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©Copyright 2014 May I Help You 1
May I Help You? Infection Control Training for Unregistered Dental Assistants
Course Description This 8 hour course focuses on all aspects of infection control in a dental healthcare setting including
disinfection and sterilization, universal & standard precautions, and operatory set-up and clean-up. In
this course, students will learn and practically apply the dental assistant’s responsibilities in the office
according to the updated Center for Disease Control (CDC) guidelines and regulations from the Dental
Board of California. Upon the successful completion of this course, students will meet the requirements
of sections 1750 or 1752:1 from the Dental Board of California.
This is a two-part course. The lecture material is provided online for self-paced study. Part II is an in-
person session that begins with a test covering the online material, followed by four-hours of clinical
application and assessment.
Learning Objectives (LO) Upon completion of this course, students will be able to:
1. Explain the importance of implementing minimum standards for infection control in the
Dental Healthcare Setting (DHS);
2. Describe the role of CDC & OSHA in infection control;
3. Understand Dental healthcare personnel’s (DHCP) responsibility to comply with and
incorporate the minimum standards in their daily work practices;
4. Describe potential effects of not incorporating minimum standards for infection control to
the DHS;
5. Define the causes of infection and the different types of microorganisms that contribute to
infection in the dental healthcare environment;
6. Explain the chain of infection and modes of transmission;
7. Summarize strategies and practices for prevention and control of pathogens;
8. Describe the post-management procedure and follow-up of an exposure injuries;
9. Explain the different types of waste generated in a dental office, and the correct way to
handle, store, and dispose of the various types of waste using infection control guidelines;
10. Describe and demonstrate proper hand hygiene procedures for DHCP;
11. Demonstrate correct techniques for wearing and removing personal protective equipment
(PPE) in order to effectively protect against splatter, splash and contamination;
12. Demonstrate proper disinfecting techniques for clinical contact surfaces;
13. Perform disinfection and sterilization of patient care items according to infection control
guidelines;
14. Perform specific Dental Boards requirement to maintain safe dental unit water lines.
15. Demonstrate correct infection control disinfection and protection strategies when
performing lab procedures.
©Copyright 2014 May I Help You 2
DETAILED COURSE OUTLINE
Section 1: Introduction/Overview (Online) A. Potential Effects of Infection to the Dental Healthcare Setting (DHS)
1. Due to the nature of their work, Dental Healthcare Personnel (DHCP) come in contact
with a variety of potentially infectious diseases on a daily basis and risk both contracting
and transmitting these diseases.
2. In order to prevent and reduce healthcare associated infections, it is critical that DHCP
comply with mandated regulations, recommended guidelines, practices, and
precautions, thereby reducing the risk of transmission of infectious disease from patient
to patient.
B. Recommending and Regulatory Agencies
1. The CDC is part of the Public Health Service, which is a division of the US Department of
Health. It is not a regulatory agency; however, it issues health and safety
recommendations based on scientific research.
a. The CDC is concerned with minimizing the transmission and spread of infectious
diseases in the DHS.
b. The CDC issued the first recommendations for DHS to prevent the transmission
of blood-borne disease in 1986. In 2003, the CDC issued its Guidelines for
Infection Control in Dental Healthcare Settings, which expands on its earlier
guidelines and added new information.
c. In 2003, the CDC created “Guidelines for Infection Control in DHS in order to
educate DHCP of strategies and precautions to minimize spread of infection.
2. OSHA is a regulatory agency, meaning that it is mandatory for DHCP to implement,
practice, and adhere to all OSHA guidelines and policies. Concerned with safety and
health of DHCP (employees)
a. OSHA is concerned with the safety and health of an organization’s employees
(DHCP).
b. OSHA created the Bloodborne Pathogen Standard (BBP), which mandates
that specific regulations including Universal Precautions be followed in the
DHS, based on CDC’s recommended guidelines first published in 1986.
c. On December 1, 2013, OSHA adopted a new Hazard Communication Standard
known as the Global Harmonized System for Hazard Communication.
Employers are required to provide training to their employees regarding new
labeling, warnings, pictograms and the new format for Safety Data Sheets.
1. California Occupational Safety & Health Administration (CAL/OSHA) is a regulating
agency
a. In 2009, CAL/OSHA adopted the Aerosol Transmissible Disease (ATD) Standard
b. Any DHS in which patients with suspected or confirmed ATD (e.g. TB) must
comply with the ATD Standard
2. Dental Board of California (DBC) is a regulatory agency
c. The DBC created the “Minimum Standards for Infection Control” that must be
posted in every DHS
d. Every DHCP whether licensed or not must follow the DBC’s minimum
standards. And it is a requirement to renew a dental license.
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C. Consequences of Non-compliance
a. Non-compliance with the DBC’s Minimum Standards for Infection Control, OSHA’s
Bloodborne Pathogen Standard, and Global Harmonized System for Hazard
Communication, or CAL/OSHA’s Aerosol Transmissible Disease Standard could lead to
cross-contamination, infections, and illness to the dental patient and/or the DHCP.
b. Employees could contaminate their vehicles, or even their homes, if Universal
Precautions are not followed. Employee’s families could potentially suffer illnesses and
infections if OSHA regulations are not followed in the DHS.
c. Dentists could be subject to fines and/or have their offices closed by the Regulating
Agencies.
After completing Section 1, students will be able to explain the importance of implementing minimum
standards for infection control in the DHS (LO1); describe the roles of the CDC and OSHA in infection
control (LO2); understand their personal responsibility to comply with the standards and guidelines for
infection control (LO3); and describe potential effects of not incorporating the minimum standards for
infection control to the DHS (LO4).
Section 2: The Five Types of Microorganisms (Online) A. Bacteria: One-celled organisms that vary in size, shape, and arrangement
1. Human beings host a variety of beneficial bacteria that combat pathogens
2. Infection occurs when a specific type of bacteria invades an area of the body in which it
does not naturally occur
3. Bacterial diseases include: TB, Legionnaires’ disease, Tetanus, Syphillis, Methicillin-
Resistant Staphylococcus aureus (MRSA)
B. Algae: Can be microscopic, single-celled organisms, as well as large, multi-celled organisms
that contain chlorophyll. Found in freshwater and marine habitats and generally do not
produce disease
C. Protozoa: A large groups of single- or multi-celled organisms without a rigid cell wall.
1. Larger than bacteria, protozoa are found in freshwater, marine habitats, and soil.
2. Though in general protozoa do not cause disease, some are pathogenic and can lead to
intestinal infection in humans including: Malaria, diarrhea, and amebic dysentery
D. Fungi: Common single- or multi-celled organisms such as mushrooms, yeasts, and molds.
1. Certain types of mold and yeast can cause disease in humans affecting the lungs and
other organs, tissues, or the skin, nails, scalp, and hair.
2. Common fungal diseases includes pulmonary infections, candidiasis, and athlete’s foot
E. Viruses: Much smaller than bacteria, viruses have many different shapes, but all have a
nucleic acid core (DNA or RNA) surrounded by a capsid, or protein coating.
1. A virus lives and multiplies itself in a host cell.
2. First it invades a host cell, multiplies and replicates itself, and then enters the body by
destroying the host cell.
3. Types of Viral Diseases include Hepatitis A, B, and C; HIV, Herpes; Measles, the common
cold, influenza and mononucleosis
After completing Section 2, students will be able to define the causes of infection and the different types
of microorganisms that contribute to infection in the dental healthcare environment (LO5).
©Copyright 2014 May I Help You 4
Section 3: Disease Transmission (Online) A. Chain of Infection: A chain is an interconnected series of links. A chain of infection, then, is
the group of links that must be present in order for infection to occur. The following are the
links required for an infection to exist:
1. Pathogen (Virulence & Numbers): A pathogen is an infectious agent; an organism
capable of producing disease.
a. Virulence: The strength of an organism’s ability to produce disease.
b. Numbers: A high enough number of pathogenic microorganisms must be
present to produce disease.
2. Source or Reservoir: Reservoirs are where germs live and grow, and are the source of
pathogens. The primary source of pathogens in the Dental Healthcare environment is
the mouth of the patient: saliva, blood, and respiratory secretions.
3. Mode of Transmission: An infectious disease is capable of being transmitted and spread
to others. This is the link that we have control over since there are precautions that can
be taken against transmission (these precautions will be covered in section III). There
are several ways disease is transmitted:
a. Direct: Person-to-person contact; examples include droplets spread through
sneezing & coughing, contact with infected body fluids or lesions. Hepatitis,
HIV, and TB are examples of diseases spread through direct contact.
b. Indirect: Infected agents are first transmitted to an object, surface, or another
person, and then transferred to an individual. Examples include unwashed
hands, infected countertops, a dental chart handled by a gloveless DHCP.
c. Airborne (Droplet infection): Disease is spread through droplets of moisture in
the air that contain bacteria or viruses. Most contagious respiratory diseases
are transmitted through droplets in the air (generally caused by coughing
&/or sneezing).
d. Aerosol, Spray, or Spatter (Airborne): Terms used to describe droplets of
potentially infectious microorganisms.
i. Aerosols are the finest form of droplets and disease is generally spread
through inhaling infectious droplets. Aerosols are created by using high-
speed-handpieces and ultrasonic scalers.
ii. Sprays & Spatter are larger than aerosols and generally spread infection
through contact with non-intact skin (skin in which a cut, scrape, or
needle prick has occurred). They are created in the same ways as
aerosol, as well as when using the air-water syringe.
4. Portal of Entry: The means through which an infectious microorganism enters the body.
These are typically the mouth and nose for airborne pathogens, and non-intact skin for
bloodborne pathogens.
5. ‘Susceptible Host: Someone who is unable to resist infection by a specific infectious
agent. A compromised immune system can make one a susceptible host. Factors such as
fatigue, stress, poor eating habit, and cuts and scrapes in the skin can contribute to a
compromised immune system. It is important to note that at times even healthy
individuals are susceptible hosts for pathogens.
©Copyright 2014 May I Help You 5
B. Transmission in the Dental Office: As discussed in the previous section, there are many ways
in which patient microorganisms can be transmitted in a dental health care setting.
1. Patient to Dental Health Care Practitioner (DHCP): There are many ways microorganisms
can spread from the patient to the DHCP, which is why this pathway is the most difficult
to control. The modes of transmission (which were discussed in detail in the previous
section) are: Direct contact, droplet infection, and indirect contact.
2. DHCP to Patient: This pathway is rare; however, disease may be transmitted from DHCP
to patient if proper infection control procedures are not followed (these will be
discussed in detail in section IV). If a DHCP has lesions or other non-intact skin, of if a cut
or other injury occurs while the hands are in the patient’s mouth, it is possible to
transmit microorganisms.
3. Patient to Patient: Though extremely rare, patient-to-patient infection may occur when
contamination from instruments used from one patient is passed to another patient via
non-sterilized instruments, handpieces, surfaces, and hands.
4. Dental Office to Community: Spread of disease from the dental office to the community
can occur when contaminated items used on the patient are sent out, such as
impressions sent to a lab. DHCPs can transport microorganisms out of the office via their
hair or contaminated clothing.
5. Community to Patient: Patients can become infected via waterborne microorganisms
that colonize inside the dental unit after coming through the municipal water supply.
After completing Section 3, students will be able to explain the chain of infection and modes of
transmission (LO6).
Section 4: Prevention Strategies & Practices (Online) A. Overview of CDC Guidelines for Infection Control in the Dental Office
1. Use of Standard Precautions rather than Universal Precautions
2. Work restrictions for healthcare personnel infected with infectious disease
3. Post exposure management of occupational exposures to blood-borne pathogens
4. Selection of devices with sharps injury-prevention features
5. Hand hygiene products and surgical hand asepsis
6. Contact dermatitis and latex hypersensitivity
7. Sterilization of instruments
8. Laboratory infection control
9. Dental unit waterline concerns
10. Dental radiology infection control
11. Preprocedural mouth rinses for patients
12. Oral surgical procedures
13. Tuberculosis
14. Infection control program evaluation
B. Overview OSHA Bloodborne Pathogen Standard
1. Exposure Control Plan
a. Each dental office shall have a written exposure control plan that clearly
describes how the office complies with the BBP Standard.
©Copyright 2014 May I Help You 6
b. The Exposure Control Plan must be accessible to employees and updated at
least annually, or when there are significant changes to procedures or
processes.
2. Universal Precautions
a. OSHA’s created the term Universal Precautions. Universal Precautions is the
concept that all human blood and certain bodily fluids are treated as if they are
infected with blood-borne pathogens. The reason to practice Universal
Precautions is that it is impossible to know which patients may carry blood-
borne pathogens, so all dental healthcare personnel should treat every patient
as if they were infected. OSHA’s Universal Precautions primary concern is for
the Health and Safety of employees in the dental office.
b. Standard Precautions is term created by CDC and adopted by the DBC. It is
similar to Universal Precautions that extend the rationale to include all body
fluids, secretions, and excretions. CDC and DBC primary concern is the health
and safety of dental patients.
3. Exposure Determination
a. OSHA categorizes employees by the risks associated with their job functions.
i. Category I: Routinely exposed to blood, saliva, or both (Dentist,
assistant, hygienist, lab technician)
ii. Category II: May occasionally be exposed to blood, saliva, or both (front
office employee that may occasionally disinfect an operatory after
patient treatment, process contaminated instrument or x-rays and/or is
trained in CPR).
iii. Category III: Never exposed to blood, saliva, or both (Front office)
4. Post-exposure Management: Even with precautionary measures in place, exposure
Incidents do happen; therefore, the BBPS requires employers to have a written plan that
explains the steps and procedure to follow if an employee has an Exposure Incident (see
Section 5).
5. Hepatitis B Immunization: Employers must offer the HBV vaccination series to all
category I & II employees within the first ten day hire.
a. Right of Refusal
i. The employee, however, has the right to refuse the vaccination for any
reason. If the employee refuses the vaccine, s/he must sign a
Declination form.
ii. If the employee changes his/her mind at a later time, the employer
must provide the Hep. B vaccination series at no cost to the employee
b. The employer shall not make participation in a prescreening program a
prerequisite for receiving the Hep. B vaccination or for hiring the employee.
c. The vaccination process
i. Hepatitis B vaccine comes in a series of three separate injections. These
must be taken in the specified order.
ii. If the second or third injection of the series is missed, the employee
must start over.
©Copyright 2014 May I Help You 7
iii. In order to know if the employee is protected, a blood test for Hepatitis
B antibodies should be done one – two months after final vaccine
injection of the series.
iv. For the few who do not form antibodies, a second series of injections
can be taken to improve the response.
v. People who do not respond after the second series should be warned
that they may be susceptible to Hepatitis B virus.
vi.
6. Record Keeping
a. Employers are required to keep confidential employee medical records for all
employees for the length of employment, and for 30 years following end of
employment if an Exposure Incident happened during employment.
i. Medical records must include:
a) A file folder with the employees first and last name.
b) The employee’s social security number.
c) The signed Hep. B Declination form.
d) Current proof of immunity to Hep. B.
e) Dates of the vaccines
f) Records of exposure and post-exposure follow up
ii. The only person/s having access to this file is the employer and the
designated Safety Manager.
b. Employee Training Records: Employers must keep records documenting that
they have provided employees with OSHA required training.
i. Employers are required to provide employees with initial Bloodborne
Pathogen and Office Safety training, and then annual Bloodborne
Pathogen training.
ii. OSHA requires the required training be done during regular office hours
at no cost to the employee.
iii. Employers must keep documentation of all training sessions in the
office Injury and Illness Prevention Plan for at least three years.
After completing Section 4, students will be able to summarize strategies and practices for
prevention and control of pathogens (LO7).
Section 5: Exposure Injury Management & Procedures (Online) OSHA mandates that all dental offices must prepare a written exposer control plan that documents
the procedures that must be followed as a result of an exposure incident. An exposure incident is
when an employee comes in contact with a specific splash of blood, saliva or OPIM into the eyes,
nose, mouth, or non-intact, or is poked with a contaminated sharp.
A. Determination of employees covered under the standard: Any employee that has occupational
exposure (Categories I & II)
1. Any category I or category II employee is susceptible to exposure to blood, saliva, OPIM,
and pokes from contaminated sharps.
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2. Should an exposure incident occur, employer and employee must follow post-exposure
and follow-up procedures.
B. Exposure Control Plan
1. Hepatitis B Vaccination
2. Post-exposure procedures:
a) Following a report of an exposure incident, the employer shall make
immediately available to the exposed employee a confidential medical
evaluation and follow-up, including at least the following elements:
1) Send employee to Health Care Professional (HCP) with:
2) A copy of the BBP Standard.
3) The completed Incident report.
4) Employee's Hepatitis B vaccination status.
5) Employers Workers compensation Insurance information.
3. Post-exposure follow-up from health care professional (urgent care)
a) Evaluates Exposure Incident.
b) Arranges for testing of employee and source individual (if agreeable)
c) Notifies employee of results of all testing.
d) Provides post-exposure prophylaxis.
4. Record keeping
A. Within seven days of the exposure incident the HCP sends Written Opinion
(only) to employer:
B. Documentation that employee was informed of evaluation results and the need
for further follow-up.
C. Whether Hepatitis B vaccine is indicated and if vaccine was received.
D. Provides copy of HCP's Written Opinion to employee and a copy is kept in the
employees CONFIDENTIAL medical record.
E. Within 14 days of the exposure incident the employer must investigate the
exposure incident and complete a “SHARPS INJURY LOG” form.
After completing Section 5, students will be able to describe the post-management procedure and
follow-up of an exposure injury (LO8).
Section 6: Waste Generated in Dental Offices (Online) Federal, state, and local agencies regulate the various types of waste found in dental office. Often these
agencies require Dental Offices to have permits because they create waste that is biohazardous or
hazardous in nature. Specific guidelines and regulations address the manner in which the types of waste
are handled, stored and properly disposed. Waste found in dental offices is classification as follows:
A. General Waste: Waste that is created in the regular course of a day such as paper towels,
paper mixing pads, empty food containers and is nonhazardous and non-regulated.
1. Does not pose any hazard to humans.
2. Dispose of waste in a sturdy, covered trash receptacle.
B. Contaminated Waste: Waste that has been in contact with blood, saliva, and/or other bodily
fluid and includes patient napkins and used barriers.
©Copyright 2014 May I Help You 9
1. Generally does not pose a risk to humans and may be disposed of in the same manner
as general waste.
2. In cases in which the contaminated waste could potentially release blood, or other
potentially infected material (OPIM) such as in blood-saturate gauze pads, must be
disposed of and handled according to regulatory agency guidelines.
C. Hazardous Waste: Waste that contains toxicity, including photo processing chemicals,
extracted teeth with amalgam & cold sterile solutions.
1. Poses risk to humans and the environment.
2. Must be handled and disposed of according to regulatory agency guidelines.
D. Infectious/Regulated Waste (Biohazardous): Contaminated waste that has contains
pathogens strong and numerous enough to cause infectious disease.
1. Poses risk to humans
2. Must be handled and disposed of according to regulatory agency guidelines.
a. Medical Waste
1) Extracted Teeth, blood-soaked gauze & cotton rolls, human tissue from
surgical procedure must be treated according to the Medical Waste
Management Plan of California.
2) Contaminated Sharps (needles, scalpel blades, orthodontic wires)
i. Dispose of in a puncture-resistant, sealable, leak proof sharps
container that is properly labeled as Biohazard.
ii. Sharps containers have to be located where it is likely Sharps
will be generated (each operatory, sterilization area, and lab).
iii. Sharps safety is a serious matter; in order to reduce the risk of
exposure, safe handling must be practiced. Recapping needles
using the single-handed scoop technique, or an appropriate
recapping device must be used. Placing sharps in the Sharps
container as soon as possible reduces the risk of injury.
After completing Section 6, students will be able to explain the different types of waste generated in
a dental office, and the correct way to handle, store, and dispose of the various types of waste using
infection control guidelines (LO9).
Section 7: Hand Hygiene (Online & Clinical Application) In order to reduce the number of microbes present on the hands of DHCP, and lessen the number of
microbes transferred to surfaces they touch, all DHCP must follow the minimum standards for infection
control's guidelines for hand hygiene that include required times to wash hands, and specific methods
and materials to use.
A. Required Times to Wash Hands: DHCP must clean their hands according to the stated
methods and with the appropriate materials at the following times:
1. At the beginning if their work shift each day
2. Routine hand washing throughout the day
a. Before eating
b. After using the restroom
c. When hands are visibly soiled
©Copyright 2014 May I Help You 10
3. Before & after routine dental procedures
4. Before glove placement & after glove removal (including when re-gloving due to tear or
puncture)
5. After touching items potentially contaminated with blood or saliva
6. Before surgery
7. After contact with patient’s intact skin
8. After contact with non-intact skin, mucous membranes, body fluids
9. At the end of their work shift each day
B. Materials & Methods for Hand Washing: Depending on the type of patient treatment, DHCP
may use soap & water, antimicrobial hand wash, or alcohol-based hand rubs incorporating
the following methods:
1. Routine hand washing:
a. Remove jewelry, rings, etc.
b. Use paper towel, or other barrier, to turn faucet on and to apply the hand
cleaner (pump or hands free dispenser)
c. Scrub hands vigorously for 15-20 seconds
d. Wash from the finger tips to the wrist
e. Thoroughly rinse under a stream of water
f. Pat dry; never friction rub with disposable towels
g. Use disposable towel or other barrier to turn off faucet (Do Not touch faucet)
2. Antiseptic hand washing: If hands are not visibly soiled or contaminated DHCP may use
Antiseptic hand rub or hand sanitizer.
a. The Hand Sanitizer must contain 65% or more Ethanol Alcohol
b. Use a sufficient amount of alcohol- based antiseptic, hands should remain wet
for 30 seconds or until hands are dry. This method will not remove dirt.
c. Hand Sanitizer may be used 3 consecutive times before hands must be washed
with soap/water.
3. For surgical procedures:
a. Prior to a surgical procedure, a surgical scrub needs to be done with an
antimicrobial soap.
b. Wash with antimicrobial soap & water for 2 – 6 minutes. Scrub from the finger
tips and the forearm up to the elbow.
C. When to Refrain from Patient Care: According to the DBC’s Minimum Standards for
Infection Control, DHCP must refrain from providing direct patient care when:
1. The conditions of the hands may render DHCP or patients more susceptible to
opportunistic infection or exposure
2. Hands have any kind of oozing from a wound, cut, or dermatitis. In this case, DHCP shall
also refrain from handling patient care equipment.
3. When the above conditions are present, employee must be reassigned to a non-clinical
task until conditions improves.
D. Clinical Application & Competency
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After completing Section 7, students will be able to describe and demonstrate proper hand hygiene
procedures for DHCP (LO10).
Section 8: Personal Protective Equipment (PPE): (Clinical Application) As part of their daily responsibilities, Dental Assistants come in contact with potentially infectious
material. As discussed in previous sections, this can occur through patient blood, saliva, and aerosol s. In
order to protect from infection, all DHCP are required to wear items designed to protect them, and their
patients, from disease. These items must be provided at no cost to the employee by their employer and
laundered (as applicable) according to OSHA guidelines. . If PPE is damaged in anyway and rendered
ineffective, the employer is responsible to repair or replace as necessary.
The selection of PPE is determined by the procedures being performed by the DHCP. PPE includes but is
not limited to:
A. Gloves (latex or non-latex & heavy-duty utility): There are several different types of gloves
DHCP must use in the DHS. The specific type of glove used depends on the procedure being
performed. Gloves are considered medical devises and regulated by the U.S. Food and Drug
Administration (FDA).
1. Examination gloves are the most commonly used in the DHS and are made of latex or
vinyl
2. Sterile Surgical gloves are to be used during all oral surgeries and periodontal
treatments that involve cutting bone, significant amounts of blood, or saliva
3. Non-latex gloves – these gloves are made from nitrile, vinyl, and other non-latex
materials for use by DHCPs with latex sensitivity and/or allergies.
4. Overgloves are clear plastic gloves that may be used over treatment gloves to prevent
contamination of items handled during treatment
5. Utility gloves should be made of puncture resistant heavy latex or nitrile rubber and
worn when cleaning and disinfecting instruments and surfaces, and when handling
contaminated laundry.
B. Face Masks: DHCP must wear face masks in order to protect themselves from exposure to
infectious organisms spread through inhaling aerosol spray from handpieces, air-water
syringes, and/or splashes.
1. DHCP are required to wear masks when performing the following:
a. Patient treatment procedures
b. Disinfecting
c. Cleaning and sterilizing contaminated instruments.
2. Masks should:
a. Fit the face well
b. Be changed between patients or during treatment if they become visibly soiled
or moist
c. Never be worn below the nose or chin
d. Be disposed of after each patient
C. Eye Protection (Face Shields & Eyewear): In order to protect the eyes from exposure to
potentially infectious material, all DHCP must wear one of the approved forms of eye
protection.
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1. OSHA and the DBC’s Minimum Standards for Infection Control, eye protection
requirements:
a. Eye protection must have solid front and side protection
b. A chin length face shield must be worn during procedures when blood or other
bodily material may splash or splatter(masks must still be worn when wearing a
face shield)
c. Goggles and/or glasses with shields must be worn to protect DHCP from spatter
from handpieces and splashed chemical agents that could damage the eyes
d. Patients are required to wear special filtered-lens glasses during certain types of
laser treatments.
2. Please Note: Prescription Glasses DO NOT meet OSHA & DBC’s Minimum Standards for
Infection Control
D. Gowns: In order to protect the skin and underclothing from exposure to potentially
infectious material, all DHCP must wear protective gowns in the DHS. These gowns may be
disposable or reusable. Gowns must comply with, and be laundered according to, OSHA and
the DBC’s Minimum Standards for Infection Control.
1. Gowns worn in the DHS must be:
a. Made of fluid resistant material (no liquid can get through)
b. Have long sleeves and high necklines
c. Worn for disinfection, sterilization, and housekeeping procedures involving
germicides, or handling contaminated items
d. Worn when there is potential for aerosol spray, splashing, or spattering of
blood, OPIM, or chemicals
e. Changed daily or between patients when they become moist or visibly soiled
f. Removed when leaving treatment and lab areas
2. Handling & Laundering Reusable Gowns (& towels): Laundry (reusable gowns and
towels) that has been soiled with blood or other potentially infectious materials, or may
contain sharps must be handled according to Cal/OSHA BBP standards:
a. The employer shall ensure that employees who have contact with contaminated
laundry wear heavy duty nitrile gloves and other appropriate personal
protective equipment
b. Contaminated laundry may never be taken home either by an employee or the
employer
c. Contaminated laundry shall be bagged or containerized at the location where it
was used and shall not be sorted or rinsed in the location of use (e.g. the dental
office)
d. When contaminated laundry is wet, the laundry shall be placed in a container
that prevents soak-through and/or leakage of fluids to the exterior
E. Clinical Application & Competency
After completing Section 8, students will be able to demonstrate correct techniques for wearing and
removing personal protective equipment PPE in order to effectively protect against splatter, splash
and contamination (LO11).
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Section 9: Clinical Contact Surfaces (Clinical Application) Clinical Contact Surfaces are surfaces that can be contaminated via direct spray or spatter from dental
procedures, or via gloves on a DHCP.
A. The main sources of contamination are touch, transfer, and splash, spatter, and aerosol.
1. Touch: Surfaces that DHCP directly touch during procedures, and therefore potentially
contaminated. These surfaces include: dental unit controls, x-ray switches, chair
switches, chair side computers, computer mouse, light handles, cabinet handles, pens,
etc. Surfaces should be barrier protected or disinfected and cleaned between each
patient.
2. Transfer: Instrument trays and handpieces that are touched by contaminated
instruments or supplies. Surfaces should be barrier protected or disinfected and cleaned
between each patient
3. Splash, splatter, droplets: Oral fluids generated using the high and slow speed
handpieces, ultrasonic cleaners, and air/water syringe. At the minimum, surfaces that
may be contaminated via splash, spatter, or droplets must be disinfected after patient
treatment.
4. Aerosol: Fine, dust like particles generated while using high and slow speed handpieces,
ultrasonic cleaners, and air/water syringe. At the minimum, surfaces that may be
contaminated via s aerosol must be disinfected after patient treatment.
B. Methods for dealing with Surface Contamination: There are two methods that may be used
to deal with surface contamination, the barrier method, and precleaning & disinfecting
between patients.
1. Barrier Method: The barrier method entails using fluid resistant materials to prevent a
surface from becoming contaminated. Regulation states that a surface barrier must be
placed onto a surface that is difficult to clean and disinfect.
a. Surfaces that may be protected with barriers include:
1. Dental Chair Headrest & control buttons
2. Light handles & switches
3. Evacuator hoses & controls
4. X-ray controls & switches
5. Air-water syringe handles
6. Patient mirror handles
7. Drawer handles
8. Adjustment handles on operator and assistant chairs and tools
9. Bracket table
b. Requirements when using barriers:
1. After dental procedures surface barriers must be carefully removed so
the surface under the barrier does not become contaminated.
2. If the barrier moved or was torn during dental procedure and the
surface became contaminated the surface must be properly disinfected.
2. Precleaning & Disinfection: OSHA BBP Standard requires that contaminated work
surfaces be disinfected between each patient.
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a. Precleaning: Removal of blood, saliva, and other body fluids to prepare for
disinfection. Contaminated surfaces must be precleaned in order to be
effectively disinfected.
b. Disinfection: Kills disease-causing bacteria that remain on surfaces following
precleaning when correct procedures and guidelines are followed. Procudures
and guidelines for disinfection include (please note disinfecting does not kill
spores):
1. Clean and disinfect all clinical contact surfaces that are not protected by
impervious barriers using a California Environmental Protection Agency
(Cal/EPA) registered, hospital grade low- to intermediate-level
germicide after each patient.
2. The low-level disinfectants used shall be labeled effective against HBV
and HIV. Use disinfectants in accordance with the manufacturer's
instructions.
3. Clean all housekeeping surfaces (e.g. floors, walls, sinks) with a
detergent and water or a Cal/EPA registered, hospital grade
disinfectant.
4. Products used to clean items or surfaces prior to disinfection
procedures shall be clearly labeled and DHCP shall follow all safety data
sheet (SDS) handling and storage instructions.
5. Appropriate PPE during disinfecting procedures:
a) Gloves (heavy-duty utility) Exam gloves may not be used for
disinfecting.
b) Gowns
c) Masks
d) Protective Eyewear
6. Use proper category of disinfectant
a) Low-level.
1) This is the least effective disinfection process.
2) It kills some bacteria, some viruses and fungi
3) Does not kill bacterial spores or mycobacterium
tuberculosis var bovis.
b) Intermediate-level
1) Kills many human pathogens, including mycobacterium
tuberculosis var bovis
2) This process does not necessarily kill all spores
3) Used mainly for contaminated clinical surfaces and
items
c) High-level
1) Kills some, but not necessarily all bacterial spores.
2) This process kills mycobacterium tuberculosis var bovis,
bacteria, fungi, and viruses.
3) This is the process that the DBC requires if critical or
semi-critical instruments are heat sensitive
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A. Clinical Application & Competency
After completing Section 9, students will be able to Demonstrate proper disinfecting techniques for
clinical contact surfaces (LO12).
Section 10: Instrument Processing & Sterilization Proper processing of contaminated instruments and patient health care items is crucial in preventing
contamination and the spread of infection in the DHS. Therefore, it is important that dental assistants
understand and practice the correct processing methods.
A. Patient Care Item Classifications: The following are the Center for Disease Control and
Prevention (CDC) and the Dental Board of California (DBC) categories for patient care items
based on the risk of infection during their use:
1. Critical Instruments:
a. This class of instruments poses highest risk for infection if they are
contaminated with any microorganism.
b. Examples include surgical instruments, scalers, endo-instruments, bands,
brackets, wire and burs, and other items used to penetrate soft tissue or bone.
c. Most critical instruments are heat-tolerant and can be heat sterilized after each
patient.
2. Semi-critical Instruments:
a. While these instruments do not penetrate soft tissue or bone, they are used in
the patient’s mouth and therefore pose a risk of infection because they come in
contact with blood, saliva, and mucous membranes.
b. Examples include dental mouth mirrors, amalgam carriers, x-ray film holders,
dental hand pieces, and rubber dam forceps.
c. Most semi-critical instruments are heat-tolerant and may therefore be
sterilized. Those that are not heat-tolerant should receive high-level
disinfecting.
3. Non-Critical Instruments:
a. Non-critical items” are instruments, devices, equipment, and surfaces that come
in contact with soil, debris, saliva, blood, OPIM and intact skin, but not oral
mucous membranes.
b. Examples: Dental Chair, Dental Unit, X-ray Heads, Clinical Surfaces & Equipment,
and Curing light etc.
4. Disposable Items:
a. Single use items usually come in large packages and are labeled disposable or
single use.
b. Should be used on just one patient and then properly disposed of in order to
help reduce the chance of patient-to-patient contamination.
c. Examples of disposable items (prophy cups & brushes, Sterilization pouches,
Irrigating syringes, saliva ejectors, evacuation tips, surface barriers, face masks,
exam & surgical gloves, syringe needles, suture needles, plastic orthodontic
brackets.
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B. Transporting & Processing: The instrument processing area (sterilization area) should be in
an area easily accessible from all patient care areas in order to reduce the need to carry
contaminated instruments through clean areas of the office. Ideally, the processing areas
should be dedicated only to instrument processing, be separate from the operatories and
examination areas, and should absolutely not be in the path of common walkways. It should
contain separate areas for: (1) receiving, (2) preparation & packaging, (3) sterilization, and
(4) storage.
1. Receiving: The first step in instrument processing is the receiving area, contaminated trays
and disposable items are sorted; disposables are thrown into the regular trash.
Contaminated instruments are placed into the ultrasonic cleaner or holding solution.
a. Cleaning: Cleaning is removing visible debris (organic and inorganic) from
surfaces, dental instruments, and other objects through the use of water with
soap or enzymatic products.
b. Cleaning is required before sterilization and high-level disinfection to ensure
that there are no organic or inorganic materials on the surfaces of instruments
that may interfere with the effectiveness of the sterilization and/or disinfection
processes.
c. Ultrasonic cleaning: Soaking instruments in ultrasonic solutions loosen and
remove debris from instruments.
d. Instrument washing machines: Only FDA approved machines may be used to
disinfect patient care instruments. These machines subject contaminated
instruments to a level of heat that destroys some not all microorganisms.
2. Preparation & Packaging: Instruments should be wrapped or placed into pouches
designed for instrument sterilization prior to sterilizing to prevent re-contamination
once they have been sterilized. The following guidelines must be followed throughout
the preparation and packaging process:
a. Appropriate PPE must be worn when preparing and packaging contaminated
instrument for sterilization or high-level disinfections. The assistant must have
on all appropriate PPE:
1) Heavy duty nitrile utility gloves
2) Face mask
3) Eye protection
4) Fluid resistant gown
b. Heat-resistant critical or semi-critical instruments
1) Must be packaged in new (never reuse sterile pouches) or wrapped
before sterilization
2) Each package or wrapped instruments shall be labeled with the date of
sterilization and the specific sterilizer used if more than one sterilizer is
utilized in the facility
3) Each package must have a heat indicator or indicator tape on it
4) This heat indicator changes color when three things happen to the
pouch.
5) The pouch has come into contact with steam or chemical vapor
6) For a certain length of time
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7) Under a certain amount of pressure
8) The color change does not prove the instrument inside the package are
sterile
c. Heat-sensitive Critical or semi-critical instruments
1) Process with high-level disinfection and packaged or wrapped upon
completion of the disinfection process.
2) These instruments, items, and devices, shall remain sealed and stored in
a manner so as to prevent contamination, and shall be labeled with the
date of sterilization and the specific sterilizer used if more than one
sterilizer is utilized in the facility.
3) Each package must have a heat indicator or heat indicator tape on it.
3. Sterilization is the process that destroys all microorganisms including viruses, bacteria,
fungi and spores. Items are either sterile or they are not. All reusable items that come in
contact with patient blood, mucous membranes, or saliva must be heat-sterilized either
through steam, chemical vapor, or dry heat. Items that are heat-sensitive may be
cleaned using a high-level disinfectant. Any of the following methods will meet the
requirements for sterilization.
a. Steam: The processing of sterilizing instruments using steam under pressure.
The equipment used in steam sterilization is the autoclave or Statim.
b. Chemical Vapor: In chemical vapor sterilization, instruments are exposed to
chemical vapors including alcohol, formaldehyde, ketone, and acetone.
Formaldehyde is considered a carcinogen = can possibly cause cancer.
c. Dry Heat: Dry heat is the process of heating air high temperatures
approximately 375 degrees and transferring that air to the contaminated
instruments.
d. Spore test is the method to check correct functioning of sterilization cycle.
Spore testing should be verified for each sterilizer by the periodic use (at least
weekly).
1) Biological indicators (BIs) (i.e., spore tests) are the most accepted
method for monitoring the sterilization process because they assess it
directly by killing known highly resistant microorganisms
2) Spore test failure
3) The spore test should be repeated immediately after correctly loading
the sterilizer and using the same cycle that produced the failure.
4) sterilizer operating procedures should be reviewed, including packaging,
loading, and spore testing, with all persons who work with the sterilizer
to determine whether operator error
5) if there was no human error the sterilizer should remain out of service
until it has been inspected, repaired, or replaced
4. Storage: The final step in the process is the proper storage of sterilized instruments.
Upon completion of the heat sterilization process, instruments shall remain sealed and
stored in a manner so as to prevent contamination as follows:
a. Pouches of sterile instruments, when properly stored, may be stored for 6
months.
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b. After 6 months, the packages will be opened, instruments re-cleaned,
repackaged, and re-sterilized.
c. The package will be labeled with the date of sterilization and the specific
sterilizer used if more than one sterilizer is utilized in the facility.
d. Wrapped packages of sterile instruments, when properly stored, may be stored
for 30 days
e. After 30 days, the wrapped packages will be opened, instrument re-cleaned,
repackaged and re-sterilized.
f. The wrapped package will be labeled with the date of sterilization and the
specific sterilizer used if more than one sterilizer is utilized in the facility
g. Any package that is wet, torn, dropped on the floor, or damaged in any way
should not be used and must be re-cleaned, repackaged in new wrap, re-
sterilized, and re-labeled with the date of sterilization and the specific sterilizer
used if more than one sterilizer is utilized in the facility
C. Clinical Application & Competency
After completing Section 10, students will be able to perform disinfection and sterilization of patient
care items according to infection control guidelines (LO13).
Section 11: Dental Unit Waterlines (DUWLs) (Clinical Application) It is important to follow the DBC’s guidelines in order to prevent disease and cross-contamination in the
DHS.
A. Background: DUWLs can become colonized with microorganisms including bacteria, fungi,
and protozoa. These microorganisms can form a biofilm in the waterline tubing. Patients
with weakened immune systems who are exposed to these microorganisms are at risk of
infection.
B. Biofilm in Dental Waterlines: Biofilm is the “slime layer” that develops on surfaces due to
the bacterial cells in the water. Moisture and a suitable surface are all that is required for
biofilm to develop. The inside of dental tubing is an especially favorable location of biofilm.
C. Reducing Bacterial Contamination: There are several methods to use to reduce bacterial
contamination of water lines.
1. Flush waterlines for two full minutes at the start of each day, and 20 seconds between
patients.
2. Use a self-contained water reservoir system.
3. Use a self-contained water reservoir system combined with the application of chemical
germicides, as recommended by the waterline manufacturer.
4. Use a separate, sterile water system for surgical procedures
D. Clinical Application & Competency
After completing Section 11, students will be able to perform specific Dental Boards requirement to
maintain safe dental unit water lines (LO14).
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Section 12: Laboratory Infection Control Guidelines (Clinical Application) Items processed in the dental laboratory are sources of potential cross-contamination; therefore, it is
important to practice the following CDC’s guidelines when working in the lab:
A. Splash shields and equipment guards shall be used on dental laboratory lathes.
B. Fresh pumice and a sterilized or new rag-wheel shall be used for each patient.
C. Devices used to polish, trim, or adjust contaminated intraoral devices shall be disinfected or
sterilized, properly packaged or wrapped and labeled with the date and the specific sterilizer
used if more than one sterilizer is utilized in the facility.
D. If packaging is compromised, the instruments shall be recleaned, packaged in new wrap, and
sterilized again.
E. Sterilized items will be stored in a manner so as to prevent contamination.
F. All intraoral items such as impressions, bite registrations, prosthetic and orthodontic
appliances shall be cleaned and disinfected with an intermediate-level disinfectant before
manipulation in the laboratory and before placement in the patient's mouth. Such items
shall be thoroughly rinsed prior to placement in the patient's mouth.
G. Clinical Application & Competency
After completing Section 12, students will be able to demonstrate correct infection control
disinfection and protection strategies when performing lab procedures (LO15).
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GLOSSARY Barrier
A material object that separates a person from a hazard
Biohazard
Anything harmful or potentially harmful to humans, other species, or the environment
Biological indicators (BIs) (e.g., spore tests)
The most accepted method for monitoring the sterilization process because they assess it directly by
killing known highly resistant microorganisms
Bloodborne
Carried by the blood or found in the blood
Contamination
The presence of microorganisms on inanimate objects (clothing, surgical instruments) or in substances
(water, food, milk)
Cleaning
The removal of all foreign material (soil, organic debris) from objects
Common Vehicle
Contaminated material, product, or substance that serves as an intermediate means by which an
infectious agent is transported to two or more susceptible hosts
Communicable Disease
An illness due to a specific infectious agent that arises through transmission of that agent from an
infected person, animal, or inanimate reservoir to a susceptible host
Decontamination
The process (physical, chemical, or other means) of removing disease-producing microorganisms from
persons, spaces, surfaces, or objects
Dental Healthcare Personnel (DHCP)
Paid and non-paid personnel in the dental healthcare setting who might be occupationally exposed to
infectious materials, including body substances and contaminated supplies, equipment, environmental
surfaces, water, or air. DHCP includes dentists, dental hygienists, dental assistants, dental laboratory
technicians (in-office and commercial), students and trainees, contractual personnel, and other persons
not directly involved in patient care but potentially exposed to infectious agents (e.g., administrative,
clerical, housekeeping, maintenance, or volunteer personnel).
Dental Unit Water Lines (DUWL)
Small bore tubing usually made of plastic, used to deliver dental treatment water through a dental unit.
Disinfection
A process that results in the elimination of many or all pathogenic microorganisms on inanimate objects
with the exception of bacterial endospores
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Engineering Controls
Equipment, devices, or instruments that remove or reduce the risk of exposure to potentially infectious
material/s
Exposure Incident
When an employee has a specific splash of blood, saliva or other potentially infection material into the
eyes, nose, lips or mouth or a poke from a contaminated sharps to any part of the body
Exposure Control Plan
A written plan required by OSHA that describes how expposures to to bloodborne disease agents will be
controlled in a given work site.
High-level Disinfection
Kills bacteria, mycobacteria (TB), fungi, viruses, and some bacterial spores
Infectious Disease
A clinically manifest disease of man or animal resulting from an infection
Intermediate-level Disinfection
Kills bacteria, mycobacteria (TB), most fungi, and most viruses—does not kill resistant bacterial spores
Low-level Disinfection
Kills most bacteria, some fungi and some viruses. Will not kill bacterial spores and is less active against
some gram-negative rods (pseudomonas) and mycobacteria
Microorganism
Very small living organism unseen by eye (e.g. yeast, molds, viruses); may or may not cause disease
Pathogens or Infectious Agent
A microorganism capable of producing disease
Occupational Health Strategies
As applied to infection control, a set of activities intended to assess, prevent, and control infections and
communicable diseases in healthcare workers
Personal Protective Equipment (PPE)
Specialized clothing or equipment worn by a healthcare worker (HCW) for protection
Reservoir
Any person, animal, arthropod, plant, soil or substance (or combination or these) in which an infectious
agent normally lives and multiples, on which it depends primarily for survival, and where it reproduces
itself in such manner that it can be transmitted to a susceptible host
Sharps
Any object used or encountered in the clinical area of a dental office that may cause punctures or cuts
to any part of the body, including but not limited to; all contaminated broken glassware, syringes,
needles, scalpel blades, suture needles, disposable razors and other sharp instruments and items.
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Sterilization
A process that completely eliminates or destroys all forms of microbial life
Susceptible Host
A person or animal lacking effective resistance to a particular infectious agent
Tuberculosis (TB)
An infectious disease that is increasing in occurrence. It most commonly affects the lungs, but may
affect the GI and genitourinary tracts, bones, joints, nervous system, lymph nodes and skin as well.
Three types exist: human, bovine (cow) and avian (bird). Humans may become infected by all three
types, but in the U.S., the human type is most common
Transmission
Any mechanism by which a pathogen is spread by a source or reservoir to a person
Virus
The smallest organism that can be seen by an electron microscope a virus can only live inside a cell
where it reproduces itself. Viruses can cause disease immediately or can live in the cell for many years
before becoming active
Work Practice Controls
Controls that reduce or eliminate the likelihood of exposure by altering the manner in which a task is
performed.