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M a x w e l l ® 16 S y S t e M
A dvA n tAg es
Instruments
purIfIcatIon kIts
summary
orderIng InformatIon
resources
references
Maxwell® 16 SystemFast, simple, reliable automated DNA purification
✓ Use your time more wisely with decreased hands-on time thanks to an easy-to-use automated system.
✓ Consistently purify high quality DNA reducing the need for repeat purifications.
✓ Streamline your workflow with quick, 30-45 minute instrument run times.
✓ Match your purification needs to a Maxwell® 16 purification kit best suited to your sample type and downstream application.
M a x w e l l ® 16 S y S t e M
advantages
Instruments
inside mAxwell® 16
two formats
two Instruments
purIfIcatIon kIts
summary
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Inside Maxwell® 16Maxwell® 16 is a magnetic particle mover, not a a liquid handler
•gDNAiscapturedonmagneticparticlesandpurifiedthroughaseriesofcaptureandreleasewashesbeforefinalelution.
•Usingaparticlemovermeansfewerbreakdowns,clogsanddripsleadingtocross-contamination.
7683
TA
Magnets
Cartridges
1075
3MA
Sample
Sample Mixing
Particle Capture
DNA Binding
Particle Washing
DNA Elution
Magnet
Cartridge
Plunger
M a x w e l l ® 16 S y S t e M
advantages
Instruments
InsIde maxwell® 16
two formAts
two Instruments
purIfIcatIon kIts
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orderIng InformatIon
resources
references
6863
TA
8460
TA
Two Kit FormatsChoose the format best suited to your processing requirements.
Standard Elution Volume (SEV)300µl
•Largesampleprocessing•Lesspre-processing (variesbykit)
Low Elution Volume (LEV)50µl
•Smallsampleprocessing•Higheryield&concentration
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M a x w e l l ® 16 S y S t e M
advantages
Instruments
InsIde maxwell® 16
two formats
two instruments
purIfIcatIon kIts
summary
orderIng InformatIon
resources
references
Two Instrument Choices AvailableChoose between instruments with and without sample tracking
Both instruments provide:•30-45minuteprocessingtime•1to16samplesperrun•SEVandLEVkitcompatibility•Displaypanelandkeypad•12.8x17.3x12.9indimensions, 41.7lbweight
•1yearStandardwarranty, Premiumavailable
The AS3000 also provides:•Sampletracking/barcodereading•UVdecontamination•Touchscreen
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TA
Maxwell®16Instrumentas2000
Maxwell®16MDxInstrumentas3000
M a x w e l l ® 16 S y S t e M
advantages
Instruments
purIfIcatIon kIts
multiple choices
blood dna
lev blood dna
buccal swab lev dna
cell lev dna
tIssue dna
ffpe tIssue lev dna
ffpe plus lev dna
summary
orderIng InformatIon
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references
Maxwell® 16 Purification kit Blood
Buffy Coat Cells
Fresh Tissue
ffpe Tissue
Buccal swabs
Amniotic fluid
BloodDNA ✓ ✓
LEVBloodDNA ✓ ✓1 ✓
BuccalSwabLEVDNA ✓
CellLEVDNA ✓ ✓
tissue dna ✓
ffpe tissue lev dna ✓
FFPEPlusLEVDNA ✓
1RecommendedforbuccalsamplescollectedusingOmniSwabs.
Purification Kits to Match Your Sample TypeOptimized kits for reproducible extraction of high quality DNA
M a x w e l l ® 16 S y S t e M
advantages
Instruments
purIfIcatIon kIts
multIple choIces
blood dnA
lev blood dna
buccal swab lev dna
cell lev dna
tIssue dna
ffpe tIssue lev dna
ffpe plus lev dna
summary
orderIng InformatIon
resources
references
Kit Specificationskit format: sev
SampleInput: 50-400µlfreshorfrozenwholebloodcollectedinEDTA,heparinorACDtubes
Pre-processing: none
processing time: 36 minutes
Elutionvolume: 300µl
ExpectedYield: 8-15µgper400µl&4-9µgper200µl
ExpectedPurity: a260/A280ratios>1.8
comments: AlsosuitableforDNAisolationfrombuffycoatsamples.
Maxwell® 16 Blood DNA Purification KitConsistent gDNA isolation from fresh or frozen blood
1075
2TA
5650
TA
A.
B.
23kbM 1 2 3 4 5 6
M B1 2 3 4 5 6
5650
TA
A.
B.
23kbM 1 2 3 4 5 6
M B1 2 3 4 5 6
Isolation of Large, Intact gDNA from Fresh Blood
M 4003002001007550InputBloodVolume(µl)
Consistent Yields from 6 Donors
Consistent PCR Amplification of Human Growth Factor II Gene
M a x w e l l ® 16 S y S t e M
advantages
Instruments
purIfIcatIon kIts
multIple choIces
blood dna
lev blood dnA
buccal swab lev dna
cell lev dna
tIssue dna
ffpe tIssue lev dna
ffpe plus lev dna
summary
orderIng InformatIon
resources
references
Maxwell® 16 LEV Blood DNA Purification KitConsistent isolation of highly concentrated, pure gDNA
Kit Specificationskit format: lev
SampleInput: ≤400µlfreshorfrozenwholebloodcollectedinEDTA,heparinorACDtubes
Pre-processing: 20 minutes
processing time: 44minutes
Elutionvolume: 50µl
ExpectedYield: 8-10µgper300µlatconcentrationsupto200ng/µl
ExpectedPurity: a260/A280ratios>1.8
comments: Kitprocessesupto500µlofblood.AlsousedforisolationofDNAfrombuccalsamplescollectedonOmniSwabs.
0 50
100 150 200 250 300
Conc
entr
atio
n (n
g/l)
Tube Type
9274
MB
DNA
E SC SH E SC SH
E=EDTA SC=Sodium Heparin SH=Sodium Heparin
0.00
20.00
40.00
60.00
80.00
100.00
120.00
400 200 100 50
% Y
ield
9284
MB
(n=4)Input Blood volume (µl)
Highly Concentrated DNA Isolated from Commonly Used
Collection Tubes
Percent Yield Remains High Across All Input Volumes
M a x w e l l ® 16 S y S t e M
advantages
Instruments
purIfIcatIon kIts
multIple choIces
blood dna
lev blood dna
buccAl swAb lev dnA
cell lev dna
tIssue dna
ffpe tIssue lev dna
ffpe plus lev dna
summary
orderIng InformatIon
resources
references
Maxwell® 16 Buccal Swab LEV DNA KitConsistent isolation of highly concentrated, pure gDNA
Kit Specificationskit format: lev
SampleInput: BuccalsamplescollectedonPuritanMedicalDevicesswabs(1or2swabs)
Pre-processing: 25 minutes
processing time: 44minutes
Elutionvolume: 50µl
ExpectedYield: Averages~7µgperswabwithanaverageconcentrationof142ng/µl
ExpectedPurity: a260/A280ratios>1.8andA260/A230ratios>1.8
comments: AmountofDNAextracteddependsonthenumberofcellsperswab.
Donor Yield (µg)Conc.
(ng/µl) A260/A280
#1 6.80 136.05 1.90
#2 5.11 102.16 1.82
#3 8.75 174.96 1.89
#4 6.43 128.62 1.96
#5 7.45 148.91 1.95
#6 6.52 112.10 1.78
#7 6.52 130.38 1.78
Avg. 6.67 ± 1.2 133 ± -24 1.86 ± 0.1
High Yields and Purity
1075
4TA
DRB5 SSP Unitray System Analysis of gDNA Isolated from
Buccal Swabs
ResultspredictdonorcarriesDRB4allele0202(lanes11and12).Datamatchprevioustypingofthisdonor.
M a x w e l l ® 16 S y S t e M
advantages
Instruments
purIfIcatIon kIts
multIple choIces
blood dna
lev blood dna
buccal swab lev dna
cell lev dnA
tIssue dna
ffpe tIssue lev dna
ffpe plus lev dna
summary
orderIng InformatIon
resources
references
Maxwell® 16 Cell LEV DNA Purification KitIsolation of gDNA from cells and amniotic fluid
Kit Specificationskit format: lev
SampleInput: 10-10,000cellsor100µlor1mlofamnioticfluid
Pre-processing: ~5minutes(concentratinglargersamplevolumesbymicrocentrifugation)
processing time: 28minutes
Elutionvolume: 50µl
ExpectedYield: ~0.5ng(10cells)to~100ng(10,000cells)(lowamountsquantifiedbyqPCR)
comments:OptimizedforthepurificationofDNAfromlowcellnumbers.Alternatively,theMaxwell®16LEVBloodDNAKitcanbeusedforlargeramnioticfluidsamplesupto3ml.
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Maxwell® 16 Cell LEV DNA Purification Kit
Jurkat
Cell Number
Yiel
d (n
g)
1,000
100
10
1.0
0.1
0.011 10 102 103 104 105 106
HEK 293 HeLa
Volume Yield (ng)100µl ~3.7
1ml ~55
Examples of DNA Yields from Amniotic Fluid
Isolation of gDNA from Limiting Numbers of Cells
M a x w e l l ® 16 S y S t e M
advantages
Instruments
purIfIcatIon kIts
multIple choIces
blood dna
lev blood dna
buccal swab lev dna
cell lev dna
tissue dnA
ffpe tIssue lev dna
ffpe plus lev dna
summary
orderIng InformatIon
resources
references
Kit Specificationskit format: sev
SampleInput: ≤50mgoftissue
Pre-processing: none
processing time: 45minutes
Elutionvolume: 300µl
ExpectedYield: ~10-100µgdependingontissuetypeandamount
ExpectedPurity: a260/A280ratios>1.8
comments: Seemanualforadditionaldetails
Maxwell® 16 Tissue DNA Purification KitIsolation of gDNA suitable for downstream PCR-based assays
5655
TB
M Lung
23kb
5655
TD
M 1 3 5 B
Hair follicles (1,3, or 5) DNA purification with Maxwell® 16Tissue DNA Kit
PCR amplification of the Growth Factor II Gene
# Follicles
GF II amplicon
B = blank PCR reaction
Isolation of Intact gDNA fromHuman Lung Tissue
Isolation of gDNA from Human Hair Follicles Followed by Target Amplification
M a x w e l l ® 16 S y S t e M
advantages
Instruments
purIfIcatIon kIts
multIple choIces
blood dna
lev blood dna
buccal swab lev dna
cell lev dna
tIssue dna
ffpe tissue lev dnA
ffpe plus lev dna
summary
orderIng InformatIon
resources
references
Maxwell® 16 FFPE Tissue LEV DNA KitIsolation of amplifiable gDNA without harsh organic solvents
Kit Specificationskit format: lev
SampleInput: one to ten 5µm ffpe tissue sections
Pre-processing: OvernightproteinaseKdigestion
processing time: 28minutes
Elutionvolume: 50µl
YieldExamples: Spleen:2.8µgat56ng/µl,Kidney:2.4ugat47ng/µl,Brain:1.5µgat30ng/µl
ExpectedPurity: not determined
comments: ThiskityieldscleanerDNAthantheMaxwell®16FFPEPlusLEVDNAKit.
1075
5MA
Add Lysis Bufferand vortex
(1-10) 5µmFFPE sections
70° Covernight
Prep Purify Quantitate
Sample Yield (µg)Conc.(ng/µl)
Spleen 2.8 55.6Kidney 2.4 47.5Brain 1.5 30.1
Easy Protocol and Good Yields with the Maxwell® 16 FFPE Tissue LEV DNA Purification Kit
M a x w e l l ® 16 S y S t e M
advantages
Instruments
purIfIcatIon kIts
multIple choIces
blood dna
lev blood dna
buccal swab lev dna
cell lev dna
tIssue dna
ffpe tIssue lev dna
ffpe plus lev dnA
summary
orderIng InformatIon
resources
references
Maxwell® 16 FFPE Plus LEV DNA KitRapid isolation of more gDNA without harsh organic solvents
Kit Specificationskit format: lev
SampleInput: one to ten 5µm ffpe tissue sections
Pre-processing: 1hourorovernightproteinaseKdigestion
processing time: 28minutes
Elutionvolume: 50µl
YieldExamples: Spleen:3.6µgat72ng/µl,Kidney:6.1ugat122ng/µl,Brain:4.9µgat98ng/µl
ExpectedPurity: not determined
comments: ThiskityieldsmoreDNAthantheMaxwell®16FFPETissueLEVDNAKit.
1075
6MA
Add Lysis Bufferand vortex
(1-10) 5µmFFPE sections
70° C1 hr or overnight
Prep Purify Quantitate
Sample Yield (µg)Conc.(ng/µl)
Spleen 3.6 72.4
Kidney 6.1 122.2
Brain 4.9 98.3
Add Proteinase K
Solution
Results from 1 HourProteinase K Digestion
Shorter Protocol and Higher Yields with the Maxwell® 16 FFPE Plus LEV DNA Purification Kit
M a x w e l l ® 16 S y S t e M
advantages
Instruments
purIfIcatIon kIts
S U M M A RY
orderIng InformatIon
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references
SummaryAutomated gDNA purification streamlines workflow
•Optimized kits for all sample types: SuccessfullypurifygDNAfromblood,cells,tissues,buccalswabs,buffycoat,amnioticfluidandFFPEtissuesections.
•Fast, automated purification:30-45minuteinstrumentruntimesincreaseproductivitybyshorteningandautomatingDNAisolationportionofworkflow.
•High purity:ImprovedownstreamapplicationssuccesswithhighqualityinputgDNAtemplates
M a x w e l l ® 16 S y S t e M
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Instruments
purIfIcatIon kIts
summary
o r d e r i n g i n fo r m At i o n
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Ordering informationp r o d u c t s i z e c AtA l o g n u m b e r
Maxwel l® 16 Instrument Each as20001
Maxwel l® 16 MDx Instrument Each as30001
Maxwel l® 16 SEV Hardware Kit Each as12001
Maxwel l® 16 LEV Hardware Kit Each as12501
Maxwel l® 16 Blood DNA Kit 48preps as10101
Maxwel l® 16 LEV Blood DNA Puri f icat ion Kit 48preps AS12901
Maxwel l® 16 Buccal Swab LEV DNA Puri f icat ion Kit 48preps AS12951
Maxwel l® 16 Cel l LEV DNA Puri f icat ion Kit 48preps AS11401
Maxwel l® 16 T issue DNA Puri f icat ion Kit 48preps as10301
Maxwel l® 16 FFPE Tissue LEV DNA Puri f icat ion Kit 48preps as11301
Maxwel l® 16 FFPE Plus LEV DNA Puri f icat ion Kit 48preps as11351
r e l At e d p r o d u c t s s i z e c AtA l o g n u m b e r
Maxwel l® 16 LEV s implyRNA Cel ls K it 48preps AS12701
Maxwel l® 16 LEV s implyRNA Tissue Kit 48preps AS12801
Maxwel l® 16 LEV s implyRNA Blood Kit 48preps as13101
Maxwel l® 16 Viral Total Nucleic Acid Pur i f icat ion Kit 48preps as11501
1ForLaboratoryUse.OutsideoftheUnitedStates,thisproductisintendedforresearchuseonlyunlessotherwisestated.
M a x w e l l ® 16 S y S t e M
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Instruments
purIfIcatIon kIts
summary
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Resources
Additional Informationhttp://www.promega.com/maxwell16
Technical Manuals and DownloadsMaxwell®16InstrumentOperatingManual,TM295 »
Maxwell®16MDxInstrumentTechnicalManual,TM320 »
Maxwell®16BloodDNAKitTechnicalManual,TM284 »
Maxwell®16LEVBloodDNAPurificationKitTechnicalManual,TM333 »
Maxwell®16BuccalSwabLEVDNAPurificationKitTechnicalManual,TM333 »
Maxwell®16CellLEVDNAPurificationKitTechnicalBulletin,TB383 »
Maxwell®16TissueDNAPurificationKitTechnicalManual,TM284 »
Maxwell®16FFPETissueLEVDNAPurificationKitTechnicalBulletin,TB382 »
Maxwell®16FFPEPlusLEVDNAPurificationKitTechnicalManual,TM349 »
Promega Technical Services TeamContactTechnicalServiceswithanyquestionsregarding automatedDNAextraction.
M a x w e l l ® 16 S y S t e M
advantages
Instruments
purIfIcatIon kIts
summary
orderIng InformatIon
resources
r e f e r e n c es
References
ArticlesMandrekar,P.V.,et al.(2010)High-Concentration(>100ng/µl)GenomicDNAFromWholeBloodUsingMaxwell®16. PromegaCorporationWebsite.
Shenoi,H.,et al. (2007) Maxwell®16BuffyCoatGenomicDNAPurificationApplication. Promega Notes 97,6.
Wieczorek,D.,et al.(2008)AutomatedDNAPurificationfromOragene•DNA/SalivaSamplesUsingtheMaxwell®16System.PromegaCorporationWebsite.
PosterSchagat,T.et al.AutomatedgenomicDNApurificationfromformalin-fixedparaffin-embeddedtissueformicrosatelliteinstabilityanalysis.
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