Marine Biodiversity of Raja Ampat Islands - ibcraja4.orgibcraja4.org/assets/file/documents/PEER ANNUAL.pdf · i Marine Biodiversity of Raja Ampat Islands: the ARMS, Morphology, and

i

Marine Biodiversity of Raja Ampat Islands:

the ARMS, Morphology, and Genetic approaches for inventorying and monitoring patterns of marine

biodiversity

PEER Science Annual Report 1

st June 2012- 30

th April 2013

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Project Title:

Marine Biodiversity of Raja Ampat Islands: the ARMS, Morphology, and Genetic approaches for inventorying and monitoring patterns of marine biodiversity

Principal Investigator(s):

Abdul Hamid Toha

Institution(s):

State University of Papua

Reporting Period: June 2012-April 2013 Summary of Activities:

Overview: Outcomes from this project include establishing the Genetics Laboratory of UNIPA as a site for a field station, the digitization of voucher specimens, a project web, and for databases of marine biodiversity. Other outcomes from this research also include a manuscript of Conservation Genetics and a newsletter.

During the first period, research and laboratory equipment have been prepared and some field activities have been conducted. In Educational activities, we already held four workshops or training from June 1, 2012 through April 30, 2013 include the following: 1) Biodiversity, Molecular Ecology and Genetic Conservation, 2) Inventorying Marine Invertebrates and Microbes of Raja Ampat Islands, 3) Molecular Genetic Basics, and 4) Conservation Genetics. These courses had a total 129 participants from some institutions in Manokwari. We also support student’s field work practice in Raja Ampat along two months.

In Research, we already collected invertebrate specimens included water sample in order to collect bacteria samples. In total, there are 33 species invertebrate that identified in this period. Among them, two species has already analyzed and in preparation of writing article for publication. The researches affair had a total 23 participants from State University of Papua and Brawijaya University. Data base of these specimens have already prepared and will be published in our website. Research related to the one species of invertebrate which was T. gratilla from Raja Ampat has been done by graduate student in Brawjaya University. We also got a new research grant from State Ministry of Research and Technology of the Republic of Indonesia. Beside that we are part of a consortium who won new NSF PIRE award to continue our collaborative biodiversity research in whole Indonesia.

In Publications, we have already published 11 monthly Newsletters “Konservasi Biodiversitas Raja4” or “Raja Ampat Biodiversity Conservation” from June 2012 through April 2013. We also have a website in www.ibcraja4.org to socialize this project and other things related by conservations, biodiversity, Raja Ampat, laboratory and field methods. Book manuscript of “Diversity and Conservation of Sea Urchin” was in press for publication. Two articles have published in national journal. Beside that we were also preparing two articles else to published. We also socialized PEER sciences to all lecturers in UNIPA. The publications affair had a total 17 participants from State University of Papua and Brawijaya University.

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Events: We held four workshops or training from June 1, 2012 through April 30, 2013.

They are 1) training about Biodiversity, Molecular Ecology and Genetic Conservation on September, 2012 taught by Prof. Sutiman B. Sumitro, Luchman Hakim, Ph.D and Hamid Toha, 2) workshop the Field Work Practice for Inventorying Marine Invertebrates and Microbes of Raja Ampat Islands on December, 2012 taught by Hamid Toha and M. Dailami, 3) training Molecular Genetic Basics (DNA technology for Conservation) taught by Hamid Toha and Kuncoro, and 4) general lectures of Conservation Genetics on April, 2013 taught by Hamid Toha. These courses had a total 129 participants from different institutions in and outside UNIPA. We also support undergraduate student’s field work practice in Raja Ampat along two months. This field work had a total 10 participants from UNIPA. As a way of research building capacity for young faculty and students, our project had facilitated the faculty and students to join in research at Raja Ampat. Six participants are now conducting research with focus on invertebrates of Raja Ampat. In addition, we support undergraduate, master and doctoral students in research for their thesis and dissertation. These events had a total 7 participants from UNIPA and UB. Below is the detail of the workshops. Number of workshops

Number of conferences

Number of exchange visits with U.S. partners

4 0 0

Date of workshop, conference, or visit

Location of workshop, conference, or visit

Title of workshop or conference or name of visitor

Number of female participants

Number of male participants

April 11-12, 2013

UNIPA, Manokwari

Training of Molecular Genetic Basics

9 8

April 11, 2013 UNIPA, Manokwari

General Lecture of Conservation Genetics

28 31

March- July 2013

UNIPA and Brawijaya University

Support in research for thesis and dissertation

3 4

Dec, 2012-Peb 2013

Raja Ampat Field Work Practice 8 2

Dec 2012- March 2013

UNIPA Call for proposal (Capacity building in research)

5 1

December 19-20, 2012

UNIPA, Manokwari

Inventory of Raja Ampat’s Invertebrates Biodiversity; Work Field Practice Workshop

9 13

September 10-12, 2012

UNIPA, Manokwari

Biodiversity, Molecular Ecology and Genetic Conservation Workshop

15 16

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Educational Impacts:

Researchers, students, and faculty from different backgrounds and institutions in and outside UNIPA get benefit from our project through three kinds of the activities (training and teaching, research, and publication). Educational curricula have been developed especially for Genetic subject in Fishery Department. We also add a practicum in existing subject course in Molecular Biology and Genetics for multi faculties in UNIPA. Formal educational activities included 4 new courses.

We also had facilitated the faculty and student to join research as a way of research building capacity for young faculty and students in UNIPA and UB. Our project also supported undergraduate, master and doctoral students in research for their thesis and dissertation. Educational Status Number of

Females Number of Males

Undergraduate (BS) 5 10

Masters Student (MS or M Phil) 0 1

Doctoral Student (PhD) 1 0

Postdoctoral scholars 0 0

Other junior collaborators 5 1

Laboratory Technicians 2 3

Other participant involved in our project was detailed in Supplementary information.

Infrastructure Development:

In this period, we got a new research grant from State Ministry of Research and Technology of the Republic of Indonesia. The title of research is ”Seleksi dan Domestikasi Kandidat Indukan Udang Galah Macrobrachium rosenbergii (de Man, 1879) asal Papua berdasarkan Marka Genetik untuk Pengembangan Indukan Baru”. This research began on January to December 2013. The team of this research is Robi Binur (Head, UNIPA), Adi Pancoro (Researcher, Bandung Institute of Technology) and Abdul Hamid Toha (Researcher, UNIPA). Beside that we are part of a consortium who won new NSF PIRE award to continue our collaborative biodiversity research in whole Indonesia. The proposal “PIRE: Assembly of Marine Biodiversity Along Geographic and Anthropogenic Stress Gradients” was funded by the U.S. National Science Foundation. The grant funding period will commence on January 1, 2013. Duration program is five years from 2013-2015. In this period we have already bought a camera for documentation, laptop for server database and website. We also purchased new micropipettes for training and teaching. We used these equipments when the training was running. The equipments also used in research of student and faculty who wants to finishing their study and conduct individual research.

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Below is a list the number of new grants received as a result of PEER Science funding.

International Local (in PI’s country)

Number of research grants received

1 1

Number of patent applications submitted

0 0

We have not yet a patent applications until now. However, we are publishing our book manuscript that will get a copyright. The manuscript was not fully funded by PEER but PEER have already made this happen as soon as possible.

Publications: We already published a monthly newsletter. Until now, we have published eleven editions of newsletters from June 2012-April 2013. For socializing of our project with the program, we also launched a website at www.ibcraja4.org (look at Supplementary Information). We are also preparing manuscript three books and three articles that related to our project but not include in our proposal before. Books

1. Toha, A.H.A. Molecular Genetics (Revision Edition). For submission to AlfaBeta Publisher, Bandung. (in Bahasa)

2. Toha, A.H.A., Sumitro, S.B., Hakim, L., Widodo. “Diversity and Conservation of Sea Urchin”. For submission to publisher. (in review)

3. Toha, A.H.A., Mahardika, A.P.W. “Sea Urchin Tripneustes gratilla”. For submission to publisher. (in Bahasa)

Articles 4. Toha, A.H.A, Sumitro, S.B., Hakim, L., Widodo. Genetic connectivity of sea urchin

Tripneustes gratilla in Cenderawasih Bay, Papua-Indonesia. For submission to International journal (in review).

5. Toha, A.H.A., Sumitro, S.B. L. Hakim., Widodo. 2012. Kondisi habitat bulu babi Tripneustes gratilla (Linnaeus, 1758). Berk. Penel. Hayati 17 (2): 139-145.

6. Toha, A.H.A., Permana, A., Sumitro, S.B. L. Hakim., Widodo. 2012. Penentuan jenis kelamin bulu babi Tripneustes gratilla secara morfologi. Berk. Penel. Hayati 17 (2): 211-215.

Type of Technical Publication

International Publications

Local publications (in PEER PI’s country only)

Number of joint publications with U.S. PEER partner

Journal articles 1 2

Proceedings 1

Technical reports

Books 3

Book chapters

Book editor

We are also preparing one paper about spiny lobster Panulirus versicolor for national journal entitled: Genetic structure of Lobster Panulirus versicolor in Raja Ampat waters.

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Technical Research Presentations:

We attended four conferences and seminars of national or international in June to September 2012. First, we attended a national seminar (4th Biodiversity National Seminar) in Airlangga University at Surabaya, East Java on September 15, 2012. We presented a paper “Genetic diversity of Sea Urchin Tripneustes gratilla (Linnaeus 1758) in Papua”. Second, we presented a paper “Genetic diversity pattern of Tripneustes gratilla in Indonesia”. The paper was presented at Indonesian Biodiversity Research Center Synthesis Meetng in Udayana University at Denpasar on Augusts 7, 2012. Third, we presented Marine protected areas network, a mtDNA studies for conservation planning: A case study of Tripneustes gratilla. This paper was presented at the 3rd International conference on global resources conservation in Brawijaya University at Malang on July 7, 2012. Lastly, we present Comparative genetic diversity pattern of Tripneustes gratilla in the Indonesian Archipelago and Indo-Pacific at Coral Triangle Partnerships for International Research and Education synthesis meeting in Manila (Philippines) on June 16, 2012.

I also presented a paper “Education and Research for Biodiversity in Raja Ampat and Indonesia”. Short Review: PEER, PIRE, IBRC, NESCent, etc. in State University of Papua on April 12, 2013. This seminar was done for monitoring and evaluation our project for full year.

Number of technical research presentations made

5

Linkages and Potential Development Impacts:

We attended a PEER Financial Workshop that held by USAID in Jakarta on

November 20-21, 2012. In this workshop, we shared our activities and financial management with other institutions that invited by USAID. We also get important things from this workshop in financial management.

When we held the training in UNIPA, we have any linkages developed with different backgrounds and institutions outside UNIPA. They are from government agencies in our country (Center of Research and Development of Agricultural Papua Barat, Center of Research and Development of Forestry Province Papua Barat, National Park of Teluk Cenderawasih agency, and Ministry of Marine and Fishery Province Papua Barat), from private companies (STKIP Muhammadiyah, local private university in Manokwari), and Community groups or nongovernmental organizations (Conservation International-Indonesia). We intend to expand this work in the future especially in working of biodiversity etc.

Our project was visited by IBTC team (International Business and Technical Consultant Inc.) for monitoring and evaluating the Indonesian Biodiversity Research Center (IBRC) on November 2-3, 2012. Besides that team of Directorate of Bilateral Foreign Funding, National Development Planning Agency (BAPPENAS) of Republic of Indonesia was also visit us on December 10, 2012. Although they weren’t coming solely for PEER project, however, they also got information about PEER project from us. These important for us and the project to anticipate what will happen in the future.

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This project was also result in significant benefits for national co-operation and collaboration with Brawijaya University, as an state institution poised to be a resource for scientific collaboration in Indonesia. This partnership creates a strong platform for research and a direct mechanism into actionable conservation. We also have started to work together with Warren Wilson College, US, in partnership on educational and research at Raja Ampat.

Organization Yes No

USAID Yes

Government agencies in your country Yes

Community groups or nongovernmental organizations Yes

Private companies Yes

Problems Encountered: We ordered ARMS equipments from US then ARMS shipped from Jakarta to

Sorong (West Papua) since 2012. However, the ARMS deployment in Raja Ampat waters is not yet running until now. The ARMS will be deployed at the Raja Ampat waters adjust with US our partner’s schedule on summer. Because of ARMS will keep in a full year in bottom of the sea, we think to get the results of ARMS will need more time. This condition will make any problems we may have encountered in making progress on our project objectives.

To resolve the problem, we have to deploy the ARMS in this summer with or without our U.S. partners. We also tried to conduct research with other method (without ARMS). By this method we have already collected many specimens from Raja Ampat. I think we have to run the same ways again in the next period. Although ARMS results will be taken, the data of this method will still function and could be compared with ARMS products.

We also have difficulties related by processing ISSN for our monthly newsletter. Until now, our monthly newsletters have no ISSN. We also have difficulties related by publishing our books. Recently, UNIPA press (UNIPA publisher) did not work again and we could not get a support letter for publishing ISSN and ISBN. Therefore, we interest to set up a publisher for supporting our publications. The publisher will function and keep on both in the short term and in the long term.

Plans for Activities During the Coming Year:

We intend to deploy ARMS at Raja Ampat waters adjust with our US partner’s schedule on this summer. We also intend to conduct lab researches with selected young researcher from our invitation in call for proposal. In addition, we will publish activities and some of the result from field work practice in posters form, and we will upload in website and newsletter. We also planned to publish our two books and three articles. We want to make a copy of our published book and available free for all on our website.

We intend to held field work with the US students and faculty in Raja Ampat on May, 2013. We will work together with lecturers of Brawijaya University, professor from

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Warren Wilson College who will run a field trip in Raja Ampat. Our team will conduct research about conservation and will collect specimen of invertebrate. Some travel will run in the second year of this project.

The project is now preparing the second year molecular genetics course, there are 35 participants from different institutions in and outside UNIPA anticipated to join 2013. Furthermore, in the second year of the project we increasing both the number of courses taught and the number of students involved. We also will expand our research projects by partnering with various local and international institutions and project personnel that have submit their proposal through the national and international competitions. Additional Comments:

We intend to revise our budgeting to maximize our project workings. This is including revise in other direct costs, for instance, publication costs, workshops and conferences, and digitizing collection. We have no allocate budget for these item before. The fund will be taken from equipment’s fund that not fully used.

Supplementary Information: Below is supplementary information of our project.

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TRAINING AND TEACHING 1. Training Workshop: Biodiversity, Molecular Ecology and Genetic

Conservation

Agenda

Day 1 Time Activities Presenter Moderator

09:00 – 09:10 Welcome Ketua panitia Committee

09:10 – 09:30 Review of MB-RAI A.Hamid Toha Committee

09:30 – 09:45 Speech Rektor Committee

09:45 – 10:00 Break

10:00 – 11:15 Molecular Ecology 1

Prof. Sutiman B. Sumitro, SU, D.Sc (UB)

Hamid

11:15 – 12:30 Biodiversity 1

Luchman Hakim, M.AgrSc, Ph.D (UB)

Ema

12:30 – 13:15 Break

13:15 – 14:00 Extraction Method of DNA Hamid Toha

14:00 – 15:00 Amplification Method (PCR) Hamid Toha

15:00 – 15:15 Break

15:15 – 16:00 GenBank Hamid Toha


09:00 – 09:30 MB-RAI website Jeni, M.Si/M. Dailami Panitia

09:30 – 09:45 Review of ctPIRE A.Hamid Toha Panitia

09:45 – 10:00 Break Panitia

10:00 – 11:15 Biodiversity 2

Luchman Hakim, M.AgrSc, Ph.D (UB)

Ema

11:15 – 12:30 Molecular Ecology 2

Prof. Sutiman B. Sumitro, SU, D.Sc (UB)

Sabarita

12:30 – 13:15 Break

13:15 – 14:00 Agarose Electrophoresis Method

Hamid Toha

14:00 – 15:00 Clean up amplification product method

Hamid Toha

15:00 – 15:15 Break

15:15 – 16:00 Cycle sequencing Hamid Toha


09:00 – 09:45 Aricle writing techniques Luchman Hakim, M.AgrSc, Ph.D (UB)

Committee

09:45 – 10:00 Break Committee

10:00 – 11:00 Conservation Genetics

Widodo, M.Si, Ph.D.Med.Sc/A. Hamid Toha

Jeni

11:00 – 12:00 Genetic analysis

Widodo, M.Si, Ph.D.Med.Sc./A. Hamid Toha

Sabarita

12:00 – 13:15 Break

13:15 – 14:00 Mega5 Hamid Toha

14:00 – 15:00 DNAsp 5.10 Hamid Toha

15:00 – 15:15 Break

15:15 – 16:00 Arlequin Hamid Toha

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Training Workshop: Biodiversity, Molecular Ecology and Genetic Conservation

2. Debriefing Field Work Practice for Inventorying Marine Invertebrates and Microbes of in Raja Ampat Islands.

Agenda

Time

Activities Titles Presenter

Wednesday, Desember 19, 2012

09.00 – 09.30

Opening Head of Fishery Departmen, FPPK

09.30 - 10.30

Debriefing and Introducing research program

Teknik Pengambilan Sampel Iinvertebrata dan Mikroba untuk Data Morfologi dan Genetik

A.Hamid A. Toha

10.30 – 11.30

Panduan Penulisan Laporan PKL dan Kriteria Penilaian PKL

Simon P.O. Leatemia, S.Pi, M.Si

11.30 - 12.30 Dinamika Sosial Masyarakat Pesisir dan Etika Penelitian di Masyarakat

Nurhani Widiastuti, S.Pi, M.Si

Tuesday, Desember 20, 2012

09.00 – 10.00

Presentation of Mangrove group

1. Inventarisasi dan Pemanfaatan Jenis-Jenis Mangrove oleh Masyarakat Lokal di Kabupaten Raja Ampat

2. Komposisi Jenis dan Kerapatan Mangrove di Kampung Amdui Selat Sagawin Kabupaten Raja Ampat

3. Inventarisasi jenis-jenis mangrove di Pulau Kaki Distrik Manokwari Utara Kabupaten Manokwari

Sampari Suruan Harry Silalahi Maryo B. Mandacan

10.00 – 11.00

Presentation of Makrozoobentos & Mollusca at Mangrove groups

1. Inventarisasi Makrozoobenthos di Ekosistem Mangrove pada Kampung Amdui Distrik Selat Sagawin Kabupaten Raja Ampat

2. Asosiasi moluska pada ekosistem Mangrove di Kampung Amdui Selat Sagawin Kabupaten Raja Ampat

3. Asosiasi Gastropoda pada

Putri Ngilawane Deasy Yamco

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Ekosistem Mangrove di Kampung Sawinggrai Distrik Meosmanswar Kabupaten Raja Ampat

4. Kelimpahan jenis Bia Kodok (Polimesoda spp) di Kampung Amdui Distrik Selat Sagawin Kabupaten Raja Ampat

David Lomban Steven Ayomi

11.00 – 12.00

Presentation of groups of sea cucumber, Kima and Sepuncula

1. Inventarisasi Jenis-Jenis Teripang Hasil Tangkapan dan Pemanfaatan oleh Nelayan

2. Kelimpahan Jenis Teripang di Kampung Sawinggrai Distrik Meosmanswar Kabupaten Raja Ampat

3. Kelimpahan Jenis Kima (Tridacnidae) di Kampung Amdui Selat Sagawin Kabupaten Raja Ampat

4. Sebaran dan Pemanfaatan Filum Sipuncula di Kampung Amdui Distrik Selat Sagawin Kabupaten Raja Ampat

Bayu Pranata Ronald Hambuako Absalom Wakum Rico R. Mailissa

12.00 – 13.00

Break

13.00 – 14.00

Presentation of Group of Social economy, aquaculture and fisihing

1. Analisis Hasil Pendapatan usaha budidaya ikan lele dumbo dengan system kolam di Distrik Masni dan Distrik Prafi

2. Sasi di Kofiau Kabupaten Raja Ampat

3. Budidaya kerang mutiara (Pinctada sp) di Kampung Amdui Distrik Selat Sagawin Kabupaten Raja Ampat

4. Konstruksi Alat Tangkap Jaring Payang di Kabupaten Manokwari

Siti Mariyam Erick Wanggai Hugo Rumbewas Wanny Kambu

14.00 – 15.00

Presentation of group of seaweed and Macroalgae

1. Inventarisasi Jenis-jenis Makroalga di Kabupaten Raja Ampat

2. Komposisi dan Kelimpahan Jenis Lamun di Kabupaten Raja Ampat

3. Inventarisasi jenis-jenis lamun di Pulau Kaki Distrik Manokwari Utara Kabupaten Manokwari

Marlon Iwanggin Yerri Mundoni Lidia Kandipi

15.00 – 16.00

Presentation of groups of Mollusc and fish at seaweed

1. Asosiasi dan Komposisi Ikan pada Ekosistem Lamun di Distrik Amdui Kabupaten Raja Ampat

2. Kelimpahan Jenis-Jenis Gastropoda Dan Bivalvia (Molusca) pada Ekosistem Lamun di . Kampung Amdui Distrik Selat Sagawin Kabupaten Raja Ampat

3. Kelimpahan jenis Gastropoda dan

Jefri Arizona M. Fajar Marsaoly Yulius Tata

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Bivalvia (Moluska) pada ekosistem Lamun di Kampung Kapisawar Distrik Meosmanswar Kabupaten Raja Ampat

4. Komposisi jenis ikan yang berasosiasi pada hamparan Lamun di Pulau Mansinam

Verbelina Baransano

Closing

Debriefing Practice Field Work for Inventorying Marine Invertebrates and Microbes of in Raja Ampat Islands.

3 & 4 Genetic Basics, General Course and Training Genetic Basic

Agenda

Time Activities Presenter

Thursday, April 11, 2013

09.00 – 09.30 Opening Head of Fishery Departmen, FPPK

09.30 – 11.30 General Course: Conservation Genetics Abdul Hamid Toha

11.30 –12.00 Discussion Moderator

13.00 – 14.00 Meeting Fishery Department

14.00 – 16.00 Genetic Basics Abdul Hamid Toha

Friday, April 12, 2013

09.30 - 10.30 Socialization of PEER project Abdul Hamid Toha

14.00 – 16.00 Practicum: Extraction, PCR, Electrophoresis Andry Kuncoro

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General Course

Training Genetic Basics

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RESEARCH Field Work

No Species n Ekstraction PCR Seq Note

1 Acteon tornalitis 3 No No No No

2 Actinopyga palauensis 1 No No No No

3 Atilia ocelata 3 No No No No

4 Bohadsia marmorata 4 No No No No

5 Bolmaguttata millegranosa 1 No No No No

6 Casidula vespertillionis 4 No No No No

7 Chicoreus capucinus 3 No No No No

8 Euapta godeffroyi 5 No No No No

9 Fissilabia decollate 3 No No No No

10 Herpetopoma atrata 3 No No No No

11 Hippopus hippopus 3 No No No No

12 Holothuria fuscopuntata 1 No No No No

13 Littorina cocinea 5 No No No No

14 Littorina scabra 3 No No No No

15 Littorina sp 3 No No No No

16 Nerita costata 4 No No No No

17 Nerita undata 3 No No No No

18 Nerita planespira 3 No No No No

19 Opheodesoma sp 5 No No No No

20 Panulirus versicolor 42 Yes Yes Yes, 32 No

21 Polymesoda spp 4 No No No No

22 Sipunculus nudus 5 No No No No

23 Stichopus variegates 1 No No No No

24 Telebraria palustris 7 No No No No

25 Telescopion-telescopion 1 No No No No

26 Terebrallia sulcata 3 No No No No

27 Tripneutes gratilla 11 Yes Yes Yes, 11 No

28 Tridacna crocea 3 No No No No

29 Tridacna squamossa 3 No No No No

30 Tridacna maxima 3 No No No No

31 Tridacna gigas 3 No No No No

32 Turritella simplex 1 No No No No

33 Vitoida auqatilis 4 No No No No

151

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Euapta godeffroyi

E. godeffroyi distributed in Raja Ampat coastal. Habitat of E. godeffroyi is

Intertidal pool, in sand, beneath stones. Ecology: benthic, continental shelf, detritus

feeder, deposit feeder. This species inhabit in depth range 0-77 m. E. godeffroyi or paraer in local name at Raja Ampat was found in rocky or sandy mud in Waigeo

Island, Raja Ampat

E. godeffroyi. Kingdom Animalia. Phylum Echinodermata. Class Holothuroidea. Order

Apodida. Family Synaptidae. Genus Euapta. E. godeffroyi from Waigeo Island

Specimens in UNIPA Genetic Lab

No ID Sampel Species Sites n Ext PCR Seq 1 UNP.000 E. godeffoyi P. Batanta Raja Ampat 5 No No No

ID Sample

No ID Sampel Collected Date Locality Exist in Containers 1 UNP.000.01 08-feb-13 P. Batanta LGU Box R4 2 UNP.000.02 08-feb-13 P. Batanta LGU Box R4 3 UNP.000.03 08-feb-13 P. Batanta LGU Box R4 4 UNP.000.02 08-feb-13 P. Batanta LGU Box R4 5 UNP.000.03 08-feb-13 P. Batanta LGU Box R4

Fissilabia decollata

F. decollata distributed in Indonesia, including in Raja Ampat. These neatly

spiraled snails sometimes seen, usually in large numbers on rocky shores and seawalls on our Southern shores. Elsewhere, they are called cluster winks for their habit of

gathering in clusters. But disperse to feed at high tide. The size was about 2cm. The

snails brood their young. Fertilization is internal and fertilized embryos are stored in a

brood pouch behind the head of the female. They are then released into the sea as free-swimming larvae.

F. decollata found between rocks of Mangroves in Waigeo Island, Raja Ampat. F.

decollata. Kingdom Animalia. Phylum Mollusca. Class Gastropoda. Order Neogastropoda. Family Planaxidae. Genus Fissilabia.

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No ID Sampel Species Sites n Ext PCR Seq 1 UNP.000 F. decollata P. Batanta Raja Ampat 3 No No No

ID Sample

No ID Sampel Collected Date Locality Exist in Containers 1 UNP.000.01 08-feb-13 P. Batanta LGU Box R4 2 UNP.000.02 08-feb-13 P. Batanta LGU Box R4 3 UNP.000.03 08-feb-13 P. Batanta LGU Box R4

Herpetopoma atrata

H. atrata were distributed in Indonesia including in Raja Ampat water. It preferred intertidal rocky reefs as its habitat and have size 15–20mm.

H. atrata found in sandy mud (loam) and Mangrove, Batanta Island, Raja Ampat. H.

atrata. Kingdom Animalia. Phylum Mollusca. Class Gastropoda. Order Archaegastropoda. Family Trochidae. Genus Herpetopoma.


No ID Sampel Spesies Lokasi n Eks PCR Seq 1 UNP.0040 H. atrata Raja Ampat 3 No No No

ID Sample


Holothuria fuscopunctata

H. fuscopunctata was also found in Raja Ampat. This species is deep

creases in the thick body wall and distinct fine spots easily distinguish this large

holothurian from others. H. fuscopunctata is more common in water deeper than about

10 metres on sand or a mixture or rubble and sand. It occurs at the outer barrier reefs

as well as other mid-shelf reefs.

H. fuscopunctata foun in rocky/sandy mud, Waigeo Island, Raja Ampat. H.

fuscopunctata. Kingdom Animalia. Phylum Echinodermata. Class Holothuroidea. Order Aspidochirotida. Family Holothuriidae. Genus Holothuria.

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No ID Sampel Spesies Lokasi n Eks PCR Seq 1 UNP.0039 H. fuscopuntata P. Batanta Raja Ampat 3 No No No

ID Sample


Hippopus hippopus

Hippopus is a genus of large tropical saltwater clams, marine bivalve mollusks in

the family Tridacnidae, the giant clam family. This species distributed in Raja Ampat coastal. The shell of this clam is very thick with prominent ribs, and has reddish

blotches making it easy to differentiate from other clams. The mantle of these clams does not extend past the edge of the shell, and are brownish-green to gray in color with faint gold stripes. The large opening in the mantle, which is called the incurrent aperture, lacks the tentacles that are common with other clams within the Tridacnidae family.

Kima H. hippopus in rocky coral Batanta Island, Raja Ampat. H. hippopus. Kingdom

Animalia. Phylum Mollusca. Class Bivalvia. Order Veneroida. Family Tridacnidae. Genus Hippopus.


No ID Sampel Spesies Lokasi n Eks PCR Seq 1 UNP.0019 H. hippopus P. Batanta Raja Ampat 3 No No No

ID Sample


Tridacna sp

Tridacna sp. features a deeply folded, almost zig-zag dorsal shell margin,

reminiscent of the distantly related T. gigas. This character allows for an easy distinction from the two Red Sea congeners, the fluted clam (T. squamosa Lamarck 1819) and Tridacna maxima Ro¨ding 1798. The latter two species show only weakly

sinusoidal dorsal shell margins. The species occurs throughout the Indonesia area including in Raja Ampat. Live specimens were found exclusively in very shallow water

17

including reef flat, sea grass beds, sandy-rubble flats, on slight depressions in barren rocky flat, or under branching corals orcoral heads, shallower than 2 m.

(a) T. costata (b) T. maxima (c) T. squamosa Tridacna sp in rocky coral in Batanta Island, Raja Ampat. T. costata. Kingdom

Animalia. Phylum Mollusca. Class Bivalvia. Order Veneroida. Family Tridacnidae. Genus Tridacna. Specimens in UNIPA Genetic Lab

No ID Sampel Species Sites n Ext PCR Seq 1 UNP.0021 T. crocea P. Batanta Raja Ampat 3 No No No No ID Sampel Species Sites n Ext PCR Seq 1 UNP.0017 T. squamosa P. Batanta Raja Ampat 3 No No No No ID Sampel Species Sites n Ext PCR Seq 1 UNP.0018 T. maxima P. Batanta Raja Ampat 3 No No No No ID Sampel Spesies Sites n Ext PCR Seq 1 UNP.0020 T. gigas P. Batanta Raja Ampat 3 No No No

ID Sample

No ID Sampel Collected Date Locality Exist in Containers 1 UNP.0021.01 08-feb-13 P. Batanta LGU Box R4 2 UNP.0021.02 08-feb-13 P. Batanta LGU Box R4 3 UNP.0021.03 08-feb-13 P. Batanta LGU Box R4 No ID Sampel Collected Date Locality Exist in Containers 1 UNP.0017.01 08-feb-13 P. Batanta LGU Box R4 2 UNP.0017.02 08-feb-13 P. Batanta LGU Box R4 3 UNP.0017.03 08-feb-13 P. Batanta LGU Box R4 No ID Sampel Collected Date Locality Exist in Containers 1 UNP.0018.01 08-feb-13 P. Batanta LGU Box R4 2 UNP.0018.02 08-feb-13 P. Batanta LGU Box R4 3 UNP.0018.03 08-feb-13 P. Batanta LGU Box R4 No ID Sampel Collected Date Locality Exist in Containers 1 UNP.0020.01 08-feb-13 P. Batanta LGU Box R4 2 UNP.0020.02 08-feb-13 P. Batanta LGU Box R4 3 UNP.0020.03 08-feb-13 P. Batanta LGU Box R4

Panulirus versicolor

P. versicolor (Latreille, 1804) is one of the six varieties of spiny lobster that found in the Indonesian Archipelago including in Raja Ampat waters. The other five being P. homarus, P. longipes, P. penicillatus, P. polyphagus and P. ornatus (Girsang et al 1995). P. versicolor grows up to 40 centimetres. Like all spiny lobster, this animal

18

has no claws, two spiny rostra over the eyes, and two pairs of large antennae. Both antennae are usually white with the first pair are double ended and the second hard and spiny. The carapace is white, pink and black, with horizontal bands and a reticulate.

P. versicolor. Kingdom animalia, Phylum arthropoda, subphylum Crustacea, Class

Malacostraca, Ordo Decapoda, Family Palinuridae, Genus Panulirus.

Telescopium telecospium

T. telescopium is commonly seen in Indonesia mangroves including in Raja Ampat, on

mud, sometimes in the hundreds covering a large area. This large snail has a tall,

conical shell strongly reminiscent of an ice cream cone. Features of T. telecospium are

8-15cm. The Operculum of Telescopium is small and circular. The animal is velvety

black with a highly extendible proboscis. There is a third eye on its mantle margin, in

addition to a pair of eyes at the tentacles. It can stay out of water for long periods of

time. Telescopium feeds on detritus and algae from the mud surface at low tide by

sucking using its proboscis (Reid et al., 2008).

T. telescopium found in Mangrove and Mud, Batanta Island, Raja Ampat. T.

telescopium. Kingdom Animalia. Phylum Mollusca. Class Gastropoda. Order Sorbeoconcha. Family Potamididae. Genus Telescopium. Tripneutes gratilla

T. gratilla, a sea urchin widely distribution throughout the Indo-Pacific including in Raja Ampat. This species is ecologically important, especially in sea grass habitats

and is a food source with good potential for aquaculture. Habitat of T. gratilla are

sheltered and moderatley exposed reef. They can reach 10 to 15 centimetres (4 to 6 in) in size. They are dark in colour, usually bluish-purple with white spines. The pedicles are also white, with a dark or black base. The spines of some specimens are wholly orange, while those of others are only orange-tipped or completely white. This colour disappears when the individual dies or is taken out of the ocean, and is difficult to preserve.

19

T. gratilla. Kingdom Animalia. Phylum Echinodermata. Class Echinodea. Order Temnopleuroida. Family Toxopneustidae. Genus Tripneutes.

Vitoida auqatilis

V. auqatilis, found on Mangrove leaves and muddy loam in Batanta Island, Raja

Ampat. V. auqatilis. Genus Vitoida.

Littorina sp Height commonly exceeding 5 mm, and in some species slightly exceeding 1.5

cm; operculum horny ; periostracum not uniformly tan or brown (Almagorda subrotundata is brown, but usually has spiral banding ) (mostly at higher tide levels on rocly shores, or on rocks, concrete and wood in bays , sometimes in salt marshes). The periwinkles are found on the seashore in the littoral zone and sub littoral zone in all parts of the Raja Ampat.

Littoraria scabra, common name: the mangrove periwinkle, is a species of sea

snail, a marine gastropod mollusk in the family Littorinidae, the winkles or periwinkles. The size of an adult shell varies between 15 mm and 40 mm. This species is distributed

in Indonesia including in Raja Ampat. Littoraria coccinea is a species of sea snail, a

marine gastropod mollusk in the family Littorinidae, the winkles or periwinkles.

Littorina sp, found on Mangrove leaves and muddy loam in Batanta Island, Raja Ampat

20

Lab Work

PCR Products

21

Sequencing Results

Data Analyses

22

Presentation

23

PUBLICATIONS

1. Website: http://www.ibcraja4.org/

24

2. Monthly Newsletters: http://ibcraja4.org/page/read/newsletters

June 2012 July 2012 August 2012

September 2012 October 2012 November 2012

December 2012 January 2012 Pebruary 2013

http://ibcraja4.org/page/read/newsletters

25

March 2012 April 2013

3. Article 1: http://berkalahayati.org/index.php/bph/article/view/642

26

Article 2: http://berkalahayati.org/index.php/bph/article/view/658

27

4. Cover of Book Manuscript (in Press)

Abdul Hamid A. Toha

Sutiman B. Sumitro

Luchman Hakim

Widodo

KEANEKARAGAMAN DAN

KONSERVASI BULU BABI

28

Information on the numbers and types of students and other junior collaborators involved in the project.

No Name (Gender)

Title Department

(Faculties), Goal

Undergraduate (Field Trip)

1 Jefri Arizona (Male)

Asosiasi dan Komposisi Ikan pada Ekosistem Lamun di Distrik Amdui Kabupaten Raja Ampat

Fishery (Faculty of Husbandry, Fishery and Marine Sciences-FPPK) Field Work

2 David Lomban (M)

Komposisi Jenis Gastropoda pada Ekosistem Mangrove di Kampung Sawinggrai Distrik Meosmanswar Kabupaten Raja Ampat

Fishery (FPPK), Field Work

3 Rico Ricardo Mailisa (M)

Sebaran dan Pemanfaatan Filum Sipuncula di Kampung Amdui Distrik Selat Sagawin Kabupaten Raja Ampat


4 Hugo Rumbewas (M)

Budidaya kerang mutiara (Pinctada sp) di Kampung Amdui Distrik Selat Sagawin Kabupaten Raja Ampat


5 Putri P. Ngilawane (Female)

Inventarisasi Makrozoobenthos di Ekosistem Mangrove pada Kampung Amdui Distrik Selat Sagawin Kabupaten Raja Ampat


6 Deasy Yamco (F)

Asosiasi gastropoda pada ekosistem Mangrove di Kampung Amdui Selat Sagawin Kabupaten Raja Ampat


7 Absalom Wakum (M)

Kelimpahan Jenis Kima (Tridacnidae) di Kampung Amdui Selat Sagawin Kabupaten Raja Ampat


8 Ronald Hambuako (M)

Kelimpahan Jenis Teripang di Kampung Sawinggrai Distrik Meosmanswar Kabupaten Raja Ampat


9 Steven Ayomi (M)

Kelimpahan jenis Bia Kodok (Polimesoda spp) di Kampung Amdui Distrik Selat Sagawin Kabupaten Raja Ampat


10 Yulius Tata (M)

Kelimpahan jenis Gastropoda dan Bivalvia (Moluska) pada ekosistem lamun di Kampung Kapisawar Distrik Meosmanswar Kabupaten Raja Ampat


Other junior collaborators-Capacity Building in Research

1 Nurhani Widiastuti,S.Pi, M.Si (F)

Partisipasi masyarakat local dalam pengelolaan ekowisata di Kampung X Raja Ampat

Fishery (FPPK, UNIPA), Capacity building in research

2 Fanny Simatauw, S.Pi, M.Si (F)

Partisipasi masyarakat local dalam pengelolaan ekowisata di Kampung X Raja Ampat


29

3 Juliana Leiwakabessy, S.Pi, M.Si (F)

Ekologi Molekuler Ketam Kenari (Birgus Latro L.) di Raja Ampat dan Manokwari


4 Ida Lapadi, S.Pi, M.Si (F)

Ekologi Molekuler Ketam Kenari (Birgus Latro L.) di Raja Ampat dan Manokwari


5 Tresia Tururaja,. S.Ik, M.Si (F)

Identifikasi Kima (Tridacna) Dengan Menggunakan Pendekatan Metode DNA Barcoding dan Variasi Genetik di Perairan Raja Ampat Papua Barat


6 Robi Binur, S.Si, M.Si (M)

Seleksi dan domestikasi kandidat indukan udang galah Macrobachium rosenbergii (de Man, 1879) asal Papua berdasarkan marka genetik untuk pengembangan indukan baru.

Biology (Faculty of Sciences, UNIPA), Capacity building in research

Undergraduate (Research)

1 Kuncoro (M) Identifikasi spesies laut menggunakan pendekatan DNA barcoding

Marine Sciences, Thesis

2 Nur Aisyah Hasniar (F)

Variasi Warna Menunjukkan Keragaman Genetik Teripang (Holothuridea) Di Perairan Raja Ampat

Biology (Faculty of Sciences), Thesis

3 Bayu Pranata (M)

Bioekologi dan filogeni ketam kenari Raja Ampat

Fishery (FPPK), Thesis

4 Masriana (F)

Identifikasi kima (Tridacna) dengan menggunakan DNA barcoding dan variasi genetik di Perairan Raja Ampat, Teluk Doreri dan Teluk Wondama

Marine Sciences, Thesis

5 Herimiel Ambrauw (M)

Kajian kearifan local dalam bentuk penerapan sasi hasil laut di Distrik Kofiau, Kabupaten Raja Ampat

Fishery Sciences, Thesis

Master Students

6 Veince Silahooy (M)

Distribusi spasial dan keragaman genetik bulu babi (Tripneustes gratilla) di Pulau Ambon dan Raja Ampat

Biology (Faculty of Sciences, Brawijaya University), Thesis

Doctoral Students

7 Jumiati Mustiah (F)

Eksistensi sosial-lingkungan dalam ekowisata di Kepulauan Raja Ampat

Social Economy (Agriculture Faculty of UNIPA-Brawijaya University), Dissertation

30

Laboratory Technicians, Newsletter contributors, Website operators

1 Jehan Haryati, S.Si, M.Si (F) Admin staff Biology Graduated student, UB

2 Q. Mohammed (M) IT staff Biology Graduated student, UB

3 M. Dailami, S.Si (M) Admin/technician Chemistry Graduated (Faculty of Sciences, UNIPA), Capacity building in research

4 Andyka AriWibowo, S.Ik (M) Technician Marine Sciences Graduated, Thesis

5 Nurhani Widiastuti, S.Pi, M.Si (F)

Admin staff Faculty of UNIPA

6 Prof. Sutiman B. Sumitro, D.Sc

Consultant, Trainer

Faculty of Brawijaya University

7 M. Takdir Newsletter contributor

Faculty of UNIPA

8 Widodo, Ph.D Researcher, Coauthor


9 L. Hakim, Ph.D Researcher, Trainer


10 Jeni, S.Pd, M.Si Researcher Faculty of UNIPA

11 Hamid Toha PI, Trainer Faculty of UNIPA

12 Robi Binur Contributor in newsletter

Faculty of UNIPA

13 Yuliana L, S.Pi, M.Si Contributor in newsletter

Faculty of UNIPA

14 Irma A., S.Ik, M.Si Contributor in newsletter

LIPI staff

15 Hermawaty A., S.Si, M.Si Contributor in newsletter

Faculty of UNIPA

16 Lutfi Contributor in newsletter

Faculty of UNIPA

17 Kuncoro Technician UNIPA student

Documents

Marine Biodiversity of Raja Ampat Islands - ibcraja4.orgibcraja4.org/assets/file/documents/PEER ANNUAL.pdf · i Marine Biodiversity of Raja Ampat Islands: the ARMS, Morphology, and