MAXimum results. Minimum effort.MagMAX sample preparation for veterinary diagnostic testing

Sometimes purifying nucleic acid from animal samples can be like trying to pull a needle out of a haystack. Fortunately, the magnetic particle technology of the Applied Biosystems™ MagMAX™ sample preparation system makes purification convenient, flexible and efficient—enabling maximum results with minimum effort. The MagMAX sample preparation system offers the following benefits:

Efficiency• Fast and easy sample processing—

typically less than 30 min run time for 96 samples*

• Process up to 800 samples per day on a single instrument*

• Higher sensitivity with less sample input

Convenience and flexibility• One system purifies both DNA and RNA

from a wide variety of sample matrices

• Less hands-on manipulation

• There are Thermo Scientific™ KingFisher™ and Applied Biosystems™ MagMAX™ Express magnetic particle instruments available for any budget, application and throughput needed

Reliability • Less risk of cross-contamination

• Fewer false negatives due to effective removal of PCR inhibitors

• Helps improve the consistency of nucleic acid extraction yields

*Depending on sample type

MagMAX technologyThe MagMax sample preparation system is an integrated solution that accelerates, standardizes and automates the extraction of nucleic acid to prepare for downstream molecular analysis. Consisting of Applied Biosystems™ MagMAX™ nucleic acid isolation kits along with KingFisher or MagMAX Express magnetic particle processors, the MagMAX sample preparation system is designed to provide an integrated solution that offers unparalleled ease of use, shorter time-to-results and high-quality nucleic acid extraction.

The MagMAX sample preparation system can handle the most diffi cult challenges faced by veterinary diagnostic labs when extracting nucleic acid from biological fl uids and cell-free sample types, such as low concentrations of extractable nucleic acid, large processing volumes and sample complexity. And this is done without using organic solvents or nucleic acid precipitation, thus minimizing the challenges often encountered with fi lter-based methods (e.g., clogging, large elution volumes and inconsistent yields).

The MagMAX sample preparation system offers all of this, while minimizing the risk of cross-contamination.

Wide variety of sample matrices: Linear recovery of synthetic viral RNA from biological fl uids. MagMAX™ Lysis Buffer was added to samples of water, plasma, serum and milk, and the lysate was then spiked with a serial dilution of a 466 nt HIV Armored RNA™ transcript. Viral RNA was isolated using the MagMAX™ Viral RNA Isolation Kit. The recovered RNA was analyzed by qRT-PCR.

MagMAX achieves equal sensitivity with 10x less sample than competitor’s kit. Clinical tracheal and cloacal swab samples were collected from poultry infected with California exotic Newcastle disease (END). RNA was isolated from 50 μL of sample using the MagMAX™ protocol or 500 μL of samples using the NVSL validated protocol.

Performance MagMax Kit vs. Competitor’s kits. The MagMAX™ Pathogen RNA/DNA Kit outperforms competitor magnetic bead–based nucleic acid purifi cation kits. A high-titer PRRSV-positive serum sample was diluted to create mid-titer and low-titer samples. RNA was purifi ed from each dilution in triplicate following each supplier’s directions and using instrument scripts provided by each supplier. Purifi ed RNA was analyzed by qRT-PCR using PRRSV primers and probes. Lowest Ct

values were obtained with RNA purifi ed using the MagMAX Pathogen RNA/DNA Kit.

Convenience and fl exibility

ReliabilityGreater sensitivity than competitors’ kits

Effi ciency

MagMAX nucleic acid isolation kitsYears of research and development have produced a portfolio of nucleic acid isolation kits optimized to purify RNA and DNA from viruses, select bacteria and parasites found in sample matrices commonly submitted for veterinary diagnostic testing. The technology at the core of the MagMAX nucleic acid isolation kits:

• Binds RNA and DNA more effi ciently than silica-membrane methods

• Offers higher and more consistent nucleic acid yields

• Allows nucleic acids to be eluted in as little as 20 μL for manual extraction and in as little as 25 μL for automated extraction of nuclease-free water, resulting in more concentrated RNA and DNA

KingFisher and MagMAX Express Magnetic Particle ProcessorsThe family of KingFisher and MagMAX Express Magnetic Particle Processors are ideal for those veterinary diagnostic and research labs looking to automate their MagMAX sample preparation system.

For decades, KingFisher™ technology has been at the core of the industry’s best magnetic particle processors. Our proprietary magnetic separation technology lets labs process virtually any sample from any source, while minimizing the risk of cross-contamination. In short, KingFisher magnetic particle processors offer superior results in nucleic acid isolation with:

• Effective bead collection and transfer, which leads to superior washing, elution effi ciency and rapid processing

• Preprogrammed scripts optimized for each MagMAX kit and application

• A broad portfolio of instruments for any budget, application and throughput need

The family of KingFisher and MagMAX Express magnetic particle processors includes:

KingFisher Flex ProcessorOffering high-throughput or large processing volumes, the KingFisher Flex processor offers truly versatile nucleic acid purifi cation. Two separate magnetic heads provide 96- or 24-well formats and processing volumes ranging between 20 μL to 5,000 μL.

KingFisher Duo Prime ProcessorThe newest KingFisher™ instrument combines advanced functionality, mid-throughput capacity (up to 24 amples), and supports large processing volumes (up to 5 mL).*

KingFisher mL ProcessorProvides easy operation of up to 15 samples, including higher processing samples (50–1,000 μL).*

MagMAX Express Processor The original KingFisher system purifi es up to 24 samples (20–200 μL) at lower processing volumes.

MagMAX Pathogen RNA/DNA Kit

As a result, there is a MagMAX nucleic acid isolation kit to meet any lab’s unique needs, including:

MagMAX Pathogen RNA/DNA KitThe most versatile solution for veterinary diagnostic labs, providing high-throughput extraction of pathogen RNA or DNA from 50 μL to 300 μL input.

MagMAX-96 Viral RNA Isolation KitOffers high-throughput extraction of pathogen RNA or DNA from 50 μL input.

MagMAX-96 AI/ND Viral RNA Isolation KitValidated extraction of AIV and NDV RNA for NVSL network labs.

MagMAX Total Nucleic Acid Isolation KitProvides effective extraction of DNA from tough-to-lyse bacteria from feces that requires bead beating.

MagMAX Viral RNA Isolation KitUsed in high-volume, low-throughput applications, utilizing 1.5 mL tubes with a magnetic rack.

Other MagMAX kits are available from Thermo Fisher Scientifi c that may meet needs for laboratory applications outside of those described. For more information please go to thermofi sher.com

The MagMAX Pathogen RNA/DNA Kit WorkflowThe MagMAX Pathogen RNA/DNA KitA single kit that can accomodate:

• Purification of RNA and DNA

• Purification from viruses, bacteria and parasites

• Low and high sample input volumes

• A wide range of sample types

Workflow

VetMAX Reagents • Primers and probes diluted, premixed and optimized for efficient

nucleic acid amplification

• Internal positive control to monitor nucleic acid purification efficacy and PCR inhibition

• Quality reagents manufactured under ISO 9001 QMS conditions, providing confidence in the supply and quality of products

Master mixes• Amplifies in the presence of challenging PCR inhibitors

• Optimized for low copy number amplification of RNA and DNA targets

• Efficient amplification over 7 logs of RNA and DNA input amount

• Saves time and money by amplifying up to 4 different RNA targets simultaneously (multiplex kit, assay-dependent)

Applied Biosystems 7500 Fast Real-Time PCR Systems• Faster ramp rates yield rapid results without compromising assay quality

• Allows for more runs per day, with excellent performance equivalent to the 7500 system

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MagMAX Pathogen RNA/DNA Kit

KingFisher & MagMAX Express Magnetic Particle Processors

Sample type

Sample preparation

Applied Biosystems 7500 Fast Real-Time PCR System

• Add Lysis• Vortex• Spin

• Add Lysis• Disrupt• Spin

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Ordering information

Product Quantity Cat. No.

MagMAX kits

MagMAX Pathogen RNA/DNA Kit 480 or 5 x 96 rxns 4462359

MagMAX -96 Viral RNA Isolation Kit 96 rxns AM1836

MagMAX Total Nucleic Acid Isolation Kit 100 rxns AM1840

MagMAX -96 AI/ND Viral RNA Isolation Kit 384 rxns AM1835

MagMAX Viral RNA Isolation Kit 50 rxns AM1939

MagMAX -96 Viral RNA Isolation Kit 5 x 96 rxns AMB1836-5

KingFisher instruments and consumables

KingFisher Flex Magnetic Particle Processor with 96 Deep-Well Head 1 instrument 5400630

KingFisher Flex Magnetic Particle Processor with 24 Deep-Well Head 1 instrument 5400640

KingFisher Duo Prime Magnetic Particle Processor 1 instrument 5400110

KingFisher mL Unit 1 instrument 5400050

KingFisher Flex 96 Deep-Well Magnetic Head 1 head 24074430

KingFisher Flex 24 Deep-Well Magnetic Head 1 head 24074440

KingFisher Flex 24 Deep-Well Tip Combs and Plates 50 combs/plates 97002610

KingFisher Flex 24 Deep-Well Plates 50 plates 95040480

KingFisher 96 Tip Comb for KF Magnets 100 combs 97002534**

KingFisher 96 KF Microplates (200uL) 48 plates 97002540**

KingFisher Flex Microtiter Deep-Well 96 Plates 50 plates 95040460**

12-Tip Combs for KingFisher Duo Prime 50 combs 97003500

6-Tip Combs for KingFisher Duo Prime 48 combs 97003510

Elution Strips for KingFisher Duo Prime 40 strips 97003520

Combi Pack for KingFisher Duo Prime (tip combs, plates and elution strips) Pack (96 samples) 97003530

KingFisher mL Tip Combs 800 combs 97002111

KingFisher mL Tubes 900 tubes 97002121

KingFisher mL Combi Pack (tip combs and tubes) Pack (240 samples) 97002141

MagMAX Express instruments and consumables

MagMAX Express Magnetic Particle Processor 1 instrument 4400075

MagMAX Express Tip Combs 50 combs 4388452

MagMAX Express Plates 50 plates 4388474

MagMAX Express-96 Standard Tip Combs 100 combs 4388488

MagMAX Express-96 Standard Plates 48 plates 4388475*

MagMAX Express-96 Standard Magnetic Head 1 instrument 4388434

MagMAX Express-96 Deep Well Tip Combs 100 combs 4388487*

MagMAX Express-96 Deep Well Plates 50 plates 4388476*

MagMAX Express-96 Deep Well Magnetic Head 1 instrument 4388435

Find out more at thermofisher.com/animalhealth

For Veterinary Use Only. For In Vitro Use Only. Regulatory requirements vary by country; products may not be available in your geographic area. © 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. CO015967 0815

** Consumables are identical to comparable Applied Biosystems™ MagMAX™ Express consumables

* Consumables are compatible with KingFisher Flex deep-well unit

Ordering information

Product Quantity Cat. No.

Additional sample prep products

MagMAX Lysis/Binding Solution Concentrate 100 mL AM8500

MagMAX Wash Solution 1 Concentrate 205 mL AM8504

MagMAX Wash Solution 2 Concentrate 200 mL AM8640

Carrier RNA (1 mg/mL) 500 µL 4382878

RNA Rapid Extraction Solution 100–150 rxns AM9775

Magnetic-Ring Stand 1 each AM10050

Magnetic Stand-96 (24 pst magnets) 1 each AM10027

Single Place Magnetic Stand 1 each AM10026