calculate the Vitamin-A.

22.4 Calculation:

Calculate the Vitamin A content from the standard curve using dilution factor.

23.0 DETERMINATION OF INFESTATION

Procedure:

Take 100 numbers of grains on a clean surface/table.

Count the infested grain(s) out of 100 grains.

Calculate infested grain(s) in percentage.

Calculation:

Infestation (%) = n X 100

N

Where:

n = Number of infested grain(s) in N numbers of grains

N = Number of grains taken for infested grain calculation/count

24.0 DETERMINATION OF DUST

Procedure:


Page 30 of 36

Take approximate 1 kg of grains.

Sieve in 1.00mm IS Sieve.

Weigh the sieved dust materials and calculate the dust as percentage.

24.2 Calculation:

Dust (%) = w x 100

W

Where:

w = Weight of dust materials

W = Weight of grains taken for dust analysis.

25.0 MOISTURE DETERMINATION OF MINERAL MIXTURE

25.1 Instrument:

1. Vacuum oven

2. Crucible

25.2 Procedure:

Weight accurately about 2 gm of the prepared sample in a silica dish.

Dry the sample in vacuum oven at 60 º Celsius and 20” Hg pressure for a minimum of 2 hours.

Keep the silica dish in desiccator to cool.

Take weight and calculate for moisture content.

25.3 Calculation:

Moisture (%) (by mass) = 100 (M1 – M2)

M1 – M

Where,

M1 = mass in gm of the dish with material before drying

M2 = mass in gm of the dish with material after drying, and

M = mass in gm of the empty dish

26.0 MESH SIZE DETERMINATION OF CALCITE POWDER

26.1 Material:

1MM IS Sieves

26.2 Procedure:

Take 1kg of sample and sieves through the 1MM IS sieve.

Check for any retained quantity on the sieve.

The 100% sample should passes through the sieve.


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26.3 Calculation:

Retained Quantity (%) = w X 100

W

Where:

w = Weight of retained quantity on the sieve.

W = Weight of sample taken for testing

27.0 PURITY DETERMINATION OF GRINDING SALT

27.1 Glassware:

250ml conical flask

100ml volumetric flask

10ml graduated pipette

10ml volumetric pipette

Reagent:

0.1N AgNO3: Take 16.9870 gm AgNO3 and make 1000 ml with glass distilled water. Keep in dark color

bottle & store at dark place.

Potassium dichromate indicator: 5% solution in distilled water.

Procedure:

Take 1.0gm salt sample in a beaker and dissolve it in glass distilled water. Make it 100ml in 100ml

volumetric flask.

Take 10ml of solution in conical flask and add potassium dichromate indicator to the solution (the

solution will be yellow).

Titrate it with 0.1N AgNO3 to the end point break red colour.

Take the final volume of 0.1N AgNO3 for calculation.

27.4 Calculation:

Purity of Salt (%) = R x 0.585 x10

Where: R = Burette reading in ml

28.0 TESTING OF ‘A’ TWILL GUNNY BAGS

28.1 Moisture Determination of Gunny bags:

28.1.1 Apparatus:

IJIRA moisture meter.

28.1.2 Method:

IJIRA Moisture meter may be used for this purpose. This meter works on the principle of measuring

the electrical resistance/conductivity of jute materials, which changes with moisture content in the


Page 32 of 36

material. Before using the meter brush the dial of the meter, the bottom surface of the electrode, the

plug pins.

Put the main switch at the bottom of the panel to the “ON’ position and throw the scale –selector

Switch (the upper one) the position L,(this indicates that the readings should be recorded on the

“L”(outer)scale of the meter

Adjust the “Y” (right hand control to bring the pointer in line with the mark “y” (at the right hand end

of the scale).

Press the white “push” switch and adjust the “X” (left hand) control to bring the pointer in line with

the mark “X”(at the left hand of the scale) and release the “push” switch.

To check calibration, insert the resister into the electrode socket and observe the meter reading,

which should correspond to the value specified on the resister .If it does not correspond, the meter is

not in order and should not be used.

Place the electrode on the material under test in such a way that the entire area of the electrode face

is in contact with the sample and apply pressure gradually on the electrode until the meter indicates

the present regain of the material.(avoid the pressure ,since this may damage the spring )

If the pointer reads beyond the scale “L” change the scale selector switch to the position “H” and

reset adjustments (as above),if required and carry out the test.

28.2 Length and width (Dimension):

Lay the bag flat on a table free from creases and wrinkles and measure the outside length and outside

width about the center of the of the bag to the nearest 0.5cm.Determine the average length and

width of the bags under test

28.3 Mass:

Weigh each bag to the nearest 5 g after tests for B-1 and B-2Number of bags and joined bags per bale.

28.4 ENDS AND PICKS:

Count the ends and picks from each bags in one and two places respectively with a suitable gauge

measuring 10 cm. Determine the average and picks per decimeter of the bag under test in accordance

with 7 of IS: 1963-81.

28.5 Corrected net weight:

Corrected net weight of a bale is calculated as follows:

Corrected net weight of a bale = Net weight x (100 + contract regain, percent)

---------------------------------------------------------------

100 + Average moisture regain

29.0 TESTING OF HDPE BAG

29.1 Size:


Page 33 of 36

Lay the bag flat on a table free from creases and wrinkles and measure the outside length and outside

width about the center of the bag to the nearest 0.5mm. Determine the average length and width of

the bags under test it should be 24”X45” (tolerance +-10mmX+- 15 mm.

29.2 Mass:

Weigh each bag to the nearest 5 g after tests.

Weight: Put the HDPE bag on the balance and record the weight.

29.3 Printing: As per our monogram .It should have single color.

29.4 Tensile strength will be checked on a random basis (70 kg.) wart and weft. Or according to

the size of bag .

30.0 TESTING OF WATER

30.1 Sampling: Sampling is done in cleaned beaker from different bore well and from other sources

requires to chemical and / or bacteriological analysis. For bacteriological analysis of water, sterile

glass containers use for sampling. For chemical testing of water, approximate 500ml of sample is

collected and for bacteriological analysis, approximate 200ml of sample is collected (Sample quantity

is as per QP-01).

30.2 Determination of Total Hardness of Water:

Take 100 ml of water sample in a glass beaker.

Add 1 ml Ammonia buffer solution.

Add 3 to 4 drops of erichrome black – T solution.

Titrate it with 0.02 N EDTA solutions till colour changes from pink to blue.

Calculation:

Total Hardness in PPM = Reading in ml used for titration X 10

30.3 Determination of Total Dissolve Solids (TDS) of Water:

Take 50ml of filtered water sample in an aluminum pre-dry and pre-weighted (W1) dish.

Place the dish on hot plate and keep it till evaporates the water from the dish.

Place the dish in hot air oven maintained at 100±1ºC for 30 minutes.

Transfer the dish in dessicator.

After cooling, take weight of the dish (W2) and calculate TDS.

Calculation:

TDS (in ppm) = (W2-W1) x1000x1000

Volume of Sample (50 ml)

30.4 Determination of pH:


Page 34 of 36

pH value can be determined either by pH meter or the indicator method. It should be

determined as early as possible after the sample is collected as sample kept for a long time are likely

to change in pH due to escaping of dissolved gases such as carbon dioxide.

31.0 DETERMINATION OF CRUDE PROTEIN BY SEMI AUTOMATIC EQUIPMENT (EITHER KEL-PLUS OR

BIOKJEL)

31.1 Principle: The crude protein can be determined by pelican’s KEL-PLUS system, which is an

improved semi-automated method of analysis.

31.1.2 Glass wares:

Block digestion system (Kelplus KES 06L) - Heating block, insert rack, manifold rack, support rack

for insert rack, support rack for manifold rack, exhaust manifold, filter pump.

Distillation apparatus (Kelplus-Elite ex)

Scrubber unit (Kelplus-kelvac)

31.1.3 Reagents:

(a) Potassium Sulphate

(b) Copper Sulphate

Concentrated Sulphuric Acid

Sodium Hydroxide Solution (20%)

Sodium Hydroxide Solution (40%)

4% Boric acid solution

(f) 0.1 N Sodium Hydroxide Solution

(g) Mix Indicator: 0.3 g Bromocresol green & 0.2g Methyl red in 400 ml of 90% Ethyl alcohol

31.1.4 Procedure:

Weigh the sample in a digital precision balance and transfer it into the digestion tube.

Add digestion mixture to the sample. Also add required volume of concentrated H2SO4 into the tube

Using SS insert rack, take samples to the digestions systems

Manifold are permanently connected to Kelvac. Place the manifold with manifold rack over the

digestion tube. The water inlet to Kelflow is connected to water tap.

Load the insert rack with tubes and manifold into the block digestion system. Open the water tap for

switch on the Kelvac system. Ensure proper sealing of exhaust manifold to avoid leakage of fumes

Switch on the power switch. Set the temperature in the temperature controller. If frothing is

observed in the sample, digest the sample in low temperature for some time and increase the set

temperature.


Page 35 of 36

After the digestion of samples is over, separate the sample from the system. Remove the insert rack

with samples and place it over the tube support rack. Allow water to flow through the Kelflow for

sometime, to remove the fumes from the tube.

Remove the manifold from the tubes. Place the manifold in the manifold support rack.

Take out the sample one by one and dilute with distilled water. Dilution is necessary to avoid

solidification of sample on cooling. The diluted sample is placed in the distillation system for ammonia

recovery.

Keep the alkali, reservoirs to the rear of the system. Immerse the hose coming out from the system

into the alkali tank

Wait for the READY indication in the control panel. Open the water inlet to the water condenser.

Select the volume and alkali to be added using selector switch and press the alkali switch for alkali

addition.

Select the distillation time by using selector switch and press process switch for steam addition.

Ensure addition of reagents and steam. Reagents can also be added manually.

Water refilling to the boiler takes place automatically. During refilling of water ensure an alarm and an

indication. Ensure the distilled water reservoir always filled with sufficient distilled water. Ensure

collection of ammonia in the receiver solution.

Remove the receiver solution with ammonia and take to a auto titrator / manual burette for titration

Titrate either by direct or back titration to estimate the nitrogen percentage

31.1.5 Calculation: % Nitrogen = 14 x Normality of Acid x (TV-BV) x 100

Weight of sample x 1000

Where: TV = Titration value, BV = Blank value

% Protein = % Nitrogen x 6.25

Note: Procedure to work with BIOKJEL Semiautomatic equipment is same as of KEL PLUS.

DETERMINATION OF MOISTURE BY AXIS LCGC ELECTRONIC MOISTURE ANALYSER

Moisture in substances is an essential quality factor of technical and economical importance.

Methods of determining moisture may be grouped in two main categories: absolute and deductive.

Absolute methods are based on simple relations, e. g. weight decline during drying.

Thermogravimetric analysis used in AXIS LCGC Moisture Analyser

Thermogravimetric Analysis:

A process of determination of a substance mass decline as a result of heat-up. The sample is weighed

before and after heating-up, the difference is calculated in relation to initial weight or final weight

(dry mass).

Moisture in substances:


Page 36 of 36

Thermogravimetric analysis includes all ingredients evaporating from substances during heating-up,

which results in weight decrease.

Final equation for Moisture %

Moisture % = Mo – M /M * 100

Where,

Mo = Initial weight, M = current weight

~ END OF TENDER DOCUMENTS ~