Medical InterventionsHewitt-Trussville High SchoolSeptember 28,
2011Bacterial Conjugation LabObjectiveDiscuss the
following:Bacterial conjugationAntibiotic resistanceLab
ProcedureStreakingAseptic TechniquePerform antibiotic resistance
confirmationPredict results of resistancePneumococcusBacteria found
in the back of the nose of a healthy personWhen it moves, it can
cause ear infection, sinus infection, pneumonia, and
meningitisBefore 1940, a pneumococcus infection was deadly.What
changed?What has this done to the bacteria over time?
http://textbookofbacteriology.net/S.pneumoniae.htmlGenetic
Recombination of
BacteriaTransformationTransductionConjugationhttp://microculture.tumblr.com/post/6293909843/fyeahmedicine-bacterial-conjugation-method-of
ConjugationPlasmidExtra chromosomal DNADouble stranded and
circularContain from 3 to 25 genesF (fertility) factorFound in E.
ColiRegulates formation of pili
Prep WorkMelted LB agarLB agar = Lysogeny brothNutrient rich
medium for bacteria to growAdded antibiotics to melted agarFirst =
added streptomycin (LB + str)Second = added ampicillin (LB +
amp)Third = added both antibiotics (LB + str + amp)Created the E.
coli broths from the slantStrain I = Streptomycin ResistantStrain
II = Ampicillin ResistantDirectionsWipe down the desk with the
cleaning wipesCollect four agar platesLBLB + strLB + ampLB + str +
ampWrite your initials on the plateDraw a line down the middle of
the bottom side of the plateLabel one side as ILabel the other side
as IIDo this for all 4 platesAseptic
Techniquehttp://www.youtube.com/watch?v=tBmNitxvqyc&safety_mode=true&persist_safety_mode=1DirectionsPlace
the loop with the Strain I directly under where you labeled the
IStreak the plate as
shown.http://faculty.ccbcmd.edu/courses/bio141/labmanua/lab2/streak_an.htmlComplete
these same steps for Strain II on the other side of the
plate.Complete this for all four plates.IIIIIIIIIDirectionsAllow
the liquid to soak into the agar.INVERT the plates and place in a
stack of four in the incubatorIn the prep roomWipe all of the desk
down with the wipesWash handsComplete the chart.
***Place all used inoculation loops into the biohazard bag in
the prep roomDO NOT THROW THESE AWAY!Data ChartAgar PlatesE. Coli
Strain I Predicted ResultsE. Coli Strain II Predicted ResultsLB
onlyLB + strLB + ampLB + str + ampMaterials4 Disposable inoculation
loopsApronsGogglesGlovesBunsen Burner4 Agar plates (LB, LB+str,
LB+amp, LB+str+amp)2 E. Coli broths (Strain I and Strain II)
