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MEDICAL MYCOLOGY
MEDICAL MYCOLOGY COURSE
(from 21st March to 15th April, 2016)
Co-directors
Head of studies
Dea GARCIA HERMOSO
UNITE DE MYCOLOGIE MOLECULAIRE INSTITUT PASTEUR
INSTITUT PASTEUR EDUCATION CENTER
Pavillon Louis Martin - Bâtiment No 9C
28, rue du Docteur Roux
75724 PARIS Cedex 15 (France)
Lectures: Room 2 on the ground floor
Practical work: on the 2nd
floor
Françoise DROMER Olivier LORTHOLARY
GENERAL PRESENTATION This four-week course is intended for microbiologists (MD, PhD, VetD) practicing mycology in hospital laboratories to catch up with the most recent advances in diagnosis and identification procedures and principles of therapy for fungal infections. Training is provided through bench works, lectures and panel discussions and will cover: Technical aspects : choice of media, slide cultures, storage of isolates, molecular diagnosis and identification (PCR, qPCR, sequencing, MALDI-TOF), typing techniques (microsatellite, MLST), antifungal susceptibility testing (CLSI/EUCAST microbroth dilution methods, commercially available tests), antigen/antibody detection, histopathology. Practical aspects : spectrum of action and pharmacokinetics/dynamics of antifungal drugs, role of the laboratory in the diagnosis and monitoring of patients (direct examination, histopathology, antigen detection, antifungal susceptibility testing results, qPCR …), limitation and use of public and specific databases for molecular identification, discussion of clinical cases. Fungal aspects : sexual and asexual reproduction, phylogeny, species identification, resistance mechanisms. Medical aspects : Pathogenic concepts, epidemiology, diagnosis criteria for infections due to common and emerging yeasts or filamentous fungi (including invasive and mucocutaneous infections, endemic mycoses), principles of therapeutic management, practical guidelines.
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ALL PRACTICAL SESSIONS WILL BE SUPERVISED BY DEA GARCIA-HERMOSO WITH THE TECHNICAL HELP OF MURIELLE ALMOUSSA AND GUYLENE K’OUAS
FIRST WEEK
Monday, 21st March
8:30 - 9:00 Introduction to the course Françoise DROMER, Dea GARCIA HERMOSO Olivier LORTHOLARY
(Institut Pasteur, France)
9:30 - 11:00 Student reception Registrar’s Office, Secretary to the course Muriel ALMOUSSA, Guylène K’OUAS (Institut Pasteur, France)
11:00 - 13:00 Basic techniques (lecture & bench work) Françoise DROMER, Dea GARCIA HERMOSO (Institut Pasteur, France)
14:00 - 17:00 Basic techniques (bench work) 17:00 – 18:30 Mass spectrometry Alexandre ALANIO
Tuesday, 22nd March
9:00 – 13 :00 Sexual reproduction (lecture + bench work) Joëlle DUPONT (Museum National d’Histoire Naturelle, France)
Dea GARCIA HERMOSO (Institut Pasteur, France)
14:00 - 18:00 Asexual multiplication (lecture + bench work) Alberto STCHIGEL (Medical School Rovira i Virgili University, Spain)
Wednesday, 23rd March
9:00 - 12:30 Asexual multiplication 2 (bench work) Alberto STCHIGEL (Medical School Rovira i Virgili University, Spain)
Dea GARCIA HERMOSO (Institut Pasteur, France)
13:30 - 15:30 Yeast Identification Procedures (lecture) Jacques BILLE (Univ. Hospital Lausanne, Switzerland)
15:30 - 18:00 Invasive Candida spp infections (lecture) Thierry CALANDRA (Centre hosp. Universitaire Vaudois, Switzerland)
Thursday, 24th March
9:00 - 12:30 Keys of Aspergillus identification Paul VERWEIJ, Ton RIJS, Henrich VAN DER LEE
(lecture + bench) (University Hospital Gasthuisberg, Belgium)
13:00 - 15:30 Yeast Biology (lecture) Teun BOEKHOUT (CBS-KNAW Fungal Biodiversity Centre, The Netherlands)
16:00 - 18:30 Yeast (1) : bench work Françoise DROMER, Marie DESNOS-OLLIVIER (Institut Pasteur, France)
Friday, 25th March
9:00 - 13:00 Molecular Biology (yeast and molds): bench work Dea GARCIA HERMOSO Murielle ALMOUSSA
Guylène K’OUAS (Institut Pasteur, France)
14:00 - 14:30 Gel deposit
14:30 - 16:30 Histology of normal tissue : lecture Fabrice CHRETIEN, Gregory JOUVION (Institut Pasteur, France)
16:30 - 18:15 MALDI : bench work Marie DESNOS-OLLIVIER, Dea GARCIA HERMOSO (Institut Pasteur, France)
18:15 – 18:30 Course assessment to fill out
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SECOND WEEK
Tuesday, 29th March 8:30 - 10:30 Antifungal susceptibility testing : lecture Maiken ARENDRUP (Statens Serum Institut, Denmark)
10:30 - 12:00 Antifungals : bench work 1 Françoise DROMER, Marie DESNOS-OLLIVIER (Institut Pasteur, France)
13:00- 15:15 Molecular biology practical approaches : lecture Stéphane BRETAGNE (Institut Pasteur, France)
15:30 - 17:00 Antifungal drugs / mode of action / Vincent JULLIEN pharmacokinetics-dynamics : lecture (Hôpital Georges Pompidou, France)
17:00 - 18:30 Project distribution Françoise DROMER, Dea GARCIA HERMOSO (Institut Pasteur, France)
Wednesday, 30th March 9:00 - 11:00 Invasive and chronic aspergillosis : lecture Johan MAERTENS (University Hospital Gasthuisberg, Belgium)
11:30 - 13:00 Environmental fungal risk : from the hospital setting Jean-Pierre GANGNEUX
to home exposure : lecture (CHU Pontchaillou de Rennes, France)
14:00 - 15:30 Molecular biology new techniques NGS : lecture Stéphane BRETAGNE (Institut Pasteur, France) 16:00-18:00 Immunity to fungi Jessica QUINTIN (Institut Pasteur, France)
Thursday, 31st March
8:00 - 9:30 Genome organization of fungi : lecture Christophe D’ENFERT (Institut Pasteur, France)
9 :30-11 :15 Antifungal discussion : bench work 2 Françoise DROMER, Marie DESNOS-OLLIVIER
11:30 - 13:00 Time for project Dea GARCIA HERMOSO
Muriel ALMOUSSA, Guylène K’OUAS 14:00 - 16:00 Barcoding and sequence databases : lecture Vincent ROBERT (CBS-KNAW Fungal Biodiversity Centre, The Netherlands)
16:30 - 18:00 Flow chart of yeast and molds identification Dea GARCIA HERMOSO
Friday, 1st April 9:00 - 10:30 Mucormycosis and Entomophthoromycosis : lecture Fanny LANTERNIER (Hôpital Necker-Enfants Malades, France)
11:00 - 13:00 Mucorales 1 : lecture & bench work Dea GARCIA HERMOSO 14:00 - 16:30 Mucorales 2 : lecture & bench work Dea GARCIA HERMOSO 16:30 – 16:45 Course assessment to fill out 16:45-18:30 Time for project Dea GARCIA HERMOSO
Muriel ALMOUSSA, Guylène K’OUAS
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THIRD WEEK
Monday, 4th April
9:00 - 12:00 Emerging molds infections and pneumocystosis : lecture Patricia MUNOZ (Hospital General Universitario G. Maraňon, Spain)
13:00 - 17:00 Emerging molds : lecture & bench work1 Dea GARCIA HERMOSO
17:00 - 18:30 Time for project Dea GARCIA HERMOSO Muriel ALMOUSSA, Guylène K’OUAS
Tuesday, 5th April
9:00 - 13:00 Emerging molds : bench work 2 Dea GARCIA HERMOSO
14:00 - 15:00 Pneumocystis : lecture Alexandre ALANIO (Institut Pasteur & Hôpital Saint-Louis, France)
15:15- 18:15 Emerging molds : bench work 3 & sequence analysis Dea GARCIA HERMOSO
Wednesday, 6th April
9:00 – 10:45 Genotyping methods for fungi : lecture & practical discussion Stéphane BRETAGNE
11:00 - 13:00 Antifungal drug resistance - mechanism : lecture Dominique SANGLARD (Université de Lausanne, Switzerland)
14:00 - 16:30 Endemic mycoses : lecture Olivier LORTHOLARY (Institut Pasteur, France)
16:30 - 18:30 Dimorphic fungi and diagnosis of endemic mycosis Manuel CUENCA ESTRELLA (Centro Nacional de Microbiologia, Spain)
Thursday, 7th April
9:00 - 11:00 Animal mycoses : lecture Jacques GUILLOT (Ecole Nat. Vétérinaire d’Alfort, France)
11:30 - 13:00 Histopathology : lecture & bench work Fabrice CHRETIEN, Gregory JOUVION (Institut Pasteur, France)
13:30 - 15:30 Histopathology : lecture & bench work Fabrice CHRETIEN, Gregory JOUVION
16:00 - 18:30 Time for project Dea GARCIA HERMOSO Muriel ALMOUSSA, Guylène K’OUAS
Friday, 8th April
9:00 - 10:30 Time for project Dea GARCIA HERMOSO Muriel ALMOUSSA, Guylène K’OUAS
10:30 - 13:00 Cutaneous and mucosal infections (Candida/Malassezia) Roderick HAY (International Foundation of Dermatology, UK)
14:00 - 15:30 Cryptococcosis : lecture Olivier LORTHOLARY 15:30 – 15:45 Course assessment to fill out
16:00 - 18:30 Serological tools : lecture Olivier LORTHOLARY, Stéphane BRETAGNE
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FOURTH WEEK
Monday, 11th April 9:00 - 10:30 Time for project Dea GARCIA HERMOSO
Muriel ALMOUSSA, Guylène K’OUAS
11:00 - 13:00 Dermatophytes : lecture Malcolm RICHARDSON (University Hospital of South Manchester, UK)
14:00 - 18:00 Dermatophytes : bench work Malcolm RICHARDSON
Tuesday, 12th April 9:00 - 13:00 Revision / Projects 14:00 - 15:30 Fungal cell wall : lecture Jean-Paul LATGE (Institut Pasteur, France)
16:00 – 18:30 Revision
Wednesday, 13th April 9:00 - 12:00 Revision / Projects
13:30 - 18:30 Projects / Exam
Thursday, 14th April 9:00 - 11:30 Written exam
(See detailed description next page)
Friday, 15th April 11:00 - 12:30 Debriefing Committee
13:00 - 16:00 Debriefing students 16:30 - 18:00 Brindis
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EXAMINATION
MEDICAL MYCOLOGY COURSE The final mark will include 3 components: 1) Written exam On Thursday, 14th April (duration 2 hrs 30): mark on 1-20 scale, coefficient 1 Accepted language: English, French No document allowed in the classroom 2) Oral exam: (1-20 scale, coefficient 1) On Wednesday, 13th April On March 29th a draw will be organized. Each student will get an unknown yeast or mould isolate. Each isolate will be associated with a clinical history. In the following 3 weeks, the student will work to achieve identification to the species level, and will present it on the day of the exam. Each student will prepare a short Powerpoint file to present the entire procedure and the key features allowing identification, including morphology (pictures welcome), differential diagnosis and key elements of the clinical history. On April 12th there will be another draw to decide the order of the presentations. The powerpoint presentation should be handled to the Head of Studies on Wednesday 13th April, before 11 pm. The oral presentation (10 min max) in English (with slides in English) will be performed in front of all students and a committee composed of the Head of Studies and the course directors. Questions will be asked by the committee and the fellow students. 3) Participation in bench work, student’s investment, quality of work, participation in discussions will be evaluated throughout the duration of the course by the Head of Studies and the course directors (1-20 scale; coefficient 0.5).
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MEDICAL MYCOLOGY COURSE
2015 - 2016
CO-DIRECTORS
Mrs Françoise DROMER, MD, PhD Unité de Mycologie Moléculaire, Institut Pasteur +33-(0)1 40613389 / +33-(0)682071073 25/28, rue du Dr Roux email : [email protected] 75724 Paris Cedex 15 France
Mr Olivier LORTHOLARY, MD, PhD Unité de Mycologie Moléculaire, Institut Pasteur +33-(0)1 45 68 87 39 25/28, rue du Dr Roux email : [email protected] 75724 Paris Cedex 15 France
and
Service des Maladies Infectieuses et Tropicales Hôpital Necker-Enfants malades, Université Paris Descartes Centre d'Infectiologie Necker-Pasteur, IHU Imagine 149, rue de Sèvres, +33-(0)1 42 19 26 63
75743 Paris Cedex 15
HEAD OF STUDIES
Mrs Dea GARCIA HERMOSO, PhD Unité de Mycologie Moléculaire, Institut Pasteur +33-(0)1 40613341 25/28, rue du Dr Roux email : [email protected] 75724 Paris Cedex 15 France
LECTURERS
Mr Alexandre ALANIO Unité de Mycologie Moléculaire Institut Pasteur +33-(0)1 40 61 32 55 25/28, rue du Dr Roux email : [email protected] 75724 Paris Cedex 15 France
and
Laboratoire de Mycologie-Parasitologie Hôpital Saint-Louis +33-(0)1 42 49 95 02 1, avenue Claude Vellefaux email : [email protected] 75475 Paris Cedex 10 France
Mrs Maiken Cavling ARENDRUP Head of Unit of Mycology Statens Serum Institut email : [email protected] Artillerivej 5 2300 Copenhagen Denmark
Mr Jacques BILLE Honorary Professor in Clinical Microbiology email : [email protected] University Hospital Lausanne Switzerland Mr Teun BOEKHOUT CBS-KNAW Fungal Biodiversity Centre email: [email protected] Uppsalalaan 8 CT Utrecht The Netherlands
Mr Stéphane BRETAGNE Unité de Mycologie Moléculaire Institut Pasteur +33-(0)1 45 68 83 55 25/28, rue du Dr Roux email : [email protected] 75724 Paris Cedex 15 France
and
Hôpital Henri Mondor Laboratoire de Parasitologie/Mycologie Faculté de Médecine 8, avenue du Général Sarrail email : [email protected] 94010 Créteil Cedex France
Mr Thierry CALANDRA Département de Médecine Service des Maladies Infectieuses Centre Hospitalier Universitaire Vaudois email : [email protected] Rue Bugnon 46 1011 Lausanne Suisse Mr Fabrice CHRETIEN Unité d’Histopathologie humaine et Modèles animaux Institut Pasteur +33-(0)1 40 61 31 44 25/28, rue du Dr Roux email : [email protected] 75724 Paris Cedex 15 France
Mr Manuel CUENCA-ESTRELLA Centro Nacional de Microbiologia Spanish National Center for Microbiology +34-91 8223909 Ctra. Majadahonda-Pozuelo Km2 email : [email protected] Majadahonda, Madrid 28220 Spain
Mr Christophe D’ENFERT Unité Biologie et Pathogénicité Fongiques INRA USC2019 +33-(0)1 40 61 32 57 Institut Pasteur email : [email protected] 25/28, rue du Dr Roux 75724 Paris Cedex 15 France
Mrs Marie DESNOS-OLLIVIER CNRMA - Unité de Mycologie Moléculaire Institut Pasteur email : [email protected] 25/28, rue du Dr Roux 75724 Paris Cedex 15 France
Mrs Joëlle DUPONT Museum National d’Histoire Naturelle Département Systématique et Evolution email : [email protected] 57, rue Cuvier – CP 39 75231 Paris Cedex 05 Mr Jean-Pierre GANGNEUX Parasitology – Mycology Centre Hospitalier Universitaire de Rennes INSERM U1085 email : [email protected] 2, rue Henri Le Guillou 35033 Rennes Cedex 09 France
Mr Jacques GUILLOT Unité de Parasitologie, Mycologie, Dermatologie EA Dynamyc, UPEC, EnvA Ecole Nationale Vétérinaire d’Alfort email : [email protected] 7, avenue du Général de Gaulle 94704 Maisons-Alfort France
Mr Roderick J. HAY Chairman, +44-207 3886515 International Foundation for Dermatology email : [email protected] William House, 4 Fitzroy Square London W1T 5HQ UK
Mr Gregory JOUVION Human Histopathology and Animal Models Institut Pasteur +33-(0)1 45 68 86 61 25/28, rue du DrRoux email [email protected] 75724 Paris Cedex 15 France
Mr Vincent JULLIEN Service de Pharmacologie Hôpital Européen Georges Pompidou +33-(0)1 56 09 39 77 20, rue Leblanc email [email protected] 75015 Paris France
Mrs Fanny LANTERNIER Service de Maladies Infectieuses & Tropicales Centre d’Infectiologie Necker-Pasteur +33-(0)1 44 49 44 29 Hôpital Universitaire Necker – Enfants Malades email [email protected] 149, rue de Sèvres 75015 Paris France
Mr Jean-Paul LATGE Unité des Aspergillus Institut Pasteur +33-(0)1 40 61 35 18 25/28, rue du Dr Roux email : [email protected] 75724 Paris Cedex 15 France
Mr Johan MAERTENS University Hospital Gasthuisberg Leuven Department of Hematology email : [email protected] Herestraat 49 3000 Leuven Belgium
Mrs Patricia MUNOZ Servicio de Microbiologia Clinica y Enfermedades Infecciosas, CIBERES - Hospital General Universitario Gregorio Maranon email : [email protected] Dr Esquerdo 46 28007 Madrid Spain
Mrs Jessica QUINTIN Groupe Immunologie des Infections fongiques Institut Pasteur +33-(0)1 44 38 94 54 25/28, rue du DrRoux email : [email protected] 75724 Paris Cedex 15 France
Mr Malcolm RICHARDSON Mycology Reference Centre Manchester Education and Research Centre University Hospital of South Manchester +44-1 612915914 (Wythenshawe Hospital) email : [email protected]
Manchester, M23 9LT UK
Mr Vincent ROBERT CBS-KNAW Fungal Biodiversity Centre +31-(0)32 478 28 57 64 Uppsalalaan 8 email : [email protected] 3584 CT Utrecht The Netherlands
Mr Dominique SANGLARD Institute of Microbiology University of Lausanne & University Hospital Center +41-(0)21 31 44 083 Rue Bugnon 48 email : [email protected] 1011 Lausanne Switzerland Mr Alberto M. STCHIGEL Medical School Rovira I Virgili University +34-(0)977 759 341 Mycology Unit email: [email protected] C/Sant Llorenç 21 Reus Spain Mr Paul E. VERWEIJ Professor of Medical Microbiology +31-(0)24 3614 356 Radboud University Nijmegen Medical Center email: [email protected] P.O Box 9101 6500 HB Nijmegen The Netherlands
PRACTICAL WORK Mrs Murielle ALMOUSSA Centre d’Enseignement Institut Pasteur +33-(0)1 45 68 85 00 25/28, rue du Dr Roux email [email protected] 75724 Paris Cedex 15 France
Mrs Guylène K’OUAS Centre d’Enseignement Institut Pasteur +33-(0)1 4 25/28, rue du Dr Roux email [email protected] 75724 Paris Cedex 15 France
ADMINISTRATIVE ASSISTANT
Mrs Chantal MARTINEZ Centre d’Enseignement Institut Pasteur +33-(0)1 45 68 82 91 25/28, rue du Dr Roux email [email protected] 75724 Paris Cedex 15 France
