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The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
BSD City – Tangerang, Indonesia – 24th October, 2019
i
Background
Southeast Asia is one of biodiversity hot spots in the world. For example, Indonesia is
one of the 17 megadiverse countries and with 2 of the world‟s 25 biodiversity hotspots. Wide
variation of climate and numerous islands in the region also increased the biodiversity of the
region. This high biodiversity can potentially provide rich bioresources and genetic resources.
These biodiversity resources are expected to contribute to the sustainable development of the
region.
JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) was launched in 2015
to develop a platform of three fields, energy and environment, bioresources and biodiversity
and disaster prevention, for Japan-ASEAN science and technology cooperation to achieve
sustainable development in collaboration with various stakeholders in ASEAN and Japan.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
BSD City – Tangerang, Indonesia – 24th October, 2019
ii
Organizing Committee:
General Coordinator : Dr. Dede Heri Yuli Yanto
Dr. Joeni Setijo Rahajoe
Prof. Mamoru Kanzaki
Mr. Ryuichi Fukuhara
Advisory Board : Prof. Dr. Sulaeman Yusuf
Prof. Dr. Gono Semiadi
Prof. Dr. Andria Agusta
Prof. Takashi Watanabe
Dr. Yukako Monda
Dr. Ruliyana Susanti
Technical Committee Chairman : Dr. Didi Tarmadi Secretariat Dita Meisyara, S. Si
Riska Surya Ningrum, M. Sc
Anggi Permatasari, S.Si
Program : Dr. Sukma Surya Kusumah
Dr. Khoirul Himmi Setiawan
Dr. Ikhsan Guswenrivo
Dr. Maya Ismayati
Evana, M.Sc
Bernadeta Ayu Wdyaningrum, M.Si
Kartika Dyah Palupi, M.Med.Sc
Treasury and meal : Dwi Ajias P, M. Si
Listiana Oktavia, M. Si
Logistic and
accommodation : Yusup Amin, M.Si
Anugrah Fajar, S. T
Ahmad Fathoni, M. Si
Website : Ikut Tri Handoyo, M.Kom
Eko Widodo, S.T
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
BSD City – Tangerang, Indonesia – 24th October, 2019
iii
Agenda
Time Agenda Speakers Moderator
(PIC)
07.00-8.00 Registration MC
OPENING CEREMONY
08:10-08:20 Welcome Remarks-1 Prof. Dr. Enny Sudarmonowati
Deputy of Life Science, LIPI
08:20-08:30 Welcome Remarks-2 Ms. Emi Kaneko
Japan Science and Technology Agency
(JST)
08:30-08.40 Welcome Remarks-3 Prof. Yasuyuki Kono
Leader of Japan-ASEAN Scientific,
Technology and Innovation Platform
(JASTIP) and Vice President, Kyoto
University
KEYNOTE SPEAKER
08.40-09.10 Dr. Ir. Arifin Rudianto, M.Sc
Deputy for Maritime Affairs and
Natural Resources, BAPPENAS
Prof. Takashi
Watanabe
09.10-09.40 IPBES Assessments on
Bioodiversity and Ecosystem
serveces and Toword Post
2020 Framework and
Achievement of SDGs
Dr. Noriaki Sakaguchi
Senior Advisor, Global Environment
Department, Japan International
Cooperation Agency (JICA)
09.40-09.55 Discussion
09.55-10.00 Photo Session
10.00-10.30 Break and Poster Session
SESSION I - Bioresources and Biodiversity
10.30-10.50 Invited speaker
“Emerging threats of invasive
ants to native pollinator
community: insight into virus
spillover”
Dr. Lin Chun-Yi
Kyoto University
Dr. Ikhsan
Guswenrivo
10.50-11.00 Discussion
11.00-11.10 Oral presentation 1
“Honey, a persisting NTFP on
a deforested peat landscape of eastern Sumatra”
Dr. Ahmad Muhammad
Riau University
11.10-11.20 Oral presentation 2
“A Preliminary Study on The
Population and Habitat of
Saltwater Crocodile
Ms. Grace Serepina Saragih
Research and Development Center for
Environmental Quality and Laboratory
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
BSD City – Tangerang, Indonesia – 24th October, 2019
iv
(Crocodylus porosus) in Timor Island, East Nusa Tenggara”
11.20-11.30 Oral presentation 3
“High Corolla Colour
Variation of Hoya coronaria in
Belitung Island: Potential Use
and Conservation”
Dr. Sri Rahayu
Bogor Botanical Garden-LIPI
11.30-11.40 Oral presentation 4
“Exploration of Indonesian
Nutmeg Seed and Its Active
Compound for Management of
Arthritis Inflammation”
Dr. Yanti
Atma Jaya Catholic University of
Indonesia
11.40-11.50 Oral presentation 5
“Health Percentage of Coral
Reef In Tunda island, Banten Province”
Dr. Mujiyanto
Research Institute for Fisheries
Enhancement
11.50-12.00 Oral presentation 6
“Development of Bamboo
Propagation for Source
Material of Bamboo-based
Activated Carbon”
Dr. Riksfardini Ermawar
Research Center for Biomaterials, LIPI
12.00-13.00 Lunch / Poster session
SESSION II - Bioresources Utilization and Technology
13.00-13.20 Invited speaker
“Biocomposites from Natural
Fibers”
Prof. Subyakto
Research Center for Biomaterials, LIPI
Prof. Dr. Kenji
Umemura
13.20-13.30 Discussion
13.30-13.40 Oral presentation 7
“Wood Quality of
Kelempayan and Benuang
from East Kalimantan,
Indonesia: Fiber Quality and
Juvenile-Mature Boundary”
Ms. Sari Delviana Marbun
IPB University
13.40-13.50 Oral presentation 8
“The Effects of FeCl3
Concentration on Hydrothermal Pretreatment of
Oil Palm Fronds to Enhance
Reducing Sugar Production”
Dr. Sunardi
Lambung Mangkurat University
13.50-14.00 Oral presentation 9
“The Potential for Using Corn
Husk as Agricultural Waste for
Particleboard Raw Material”
Mr. Kurnia Widji Prasetiyo
Research Center for Biomaterials, LIPI
14.00-14.10 Oral presentation 10
“The Effect Application of
Organic Fertilizer and
Dolomit Toward Cassava
Production In Banten Province-Indonesia”
Dr. Viktor Siagiaan
Assessment Institute for Agricultural
Technology of Banten
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
BSD City – Tangerang, Indonesia – 24th October, 2019
v
SESSION III - Bioprospecting and Sustainable Use of Bioresources
14.10-14.30 Invited speaker
“Balancing economic
development and biodiversity
protection – Biodiversity value
chains & certification of
products ”
Mr. Elpidio V. Peria
ASEAN Centre for Biodiversity
Prof. Hiroshi
Kamitakahara
14.30-14.40 Discussion
14.40-14.50 Oral presentation 11
“Bioconversion of artemisinin
by endophytic fungi associated
Artemisia sp. grown in
Indonesia and Japan”
Dr. Shoji Maehara
Fukuyama University
14.50-15.00 Oral presentation 12
“Isolation of Bioactive
Compounds from hyptis suaveolens (l.) Poit and Its
Antioxidant Activity”
Dr. Swe Swe Aye
Yangon University, Myanmar
15.00-15.10 Oral presentation 13
“Cosmeceutical Potency of
Functional Ripe Cider Buni”
Ms. Sthefanie Marchellia
Atma Jaya Catholic University of
Indonesia
15.10-15.30 Break/poster session
SESSION IV - Access and Benefit sharing: Lesson learnt from ASEAN countries
15.30-15.50 Invited speaker
ABS: Experiences and lessons
learned from Sarawak
Ms Margarita Naming
Senior Research Officer, Sarawak
Biodiversity Centre, Malaysia
Dr. Ruliyana
Susanti
15.50-16.10 Invited speaker Access and benefit sharing:
lesson learnt from Mycotech
Mr. Erlambang Ajidarma
Mycotech Indonesia
16.10-16.20 Discussion
CLOSING CEREMONY
16.20-16.25 Closing remarks Mr. Takeshi Miyazawa
First Secretary, Japan
Representative to ASEAN
16.25-16.30 Closing remarks Dr. Iman Hidayat
Director of Research Center for
Biomaterials, LIPI
.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
BSD City – Tangerang, Indonesia – 24th October, 2019
vi
Foreword
Good Morning,
On behalf of the organizing committee, I would like to say welcome to all
participants, especially Dr. Ir. Arifin Rudianto, M.Sc- Deputy for Maritime Affairs and
Natural Resources, BAPPENAS (National Plan/Policy on Bioresources-based Sustainable
Development) and Dr. Noriaki Sakaguchi, Senior Advisor, Global Environment Department,
Japan International Cooperation Agency (JICA) as Keynote speakers, and also Dr. Lin Chun
Yi- Kyoto University; Prof. Subyakto-Research Center for Biomaterials- Indonesian Institute
of Sciences (LIPI); Mr. Elpidio V. Peria- ASEAN Centre for Biodiversity; Ms Margarita
Naming, Senior Research Officer, Sarawak Biodiversity Centre, Malaysia; and Mr.
Erlambang Ajidarma, Mycotech Indonesia as Invited speakers. I also would like thanks Prof.
Dr. Enny Sudarmonowati-Deputy of Life Science- Indonesian Institute of Sciences (LIPI); Ms.
Emi Kaneko Japan Science and Technology Agency (JST); Prof. Yasuyuki Kono Leader of
Japan-ASEAN Scientific, Technology and Innovation Platform (JASTIP) and Vice President-
Kyoto University; and Mr. Takeshi Miyazawa, First Secretary, Japan Representative to
ASEAN.
7th JASTIP Symposium is organized by the JASTIP (Japan-ASEAN Science,
Technology and Innovation Platform), Kyoto University, Research Center for Biomaterials -
LIPI, Research Center for Biology-LIPI. The theme of this year is “Biodiversity, Genetic
Resources and Innovative Bioresource Technology: Toward 2025 Biodiversity sustainable
use.” This symposium is attended by 200 participants from some countries, i.e: Japan,
Malaysia, Myanmar, Philippines, and Indonesia. We received more than 70 abstracts for both
oral and poster presentations that vary in the broad scope of bioresources and biodiversity,
bioresources utilization and technology, and bioprospecting. The selected papers will be
published in IOP Conference Series indexed by Scopus.
I would like to express my deepest gratitude and sincere appreciation to all speakers,
presenters, and participants for their valuable contributions. I wish to express my special
thanks to all committee members who have spent significant time for the sake of the
conference successful. Hopefully, we will have a fruitful and memorable meeting in the
symposium, and also we can strengthen and develop our collaboration.
Thank you very much.
Prof. Mamoru Kanzaki
On behalf of Organizing Committee
Kyoto University
October 21, 2019
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
BSD City – Tangerang, Indonesia – 24th October, 2019
vii
Table of Contents
Background ........................................................................................................................................ i
Organizing Committee ....................................................................................................................... ii
Agenda ............................................................................................................................................. iii
Foreword ...........................................................................................................................................vi
Table of Contents ..............................................................................................................................vii
KEYNOTE SPEAKER ....................................................................................................................... 1
Curriculum Vitae: Dr. Ir. Arifin Rudianto, M. Sc ............................................................................. 2
IPBES Assessments on Bioodiversity and Ecosystem serveces and Toword Post 2020
Framework and Achievement of SDGs Noriaki Sakaguchi........................................................... 5
INVITED SPEAKER .......................................................................................................................... 6
Emerging threats of invasive ants to native pollinator community: insights into virus spillover
Chun-Yi Lin and Chin-Cheng Yang ................................................................................................... 8
Biocomposites from Natural Fibers Subyakto, Nanang Masruchin and Sukma Surya
Kusumah .......................................................................................................................................... 11
Balancing economic development and biodiversity protection – Biodiversity value chains &
certification of products Peria, Elpidio; Schlegel, Bianca; Dr. Simorangkir; Dicky .................... 13
Successful Experience and Gaps in Access and Benefit Sharing (ABS) through Research on
Traditional Knowledge in Sarawak, Malaysia Margarita Naming and Yeo Tiong Chia .............. 16
Mycelium-Composite as Future Biomaterial Erlambang Ajidarma ............................................ 18
ORAL PRESENTATION .................................................................................................................. 19
A Preliminary Study on The Population and Habitat of Saltwater Crocodile (Crocodylus
porosus) in Timor Island, East Nusa Tenggara Grace Serepina Saragih, Kayat, Muhammad
Hidayatullah , Dani Sulistyo Hadi .................................................................................................... 21
High Corolla Colour Variation of Hoya coronaria in Belitung Island: Potential Use and
Conservation Sri Rahayu & Yulian Fakhurrozi ............................................................................ 22
Exploration of Indonesian Nutmeg Seed and Its Active Compound for Management of
Arthritis Inflammation Yanti, Yustine, Marlin Elza, and Hans Christian Leman ........................ 23
Health Percentage Of Coral Reef In Tunda Island, Banten Province Mujiyanto and Joni
Haryadi ............................................................................................................................................ 24
Development of Bamboo Propagation for Source Material of Bamboo-based Activated
Carbon Riksfardini A. Ermawar, Widya Fatriasari, Rikno Harmoko, N. Sri Hartati, A. Dany
Sunandar, Ratih Damayanti, Saptadi Darmawan ............................................................................ 25
Wood Quality of Kelempayan and Benuang from East Kalimantan, Indonesia: Fiber Quality
and Juvenile-Mature Boundary Sari Delviana Marbun, Imam Wahyudi, Jajang Suryana,
Deded Sarip Nawawi ........................................................................................................................ 26
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
BSD City – Tangerang, Indonesia – 24th October, 2019
viii
The Effects of FeCl3 Concentration on Hydrothermal Pretreatment of Oil Palm Fronds to
Enhance Reducing Sugar Production Nurwahdah, Rodiansono, A Kamari, R Abdullah,
Sunardi ............................................................................................................................................ 27
The potential for using corn husk as agricultural waste for particleboard raw material
Kurnia Wiji Prasetiyo, Lekianus Zalukhu, Lilik Astari and Dede Hermawan ................................. 28
The Effect Application of Organic Fertilizer and Dolomit Toward Cassava Production in
Banten Province-Indonesia Viktor Siagian, Ulima Amanda, Eko Kardiyanto ............................. 29
Bioconversion of artemisinin by endophytic fungi associated Artemisia sp. grown in Indonesia
and Japan Shoji Maehara, Ahmad Fathon, Muhammad Ilyas, and Andria Agusta ................... 30
Isolation of bioactive compounds from Hyptis suaveolens (L.) Poit. and its antioxidant activity
Swe Swe Aye, Khin Thant Sin .......................................................................................................... 31
Cosmeceutical Potency of Functional Ripe Cider Buni Sthefanie Marchellia, Raymond
Rubianto Tjandrawinata, and Yanti ................................................................................................. 32
POSTER SESSION .......................................................................................................................... 33
Diversity of Epipelic Diatom in Telaga Pengilon Dieng, Indonesia Kenanga Sari and Tri
Retnaningsih Soeprobowati ............................................................................................................. 34
Lipase Production by Paecilomyces sp. BK1 on Olive Oil I Made Sudiana, Atit Kanti, Idris ..... 35
Forest Management Prospects Through Development Non-Timber Forest Products
Gusmailina & Suwardi Sumadiwangsa ........................................................................................... 36
Chemical Characterization of Wood Vinegar Obtained from Acacia Bark Arief Heru
Prianto, Budiawan, Yoki Yulizar and Partomuan Simanjunta ......................................................... 37
Optimization of Pulp Concentration for Bioethanol Production from Elephant Grass
(Pennisetum purpureum) with Addition Tween 20 and Using Two Commercial Yeasts Ina
Winarni and Dwi Wahyuni ............................................................................................................... 38
Two Chemotypes of Sindora leiocarpa Essential Oil and Evaluation of Its Antimicrobial and
Antioxidant Activity Dewi Wulansari, Kartika Dyah Palupi, Praptiwi, Hiroshi Kamitakahara,
Toshiyuki Takano, and Andria Agusta ............................................................................................. 39
SNAP (Single Nucleotide Amplified Polymorphism) Marker Development Based on Eg-Le
(Length) Gene for Early Identification of Low Height Increment in Oil Palm Annisa S.
Salsabila Diny Dinarti Roberdi and Sudarsono ............................................................................... 40
Diversity Of Stands Type At Buffer Zone Karst Citatah Muhamad Yusup Hidayat ................... 41
Phytochemical Content and Antioxidant Activities of Medicinal Plants from North Sumatra
Gunawan Pasaribu and Totok K. Waluyo ........................................................................................ 42
The Comparison of different diatom digestive method using HCl, H2O2 and HCl, KMNO4 in
Telaga Pengilon Dieng Christopher Hardian Widyanta and Tri Retnaningsih Soeprobowati ....... 43
PE Goat Performance in the Breed Stock Area, Banjarmangu District, Banjarnegara Regency,
Central Java Province Tatan Kostaman, Angga Ardhati Rani Hapsari, Singgih Setiawan,
Bess Tiesnamurti, and Anneke Anggraeni ....................................................................................... 44
Screening Phytochemical Compound of Alstonia scholaris R.Br in Different Sites in Indonesia
Zuraida and Sulistiyani.................................................................................................................... 45
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
BSD City – Tangerang, Indonesia – 24th October, 2019
ix
Current Status and Species Diversity of Seagrass on Panjang Island, Banten Yayuk Sugianti
and Mujiyanto .................................................................................................................................. 46
Effect of Coconut Water and Extract of Banana Hump on The Growth of Binahong
(Anredera cordifolia (Ten) Steenis) Accessions from the Lowland Yaya Hasanah, Lisa
Mawarni, Chairani Hanum, Nurhaida............................................................................................. 47
Evaluation of The Potency of Endophytic Fungi Associated with Artemisia annua and
Artemisia vulgaris as Antibacterial and Antioxidant Evana, Praptiwi, Ahmad Fathoni,
Muhammad Ilyas, Shoji Maehara and Andria Agusta ..................................................................... 48
Distribution of Long-Tailed Monkeys (Macaca fascicularis) in Kelimutu National Park
Ridwan Fauzi, Tonny Wuryanto, Endarto, Fendra Suarmadi, and Albertus Tomonob .................... 49
Local Rice Conservation Through the Local Wisdom of Indigenous Culture at Kasepuhan
Cisungsang Banten Province Pepi Nur Susilawati, Zuraida Yursak and Ulima D Amanda ......... 50
In vitro response of patchouli in a medium by addition of Methylobacterium spp filtrate
Mia Kosmiatin and Seli Salma ......................................................................................................... 51
Legislation on genetically modified animals and its application possibility in Indonesia
M. Ikhsan Shiddieqy, Bess Tiesnamurti and Sri Muharsini ............................................................. 52
Optimization of Xylose Production from Sugarcane Trash by Microwave-Maleic Acid
Hydrolysis Euis Hermiati, Maulida Oktaviani, Riksfardini Annisa Ermawar, Raden Permana
Budi Laksana, Lutfi Nia Kholida, Ahmad Thontowi, and Siti Mardiana ......................................... 53
Estimation of Biodiesel Production from Used Cooking Oil of University Canteen to Support
Sustainable Electricity in Universitas Pertamina Ari Rahman, I Wayan Koko Suryawan,
Ariyanti Sarwono, Nurulbaiti Listyendah Zahra, Ziyadatulkhair M. Faruqi ................................... 54
The Effect of Sucrose Concentrations and Shoot Inoculum Density on The Growth of In Vitro
Shoot of Teak (Tectona Grandis L.) ** Witjaksono, Indira Riastiwi, Katarina Utami
Nugraheni dan Gut Windarsih ......................................................................................................... 55
Screening of Antibacterial and Antioxidant Activities of Stingless Bee (Tetragonula laeviceps
Smith) Extracts Collected from Purwodadi Botanical Garden – East Java, Indonesia Oscar
Efendy, Ahmad Fathoni, Praptiwi, Dewi Wulansari, Wibowo Mangunwardoyo, and Andria
Agusta .............................................................................................................................................. 56
Diversity of The Ecletus Parrot (Ecletus roratus) Birds Inferred From Mitochondraial Gene
Sequences Dwi Astuti .................................................................................................................... 57
Distribution of Long-Tailed Monkeys (Macaca fascicularis) in Kelimutu National Park
Ridwan Fauzi, Tonny Wuryanto, Endarto, Fendra Suarmadi, and Albertus Tomonob .................... 58
Classification of Agarwood Product from Riau – Indonesia using Near Infrared Spectroscopy
Danang Sudarwoko Adi, Dwi Ajias Pramasari, Bernadeta Ayu W, Adik Bahanawan, Yusup Amin,
Sukma Surya K, Wahyu Dwianto, Hwang Sung-Wook and Junji Sugiyama .................................... 59
Identification and Fiber Morphology Measurements of Enggano Island Woods Species
Wahyu Dwianto, Danang S. Adi, Teguh Darmawan, Adik Bahanawan, Eka Lestari, Yusup
Amin, Dwi A. Pramasari, Fitria, Ratih Damayanti, Sri Rulliaty, Junji Sugiyama ........................... 60
Optimum Tempertature for Germanation and Seedling Characterization of The Three Millet
Accessions N Setyowati, P Lestari and A H Wawo ......................................................................... 61
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
BSD City – Tangerang, Indonesia – 24th October, 2019
x
Pcr Column Contamination Gave False Positive Result for Cherax Quadricarinatus
Densovirus (Cqdv) In Sf 9 Cell Cultures Dewi Syahidah, Jennifer Elliman, and Leigh Owens .. 62
Economic Valuation of Forest Fruits Utilization in Batak Toba Community Alfonsus H
Harianja, Anisse M. Sinaga, Ferry A Hawari and Ridwan Fauzi .................................................... 63
Probiotic Powder Production for Cattle by Using Response Surface Methodology
Rohmatussolihat, Roni Ridwan, Nurul Fitri Sari, Rusli Fidriyanto, Wulansih Dwi Astuti,
Yantyati Widyastuti........................................................................................................................... 64
The Effects of Clove Oil On Coral, Trachyphyllia Geoffroy, Using Biomarkers; Color Change
of Coral, Density And Mitotic Index of Zooxanthellae Sri Wahyuni Rahim; Khusnul Yaqin;
Hadiratul Kudsiah ........................................................................................................................... 65
Identification and Test of Fungi On Kaliandra (Calliandra calothyrsus) Seed Which Is
Potential As Antagonist Agent Tati Suharti, Wildani Hanifah, Sri Listiyowati, YMM Anita
Nugraheni, Danu ............................................................................................................................. 66
Potency of Indonesian Nutmeg Oil for Combating Halitosis Yanti, Ika Kristiyaningjati, and
Bibiana Widiyati Lay........................................................................................................................ 67
The Prospect of Microorganism in the Silver Nanopaticle Biosynthesis to Enhance Antibiotic
Drug Activity as an Alternative Solution to Combat Resistances Mega Ferdina Warsito .......... 68
Review: Fungal Glycoside Hydrolases of White-rot Fungi for Cellulosic Biofuels Production
Sunardi, Wiwin Tyas Istikowati, Futhoshi Ishiguri, Shinso Yokota ................................................. 69
Comparison of Dissolved Organic Carbon (DOC) and Physicochemical Variables of Peat
Water Among Three Tropical Peat Swamp Forests Siti Sundari ................................................ 70
Agroforestry Practices In The Use of Dry Land In Gunungkidul District, Yogyakarta,
Indonesia Sudomo A. dan Gerhard E. S...................................................................................... 71
Irradiated Foxtail Millet (Setaria italica (L.) P.Beauv) : Agronomic And Physiological
Performance Under Low Light Intensity Titi Juhaeti ................................................................. 72
Morfological Characterization of Local Durian from Banten Province Zuraida Yursak,
Ulima D Amanda, Dewi Widiyastuti, Pepi Nur Susilawati ............................................................... 73
The Diversity Of Garlic Bulbs And Cloves Quantitative Character In Local Garlic Collection
Of East Java AIAT Ratih Sandrakirana, Maureen Chrisye Hadiatry, Baswarsiati ...................... 74
Detection of Adulteration Activities in Edible Bird’s Nest Using NMR Metabolomics Yong
Chin Hong, Syahidah Akmal Muhammad, Fatimatuzzahra Abdul Aziz, Ng Jing Sheng, Fatin
Ilyani Nasir ...................................................................................................................................... 75
Effect of Amphipilic Lignin Derivatives (A-LD) Surfactant Addition on the Fermentation
Process of Sorghum Bagasse Kraft Pulp for Bioethanol Production W Fatriasari, A Karimah,
F Falah, S H Anita .......................................................................................................................... 76
Traditional Beekeeping of Closed Nested Native Honeybees (Apis spp.) From Four Islands of
Indonesia Sih Kahono, Erniwati, Djunijanti Peggie, & Eko Sulistyadi ........................................ 77
Bioplastic from Modified Cassava Starch, Poly Vinyl Alcohol and Citric Acid Firda Aulya
Syamani, Wida Banar Kusumaningrum, Fazhar Akbar and Bernadeta Ayu Widyaningrum ........... 78
Mechanical Properties Investigation of Green Composite from Ramie (Boehmeria nivea (L.)
Gaud) and Epoxy Ismadi, Safira Vitasasti, Sukma Surya Kusumah ............................................ 79
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
BSD City – Tangerang, Indonesia – 24th October, 2019
xi
Bioconversion of artemisinin by endophytic fungi associated Artemisia sp. grown in Indonesia
and Japan Shoji Maehara, Ahmad Fathoni, Muhammad Ilyas and Andria Agusta ..................... 80
Larvicidal Activity of Cerbera odollam against Culex quinquefasciatus (Diptera: Culicidae)
Didi Tarmadi, Sulaeman Yusuf, S. Khoirul Himmi, Maya Ismayati, Ikhsan Guswenrivo, Titik
Kartika, Dita Meisyara ..................................................................................................................... 81
Conservation of Kelulut (stingless bees) in East and North Borneo, Indonesia Syafrizal,
Irawan Wijaya Kusuma, Oshlifin Rucmana Saud, Restina Wiandany, and Harmonis .................... 82
Allelopathy of Invasive Species Air Potato (Dioscorea bulbifera L.) and Its Effect on
Germination of Meranti (Shorea selanica (Lam.) Blume Ade Ayu Oksari, Irvan Fadli Wanda,
Gladys Ayu Paramita Kusumah Wardhani ....................................................................................... 83
Morphological Characteristic of Apple Velvet (Dispyros blancoi) at Germplasms Garden of
LIPI Cibinong Collection Puspita Deswina, Novia Delliasari Aliyya Az Zahra, Etty Hesthiati .... 84
Bee Pollen from Stingless Bee (Heterotrigona itama) as anti Acne* Enos Tangke Arung,
Irawan Wijaya Kusuma, Ayu Mitha Sari, Yadi, Sukemi, Nataniel Tandirogang ............................... 85
"How Fisheries Technology Affect Production?" (Case Study: North Gorontalo Regency)
Tikkyrino Kuriawan and Hakim Miftahul Huda .............................................................................. 86
The Effect of Sucrose Concentrations and Shoot Inoculum Density on The Growth of In Vitro
Shoot of Teak (Tectona Grandis l.) ** Witjaksono, Indira Riastiwi, Katarina Utami
Nugraheni and Gut Windarsih ......................................................................................................... 87
Compression Strength of 9 Years-old Platinum Teak Wood Mohamad Gopar, Dimas
Triwibowo, Fazhar Akbar, Wahyu Dwianto, Akihisa Kitamori and Takuro Mori ............................. 88
Anti-tubercular Activity of Natural Product-derived Compounds (+)-1,1’ Bislunatin and
(+)-2,2’-Epicytoskyrin A. Listiana Oktavia, Vagolu Siva Krishna, Estharla Madhu Rekha,
Ahmad Fathoni, Nikhila Meda, Andria Agusta, Dharmarajan Sriram ............................................ 89
Growth and Development of Nine Accessions of Mutant Grass Plants (Pennisetum purpureum
cv. Taiwan) in the Field of Induction Mutation of Gamma Ray Husni, A, Maftuha, A.A.
Fatmawaty, N. Hermita, and M. Kosmiatin ...................................................................................... 90
Rheological and Thermal Behavior of Polypropylene–Kenaf Pulp Composite at Different
Fiber and Compatibilizing Agent Loading Nanang Masruchin, Wida Banar Kusumaningrum,
Sudarmanto, Sasa Sofyan Munawar, Ismadi, Putri Amanda, Resti Marlina, Lisman
Suryanegara, Subyakto, Bambang Subiyanto1 and Arif Nuryawan ................................................. 91
Concoctions Developed from Correlations of Meliponicultured Honey with Ethnoentomology
in Langkawi, Malaysia Abu Hassan Jalil, Mohd Razif Mamat, Azira Muhamad and Nor Azfa
Johari ............................................................................................................................................... 92
Carbon Dioxide Absorption and Physiological Characteristics of Selected Tropical Lowland Tree
Species for Revegetation La Ode Muhammad Muchdar Davis, Nuril Hidayati……… ……… … 93
Important Date ............................................................................................................................... 94
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
1
KEYNOTE SPEAKER
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
2
Curriculum Vitae: Dr. Ir. Arifin Rudianto, M. Sc
Deputy of Maritime Affairs and Natural Resources Office Tel : (021) 391 6340
Ministry of National Development Planning Office Fax : (021) 314 4131
Jakarta Indonesia Email : [email protected]
Japan 611-0011
EDUCATION
Doctor of Maritime Policy, University of Wollongong Australia.
Master of Marine Science and Coastal Management, University of Newcastle Upon Tyne,
UK.
Bachelor in Agriculture, IPB University.
WORK EXPERIENCES
2003 - 2005 Director of Sectoral and District Affairs
2005 - 2009 Director of Regional
2009 - 2014 Director of Regional Development
2014 - 2015 Assistant of Minister for Spatial Planning and Maritime
2015 Assistant of Minister for Regional and Distribution
2015 - 2017 Deputy of Regional Development
2017 - now Deputy of Maritime Affairs and Natural Resources
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
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Curriculum vitae : Noriaki Sakaguchi
Senior Advisor, Global Environment Department Office Tel : +81-3-5226-3394
Japan International Cooperation Agency Office Fax : +81-3-5226-6343
Nibancho Center Building, 5-25 Niban-cho,Chiyoda-ku, Email:[email protected]
Tokyo, 102-8012, Japan
FIELD OF RESEARCH
Animal ecology, Biodiversity conservation, Ecosystem management, International policies on
biodiversity
EDUCATION
1989 – 1994 Doctor of Science, Faculty of Science, Kyushu University
Received Ph.D. in Science, March 1994
Title: Social system and ecology of the Iriomote cat Felis iriomotensis
1986 – 1989 Master of Science, Faculty of Science, University of the Ryukyus
Received Master of Science, September 1989
Title: Ecological study of the Iriomote cat Felis iriomotensis
1981 – 1985 Bachelor in Science, Department of Biology, Faculty of Science, University
of the Ryukyus
PUBLICATION 1. Sakaguchi N., 1991, Habitat use of the Iriomote cat and changes in its response to prey
availability, In: Wildlife Conservation (ed. Maruyama, N., Bobek, B., Ono, Y.,
Regelin, W., and Ratcliffe, P.) pp. 137 – 140, Japan Wildlife Research Center, Tokyo.
2. Sakaguchi N., 1994, Ecological aspects and social system of the Iriomote cat Felis
iriomotensis (Carnivora; Felidae), Ph. D. Thesis, 67pp. Kyushu University.
Sakaguchi N. and Ono, Y., 1994, Seasonal change in food habits of the Iriomote cat,
Felis iriomotensis, Ecological Research 9 (2): 167 – 174.
3. Sakaguchi N., Yoneda, M., Sugardjito, J., Effendi, R., Ladjar, N. L. and Suryaningrum,
R., 1997, Preliminary survey of carnivores in Cikaniki area of Gunung Halimun
National Park, Research and Conservation of Biodiversity in Indonesia, Vol. II:
63-80.
4. Sakaguchi N., Yoneda, M., Ladjar, N. L., Sinaga, M. H., 1999, Research and
conservation of the leopard Panthera pardus in Gunung Halimun National Park, West
Java, Indonesia, P: 79 – 83, In Laporan Penyelengaram Ekspose & Lokakarya
Potensi Taman Nasional Gunung Halimun Dan Pemanfaatanya Secara
Berkelanlutan, Bandung, 26-27 Maret, 1999.
5. Syahrial A. H., S. Wijamukti, Koestoto, Sinaga, M. H., Sakaguchi, N., and Okayama,
T., 2001, Leopard (Panthera pardus) monitoring around Cikaniki area in Gunung
Halimun National Park, P.: 39 – 40, In: Simposium dan Seminar Pengelalaan
KeanekaragamanHayati Taman
Izawa, M., Sakaguchi, N. and Doi, T., 2001, The recent conservation program for the
Iriomote cat, Felis iriomotensis, Tropics 10: 79 – 86.
6.
7.
Sakaguchi N., (ed.), 2003, Research on Endangered Species in Gunung Halimun
National Park, Research and Conservation of Biodiversity in Indonesia, Vol. XI,
70pp. Bogor.
Sakaguchi N., Kuswandono, Ekawati, D., Syahrial A. H., and Mulyati, S. (ed.), 2003,
The action plan for the conservation of endangered species in Gunung Halimun
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
4
8.
National Park and its surrounding area, 36pp. Kabandungan.
Syahrial A. H. and N. Sakaguchi, 2003, Monitoring Research on the Javan Leopard
Panthera pardus melas in a Tropical Forest, Gunung Halimun National Park, West
Java, Research and Conservation of Biodiversity in Indonesia, Volume XI: 2-28.
9. Sakaguchi N., Coastal Marine Ecosystem and Blue Carbon, an International
Cooperation for their Conservation in Coral Triangle Area, International Cooperation
of Agriculture and Forestry, Vol.41(2): 2-14, In Japanese
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
5
IPBES Assessments on Bioodiversity and Ecosystem serveces and Toword
Post 2020 Framework and Achievement of SDGs
Noriaki Sakaguchi
Fosret and Nature Conservation Group, Global Environment Department, Japan International
Cooperation Agency,
Nibancho Center Building, 5-25 Niban-cho, Chiyoda-ku, Tokyo 102-8012, Japan
*Corresponding author: [email protected]
Abstract. IPBES published its regional and global assessment reports on biodiversity and
ecosystem services in 2018 and 2019, in respectively. Biodiversity and ecosystem
services, providing foods, regulating the climate and reducing natural disaster risks, are
essential for human existence and good quality of life. However, they are deteriorating
worldwide. 75% of the land surface is significantly altered. About 1 million species
among existing fauna and flora face extinction within decades. In Asia and the Pacific,
the rich biodiversity and valuable ecosystem services support 4.5 billion people (64.3%
of the world population), while they are declining in most of the region due to rapid
economic growth. Forest cover in South-East Asia was largely reduced by 12.9% from
1990 to 2015. Direct drivers of the changes are land/sea use change, over exploitation,
climate change, pollution and invasive alien species. Indirect drivers include production
and consumption, human population, trade, technological innovations and governance.
Regarding the Aichi Targets, the progress in extent of protected areas (Target 11) is
significant, but those in most of other targets are insufficient to meet. It‟s also necessary
to mainstream biodiversity in key economic sectors of agriculture, forestry and fisheries,
tourism, energy and mining, infrastructure, manufacturing and processing. Since
compounding effects of direct and indirect drivers exacerbate negative impacts on
biodiversity and ecosystems, the post 2020 biodiversity targets and SDGs can only be
achieved through transformative changes across economic, social, political and
technological factors. Specifically, integrated approaches through cross-sectoral
cooperation is necessary to enable transformative change.
Keywords : IPBES, biodiverswity, ecosystem services, Aichi Targets, SDGs
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
6
INVITED SPEAKER
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
7
Curriculum vitae : Dr. Lin Chun Yi
Research Instituter for Sustainable Humanosphere Office Tel: +81-774-38-3604
Kyoto University
Gokashou, Uji Email: [email protected]
Japan 611-0011
FIELD OF RESEARCH
My previous research interest was on citrus systemic diseases (citrus huanglongbing, citrus
tristeza, citrus tatter leaf and citrus exocortis) and on understanding the basic characterization
and disease ecology of viroids and how two viroids interact with each other in citrus host. My
recent research topic focuses on the impact of virus spillover from invasive species to native
pollinator community. in the project, I investigate the prevalence and distribution of honeybee
viruses in two global invasive ant species. The interaction between honeybee, virus and
invasive ant can be a standard example for understanding the pathogen transmission route
from native to invasive species.
EDUCATION
2010 – 2015 Ph.D., Department of Plant Pathology and Microbiology, National Taiwan
University
2004 – 2008 B.S., Department of Plant Pathology and Microbiology, National Taiwan
University
PUBLICATION 1. Lin, C. Y., Chen, Y. H., Wu, M. L., Su, H. J., and Hung, T. H. (2018). Pathological
categorization of the stem-pitting and mild strains Citrus tristeza virus in Taiwan and
their genomic analysis. J Phytopathol 166, 459-469.
2. Lin, C. Y., Chang, L., Lin, Y. H., Cheng, H. J., Wu, M. L., and Hung, T. H. (2018).
Biological and molecular characterization of citrus tatter leaf virus in Taiwan. Plant
Pathol 67, 995-1008.
3. Lin, C.Y., Tsai, C. H., Tien, H. J., Wu, M. L., Su, H. J., and Hung, T. H. (2017).
Quantification and ecological study of Candidatus Liberibacter asiaticus in citrus
hosts, rootstocks and the Asian citrus psyllid. Plant Pathol 66, 1555-1568.
4. Lin, C. Y., Wu, M. L., Shen, T. L., Yeh, H. H., and Hung, T. H. (2015). Multiplex
detection, distribution, and genetic diversity of Hop stunt viroid and Citrus exocortis
viroid infecting citrus in Taiwan. Virol J 12, 11.
5. Lin, C. Y., Wu, M. L., Shen, T. L., Yeh, H. H., and Hung, T. H. (2015). Multiplex
detection of Citrus viroids: advances and future application. Br J Virol 2(1), 12-14.
6. Lin, C. Y., Wu, M. L., Shen, T. L., and Hung, T. H. (2015). A mutual titer-enhancing
relationship and similar localization patterns between Citrus exocortis viroid and Hop
stunt viroid co-infecting two citrus cultivars. Virol J 12, 142.
7. Lin, C. Y., Wu, M. L., and Hung, T. H. (2015). Identification of two commercial
tomato cultivars as new indicator plants for detecting Citrus exocortis viroid infection.
Plant Pathol Bull 24(1), 01-11.
8. Wu, M. L., Shen, T. L., Chang, T. T., Chen, C. H., Lin, C. Y., Lee, C. Y., and Hung, T.
H. (2015). Establishment of a rapid PCR detection method for „„Antrodia salmonea‟‟
and „‟A. cinnamomea‟‟. Taiwan J For Sci 30(1), 45-53.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
8
Emerging threats of invasive ants to native pollinator community: insights
into virus spillover
Chun-Yi Lin and Chin-Cheng Yang*
Research Institute for Sustainable Humanosphere, Kyoto University
Gokasho, Uji, Kyoto 611-0011, Japan
*Corresponding author: [email protected]
Abstract. Emerging virus diseases consistently threaten pollinator species worldwide.
Recent studies have shown that ants often are infected by honeybee viral pathogens likely
during their interactions with honeybee, suggesting that cross-species transmission of
honeybee viruses may have occurred commonly than previously thought. However, these
studies are either at regional level or focused on only one or two honeybee viruses. We
describe six honeybee viruses in two most widespread and damaging invasive ants (Yellow
crazy ant (Anoplolepis gracilipes) and Longhorn crazy ant (Paratrechina longicornis) in
Asian regions. Depended on the ability of fast reproduction and well-adaptation to new
environment, virus spillover could occur from invasive ants, vector for virus reservoirs, to
local honeybee populations. Foreign lethal viral species may be introduced to pollinator
community via cross-species interaction and have severe impacts to native ecosystem. The
Large-scale survey of honeybee viruses in invasive ants provide insight to break point in
pathogen prevention and also offer new information for possible transmission routes
between invasive and pollinator species.
Keywords : Anoplolepsis gracilipes, Paratrechina longicornis, virus spillover, honeybee
virus
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
9
Curriculum vitae : Prof. Subyakto
Research Center for Biomaterials, Indonesian- Office Tel : +62-21-87914511
Institute of Sciences Office Fax: +62-21-87914510
Jl. Raya Bogor Km 46, Cibinong, Bogor 16911 Email:[email protected]
Indonesia
FIELD OF RESEARCH
Biocomposites
EDUCATION
1999 – 2002 Doctor of Agriculture, Kyoto University, Japan
.
1987 – 1990 Master of Forest Science, The University of Melbourne, Australia
.
1978 – 1982 Bachelor in Forestry, Bogor Agriculture University, Indonesia
PUBLICATION 1. Budiman, I., Hermawan, D., Febrianto, F., Subyakto, Pari, G. 2019. Char properties
and pollutant adsorption capability of oil palm shell using hydrothermal process.
Biomass Conversion and Biorefinery. doi: 10.1007/s13399-019-00394-5
2. Endrawati, Y.C., Solihin, D.D., Suryani, A. Subyakto. 2019. Improving Poly (Lactic
acid) Properties by Using Fibre Reinforcement of Wild Silkworm Attacus atlas.
Pakistan J. Sci. Ind. Res. Ser. A: Phys. Sci. 62A (1): 38-47
3. Budiman, I., Hermawan, D., Febrianto, F., Subyakto, Pari, G. 2019. Optimal activation
of activated char from oil palm fruit shell using response surface methodology. Journal
of Tropical Wood Science and Technology. 17(1): 8-21
4. Prasetiyo, K.W., Octaviana, L., Astari, L., Syamani, F.A., Subyakto, Achmadi, S.S.
2018. Physical-Mechanical Properties and Bonding Mechanism of Corn Stalks
Particleboard with Citric Acid Adhesive. Journal of Tropical Wood Science and
Technology. 16(2):132-141
5. Suryanegara, L., Yuliansih, P., Syamani, FA., Laksana, RPB., Subyakto, Abidin, Z.,
Masruchin, N., Indrayani, M. 2018. Effect of Phenylphosphonate Acid – Zinc, talk and
Triacetine on crystalline speed of polylactid acid. J. Sains Materi Indonesia.
19(3):93-97
6. Yuni Cahya Endrawati, Dedy Duryadi Solihin, Ani Suryani, Subyakto. 2017.
Optimization of Fibroin Yield of Bombyx mori L. dan Attacus atlas L. using Response
Surface Methodology. Agritech Jurnal Teknologi Pertanian. 37 (2): 205-214
7. Wida Banar Kusumaningrum, Rochmadi, Subyakto. 2017. Preparation of Acetylated
Cellulose from Pulp of Betung Bamboo (Dendrocalamus asper) and its effects on
mechanical properties of polypropylene composites. Reaktor 17 (1): 25-35
8. Dungani, R., Karina, M., Subyakto, Sulaeman, A., Hermawan, D., Hadiyane, A. 2016.
Agricultural Waste Fibers Towards Sustainability and Advanced Utilization: A Review.
Asian Journal of Plant Sciences 15(1-2):42-55
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
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9. Pramono, A.E., Rebet, I., Zulfia, A., Subyakto. 2015. Tensile and shear punch
properties of bamboo fibers reinforced polymer composites. International Journal of
Composite Materials 5(1):9-17
10. Syamani, F.A., Subyakto, Sukardi, Suryani, A. 2015. Changes in oil palm frond fiber
morphology, cellulose crystallinity and chemical functional groups during cellulose
extraction phases. Chemistry and Materials Research 7(3):105-113
11. Ismadi, Yuwono, A.H., Astutiningsih, S., Subyakto. 2015. Effect of Carbon Fiber
Loading in Mechanical Properties and Electrical Conductivity of Polyvinyl Alcohol
Based Composites. Macromolecular Symposia 353(1):102–107
12. Nugroho, A., Satyarno, I., Subyakto. 2015. Bacteria as Self-Healing Agent in Mortar
Cracks. J. Eng. Technol. Sci. 47 (3): 279-295
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
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Biocomposites from Natural Fibers
Subyakto*, Nanang Masruchin and Sukma Surya Kusumah
Research Center for Biomaterials, Indonesian Institute of Sciences,
Jl. Raya Bogor Km 46, Cibinong-Bogor 16911, Indonesia
*Corresponding author: [email protected]
Abstract. Indonesia has big potential of natural fibers from many sources of raw
materials such as original natural fibers, wastes of agriculture, estates, and forestry
activities. Waste from oil palm industry is the biggest source of fiber materials, for
example empty fruit bunches, trunks, and fronds. The natural fibers and wastes are not
optimally utilized; therefore they are potential to be used as raw materials of composites
industry to make added values. In comparison with petroleum based synthetic fibers,
natural fibers have some advantages; such as lightweight, low cost, sustainable,
recyclable, and biodegradable. In addition they have good thermal and acoustic properties,
machine friendly (less abrasive), and easier to process. Many composites materials from
natural fibers had been developed in Indonesia mainly in the laboratory scale.
Particleboard, medium density fiberboard, laminated board, fiber-cement board,
fiber-plastic composite, and molding product have been developed. Composites from
bamboo, corn stalk, kenaf, pineapple, ramie, sugarcane bagasse, sorghum bagasse, empty
fruit bunches, trunks, and fronds have been subjected to many research in Indonesia.
Adhesive is very important factor in manufacturing of natural fiber composites;
preferably it is formaldehyde free, low cost, and has excellent performances. Citric acid
or combination of citric acid and sucrose had been developed as environmentally friendly
adhesive for particleboards. Natural fiber composites have many applications such as
building components, furniture, automotive parts, medical applications, packaging, and
sport goods. Utilization of natural fibers will be more and more significant in the future.
The future trends of utilizations of natural fibers among them are bio-composites,
nano-fiber-composites, and advanced materials such as supercapacitors.
Keywords : biocomposites; natural fibers; utilization, Indonesia
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
12
Curriculum vitae : Dr. Elpidio V. Peria
ASEAN CENTRE FOR BIODIVERSITY Office Tel : +6349-536-2865
Domingo M. Lantican Avenue, College, Office Fax: +6349-584-4210
Los Baños, Laguna 4031 Philippines Email: [email protected]
FIELD OF WORK
Biodiversity policy formulation, development and advocacy– international, national, local –
SEAsia and Philippines
EDUCATION
1991-1995 Bachelor of Laws, University of the Philippines, Diliman, Quezon City
1983-1987 Bachelor of Science in Fisheries, major in Fisheries Business Management,
University of the Philippines, Diliman, Quezon City
EXPERIENCE
Years
1997-present
Over 2O years of litigation experience, including policy advocacy and
formulation; treaty negotiations in Nagoya Protocol and art. 10 of Paris
Agreement on Technology Development and Transfer
Regular blog writer since 2012
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
13
Balancing economic development and biodiversity protection – Biodiversity
value chains & certification of products
Peria, Elpidio1 ; Schlegel, Bianca2 ; Dr. Simorangkir, Dicky3
1 ASEAN Centre for Biodiversity (ACB); Los Baños, Laguna, Philippines *
2 GFA Consulting Group, Hamburg, Germany
3 Deutsche Gesellschaft für International Zusammenarbeit (GIZ) GmbH, Eschborn, Germany
Abstract. ASEAN´s wealth of biological resources poses immense opportunities for
economic development. The ASEAN Member States want to use this economic potential
by further developing sustainable trade relations for bio-products nationally, regionally and
possibly globally. However, the exact market potential of Biodiversity-based Products
(BBP) is often still unknown. The “Biodiversity-based Products (BBP) as an Economic
Source for the improvement of Liveli-hoods and Biodiversity Protection” Project, funded
by the German government via the Deutsche Gesellschaft für International
Zusammenarbeit (GIZ) GmbH, is implemented in cooperation with the ASEAN Centre for
Biodiversity (ACB) by GFA Consulting Group, to pilot biodiversity-based value chains in
Cambodia, Laos and Vietnam, promoting conservation through sustainable use. Villagers
around protected areas, local park management, cooperatives and private sector business
partners cooperate for the development of marketable products where they did not yet exist
or for upgrading of existing products. Biodiversity value chains starts with sustainable
sourcing of raw material, including management plans for the input resources which also
comprises in-situ and ex-situ planting to enhance available raw material. Value is added
through enhanced processing steps and especially certification (e.g. ODOP certification for
bamboo) which improves market access for the villagers and their products.
Keywords : biodiversity value chains; biodiversity-based products; biological resources;
certification
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
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Curriculum vitae : Ms Margarita Naming
Sarawak Biodiversity Centre Tel: 00 6 082 610610
KM20, Jalan Borneo Heights Fax: 00 6 082 611535
Semengoh Email: [email protected]
Locked Bag No. 3032
93990 Kuching Sarawak
Malaysia
FIELD OF RESEARCH
leading the Traditional Knowledge Documentation programme at the Sarawak
Biodiversity Centre (SBC)
spearheaded the development and implementation of Access (Prior Informed Consent)
and Benefit Sharing with various ethnic communities in Sarawak based on the State‟s
biodiversity law on Access and Benefit Sharing (ABS)
leads a team in developing and implementing the Sarawak Biodiversity Centre Ordinance
1997 and Sarawak Biodiversity Regulations 2016.
EDUCATION
2015 Masters in Environmental Sciences from Universiti Malaysia Sarawak
(UNIMAS)
1997 Degree in Zoology from the National University of Malaysia (UKM)
PUBLICATIONS
“Building a discovery partnership with sarawak biodiversity centre: a gateway to access
natural products from the rainforests”
Authors: Chia Yeo, Tiong; Naming, Margarita; Manurung, Rita
Source: Combinatorial Chemistry & High Throughput Screening, Volume 17, Number 3,
2014, pp. 192-200(9)
“Maximizing Biodiversity Potential Through Traditional Knowledge documentation: A
Study Case”
Authors: Jern Ern Chuah, Rita Manurung, Margarita Naming
Source: Journal of Intellectual Property Law & Practice, Volume 9, Issue 5, May 2014,
Pages 397–402, https://doi.org/10.1093/jiplp/jpu041
Speaker in International Workshops relating to ABS
(i) 2019 Southeast Asia Symposium of Traditional Medicine: Experience Sharing &
Future Cooperation, 13 - 14 July 2019, National Yang-Ming University School of
Medicine, Taiwan,
(ii) ASEAN Conference on Biodiversity 2016, 15-19 February 2016, Bangkok, Thailand
(iii) Bhutan National Seminar on Traditional Knowledge Associated with Biological
Resources, 29 - 30 March 2016, Bhutan
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
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(iv) Regional Workshop on Access to Genetic Resources and the Fair and Equitable Sharing
of Benefits Arising from their Utilization (ABS): Understanding the Nagoya Protocol,
25-26 October 2011, Manila
(v) Southeast Asia Training-Workshop on Building Institutional and Stakeholders
Capacities on Access to Genetic Resources and the Fair and Equitable Sharing of
Benefits Arising from their Utilization (ABS), 20 to 23 August 2012, The Imperial
Queen‟s Park Hotel, Bangkok, Thailand
Participation in International Meetings on ABS
Ninth Conference of Parties to the Convention on Biological Diversity (COP-9) from 19
to 30 May in Bonn, Germany
7th Ad-Hoc Working Group on Access and Benefit Sharing of Biological & Genetic
Resources (ABS) in Paris from 2 to 8 April 2009
Resumed Meeting on Working Group of Access and Benefit Sharing (16-17 October
2010) and the Tenth Conference of the Parties to the Convention on Biological
Diversity (CBD COP 10) from 18 to 29 October 2010 in Nagoya, Japan. During the
COP 10 meeting, the Nagoya Protocol on ABS was adopted by the Parties.
12th Conference of Parties to the Convention Biological Diversity (COP-12), Pyeong
Chang, Korea.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
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Successful Experience and Gaps in Access and Benefit Sharing (ABS)
through Research on Traditional Knowledge in Sarawak, Malaysia
Margarita Naming* and Yeo Tiong Chia
Sarawak Biodiversity Centre KM20, Jalan Borneo Heights, Semengoh, Locked Bag No. 3032,
93990 Kuching Sarawak
Malaysia
*Corresponding author: [email protected]
Abstract. After more than two decades since 1992, the third objective of the Convention
on Biological Diversity (CBD), is recognized with the coming into force of the Nagoya
Protocol on Access to Genetic Resources and the Fair and Equitable Sharing of Benefits
Arising from their Utilization in 2014. This Protocol “only” provides the core obligations
framework for contracting parties in relation to access, benefit-sharing and compliance
which is the “ABC” of the Nagoya Protocol. In Sarawak, the CBD objectives were
applied through the Sarawak Biodiversity Centre Ordinance in 1997. The Ordinance
provides clear and current provisions on traditional knowledge (TK) documentation and
bioprospecting and subsequently provisions on access and benefit sharing (ABS) in line
with the Nagoya Protocol. This paper shares the beginning to an end of a successful case
in the implementation of ABS which include Prior Informed Consent (PIC) and sharing of
benefits; an effort that took fourteen (14) years. The pilot projects on TK and ABS clearly
shows that feasibility as well as the value can be derived. The communities need an
establishment of trust to agree to such a project, and are motivated by continuous
engagement and empowerment. The projects also identify various challenges in
determining royalties and margins, managing third party involvement, multiple
communities with a similar genetic resource, value disruption, supplies of raw materials,
sustainability, scale-up, disbursement of monetary benefits and community participation.
Keywords : Access and Benefit Sharing, Prior Informed Consent, Sarawak Biodiversity
Centre, traditional knowledge documentation, bioprospecting
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
17
Curriculum vitae : Mr. Erlambang Ajidarma
Erlambang Ajidarma is head of product innovation at Mycotech. He received BSc. in
Microbiology (2012) at Institut Teknologi Bandung. He has been work at Mycotech since
2016 developing biomaterial from waste agriculture and work together with ETH Zurich and
Future Cities Laboratory - National University of Singapore in Mycotree development for
Seoul Biennale 2017. His focus research right now in Mycotech is developing mushroom
composite board and leather-like material from agricultural waste.
EXPERIENCE
1. January 2017 to Current : Miko Bahtera Nusantara Bandung (Mycotech), Indonesia
Head of Product Innovation
Development of biomaterial as structure material from agricultural waste using
mushroom as binder (Future Cities Laboratory – Singapore-ETH Zurich)
Mushroom waste as oil adsorbent (Pertamina)
Mushroom waste as biobricket (Pertamina)
Substrate effect to fungal composite materials
Growth optimizing of fungal polypore in producing mycelial sheet
2. April 2013 to December 2016 : Charoen Pokphand Indonesia Jakarta, Indonesia
Research Assistant
Isolation and assessment of lactic acid bacteria from free range chicken as poultry
probiotic
Growth optimizing of photosynthetic bacteria in batch reactor system
Assessment of photosynthetic bacteria as H2S reducing agent in aquaculture pond
EDUCATION
2012 Institut Teknologi Bandung Bandung – Bachelor of Science Microbiology
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
18
Mycelium-Composite as Future Biomaterial
Erlambang Ajidarma
Mycology Researcher
Mycotech
Abstract. Indonesia as an agricultural country has abundant crop and plantation products.
Based on data from Indonesia‟s Ministry of Agriculture, in 2017, Indonesia has
productive plantations of 25 million hectares with production value up to 46 million tons
for palm oil, coconut, rubber, coffee, and cocoa. This huge production value generates
agricultural wastes up to 14 million tons of biomass. Fungal, with its ability to digest
cellulose and lignin, can play an important role in utilizing the agricultural wastes.
Mycelium, root structure of fungal, acts as natural glue that can bind organic materials
together and transform it into natural biocomposite called mycelium-composite. By using
fungal as a binding agent, agricultural wastes like baggase, sawdust, and woodchip can be
converted into mycelium-composite that has flame retardant and good insulation
properties. Furthermore, providing physical treatment such as hot-pressed system to
mycelium-composite can alter the composite into binderless board with strength
equivalent to particle board. Although mycelium-composite is a promising biotechnology
product, its development as a biomaterial is still early considering there are many aspects
that can be explored, such as combination of agricultural wastes, type of fungus used, as
well as types of fungus growing methods.
Keyword : agricultural waste, fungal, natural glue, mycelium composite
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
19
ORAL PRESENTATION
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
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Honey, a persisting NTFP on a deforested peat landscape of eastern
Sumatra
Ahmad Muhammad 1* Abdul Ronny
2 and Yasuyuki Kono
3
1Dept. Biology, Fac. Math. & Natural Sciences, Riau University, Pekanbaru, Indonesia
2Center for Southeast Asian Studies (CSEAS), Kyoto University, Kyoto, Japan
*Corresponding author / email: [email protected]
Abstract. The peat landscapes of eastern Sumatra have undergone severe deforestation,
which has deprived the areas of valuable forest products, such as timber and various
non-timber forest products (NTFPs). The present study focused on the honey of Apis
dorsata, which is one of the most important NTFPs. Despite the loss of natural forests, we
recently observed honey gathering activities on Bukit Batu peat landscape in Bengkalis
Regency, Riau Province. The giant honeybee adaptively responds the loss of large and tall
trees by building solitary nests in smaller trees in non-forest vegetations. We conducted
both explorative and plot-based systematic surveys at three villages to assess the nest
distribution and density. A total of 146 nests were detected in young secondary forests
(38.4%), rubber plantations (37.0%), oil palm plantations (21.9%), and home gardens
(2.7%). The average nest density in such vegetation mosaic systems was 0.23 nests/ha.
During „honey seasons‟ the average number of nests cut in each village was 26.6
nests/month and the average amount of honey collected was 137.6 kg/ month, which
potentially fetched a collective income of about 11 million IDR/month. The findings
suggest that production of forest honey in peatland areas may persist despite the loss of
natural forests.
Keywords : Apis dorsata; Bukit Batu; honey gathering; peatland area; vegetation
mosaic
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
21
A Preliminary Study on The Population and Habitat of Saltwater Crocodile
(Crocodylus porosus) in Timor Island, East Nusa Tenggara
Grace Serepina Saragih*1,2
, Kayat2, Muhammad Hidayatullah
3,2 , Dani Sulistyo Hadi
2
1Research and Development Center for Environmental Quality and Laboratory
Gd. 210, Komplek Puspiptek, Serpong, South Tangerang, Indonesia 2Kupang
Research and Development Institute for Environment and Forestry
Jl. Alfons Nisnoni No.7, Kupang, East Nusa Tenggara, Indonesia 3Research and Development Institute of Technology Non Timber Forest Product
Jl. Dharma Bakti No.7, Lombok Barat, West Nusa Tenggara, Indonesia
*Corresponding author : [email protected]
Abstract. The information on the population size and habitat condition of Saltwater
crocodile in East Nusa Tenggara is unavailable. The present paper discusses preliminary
data on population and habitat characteristic of the Saltwater crocodile in three
conservation areas in Timor island, namely Teluk Kupang Marine Nature Park, Menipo
Nature Park, and Maubesi Mangrove Forest Nature Preserve. Spotlight surveys and
vegetation analysis were carried out in each location. Crocodile encounter rate was
calculated as individuals detected per km river surveyed. The encounter rate in Teluk
Kupang, Menipo and Maubesi was 0.3 crocodiles/Km, 0.4 crocodiles/Km, and 0.6
crocodiles/Km respectively. Hatchlings, juveniles and adult saltwater crocodile were found
in in Menipo and Maubesi but only adults were found in Teluk Kupang. Crocodiles prefer
less saline waters and sheltered areas such as mangrove roots. Mangrove density in
Teluk Kupang, Menipo and Maubesi was 127 trees/ha, 124 trees/ha and 186 trees/ha
respectively. These were the first systematic surveys in the areas studied and additional
work is needed to further characterize the population and habitat of saltwater crocodile in
East Nusa Tenggara.
Keywords : habitat, population, saltwater crocodile, Timor
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
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22
High Corolla Colour Variation of Hoya coronaria in Belitung Island:
Potential Use and Conservation
Sri Rahayu1)
* & Yulian Fakhurrozi2)
1) Research Center for Plant Conservation and Botanic Gardens- Bogor Botanic Gardens,
Indonesian Institut of Sciences (LIPI), Jl. Ir. H. Juanda 13 Bogor- 16122 2)
Badan Pengelola Geopark Pulau Belitung – sektretariat Belitung Timur, Kabupaten Belitung
Timur, Kepulauan Bangka Belitung 33512
*contact person, email: [email protected]; [email protected]
Abstract.The genus Hoya (Apocynaceae: Asclepiadadoideae) has become popular as
ornamental plant due to the beauty of its shiny, waxy, star shaped corolla with various
colour. Normally, each species has specific corolla and corona colour. But some species
has variation in corolla colour, for example Hoya coronaria Blume. Normally, H.
coronaria has white with pinkish dotted corolla colour, rarely with red corolla colour. H.
coronaria distributed from South Thailand, Malay Peninsula, Java, Borneo and Sumatra
as well as in Belitung Island. We surveyed the population and corolla colour of H.
coronaria in Belitung Island in June. The RHS colour chart was used to determine the
colour. We found 8 accession with differen corolla and corona colour variation of H
coronaria in Belitung Island, ranging from white, cream, pink, peach, deep pink, yellow,
red, and deep red. This variation was very high and population of Hoya coronaria well
developped in the heath forest habitat of Belitung Island. The further utilization was
suggested as domesticated ornamental plant with proper characterization to support
further breeding program. H coronaria was also a component of heath forest habitat of
Belitung Island, wich is very fragile, thus the ex situ and in situ conservation was
suggested for sustainibility.
Key words: bioresource, flower, germplasm, Heath forest, Hoya coronaria, variavility
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
23
Exploration of Indonesian Nutmeg Seed and Its Active Compound for
Management of Arthritis Inflammation
Yanti*, Yustine, Marlin Elza, and Hans Christian Leman
Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta 12930,
Indonesia
*Corresponding author: [email protected]
Abstract. Gout or arthritis inflammation is caused by the accumulation of monosodium
urate in various connective and joint tissues that lead to the formation of uric acid. The
presence of uric acid induces local inflammation and neutrophile production by
over-expression of pro-inflammatory cytokines, including tumor necrosis factor (TNF)-α,
inducible nitric oxide synthase (iNOS), and interleukin (IL)-6. The use of chemical drugs
for gout treatment causes side effects, like liver and kidney failure, and hypersensitivity.
Thus, finding natural theurapeutics from plants has been the focus of investigation. Here,
we investigated whether nutmeg extract (Myristica fragrans) and its active compound,
macelignan, inhibited the expression of pro-inflammatory cytokines in
lipopolysaccharide (LPS)-induced chondrocytes in vitro by conducting MTT,
SDS-PAGE, and ELISA analysis. MTT profile showed that nutmeg extract and
macelignan up to 10 µg/ml only had less toxicity on cell viability and cell morphology.
SDS-PAGE profile demonstrated that chondrocytes treated with LPS secreted TNF-α
protein with molecular weight of 51 kDa. ELISA data showed that treatment of nutmeg
extract and macelignan dose-dependently inhibited TNF-α, iNOS, and IL-6 expression in
LPS-induced chondrocytes, as well as ibuprofen standard. In conclusion, nutmeg extract
and its macelignan may provide the natural anti-gout arthritis potency for management of
arthritis inflammation.
Keywords: arthritis inflammation, chondrocytes, macelignan, nutmeg, anti-gout effect
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
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24
Health Percentage Of Coral Reef In Tunda Island, Banten Province
Mujiyanto*) and Joni Haryadi
*) Research Institute for Fisheries Enhancement
Cilalawi Street No. 1, Jatiluhur Purwakarta, West Java Indonesia – 41152
*Corresponding author : [email protected]
Abstract. Coral reef ecosystem is once important ecosystem to support people life in
coastal areas. Once of community has been dependencing coastal areas especially coral
reef ecosystem is community in Tunda Island, it was because around 80 % people in
Tunda Islands were dependencing their livelihood in coral reef areas. The degradation of
coral reef habitat was in Tunda Island to give out significant effect for people life in
Tunda Island. The benefits that is coral reef ecosystem potency, being the people has
been dependency from resources. This study aimed to now condition habitats and
analysis of coral reef ecosystem potency. It was conducted in July 2018, July 2019 and
September 2019. The method survey was underwater photography transect method at 2
depths areas were ± 3-4 m and ± 10-11 m. Result analysis of coral reef percentage cover
reef cover were in 8 categories group base from Coral Point Count with Excel extensions
(CPCe) seri 4.1. output. Values of percentage coral reef cover in first depth (± 3-4 m) has
found percent cover of health reef that is grouping in category Coral (C) on each side of
Tunda Island are 75,39 % (east side‟s island), 51,81 % (north side‟s island), 37,90 %
(west side‟s island) and 66,42 % (south side‟s island). The percentage of coral reef on the
east side was 75.39 % include in very good of health category. The result of heath
category in second depth also was founded in east side‟s island with the value was 58,87
% (good category). Different of percentage covers value was suspected more affects from
people activities for did their activities in coastal areas especially in coral reef ecosystem.
Keywords: present cover, habitats, coral reef, tunda island
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
25
Development of Bamboo Propagation for Source Material of Bamboo-based
Activated Carbon
Riksfardini A. Ermawar1*
, Widya Fatriasari1, Rikno Harmoko
2, N. Sri Hartati
2, A. Dany
Sunandar3, Ratih Damayanti
3, Saptadi Darmawan
3
1Research Center for Biomaterials, Indonesian Institute of Sciences,
2Research Center for Biotechnology, Indonesian Institute of Sciences,
1,2Cibinong Science Center, Cibinong-Bogor 16911, Indonesia
3Forest Product Research and Development Center, Ministry of Environment and Forestry
(KLHK), 3Jl. Gunung Batu No. 5 - Bogor 16610, Indonesia
*Corresponding author: [email protected]
Abstract. Bamboo species are widespread in many regions in Indonesia. They are
becoming an important part of Indonesian cultural and social life. They have been
vastly-used and have economic value as renewable resources. They can be made into
various bio-products, including bamboo-based activated carbon. Based on preliminary
study of the characterisation of bamboo-based activated carbon from six different
bamboo species, we chose to develop few of them by both ex vitro and in vitro
propagations. In this study, bamboos were collected from private and government
nurseries. In the ex vitro propagation, induction of shoots was trialled to five different
bamboo species. The growth of shoot cuttings was observed in the media of husk
charcoal - coco peat. Meanwhile, initiation of the in vitro propagation was trialled to
three bamboo species, namely Gigantochloa robusta, Dendrocalamus asper and
Oxythenantera abbissinca. Two sterilisation methods were applied to sterile two types of
explant (i.e. young stem and shoot). Young shoots from sterile explants will then be
transferred into various combinations of induction medium for callus initiation, including
2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA),
indole-3-butyric acid (IBA) and cytokinins (kinetin). This research opens a possibility of
generating future bamboo with improved characteristic, in this case generating bamboo
with higher density of lignocellulose content.
Keywords : activated-carbon, callus, explant, ex vitro, in vitro, tissue culture
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
26
Wood Quality of Kelempayan and Benuang from East Kalimantan,
Indonesia: Fiber Quality and Juvenile-Mature Boundary
Sari Delviana Marbun1, Imam Wahyudi
2*, Jajang Suryana
2, Deded Sarip Nawawi
2
1Science and Forest Products Technology Study Program, IPB University, Bogor, 16680,
Indonesia 2Department of Forest Products, Faculty of Forestry, IPB University, Bogor, 16680, Indonesia
*Corresponding author: [email protected]
Abstract. Scarcity of wood supply for wood industries in Indonesia is a serious problem.
Lesser-used wood species are potential to be alternative resources to overcome the scarcity
of wood supply. The aims of this research were to evaluate wood quality, mainly radial
variation of fiber quality and juvenile-mature boundary of two lesser-used wood species,
namely kelempayan and benuang. Fiber quality was analysed following Rachman and
Siagian‟s method. Radial variation of fiber and vessel length were used as parameters to
estimate juvenile-mature boundary using polynomial and segmented regression models.
The results showed that benuang fiber is classified as Quality Class I, while kelempayan
fiber is Quality Class II for pulp and paper manufacturing. In kelempayan wood, the
boundary of juvenility occurred in the 6 and 7th segments from the pith, while the whole of
benuang wood was still juvenile. Boundary between juvenile and mature wood on these
wood species was affected by parameter and method applied.
Keywords : benuang, fiber quality, juvenility, kelempayan, tropical rain forest
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
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27
The Effects of FeCl3 Concentration on Hydrothermal Pretreatment of Oil
Palm Fronds to Enhance Reducing Sugar Production
Nurwahdah1, Rodiansono
1, A Kamari
2, R Abdullah
3, Sunardi
1,4*
1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Lambung
Mangkurat University, Banjarbaru 70714, Indonesia 2Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan
Idris, 35900, Tanjung Malim, Perak, Malaysia 3Department of Applied Physics, Faculty of Science, Universiti Brunei Darussalam, BE1410,
Brunei Darussalam 4Wetland-Based Materials Research Group, Lambung Mangkurat University, Banjarbaru
70714, Indonesia
*Corresponding author: [email protected]; [email protected]
Abstract. The effects of FeCl3 concentration on hydrothermal pretreatment of oil palm
fronds (OPF) to enhance reducing sugar production have been systematically investigated
for the first-ever. The hydrothermal pretreatment was carried out in autoclave reactor with
Teflon at 190 oC for 30 min with various FeCl3 concentration of 0.075; 0.150; and 0.225 M.
The residue from hydrothermal pretreatment (the noted as substrate) was hydrolyzed by
enzyme (Cellulase Onozuka RS) in water bath shaker at 50 °C for 48 h. The amount of
reducing sugar was analyzed by DNS (dinitrosalicylic acid) method using UV-visible
spectroscopy. The reduced mass of substrate, change of color, alteration of pH of filtrate,
and functional group analysis using Fourier Transform Infrared Spectroscopy (FTIR)
results indicate the decomposition of OPF structures. The highest reducing sugar (3.800
g/L) of the substrate was obtained when the concentration of 0.225 M was used, which is
higher than pretreatment of without FeCl3 addition (2.673 g/L). Overall, our study
concludes that hydrothermal pretreatment assisted by FeCl3 can catalyze the
decomposition of OPF structures to give accessibility of enzyme and enhance the reducing
sugar
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
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28
The potential for using corn husk as agricultural waste for particleboard
raw material
Kurnia Wiji Prasetiyo1*
, Lekianus Zalukhu2, Lilik Astari
1 and Dede Hermawan
2
1Research Center for Biomaterial Indonesian Institute of Sciences Jl.Raya Bogor Km. 46
Cibinong Bogor 2Department of Forest Product Technology Faculty of Forest and Natural Science Bogor
Agricultural University
*Corresponding author: [email protected]
Abstract. The development of particleboard manufactured using non wood raw materials
has become important due to the decreased of wood as main raw material for
particleboard industry. In this study, corn husk as agricultural waste was used for
experimental manufactured of particleboard bonded natural and synthetic adhesives. The
influence of adhesive types and concentration for physical and mechanical properties of
particleboard were investigated. The synthetic adhesive types used were urea
formaldehyde (UF) and phenol formaldehyde (PF) with 8,10 and 12 wt% adhesive
concentration. The natural adhesive used was citric acid with 15, 20 and 25 wt% adhesive
concentration. The target density of particleboard was set at 0.80 g/cm3. The boards were
manufactured at 130 oC for UF, 150 oC for PF and 200 oC for citric acid press
temperature, 10 minutes and 2.5 MPa for pressure of hot press. Generally, the adhesive
types and adhesive concentration were used in this study proven to influence the
properties of particleboard. The results showed that particleboard with citric acid
adhesive has better properties than particleboard bonded UF and PF adhesives.
Keywords: adhesive type, concentration, corn husk, particleboard, Sembilang bamboo
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
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29
The Effect Application of Organic Fertilizer and Dolomit Toward Cassava
Production in Banten Province-Indonesia
Viktor Siagian1*, Ulima Amanda
2, Eko Kardiyanto
3
1,2,3Assessment Institute for Agricultural Technology of Banten, Indonesia
Ciptayasa Avenue Km 01 Ciruas- Serang District, 42182. Telp. 0254-281055,
Fax 0254-282507.
* Corresponding author: [email protected]
Abstract. Cassava (Manihot Esculenta C.) is the third largest crop after rice, and corn in
Banten Province. The objectives of this study are: 1) Determine the effect of organic
fertilizer application and liming on cassava production in Lebak Regency, 2) Study the
difference of cassava farming on the demonstration plot with the cassava farming of
farmers. The research method uses a completely randomized experimental design (CRD)
on a 0.5 ha demonstration plot with 3 treatment plots each with 3 replications. Also with
the survey method by interviewing 11 cassava respondent farmers. The data analysis
method uses qualitative and quantitative analysis. Quantitative analysis uses the ANOVA
(Analysis of Variance) test and the qualitative test uses descriptive tabulations. The
results of this study are: 1) Fcounted = 180.36 > Ftable = 5,143, the conclution id that H0
is rejected, that there is at least one treatment applying organic fertilizer and or liming
which significantly affects the productivity of cassava. 2) The average productivity of
cassava on the demonstration plot is 42.13 tons of wet tubers / ha while at the farmer's
level is 24.18 tons of wet tubers/ha with B/C rasio is 4. The varieties planted are Manggu
Local Superior Varieties both on the demonstration plot and farmers.
Keywords: Cassava Farming, ANOVA Test, production, organic fertilizer, dolomit.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
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Bioconversion of artemisinin by endophytic fungi associated Artemisia sp.
grown in Indonesia and Japan
Shoji Maehara1*, Ahmad Fathoni
2, Muhammad Ilyas
2, and Andria Agusta
2
1Faculty of pharmacy and pharmaceutical sciences,
Fukuyama University, 985, Sanzo, Higashimura-cho,
Fukuyama, Hiroshima, 729-0292, Japan 2Research Center for Biology, Indonesian Institute of Sciences,
Cibinong Science Center, Cibinong-Bogor 16911, Indonesia
*Corresponding author: [email protected]
Abstract. Artemisinin is a well-known anti-malarial drug that can be found in leaf of
Artemisia plant. The plant of Artemisia is growth widely in Europe, North America, North
Africa and Asia. Therefore, Artemisia plant that is called Wormwood or Mugword in
English, consist many plant species. The Indonesian mugwort or “baru cina”, and Japanese
mugwort or “Yomogi” has long been used as culinary herbs and medicinal plant. In this
report, we deal with symbiotic microorganisms in Artemisia plant grown in Indonesia and
Japan. Totally 15 endophytic fungi were isolated form Artemisia from Indonesia, 21
endophytic fungi from Artemisia from Japan. It was found that the composition of each
endophytic fungi varies depending on the growth environment (region). Isolated endophytic
fungi that promote the bioconversion reaction using artemisinin as a substrate have been
clarified by screening tests. Interestingly, it was found that the microbial conversion
reaction of each endophyte was different depending on the growth environment (region).
Here we show reactions by endophytic fungus DnAvCw-1 and Y13-2 associated Artemisia
plant grown in Indonesia and Japan. It was revealed that artemisinin was deoxygenated by
DnAvCw-1 and hydroxylated by Y13-2.
Keywords : artemisinin; endophytic fungi; bioconversion; anti-malaria; Artemisia
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
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31
Isolation of bioactive compounds from Hyptis suaveolens (L.) Poit. and
its antioxidant activity
Swe Swe Aye1*
, Khin Thant Sin2
1University of Yangon , Myanmar
2 Yangon University of Distance Education, Myanmar
*Corresponding author: [email protected]
Abstract. Hyptis suaveolens (Family – Lamiaceae) is a popular medicinal plant used
traditionally for antifertility, anti-inflammatory and antiplasmodial properties. So, This
study was designed to isolate bioactive compounds and to evaluate antioxidant activity of
isolated compounds from Hyptis suaveolens by silica gel column chromatographic
separation, gallic acid (10 .0 mg) was mainly isolated from Hyptis suaveolens.These
isolated compounds were structurally characterized by physico – chemical determination
and spectroscopic techniques such as UV and FTIR by comparing with the reported data..
Moreover, the essential oil was extracted from the fresh leaves of Hyptis suaveolens
by hydro-distillation method and analysed by GC-MS . It was found that
Caryophyllene ,and Sabinene were predominant compounds in essential oil of Hyptis
suaveolens . The antioxidant activity of isolated compounds and essential oil was
determined by DPPH free radical scavenging assay method. The results suggest that the
major compound gallic acid from H suaveolens possess the effective antioxidant
activity.
Keywords: Caryophyllene, free radical scavenging, gallic acid, GC-MS, Hyptis
suaveolens
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
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32
Cosmeceutical Potency of Functional Ripe Cider Buni
Sthefanie Marchellia, Raymond Rubianto Tjandrawinata, and Yanti*
Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta 12930,
Indonesia
*Corresponding author: [email protected]
Abstract. Buni fruit (Antidesma bunius) is a sour seasonal fruit in Indonesia, with limited
availability and only obtainable at certain area. Here, we formulated cider drink from ripe
buni fruits and determine its efficacy for cosmeceutical potency via antioxidant and
collagenase inhibitory activities. Buni ciders were fermented with both single and
combine cultures of Acetobacter xylinum and Saccharomyces cereviseae. Ciders were
further identified by gas chromatography-mass spectrometry (GC-MS) and tested for its
antioxidant activity by the 1,1- diphenyl-2-picrylhydrazyl (DPPH) radical inhibitory
assay, collagenase inhibitorory assay, alcohol content by Conway method, total soluble
phenolic content, and sensory analysis. Buni cider had pH 3.0 and alcohol content up to
8%. Compound profiling showed that buni ciders contained flavonoids, alkaloids, and
organic acids. The antioxidant activity of buni ciders was reached in range of 73-78% and
the total phenolic content in ciders was 7-20 µg gallic acid/mL. Buni cider fermented
from single culture had potential inhibitory effect against collagenase. Sensory profiling
showed that most panelists preferred to drink buni ciders than that of buni juice and
commercial cider. These data indicate that selected ripe buni cider with potential
antioxidant and collagenase inhibitory effects could be applied for cosmeceutical
application.
Keywords: antioxidant activity, cider drink, collagenase inhibitory effect,
cosmeceuticals, ripe buni fruits
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
33
POSTER SESSION
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
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Diversity of Epipelic Diatom in Telaga Pengilon Dieng, Indonesia
Kenanga Sari1*
and Tri Retnaningsih Soeprobowati1,2
1Dept. Biology, Faculty Sciences and Mathematics Diponegoro Univeristy, Jl. Prof. Soedarto, SH,
Tembalang, Semarang, Indonesia 2School of Postgraduate Studies, Diponegoro Univeristy, Semarang 50241, Central Java, Indonesia
Abstract. Telaga Pengilon was affected by acid water with high amount of sulfur from
Telaga Warna, Telaga Pengilon is circumnetral water and has function as irrigation
sourcefor agriculture. the diversity diatom in location where the water moves through the
channel from Telaga Warna is unique. Both of lake are volcanic lake located in Dieng
plateau Indonesia in valley around Prau mountain with elevation 2086 masl, but has
different pHcharacteristic. The research about diatom composition and diversity in plateau
or high montain is adventage to know because the research still limited. Total 103 different
diatom taxa were identified, revealing a circumpolar taxa. Twenty eight taxa Eunotia are
found inthe aquatic ecosystem of Telaga Pengilon and The most dominant genera was
Eunotia formica and Eunotia tropica, Pinnularia was subdominant with 17 taxa. Eunotia
assemblagesoccurs in small acid water bodies indicated that Eunotia has wide range
tolerance for pHfluctuation.
Keyword: diatom, pengilon, diversity, eunotia, dieng
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
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35
Lipase Production by Paecilomyces sp. BK1 on Olive Oil
I Made Sudiana, Atit Kanti, Idris
Research Center for Biology, Indonesian Institute of Sciences, Indonesia
Abstract. Lipase is very important enzyme which catalyze several reaction include
esterification, transesterification, acidolysis and aminolysis. This enzyme are also
important for several industrial production. The objective of study was to evaluate the
effect of substrate, temperature and pH on lipase production under submerge fermentation.
Paecilomyces sp. BK1, Trichoderma viride BK2, Trichoderma sp. BK3, Aspergillus flavus
BK4, Fusarium oxysporum BK5, Paecilomyces chrysogenum BK6, Alternaria alternaria
BK7 were isolated from soil in palm oil plantation. Seven isolates were tested and analyses
was evaluated in tributyrin showing that Paecilomyces sp was the best isolates to produce
lipase. The isolates produce enzyme at pH 7 on 1 % olive oil content media after 72 hours
incubation. The enzyme was stable at temperature 30C until 50C. By the ability of
Paecilomyces to produce lipase and stable at wide range temperature imply that this strain
is potential for lipase production.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
36
Forest Management Prospects Through Development Non-Timber Forest
Products
Gusmailina1)
& Suwardi Sumadiwangsa2)
1)
Forest Products Research and Development Center 2)
Forest Products Research and Development Center Retired
*Corresponding author: [email protected]
Abstract. Non-timber forest products have long been known to be an important
component of the lives of communities around the forest. For most people, non-timber
forest products are an important resource compared to wood. Many households around
the forest area depend on their livelihood mainly on non-timber forest products as a
subsystem and as a main source of income. However, studies from the Worldwide Fund
for Nature (WWF) on NTFP efforts in several regions, show that the very large potential
of NTFPs carried out by communities around the forest is still a side business, even lower
than a side business, and has not been managed optimally. , so as to result in not being
able to contribute meaningful income to meet the needs of his family. Even though
non-timber forest products are prepared to be the new prima donna in the forestry
industry sector in the future. Therefore, the clustering of the forestry industry and the
pattern of partnership management become one of the main weapons to realize these
goals. This paper presents non-timber forest products and their prospects in the future.
Keywords : non-wood forest products, components, community, excellent
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
37
Chemical Characterization of Wood Vinegar Obtained from Acacia Bark
Arief Heru Prianto1,2
, Budiawan1, Yoki Yulizar
1 and Partomuan Simanjuntak
3,4
1Department of Chemistry, Faculty of Natural Science and Mathematics, Universitas
Indonesia, . Kampus UI Depok.16424; Indonesia 2Research Center for Biomaterial-LIPI;
3 Research Center for Biotechnology-LIPI;
Jl. Raya Bogor km.46, Cibinong, Bogor 16911, Indonesia 4Faculty of Pharmacy, Universitas Pancasila,
Jl. Srengseng Sawah Jagakarsa, Jakarta Selatan 12640, Indonesia
Corresponding aurhors: [email protected]; [email protected]
Abstract. The chemical characteristics of wood vinegar produced from Acacia
mangium barks were done. The pyrolysis process was conducted into a low heating rate
400o C using a pyrolysis furnace. It was made of stainless steel, covered by fire clay
bricks, and used electrical heating. Acacia barks were pyrolyzed for 4.5 hours. The
organic acid and phenol were analyzed to determine the quality of wood vinegar. The
chemical components of wood vinegar were identified by gas chromatography-mass
spectrometry (GC-MS). The carbonation process produced charcoal, wood vinegar, gas,
and tar, i.e.56.0, 30.0, 11.1, and 2.93%, respectively. The major component of wood
vinegar were acetic acid (47.4%) and phenolic compounds (16.3%), while tar was
dominated by pentanoic acid (90.3%)
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
38
Optimization of Pulp Concentration for Bioethanol Production from
Elephant Grass (Pennisetum purpureum) with Addition Tween 20 and
Using Two Commercial Yeasts
Ina Winarni1* and Dwi Wahyuni
2
1Forest Product Reseach and Development Center,
FORDA, Ciomas-Bogor 16610, Indonesia 2Faculty of Matemathics and Natural Scince, Pakuan University
Tegal lega, Bogor 16143, Indonesia
*Corresponding author: [email protected]
Abstract. The higher consumption of fuel, especially fossil fuels, will lead to depletion of
fuel reserves and high air pollution which causes environmental pollution. Therefore, it is
necessary to find alternative fuels that are environmentally friendly and abundant
availability in nature so called biofuels. One type of biofuel is bioethanol. Raw materials
that can be produced into bioethanol are biomass or lignocellulosic material. Elephant
grass is one type of lignocellulosic material which has high cellulose content (40.85%).
The objective of this study was to determine the optimum pulp concentration by adding
Tween 20 to the saccharification process with two types of commercial yeasts (fermipan
and mauripan) in the fermentation process. The pulp variations used are (20, 25 and 30%
dw) with Tween 20 concentrations (0% and 1% v). The results showed an increase in pulp
concentration and the addition of Tween 20 by 1% could increase the ethanol content
compared to controls (Without Tween 20). The highest ethanol content was produced at
30% pulp concentration (13.09%) with addition 1% of Tween 20 in the saccharification
process and fermipan yeast in the fermentation stage; and could achieved ethanol content
of 13.94%(v) when using Mauripan yeast.
Keywords : lignocellulose, elephant grass, bioethanol, fermipan, mauripan
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
39
Two Chemotypes of Sindora leiocarpa Essential Oil and Evaluation of Its
Antimicrobial and Antioxidant Activity
Dewi Wulansari1, Kartika Dyah Palupi
1, Praptiwi
1, Hiroshi Kamitakahara
2, Toshiyuki
Takano2, and Andria Agusta
1*
1 Research Center for Biology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46,
Cibinong 16911, Indonesia 2 Graduate School of Agriculture, Div. Forest & Biomaterials Science, Kyoto University,
Japan
*Corresponding author: [email protected]
Abstract. Sindur oil is resinous wood oil from Sindur tree (Sindora leiocarpa). In Central
Kalimantan, Sindur oil is utilised to treat the wound and burn. This study aimed to reveal
the chemical constituent of Sindur essential oil (EO) and its antioxidant and antimicrobial
activity. The chemical components of the EOs were analysed by GC-MS method.
Antioxidant was evaluated using free radical DPPH, while antimicrobial assay was
performed using the broth microdilution method. Two chemotypes of Sindur EOs (A and
B) were successfully identified in which differ in its beta-cubebene, (-)-caryophyllene
oxide-type 1, and two unknown compounds content. Alpha-copaene was identified as a
major compound in both chemotypes. The two chemotypes of Sindur EO were tested
against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Bacillus
subtilis, and Candida albicans with range concentration of 0.098 – 12.5 %. Those two EOs
showed different activity which represented by MIC value. Type A EO was more active
against S. aureus with MIC of < 0.098 µg/ml, while type B was more active against S.
epidermidis and C. albicans with MICs value of < 0.098 and 6.25 µg/ml, respectively.
Both types of Sindur EOs did not show free radical DPPH scavenging activity at the
maximum concentration tested, 500 µg/ml.
Keywords : Sindur oil, Sindora, Sindora leiocarpa, antimicrobial
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
40
SNAP (Single Nucleotide Amplified Polymorphism) Marker Development
Based on Eg-Le (Length) Gene for Early Identification of Low Height
Increment in Oil Palm
Annisa S. Salsabila1* Diny Dinarti1 Roberdi2 and Sudarsono
1
1Division of Plant Breeding and Biotechnology, Graduate School, Bogor Agricultural
University, Bogor-West Java 16680, Indonesia 2PT. SMART Tbk, Cijayanti, Babakan Madang, Bogor-West Java 16810, Indonesia
*Corresponding author: [email protected]
Abstract. Controlling plant height increment of oil palm is one of the strategies to increase
the productivity and slow down the oil palm plantation expansion. The 3β- hydroxylase
(GA3ox) encoded by the LE gene is one of the enzymes involved in the final stage of
Gibberellin biosynthesis. In a pea, plant carrying the mutant of le gene was as short as 40%
of the wild-type one. This research aimed at identifying and determining SNP of Eg-LE
gene and used the identified variability to generate functional SNAP markers. Fragments
of the LE gene were amplified from E. Guineensis (Eg), E. oleifera (Eo) and the Eo x Eg
interspecific hybrids. The PCR amplified products were sequenced and SNAP primer sets
were developed from the identified SNPs using WebSNAPER software. Selected primers
then evaluated for an allele-specific amplification. Results of the sequencing identified a
total of 11 Single Nucleotide Polymorphism (SNP) positions within the coding sequences
of the Eg-LE gene. Eleven SNAP primer sets were tested against 69 oil palm genotypes.
The one SNAP marker based on 1008th loci were associated with the low plant height
increment character in the adult oil palm. Therefore it could be used to support in oil palm
breeding program.
Keywords : dwarf, ga3ox, gibberellin biosynthesis, snap marker, oil palm selection
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
41
Diversity Of Stands Type At Buffer Zone Karst Citatah
Muhamad Yusup Hidayat
1Center for Research and Development of Quality and Environment Laboratory, Ministry of
Environment dan Forestry, Serpong-South Tangerang 15314, Indonesia
*Corresponding author: yusup. [email protected]
Abstract. Karst area is an ecosystem type that has one important function as water
conservation. It is important to know the composition and structure of the stand of karst
area. This study aims to determine the diversity of stands and their potential in Citatah karst
buffer zone. Data of stands composition were collected by using plot purposively on public
forest land owned by the community, spread in three villages. Vegetation analysis was
conducted by census (100%) in all selected stand areas. Index of Importance Value (INP)
showed that Sengon stands (Paraserianthes falcataria) were dominant, both of
regeneration level (79,23) and tree level (165,71). Index of Diversity of the regeneration
level is very stable as it has a range of H '> 2 compared to the tree level that has a range of
H' value 1-2. Index of dominance is only seen at the tree level, That is the Sengon
(Paraserianthes falcataria) stands, C value is one. At the level of regeneration, there is no
visibility of domination one species to other species. Index of similarity at regeneration
level and tree level is quite high (> 55%). Regeneration at the regeneration level grows well
until it reaches the tree level. The pattern of management that suitable acording to the
vegetation structure in the research area is public forest with mixed garden system.
Keywords : Karst Area, Index of Importance Value, Index of diversity, Index of
domination and index of simialirity, public forest
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
42
Phytochemical Content and Antioxidant Activities of Medicinal Plants from
North Sumatra
Gunawan Pasaribu1* and Totok K. Waluyo
2
1,2
Forest Product Research and Development Center,
Jl Gunung Batu No 5 Bogor , Indonesia
*Corresponding author: [email protected]
Abstract. This paper is based on ethnopharmacological investigation in KPHL XXII
Tobasa Labura, North Sumatra. The method of study is conducted through indeept
interviews on local people. The phytochemical compound was qualitatively examined in
the methanol crude extract included flavonoid, tannin, saponin, triterpenoid, steriod,
hydroquinon and alkaloid. The antioxidant activity of the extract was based on the
scavenging activity of the stable 1, 1-diphenyl-2-picrylhydrazyl(DPPH) free radical. There
are ten important species of medicinal plant have collected. The local people used the
medicinal plant of various disease or disorder such as gastrointestinal disorder,
antidiabetic, wound healing, fever, stomach ache and antiinflamation. The phytochemical
testing showed that the extract contains flavonoid, saponin, tanin and alkaloid. The
antioxidant activity (IC50) showed that Sikkam 27,06 ppm; Modang Kulim 104,81 ppm;
Handis 35,38 ppm; Rugi-rugi 45,70 ppm; Sambang-sambang 207,63 ppm; Tolong 58,97
ppm; Sae-sae putih 53,19 ppm; Sae-sae hitam 41,08 ppm; Pirdot 146,40 ppm and
Sirungguk more than 250 ppm. In general, the antioxidant activity of ten medicinal plant
species studied had good activity.
Keywords : Antioxidant, ethnopharmacology, plant, pyhtochemical, Sumatra
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
43
The Comparison of different diatom digestive method using HCl, H2O2 and
HCl, KMNO4 in Telaga Pengilon Dieng
Christopher Hardian Widyanta1*
and Tri Retnaningsih Soeprobowati1,2
1Dept. Biology, Faculty Science and Mathematics Diponegoro University, Jl. Prof. Soedarto,
SH, Tembalang, Semarang, Indonesia 2School of Postgraduated Studies, Diponegoro University, Semarang50241, Central Java,
Indonesia
Abstract. Identification diatom has been applied as an important key to trace
paleoenvirontmental condition. The knowledge for diatom extraction from sediment is
still continue, but, the diatom digestive methods in tropical area is still limited. Telaga
Pengilon is located in Dieng Indonesia rich of organic material in the sediment, the
sample also associate with epifitic and benthic diatom. Therefore, extracting pure diatom
frustules free of organic matter from the sediments is an essential requirement for this
application. To find the appropriate method for extracting diatoms in Telaga Pengilon,
this research compares HCl with H2O2 and HCl with KMNO4 as the digestive reagent.
Based on statistic result (HCL, KMNO4) less time consumed than (HCL, H202) during
digestive process (p<0.05) and produced same amount residu (p> 0.05). For Pinnularia
and Frustulia , the majority of diatom could be extracted using both of methods. Under
microscopy, the structure of diatoms remained almost perfect after digestion with H202,
the striae clearly visible and the residu digest perfectly. Another using HCL,KMNO4
fragmentation was found for some Pinnularia and for Frustulia the striae is clearly visible.
This study demonstrated that different diatom has different resistance for reagent and
some of location need different regent because has different material contain in the
sediment and need help to remove material organic. As far as the HCL, and H2O2 is
appropiate methods for digestive in tropical area such as Telaga Pengilon, HCL,KMN4
can be substitute for less time consuming process.
Keyword: Diatom, digest, methods, HCL, KMNO4, H2O2
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
44
PE Goat Performance in the Breed Stock Area, Banjarmangu District,
Banjarnegara Regency, Central Java Province
Tatan Kostaman1*, Angga Ardhati Rani Hapsari
1, Singgih Setiawan
2, Bess Tiesnamurti
2, and
Anneke Anggraeni1
1Indonesian Research Institute for Animal Production (IRIAP),
Jl Veteran III Banjarwaru, Ciawi-Bogor 16720, Indonesia 2Indonesian Center for Animal Research and Development (ICARD),
Jl. Raya Pajajaran Kav E-59, Bogor 16151, Indonesia
*Corresponding author: [email protected]
Abstract. Banjarmangu Subdistrict, Banjarnegara District, Central Java Province has been
designated as a breeding stock for PE goats. The purpose of the study was to determine the
performance of PE goats in the breeding area. The study was conducted using a survey
method, interviews with goat breeders about reproductive characteristics, number of
ownership, and livestock management accompanied by observations to the breeder's
location. The respondents interviewed were 24 people. Criteria for determining
respondents are livestock farmers who have original PE goats. The results showed that the
current population averages based on the sex ratio of male and female adults are not ideal
which is 2:3. Meanwhile, the sex ratio of the male and female kids which is 1:1. The mating
management is still carried out naturally, that is female animals that are being lured
together with superior males. The selection system is carried out by farmers based on the
quality of the kids produced. The maintenance system is carried out intensively.
Management of animal health is handled by animal health workers together with breeders
and the disease that often arises is scabies. It was concluded that the performance of PE
goats in the seed source area needs to be improved in quality, namely by the introduction of
technology.
Keywords : PE goats, performance, breed stock
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
45
Screening Phytochemical Compound of Alstonia scholaris R.Br in Different
Sites in Indonesia
Zuraida1*
and Sulistiyani2
1Forest Research and Development Centre, Environment & Forestry Research Development
and Innovation Agency (FORDA), Ministry of Environment and Forestry, Jalan Guning Batu
no 5 Bogor 16610, Indonesia 2Biochemistry Department, IPB University Bogor, Jl. Raya Dramaga, Bogor 16680
*Corresponding author: [email protected]
Abstract. Indonesia's tropical forest is widely used as a source of raw materials of
traditional medicine. Pulai (Alstonia scholaris R.Br) is one of medicinal plants widely
spread in Indonesia to treat various diseases. Phytochemical compounds play important
role in farmacology effect, which depend on many factors such as age, geographical site,
solvent and extraction methods.These factors result in different phytochemical
compounds, and biological activity. The aimed of this research was to investigate
phytochemical content of Alstonia scholaris’ bark from different region in Indonesia by
maceration and reflux extraction methode. Alstonia scholaris bark‟s extract were tested its
phytochemical content for alkoloids, flavonoids, tannins, steroid saponins and
triterpenoids. The results showed that pulai bark extracted by maceration was bigger in
yield than reflux method. Phytochemical tested showed that all samples positive
flavonoids, and alkaloids. Alstonia scholaris’ bark from Cifor-Bogor had the strongest
flavonoids content of all. Flavonoid and alkaloid aree phytochemical compound play
important role as antioxidant used for anti aging, aterosklerosis, diabetes, and
inflammation.
Keywords : Alstonia sp.; flavonoids; maceration; phytocemical; reflux
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
46
Current Status and Species Diversity of Seagrass on Panjang Island,
Banten
Yayuk Sugianti1*
and Mujiyanto1
Research Institute for Fish Resources Enhancement
Jalan Cilalawi No. 1 Jatiluhur, Purwakarta, Indonesia
*Corresponding author : [email protected]
Abstract. Damage to seagrass beds on Panjang Island, Banten, has increased every year.
The most significant decline occurred at an interval of 2000-2005 with a decrease of
about 22.9 ha. Seagrass damage continued to increase at the year between 1989-2002 as a
result of natural stone mining and coastal reclamation activities to become industrial
areas and ports. The objective of this study was to determine the characteristics of the
species and current status of seagrass communities in Panjang Island waters, Banten. The
study was conducted on October 2015 and April 2016 located in Panjang Island waters,
Banten. Analysis of seagrass data included identification of species, frequency of seagrass
species, calculating the density, percentage of the seagrass coverage, and important value
index. Based on these results, it can be seen the level of current seagrass conditions. PCA
analysis was also conducted to determine the relationship of seagrass density with
chemical physics parameters. The results obtained showed the condition of seagrass
ecosystems in Panjang Island waters, Banten, which was at a level of moderate to severe
damage. The results of PCA analysis showed that water temperature, nitrate concentration,
DO, and TSS greatly influenced the density of seagrass in these waters.
Keywords: Banten, current status, seagrass, species diversity, Panjang Island
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
47
Effect of Coconut Water and Extract of Banana Hump on The Growth of
Binahong (Anredera cordifolia (Ten) Steenis) Accessions from the
Lowland
Yaya Hasanah*1)
, Lisa Mawarni1)
, Chairani Hanum1)
, Nurhaida
1)
1)
Faculty of Agriculture, Universitas Sumatera Utara
Jl. Prof. A. Sofyan No 3 Kampus USU, Medan 20155 Indonesia
*Corresponding author : E-mail : [email protected]
Abstract. Application of natural plant growth regulators (PGR) influences the growth of
binahong. The objective of the research is to evaluate the growth component of lowland
binahong accessions due to the application of natural PGR of coconut water and banana
hump extract. The research used factorial randomized block design with 2 factors and 3
replications. The first factor is accession of binahong from lowlands (Marelan; Ampera
Tmur ; Helvetia) in Medan. The second factor is the application of natural PGR, namely
without the application of PGR ; 40% coconut water application; banana hump extract
40% ; coconut water 40% + banana hump extract 40%. The results showed that the
accessions from Ampera Timur had plant length, shoot dry weight and root dry weight that
were higher than the other accessions. The treatment of 40% coconut water + 40% coconut
water extract increased plant length, and root dry weight. Interaction of Helvetia binahong
accession treatment and 40% coconut water + banana hump extract 40% increased plant
length of binahong.
Keywords : binahong, accesion, coconut water, banana hump
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
48
Evaluation of The Potency of Endophytic Fungi Associated with Artemisia
annua and Artemisia vulgaris as Antibacterial and Antioxidant
Evana1, Praptiwi
1, Ahmad Fathoni
1, Muhammad Ilyas
1, Shoji Maehara
2 and Andria Agusta
1*
1Research Center for Biology, Indonesian Institute of Sciences,
Cibinong Science Center, Cibinong-Bogor 16911, Indonesia 2Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University,
Sanzo, 1, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan
*Corresponding author: [email protected]
Abstract. Artemisia annua and Artemisia vulgaris have been known as traditional herbal
medicine. The purpose of this study was to evaluate the potential of 8 endophytic fungi
associated with A. annua, and 16 endophytic fungi associated with A. vulgaris as
antibacterial and antioxidant. Preliminary screening for antibacterial and antioxidant
activities were carried out by the TLC-bioautographic method, and the active extracts were
further analyzed for their MIC and IC50 values by serial microdilution method at 96
microwell plates. The results showed that 23 endophytic fungi were able to inhibit
Staphylococcus aureus with MIC values ranging from 64 to > 256 µg/ml, 20 endophytic
fungi inhibit the growth of Escherichia coli with MIC values of 128 to > 256 µg/ml. Two
isolates have very strong antibacterial activity against S. aureus (MIC = 64 µg/ml) were
DnAvCb-3 and TdAaCb-2. Sixteen endophytic fungi extracts were active as antioxidant by
acting as DPPH free radical scavengers. The results of this study indicated that the
endophytic fungi extracts associated with A. annua and A. vulgaris were promising natural
sources for antibacterial and antioxidant.
Keywords : Artemisia annua, Artemisia vulgaris, endophytic fungi, antibacterial, DPPH
antioxidant.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
49
Distribution of Long-Tailed Monkeys (Macaca fascicularis) in Kelimutu
National Park
Ridwan Fauzi1,2
*, Tonny Wuryanto1,3
, Endarto1,4
, Fendra Suarmadi1, and Albertus Tomonob
1
1 Kelimutu National Park, El Tari Street No. 16, Ende-Flores, NTT
2 Research and Development Center for Environmental Quality and Laboratory, Ministry of
Environment and Forestry, Kawasan Puspiptek Gedung 210, Serpong, Tangerang Selatan 3 Directorate for Management and Information on Nature Conservation, Ministry of
Environment and Forestry, Jl. Ir Juanda No 15 Bogor 4Baluran National Park, Jl. Raya Situbondo Banyuwangi KM.35, Kec. Banyuputih, Kab.
Situbondo, Jawa Timur
*Corresponding author: [email protected]
Abstract. Long-tailed monkey (Macaca fascicularis) is one of mammal that lives in
Kelimutu National Park (Kelimutu NP). This mammal in their natural habitat has an impact
on buffer zones in the form of animal activities that damage crops on plantation lands. The
purpose of this study was to identify the distribution of long-tailed monkey populations.
This research was conducted in August 2010 by covering areas within the region and
outside the Kelimutu NP area. Data analysis was used to calculate the abundance index, the
encounter rate for estimating abundance and distribution based on the length of the track
surveyed. The results indicates that the distribution of long-tailed monkey populations in
Kelimutu NP was most widely found in field habitats, with relatively low abundance of
around 5 individuals/ km2. Based on the calculation of habitat carrying capacity for groups
of monkeys in the tourism zones, it is still below the maximum limit. Increased awareness
of ecological insight tourism also needs to be optimized related to the presence of monkeys
in the tourism zones of Kelimutu NP. Development of habitats, such as planting of trees for
foods and protected zones is necessary to provide suitable habitat for monkeys.
Keywords : Macaca fascicularis, Kelimutu National Park, protected zones
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
50
Local Rice Conservation Through the Local Wisdom of Indigenous Culture
at Kasepuhan Cisungsang Banten Province
Pepi Nur Susilawati, Zuraida Yursak and Ulima D Amanda
Peneliti Balai Pengkajian Teknologi Pertanian Banten
Jl. Ciptayasa KM.01 Ciruas –Serang Provinsi Banten
Email : [email protected]
Abstract. Local wisdom is very important in conservation of local rice. Utilization of
local rice varieties has been carried out in the specific groups of people for centuries
under local culture rules (Kasepuhan Cisungsang). Cisungsang culture is one of the local
cultures in Banten Kidul with indigenous people who still maintain local wisdom in rice
cultivation. The research objective is to observe the role of Cisungsang indigenous
culture in preserving local rice germplasm. The study was conducted in Kasepuhan
Cisungsang, Cibeber District, Lebak Regency, Banten Province. The data used were
primary and secondary data, with survey and desk study. The results showed that the law
of Cisungsang culture applies not only to people and community life but also to
agriculture especially for rice cultivation. Rice, manifested as goddess “Dewi Sri”, has a
noble position for indigenous people as it must be properly nurtured and nourished in
order to provide optimal harvest. There were 30 varieties of local rice varieties developed
in Kasepuhan Cisungsang and surrounding areas, but only 10 varieties were planted by
the community: Marileun, Terong Papak, Cireh Gudang, Kapundung, Tampai Beureum,
Srikuning, Tampey Bodas, Nemol, Gadog, Angsana. The ten varieties were in accordance
with the farmer preferences and adaptive strength in the local agroecosystem.
Keywords: upland rice, local wisdom, kasepuhan cisungsang, Banten Kidul
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
51
In vitro response of patchouli in a medium by addition of
Methylobacterium spp filtrate
Mia Kosmiatin1* and Seli Salma
2
1Indonesia Center of Agricultural of Biotechnology and Genetic Resources Research and
Development, ICABIOGRAD, Jalan Tentara Pelajar No 3A, Bogor, 16111, Indonesian 2Indonesia Soil Research Institute, Jalan Tentara Pelajar No 12, Bogor 16114, Indonesia
*Corresponding author: [email protected]
Abstract. Mass providing superior varieties, are become important to support modern
farming. Propagation by in vitro technique could be solving that problem, but the price
determined by medium formulation. The addition of synthetic PGR has been a residual
effect, meanwhile natural PGR more secure and expensive than the synthetic ones. In
vitro testing of PGR activity obtained from bacteria was be the first step to substituted
synthetic PGR to become nature PGR. Research to observe PGR activity obtained from 4
strains of Methylobacterium spp from East Kalimantan that had been analyzed of the
capability of PGR synthesis was be done on in vitro patchouli cultured. There are 3
activities: 1. The sterilized technique of Methylobacterium spp filtrate; 2) Testing of
Methylobacterium spp from that production of cytokinin; and 3) auxin. The research
showed that strain TD-J2 filtrate added to medium had been similar and better to cytokinin
activity and strain TD-J7similar to zeatin activities to callus induction and could be shoot
regenerated. Auxin activity derived from strain TD-J10 showed the capacity to induces
root formed. The research result indicates that synthesis PGR derived from
Methylobacterium spp. has similar activity with synthetic PGR which commonly used in
plant in vitro culture.
Keywords: Patchouli, in vitro culture, Methylobacterium spp, plant growth regulators
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
52
Legislation on genetically modified animals and its application possibility in
Indonesia
M. Ikhsan Shiddieqy1*, Bess Tiesnamurti
1 and Sri Muharsini
2
1Indonesian Center for Animal Research and Development
Jalan Pajajaran Kav E59, Bogor 16151, Indonesia 2Indonesian Research Center for Veterinary Sciences
Jalan RE Martadinata No. 30 PO BOX 151, Bogor 16114, Indonesia
*Corresponding author: [email protected]
Abstract. The government is responsible for fulfil the needs of safe, healthy and halal food
for all its people in a sustainable manner. The world population will increase from 7 to 9
billion people in 2050. While the availability of land may not increase in the future, it may
even not used for agriculture. Scientists are trying to overcome this problem by creating
technologies that can increase livestock productivity, one of which is through a genetically
modified process. Genetically modified animals are animals that have been genetically
modified for a many of purposes including yields and disease resistance. Although
genetically modified animals are at the research stage and the possibility to entering
Indonesia remains small, but government should prepare the regulations related to it.
European countries and USA have regulations to control genetically modified animals.
Malaysia also has similar regulation since 2007. Currently, Indonesia has formed an
authority that oversees the genetically modified product, namely Biosafety Commission
(Komisi Keamanan Hayati). However, this commission only supervises genetically
modified products in food and feed. This paper provides general review on regulations
related to genetically modified products and detailed suggestions on regulation regarding
genetically modified animals
Keywords : Genetically modified products, animals, regulation
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
53
Optimization of Xylose Production from Sugarcane Trash by
Microwave-Maleic Acid Hydrolysis
Euis Hermiati1, Maulida Oktaviani
1, Riksfardini Annisa Ermawar
1, Raden Permana Budi
Laksana1, Lutfi Nia Kholida
2, Ahmad Thontowi
2, and Siti Mardiana
3
1Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor
Km 46, Cibinong, Bogor 16911, Indonesia 2Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor
Km 46, Cibinong, Bogor 16911, Indonesia 3University of Lampung, Jl. Prof. Dr. Sumantri Brojonegoro No. 1, Bandar Lampung 35145,
Indonesia
*Corresponding author: [email protected]
Abstract. Sugarcane trash contains significant amount of xylan that could be hydrolysed to
xylose. The xylose could be further fermented to produce xylitol, a sugar alcohol that has
low calories and does not cause carries of teeth. In this study we optimized the production
of xylose from sugarcane trash by microwave-assisted maleic acid hydrolysis using
response surface methodology (RSM). The factors optimized were acid concentration,
time, and temperature. The xylose concentration obtained was determined and it served as
a response variable. Results show that acid concentration has significant effect on xylose
production, and the model generated from the optimization was fit and can be used to
predict the xylose concentration in the hydrolysate with various combinations of acid
concentration, time, and temperature. The optimum condition for xylose production from
sugarcane trash was using maleic acid of 1.7%, and heating at 177 °C for 6.7 min. At this
condition there was 25 g/L of xylose could be produced.
Keywords : Maleic acid, microwave heating, response surface methodology, sugarcane
trash, xylose
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
54
Estimation of Biodiesel Production from Used Cooking Oil of University
Canteen to Support Sustainable Electricity in Universitas Pertamina
Ari Rahman1*, I Wayan Koko Suryawan
1, Ariyanti Sarwono
1, Nurulbaiti Listyendah Zahra
1,
Ziyadatulkhair M. Faruqi2
1Faculty of Infrastructure Planning, Department of Environmental Engineering, Universitas
Pertamina, Komplek Universitas Pertamina, Jalan Sinabung II, Terusan Simprug, Jakarta
12220, Indonesia 2Undergraduate Program, Department of Environmental Engineering, Universitas Pertamina,
Komplek Universitas Pertamina, Jalan Sinabung II, Terusan Simprug, Jakarta 12220,
Indonesia
*Corresponding author: [email protected]
Abstract. Universitas Pertamina is a new private university founded in 2016. The
university has a vision to be a world-class university leading in the energy sector. In
achieving its vision, the university began to carry out various programs related to energy.
One such program that is being promoted is the use of renewable energy resources. The
objective of this study was to investigate the used cooking oil, obtained from the university
canteen as an alternative feedstock for producing biodiesel fuel. Biodiesel is produced
through a chemical process called transesterification. Based on the survey questionnaire,
the university canteen produces on average 36 L/day of used cooking oil. The amount of
biodiesel produced was 30.2 L/days with 83.9% of yield. Using a diesel testing machine,
biodiesel with B30 type (30% biodiesel and 70% diesel in volume) holds a potential to
generate electricity of 3,021.2 KWh/month. This value able to contribute 12% to lighting
the Rectorate Building for university administration and official activities. Savings of Rp.
3,348,857 can be achieved every month by substituting diesel fuel for biodiesel. Moreover,
by considering biomass as sustainable renewable energy resources, a reduction in carbon
emissions can be valued by 2.2 MTCO2/month.
Keywords: Biodiesel, Carbon emission, Electricity, Universitas Pertamina, Used cooking
oil
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
55
The Effect of Sucrose Concentrations and Shoot Inoculum Density on The
Growth of In Vitro Shoot of Teak (Tectona Grandis L.) **
Witjaksono*, Indira Riastiwi, Katarina Utami Nugraheni dan Gut Windarsih1
Plant Cell Tissue Culture Laboratory, Botany Devision, Research Center for Biology,
Indonesian Institute of Sciences, Jl. Raya Bogor Jakarta Km 46, Cibinong, Bogor 16911
1Universitas Islam Negeri Sultan Maulana Hasanuddin,
Jl. Syech Nawawi Al Bantani Andamui, Curug, Serang 42116, Banten
*Corresponding author: [email protected]; [email protected]
**All authors contributed equally to this work
Abstract. The teak belongs to the highly prized timber tree. Quality teak trees have been
guaranteed by planting clonal planting materials propagated vegetatively, for example by
shoot tip cutting and tissue culture. Tissue culture propagation of teak has been
commercially viable, eventhough efficiency of production can still be improved. This
research aims to study the effect of increasing shoot inoculum density and sucrose
concentration requirement for optimum growth of teak shoot in vitro. Inocula of a single
nodal shoot in the number of 5 or 9 were inoculated on modified MS basal media with
sucrose concentrations treatment of 20, 30, 40, 50 and 60 g l-1. The results showed that
standard sucrose concentration of 30 g l-1 was optimum for the growth five inocula,
while for nine inocula, the optimum sucrose concentration was 40 g l-1. Eventhough the
average shoot growth was lower for nine inocula than that of five inocula, the total shoot
growth was higher for the former. Increasing number of inocula can be used as a method
to increase the efficiency of shoot proliferation.
Keywords: teak, micropropagation, in vitro, sucrose, inoculum density, shoot culture
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
56
Screening of Antibacterial and Antioxidant Activities of Stingless Bee
(Tetragonula laeviceps Smith) Extracts Collected from Purwodadi Botanical
Garden – East Java, Indonesia
Oscar Efendy1,2*
, Ahmad Fathoni1, Praptiwi
1, Dewi Wulansari
1, Wibowo Mangunwardoyo
2,
and Andria Agusta1
1Research Center for Biology, Indonesian Institute of Sciences, Jl. Raya Jakarta – Bogor KM
46, Cibinong 16911 2Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Indonesia,
Campus UI Depok, West Java 16425, Indonesia
*PhD student, Corresponding author: [email protected]
Abstract. This study was conducted to determine the potential of Tetragonula laeviceps
methanol extract and fractions (hexane, dichloromethane, ethyl acetate, methanol, and
acetate-water) as an antibacterial, antioxidant and β-glucosidase inhibitor. The test was
carried out by thin layer chromatography (TLC) - bioautography (dot-blot and elution).
Antibacterial tests were carried out against Staphylococcus aureus and Escherichia coli,
antioxidant potential was carried out by DPPH method. The results showed crude extract,
hexane and dichloromethane fractions had moderate antibacterial activity against S. aureus
(MIC values: 128-256 ug/ml) tetapi weak antibacterial activity against E.coli. The extract
and fractions of dichloromethane, methanol and aceton-air had very strong antioxidant
activity, while dichloromethane and ethyl acetate extracts had activity as β-glucosidase
inhibitors. The results showed that the klanceng hive extract (T. laeviceps) had bioactivity
as an inhibitor of β-glucosidase, antibacterial and antioxidant. Further research to
determine the potential and isolation of active compounds needs to be done. So, it can be
explored further as new sources of antioxidant, antibacterial and β- glucosidase inhibitor.
Keywords: Stingless bee, TLC-bioautography, antibacterial, antioxidant
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
57
Diversity of The Ecletus Parrot (Ecletus roratus) Birds Inferred From
Mitochondraial Gene Sequences
Dwi Astuti
Research Center for Biology, Indonesian Institute of Sciences
Gd. Widyasatwaloka, Jl. Jakarta – Bogor KM 49, Cibinong Science Centre, Cibinong West
Java.
e-mail : [email protected]
Abstract. Electus parrot (Eclectus roratus) Bird is included in the group of parrots, the
Psittacidae family. Body size is 35-42 cm; and weighs is 355–615 g. Eclectus bird have 9
subspecies. Their distribution is in the Solomon Islands, Sumba, New Guinea and nearby
islands, northeastern Australia, and the Maluku Islands (Moluccas). Seven of these
subspecies are in the territory of Indonesia. Wild populations is in declining with the
Least Concern category on the IUCN Red List and included in the CITES Appendix. Like
other parrot birds, this bird has high economic value so its existence needs to be
preserved for used sustainably. The study of the genetic diversity of these birds was
revealed in this study based on DNA sequences of mitochondrial cytB gene. This
information might be needed for their conservation efforts. Blood samples of 0.1 cc were
taken from 13 birds in the zoo and in captivity. DNAs were extracted from each blood
sample and then DNA fragments from the mitochondrial cyt b gene through a PCR
process and then the nucletide was sequenced. DNA sequence data from the cytb gene
were analyzed using specific software to get information about variations, genetic
diversity and grouping of individual birds. Of the 720 cyt-b base pairs there were no stop
condons, there were 7 base positions that vary starting at 112 base site and ending at 616
base side. Genetic distance between individual birds ranges from 0,000 to 0.012. There
were 3 sequence haplotypes with a haplotype diverity (Hd) value of 0.500 ± 0.136 and
Nucleotyde diversity (Pi) value of 0.004431 ± 0.00140, average number of nucleotide
differences (k) 2. 615.
Keywords: Eclectus Parrot Bird, cytB sequences, nucleotide variation, genetic
distance, haplotype n nucleotyde diversities
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
58
Distribution of Long-Tailed Monkeys (Macaca fascicularis) in Kelimutu
National Park
Ridwan Fauzi1,2
*, Tonny Wuryanto1,3
, Endarto1,4
, Fendra Suarmadi1, and Albertus Tomonob
1
1 Kelimutu National Park, El Tari Street No. 16, Ende-Flores, NTT
2 Center for Research and Development of Quality and Environmental Laboratory, Ministry of
Environment and Forestry, Kawasan Puspiptek Gedung 210, Serpong, Tangerang Selatan 3 Directorate of Designing and Information on Nature Conservation, Ministry of Environment
and Forestry, Jl. Ir Juanda No 15 Bogor 4Baluran National Park, Jl. Raya Situbondo Banyuwangi KM.35, Kec. Banyuputih, Kab.
Situbondo, Jawa Timur
*Corresponding author: [email protected]
Abstract. Long-tailed monkey (Macaca fascicularis) is one of mammal that lives in
Kelimutu National Park (Kelimutu NP). This mammal in their natural habitat has an impact
on buffer zones in the form of animal activities that damage crops on plantation lands. The
purpose of this study was to identify the distribution of long-tailed monkey populations.
This research was conducted in August 2010 by covering areas within the region and
outside the Kelimutu NP area. Data analysis was used to calculate the abundance index, the
encounter rate for estimating abundance and distribution based on the length of the track
surveyed. The results indicates that the distribution of long-tailed monkey populations in
Kelimutu NP was most widely found in field habitats, with relatively low abundance of
around 5 individuals/ km2. Based on the calculation of habitat carrying capacity for groups
of monkeys in the tourism zones, it is still below the maximum limit. Increased awareness
of ecological insight tourism also needs to be optimized related to the presence of monkeys
in the tourism zones of Kelimutu NP. Development of habitats, such as planting of trees for
foods and protected zones is necessary to provide suitable habitat for monkeys.
Keywords : Macaca fascicularis, Kelimutu National Park, protected zones
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
59
Classification of Agarwood Product from Riau – Indonesia using Near
Infrared Spectroscopy
Danang Sudarwoko Adi1*, Dwi Ajias Pramasari
1, Bernadeta Ayu W
1, Adik Bahanawan
1,
Yusup Amin1, Sukma Surya K
1, Wahyu Dwianto
1, Hwang Sung-Wook
2 and Junji
Sugiyama2,3
1Research Center for Biomaterials, Indonesian Institute of Sciences,
Cibinong Science Center, Cibinong-Bogor 16911, Indonesia 2Research Institute for Sustainable Humanosphere, Kyoto University
Gokasho, Uji, Kyoto 611-0011, Japan 3College of Materials Science and Engineering, Nanjing Forestry University
Nanjing 210037, China
*Corresponding author: [email protected]
Abstract. Up to know, classification of agarwood product in Indonesia use visualization of
surface colour as well as the odour level, thus the results are qualitative. The aim of this
study is to investigate the potential utilization of Near Infrared (NIR) spectra in
identification of the agarwood products. Five different grades of agarwood from Riau –
Indonesia were used in this experiment. They were subjected to the NIR instrument at
wave number 10,000 – 4,000 cm-1
. Principal component analysis (PCA) and several
classifier, such as k-nearest neighbour (k-NN) and support vector machine (SVM) were
used to analyse the data. Furthermore, random forest was also used to obtain the feature
importance for the classification. All of the program were performed on Python 3.7.3. The
results revealed that all sample were clear distributed on the different cluster of PCA score
plots. The accuracy of k-NN and SVM were also very high and well separated among five
grades agarwood. The application of NIR spectra on agarwood is promising to generalize
the identification of different graded products. However, taking more variation of the
samples to build a data base is suggested to make the classification more accurate and
reliable.
Keywords : agarwood; PCA; classification, NIR
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
60
Identification and Fiber Morphology Measurements of Enggano Island
Woods Species
Wahyu Dwianto1*, Danang S. Adi
1, Teguh Darmawan, Adik Bahanawan, Eka Lestari
1, Yusup
Amin1, Dwi A. Pramasari
1, Fitria, Ratih Damayanti
2, Sri Rulliaty
2, Junji Sugiyama
3
1Research Center for Biomaterials, Indonesian Institute of Sciences,
Cibinong Science Center, Bogor 16911, Indonesia 2Forest Products Research and Development Center, Ministry of Environment and Forestry,
Gunung Batu, Bogor 16610, Indonesia 3Research Institute for Sustainable Humanosphere, Kyoto University
Gokasho, Uji, Kyoto 611-0011, Japan
*Corresponding author: [email protected]
Abstract. The objectives of this exploration were to find, identify and characterize the
potential of lesser-known wood species in Enggano Island, especially for sawn-timbers.
Wood samples were taken by drilling the trees to get a pencil-like shape with size of 20 cm
in length and 0.5 cm in diameter. Identification and anatomical observation of wood
samples were according to IAWA. Preparation of wood fiber maceration followed the
Schultze method, then lumen diameter and fiber length were measured by microscope.
Physical properties of wood were determined to obtain specific gravity and volume
shrinkage. Absolute bending and compression strength, as well as the strength class were
estimated through the specific gravity values based on Indonesian Forestry Classification.
As much as 22 wood species have been collected, where nine of which were lesser-known
wood species. Lesser known wood species, which having potential as sawn timbers with
strength class II were Ki Ahid (Intsia sp.) and Kasai (Kingiodendron sp.); and that with
strength class III were Kabaruk (Antiaris sp.) and Ki Ono (Combretocarpus sp.). From the
fiber morphological measurements showed that the smaller the diameter of the lumen and
the longer the fiber, the specific gravity of wood increased.
Keywords : fiber length; lesser-known wood species; lumen diameter; specific gravity;
strength class
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
61
Optimum Tempertature for Germanation and Seedling Characterization of
The Three Millet Accessions
N Setyowati1*
, P Lestari1 and A H Wawo
1
1Research Center for Biology, Indonesian Institute of Sciences,
Cibinong Science Center, Cibinong-Bogor 16911, Indonesia
Abtract. The research has been conducted at the Physiology Laboratory of Research
Center for Biology LIPI. The study aims to provide information about the prediction of
millet plants distribution based on optimum temperature of germination. Millet
accessions of Polman, Gambirmanis, and Enggano used as research materials. The study
was arranged according to the factorial RCBD. The treatment consisted of 2 factors,
namely accession (Polman, Gambirmanis, and Enggano) and germination temperature
(10,20,30 and 40°C), with 3 replications. The seeds were germinated in Petridish. Each
accessions of millet have distinctive characters. The colour of the dehusked grain affects
the colour of flour that turned to affects the quality of millet products. The grain colours
of Accession Polman were white, Gambirmanis has gray colour, and Enggano seeds were
yellowish. Germination temperature affects seedlings growth. Millet seeds can germinate
well at temperatures of 10-30oC. The optimal germination occurs at 30
oC that indicated
by the optimal seedlings growth. At this temperature, Polman's accession had the best
performance. The seed that germinated at higher temperature (40oC) appeared to be
seized, with a low growth rate. It is assumed that millet can be adapted well in the
lowlands to highland. However, this plant grows optimally in the lowlands with a
germination temperature of 30oC.
Keywords: accession, flour, germination speed, highland, lowland, seed viability.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
62
Pcr Column Contamination Gave False Positive Result for Cherax
Quadricarinatus Densovirus (Cqdv) In Sf 9 Cell Cultures
Dewi Syahidah1*, Jennifer Elliman
2, and Leigh Owens
2
1 Pathology Laboratory, Institute for Mariculture Research and Fisheries Extension
(IMRAFE) Gondol, 81155 Indonesia
2 College of Public Health, Medical and Veterinary Science (CPHMVS) JCU, 4814 Australia
*Corresponding author: [email protected]
Abstract: The DNA binding columns used in many extract kits to extract and purify have
been shown in previous studies to be responsible for introducing foreign DNA such as
mouse DNA into test samples. This present study aimed to in vitro propagation C.
quadricarinatus densovirus (CqDV) in Spodoptera frugiperda cell cultures (Sf9). The
infection of CqDV was detected with a routine histology by H&E staining and with
molecular analyses by PCR. Sf9 was sub-cultured in 6 75TC flasks with Sf-900 II SFM
media and Streptomycin (5ml/l). RT-PCR was performed using primer set of
CqPV5F-CGC TGT GGA GAG TGC ACT AGA G- and 2ARCR- TCT GAA TCA ATC
TCC TCA CG-. Infected cells (80% monolayer) received virus inocula (200µl) from
prepared virus-inocula of CqDV-infected redclaw crayfish (C. quadricarinatus) at JCU
Australia, whereas 1xPBS (200µl) was added into uninfected cells (80% monolayer). Both
cell groups were passaged every 5 days when cells are fully monolayer. Fifty percent of
cells‟ suspension from each flask were collected for 12 passage times and subjected to HE
and PCR analyses. The results showed that HE stains failed to show the effect of virus
infections in infected cell groups. However, the amplification of both infected and
negative cell groups showed positive results (280bp). Therefore, we extract and test our
non-template control samples (NTC) to detect any possible contamination of our PCR
columns. Interestingly, we find out that the PCR amplification of our NTCs also positive
for 280bp. This false positive result showed contamination in our PCR columns used. A
non-column PCR method might give more accurate positive results for CqDV.
Keywords: PCR column, contamination, CqDV, Sf9
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
63
Economic Valuation of Forest Fruits Utilization in Batak Toba Community
Alfonsus H Harianja1,2
, Anisse M. Sinaga3, Ferry A Hawari
4 and Ridwan Fauzi
5
1,5
Research and Development Center for Environmental Quality and Laboratory, Forestry and
Environmental Research, Development and Innovation Agency, Ministry of Environment and
Forestry of the Republic of Indonesia, PUSPIPTEK, Building 210 Serpong, South Tangerang,
Indonesia. 2 e-mail: [email protected]
3Aek Nauli Environmental and Forestry Research and Development Agency
4Orangutan Information Centre, OIC
Abstract. Batak Toba community that settled around forests utilize forest-fruit grown
around their settlement. This research aims to quantify the economic value of the
forest-fruit using Local User Value Index (LUVI) assessment as most of the fruits have
not yet traded. Research carried out by employing survey on the Sitoluama and
Simardangiang Villages in North Tapanuli Regency in 2015. Respondents (n=64) were
chosen purposively, based on gender and age classification. The results showed that
there were 28 kinds of fruits are utilized, which were categorized in to four utilization
categories, namely as a fresh fruit, processed fruit, spices and medicines. In Sitoulama
Village, five most important fruits based on LUVI are kapundung (0.50), hopong (0.41),
sihim (0.32), handis (0.32) and mobe (0.19). Meanwhile, in Simardangiang Village were
kapundung (0.56), hopong (0.52), sotul (0.48), harimonting (0.47) and habo (0.42).
Based on these findings, the fruit forests that have domestication potency are kapundung,
hopong, sihim, handis, mobe, harimonting, sotul and habo.
Keywords: forest fruits, kapundung, mobe, harimonting, LUVI
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
64
Probiotic Powder Production for Cattle by Using Response Surface
Methodology
Rohmatussolihat, Roni Ridwan, Nurul Fitri Sari, Rusli Fidriyanto, Wulansih Dwi Astuti,
Yantyati Widyastuti
Research Center for Biotechnology-Indonesian Institute of Sciences
Jl. Raya Bogor KM 46 Cibinong 16911
Abstract. At present, the use of antibiotics and growth hormones for livestock has been
prohibited by the regulations. Probiotics are one of the additives that are safe and
permitted by applicable regulations, and also can replace the use of antibiotics. Probiotics
are live microorganisms used in livestock to improve microbial balance and positive
effects on host animals. The purpose of this study is to optimize the production media of
probiotic powder using Central Composite Design-Response Surface Methodology
(CCD-RSM). The RSM-CCD is prepared using four variables, namely starch, CaCO3,
Corn Gluten Meal (CGM) and zeolite, with five level combinations. The drying process
of probiotic powder uses a vacuum oven at 35-450C for 20-30 hours, the vacuum process
is carried out using a vacuum pump for 1-3 hours. The probiotic survival amount is
measured by the total plate calculation method. Statistical analysis showed that the
viability of probiotics was significantly affected by CaCO3, CGM and zeolite. The
optimal starch concentration was 29.9%, CaCO3 44.42%, CGM 29.96%, zeolite 55.58%
with a maximum estimate of probiotic results surviving at 98.89%, which significantly
increased by 50.22% . These results indicate that the percentage of probiotic survival
increased by 40.97% when the optimal concentration of ingredients was used.
Key words: probiotic, production, cattle, response surface methodology
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
65
The Effects of Clove Oil On Coral, Trachyphyllia Geoffroy, Using
Biomarkers; Color Change of Coral, Density And Mitotic Index of
Zooxanthellae
Sri Wahyuni Rahim; Khusnul Yaqin; Hadiratul Kudsiah
Department of Fisheries, Faculty of Marine Science and Fisheries, Hasanuddin University,
Makassar, Indonesia
Correspondent email: [email protected]
Abstract. The use of clove oil as an alternative to environmentally friendly cyanide is one
form of sustainable use of coral reef ecosystem resources. However, it is necessary to
consider the quality of the post-capture coral before the application of clove oil. This
research aims to analyze the quality of Trachyphyllia geoffroy coral after clove oil
exposure. The experimental design used a factorial design, consisting of 7 variations of
clove oil concentration and 5 time variations with 3 repetitions. The coral exposed clove
oil with different concentrations (control, 20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm, 70
ppm) for 5 minutes. Subsequently transferred to the aquarium recovered for 60 seconds
then transferred to a tank for analysis of changes in coral for 17 days. The results showed
that the exposure of clove oil with a concentration of 20 - 70 ppm in corals did not cause
coral discoloration, there was no significant difference between the density and mitotic
index of the coral and variations in clove oil concentration after exposure (p> 0.05). This
shows that clove oil with a concentration of 20-70 ppm has not caused changes in color,
density and mitotic index of zooxanthellae in Trachyphyllia geoffroy corals.
Keywords: clove oil; mitotic index, Trachyphyllia geoffroy; zooxanthellae
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
66
Identification and Test of Fungi On Kaliandra (Calliandra calothyrsus) Seed
Which Is Potential As Antagonist Agent
Tati Suharti1*, Wildani Hanifah
1, Sri Listiyowati
2, YMM Anita Nugraheni
1, Danu
1
1Forest Tree Seed Technology Research and Development Center (BP2TPTH), Bogor 16001,
Indonesia 2Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University Bogor
16680, Indonesia
*Corresponding author : [email protected]
Abstract. Kaliandra (Calliandra calothyrsus) is an economic important plant and used as
a renewable energy source. Seed-borne fungi can be beneficial or detrimental. One of the
beneficial roles of fungi is as an antagonistic agent. Kaliandra seed are commonly
infected by pathogenic fungi such as Fusarium sp., Aspergillus sp. and Rhizopus sp. The
purpose of this research was to identify the fungi in Kaliandra seeds which is potential as
antagonistic agent and study their ability in inhibiting growth of pathogenic Fusarium sp.,
Aspergillus sp. and Rhizopus sp. The seeds obtained are from Papua. Fungi was isolated
by soaking the seeds with 1% NaOCl for 3 minutes then washed with sterile aquades, it
was repeated up to three times. Furthermore, the seeds are sown on moist paper media
and incubated until a fungus colony emerges which is expected to be a potential
antagonistic agent. The result showed that there was one isolate of fungi that was
potential as an antagonistic agent, it was Talaromyces sp. The inhibition percentages of
Talaromyce sp against Aspergillus sp., Fusarium sp. and Rhizopus sp. were 67.92%,
59.36%, 8,75%, respectively.
Keywords: antagonist agent, fungi, seed borne pathogen, Talaromyces sp.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
67
Potency of Indonesian Nutmeg Oil for Combating Halitosis
Yanti*, Ika Kristiyaningjati, and Bibiana Widiyati Lay
Faculty of Biotechnology, Atma Jaya Catholic University of
Indonesia, Jakarta 12930, Indonesia
*Corresponding author: [email protected]
Abstract. Halitosis is an oral health problem for people who has caries and periodontal
disease. About 85-90% of the causes of halitosis is bacteria that lives in the oral cavity.
Nutmeg seed (Myristica fragrans) is a spice originated from Banda Island, Moluccas
(Indonesia) that has been claimed to exert antibacterial activity against Gram-positive and
Gram-negative bacteria. In this study, we investigated whether essential oil extracted
from nutmeg seed at various doses (20-100 g/ml) had antihalitosis effect against
Streptococcus mutans by conducting biofilm, volatile sulphure compounds (VSCs), and
pH-stat assays. Essential oil from nutmeg seed was extracted using hexane and identified
for its major compounds by gas chromatography mass spectrophotometry (GC/MS).
Major compounds in nutmeg oil were myristicin, methoxyeugenol, and
dehydrodieugenol. Nutmeg oil dose dependently prevented the S. mutans biofilm
formation up to 60% and damaged the established biofilms up to 75%, indicating its
antibiofilm potency for preventing and eradicating the S. mutans biofilms. Nutmeg oil
also inhibited the VSCs production up to 71% and reduced total acids produced by S.
mutans in 20 minutes resulting in pH of 5.35 from the initial pH of 7.00. Therefore,
nutmeg oil could be considered as a potential nutraceutical for application of oral
healthcare products.
Keywords: antihalitosis activity, nutmeg oil, oral healthcare, Streptococcus mutans
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
68
The Prospect of Microorganism in the Silver Nanopaticle Biosynthesis to
Enhance Antibiotic Drug Activity as an Alternative Solution to Combat
Resistances
Mega Ferdina Warsito
Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Komplek
CSC-LIPI Jl. Raya Bogor Km 46, Cibinong 16911, West Java, Indonesia.
Corresponding author: [email protected]
Abstract. Antimicrobial resistance (AMR) has become major concern worldwide.
Nanotechnology become an alternate solution to combat these emerging problems, through
the formulation of antibiotic drugs using nanoparticle as the delivery system to enhance the
activity and reduce toxicity. Interaction between nanoparticle and bacteria can cause
membrance distruption and toxicity to pathogenic microorganism, thus the combination of
silver nanoparticle and antibiotic subtances hopefully can increase therapetic efficiency.
Several microorganism, such as actinomycetes, Eschericia coli, Pseudomonas sp,
Aspergillus flavus, Bacillus sp., Penicillum and Saccharomyces cerevicae have been
known to be able to synthesis silver nanoparticle. This is an alternative method to the
chemical and physical synthesis processes, which is more environmental friendly. The
biological synthesis process also considered easy and efficient compare to the conventional
one. Furthermore, the biogenic nanoparticle have been proved to have board spectrum
activity against gram negative and gram positive bacteria, including the pathogenic fungi.
This review will discussed the microbial agent that have been known for their silver
nanoparticle synthesis ability and their usage in the delivery system of antibiotic subtances.
Keywords : silver nanoparticle, microorganism, resistance, biosynthesis
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
69
Review: Fungal Glycoside Hydrolases of White-rot Fungi for Cellulosic
Biofuels Production
Sunardi1,4*
, Wiwin Tyas Istikowati2, Futhoshi Ishiguri
3, Shinso Yokota
3
1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Lambung
Mangkurat University, Banjarbaru 70714, Indonesia 2Faculty of Forestry, Lambung Mangkurat University, Banjarbaru 70714 Indonesia
3Faculty of Agriculture, Utsunomiya University, Utsunomiya, Tochigi 321-8505, Japan
4Wetland-Based Materials Research Group, Lambung Mangkurat University, Banjarbaru
70714, Indonesia
*Corresponding author: [email protected]; [email protected]
Abstract. The second generation bioethanol production from lignocellulose materials
through environmentally friendly methods is one of the biggest challenges on industrial
application. Enzymatic hydrolysis of cellulose has more benefits compared with the acid
hydrolysis This method has the good specificity, low consumption of energy and
chemicals, and is more environmentally friendly. However, the utilization of lignocellulose
for bioethanol production through enzymatic methods is still confronting several
difficulties for commercialization. Cellulose hydrolysis step has been reported to be the
bottleneck of bioethanol production by enzymatic process, and the major barrier of this
process is high price of enzymes, which making the process less economically feasible. For
this reason, many developments are still needed in cellulase production from various
organisms for cellulose saccharification. White-rot fungi have received much
consideration for their valuable enzyme systems which can effective degrade
lignocellulose biomass. These fungi could secrete extracellular oxidative and hydrolytic
enzymes that degrade lignin, hemicellulose, and cellulose. This review provide a
complete overview of the glycoside hydrolases enzymes production by white-rot fungus,
such as endoglucanase, exoglucanase, beta-glucosidase, cellobiose dehydrogenase and
lytic polysaccharide monooxygenase. The use white-rot fungus for low cost glycoside
hydrolases enzymes production might be fascinating for second generation bioethanol
production.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
70
Comparison of Dissolved Organic Carbon (DOC) and Physicochemical
Variables of Peat Water Among Three Tropical Peat Swamp Forests
Siti Sundari*
Research Center for Biology, Indonesian Institute of Sciences,
Cibinong Science Center, Cibinong-Bogor 16911, Indonesia
*Corresponding author: [email protected]
Abstract. Comparison of dissolved organic carbon (DOC) and physicochemical variables
of peat water had been conducted in three tropical peat swamp forests of Central
Kalimantan. They were pristine peat swamp forest in Sebangau (SF), burnt peat swamp
forest (KB) in Kalampangan, and drained peat swamp forest (KF) in Kalampangan with
dominant tree species were Combretocarpus rotundatus and Cratoxyllum arborescens.
This research aims to investigate effects of drainage and fire on DOC concentration and
physicochemical variables of peat water in the tropical peat swamp forests. The DOC
concentration was analysed by total organic carbon (TOC) analyzer, while
physicochemical variables including electrical conductivity (EC), pH, and ions were
analysed by EC meter, pH meter, and ion analyzer, respectively. Research results showed
that the highest DOC concentration was measured in KF, whereas the lowest DOC
concentration was in KB.
Keywords : DOC; drainage; fire; peat swamp forests; physicochemical variables
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
71
Agroforestry Practices In The Use of Dry Land In Gunungkidul District,
Yogyakarta, Indonesia
Sudomo A.1 dan Gerhard E. S.
2
1Research and Development Institute of Agroforestry Technology, Ciamis, West Jawa,
Indonesia 2World Agroforestry Center-ICRAF, Bogor, West Java, Indonesia
Email: [email protected]
Abstract. This research is aimed to improve the contribution of agroforestry for villagers
as the agro-ecosystem. The specific purpose is to find agroforestry practices that has been
applied by the local people to support food security on dry land utilization in Bejiharjo of
Karangmojo Sub-District of Gunungkidul District of Yogyakarta Province of Indonesia.
There were 90 plots and belong to 66 farmers/ household to be observed. Interview on land
owner has been conducted to find out the utilization of plant species and land cultivating
technique. The research showed that planting pattern on four types land utilizations (home
garden, farming land, area between farming land, and private forest) was tree mixed
garden, agrisilviculture, silvopasture, and monoculture. Types of applied agroforestry
pattern were alleycropping, border trees, and mix/ random. Types of dominant tree in local
people land is Tegtona grandis (70.75%), Swietenia mahagoni L (9,8%), Acacia mangium
(3,18%), Sesbania grandiflora (0.05%), Leucaena glauca (L) (1.78%), and Gliricidia
sepium (0.16%). The dominant types of fruit tree were Cocounut nucifera (3.18%),
Mangifera indica (1.8%), Syzygium sp (0.64%), Gnetum gnamon (1.6%), and Manilkara
kauki (0.21%). The dominant farming plants on land among farming area were corn,
peanuts, chili, and cassava. Types of trees that grow under canopy were Colocasia
esculanta, Maranta arundinacea, Dioscorea esculenta, and Canna edulis. Medicine trees
that grow under canopy were ginger, turmeric and Alpinia galanga. Animal feeds were
grass, leaves, and twigs. Agroforestry is subsistent and semi-commercial applied as a kind
of adaptation with bio-physical environment, limited land area (<0,5 ha/ household) and
food needs.
Keywords: Agroecosistem, agrosilviculture, dry land, food security, village.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
72
Irradiated Foxtail Millet (Setaria italica (L.) P.Beauv) : Agronomic And
Physiological Performance Under Low Light Intensity
Titi Juhaeti
Bidang Botani, Pusat Penelitian Biologi LIPI
Jl. Raya Jakarta Bogor km 46 Cibinong 16911 Indonesia
Corresponding author: [email protected]
Abstract. The research was carried out to study the agronomic and physiological
performance of irradiated foxtail millet (IFM) under low light intensity as an effort to
obtain shade tolerant foxtail millet. Experimental design was RCBD with two factors.
The first factors were the level of light intensity 0% and 50% shading intensity. The
second factors were IFM namely GM75.16, GM25.12, M2GM3, GM100.10, GM75.14,
GM100.20 and GM0.5 (control, without radiation). The observed variables included plant
growth, chlorophyll content (CC), photosynthesis rate (PR), and panicle production (PP).
The research showed that low light intensity decreased CC and PR value; thereby
reducing plant growth and production. The CC value in 50% shading (33.02 SPAD) was
significantly different with 0% shading (47.56 SPAD). The PR value at 50% shading
(7,112µmolm-2
s-1
) was significantly different from 0% shading (19,379µmolm-2
s-1
).
Observations on IFM showed that GM 75.16 potential as shade tolerant plant. The plant
height and the leaves number of GM 75.16 were not significantly different with control.
The GM75.16 (17.03g) showed the highest PP and significantly different with GM25.12,
M2GM3, GM100.10 and GM100.20 but not significantly different from control (16.24 g)
and GM75.14 (14.10g). It can be concluded that the decreased of CC and PR under low
light conditions resulting in decreased growth and crop production. Irradiated foxtail
millet GM75.16 was potential as shade tolerance foxtail millet.
Keywords: agronomic, foxtail millet, low-light intensity, physiological
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
73
Morfological Characterization of Local Durian from Banten Province
Zuraida Yursak, Ulima D Amanda, Dewi Widiyastuti, Pepi Nur Susilawati
Peneliti Balai Pengkajian Teknologi Pertanian Banten
Jl. Raya Ciptayasa KM.01 Ciruas Serang Banten 42182
Email : [email protected]
Abstract. Durian (Durio zibethinus) is an exotic fruit with good varietal improvement
prospects. Indonesia is one of the countries with high durian diversity, one of which is
located in Banten Province. This study aims to determine the diversity and relationships
of several local durians in Pandeglang and Lebak Regencies of Banten Province based on
the morphological characters of leaves, flowers and fruit. The durian samples were
obtained by purposive sampling and interview methods. The characterization was carried
out according to the guidelines of the "International Union for the Protection of New
Varieties of Plant" including the description of stems, leaves, flowers, fruits, and seeds.
After characterization, scoring, standardization, and similarity index (IS) were calculated
between samples. Cluster analysis using Cluster Observation with Minitab software. The
analysis was conducted on 97 characters in 14 local durian accessions. Dendogram
obtained shows 4 groupings in the Similarity Index of 35.19%: Cluster 1: Si Ceer, Si
Bintang, Si Radio, Si Jangkung; Cluster 2: Kempong, Grinsing, Kadu Katung, Si Cayut;
Cluster 3: Si Bening, Hauk, Kueh Kuning; Cluster 4: Kueh Hijau.
Keywords: Durio zibethinus, relationships, morphological characters
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
74
The Diversity Of Garlic Bulbs And Cloves Quantitative Character In Local
Garlic Collection Of East Java AIAT
Ratih Sandrakirana1*
, Maureen Chrisye Hadiatry1, and Baswarsiati
2
1,2
East Java Assessment Institute for Agricultural Technology (AIAT)
Jalan Raya Karangploso KM.4 Malang-East Java, Indonesia
*Corresponding author: [email protected]
Abstract. Since 2017, garlic has become one of the important commodities that received
special attention from government considering its imbalance conditions in the domestic
market of garlic. Based on Statistics Indonesia‟s data, domestic garlic production is only
able to meet 5% of national needs. For that purpose, the government has established a
self-sufficiency plan for garlic in 2045 through increased domestic production. However,
the limitation of clove quality and the limitation data of the character description for
garlic accessions has become a major constraint in itself in terms of the similarity of bulb
characters and cloves between accessions. AIAT has conducted an exploration of garlic
germplasms to several areas of production centers as a source of planting materials for
clove production. The objective of this study is to collect and to identify differentiated
characters from the garlic accessions. This activity took place from August 2017 to
March 2018 in Batu, Malang, Mojokerto, Pasuruan, Probolinggo and Temanggung
Regency. From the exploration activities obtained the results in the form of bulb of 4
varieties and 3 garlic accesions. Eight characters were observed, which is consist of bulb
weight character, bulb diameter, bulb length, clove weight, diameter of clove and cloves
length. In addition, there are two characters that are the ratio of the length/diameter of the
bulbs and cloves indicating the diversity between accessions and potentially to be used as
differentiator between accessions.
Keywords : Bulbs, accessions, cloves, exploration, character
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
75
Detection of Adulteration Activities in Edible Bird’s Nest Using NMR
Metabolomics
Yong Chin Hong1, Syahidah Akmal Muhammad
1,2,*, Fatimatuzzahra Abdul Aziz
3, Ng Jing
Sheng1, Fatin Ilyani Nasir
2
1 School of Industrial Technology, Universiti Sains Malaysia, 11800, USM, Penang, Malaysia
2 Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 USM, Penang,
Malaysia 3
School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, USM,
Penang, Malaysia
*Corresponding author: [email protected]
Abstract. Edible bird‟s nest (EBN) is the nest of swiftlet made from the saliva of male
swiftlet (Aerodramus or Collocalia species). Due to its nutritional values, EBN is
recognized as a premium food and highly in demand among Chinese community. EBN is
commonly adulterated with cheaper ingredients and efforts are being made to combat the
activities using different analytical techniques. Metabolomics is an approach to analyse the
metabolome, both small molecule intermediates and products of metabolism. Hence, in
this study, we employed nuclear magnetic resonance (NMR) metabolomic fingerprinting
to detect adulteration activities in EBNs. A sample preparation procedure was developed
for this purpose. Briefly, 35mg of EBN sample was heated in 1 mL distilled water for 30
minutes, followed by filtration. The filtrate was then mixed with pH 7 buffered solution
containing an internal standard before analysis with NMR. Once the sample preparation
was finalized, it was used to analyse more than 30 EBN samples for their metabolomic
fingerprints which were then subsequently profiled using chemometrics. Overall, this work
showed that the discrimination of authentic EBNs from the adulterated ones using NMR
metabolomics was successful and the technique promises to be an indispensable tool for
food authenticity work in the future.
Keywords : Edible bird's nest; Nuclear Magnetic Resonance, Metabolomics,
Adulteration, Food authenticity
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
76
Effect of Amphipilic Lignin Derivatives (A-LD) Surfactant Addition on the
Fermentation Process of Sorghum Bagasse Kraft Pulp for Bioethanol
Production
W Fatriasari1,*
, A Karimah2, F Falah
1, S H Anita
1
1Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI),
Jl. Raya Bogor Km. 46, Cibinong Science Center, Cibinong, Bogor 16911, Indonesia 2Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Gadjah Mada
University, Indonesia.
Email: [email protected]
Abstract. This research was intended to study the effect of Amphipilic Lignin Derivatives
(A-LD) surfactant addition on the bioethanol production by using simultaneous
saccharification and fermentation (SSF) system. A-LD synthetis was obtained from single
step isolation of lignin from black leachate as a by-product of the Acacia mangium
kraft process. This synthesis was carried out by reacting single step lignin with the
polyethylene glycol (PEG) which was oxidized at 60 ºC for 1 h. SSF in this study was
performed by using Saccharomyes cerevisiae InaCC Y93. Cellulase enzyme of 50 FPU/g,
10% and 20% yeast inoculum, and 15 mg/mL A-LD were added into 5.37 g (wet weight)
of sorghum bagasse kraft pulp. The concentration of reducing sugar and the ethanol
content were analysed every 24 h for 72 h and also at 89 h. The ethanol concentration,
ethanol yield, and the highest percentage of theoretical ethanol yield were 4.91 ± 0.10 g/L,
0.06 ± 0.001 g/g and 11.29 ± 0.22%, respectively. They were obtained by 20% of yeast
inoculum for 24 h fermentation on SSF system and addition of A-LD surfactant. The
addition of ALD on SSF process can increase the ethanol production and also shorten the
fermentation time.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
77
Traditional Beekeeping of Closed Nested Native Honeybees (Apis spp.)
From Four Islands of Indonesia
Sih Kahono1*
, Erniwati1, Djunijanti Peggie
1, & Eko Sulistyadi
1
1Research Center for Biology, Indonesian Institute of Sciences,
Cibinong Science Center, Cibinong-Bogor 16911, Indonesia
*Corresponding author: [email protected]; [email protected]
Abstract. Three species of closed nested native honeybees, Apis cerana, A. koschevnicovi,
and A. nigrocincta, are recorded in Indonesia. Except of A. cerana distributes widely, the
others are island and geographic endemics. The bees live in nature adapt and synchronized
to their local environmental conditions. Local people usually well evolve to conduct
traditional beekeeping, however a few of them have not been informed or published. We
conducted research on traditional beekeeping of four locations of Tasikmalaya (West
Java), Kayan Selatan (North Kalimantan), Peleng (Banggai Kepulauan) and Bawean (East
Java) islands. General activity of the traditional beekeeping was beginning by collection of
both honey of colonies from nature, then transferred the colonies into unframed wooden
boxes or holed dry trunks, and finally it settled at safety places close to the house. After
honey was harvested, a few of them keep stay in the boxes, however most of them escape to
the nature. This traditional beekeeping system follows natural phenology that have been
continued for long times.
Keywords: traditional beekeeping, Apis cerana, A. nigrocincta, A. koschevnicovi,
Indonesia
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
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78
Bioplastic from Modified Cassava Starch, Poly Vinyl Alcohol and Citric
Acid
Firda Aulya Syamani1*, Wida Banar Kusumaningrum, Fazhar Akbar and Bernadeta Ayu
Widyaningrum2
1Research Center for Biomaterials, Indonesian Institute of Sciences,
Cibinong Science Center, Cibinong-Bogor 16911, Indonesia
*Corresponding author: [email protected]
Abstract. Food packaging plastic from petroleum-based sources can be a problem to the
environment due to its non-biodegradability property. The development of bioplastic from
modified cassava starch will be an alternative to overcome that problem. Since
starch-based bioplastic, usually has low mechanical strength and damage if exposed to
water, it is necessary to modified with poly vinyl alcohol (PVA) and citric acid. Bioplastics
are made by heating a solution of modified cassava starch (cassava starch: distilled water =
1:20) at 75 °C for 1 hour on hot magnetic stirring plate. After starch solution was
gelatinized, glycerol (5% based on starch weight) was added, then PVA solution (1, 3, 5%
based on distilled water weight) was inserted gradually. The stirring was continued for 30
min. Afterward, citric acid (5% based on starch weight) was added into the solution and
stirring was continued for 15 min. The bioplastic solution is then poured into a (20x20) cm
acrylic sheet with a thickness of ± 5mm. Furthermore, it is cooled at room temperature for
3 days so that the bioplastic sheet is ready for mechanical strength testing based on ASTM
D 882-75b Tensile Properties of Thin Plastic Sheeting, and for thermal properties testing
with Thermo Gravimetry Analyzer.
Keywords : bioplastic; modified cassava starch; tensile properties; thermal properties
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
79
Mechanical Properties Investigation of Green Composite from Ramie
(Boehmeria nivea (L.) Gaud) and Epoxy
Ismadi1, Safira Vitasasti
2, Sukma Surya Kusumah
1
1 Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI), Bogor, Indonesia
2Match and Sciences Faculty of Padjadjaran University, Sumedang, Indonesia
Email : [email protected]
Abstract. In the last decade, requirement of environmentally friendly materials increased
significantly. Green composite materials were developed to provide the materials
requirement. One of green composite materials has been developed is natural fibre
reinforced epoxy composite. Ramie (Boehmeria nivea (L.) Gaud) fibre and bio-epoxy
were potential to arrange stronger and friendly composite. The paper present the
evaluation of rami fibre-epoxy composite in mechanical and physical characterization.
Rami fibre was arranged in one and three layers. The concentration of ramie fibre was
varied in 5, 7.5 and 10% of w/w composite weight. Hardener of epoxy matrix was
variated in 50 and 25% of w/w matrix weight. In composition of 50% hardener (w/w of
matrices), highest of Tensile Strength is 10,7 MPa. In 7,5% rami fibre content. Maximum
flexural strength was 34,5 MPa in 10% of rami fibre content. In the 25% of hardener
(w/w of matrices), the maximum tensile and flexural strength are 16,7 MPa and 33,57
MPa for 5% rami fibre content (w/w composites weight). In the three layers of fibre
mode, maximum strength of composite was 42,5 MPa for 10% of rami fibre content (w/w
of composite weight), and maximum flexural strength was 34,95 MPa for 7,5 % of rami
fibre content. In 25% hardener content, maximum of tensile strength was 45,8 MPa for
5% rami fibre content (w/w of composite weight), and maximum flexural strength was
31,35 for 10% rami fibre content. The lowest of water absorption of ramie-epoxy
composite was 6,75% for 7,5% of ramie fibre content in single layer mode. In the three
layer mode, the lowest water absorption was 6,72 % for 5% of rami fibre content.
Keywords: Green composite, tensile strength, flexural strength, ramie fiber, epoxy
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
80
Bioconversion of artemisinin by endophytic fungi associated Artemisia sp.
grown in Indonesia and Japan
Shoji Maehara1*, Ahmad Fathoni
2, Muhammad Ilyas
2, and Andria Agusta
2
1Faculty of pharmacy and pharmaceutical sciences,
Fukuyama University, 985, Sanzo, Higashimura-cho,
Fukuyama, Hiroshima, 729-0292, Japan 2Research Center for Biology, Indonesian Institute of Sciences,
Cibinong Science Center, Cibinong-Bogor 16911, Indonesia
*Corresponding author: [email protected]
Abstract. Artemisinin is a well-known anti-malarial drug that can be found in leaf of
Artemisia plant. The plant of Artemisia is growth widely in Europe, North America, North
Africa and Asia. Therefore, Artemisia plant that is called Wormwood or Mugword in
English, consist many plant species. The Indonesian mugwort or “baru cina”, and Japanese
mugwort or “Yomogi” has long been used as culinary herbs and medicinal plant. In this
report, we deal with symbiotic microorganisms in Artemisia plant grown in Indonesia and
Japan. Totally 15 endophytic fungi were isolated form Artemisia from Indonesia, 21
endophytic fungi from Artemisia from Japan. It was found that the composition of each
endophytic fungi varies depending on the growth environment (region). Isolated
endophytic fungi that promote the bioconversion reaction using artemisinin as a substrate
have been clarified by screening tests. Interestingly, it was found that the microbial
conversion reaction of each endophyte was different depending on the growth environment
(region). Here we show reactions by endophytic fungus DnAvCw-1 and Y13-2 associated
Artemisia plant grown in Indonesia and Japan. It was revealed that artemisinin was
deoxygenated by DnAvCw-1 and hydroxylated by Y13-2.
Keywords : artemisinin; endophytic fungi; bioconversion; anti-malaria; Artemisia
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
81
Larvicidal Activity of Cerbera odollam against Culex quinquefasciatus
(Diptera: Culicidae)
Didi Tarmadi1*
, Sulaeman Yusuf1, S. Khoirul Himmi
1, Maya Ismayati
1, Ikhsan Guswenrivo
1,
Titik Kartika, Dita Meisyara1
1Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor
KM. 46, Cibinong, Bogor 16911, Indonesia
*Corresponding author: [email protected]
Abstract. Mosquito is a major public health problem as vector of hazard human disease.
Culex quinquefasciatus, a vector of filariasis is widely distributed in tropical and
subtropical zones. In fact, filariasis is primarily an urban disease of the tropics and
hazardous disease. Plants can be used as an alternative source of mosquito larval control
materials to substitute chemical materials. This study aimed to examine larvicidal activity
of Cerbera odollam against a filariasis vector, C. quinquefasciatus. We applied
twenty-five 2nd instar of C. quinquefasciatus into the crude extract solutions derived
from rind, steam bark, leaf and seed kernel of C. odollam. In addition, the present study
also examined the larvicial activity from fractionated extracts derived from seed kernel of
C. odollam. The results showed that larvicidal activity was very low for crude extracts
from, rind, steam bark, and leaf, however, the present study detected moderate larvicidal
activity for seed kernel. The larvicidal activity of fractionated extract showed high larval
mortality in ethyl acetate, and moderate in n-hexane.
Keywords: larvicidal activity, Culex quinquefasciatus, natural extractive, filariasis
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
82
Conservation of Kelulut (stingless bees) in East and North Borneo,
Indonesia
Syafrizal1*, Irawan Wijaya Kusuma
2, Oshlifin Rucmana Saud
2, Restina Wiandany
2, and
Harmonis2
1 Faculty of Mathematic and Science, Mulawarman University, Samarinda, Indonesia
2 Faculty of Forestry, Mulawarman University, Samarinda, Indonesia
*Corresponding author: [email protected]
Abstract. Southeast Asia is home to at least 9 species of native honey bees, one of which is
Kelulut (stingless bees). The Kelulut is very valuable because it is a major pollinator for
tropical plants, provides significant income for some members of the local community, and
is an important component in the food network system. In addition, the Southeast Asian
Dipterocarp forests, particularly East and North Kalimantan, appear to be adapted to
pollination by honey bees. Current concerns about the decline of plant and pollinator
species that are directly related to ecosystem functions have focused on local and global
threats to bee diversity. Because evidence of bee decline is now accumulating on broad
taxonomic and geographical scales, we review the role of Kelulut cultivation based on
species and habitat approaches. Species diversity, population size, and growth rates are
influenced by temporal-spatial variations, all of which play an important role in their
vulnerability to extinction. As such, this will enhance our understanding of the factors and
processes that are behind bee decline, to ensure the long-term survival of the bees and the
important pollination services they provide.
Keywords : bee; borneo; cultivation; diversity; pollination
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
83
Allelopathy of Invasive Species Air Potato (Dioscorea bulbifera L.) and Its
Effect on Germination of Meranti (Shorea selanica (Lam.) Blume
Ade Ayu Oksari1,♥
, Irvan Fadli Wanda2,4
, Gladys Ayu Paramita Kusumah Wardhani3
Biology Department, Nusa Bangsa University, Jl. KH. Sholeh Iskandar Km 4, Bogor,
Indonesia. 161661*
Research Center for Plant Conservation and Botanic Gardens-Indonesian Institute of Sciences
(LIPI). Jl. Ir. H. Juanda No 13 Bogor, Jawa Barat, Indonesia2
Chemistry Department, Nusa Bangsa University, Jl. KH. Sholeh Iskandar Km 4, Bogor,
Indonesia. 161663
Biology Department, Faculty of Mathematics and Natural Sciences, IPB University, Bogor
16680420 09
20
*Corresponding author: [email protected].
18 (Date of abstract/manuscript submission). Revision accepted: .................... 2018.
Abstract. Dioscorea bulbifera L (Dioscoreaceae) is one of the most unutilized Invasive
plants.This Plant contains alelopathy that affects the physiological process of native
species. The study aims to know effect of allelopathy and type of Allelochemical of the
various concentrations leaf and tuber extracts of D. Bulbifera on seed germination of
Meranti (S. selanica (Lam.) Blume). The method used a Completely Randomized Design
with ten treatments and three replications. The treatment given the concentration of
extract D. bulbifera 25, 50, 75, 100%, and control. Based on the results of phytochemical
screening tests all secondary metabolites in positive leaf extract results. Saponin, tannin,
flavonoid, and steroid / terpenoid compounds are strongly detected, while alkaloid
compounds are less strong. In the tuber extract, compound compounds saponins,
flavonoids and steroids / terpenoids are detected strongly, while tannins are detected less
strongly. Alkaloid compounds are not contained in the tuber extract. The total phenolic in
leaf extract is 2.19 ± 0.13, the value is greater than the tuber extract of D.bulbifera which
is 0.96 ± 0.01. Phenolic compounds in leaf extracts consist of two types (phenol and
1,2-benzenediol). Tuber extract has 4 types of phenolic compounds. Two compounds of
the same type as phenolic compounds in leaf extracts (phenol and 1,2-benzendiol) while
the other compounds are hydroquinone (1,4-benzendiol) and 2-methoxyphenol. The
results showed that allelopathic substances and concentrations of D. bulbifera affected
the germination of S. selanica seeds. Based on DMRT test at 5% level, it was known that
allelopathy concentration of D. bulbifera significantly affected the germination capacity,
Coefficient Rate of Germination and Coefficient of Simultaneity of Germination with all
concentrations. The concentration and source of extracts that reduced the lowest seed
viability was at a concentration of 75% and 100 % with germination capacity 10.00% ±
6.32 in leaf extract and 0.00% ± 6.32 in tuber extract.
Keywords: allelopathy, D. bulbifera L., germination, S. selanica (Lam.) Blume.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
Toward 2025 Sustainable use of Biodiversity
84
Morphological Characteristic of Apple Velvet (Dispyros blancoi) at
Germplasms Garden of LIPI Cibinong Collection
Puspita Deswina1*
, Novia Delliasari Aliyya Az Zahra2, Etty Hesthiati
2
1Research Center for Biotechnology, LIPI
2Agrotechnology Study Program, Agrotechnology Specialization Program, Faculty of
Agriculture, Universitas Nasional, Jakarta
*Contact: [email protected]
Abstract. Apple velvet (Diospyros blancoi) is one of the local fruits which is classified as
a rare plant with high economic value. Apple velvet has various morphologies that
require selection of various characters in order to identify the characters that are superior
and have high potential to be developed. Analysis of diversity of a population can be
identified to determine the morphological character of the plants. The notable
characteristics may include tree trunks, leaves, flowers, fruits and seeds. A study using the
quantitative and qualitative methods conducted in Germplasms Collection Garden of LIPI
Cibinong have shown that the apple velvet plants has the average height between 3.5-15
m, with stem, diameter between 14.1-72.29 cm. It has no distinctive shape of leaves such
as oval and wide oval. It has white flower crown, two shapes of fruits such as round and
oval pattern, and two shapes of seeds such as elliptic and biconvex.
Keywords: apple velvet plants, characterization, germplasm garden, rare plant
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
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85
Bee Pollen from Stingless Bee (Heterotrigona itama) as anti Acne*
Enos Tangke Arung1, 4**
, Irawan Wijaya Kusuma1, 4
, Ayu Mitha Sari 1, Yadi
2,4 , Sukemi
3,
Nataniel Tandirogang2,4
1. Lab. Forest Product Chemistry, Faculty of Forestry, Mulawarman University, Samarinda
2. Faculty of Medicine, Mulawarman University, Samarinda
3. Faculty of Education, Mulawarman University, Samarinda,
4. Research Center for Medicine and Cosmetics from Tropical Rainforest Resources,
Mulawarman University, Samarinda,
* This research funded by
Kemenristekdikti (No. 198/UN17.41/KL/2019)
**email: [email protected]
Abstract. In effort to search from natural resources for medicinal and cosmetic purposes.
Natural resources from tropical rain forests, especially non-timber forest products,
recently get attention such as honey, propolis and bee pollen. The stingless bee,
Heterotrigona itama produced honey, propolis and bee pollen. In this study we focused
on bee pollen ethanol extract for inhibition of Propionibacterium acnes. The diffusion
method was used and the results showed the ethanol extract of bee pollen inhibit the
growth of P. acnes. Based on these results, this extracts have the potential inhibition and
potential to be developed as a source of raw material for anti acne but certainly needs
some furthers experiment.
Keywords : Stinglees bee, bee polllen, Heterotrigona itama, Propionibacterium acnes
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
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86
"How Fisheries Technology Affect Production?" (Case Study: North
Gorontalo Regency)
Tikkyrino Kuriawan1 and Hakim Miftahul Huda
1
1 Research Centre for Marine and Fisheries Socio-Economics, North Jakarta, Indonesia
Abstract. Prohibition of several fishing gears has been started from 2015 by issuing
MMAF Ministry regulation no. 2 of 2015. The regulation prohibits several fishing gears;
one of them is seine nets. MMAF Ministry regulation no. 71 of 2016 details the size of
seine nets, including purse seine, which is not allowed. Fishers still believe that fishing
using purse seine with a large fleet is more profitable compared to hand line fishing gear
with a small fleet. The purpose of this study is to see whether the handline fishing gear is
a more profitable handline compared to purse seine. This study takes STBLK data from
PPN Gentuma and PPN Kwandang in the North Gorontalo Regency. The results show
that handline fishing gear is more profitable even with small boat sizes compared to large
vessels with purse seine fishing gear.
Keywords: Fishing Gear, Handline, Purse Seine, Ministry Regulation, MMAF
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
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87
The Effect of Sucrose Concentrations and Shoot Inoculum Density on The
Growth of In Vitro Shoot of Teak (Tectona Grandis l.) **
Witjaksono*, Indira Riastiwi, Katarina Utami Nugraheni and Gut Windarsih1
Plant Cell Tissue Culture Laboratory, Botany Devision, Research Center for Biology,
Indonesian Institute of Sciences, Jl. Raya Bogor Jakarta Km 46, Cibinong, Bogor 16911 1Universitas Islam Negeri Sultan Maulana Hasanuddin,
Jl. Syech Nawawi Al Bantani Andamui, Curug, Serang 42116, Banten
*Corresponding author: [email protected]; [email protected]
**All authors contributed equally to this work
Abstract. The teak belongs to the highly prized timber tree. Quality teak trees have been
guaranteed by planting clonal planting materials propagated vegetatively, for example by
shoot tip cutting and tissue culture. Tissue culture propagation of teak has been
commercially viable, eventhough efficiency of production can still be improved. This
research aims to study the effect of increasing shoot inoculum density and sucrose
concentration requirement for optimum growth of teak shoot in vitro. Inocula of a single
nodal shoot in the number of 5 or 9 were inoculated on modified MS basal media with
sucrose concentrations treatment of 20, 30, 40, 50 and 60 g l-1. The results showed that
standard sucrose concentration of 30 g l-1 was optimum for the growth five inocula,
while for nine inocula, the optimum sucrose concentration was 40 g l-1. Eventhough the
average shoot growth was lower for nine inocula than that of five inocula, the total shoot
growth was higher for the former. Increasing number of inocula can be used as a method
to increase the efficiency of shoot proliferation.
Keywords: teak, micropropagation, in vitro, sucrose, inoculum density, shoot culture
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
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88
Compression Strength of 9 Years-old Platinum Teak Wood
Mohamad Gopar1, Dimas Triwibowo
1, Fazhar Akbar
1, Wahyu Dwianto
1*, Akihisa Kitamori
2
and Takuro Mori3
1Research Center for Biomaterials, Indonesian Institute of Sciences,
Cibinong Science Center, Cibinong-Bogor 16911, Indonesia 2Research Institute for Sustainable Humanosphere, Kyoto University
Gokasho, Uji, Kyoto 611-0011, Japan 3Graduate School of Engineering, Hiroshima University
Kagamiyama 1-4-1, Hiroshima 739-8527, Japan
*Corresponding author: [email protected]
Abstract. Previous results on bending strength tests indicated that the 9 years-old Platinum
Teak wood could be classified into Strength Class II according to Indonesian Forestry
Vademecum Classification. Therefore, further mechanical strength tests are necessary to
recommend the wood for timber frame structures. Small clear specimens with a size of 40
mm in length x 20 mm in width x 20 mm in thickness were taken from the bottom, middle
and upper parts of 10 m stem in length of 9 years-old trees. Compression strength parallel
to the grain was tested according to British Standard BS 373-1957. The results showed that
the average oven-dried density of the bottom, middle and upper parts of stem were 0.61,
0.59, and 0.57 g/cm3, respectively. Compression strength values were slightly decreased
from bark to the pitch of wood. However, the average of compression strength values of the
bottom, middle and upper parts of stem were almost unchanged, i.e. 79.99, 75.85, and
75.71 N/mm2, respectively. According to the Classification, the wood could be classified
into Strength Class I, where the compression strength values were over 650 kg/cm2. These
results informed that the wood could be a quite suitable material for timber frame structures
of wooden construction.
Keywords : compression strength; Platinum Teak wood; timber frame structures
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
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89
Anti-tubercular Activity of Natural Product-derived Compounds (+)-1,1’
Bislunatin and (+)-2,2’-Epicytoskyrin A.
Listiana Oktavia1*, Vagolu Siva Krishna
2, Estharla Madhu Rekha
2, Ahmad Fathoni
1, Nikhila
Meda2, Andria Agusta
1, Dharmarajan Sriram
2
1Research Center for Biology, Indonesian Institute of Sciences,
Cibinong Science Center, Cibinong-Bogor 16911, Indonesia 2Department of Pharmacy, Birla Institute of Technology
and Science-Pilani, Hyderabad Campus, Jawahar Nagar,
Hyderabad 500078, India
*Corresponding author: gmail.com /[email protected]
Abstract. Tuberculosis (Tb) still remains a great health concern as it was estimated in
2016, 1.7 million deaths because of this disease. Current drug development pipeline is still
insufficient to completely eradicate the causative bacteria from Tb patients as recently it
was found that the occurrence of multi drug-resistant Mycobacterium tuberculosis (Mtb).
Therefore, there remains an essential need to discover and develop a novel and effective
anti-tubercular drug. In this study, two secondary metabolites isolated from endophytic
fungi Diaporthe sp. GNBP-10 derived from a gambier plant Uncaria gambier Roxb.
(+)-1,1‟-Bislunatin (Bis) and (+)-2,2‟-Epicytoskyrin A (Epi) were evaluated as
anti-tubercular agent by determination of Minimum Inhibitory Concentration (MIC)
against M. tuberculosis H37Rv. The in vitro assays for Mtb in the dormant and biofilm
mode were also conducted. The Cytotoxicity was evaluated by MTT assay in raw 264.7
macrophages normal cell. Furthermore, the ability of eradicate Mtb inside the host cell
(macrophages) was also investigated. For further evaluation, the docking study was
conducted as a preliminary study to understand the interaction between tested drugs and
potential protein target. Epi and Bis show a promising anti-tubercular activity as shown
by MIC value 0.422 µM and 0.844 µM respectively. Moreover, Epi and Bis also was
observed for having the ability to combat dormant and biofilm of Mtb model with relatively
moderate bacterial reduction in both model with log reduction between 1-2 fold. Epi shows
4% cell inhibition at the lowest tested concentration (0.01µM), while Bis remains toxic in
all tested concentrations (70%). Both compounds can penetrate through macrophages and
active to reduce the number of Mtb in 2 fold log reduction. The docking result shows that
both compound has good affinity with pantothenate kinase (PaNK) enzyme with glide
score -8.427 kcal/mol and -7.481 kcal/mol for Epi and Bis respectively.
Keywords : anti-tubercular, molecular docking, multi drug resistance, natural product, ,
pantothenate kinase
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
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90
Growth and Development of Nine Accessions of Mutant Grass Plants
(Pennisetum purpureum cv. Taiwan) in the Field of Induction Mutation of
Gamma Ray
Husni, A*1
., Maftuha2, A.A. Fatmawaty
2, N. Hermita
2, and M. Kosmiatin
1 1Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and
Development (ICABIOGRAD), Bogor, 1611 Indonesia 2 Sultan Ageng Tirtayasa University, Serang-Banten, 42124 Indonesia
Abstract. The purpose of this research was to know putatife mutant accession Elephant
Grass cv. Taiwan is the best in growth and development as well as the plant on the 3rd
vegetative in the field. The design used in this research is completely randomized design
one factor of 9 the treatments putative mutan accession (76, 77, 78, 118, 120, 121, 125,
129, 130) and control (139). Research result showed that the treatment in the timing
of shoots appearing at accession 77 and 125 (3.753 and 3.742 day after planting), the
largest diameter of the stem at accession 139 (2.17 cm) while the smallest trunk diameter
is found in all putative mutant accessions, the highest plants at accessions 78 and 121
(112.45 cm and 100.53 cm), the highest number of tillers at accessions 76, 77, 78, 120,
121, 129, and 130 (25.16; 18.82; 24.67; 18.67; 24.83; 25.36; and 24.51), the highest
number of leaves/plants at accessions 139 (13.43 strands), the highest number of
leaves/clumps at accessions 121 (226.70 strands), the highest number of noods/plants at
accession 139 (8.69 noods), widest flag leaf at 139 (135.82 cm2), The fastest time
appears flower (66.5-78.75 day after planting) and the highest number of flowers was
found in all putative mutant accessions (1.05-2.03 flowers).
Keywords : Accessions, development, growth, mutation, mutant grass
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
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91
Rheological and Thermal Behavior of Polypropylene–Kenaf Pulp
Composite at Different Fiber and Compatibilizing Agent Loading
Nanang Masruchin1*, Wida Banar Kusumaningrum
1, Sudarmanto
1, Sasa Sofyan Munawar
1,
Ismadi1, Putri Amanda
1, Resti Marlina
1, Lisman Suryanegara
1, Subyakto
1, Bambang
Subiyanto1 and Arif Nuryawan
2
1Research Center for Biomaterials, Indonesian Institute of Sciences,
Cibinong Science Center, Cibinong-Bogor 16911, Indonesia 2Department of Forest Products Technology, Faculty of Forestry, Universitas Sumatera Utara,
Jl. Tri Dharma Ujung No.1 Kampus USU Medan, North Sumatera Indonesia 20155
*Corresponding author: [email protected]
Abstract. Our previous study reported that Kenaf pulp fiber have higher thermal stability
compared to that of Coconut and Pineapple pulp fiber. Therefore, in this study, we
proposed the manufacturing of polypropylene (PP) natural fiber composite using Kenaf
pulp fiber as reinforcing agents. Good fiber dispersion and better thermal stability are
necessary needed on the development of green polymer matrix composite. The rheological
of polymer composite compound was studied by the torque obtained during the mixing
process between melting polymer and Kenaf pulp fiber. Further, thermal degradation of
polymer composite at different Kenaf and compatibilizing agent (maleic anhydride
polypropylene, MAPP) loading were observed using thermogravimetry analysis (TGA).
Result showed that as Kenaf pulp increased the torque slightly increased. On the other
hand, the MAPP addition decreased the obtained torque for all fiber loading. Those
indicated the polymer chain mobility improved resulted in better Kenaf fiber dispersion.
After polymer and Kenaf pulp fiber was mixed and pelletized, those pellet was grinded and
analyzed using TGA. The energy activation and thermal stability of polymer composites
are undergoing investigation
Keywords : energy activation, green composite, Kenaf, polypropylene, TGA
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
Biodiversity, Genetic Resources And Innovative Bioresource Technology:
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92
Concoctions Developed from Correlations of Meliponicultured Honey with
Ethnoentomology in Langkawi, Malaysia
Abu Hassan Jalil1*, Mohd Razif Mamat
2, Azira Muhamad
2 and Nor Azfa Johari
2
1Akademi Kelulut Malaysia Sdn Bhd, 2625, Persiaran Rahmat, Teras Jernang, Bangi 43650,
Selangor, Malaysia 2Malaysia Genome Institute Jalan Bangi, Kajang 43000, Selangor, Malaysia
*Corresponding author: [email protected]
Abstract. The villagers of a 7-acre Meliponary in Northeast Langkawi, implemented
with an integrated farming system, have been using Honey mixed with popular herbs in
their traditional practices. Some of the local herbs include sea-cucumbers (gamat),
„Pepper Elder‟ (Peperomia pellucida), garlic, ginger, lemon and a mixture of honey with
'Tongkat Ali' (Eurycoma longifolia Jack). Some of the traditional practices that use Apis
honey by the villagers are replicated with stingless bee honey. Indigenous knowledge
traditionally uses Tongkat Ali's sweet honey (finely ground Eurycoma longifolia Jack and
macerated in honey) for the goal of manhood (erectile dysfunction ailments). Garlic
honey where garlic is burned and soaked in honey for two weeks, is good for prolonged
fever problems. The aim is profiling the metabolites in each of the concoctions/macerated
mixture of honey with four different local herbs. This profile will include the sugar,
amino acid and organic acid contents identified through metabolomics approach using
Nuclear Magnetic Resonance (NMR) spectrometer and Gas Chromatography Mass
Spectrometer (GCMS). From this data we will analyse and distinguish the active
compounds that are beneficial in the macerated mixture. We will ascertain the antioxidant
properties of the compounds identified. Cytotoxicity study using mammalian cell culture
will also be carried out. The resultant data will be expected to support the indigenous
knowledge in traditional treatment by providing scientific evidence to these practices.
Keywords: antioxidant properties; beekeeping; Cytotoxicity; Eurycoma longifolia Jack;
Garlic; Peperomia pellucida; sea-cucumbers; stingless bee honey
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
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93
Carbon Dioxide Absorption and Physiological Characteristics of Selected Tropical
Lowland Tree Species for Revegetation
La Ode Muhammad Muchdar Davis1, Nuril Hidayati
1*
1Research Center for Biology - Indonesian Institute of Sciences
Cibinong Science Center, Jl. Jakarta-Bogor Km 46 Cibinong 16911
*Corresponding author: [email protected]
Abstract
Biological diversity can make a significant contribution to reducing greenhouse gases in
the atmosphere. Trees form an essential part in the functioning of the terrestrial biosphere,
especially in the carbon cycle. Yet tree photosynthesis is far less studied than crop
photosynthesis. This research aims to assess the contribution of biological diversity in the
absorption of carbon dioxide (CO2) from the atmosphere by analyzing the physiological
characteristics that are related to CO2 absorption such as photosynthesis, transpiration,
stomatal conductance, leaf chlorophyll content of tree species for revegetation. The study was
conducted on selected tropical lowland trees curated at Cibinong Science Center-Botanical
Garden (CSC-BG), Indonesia. CO2 absorption, stomatal conductance, and transpiration were
measured by using an infrared gas analyzer (IRGA) photosynthesis apparatus, while
chlorophyll content was estimated using a SPAD chlorophyll-meter. Variation of CO2
absorption rate ranged from 3,42 to 20 µmolm-2
s-1
, with the highest rate was observed in
Teijsmaniadendron bogoriense, followed by Tectona grandis (19,67 µmol m-2
s-1
). Rate of
transpiration ranged from 4,7 µmol m-2
s-1
to 7,82 µmol m-2
s-1
, with that of Diospyros
blancoi being the highest, followed by Tectona grandis (7,65 µmol m-2
s-1
). CO2 absorption
and rate of transpiration are positively correlated to stomatal conductance and leaf chlorophyll
content. The stomatal conductance ranged from 0,19 µmol m-2
s-1
to 0,54 µmol m-2
s-1
,
with that of Tectona grandis being the highest, followed by Erythrina crista-galli (0,53 µmol
m-2
s-1
). Whereas the variation in leaf chlorophyll content ranged from 31 up to 78,43 SPAD.
Key Words: Biological diversity, tropical lowland trees, CO2, absorption.
The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium
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Important Date:
Full Paper Submission Deadline : October 25th, 2019
Registration and Publication of Symposium Papers:
The official language of the symposium is in English. Full-paper should be written in English. Please
send your full-paper to [email protected]