MB-PO13T

S. graminisoli NBRC 108883T S. shenzhenensis DSM 42034T

Fig. S1. Substrate mycelium appearance of strain MB-PO13T and related Streptomyces species on ISP 2 agar plate after incubation at 28 °C for 7 days.

S. gramineus NBRC 108885T S. rhizophilus NBRC 107863T

min0 10 20 30

mAU

0

50

100

150

200

250

300

nm300 400 500 600 700

mAU

050

100150200250

hyaluromycin

MB-PO13T

S. shenzhenensis DSM 40343T

S. graminisoli NBRC 108883T

O O

O

O

OH

OH

MeO

OHOO

HN

O

O

HO

Column: COSMOSIL 5C18-AR-II (4.6 × 250 mm)Solvent: MeCN / 0.1% HCO2HFlow: 1.0 mL/minDetector: 254 nm

Fig. S2. HPLC chromatogram of BuOH extracts of strain MB-PO13T and related Streptomyces species cultured in yeast extract-starch liquid medium at 28 °C for 7 days. MeCN concentration was 15%–85% for 0–30 min.

Fig. S3. Maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences of strain MB-PO13T and its taxonomic neighbours. Kitasatospora setae KM-6054T

(AB022868) was used as the outgroup. Bootstrap values (>70%) based on 1,000 replicates are shown at branch nodes.

S. tricolor LMG 20328T (AJ781380) S. bangladeshensis AAB-4T (AY750056) S. chiangmaiensis TA4-1T (AB562507)

S. mutabilis C.B.239T (KF991631) S. rochei SM3T (JN128892) S. minutiscleroticus NRRL B-12202T (EF178696)

S. hyaluromycini MB-PO13T (AB184533) S. graminisoli JR-12T (HQ267994)

S. shenzhenensis 172115T (HQ660226) S. jiujiangensis JXJ 0074T (KF938657)

S. niveoruber MA1T (JF827351) S. murinus MDMT-5T (KF973308)

S. rhizophilus JR-41T (HQ267989) S. gramineus JR-43T (NR 109017)

S. katrae NRRL B-3093T (EF654092) S. lavendulae IFO 3125T (D85106)

S. galbus DSM 40089T (NR 026178) S. capoamus JCM 4734T (NR 040856)

S. hygroscopicus subsp. limoneus ATCC 21431T (X79853) S. hawaiiensis NRRL 15010T (EU624140)

S. tumescens OTP-3-1T (AF346484) Kitasatospora. setae KM-6054T (AB022868)

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85

91

72

0.01

Fig. S4. Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences of strain MB-PO13T and its taxonomic neighbours. Kitasatospora setae KM-6054T

(AB022868) was used as the outgroup. Bootstrap values (>70%) based on 1,000 replicates are shown at branch nodes.

S. jiujiangensis JXJ 0074T (KF938657) S. niveoruber MA1T (JF827351) S. murinus MDMT-5T (KF973308) S. shenzhenensis 172115T (HQ660226) S. hyaluromycini MB-PO13T (AB184533) S. graminisoli JR-12T (HQ267994)

S. tricolor LMG 20328T (AJ781380) S. bangladeshensis AAB-4T (AY750056)

S. chiangmaiensis TA4-1T (AB562507) S. mutabilis C.B.239T (KF991631) S. rochei SM3T (JN128892) S. minutiscleroticus NRRL B-12202T (EF178696) S. rhizophilus JR-41T (HQ267989) S. gramineus JR-43T (NR 109017) S. katrae NRRL B-3093T (EF654092) S. lavendulae IFO 3125T (D85106) S. capoamus JCM 4734T (NR 040856) S. galbus DSM 40089T (NR 026178) S. hygroscopicus subsp. limoneus ATCC 21431T (X79853) S. hawaiiensis NRRL 15010T (EU624140) S. tumescens OTP-3-1T (AF346484) Kitasatospora setae KM-6054T (AB022868)

100

100

95

88

99

81

DPG

PI

PE

PL6

PL5

PL1

PL2

PL4

PL3

Fig. S5. Two-dimensional thin-layer chromatogram of polar lipids from strain MB-PO13T Abbreviations: DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PL1–PL6, unidentified polar lipids. The spray reagent, 5% molybdophosphoric acid.

1st

2nd

Table S1. Cellular fatty acid composition (%) of strain MB-PO13T and related Streptomyces species.Strains: 1, strain MB-PO13T; 2, S. graminisoli NBRC 18883T; 3, S. shenzhenensis DSM 42034T; 4, S. rhizophilus NBRC 108885T; 5, S. gramineus NBRC 107863T; 6, S. jiujiangensis JXJ 0074T. The analyses of 1 to 5 were performed in parallel in this study. Data of 6 are from Zhang et al.31 Bold type shows the major components (>10%). –, Not detected; tr, traces (<1%); ND, no data.

Fatty acid 1 2 3 4 5 6

Saturated fatty acidsStraight-chain

C12:0 – – – – tr NDC13:0 – – tr tr tr NDC14:0 tr 1.4 tr tr 1.5 NDC15:0 2.6 4.3 5.2 – – NDC16:0 10.7 8.7 6.1 6.5 9.2 NDC17:0 tr tr 1.0 tr tr ND

Branched iso-C12:0 tr tr – tr tr NDiso-C13:0 tr tr tr tr tr NDiso-C14:0 15.0 9.1 8.2 10.8 10.3 2.6iso-C15:0 8.9 8.6 11.4 9.7 13.5 4.3iso-C16:0 23.4 24.1 21.6 32.6 21.2 17.7iso-C17:0 2.4 1.3 2.6 1.8 2.2 NDanteiso-C13:0 tr tr tr tr tr NDanteiso-C15:0 24.9 25.7 27.7 25.7 31.6 43.6anteiso-C17:0 5.6 5.7 6.6 4.3 4.8 15.8

Cycliccyclohexyl-C17:0 1.1 tr tr tr tr ND

Mono-unsaturated fatty acids

C15:1 B – tr – – – NDiso-C16:1 H tr 1.5 1.3 1.5 tr 3.8C16:1 ω9c 1.1 3.4 1.8 – NDC17:1 ω8c – – – tr tr NDC17:1 ω9c – tr tr – – NDiso-C17:1 ω9c – – – 1.0 tr NDanteiso-C17:1 C tr 1.2 1.6 – – NDanteiso-C17:1 ω9c – – – tr tr 6.7

9-Methyl fatty acid9-Methyl C16:0 tr tr 1.7 – – ND