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MPI MODULAR SYSTEMS
Announcing 5 New Precision Plug-In Units
MP-1004A Log Ratio Amplifier. 6 decades range: 10 3 to 10 ' A. 100 mv/decade output. Built-in reference gives log of single input. Accepts positive or negative inputs.
MP-1030A High Voltage Power Supply. -500 to - 1500 V. Adjustable current limit protects PM tubes. 3 operating modes: fixed voltage, programmable voltage and constant PM current.
MP-1039 Dual, FET Operational Amplifiers. Input current typically 3 pA at either input. 1012 ohms resistance at either input. Open loop gain 2x104 minimum. 6V//isec slew rate.
MP-1040 Multiplier. Seven switch selected functions: A710; AxB/10; 10A/B, B > 0 ; 10A/B, B < 0 ; V - B , B < 0 ; VB, B > 0 . Four quadrant multiplier.
MP-1041 Instrumentation Amplifier. Provides gains from 1 to 1000. Range switch and 10-turn potentiometer. Input impedance, differential and common mode, 10,! ohms typ. Guarded inputs.
MPI McKEE-PEDERSEN INSTRUMENTS
Phone 415-937-3630 Box 322, Danville, CA 94526 USA
CIRCLE 155 O N READER SERVICE CARD
na tura l ly occurring proteins , usual ly n o n i m m u n e globulins, which have specific affinity for the hormone or a class of hormones . E x a m p l e s include m a n y steroid hormones , thyroxine, v i t amin D3 and its metabol i tes , and v i t amin B12. The rad ioenzymat ic assay employs an enzyme as t he reactive agent , and the separa t ion of two radioact ive pools involves separa t ion of two compounds , one of which was formed from the other by the enzy-mat ical ly cata lyzed react ion. An exa m p l e is t he folic acid assay by use of folic acid reductase . T h e radioreceptor assay employs a par t ia l ly purified t issue receptor as the react ive agent . Examples include adrenocort icotropin (ACTH) , cyclic A M P , and cyclic G M P . T h e immunorad iomet r i c assay differs from the RIA and other re la ted methods . It uses a radioact ively labeled purified an t ibody . Unreac ted and reac ted labeled ant ibodies are separa ted by adsorpt ion to ant igen which is bound to a solid suppor t . Examples include insulin, growth hormone, calcitonin, and para thyro id hormone .
Generally, the RIA has numerous advan tages over related methods . Compared to C P B , RIA has greater sensit ivity and specificity, so t h a t the specimens do no t require chromatography or o ther purif ication s t eps . T h e radioreceptor assay is res t r ic ted to compounds for which t issue receptors can be identified and isolated, although they do have one advan tage of measur ing "biologic ac t iv i ty" as opposed to " immunologic ac t iv i ty ." However, if t he RIA is properly valida ted , there is no essential difference. T h e rad ioenzymat ic assay m a y be able to measure compounds for which ant ibodies cannot be readily m a d e bu t has the d i sadvan tage of requiring separa t ion of compounds of s imilar size a n d s t ruc ture for quan t i t a t i on . T h e immunorad iomet r i c assay does not have some of t he " b l a n k " problems of the RIA b u t is costly in its use of an t i se rum and ant igen and is more difficult to set u p initially.
Bibliography The first section includes a number of
reviews, symposia, and workshops on radioimmunoassay and related methods. The additional sections include references on specific subjects, although most of the references in the General section also cover these topics.
General 1. S. A. Berson, R. S. Yalow, S. M. Glick,
and J. Roth, Immunoassay of Protein and Peptide Hormones, Metabolism, 13, 1135(1964).
2. S. A. Berson and R. S. Yalow, Immunoassay of Protein Hormones, in "The Hormones," G. Pincus, K. V. Thimann, and E. B. Astwood, Eds., Vol 4, pp 557-630, Academic Press, New York, N.Y., 1964.
3. J. T. Potts, Jr., L. M. Sherwood, J. L.
H. O'Riordan, and G. D. Aurbach, Radioimmunoassay of Polypeptide Hormones, Advan. Int. Med., 13, 183 (1967).
4. S. A. Berson and R. S. Yalow, General Principles of Radioimmunoassay, Clin. Chim.Acta, 22,51 (1968).
5. E. Diczfalusy, Ed., "Immunoassay of Gonadotrophins," Acta Endocrinol., 63, Supplementum 142 (1969).
6. M. Margoulies, Ed., "Protein and Polypeptide Hormones," Part 1, Excerpta Med. Int. Congr. Series No. 161, Amsterdam, the Netherlands, 1969.
7. Β. Ε. P. Murphy, Protein Binding and the Assay of Non-Antigenic Hormones, Rec. Progr. Harm. Res., 25, 563 (1969).
8. E. Diczfalusy, Ed., "Steroid Assay by Protein Binding," Acta Endocrinol., 64, Supplementum 147 (1970).
9. G. H. Grant and W. R. Butt, Immunochemical Methods in Clinical Chemis-trv, Advan. Clin. Chem., 13,383(1970).
10. J. W. McArthur and T. Colton, Eds., "Statistics in Endocrinology," MIT Press, Cambridge, Mass., 1970.
11. F. G. Peron and B. V. Caldwell, Eds., "Immunologic Methods in Steroid Determination," Appleton-Century-Crofts, New York, N.Y., 1970.
12. W. D. Odell and W. H. Daughaday, Eds., "Principles of Competitive Protein-Binding Assays," Lippincott, Philadelphia, Pa., 1971.
13. K. E. Kirkham and W. M. Hunter, Eds., "Radioimmunoassay Methods," Churchill Livingstone, Edinburgh, Scotland, 1971.
14. A. R. Midgley, Jr., G. D. Niswender, V. L. Gay, and L. E. Reichert, Jr., Use of Antibodies for Characterization of Gonadotropins and Steroids, Rec. Progr. Horm. Res., 27.235(1971).
15. D. S. Skelley, L. P. Brown, and P. K. Besch, Radioimmunoassay, Clin. Chem.. 19,146(1973).
Principle and Treatment of Data 16. G. Scatchard, The Attraction of Pro
teins for Small Molecules and Ions, Ann. N.Y. Acad. Sci., 51, 660 (1949).
17. D. Rodbard, P. L. Rayford, J. A. Cooper, and G. T. Ross, Statistical Quality Control of Radioimmunoassays, J. Clin. Endocrinol. Metab., 28, 1412 (1968).
18. R. Ekins and B. Newman, Theoretical Aspects of Saturation Analysis, Acta Endocrinol., 64, Supplementum 147, 11 (1970).
19. R. S. Yalow and S. A. Berson, Radioimmunoassays, in "Statistics in Endocrinology," J. W. McArthur and T. Colton, Eds., pp 327-44, MIT Press, Cambridge, Mass., 1970.
20. W. G. Duddleson, A. R. Midgley, Jr., and G. D. Niswender, Computer Program Sequence for Analysis and Summary of Radioimmunoassay Data, Corn-put. Biomed. Res., 5, 205 (1972).
Antiserum Preparat ion and Evaluation 21. J. Freund, The Effect of Paraffin Oil
and Mycobacteria on Antibody Formation and Sensitization, Amer. J. Clin. Path., 21,645(1951).
22. G. D. Niswender and A. R. Midgley, Jr., Hapten Radioimmunoassay for Steroid Hormones, in "Immunologic Methods in Steroid Determination," F. G. Peron and B. V. Caldwell, Eds., pp 149-73, Appleton-Century-Crofts, New York, N.Y., 1970.
23. B. A. L. Hum and J. Landon, Antisera for Radioimmunoassay, in "Radioimmunoassay Methods," Κ. Ε. Kirkham and W. M. Hunter, Eds., pp 121-42, Churchill Livingstone, Edinburgh, Scotland, 1971.
24. W. D. Odell, G. A. Abraham, W. R. Skowsky, M. A. Hescox, and D. A. Fish-
888 A · ANALYTICAL CHEMISTRY, VOL. 45, NO. 11, SEPTEMBER 1973