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Megamecia
IntroductionModel Organism
Paramecium TetraureliaMutagen
Ultraviolet Radiation
Paramecium Tetraurelia
Ultraviolet RadiationElectromagnetic Spectrum
100 nm – 400 nm range
Causes pyrimadine dimers to form in DNA (Goodsell, 2001)Pyrimadine dimers may result in the formation of a point
mutation.
http://www.uvsciences.com/uvtech.html
Pyrimadine DimersCovalent bond between two adjacent pyrimadine
bases (Thyamine, Cytosine) Point Mutation – Transition Mutation
Switching of complementary base pairs A-T becomes G-C (Russell, 2010)
http://www.mun.ca/biology/scarr/F16-20,25.jpg http://www.uvsciences.com/images/dna.jpg
Methods500 μL culture samples added to tissue culture traySamples exposed to UV radiation in a UV chamber.
Exposed between 10 and 60 minutes in 10 minute intervals
MethodsCulture trays wrapped in tin foil immediately
after exposure.Paramecia have the ability to repair DNA while
exposed to white lightRepeated 30 minute exposure with new
samples200 μL klebsiella inoculated wheat media added
to each sample after exposureSamples checked periodically for changes
Photos taken using a digitial microscope and Applied Vision
Measurements done with Image J
RESULTS30 minutes was the
optimum time to keep the cells in UV light
Mutant cells were 22% larger in length & 15% larger in width
RESULTSMUTANTS CONTROL
DISCUSSIONOther mutations that weren’t able to be cultured
DISCUSSIONExperiment could be taken further
Analyze DNA to pinpoint where mutation occurred
Experimental errorsTissue culture trays should have been checked
for cells before exposure to Ultraviolet radiation
Ultraviolet radiation successfully caused a mutation
REFERENCESGoodsell, D.S. (2001). The Molecular Perspective: Ultraviolet Light and Pyrimidine Dimers. The Oncologist, 6(3), 298-
299. Retrieved from http://theoncologist.alphamedpress.org/cgi/content/full/6/3/298
Russell, P.J. (2010) iGenetics: A Molecular Approach 3rd Ed. San Francisco, CA: Pearson Education, Inc.