MECCR-Multiple Environment Cell Culturing Reactor

Group 2

Andrew Buchanan, Mary Alice Klenk, Matteo McKinney, Henry Pham, Gabriela Ramsey, Austin Sharp, Mohammed Taha, Andrea Wright

70”

Material Transport• 0.0006” step size for rack

• Proximity sensor for

safety

• NEMA-17 motor drives

vertical screw

Cap Remover• Spring powered bracket

to grab caps

• NEMA-23 to twist off cap

Liquid Dispensing• NEMA-17 motor drives

screw

• Disposable pipette tip

User Interface• Raspberry Pi and

Smoothie board

• 407.47 total Watts

Structure/Enclosure• 388.32 lb overall weight

• BSL-2 Biosafety Cabinet

Climate Control• 2 well plate, 2 conical

tube containers

• 5 gases controlled

through automatic valves

• 4-70℃ in 2.36 minutes

Measurement• 3 distinct devices, Non-

384, 384, tube

• NEMA-17 motors power

belt

• Maximum 0.5 seconds to

measure well plate

Plate Movement• Linear, orbital, double

orbital

• NEMA-17 motors: linear

screw, circular arm

• Rubber pads for vibration

dampening

Passionate about

Best at Economic Engine

• Passion about: Designing high

quality autonomous bioreactors

• Best at: Culturing cells in a

controlled environment for as

long as possible

• Economic Engine: Reduce

cost for more laboratories to

afford

Measurement

LEDs• 3mm diameter

• 600 nm

• 16 count

• immediate turn-on

Photodiodes• 16 count

• 2 ms response time

NEMA 17

• 1000 rpm max speed

• 1.8° rotation per pulse

Well Plate ~ 127.76 mm long

0.383284s to move panel across

the well plate

Total Measurement Time @ max

speed (500 rpm) ~ 0.50328s

Diode Array• 384 count

OD/FI 384 Well Plate

8.07"

• 4.5 mm separation between diodes

• Corresponds to well-center separation

Well Plate Holder

6mm belt• Commonly used in 3-D printers

20 T Pulley

OD/FI 384 Well Plate

Full LED Assembly

Well Plate

Selection Assembly

Outer Belt & Pulley• NEMA 17 at max 500 rpm

• 0.1 mm per step/pulse

Inner Belt & Pulley• Moves Well Plate Selection Assembly

inside the Full LED Assembly

5mm LEDs• 13 count

• 600 nm

OD/FI Non-384 Well Plate

8"

5"

5"

12 mm & 6 mm lenses• collimating

FI Diodes

Acrylic Filter Panel• Filters excitation emission from cultures

Beam Isolation Panel

Beam Redirection Holes• Correspond to number of wells in each

column of different well plate sizes

OD/FI Non-384 Well Plate

Well Plate

Size

Number of

Columns

6 3

24 6

48 8

96 12

Motor Torque Nm Torque kg-cm

Inner 0.025926 0.26437

Outer 0.112338 1.14554

NEMA 17 3.7

Motor Torque Calculation (t2cnc.hu)

Why NEMA 17? How Long Does it Take?

At maximum speed 0.1 mm per step

Well Plate

Size

Time for Diode

Measurement

Total

Measurement Time

6 15 ms 0.39828 s

24 30 ms 0.41328 s

48 40 ms 0.42328 s

96 60 ms 0.44328 s

OD/FI Non-384 Well Plate

Conical TubesLEDs and diodes aligned with tube

centers

Plate Movement

Plate Movement Linear Motion Assembly• NEMA-17 stepper motor

• Drives screw

Circular Motion Assembly• NEMA-17 stepper motor

• Ball bearings

• Orbital motion

• 20 mm

Rails• Ensures only linear

motion

Climate Control - Casing

Climate Control - CasingInsulating Capabilities

• 1D, steady state conduction

• Q=3.54 W between two

containers

• Q=5.22 W between container

and environment

• Total Q=29.64 W per

container

𝑞 =𝑇1 − 𝑇2

1ℎ𝑔1𝐴

+𝐿𝑎1𝑘𝑎𝐴

+𝐿𝑠𝑘𝑠𝐴

+𝐿𝑎2𝑘𝑎𝐴

+1

ℎ𝑔2𝐴12”

Automatic Drawer• MPC 8” Linear Actuator

• Newark 40 N Linear Solenoid

• 2” Compression Spring,

k=5.3 in/lb

Climate Control – Temperature Control

Climate Control - Temperature

•Total Wattage Required:

60 W

•Q = 4441.48 W

generated from device

•Delta T = 2.36 minutes

from heat sink (between

max and min)6.2 in

2 in

Heating/Cooling Device

Heat Sink

Climate Control – Gas

Climate Control - Gas

• Evacuation by purging requires 1.5*V

• Plugging in V, it requires roughly 43.52 L

• Gas from wall outlets existing in the lab

• Spring-controlled grip• F = -kx

• Force required : 87.23N

• Spring constant: 18.92 N/mm

• Stepper motor for rotation

• Cap added to well plate lid

Cap and Lid Removal

9.43 in

No cross contamination No cross contamination between individual wells/tubesNeed 40

Liquid Dispensing

Liquid Dispensing

Fluid Handling Capabilities

• 5000 μL pipette tips• Exit diameter: 5.4 mm

• Stepper motor:• Micro step of 1.25 μm

• Volume accuracy:

• 𝑉𝑐𝑦𝑙𝑖𝑛𝑑𝑒𝑟 = 𝜋𝑟2ℎ

= 𝜋 5.4𝑚𝑚 2 1.25𝜇𝑚

= 1.145𝑥108𝜇𝑚 = 0.115𝜇𝐿

• Dispensing precision of ±0.1 𝜇𝐿

Motor

Housing

Tip Ejector

Mechanism

15 mm

Disposable

Pipette Tip

Lead Screw

and Plunger

NEMA-17

Overall

View

Material Transport

Spring Tension Mechanism

• Keeps gears in contact with rack

• Accommodates deflection

Material Transport

Lead Screw Z-Axis Motor

• NEMA-17 Motor

• Mounted on ball bearing

linear carriage

Rack and Pinion Gantry

• X-Y Motion

• 3:1 Gear Belt Reduction

• 0.0006” per step

Proximity Sensor

User Interface

User Interface

•P = IV = 6(120) = 720 W

(Emergency stop)

•Design's total power

usage: 407.47 W

•3 LED indicators

•Raspberry Pi with

smoothie board for

control

•DAQ for sensors and

storing data

Button Housing: 6" x 5" x 4.5"

Tablet: 13.5" x 11.25"

4.5”

6”

5”

Structure/Enclosure

Structure/Enclosure

BSL-2 Biosafety Cabinet• 100% air exhaust with HEPA

Filter

• 105 CFM DC Blower

• 100 LFPM

through 32"x4.75" inlet

Acrylic Glass Case• 0.125” thick sheet• Thermal Conductivity:

0.170 W

𝑚𝐾

• Rubber coated door handle

Aluminum Frame•1” x 1” x 0.125” square tubing for frame

•1.5” x 1.5” square bar for supporting rails

Cost EstimateMaterial/Process Cost Estimate

OTS Parts $4658.77

Modified OTS Parts $789.32

Raw Materials $1152.32

Manufacturing Labor $2579.32

Assembly Labor $144.81

Energy Consumption $123.26

Total: $9447.80

Cultures multiple

microbes simultaneously

Individual cell environments

Minimal human

interaction

Why Choose MECCR?

Thank you for your time!