M I C H A E L B E R M A N

41 E S S E X S T R E E T #2 , C A M B R I D G E , M A S S A C H U S E T T S 02139(617) 642-4136 | WONGBERMAN@HOTMAIL.COM

S U M M A R YResourceful research scientist and Lab Manager with experience in a range of techniques and lab organization. Responsible Laboratory Safety Officer and departmental representative. Meticulous detail and concept oriented experimental scientist. Independently plans and executes experiments, and analyzes data. Maintains lab records and biological stocks. Purchases supplies via online marketplace. Enjoys training personnel, and effective working in teams with collaborators. Knowledge of animal work in a barrier facility. Enthusiastic both about getting the job done, and gaining new insights into the science.

T E C H N I C A L S K I L L SPCR Techniques:• RT – qPCR • Nested and inverse PCR• Site Directed Mutagenesis Methods • PCR fusion• Design and use of Degenerate primers & probes • Colony PCR

Cloning Techniques• Ligation • Gibson assembly • Gateway• Inverse KLD PCR • PCR fusion mutation • Blunt End Polishing• Construction and screening of bacteriophage and plasmid libraries

• RNA/Genomic Primary Gene Cloning

Purifications• DNA • RNA • Protein• Silica Column • Anion Exchange • Electrophoresis• FPLC • Precipitations • Extractions

Tissue and Bacterial Culture:• Cell line maintenance • Transfection • RNAi• Cell Stimulation Assays • Titers • Bacterial Competence• Preparing solutions and media • Plate assays • Hybridoma Cloning

Detection Methods and Assays:• Western Blot • Luciferase Assay • RNAse Protection Assay• Northern Blot • qPCR • Luciferase Assay• Southern Blot • PCR Genotyping • RPA, SSCP, RIA, ELISA, etc.

Bioinformatics & Sequencing• Sequence alignments • Sequence Searches • DNAstar, & GCG sequence analysis• Sanger DNA sequencing using M13 bacteriophage vectors

E X P E R I E N C EHARVARD MEDICAL SCHOOL, MBIB Dept., Boston, MAResearch Assistant II, Lab Manager, Lab Safety Officer 1987 – current

• Maintained Laboratory supplies and biological stocks.

• Supervised laboratory safety, conducted radiation surveys, maintained chemical waste satellite area, and trained personnel.• Cloned hundreds of genes into expression vectors in support of laboratory projects, derived from existing clones, as well as primary RNA and genomic DNA templates. • Characterized genes by Southern, Northern, and Western blots.• Analyzed gene expression by PCR, qPCR, RPA, luciferase reporter assays.• Cloned and sequenced novel genes, including CCL1, and mouse CXCR4, the HIV coreceptor.• Designed and utilized genotyping assays for mouse breeding program, including optimized duplex SYBR Green primer sets.• Performed luciferase assays for promoter studies or as a signaling readout.• Analyzed proteins by Western Blot and Immunoprecipitation to verify new clones, and to characterize genes’ responses and interactions.• Tissue culture cell line maintenance, as well as cell stimulation, for analysis by RT-PCR, Western, or Northern blot.• Cloned novel genes using degenerate and nested PCR techniques, in conjunction with library screening or PCR methods.

MASSACHUSETTS GENERAL HOSPITAL, Boston, MAResearch technician, 10/1986 –1987• Cloned hybridomas, maintained by tissue culture, and performed assay screens for identification of the somatostatin receptor.

DANA FARBER CANCER INSTITUTE, ImmunoPathology Dept, Boston, MAResearch technician, 9/1984 – 8/1986• Screened negative selection library to discover novel genes.• Assisted in research on the mouse T-cell receptor gene and cloned novel T-cell genes. • Constructed and screened cDNA libraries using bacteriophage and plasmid vectors. Characterized resultant clones by restriction mapping, Sanger sequencing, Southern, and Northern blot analysis.• Maintained supplies, and prepared solutions.

Rosenstiel Center, Brandeis University, Waltham, MALaboratory technician, 8/1982 – 8/1983• Assisted in a study of Bacillus subtilis and Bacillus licheniformis sporulation genes. • Performed molecular biology experiments and maintained the laboratory.• Prepared solutions and media.

Berman’s Motor Express, Medford, MA.• Clerk and dispatch assistant. 2/1981 – 6/1982• Maintenance of records, customer relations, and expedition of shipments.

Interactive Data Corporation, Waltham, MA• Data transmissions operator, 6/1979 – 8/1979• Responsible for processing of computer share draft data and transmission to client organizations.

E D U C A T I O NBrandeis UniversityB.A. Biology

R E F E R E N C E SAvailable upon request.

I N T E R E S T S

Cooking, Baking whole grain breads, Running, Biking, Yoga, Gardening, Health, Politics, and the Environment.

P U B L I C A T I O N S

TRIM65 regulates microRNA activity by ubiquitination of TNRC6.Li S, Wang L, Fu B, Berman MA, Diallo A, Dorf ME.Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5.

The ubiquitin conjugating enzyme UBE2L3 regulates TNFα-induced linear ubiquitination.Fu B, Li S, Wang L, Berman MA, Dorf ME.Cell Res. 2014 Mar;24(3):376-9.

Mapping a dynamic innate immunity protein interaction network regulating type I interferon production.Li S, Wang L, Berman M, Kong YY, Dorf ME.Immunity. 2011 Sep 23;35(3):426-40. doi: 10.1016/j.immuni.2011.06.014. Epub 2011 Sep 8. Erratum in: Immunity. 2011 Oct 28;35(4):647-8.

Mapping a dynamic innate immunity protein interaction network regulating type I interferon production.Li S, Wang L, Berman M, Kong YY, Dorf ME.Immunity. 2011 Sep 23;35(3):426-40. doi: 10.1016/j.immuni.2011.06.014. Epub 2011 Sep 8. Erratum in: Immunity. 2011 Oct 28;35(4):647-8.

RNAi screen in mouse astrocytes identifies phosphatases that regulate NF-kappaB signaling.Li S, Wang L, Berman MA, Zhang Y, Dorf ME.Mol Cell. 2006 Nov 17;24(4):497-509.

T-cell properties determine disease site, clinical presentation, and cellular pathology of experimental autoimmune encephalomyelitis.Abromson-Leeman S, Bronson R, Luo Y, Berman M, Leeman R, Leeman J, Dorf M.Am J Pathol. 2004 Nov;165(5):1519-33.

Low nuclear levels of nuclear factor-kappa B are essential for KC self-induction in astrocytes: requirements for shuttling and phosphorylation.Zhai Q, Luo Y, Zhang Y, Berman MA, Dorf ME.Glia. 2004 Dec;48(4):327-36.

Tumor necrosis factor is required for RANTES-induced astrocyte monocyte chemoattractant protein-1 production.Luo Y, Berman MA, Abromson-Leeman SR, Dorf ME.Glia. 2003 Aug;43(2):119-27.

RANTES stimulates inflammatory cascades and receptor modulation in murine astrocytes.Luo Y, Berman MA, Zhai Q, Fischer FR, Abromson-Leeman SR, Zhang Y, Kuziel WA, Gerard C, Dorf ME.Glia. 2002 Jul;39(1):19-30.

Low nuclear levels of nuclear factor-kappa B are essential for KC self-induction in astrocytes: requirements for shuttling and phosphorylation.Zhai Q, Luo Y, Zhang Y, Berman MA, Dorf ME.Glia. 2004 Dec;48(4):327-36.

Astrocytes express functional chemokine receptors.Dorf ME, Berman MA, Tanabe S, Heesen M, Luo Y.J Neuroimmunol. 2000 Nov 1;111(1-2):109-21.

Modulation of experimental autoimmune encephalomyelitis: effect of altered peptide ligand on chemokine and chemokine receptor expression.Fischer FR1, Santambrogio L, Luo Y, Berman MA, Hancock WW, Dorf ME.J Neuroimmunol. 2000 Oct 2;110(1-2):195-208.

The orphan seven-transmembrane receptor apj supports the entry of primary T-cell-line-tropic and dualtropic human immunodeficiency virus type 1.Choe H1, Farzan M, Konkel M, Martin K, Sun Y, Marcon L, Cayabyab M, Berman M, Dorf ME, Gerard N, Gerard C, Sodroski J.J Virol. 1998 Jul;72(7):6113-8.

Cloning and chromosomal mapping of an orphan chemokine receptor: mouse RDC1.Heesen M, Berman MA, Charest A, Housman D, Gerard C, Dorf ME.Immunogenetics. 1998 Apr;47(5):364-70.

Identification of a new mouse beta-chemokine, thymus-derived chemotactic agent 4, with activity on T lymphocytes and mesangial cells.Tanabe S, Lu Z, Luo Y, Quackenbush EJ, Berman MA, Collins-Racie LA, Mi S, Reilly C, Lo D, Jacobs KA, Dorf ME.J Immunol. 1997 Dec 1;159(11):5671-9.

Murine astrocytes express a functional chemokine receptor.Tanabe S, Heesen M, Berman MA, Fischer MB, Yoshizawa I, Luo Y, Dorf ME.J Neurosci. 1997 Sep 1;17(17):6522-8.

Functional expression of the CXC-chemokine receptor-4/fusin on mouse microglial cells and astrocytes.Tanabe S, Heesen M, Yoshizawa I, Berman MA, Luo Y, Bleul CC, Springer TA, Okuda K, Gerard N, Dorf ME.J Immunol. 1997 Jul 15;159(2):905-11.

Alternate splicing of mouse fusin/CXC chemokine receptor-4: stromal cell-derived factor-1alpha is a ligand for both CXC chemokine receptor-4 isoforms.Heesen M, Berman MA, Höpken UE, Gerard NP, Dorf ME.J Immunol. 1997 Apr 15;158(8):3561-4.

Cloning of the mouse fusin gene, homologue to a human HIV-1 co-factor.Heesen M, Berman MA, Benson JD, Gerard C, Dorf ME.J Immunol. 1996 Dec 15;157(12):5455-60.

Mouse astrocytes respond to the chemokines MCP-1 and KC, but reverse transcriptase-polymerase chain reaction does not detect mRNA for the KC or new MCP-1 receptor.Heesen M, Tanabe S, Berman MA, Yoshizawa I, Luo Y, Kim RJ, Post TW, Gerard C, Dorf ME.J Neurosci Res. 1996 Aug 15;45(4):382-91.

Cloning and characterization of a novel T cell activation gene.Burd PR, Freeman GJ, Wilson SD, Berman M, DeKruyff R, Billings PR, Dorf ME.J Immunol. 1987 Nov 1;139(9):3126-31.