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Page 1: MANUSCRIPT PREPARATION GUIDELINES - LIPI
Page 2: MANUSCRIPT PREPARATION GUIDELINES - LIPI
Page 3: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

BSD City – Tangerang, Indonesia – 24th October, 2019

i

Background

Southeast Asia is one of biodiversity hot spots in the world. For example, Indonesia is

one of the 17 megadiverse countries and with 2 of the world‟s 25 biodiversity hotspots. Wide

variation of climate and numerous islands in the region also increased the biodiversity of the

region. This high biodiversity can potentially provide rich bioresources and genetic resources.

These biodiversity resources are expected to contribute to the sustainable development of the

region.

JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) was launched in 2015

to develop a platform of three fields, energy and environment, bioresources and biodiversity

and disaster prevention, for Japan-ASEAN science and technology cooperation to achieve

sustainable development in collaboration with various stakeholders in ASEAN and Japan.

Page 4: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

BSD City – Tangerang, Indonesia – 24th October, 2019

ii

Organizing Committee:

General Coordinator : Dr. Dede Heri Yuli Yanto

Dr. Joeni Setijo Rahajoe

Prof. Mamoru Kanzaki

Mr. Ryuichi Fukuhara

Advisory Board : Prof. Dr. Sulaeman Yusuf

Prof. Dr. Gono Semiadi

Prof. Dr. Andria Agusta

Prof. Takashi Watanabe

Dr. Yukako Monda

Dr. Ruliyana Susanti

Technical Committee Chairman : Dr. Didi Tarmadi Secretariat Dita Meisyara, S. Si

Riska Surya Ningrum, M. Sc

Anggi Permatasari, S.Si

Program : Dr. Sukma Surya Kusumah

Dr. Khoirul Himmi Setiawan

Dr. Ikhsan Guswenrivo

Dr. Maya Ismayati

Evana, M.Sc

Bernadeta Ayu Wdyaningrum, M.Si

Kartika Dyah Palupi, M.Med.Sc

Treasury and meal : Dwi Ajias P, M. Si

Listiana Oktavia, M. Si

Logistic and

accommodation : Yusup Amin, M.Si

Anugrah Fajar, S. T

Ahmad Fathoni, M. Si

Website : Ikut Tri Handoyo, M.Kom

Eko Widodo, S.T

Page 5: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

BSD City – Tangerang, Indonesia – 24th October, 2019

iii

Agenda

Time Agenda Speakers Moderator

(PIC)

07.00-8.00 Registration MC

OPENING CEREMONY

08:10-08:20 Welcome Remarks-1 Prof. Dr. Enny Sudarmonowati

Deputy of Life Science, LIPI

08:20-08:30 Welcome Remarks-2 Ms. Emi Kaneko

Japan Science and Technology Agency

(JST)

08:30-08.40 Welcome Remarks-3 Prof. Yasuyuki Kono

Leader of Japan-ASEAN Scientific,

Technology and Innovation Platform

(JASTIP) and Vice President, Kyoto

University

KEYNOTE SPEAKER

08.40-09.10 Dr. Ir. Arifin Rudianto, M.Sc

Deputy for Maritime Affairs and

Natural Resources, BAPPENAS

Prof. Takashi

Watanabe

09.10-09.40 IPBES Assessments on

Bioodiversity and Ecosystem

serveces and Toword Post

2020 Framework and

Achievement of SDGs

Dr. Noriaki Sakaguchi

Senior Advisor, Global Environment

Department, Japan International

Cooperation Agency (JICA)

09.40-09.55 Discussion

09.55-10.00 Photo Session

10.00-10.30 Break and Poster Session

SESSION I - Bioresources and Biodiversity

10.30-10.50 Invited speaker

“Emerging threats of invasive

ants to native pollinator

community: insight into virus

spillover”

Dr. Lin Chun-Yi

Kyoto University

Dr. Ikhsan

Guswenrivo

10.50-11.00 Discussion

11.00-11.10 Oral presentation 1

“Honey, a persisting NTFP on

a deforested peat landscape of eastern Sumatra”

Dr. Ahmad Muhammad

Riau University

11.10-11.20 Oral presentation 2

“A Preliminary Study on The

Population and Habitat of

Saltwater Crocodile

Ms. Grace Serepina Saragih

Research and Development Center for

Environmental Quality and Laboratory

Page 6: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

BSD City – Tangerang, Indonesia – 24th October, 2019

iv

(Crocodylus porosus) in Timor Island, East Nusa Tenggara”

11.20-11.30 Oral presentation 3

“High Corolla Colour

Variation of Hoya coronaria in

Belitung Island: Potential Use

and Conservation”

Dr. Sri Rahayu

Bogor Botanical Garden-LIPI

11.30-11.40 Oral presentation 4

“Exploration of Indonesian

Nutmeg Seed and Its Active

Compound for Management of

Arthritis Inflammation”

Dr. Yanti

Atma Jaya Catholic University of

Indonesia

11.40-11.50 Oral presentation 5

“Health Percentage of Coral

Reef In Tunda island, Banten Province”

Dr. Mujiyanto

Research Institute for Fisheries

Enhancement

11.50-12.00 Oral presentation 6

“Development of Bamboo

Propagation for Source

Material of Bamboo-based

Activated Carbon”

Dr. Riksfardini Ermawar

Research Center for Biomaterials, LIPI

12.00-13.00 Lunch / Poster session

SESSION II - Bioresources Utilization and Technology

13.00-13.20 Invited speaker

“Biocomposites from Natural

Fibers”

Prof. Subyakto

Research Center for Biomaterials, LIPI

Prof. Dr. Kenji

Umemura

13.20-13.30 Discussion

13.30-13.40 Oral presentation 7

“Wood Quality of

Kelempayan and Benuang

from East Kalimantan,

Indonesia: Fiber Quality and

Juvenile-Mature Boundary”

Ms. Sari Delviana Marbun

IPB University

13.40-13.50 Oral presentation 8

“The Effects of FeCl3

Concentration on Hydrothermal Pretreatment of

Oil Palm Fronds to Enhance

Reducing Sugar Production”

Dr. Sunardi

Lambung Mangkurat University

13.50-14.00 Oral presentation 9

“The Potential for Using Corn

Husk as Agricultural Waste for

Particleboard Raw Material”

Mr. Kurnia Widji Prasetiyo

Research Center for Biomaterials, LIPI

14.00-14.10 Oral presentation 10

“The Effect Application of

Organic Fertilizer and

Dolomit Toward Cassava

Production In Banten Province-Indonesia”

Dr. Viktor Siagiaan

Assessment Institute for Agricultural

Technology of Banten

Page 7: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

BSD City – Tangerang, Indonesia – 24th October, 2019

v

SESSION III - Bioprospecting and Sustainable Use of Bioresources

14.10-14.30 Invited speaker

“Balancing economic

development and biodiversity

protection – Biodiversity value

chains & certification of

products ”

Mr. Elpidio V. Peria

ASEAN Centre for Biodiversity

Prof. Hiroshi

Kamitakahara

14.30-14.40 Discussion

14.40-14.50 Oral presentation 11

“Bioconversion of artemisinin

by endophytic fungi associated

Artemisia sp. grown in

Indonesia and Japan”

Dr. Shoji Maehara

Fukuyama University

14.50-15.00 Oral presentation 12

“Isolation of Bioactive

Compounds from hyptis suaveolens (l.) Poit and Its

Antioxidant Activity”

Dr. Swe Swe Aye

Yangon University, Myanmar

15.00-15.10 Oral presentation 13

“Cosmeceutical Potency of

Functional Ripe Cider Buni”

Ms. Sthefanie Marchellia

Atma Jaya Catholic University of

Indonesia

15.10-15.30 Break/poster session

SESSION IV - Access and Benefit sharing: Lesson learnt from ASEAN countries

15.30-15.50 Invited speaker

ABS: Experiences and lessons

learned from Sarawak

Ms Margarita Naming

Senior Research Officer, Sarawak

Biodiversity Centre, Malaysia

Dr. Ruliyana

Susanti

15.50-16.10 Invited speaker Access and benefit sharing:

lesson learnt from Mycotech

Mr. Erlambang Ajidarma

Mycotech Indonesia

16.10-16.20 Discussion

CLOSING CEREMONY

16.20-16.25 Closing remarks Mr. Takeshi Miyazawa

First Secretary, Japan

Representative to ASEAN

16.25-16.30 Closing remarks Dr. Iman Hidayat

Director of Research Center for

Biomaterials, LIPI

.

Page 8: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

BSD City – Tangerang, Indonesia – 24th October, 2019

vi

Foreword

Good Morning,

On behalf of the organizing committee, I would like to say welcome to all

participants, especially Dr. Ir. Arifin Rudianto, M.Sc- Deputy for Maritime Affairs and

Natural Resources, BAPPENAS (National Plan/Policy on Bioresources-based Sustainable

Development) and Dr. Noriaki Sakaguchi, Senior Advisor, Global Environment Department,

Japan International Cooperation Agency (JICA) as Keynote speakers, and also Dr. Lin Chun

Yi- Kyoto University; Prof. Subyakto-Research Center for Biomaterials- Indonesian Institute

of Sciences (LIPI); Mr. Elpidio V. Peria- ASEAN Centre for Biodiversity; Ms Margarita

Naming, Senior Research Officer, Sarawak Biodiversity Centre, Malaysia; and Mr.

Erlambang Ajidarma, Mycotech Indonesia as Invited speakers. I also would like thanks Prof.

Dr. Enny Sudarmonowati-Deputy of Life Science- Indonesian Institute of Sciences (LIPI); Ms.

Emi Kaneko Japan Science and Technology Agency (JST); Prof. Yasuyuki Kono Leader of

Japan-ASEAN Scientific, Technology and Innovation Platform (JASTIP) and Vice President-

Kyoto University; and Mr. Takeshi Miyazawa, First Secretary, Japan Representative to

ASEAN.

7th JASTIP Symposium is organized by the JASTIP (Japan-ASEAN Science,

Technology and Innovation Platform), Kyoto University, Research Center for Biomaterials -

LIPI, Research Center for Biology-LIPI. The theme of this year is “Biodiversity, Genetic

Resources and Innovative Bioresource Technology: Toward 2025 Biodiversity sustainable

use.” This symposium is attended by 200 participants from some countries, i.e: Japan,

Malaysia, Myanmar, Philippines, and Indonesia. We received more than 70 abstracts for both

oral and poster presentations that vary in the broad scope of bioresources and biodiversity,

bioresources utilization and technology, and bioprospecting. The selected papers will be

published in IOP Conference Series indexed by Scopus.

I would like to express my deepest gratitude and sincere appreciation to all speakers,

presenters, and participants for their valuable contributions. I wish to express my special

thanks to all committee members who have spent significant time for the sake of the

conference successful. Hopefully, we will have a fruitful and memorable meeting in the

symposium, and also we can strengthen and develop our collaboration.

Thank you very much.

Prof. Mamoru Kanzaki

On behalf of Organizing Committee

Kyoto University

October 21, 2019

Page 9: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

BSD City – Tangerang, Indonesia – 24th October, 2019

vii

Table of Contents

Background ........................................................................................................................................ i

Organizing Committee ....................................................................................................................... ii

Agenda ............................................................................................................................................. iii

Foreword ...........................................................................................................................................vi

Table of Contents ..............................................................................................................................vii

KEYNOTE SPEAKER ....................................................................................................................... 1

Curriculum Vitae: Dr. Ir. Arifin Rudianto, M. Sc ............................................................................. 2

IPBES Assessments on Bioodiversity and Ecosystem serveces and Toword Post 2020

Framework and Achievement of SDGs Noriaki Sakaguchi........................................................... 5

INVITED SPEAKER .......................................................................................................................... 6

Emerging threats of invasive ants to native pollinator community: insights into virus spillover

Chun-Yi Lin and Chin-Cheng Yang ................................................................................................... 8

Biocomposites from Natural Fibers Subyakto, Nanang Masruchin and Sukma Surya

Kusumah .......................................................................................................................................... 11

Balancing economic development and biodiversity protection – Biodiversity value chains &

certification of products Peria, Elpidio; Schlegel, Bianca; Dr. Simorangkir; Dicky .................... 13

Successful Experience and Gaps in Access and Benefit Sharing (ABS) through Research on

Traditional Knowledge in Sarawak, Malaysia Margarita Naming and Yeo Tiong Chia .............. 16

Mycelium-Composite as Future Biomaterial Erlambang Ajidarma ............................................ 18

ORAL PRESENTATION .................................................................................................................. 19

A Preliminary Study on The Population and Habitat of Saltwater Crocodile (Crocodylus

porosus) in Timor Island, East Nusa Tenggara Grace Serepina Saragih, Kayat, Muhammad

Hidayatullah , Dani Sulistyo Hadi .................................................................................................... 21

High Corolla Colour Variation of Hoya coronaria in Belitung Island: Potential Use and

Conservation Sri Rahayu & Yulian Fakhurrozi ............................................................................ 22

Exploration of Indonesian Nutmeg Seed and Its Active Compound for Management of

Arthritis Inflammation Yanti, Yustine, Marlin Elza, and Hans Christian Leman ........................ 23

Health Percentage Of Coral Reef In Tunda Island, Banten Province Mujiyanto and Joni

Haryadi ............................................................................................................................................ 24

Development of Bamboo Propagation for Source Material of Bamboo-based Activated

Carbon Riksfardini A. Ermawar, Widya Fatriasari, Rikno Harmoko, N. Sri Hartati, A. Dany

Sunandar, Ratih Damayanti, Saptadi Darmawan ............................................................................ 25

Wood Quality of Kelempayan and Benuang from East Kalimantan, Indonesia: Fiber Quality

and Juvenile-Mature Boundary Sari Delviana Marbun, Imam Wahyudi, Jajang Suryana,

Deded Sarip Nawawi ........................................................................................................................ 26

Page 10: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

BSD City – Tangerang, Indonesia – 24th October, 2019

viii

The Effects of FeCl3 Concentration on Hydrothermal Pretreatment of Oil Palm Fronds to

Enhance Reducing Sugar Production Nurwahdah, Rodiansono, A Kamari, R Abdullah,

Sunardi ............................................................................................................................................ 27

The potential for using corn husk as agricultural waste for particleboard raw material

Kurnia Wiji Prasetiyo, Lekianus Zalukhu, Lilik Astari and Dede Hermawan ................................. 28

The Effect Application of Organic Fertilizer and Dolomit Toward Cassava Production in

Banten Province-Indonesia Viktor Siagian, Ulima Amanda, Eko Kardiyanto ............................. 29

Bioconversion of artemisinin by endophytic fungi associated Artemisia sp. grown in Indonesia

and Japan Shoji Maehara, Ahmad Fathon, Muhammad Ilyas, and Andria Agusta ................... 30

Isolation of bioactive compounds from Hyptis suaveolens (L.) Poit. and its antioxidant activity

Swe Swe Aye, Khin Thant Sin .......................................................................................................... 31

Cosmeceutical Potency of Functional Ripe Cider Buni Sthefanie Marchellia, Raymond

Rubianto Tjandrawinata, and Yanti ................................................................................................. 32

POSTER SESSION .......................................................................................................................... 33

Diversity of Epipelic Diatom in Telaga Pengilon Dieng, Indonesia Kenanga Sari and Tri

Retnaningsih Soeprobowati ............................................................................................................. 34

Lipase Production by Paecilomyces sp. BK1 on Olive Oil I Made Sudiana, Atit Kanti, Idris ..... 35

Forest Management Prospects Through Development Non-Timber Forest Products

Gusmailina & Suwardi Sumadiwangsa ........................................................................................... 36

Chemical Characterization of Wood Vinegar Obtained from Acacia Bark Arief Heru

Prianto, Budiawan, Yoki Yulizar and Partomuan Simanjunta ......................................................... 37

Optimization of Pulp Concentration for Bioethanol Production from Elephant Grass

(Pennisetum purpureum) with Addition Tween 20 and Using Two Commercial Yeasts Ina

Winarni and Dwi Wahyuni ............................................................................................................... 38

Two Chemotypes of Sindora leiocarpa Essential Oil and Evaluation of Its Antimicrobial and

Antioxidant Activity Dewi Wulansari, Kartika Dyah Palupi, Praptiwi, Hiroshi Kamitakahara,

Toshiyuki Takano, and Andria Agusta ............................................................................................. 39

SNAP (Single Nucleotide Amplified Polymorphism) Marker Development Based on Eg-Le

(Length) Gene for Early Identification of Low Height Increment in Oil Palm Annisa S.

Salsabila Diny Dinarti Roberdi and Sudarsono ............................................................................... 40

Diversity Of Stands Type At Buffer Zone Karst Citatah Muhamad Yusup Hidayat ................... 41

Phytochemical Content and Antioxidant Activities of Medicinal Plants from North Sumatra

Gunawan Pasaribu and Totok K. Waluyo ........................................................................................ 42

The Comparison of different diatom digestive method using HCl, H2O2 and HCl, KMNO4 in

Telaga Pengilon Dieng Christopher Hardian Widyanta and Tri Retnaningsih Soeprobowati ....... 43

PE Goat Performance in the Breed Stock Area, Banjarmangu District, Banjarnegara Regency,

Central Java Province Tatan Kostaman, Angga Ardhati Rani Hapsari, Singgih Setiawan,

Bess Tiesnamurti, and Anneke Anggraeni ....................................................................................... 44

Screening Phytochemical Compound of Alstonia scholaris R.Br in Different Sites in Indonesia

Zuraida and Sulistiyani.................................................................................................................... 45

Page 11: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

BSD City – Tangerang, Indonesia – 24th October, 2019

ix

Current Status and Species Diversity of Seagrass on Panjang Island, Banten Yayuk Sugianti

and Mujiyanto .................................................................................................................................. 46

Effect of Coconut Water and Extract of Banana Hump on The Growth of Binahong

(Anredera cordifolia (Ten) Steenis) Accessions from the Lowland Yaya Hasanah, Lisa

Mawarni, Chairani Hanum, Nurhaida............................................................................................. 47

Evaluation of The Potency of Endophytic Fungi Associated with Artemisia annua and

Artemisia vulgaris as Antibacterial and Antioxidant Evana, Praptiwi, Ahmad Fathoni,

Muhammad Ilyas, Shoji Maehara and Andria Agusta ..................................................................... 48

Distribution of Long-Tailed Monkeys (Macaca fascicularis) in Kelimutu National Park

Ridwan Fauzi, Tonny Wuryanto, Endarto, Fendra Suarmadi, and Albertus Tomonob .................... 49

Local Rice Conservation Through the Local Wisdom of Indigenous Culture at Kasepuhan

Cisungsang Banten Province Pepi Nur Susilawati, Zuraida Yursak and Ulima D Amanda ......... 50

In vitro response of patchouli in a medium by addition of Methylobacterium spp filtrate

Mia Kosmiatin and Seli Salma ......................................................................................................... 51

Legislation on genetically modified animals and its application possibility in Indonesia

M. Ikhsan Shiddieqy, Bess Tiesnamurti and Sri Muharsini ............................................................. 52

Optimization of Xylose Production from Sugarcane Trash by Microwave-Maleic Acid

Hydrolysis Euis Hermiati, Maulida Oktaviani, Riksfardini Annisa Ermawar, Raden Permana

Budi Laksana, Lutfi Nia Kholida, Ahmad Thontowi, and Siti Mardiana ......................................... 53

Estimation of Biodiesel Production from Used Cooking Oil of University Canteen to Support

Sustainable Electricity in Universitas Pertamina Ari Rahman, I Wayan Koko Suryawan,

Ariyanti Sarwono, Nurulbaiti Listyendah Zahra, Ziyadatulkhair M. Faruqi ................................... 54

The Effect of Sucrose Concentrations and Shoot Inoculum Density on The Growth of In Vitro

Shoot of Teak (Tectona Grandis L.) ** Witjaksono, Indira Riastiwi, Katarina Utami

Nugraheni dan Gut Windarsih ......................................................................................................... 55

Screening of Antibacterial and Antioxidant Activities of Stingless Bee (Tetragonula laeviceps

Smith) Extracts Collected from Purwodadi Botanical Garden – East Java, Indonesia Oscar

Efendy, Ahmad Fathoni, Praptiwi, Dewi Wulansari, Wibowo Mangunwardoyo, and Andria

Agusta .............................................................................................................................................. 56

Diversity of The Ecletus Parrot (Ecletus roratus) Birds Inferred From Mitochondraial Gene

Sequences Dwi Astuti .................................................................................................................... 57

Distribution of Long-Tailed Monkeys (Macaca fascicularis) in Kelimutu National Park

Ridwan Fauzi, Tonny Wuryanto, Endarto, Fendra Suarmadi, and Albertus Tomonob .................... 58

Classification of Agarwood Product from Riau – Indonesia using Near Infrared Spectroscopy

Danang Sudarwoko Adi, Dwi Ajias Pramasari, Bernadeta Ayu W, Adik Bahanawan, Yusup Amin,

Sukma Surya K, Wahyu Dwianto, Hwang Sung-Wook and Junji Sugiyama .................................... 59

Identification and Fiber Morphology Measurements of Enggano Island Woods Species

Wahyu Dwianto, Danang S. Adi, Teguh Darmawan, Adik Bahanawan, Eka Lestari, Yusup

Amin, Dwi A. Pramasari, Fitria, Ratih Damayanti, Sri Rulliaty, Junji Sugiyama ........................... 60

Optimum Tempertature for Germanation and Seedling Characterization of The Three Millet

Accessions N Setyowati, P Lestari and A H Wawo ......................................................................... 61

Page 12: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

BSD City – Tangerang, Indonesia – 24th October, 2019

x

Pcr Column Contamination Gave False Positive Result for Cherax Quadricarinatus

Densovirus (Cqdv) In Sf 9 Cell Cultures Dewi Syahidah, Jennifer Elliman, and Leigh Owens .. 62

Economic Valuation of Forest Fruits Utilization in Batak Toba Community Alfonsus H

Harianja, Anisse M. Sinaga, Ferry A Hawari and Ridwan Fauzi .................................................... 63

Probiotic Powder Production for Cattle by Using Response Surface Methodology

Rohmatussolihat, Roni Ridwan, Nurul Fitri Sari, Rusli Fidriyanto, Wulansih Dwi Astuti,

Yantyati Widyastuti........................................................................................................................... 64

The Effects of Clove Oil On Coral, Trachyphyllia Geoffroy, Using Biomarkers; Color Change

of Coral, Density And Mitotic Index of Zooxanthellae Sri Wahyuni Rahim; Khusnul Yaqin;

Hadiratul Kudsiah ........................................................................................................................... 65

Identification and Test of Fungi On Kaliandra (Calliandra calothyrsus) Seed Which Is

Potential As Antagonist Agent Tati Suharti, Wildani Hanifah, Sri Listiyowati, YMM Anita

Nugraheni, Danu ............................................................................................................................. 66

Potency of Indonesian Nutmeg Oil for Combating Halitosis Yanti, Ika Kristiyaningjati, and

Bibiana Widiyati Lay........................................................................................................................ 67

The Prospect of Microorganism in the Silver Nanopaticle Biosynthesis to Enhance Antibiotic

Drug Activity as an Alternative Solution to Combat Resistances Mega Ferdina Warsito .......... 68

Review: Fungal Glycoside Hydrolases of White-rot Fungi for Cellulosic Biofuels Production

Sunardi, Wiwin Tyas Istikowati, Futhoshi Ishiguri, Shinso Yokota ................................................. 69

Comparison of Dissolved Organic Carbon (DOC) and Physicochemical Variables of Peat

Water Among Three Tropical Peat Swamp Forests Siti Sundari ................................................ 70

Agroforestry Practices In The Use of Dry Land In Gunungkidul District, Yogyakarta,

Indonesia Sudomo A. dan Gerhard E. S...................................................................................... 71

Irradiated Foxtail Millet (Setaria italica (L.) P.Beauv) : Agronomic And Physiological

Performance Under Low Light Intensity Titi Juhaeti ................................................................. 72

Morfological Characterization of Local Durian from Banten Province Zuraida Yursak,

Ulima D Amanda, Dewi Widiyastuti, Pepi Nur Susilawati ............................................................... 73

The Diversity Of Garlic Bulbs And Cloves Quantitative Character In Local Garlic Collection

Of East Java AIAT Ratih Sandrakirana, Maureen Chrisye Hadiatry, Baswarsiati ...................... 74

Detection of Adulteration Activities in Edible Bird’s Nest Using NMR Metabolomics Yong

Chin Hong, Syahidah Akmal Muhammad, Fatimatuzzahra Abdul Aziz, Ng Jing Sheng, Fatin

Ilyani Nasir ...................................................................................................................................... 75

Effect of Amphipilic Lignin Derivatives (A-LD) Surfactant Addition on the Fermentation

Process of Sorghum Bagasse Kraft Pulp for Bioethanol Production W Fatriasari, A Karimah,

F Falah, S H Anita .......................................................................................................................... 76

Traditional Beekeeping of Closed Nested Native Honeybees (Apis spp.) From Four Islands of

Indonesia Sih Kahono, Erniwati, Djunijanti Peggie, & Eko Sulistyadi ........................................ 77

Bioplastic from Modified Cassava Starch, Poly Vinyl Alcohol and Citric Acid Firda Aulya

Syamani, Wida Banar Kusumaningrum, Fazhar Akbar and Bernadeta Ayu Widyaningrum ........... 78

Mechanical Properties Investigation of Green Composite from Ramie (Boehmeria nivea (L.)

Gaud) and Epoxy Ismadi, Safira Vitasasti, Sukma Surya Kusumah ............................................ 79

Page 13: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

BSD City – Tangerang, Indonesia – 24th October, 2019

xi

Bioconversion of artemisinin by endophytic fungi associated Artemisia sp. grown in Indonesia

and Japan Shoji Maehara, Ahmad Fathoni, Muhammad Ilyas and Andria Agusta ..................... 80

Larvicidal Activity of Cerbera odollam against Culex quinquefasciatus (Diptera: Culicidae)

Didi Tarmadi, Sulaeman Yusuf, S. Khoirul Himmi, Maya Ismayati, Ikhsan Guswenrivo, Titik

Kartika, Dita Meisyara ..................................................................................................................... 81

Conservation of Kelulut (stingless bees) in East and North Borneo, Indonesia Syafrizal,

Irawan Wijaya Kusuma, Oshlifin Rucmana Saud, Restina Wiandany, and Harmonis .................... 82

Allelopathy of Invasive Species Air Potato (Dioscorea bulbifera L.) and Its Effect on

Germination of Meranti (Shorea selanica (Lam.) Blume Ade Ayu Oksari, Irvan Fadli Wanda,

Gladys Ayu Paramita Kusumah Wardhani ....................................................................................... 83

Morphological Characteristic of Apple Velvet (Dispyros blancoi) at Germplasms Garden of

LIPI Cibinong Collection Puspita Deswina, Novia Delliasari Aliyya Az Zahra, Etty Hesthiati .... 84

Bee Pollen from Stingless Bee (Heterotrigona itama) as anti Acne* Enos Tangke Arung,

Irawan Wijaya Kusuma, Ayu Mitha Sari, Yadi, Sukemi, Nataniel Tandirogang ............................... 85

"How Fisheries Technology Affect Production?" (Case Study: North Gorontalo Regency)

Tikkyrino Kuriawan and Hakim Miftahul Huda .............................................................................. 86

The Effect of Sucrose Concentrations and Shoot Inoculum Density on The Growth of In Vitro

Shoot of Teak (Tectona Grandis l.) ** Witjaksono, Indira Riastiwi, Katarina Utami

Nugraheni and Gut Windarsih ......................................................................................................... 87

Compression Strength of 9 Years-old Platinum Teak Wood Mohamad Gopar, Dimas

Triwibowo, Fazhar Akbar, Wahyu Dwianto, Akihisa Kitamori and Takuro Mori ............................. 88

Anti-tubercular Activity of Natural Product-derived Compounds (+)-1,1’ Bislunatin and

(+)-2,2’-Epicytoskyrin A. Listiana Oktavia, Vagolu Siva Krishna, Estharla Madhu Rekha,

Ahmad Fathoni, Nikhila Meda, Andria Agusta, Dharmarajan Sriram ............................................ 89

Growth and Development of Nine Accessions of Mutant Grass Plants (Pennisetum purpureum

cv. Taiwan) in the Field of Induction Mutation of Gamma Ray Husni, A, Maftuha, A.A.

Fatmawaty, N. Hermita, and M. Kosmiatin ...................................................................................... 90

Rheological and Thermal Behavior of Polypropylene–Kenaf Pulp Composite at Different

Fiber and Compatibilizing Agent Loading Nanang Masruchin, Wida Banar Kusumaningrum,

Sudarmanto, Sasa Sofyan Munawar, Ismadi, Putri Amanda, Resti Marlina, Lisman

Suryanegara, Subyakto, Bambang Subiyanto1 and Arif Nuryawan ................................................. 91

Concoctions Developed from Correlations of Meliponicultured Honey with Ethnoentomology

in Langkawi, Malaysia Abu Hassan Jalil, Mohd Razif Mamat, Azira Muhamad and Nor Azfa

Johari ............................................................................................................................................... 92

Carbon Dioxide Absorption and Physiological Characteristics of Selected Tropical Lowland Tree

Species for Revegetation La Ode Muhammad Muchdar Davis, Nuril Hidayati……… ……… … 93

Important Date ............................................................................................................................... 94

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KEYNOTE SPEAKER

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Toward 2025 Sustainable use of Biodiversity

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Curriculum Vitae: Dr. Ir. Arifin Rudianto, M. Sc

Deputy of Maritime Affairs and Natural Resources Office Tel : (021) 391 6340

Ministry of National Development Planning Office Fax : (021) 314 4131

Jakarta Indonesia Email : [email protected]

Japan 611-0011

EDUCATION

Doctor of Maritime Policy, University of Wollongong Australia.

Master of Marine Science and Coastal Management, University of Newcastle Upon Tyne,

UK.

Bachelor in Agriculture, IPB University.

WORK EXPERIENCES

2003 - 2005 Director of Sectoral and District Affairs

2005 - 2009 Director of Regional

2009 - 2014 Director of Regional Development

2014 - 2015 Assistant of Minister for Spatial Planning and Maritime

2015 Assistant of Minister for Regional and Distribution

2015 - 2017 Deputy of Regional Development

2017 - now Deputy of Maritime Affairs and Natural Resources

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Curriculum vitae : Noriaki Sakaguchi

Senior Advisor, Global Environment Department Office Tel : +81-3-5226-3394

Japan International Cooperation Agency Office Fax : +81-3-5226-6343

Nibancho Center Building, 5-25 Niban-cho,Chiyoda-ku, Email:[email protected]

Tokyo, 102-8012, Japan

FIELD OF RESEARCH

Animal ecology, Biodiversity conservation, Ecosystem management, International policies on

biodiversity

EDUCATION

1989 – 1994 Doctor of Science, Faculty of Science, Kyushu University

Received Ph.D. in Science, March 1994

Title: Social system and ecology of the Iriomote cat Felis iriomotensis

1986 – 1989 Master of Science, Faculty of Science, University of the Ryukyus

Received Master of Science, September 1989

Title: Ecological study of the Iriomote cat Felis iriomotensis

1981 – 1985 Bachelor in Science, Department of Biology, Faculty of Science, University

of the Ryukyus

PUBLICATION 1. Sakaguchi N., 1991, Habitat use of the Iriomote cat and changes in its response to prey

availability, In: Wildlife Conservation (ed. Maruyama, N., Bobek, B., Ono, Y.,

Regelin, W., and Ratcliffe, P.) pp. 137 – 140, Japan Wildlife Research Center, Tokyo.

2. Sakaguchi N., 1994, Ecological aspects and social system of the Iriomote cat Felis

iriomotensis (Carnivora; Felidae), Ph. D. Thesis, 67pp. Kyushu University.

Sakaguchi N. and Ono, Y., 1994, Seasonal change in food habits of the Iriomote cat,

Felis iriomotensis, Ecological Research 9 (2): 167 – 174.

3. Sakaguchi N., Yoneda, M., Sugardjito, J., Effendi, R., Ladjar, N. L. and Suryaningrum,

R., 1997, Preliminary survey of carnivores in Cikaniki area of Gunung Halimun

National Park, Research and Conservation of Biodiversity in Indonesia, Vol. II:

63-80.

4. Sakaguchi N., Yoneda, M., Ladjar, N. L., Sinaga, M. H., 1999, Research and

conservation of the leopard Panthera pardus in Gunung Halimun National Park, West

Java, Indonesia, P: 79 – 83, In Laporan Penyelengaram Ekspose & Lokakarya

Potensi Taman Nasional Gunung Halimun Dan Pemanfaatanya Secara

Berkelanlutan, Bandung, 26-27 Maret, 1999.

5. Syahrial A. H., S. Wijamukti, Koestoto, Sinaga, M. H., Sakaguchi, N., and Okayama,

T., 2001, Leopard (Panthera pardus) monitoring around Cikaniki area in Gunung

Halimun National Park, P.: 39 – 40, In: Simposium dan Seminar Pengelalaan

KeanekaragamanHayati Taman

Izawa, M., Sakaguchi, N. and Doi, T., 2001, The recent conservation program for the

Iriomote cat, Felis iriomotensis, Tropics 10: 79 – 86.

6.

7.

Sakaguchi N., (ed.), 2003, Research on Endangered Species in Gunung Halimun

National Park, Research and Conservation of Biodiversity in Indonesia, Vol. XI,

70pp. Bogor.

Sakaguchi N., Kuswandono, Ekawati, D., Syahrial A. H., and Mulyati, S. (ed.), 2003,

The action plan for the conservation of endangered species in Gunung Halimun

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8.

National Park and its surrounding area, 36pp. Kabandungan.

Syahrial A. H. and N. Sakaguchi, 2003, Monitoring Research on the Javan Leopard

Panthera pardus melas in a Tropical Forest, Gunung Halimun National Park, West

Java, Research and Conservation of Biodiversity in Indonesia, Volume XI: 2-28.

9. Sakaguchi N., Coastal Marine Ecosystem and Blue Carbon, an International

Cooperation for their Conservation in Coral Triangle Area, International Cooperation

of Agriculture and Forestry, Vol.41(2): 2-14, In Japanese

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IPBES Assessments on Bioodiversity and Ecosystem serveces and Toword

Post 2020 Framework and Achievement of SDGs

Noriaki Sakaguchi

Fosret and Nature Conservation Group, Global Environment Department, Japan International

Cooperation Agency,

Nibancho Center Building, 5-25 Niban-cho, Chiyoda-ku, Tokyo 102-8012, Japan

*Corresponding author: [email protected]

Abstract. IPBES published its regional and global assessment reports on biodiversity and

ecosystem services in 2018 and 2019, in respectively. Biodiversity and ecosystem

services, providing foods, regulating the climate and reducing natural disaster risks, are

essential for human existence and good quality of life. However, they are deteriorating

worldwide. 75% of the land surface is significantly altered. About 1 million species

among existing fauna and flora face extinction within decades. In Asia and the Pacific,

the rich biodiversity and valuable ecosystem services support 4.5 billion people (64.3%

of the world population), while they are declining in most of the region due to rapid

economic growth. Forest cover in South-East Asia was largely reduced by 12.9% from

1990 to 2015. Direct drivers of the changes are land/sea use change, over exploitation,

climate change, pollution and invasive alien species. Indirect drivers include production

and consumption, human population, trade, technological innovations and governance.

Regarding the Aichi Targets, the progress in extent of protected areas (Target 11) is

significant, but those in most of other targets are insufficient to meet. It‟s also necessary

to mainstream biodiversity in key economic sectors of agriculture, forestry and fisheries,

tourism, energy and mining, infrastructure, manufacturing and processing. Since

compounding effects of direct and indirect drivers exacerbate negative impacts on

biodiversity and ecosystems, the post 2020 biodiversity targets and SDGs can only be

achieved through transformative changes across economic, social, political and

technological factors. Specifically, integrated approaches through cross-sectoral

cooperation is necessary to enable transformative change.

Keywords : IPBES, biodiverswity, ecosystem services, Aichi Targets, SDGs

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INVITED SPEAKER

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Curriculum vitae : Dr. Lin Chun Yi

Research Instituter for Sustainable Humanosphere Office Tel: +81-774-38-3604

Kyoto University

Gokashou, Uji Email: [email protected]

Japan 611-0011

FIELD OF RESEARCH

My previous research interest was on citrus systemic diseases (citrus huanglongbing, citrus

tristeza, citrus tatter leaf and citrus exocortis) and on understanding the basic characterization

and disease ecology of viroids and how two viroids interact with each other in citrus host. My

recent research topic focuses on the impact of virus spillover from invasive species to native

pollinator community. in the project, I investigate the prevalence and distribution of honeybee

viruses in two global invasive ant species. The interaction between honeybee, virus and

invasive ant can be a standard example for understanding the pathogen transmission route

from native to invasive species.

EDUCATION

2010 – 2015 Ph.D., Department of Plant Pathology and Microbiology, National Taiwan

University

2004 – 2008 B.S., Department of Plant Pathology and Microbiology, National Taiwan

University

PUBLICATION 1. Lin, C. Y., Chen, Y. H., Wu, M. L., Su, H. J., and Hung, T. H. (2018). Pathological

categorization of the stem-pitting and mild strains Citrus tristeza virus in Taiwan and

their genomic analysis. J Phytopathol 166, 459-469.

2. Lin, C. Y., Chang, L., Lin, Y. H., Cheng, H. J., Wu, M. L., and Hung, T. H. (2018).

Biological and molecular characterization of citrus tatter leaf virus in Taiwan. Plant

Pathol 67, 995-1008.

3. Lin, C.Y., Tsai, C. H., Tien, H. J., Wu, M. L., Su, H. J., and Hung, T. H. (2017).

Quantification and ecological study of Candidatus Liberibacter asiaticus in citrus

hosts, rootstocks and the Asian citrus psyllid. Plant Pathol 66, 1555-1568.

4. Lin, C. Y., Wu, M. L., Shen, T. L., Yeh, H. H., and Hung, T. H. (2015). Multiplex

detection, distribution, and genetic diversity of Hop stunt viroid and Citrus exocortis

viroid infecting citrus in Taiwan. Virol J 12, 11.

5. Lin, C. Y., Wu, M. L., Shen, T. L., Yeh, H. H., and Hung, T. H. (2015). Multiplex

detection of Citrus viroids: advances and future application. Br J Virol 2(1), 12-14.

6. Lin, C. Y., Wu, M. L., Shen, T. L., and Hung, T. H. (2015). A mutual titer-enhancing

relationship and similar localization patterns between Citrus exocortis viroid and Hop

stunt viroid co-infecting two citrus cultivars. Virol J 12, 142.

7. Lin, C. Y., Wu, M. L., and Hung, T. H. (2015). Identification of two commercial

tomato cultivars as new indicator plants for detecting Citrus exocortis viroid infection.

Plant Pathol Bull 24(1), 01-11.

8. Wu, M. L., Shen, T. L., Chang, T. T., Chen, C. H., Lin, C. Y., Lee, C. Y., and Hung, T.

H. (2015). Establishment of a rapid PCR detection method for „„Antrodia salmonea‟‟

and „‟A. cinnamomea‟‟. Taiwan J For Sci 30(1), 45-53.

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Emerging threats of invasive ants to native pollinator community: insights

into virus spillover

Chun-Yi Lin and Chin-Cheng Yang*

Research Institute for Sustainable Humanosphere, Kyoto University

Gokasho, Uji, Kyoto 611-0011, Japan

*Corresponding author: [email protected]

Abstract. Emerging virus diseases consistently threaten pollinator species worldwide.

Recent studies have shown that ants often are infected by honeybee viral pathogens likely

during their interactions with honeybee, suggesting that cross-species transmission of

honeybee viruses may have occurred commonly than previously thought. However, these

studies are either at regional level or focused on only one or two honeybee viruses. We

describe six honeybee viruses in two most widespread and damaging invasive ants (Yellow

crazy ant (Anoplolepis gracilipes) and Longhorn crazy ant (Paratrechina longicornis) in

Asian regions. Depended on the ability of fast reproduction and well-adaptation to new

environment, virus spillover could occur from invasive ants, vector for virus reservoirs, to

local honeybee populations. Foreign lethal viral species may be introduced to pollinator

community via cross-species interaction and have severe impacts to native ecosystem. The

Large-scale survey of honeybee viruses in invasive ants provide insight to break point in

pathogen prevention and also offer new information for possible transmission routes

between invasive and pollinator species.

Keywords : Anoplolepsis gracilipes, Paratrechina longicornis, virus spillover, honeybee

virus

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Curriculum vitae : Prof. Subyakto

Research Center for Biomaterials, Indonesian- Office Tel : +62-21-87914511

Institute of Sciences Office Fax: +62-21-87914510

Jl. Raya Bogor Km 46, Cibinong, Bogor 16911 Email:[email protected]

Indonesia

FIELD OF RESEARCH

Biocomposites

EDUCATION

1999 – 2002 Doctor of Agriculture, Kyoto University, Japan

.

1987 – 1990 Master of Forest Science, The University of Melbourne, Australia

.

1978 – 1982 Bachelor in Forestry, Bogor Agriculture University, Indonesia

PUBLICATION 1. Budiman, I., Hermawan, D., Febrianto, F., Subyakto, Pari, G. 2019. Char properties

and pollutant adsorption capability of oil palm shell using hydrothermal process.

Biomass Conversion and Biorefinery. doi: 10.1007/s13399-019-00394-5

2. Endrawati, Y.C., Solihin, D.D., Suryani, A. Subyakto. 2019. Improving Poly (Lactic

acid) Properties by Using Fibre Reinforcement of Wild Silkworm Attacus atlas.

Pakistan J. Sci. Ind. Res. Ser. A: Phys. Sci. 62A (1): 38-47

3. Budiman, I., Hermawan, D., Febrianto, F., Subyakto, Pari, G. 2019. Optimal activation

of activated char from oil palm fruit shell using response surface methodology. Journal

of Tropical Wood Science and Technology. 17(1): 8-21

4. Prasetiyo, K.W., Octaviana, L., Astari, L., Syamani, F.A., Subyakto, Achmadi, S.S.

2018. Physical-Mechanical Properties and Bonding Mechanism of Corn Stalks

Particleboard with Citric Acid Adhesive. Journal of Tropical Wood Science and

Technology. 16(2):132-141

5. Suryanegara, L., Yuliansih, P., Syamani, FA., Laksana, RPB., Subyakto, Abidin, Z.,

Masruchin, N., Indrayani, M. 2018. Effect of Phenylphosphonate Acid – Zinc, talk and

Triacetine on crystalline speed of polylactid acid. J. Sains Materi Indonesia.

19(3):93-97

6. Yuni Cahya Endrawati, Dedy Duryadi Solihin, Ani Suryani, Subyakto. 2017.

Optimization of Fibroin Yield of Bombyx mori L. dan Attacus atlas L. using Response

Surface Methodology. Agritech Jurnal Teknologi Pertanian. 37 (2): 205-214

7. Wida Banar Kusumaningrum, Rochmadi, Subyakto. 2017. Preparation of Acetylated

Cellulose from Pulp of Betung Bamboo (Dendrocalamus asper) and its effects on

mechanical properties of polypropylene composites. Reaktor 17 (1): 25-35

8. Dungani, R., Karina, M., Subyakto, Sulaeman, A., Hermawan, D., Hadiyane, A. 2016.

Agricultural Waste Fibers Towards Sustainability and Advanced Utilization: A Review.

Asian Journal of Plant Sciences 15(1-2):42-55

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9. Pramono, A.E., Rebet, I., Zulfia, A., Subyakto. 2015. Tensile and shear punch

properties of bamboo fibers reinforced polymer composites. International Journal of

Composite Materials 5(1):9-17

10. Syamani, F.A., Subyakto, Sukardi, Suryani, A. 2015. Changes in oil palm frond fiber

morphology, cellulose crystallinity and chemical functional groups during cellulose

extraction phases. Chemistry and Materials Research 7(3):105-113

11. Ismadi, Yuwono, A.H., Astutiningsih, S., Subyakto. 2015. Effect of Carbon Fiber

Loading in Mechanical Properties and Electrical Conductivity of Polyvinyl Alcohol

Based Composites. Macromolecular Symposia 353(1):102–107

12. Nugroho, A., Satyarno, I., Subyakto. 2015. Bacteria as Self-Healing Agent in Mortar

Cracks. J. Eng. Technol. Sci. 47 (3): 279-295

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Biocomposites from Natural Fibers

Subyakto*, Nanang Masruchin and Sukma Surya Kusumah

Research Center for Biomaterials, Indonesian Institute of Sciences,

Jl. Raya Bogor Km 46, Cibinong-Bogor 16911, Indonesia

*Corresponding author: [email protected]

Abstract. Indonesia has big potential of natural fibers from many sources of raw

materials such as original natural fibers, wastes of agriculture, estates, and forestry

activities. Waste from oil palm industry is the biggest source of fiber materials, for

example empty fruit bunches, trunks, and fronds. The natural fibers and wastes are not

optimally utilized; therefore they are potential to be used as raw materials of composites

industry to make added values. In comparison with petroleum based synthetic fibers,

natural fibers have some advantages; such as lightweight, low cost, sustainable,

recyclable, and biodegradable. In addition they have good thermal and acoustic properties,

machine friendly (less abrasive), and easier to process. Many composites materials from

natural fibers had been developed in Indonesia mainly in the laboratory scale.

Particleboard, medium density fiberboard, laminated board, fiber-cement board,

fiber-plastic composite, and molding product have been developed. Composites from

bamboo, corn stalk, kenaf, pineapple, ramie, sugarcane bagasse, sorghum bagasse, empty

fruit bunches, trunks, and fronds have been subjected to many research in Indonesia.

Adhesive is very important factor in manufacturing of natural fiber composites;

preferably it is formaldehyde free, low cost, and has excellent performances. Citric acid

or combination of citric acid and sucrose had been developed as environmentally friendly

adhesive for particleboards. Natural fiber composites have many applications such as

building components, furniture, automotive parts, medical applications, packaging, and

sport goods. Utilization of natural fibers will be more and more significant in the future.

The future trends of utilizations of natural fibers among them are bio-composites,

nano-fiber-composites, and advanced materials such as supercapacitors.

Keywords : biocomposites; natural fibers; utilization, Indonesia

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Curriculum vitae : Dr. Elpidio V. Peria

ASEAN CENTRE FOR BIODIVERSITY Office Tel : +6349-536-2865

Domingo M. Lantican Avenue, College, Office Fax: +6349-584-4210

Los Baños, Laguna 4031 Philippines Email: [email protected]

FIELD OF WORK

Biodiversity policy formulation, development and advocacy– international, national, local –

SEAsia and Philippines

EDUCATION

1991-1995 Bachelor of Laws, University of the Philippines, Diliman, Quezon City

1983-1987 Bachelor of Science in Fisheries, major in Fisheries Business Management,

University of the Philippines, Diliman, Quezon City

EXPERIENCE

Years

1997-present

Over 2O years of litigation experience, including policy advocacy and

formulation; treaty negotiations in Nagoya Protocol and art. 10 of Paris

Agreement on Technology Development and Transfer

Regular blog writer since 2012

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Balancing economic development and biodiversity protection – Biodiversity

value chains & certification of products

Peria, Elpidio1 ; Schlegel, Bianca2 ; Dr. Simorangkir, Dicky3

1 ASEAN Centre for Biodiversity (ACB); Los Baños, Laguna, Philippines *

2 GFA Consulting Group, Hamburg, Germany

3 Deutsche Gesellschaft für International Zusammenarbeit (GIZ) GmbH, Eschborn, Germany

*[email protected]

Abstract. ASEAN´s wealth of biological resources poses immense opportunities for

economic development. The ASEAN Member States want to use this economic potential

by further developing sustainable trade relations for bio-products nationally, regionally and

possibly globally. However, the exact market potential of Biodiversity-based Products

(BBP) is often still unknown. The “Biodiversity-based Products (BBP) as an Economic

Source for the improvement of Liveli-hoods and Biodiversity Protection” Project, funded

by the German government via the Deutsche Gesellschaft für International

Zusammenarbeit (GIZ) GmbH, is implemented in cooperation with the ASEAN Centre for

Biodiversity (ACB) by GFA Consulting Group, to pilot biodiversity-based value chains in

Cambodia, Laos and Vietnam, promoting conservation through sustainable use. Villagers

around protected areas, local park management, cooperatives and private sector business

partners cooperate for the development of marketable products where they did not yet exist

or for upgrading of existing products. Biodiversity value chains starts with sustainable

sourcing of raw material, including management plans for the input resources which also

comprises in-situ and ex-situ planting to enhance available raw material. Value is added

through enhanced processing steps and especially certification (e.g. ODOP certification for

bamboo) which improves market access for the villagers and their products.

Keywords : biodiversity value chains; biodiversity-based products; biological resources;

certification

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Curriculum vitae : Ms Margarita Naming

Sarawak Biodiversity Centre Tel: 00 6 082 610610

KM20, Jalan Borneo Heights Fax: 00 6 082 611535

Semengoh Email: [email protected]

Locked Bag No. 3032

93990 Kuching Sarawak

Malaysia

FIELD OF RESEARCH

leading the Traditional Knowledge Documentation programme at the Sarawak

Biodiversity Centre (SBC)

spearheaded the development and implementation of Access (Prior Informed Consent)

and Benefit Sharing with various ethnic communities in Sarawak based on the State‟s

biodiversity law on Access and Benefit Sharing (ABS)

leads a team in developing and implementing the Sarawak Biodiversity Centre Ordinance

1997 and Sarawak Biodiversity Regulations 2016.

EDUCATION

2015 Masters in Environmental Sciences from Universiti Malaysia Sarawak

(UNIMAS)

1997 Degree in Zoology from the National University of Malaysia (UKM)

PUBLICATIONS

“Building a discovery partnership with sarawak biodiversity centre: a gateway to access

natural products from the rainforests”

Authors: Chia Yeo, Tiong; Naming, Margarita; Manurung, Rita

Source: Combinatorial Chemistry & High Throughput Screening, Volume 17, Number 3,

2014, pp. 192-200(9)

“Maximizing Biodiversity Potential Through Traditional Knowledge documentation: A

Study Case”

Authors: Jern Ern Chuah, Rita Manurung, Margarita Naming

Source: Journal of Intellectual Property Law & Practice, Volume 9, Issue 5, May 2014,

Pages 397–402, https://doi.org/10.1093/jiplp/jpu041

Speaker in International Workshops relating to ABS

(i) 2019 Southeast Asia Symposium of Traditional Medicine: Experience Sharing &

Future Cooperation, 13 - 14 July 2019, National Yang-Ming University School of

Medicine, Taiwan,

(ii) ASEAN Conference on Biodiversity 2016, 15-19 February 2016, Bangkok, Thailand

(iii) Bhutan National Seminar on Traditional Knowledge Associated with Biological

Resources, 29 - 30 March 2016, Bhutan

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(iv) Regional Workshop on Access to Genetic Resources and the Fair and Equitable Sharing

of Benefits Arising from their Utilization (ABS): Understanding the Nagoya Protocol,

25-26 October 2011, Manila

(v) Southeast Asia Training-Workshop on Building Institutional and Stakeholders

Capacities on Access to Genetic Resources and the Fair and Equitable Sharing of

Benefits Arising from their Utilization (ABS), 20 to 23 August 2012, The Imperial

Queen‟s Park Hotel, Bangkok, Thailand

Participation in International Meetings on ABS

Ninth Conference of Parties to the Convention on Biological Diversity (COP-9) from 19

to 30 May in Bonn, Germany

7th Ad-Hoc Working Group on Access and Benefit Sharing of Biological & Genetic

Resources (ABS) in Paris from 2 to 8 April 2009

Resumed Meeting on Working Group of Access and Benefit Sharing (16-17 October

2010) and the Tenth Conference of the Parties to the Convention on Biological

Diversity (CBD COP 10) from 18 to 29 October 2010 in Nagoya, Japan. During the

COP 10 meeting, the Nagoya Protocol on ABS was adopted by the Parties.

12th Conference of Parties to the Convention Biological Diversity (COP-12), Pyeong

Chang, Korea.

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Successful Experience and Gaps in Access and Benefit Sharing (ABS)

through Research on Traditional Knowledge in Sarawak, Malaysia

Margarita Naming* and Yeo Tiong Chia

Sarawak Biodiversity Centre KM20, Jalan Borneo Heights, Semengoh, Locked Bag No. 3032,

93990 Kuching Sarawak

Malaysia

*Corresponding author: [email protected]

Abstract. After more than two decades since 1992, the third objective of the Convention

on Biological Diversity (CBD), is recognized with the coming into force of the Nagoya

Protocol on Access to Genetic Resources and the Fair and Equitable Sharing of Benefits

Arising from their Utilization in 2014. This Protocol “only” provides the core obligations

framework for contracting parties in relation to access, benefit-sharing and compliance

which is the “ABC” of the Nagoya Protocol. In Sarawak, the CBD objectives were

applied through the Sarawak Biodiversity Centre Ordinance in 1997. The Ordinance

provides clear and current provisions on traditional knowledge (TK) documentation and

bioprospecting and subsequently provisions on access and benefit sharing (ABS) in line

with the Nagoya Protocol. This paper shares the beginning to an end of a successful case

in the implementation of ABS which include Prior Informed Consent (PIC) and sharing of

benefits; an effort that took fourteen (14) years. The pilot projects on TK and ABS clearly

shows that feasibility as well as the value can be derived. The communities need an

establishment of trust to agree to such a project, and are motivated by continuous

engagement and empowerment. The projects also identify various challenges in

determining royalties and margins, managing third party involvement, multiple

communities with a similar genetic resource, value disruption, supplies of raw materials,

sustainability, scale-up, disbursement of monetary benefits and community participation.

Keywords : Access and Benefit Sharing, Prior Informed Consent, Sarawak Biodiversity

Centre, traditional knowledge documentation, bioprospecting

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Curriculum vitae : Mr. Erlambang Ajidarma

Erlambang Ajidarma is head of product innovation at Mycotech. He received BSc. in

Microbiology (2012) at Institut Teknologi Bandung. He has been work at Mycotech since

2016 developing biomaterial from waste agriculture and work together with ETH Zurich and

Future Cities Laboratory - National University of Singapore in Mycotree development for

Seoul Biennale 2017. His focus research right now in Mycotech is developing mushroom

composite board and leather-like material from agricultural waste.

EXPERIENCE

1. January 2017 to Current : Miko Bahtera Nusantara Bandung (Mycotech), Indonesia

Head of Product Innovation

Development of biomaterial as structure material from agricultural waste using

mushroom as binder (Future Cities Laboratory – Singapore-ETH Zurich)

Mushroom waste as oil adsorbent (Pertamina)

Mushroom waste as biobricket (Pertamina)

Substrate effect to fungal composite materials

Growth optimizing of fungal polypore in producing mycelial sheet

2. April 2013 to December 2016 : Charoen Pokphand Indonesia Jakarta, Indonesia

Research Assistant

Isolation and assessment of lactic acid bacteria from free range chicken as poultry

probiotic

Growth optimizing of photosynthetic bacteria in batch reactor system

Assessment of photosynthetic bacteria as H2S reducing agent in aquaculture pond

EDUCATION

2012 Institut Teknologi Bandung Bandung – Bachelor of Science Microbiology

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Mycelium-Composite as Future Biomaterial

Erlambang Ajidarma

Mycology Researcher

Mycotech

[email protected]

Abstract. Indonesia as an agricultural country has abundant crop and plantation products.

Based on data from Indonesia‟s Ministry of Agriculture, in 2017, Indonesia has

productive plantations of 25 million hectares with production value up to 46 million tons

for palm oil, coconut, rubber, coffee, and cocoa. This huge production value generates

agricultural wastes up to 14 million tons of biomass. Fungal, with its ability to digest

cellulose and lignin, can play an important role in utilizing the agricultural wastes.

Mycelium, root structure of fungal, acts as natural glue that can bind organic materials

together and transform it into natural biocomposite called mycelium-composite. By using

fungal as a binding agent, agricultural wastes like baggase, sawdust, and woodchip can be

converted into mycelium-composite that has flame retardant and good insulation

properties. Furthermore, providing physical treatment such as hot-pressed system to

mycelium-composite can alter the composite into binderless board with strength

equivalent to particle board. Although mycelium-composite is a promising biotechnology

product, its development as a biomaterial is still early considering there are many aspects

that can be explored, such as combination of agricultural wastes, type of fungus used, as

well as types of fungus growing methods.

Keyword : agricultural waste, fungal, natural glue, mycelium composite

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ORAL PRESENTATION

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20

Honey, a persisting NTFP on a deforested peat landscape of eastern

Sumatra

Ahmad Muhammad 1* Abdul Ronny

2 and Yasuyuki Kono

3

1Dept. Biology, Fac. Math. & Natural Sciences, Riau University, Pekanbaru, Indonesia

2Center for Southeast Asian Studies (CSEAS), Kyoto University, Kyoto, Japan

*Corresponding author / email: [email protected]

Abstract. The peat landscapes of eastern Sumatra have undergone severe deforestation,

which has deprived the areas of valuable forest products, such as timber and various

non-timber forest products (NTFPs). The present study focused on the honey of Apis

dorsata, which is one of the most important NTFPs. Despite the loss of natural forests, we

recently observed honey gathering activities on Bukit Batu peat landscape in Bengkalis

Regency, Riau Province. The giant honeybee adaptively responds the loss of large and tall

trees by building solitary nests in smaller trees in non-forest vegetations. We conducted

both explorative and plot-based systematic surveys at three villages to assess the nest

distribution and density. A total of 146 nests were detected in young secondary forests

(38.4%), rubber plantations (37.0%), oil palm plantations (21.9%), and home gardens

(2.7%). The average nest density in such vegetation mosaic systems was 0.23 nests/ha.

During „honey seasons‟ the average number of nests cut in each village was 26.6

nests/month and the average amount of honey collected was 137.6 kg/ month, which

potentially fetched a collective income of about 11 million IDR/month. The findings

suggest that production of forest honey in peatland areas may persist despite the loss of

natural forests.

Keywords : Apis dorsata; Bukit Batu; honey gathering; peatland area; vegetation

mosaic

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A Preliminary Study on The Population and Habitat of Saltwater Crocodile

(Crocodylus porosus) in Timor Island, East Nusa Tenggara

Grace Serepina Saragih*1,2

, Kayat2, Muhammad Hidayatullah

3,2 , Dani Sulistyo Hadi

2

1Research and Development Center for Environmental Quality and Laboratory

Gd. 210, Komplek Puspiptek, Serpong, South Tangerang, Indonesia 2Kupang

Research and Development Institute for Environment and Forestry

Jl. Alfons Nisnoni No.7, Kupang, East Nusa Tenggara, Indonesia 3Research and Development Institute of Technology Non Timber Forest Product

Jl. Dharma Bakti No.7, Lombok Barat, West Nusa Tenggara, Indonesia

*Corresponding author : [email protected]

Abstract. The information on the population size and habitat condition of Saltwater

crocodile in East Nusa Tenggara is unavailable. The present paper discusses preliminary

data on population and habitat characteristic of the Saltwater crocodile in three

conservation areas in Timor island, namely Teluk Kupang Marine Nature Park, Menipo

Nature Park, and Maubesi Mangrove Forest Nature Preserve. Spotlight surveys and

vegetation analysis were carried out in each location. Crocodile encounter rate was

calculated as individuals detected per km river surveyed. The encounter rate in Teluk

Kupang, Menipo and Maubesi was 0.3 crocodiles/Km, 0.4 crocodiles/Km, and 0.6

crocodiles/Km respectively. Hatchlings, juveniles and adult saltwater crocodile were found

in in Menipo and Maubesi but only adults were found in Teluk Kupang. Crocodiles prefer

less saline waters and sheltered areas such as mangrove roots. Mangrove density in

Teluk Kupang, Menipo and Maubesi was 127 trees/ha, 124 trees/ha and 186 trees/ha

respectively. These were the first systematic surveys in the areas studied and additional

work is needed to further characterize the population and habitat of saltwater crocodile in

East Nusa Tenggara.

Keywords : habitat, population, saltwater crocodile, Timor

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High Corolla Colour Variation of Hoya coronaria in Belitung Island:

Potential Use and Conservation

Sri Rahayu1)

* & Yulian Fakhurrozi2)

1) Research Center for Plant Conservation and Botanic Gardens- Bogor Botanic Gardens,

Indonesian Institut of Sciences (LIPI), Jl. Ir. H. Juanda 13 Bogor- 16122 2)

Badan Pengelola Geopark Pulau Belitung – sektretariat Belitung Timur, Kabupaten Belitung

Timur, Kepulauan Bangka Belitung 33512

*contact person, email: [email protected]; [email protected]

Abstract.The genus Hoya (Apocynaceae: Asclepiadadoideae) has become popular as

ornamental plant due to the beauty of its shiny, waxy, star shaped corolla with various

colour. Normally, each species has specific corolla and corona colour. But some species

has variation in corolla colour, for example Hoya coronaria Blume. Normally, H.

coronaria has white with pinkish dotted corolla colour, rarely with red corolla colour. H.

coronaria distributed from South Thailand, Malay Peninsula, Java, Borneo and Sumatra

as well as in Belitung Island. We surveyed the population and corolla colour of H.

coronaria in Belitung Island in June. The RHS colour chart was used to determine the

colour. We found 8 accession with differen corolla and corona colour variation of H

coronaria in Belitung Island, ranging from white, cream, pink, peach, deep pink, yellow,

red, and deep red. This variation was very high and population of Hoya coronaria well

developped in the heath forest habitat of Belitung Island. The further utilization was

suggested as domesticated ornamental plant with proper characterization to support

further breeding program. H coronaria was also a component of heath forest habitat of

Belitung Island, wich is very fragile, thus the ex situ and in situ conservation was

suggested for sustainibility.

Key words: bioresource, flower, germplasm, Heath forest, Hoya coronaria, variavility

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23

Exploration of Indonesian Nutmeg Seed and Its Active Compound for

Management of Arthritis Inflammation

Yanti*, Yustine, Marlin Elza, and Hans Christian Leman

Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta 12930,

Indonesia

*Corresponding author: [email protected]

Abstract. Gout or arthritis inflammation is caused by the accumulation of monosodium

urate in various connective and joint tissues that lead to the formation of uric acid. The

presence of uric acid induces local inflammation and neutrophile production by

over-expression of pro-inflammatory cytokines, including tumor necrosis factor (TNF)-α,

inducible nitric oxide synthase (iNOS), and interleukin (IL)-6. The use of chemical drugs

for gout treatment causes side effects, like liver and kidney failure, and hypersensitivity.

Thus, finding natural theurapeutics from plants has been the focus of investigation. Here,

we investigated whether nutmeg extract (Myristica fragrans) and its active compound,

macelignan, inhibited the expression of pro-inflammatory cytokines in

lipopolysaccharide (LPS)-induced chondrocytes in vitro by conducting MTT,

SDS-PAGE, and ELISA analysis. MTT profile showed that nutmeg extract and

macelignan up to 10 µg/ml only had less toxicity on cell viability and cell morphology.

SDS-PAGE profile demonstrated that chondrocytes treated with LPS secreted TNF-α

protein with molecular weight of 51 kDa. ELISA data showed that treatment of nutmeg

extract and macelignan dose-dependently inhibited TNF-α, iNOS, and IL-6 expression in

LPS-induced chondrocytes, as well as ibuprofen standard. In conclusion, nutmeg extract

and its macelignan may provide the natural anti-gout arthritis potency for management of

arthritis inflammation.

Keywords: arthritis inflammation, chondrocytes, macelignan, nutmeg, anti-gout effect

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24

Health Percentage Of Coral Reef In Tunda Island, Banten Province

Mujiyanto*) and Joni Haryadi

*) Research Institute for Fisheries Enhancement

Cilalawi Street No. 1, Jatiluhur Purwakarta, West Java Indonesia – 41152

*Corresponding author : [email protected]

Abstract. Coral reef ecosystem is once important ecosystem to support people life in

coastal areas. Once of community has been dependencing coastal areas especially coral

reef ecosystem is community in Tunda Island, it was because around 80 % people in

Tunda Islands were dependencing their livelihood in coral reef areas. The degradation of

coral reef habitat was in Tunda Island to give out significant effect for people life in

Tunda Island. The benefits that is coral reef ecosystem potency, being the people has

been dependency from resources. This study aimed to now condition habitats and

analysis of coral reef ecosystem potency. It was conducted in July 2018, July 2019 and

September 2019. The method survey was underwater photography transect method at 2

depths areas were ± 3-4 m and ± 10-11 m. Result analysis of coral reef percentage cover

reef cover were in 8 categories group base from Coral Point Count with Excel extensions

(CPCe) seri 4.1. output. Values of percentage coral reef cover in first depth (± 3-4 m) has

found percent cover of health reef that is grouping in category Coral (C) on each side of

Tunda Island are 75,39 % (east side‟s island), 51,81 % (north side‟s island), 37,90 %

(west side‟s island) and 66,42 % (south side‟s island). The percentage of coral reef on the

east side was 75.39 % include in very good of health category. The result of heath

category in second depth also was founded in east side‟s island with the value was 58,87

% (good category). Different of percentage covers value was suspected more affects from

people activities for did their activities in coastal areas especially in coral reef ecosystem.

Keywords: present cover, habitats, coral reef, tunda island

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Development of Bamboo Propagation for Source Material of Bamboo-based

Activated Carbon

Riksfardini A. Ermawar1*

, Widya Fatriasari1, Rikno Harmoko

2, N. Sri Hartati

2, A. Dany

Sunandar3, Ratih Damayanti

3, Saptadi Darmawan

3

1Research Center for Biomaterials, Indonesian Institute of Sciences,

2Research Center for Biotechnology, Indonesian Institute of Sciences,

1,2Cibinong Science Center, Cibinong-Bogor 16911, Indonesia

3Forest Product Research and Development Center, Ministry of Environment and Forestry

(KLHK), 3Jl. Gunung Batu No. 5 - Bogor 16610, Indonesia

*Corresponding author: [email protected]

Abstract. Bamboo species are widespread in many regions in Indonesia. They are

becoming an important part of Indonesian cultural and social life. They have been

vastly-used and have economic value as renewable resources. They can be made into

various bio-products, including bamboo-based activated carbon. Based on preliminary

study of the characterisation of bamboo-based activated carbon from six different

bamboo species, we chose to develop few of them by both ex vitro and in vitro

propagations. In this study, bamboos were collected from private and government

nurseries. In the ex vitro propagation, induction of shoots was trialled to five different

bamboo species. The growth of shoot cuttings was observed in the media of husk

charcoal - coco peat. Meanwhile, initiation of the in vitro propagation was trialled to

three bamboo species, namely Gigantochloa robusta, Dendrocalamus asper and

Oxythenantera abbissinca. Two sterilisation methods were applied to sterile two types of

explant (i.e. young stem and shoot). Young shoots from sterile explants will then be

transferred into various combinations of induction medium for callus initiation, including

2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA),

indole-3-butyric acid (IBA) and cytokinins (kinetin). This research opens a possibility of

generating future bamboo with improved characteristic, in this case generating bamboo

with higher density of lignocellulose content.

Keywords : activated-carbon, callus, explant, ex vitro, in vitro, tissue culture

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26

Wood Quality of Kelempayan and Benuang from East Kalimantan,

Indonesia: Fiber Quality and Juvenile-Mature Boundary

Sari Delviana Marbun1, Imam Wahyudi

2*, Jajang Suryana

2, Deded Sarip Nawawi

2

1Science and Forest Products Technology Study Program, IPB University, Bogor, 16680,

Indonesia 2Department of Forest Products, Faculty of Forestry, IPB University, Bogor, 16680, Indonesia

*Corresponding author: [email protected]

Abstract. Scarcity of wood supply for wood industries in Indonesia is a serious problem.

Lesser-used wood species are potential to be alternative resources to overcome the scarcity

of wood supply. The aims of this research were to evaluate wood quality, mainly radial

variation of fiber quality and juvenile-mature boundary of two lesser-used wood species,

namely kelempayan and benuang. Fiber quality was analysed following Rachman and

Siagian‟s method. Radial variation of fiber and vessel length were used as parameters to

estimate juvenile-mature boundary using polynomial and segmented regression models.

The results showed that benuang fiber is classified as Quality Class I, while kelempayan

fiber is Quality Class II for pulp and paper manufacturing. In kelempayan wood, the

boundary of juvenility occurred in the 6 and 7th segments from the pith, while the whole of

benuang wood was still juvenile. Boundary between juvenile and mature wood on these

wood species was affected by parameter and method applied.

Keywords : benuang, fiber quality, juvenility, kelempayan, tropical rain forest

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27

The Effects of FeCl3 Concentration on Hydrothermal Pretreatment of Oil

Palm Fronds to Enhance Reducing Sugar Production

Nurwahdah1, Rodiansono

1, A Kamari

2, R Abdullah

3, Sunardi

1,4*

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Lambung

Mangkurat University, Banjarbaru 70714, Indonesia 2Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan

Idris, 35900, Tanjung Malim, Perak, Malaysia 3Department of Applied Physics, Faculty of Science, Universiti Brunei Darussalam, BE1410,

Brunei Darussalam 4Wetland-Based Materials Research Group, Lambung Mangkurat University, Banjarbaru

70714, Indonesia

*Corresponding author: [email protected]; [email protected]

Abstract. The effects of FeCl3 concentration on hydrothermal pretreatment of oil palm

fronds (OPF) to enhance reducing sugar production have been systematically investigated

for the first-ever. The hydrothermal pretreatment was carried out in autoclave reactor with

Teflon at 190 oC for 30 min with various FeCl3 concentration of 0.075; 0.150; and 0.225 M.

The residue from hydrothermal pretreatment (the noted as substrate) was hydrolyzed by

enzyme (Cellulase Onozuka RS) in water bath shaker at 50 °C for 48 h. The amount of

reducing sugar was analyzed by DNS (dinitrosalicylic acid) method using UV-visible

spectroscopy. The reduced mass of substrate, change of color, alteration of pH of filtrate,

and functional group analysis using Fourier Transform Infrared Spectroscopy (FTIR)

results indicate the decomposition of OPF structures. The highest reducing sugar (3.800

g/L) of the substrate was obtained when the concentration of 0.225 M was used, which is

higher than pretreatment of without FeCl3 addition (2.673 g/L). Overall, our study

concludes that hydrothermal pretreatment assisted by FeCl3 can catalyze the

decomposition of OPF structures to give accessibility of enzyme and enhance the reducing

sugar

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The potential for using corn husk as agricultural waste for particleboard

raw material

Kurnia Wiji Prasetiyo1*

, Lekianus Zalukhu2, Lilik Astari

1 and Dede Hermawan

2

1Research Center for Biomaterial Indonesian Institute of Sciences Jl.Raya Bogor Km. 46

Cibinong Bogor 2Department of Forest Product Technology Faculty of Forest and Natural Science Bogor

Agricultural University

*Corresponding author: [email protected]

Abstract. The development of particleboard manufactured using non wood raw materials

has become important due to the decreased of wood as main raw material for

particleboard industry. In this study, corn husk as agricultural waste was used for

experimental manufactured of particleboard bonded natural and synthetic adhesives. The

influence of adhesive types and concentration for physical and mechanical properties of

particleboard were investigated. The synthetic adhesive types used were urea

formaldehyde (UF) and phenol formaldehyde (PF) with 8,10 and 12 wt% adhesive

concentration. The natural adhesive used was citric acid with 15, 20 and 25 wt% adhesive

concentration. The target density of particleboard was set at 0.80 g/cm3. The boards were

manufactured at 130 oC for UF, 150 oC for PF and 200 oC for citric acid press

temperature, 10 minutes and 2.5 MPa for pressure of hot press. Generally, the adhesive

types and adhesive concentration were used in this study proven to influence the

properties of particleboard. The results showed that particleboard with citric acid

adhesive has better properties than particleboard bonded UF and PF adhesives.

Keywords: adhesive type, concentration, corn husk, particleboard, Sembilang bamboo

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The Effect Application of Organic Fertilizer and Dolomit Toward Cassava

Production in Banten Province-Indonesia

Viktor Siagian1*, Ulima Amanda

2, Eko Kardiyanto

3

1,2,3Assessment Institute for Agricultural Technology of Banten, Indonesia

Ciptayasa Avenue Km 01 Ciruas- Serang District, 42182. Telp. 0254-281055,

Fax 0254-282507.

* Corresponding author: [email protected]

Abstract. Cassava (Manihot Esculenta C.) is the third largest crop after rice, and corn in

Banten Province. The objectives of this study are: 1) Determine the effect of organic

fertilizer application and liming on cassava production in Lebak Regency, 2) Study the

difference of cassava farming on the demonstration plot with the cassava farming of

farmers. The research method uses a completely randomized experimental design (CRD)

on a 0.5 ha demonstration plot with 3 treatment plots each with 3 replications. Also with

the survey method by interviewing 11 cassava respondent farmers. The data analysis

method uses qualitative and quantitative analysis. Quantitative analysis uses the ANOVA

(Analysis of Variance) test and the qualitative test uses descriptive tabulations. The

results of this study are: 1) Fcounted = 180.36 > Ftable = 5,143, the conclution id that H0

is rejected, that there is at least one treatment applying organic fertilizer and or liming

which significantly affects the productivity of cassava. 2) The average productivity of

cassava on the demonstration plot is 42.13 tons of wet tubers / ha while at the farmer's

level is 24.18 tons of wet tubers/ha with B/C rasio is 4. The varieties planted are Manggu

Local Superior Varieties both on the demonstration plot and farmers.

Keywords: Cassava Farming, ANOVA Test, production, organic fertilizer, dolomit.

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30

Bioconversion of artemisinin by endophytic fungi associated Artemisia sp.

grown in Indonesia and Japan

Shoji Maehara1*, Ahmad Fathoni

2, Muhammad Ilyas

2, and Andria Agusta

2

1Faculty of pharmacy and pharmaceutical sciences,

Fukuyama University, 985, Sanzo, Higashimura-cho,

Fukuyama, Hiroshima, 729-0292, Japan 2Research Center for Biology, Indonesian Institute of Sciences,

Cibinong Science Center, Cibinong-Bogor 16911, Indonesia

*Corresponding author: [email protected]

Abstract. Artemisinin is a well-known anti-malarial drug that can be found in leaf of

Artemisia plant. The plant of Artemisia is growth widely in Europe, North America, North

Africa and Asia. Therefore, Artemisia plant that is called Wormwood or Mugword in

English, consist many plant species. The Indonesian mugwort or “baru cina”, and Japanese

mugwort or “Yomogi” has long been used as culinary herbs and medicinal plant. In this

report, we deal with symbiotic microorganisms in Artemisia plant grown in Indonesia and

Japan. Totally 15 endophytic fungi were isolated form Artemisia from Indonesia, 21

endophytic fungi from Artemisia from Japan. It was found that the composition of each

endophytic fungi varies depending on the growth environment (region). Isolated endophytic

fungi that promote the bioconversion reaction using artemisinin as a substrate have been

clarified by screening tests. Interestingly, it was found that the microbial conversion

reaction of each endophyte was different depending on the growth environment (region).

Here we show reactions by endophytic fungus DnAvCw-1 and Y13-2 associated Artemisia

plant grown in Indonesia and Japan. It was revealed that artemisinin was deoxygenated by

DnAvCw-1 and hydroxylated by Y13-2.

Keywords : artemisinin; endophytic fungi; bioconversion; anti-malaria; Artemisia

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Isolation of bioactive compounds from Hyptis suaveolens (L.) Poit. and

its antioxidant activity

Swe Swe Aye1*

, Khin Thant Sin2

1University of Yangon , Myanmar

2 Yangon University of Distance Education, Myanmar

*Corresponding author: [email protected]

Abstract. Hyptis suaveolens (Family – Lamiaceae) is a popular medicinal plant used

traditionally for antifertility, anti-inflammatory and antiplasmodial properties. So, This

study was designed to isolate bioactive compounds and to evaluate antioxidant activity of

isolated compounds from Hyptis suaveolens by silica gel column chromatographic

separation, gallic acid (10 .0 mg) was mainly isolated from Hyptis suaveolens.These

isolated compounds were structurally characterized by physico – chemical determination

and spectroscopic techniques such as UV and FTIR by comparing with the reported data..

Moreover, the essential oil was extracted from the fresh leaves of Hyptis suaveolens

by hydro-distillation method and analysed by GC-MS . It was found that

Caryophyllene ,and Sabinene were predominant compounds in essential oil of Hyptis

suaveolens . The antioxidant activity of isolated compounds and essential oil was

determined by DPPH free radical scavenging assay method. The results suggest that the

major compound gallic acid from H suaveolens possess the effective antioxidant

activity.

Keywords: Caryophyllene, free radical scavenging, gallic acid, GC-MS, Hyptis

suaveolens

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32

Cosmeceutical Potency of Functional Ripe Cider Buni

Sthefanie Marchellia, Raymond Rubianto Tjandrawinata, and Yanti*

Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta 12930,

Indonesia

*Corresponding author: [email protected]

Abstract. Buni fruit (Antidesma bunius) is a sour seasonal fruit in Indonesia, with limited

availability and only obtainable at certain area. Here, we formulated cider drink from ripe

buni fruits and determine its efficacy for cosmeceutical potency via antioxidant and

collagenase inhibitory activities. Buni ciders were fermented with both single and

combine cultures of Acetobacter xylinum and Saccharomyces cereviseae. Ciders were

further identified by gas chromatography-mass spectrometry (GC-MS) and tested for its

antioxidant activity by the 1,1- diphenyl-2-picrylhydrazyl (DPPH) radical inhibitory

assay, collagenase inhibitorory assay, alcohol content by Conway method, total soluble

phenolic content, and sensory analysis. Buni cider had pH 3.0 and alcohol content up to

8%. Compound profiling showed that buni ciders contained flavonoids, alkaloids, and

organic acids. The antioxidant activity of buni ciders was reached in range of 73-78% and

the total phenolic content in ciders was 7-20 µg gallic acid/mL. Buni cider fermented

from single culture had potential inhibitory effect against collagenase. Sensory profiling

showed that most panelists preferred to drink buni ciders than that of buni juice and

commercial cider. These data indicate that selected ripe buni cider with potential

antioxidant and collagenase inhibitory effects could be applied for cosmeceutical

application.

Keywords: antioxidant activity, cider drink, collagenase inhibitory effect,

cosmeceuticals, ripe buni fruits

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POSTER SESSION

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Diversity of Epipelic Diatom in Telaga Pengilon Dieng, Indonesia

Kenanga Sari1*

and Tri Retnaningsih Soeprobowati1,2

1Dept. Biology, Faculty Sciences and Mathematics Diponegoro Univeristy, Jl. Prof. Soedarto, SH,

Tembalang, Semarang, Indonesia 2School of Postgraduate Studies, Diponegoro Univeristy, Semarang 50241, Central Java, Indonesia

[email protected]

Abstract. Telaga Pengilon was affected by acid water with high amount of sulfur from

Telaga Warna, Telaga Pengilon is circumnetral water and has function as irrigation

sourcefor agriculture. the diversity diatom in location where the water moves through the

channel from Telaga Warna is unique. Both of lake are volcanic lake located in Dieng

plateau Indonesia in valley around Prau mountain with elevation 2086 masl, but has

different pHcharacteristic. The research about diatom composition and diversity in plateau

or high montain is adventage to know because the research still limited. Total 103 different

diatom taxa were identified, revealing a circumpolar taxa. Twenty eight taxa Eunotia are

found inthe aquatic ecosystem of Telaga Pengilon and The most dominant genera was

Eunotia formica and Eunotia tropica, Pinnularia was subdominant with 17 taxa. Eunotia

assemblagesoccurs in small acid water bodies indicated that Eunotia has wide range

tolerance for pHfluctuation.

Keyword: diatom, pengilon, diversity, eunotia, dieng

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35

Lipase Production by Paecilomyces sp. BK1 on Olive Oil

I Made Sudiana, Atit Kanti, Idris

Research Center for Biology, Indonesian Institute of Sciences, Indonesia

Abstract. Lipase is very important enzyme which catalyze several reaction include

esterification, transesterification, acidolysis and aminolysis. This enzyme are also

important for several industrial production. The objective of study was to evaluate the

effect of substrate, temperature and pH on lipase production under submerge fermentation.

Paecilomyces sp. BK1, Trichoderma viride BK2, Trichoderma sp. BK3, Aspergillus flavus

BK4, Fusarium oxysporum BK5, Paecilomyces chrysogenum BK6, Alternaria alternaria

BK7 were isolated from soil in palm oil plantation. Seven isolates were tested and analyses

was evaluated in tributyrin showing that Paecilomyces sp was the best isolates to produce

lipase. The isolates produce enzyme at pH 7 on 1 % olive oil content media after 72 hours

incubation. The enzyme was stable at temperature 30C until 50C. By the ability of

Paecilomyces to produce lipase and stable at wide range temperature imply that this strain

is potential for lipase production.

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36

Forest Management Prospects Through Development Non-Timber Forest

Products

Gusmailina1)

& Suwardi Sumadiwangsa2)

1)

Forest Products Research and Development Center 2)

Forest Products Research and Development Center Retired

*Corresponding author: [email protected]

Abstract. Non-timber forest products have long been known to be an important

component of the lives of communities around the forest. For most people, non-timber

forest products are an important resource compared to wood. Many households around

the forest area depend on their livelihood mainly on non-timber forest products as a

subsystem and as a main source of income. However, studies from the Worldwide Fund

for Nature (WWF) on NTFP efforts in several regions, show that the very large potential

of NTFPs carried out by communities around the forest is still a side business, even lower

than a side business, and has not been managed optimally. , so as to result in not being

able to contribute meaningful income to meet the needs of his family. Even though

non-timber forest products are prepared to be the new prima donna in the forestry

industry sector in the future. Therefore, the clustering of the forestry industry and the

pattern of partnership management become one of the main weapons to realize these

goals. This paper presents non-timber forest products and their prospects in the future.

Keywords : non-wood forest products, components, community, excellent

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37

Chemical Characterization of Wood Vinegar Obtained from Acacia Bark

Arief Heru Prianto1,2

, Budiawan1, Yoki Yulizar

1 and Partomuan Simanjuntak

3,4

1Department of Chemistry, Faculty of Natural Science and Mathematics, Universitas

Indonesia, . Kampus UI Depok.16424; Indonesia 2Research Center for Biomaterial-LIPI;

3 Research Center for Biotechnology-LIPI;

Jl. Raya Bogor km.46, Cibinong, Bogor 16911, Indonesia 4Faculty of Pharmacy, Universitas Pancasila,

Jl. Srengseng Sawah Jagakarsa, Jakarta Selatan 12640, Indonesia

Corresponding aurhors: [email protected]; [email protected]

Abstract. The chemical characteristics of wood vinegar produced from Acacia

mangium barks were done. The pyrolysis process was conducted into a low heating rate

400o C using a pyrolysis furnace. It was made of stainless steel, covered by fire clay

bricks, and used electrical heating. Acacia barks were pyrolyzed for 4.5 hours. The

organic acid and phenol were analyzed to determine the quality of wood vinegar. The

chemical components of wood vinegar were identified by gas chromatography-mass

spectrometry (GC-MS). The carbonation process produced charcoal, wood vinegar, gas,

and tar, i.e.56.0, 30.0, 11.1, and 2.93%, respectively. The major component of wood

vinegar were acetic acid (47.4%) and phenolic compounds (16.3%), while tar was

dominated by pentanoic acid (90.3%)

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38

Optimization of Pulp Concentration for Bioethanol Production from

Elephant Grass (Pennisetum purpureum) with Addition Tween 20 and

Using Two Commercial Yeasts

Ina Winarni1* and Dwi Wahyuni

2

1Forest Product Reseach and Development Center,

FORDA, Ciomas-Bogor 16610, Indonesia 2Faculty of Matemathics and Natural Scince, Pakuan University

Tegal lega, Bogor 16143, Indonesia

*Corresponding author: [email protected]

Abstract. The higher consumption of fuel, especially fossil fuels, will lead to depletion of

fuel reserves and high air pollution which causes environmental pollution. Therefore, it is

necessary to find alternative fuels that are environmentally friendly and abundant

availability in nature so called biofuels. One type of biofuel is bioethanol. Raw materials

that can be produced into bioethanol are biomass or lignocellulosic material. Elephant

grass is one type of lignocellulosic material which has high cellulose content (40.85%).

The objective of this study was to determine the optimum pulp concentration by adding

Tween 20 to the saccharification process with two types of commercial yeasts (fermipan

and mauripan) in the fermentation process. The pulp variations used are (20, 25 and 30%

dw) with Tween 20 concentrations (0% and 1% v). The results showed an increase in pulp

concentration and the addition of Tween 20 by 1% could increase the ethanol content

compared to controls (Without Tween 20). The highest ethanol content was produced at

30% pulp concentration (13.09%) with addition 1% of Tween 20 in the saccharification

process and fermipan yeast in the fermentation stage; and could achieved ethanol content

of 13.94%(v) when using Mauripan yeast.

Keywords : lignocellulose, elephant grass, bioethanol, fermipan, mauripan

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39

Two Chemotypes of Sindora leiocarpa Essential Oil and Evaluation of Its

Antimicrobial and Antioxidant Activity

Dewi Wulansari1, Kartika Dyah Palupi

1, Praptiwi

1, Hiroshi Kamitakahara

2, Toshiyuki

Takano2, and Andria Agusta

1*

1 Research Center for Biology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46,

Cibinong 16911, Indonesia 2 Graduate School of Agriculture, Div. Forest & Biomaterials Science, Kyoto University,

Japan

*Corresponding author: [email protected]

Abstract. Sindur oil is resinous wood oil from Sindur tree (Sindora leiocarpa). In Central

Kalimantan, Sindur oil is utilised to treat the wound and burn. This study aimed to reveal

the chemical constituent of Sindur essential oil (EO) and its antioxidant and antimicrobial

activity. The chemical components of the EOs were analysed by GC-MS method.

Antioxidant was evaluated using free radical DPPH, while antimicrobial assay was

performed using the broth microdilution method. Two chemotypes of Sindur EOs (A and

B) were successfully identified in which differ in its beta-cubebene, (-)-caryophyllene

oxide-type 1, and two unknown compounds content. Alpha-copaene was identified as a

major compound in both chemotypes. The two chemotypes of Sindur EO were tested

against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Bacillus

subtilis, and Candida albicans with range concentration of 0.098 – 12.5 %. Those two EOs

showed different activity which represented by MIC value. Type A EO was more active

against S. aureus with MIC of < 0.098 µg/ml, while type B was more active against S.

epidermidis and C. albicans with MICs value of < 0.098 and 6.25 µg/ml, respectively.

Both types of Sindur EOs did not show free radical DPPH scavenging activity at the

maximum concentration tested, 500 µg/ml.

Keywords : Sindur oil, Sindora, Sindora leiocarpa, antimicrobial

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40

SNAP (Single Nucleotide Amplified Polymorphism) Marker Development

Based on Eg-Le (Length) Gene for Early Identification of Low Height

Increment in Oil Palm

Annisa S. Salsabila1* Diny Dinarti1 Roberdi2 and Sudarsono

1

1Division of Plant Breeding and Biotechnology, Graduate School, Bogor Agricultural

University, Bogor-West Java 16680, Indonesia 2PT. SMART Tbk, Cijayanti, Babakan Madang, Bogor-West Java 16810, Indonesia

*Corresponding author: [email protected]

Abstract. Controlling plant height increment of oil palm is one of the strategies to increase

the productivity and slow down the oil palm plantation expansion. The 3β- hydroxylase

(GA3ox) encoded by the LE gene is one of the enzymes involved in the final stage of

Gibberellin biosynthesis. In a pea, plant carrying the mutant of le gene was as short as 40%

of the wild-type one. This research aimed at identifying and determining SNP of Eg-LE

gene and used the identified variability to generate functional SNAP markers. Fragments

of the LE gene were amplified from E. Guineensis (Eg), E. oleifera (Eo) and the Eo x Eg

interspecific hybrids. The PCR amplified products were sequenced and SNAP primer sets

were developed from the identified SNPs using WebSNAPER software. Selected primers

then evaluated for an allele-specific amplification. Results of the sequencing identified a

total of 11 Single Nucleotide Polymorphism (SNP) positions within the coding sequences

of the Eg-LE gene. Eleven SNAP primer sets were tested against 69 oil palm genotypes.

The one SNAP marker based on 1008th loci were associated with the low plant height

increment character in the adult oil palm. Therefore it could be used to support in oil palm

breeding program.

Keywords : dwarf, ga3ox, gibberellin biosynthesis, snap marker, oil palm selection

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41

Diversity Of Stands Type At Buffer Zone Karst Citatah

Muhamad Yusup Hidayat

1Center for Research and Development of Quality and Environment Laboratory, Ministry of

Environment dan Forestry, Serpong-South Tangerang 15314, Indonesia

*Corresponding author: yusup. [email protected]

Abstract. Karst area is an ecosystem type that has one important function as water

conservation. It is important to know the composition and structure of the stand of karst

area. This study aims to determine the diversity of stands and their potential in Citatah karst

buffer zone. Data of stands composition were collected by using plot purposively on public

forest land owned by the community, spread in three villages. Vegetation analysis was

conducted by census (100%) in all selected stand areas. Index of Importance Value (INP)

showed that Sengon stands (Paraserianthes falcataria) were dominant, both of

regeneration level (79,23) and tree level (165,71). Index of Diversity of the regeneration

level is very stable as it has a range of H '> 2 compared to the tree level that has a range of

H' value 1-2. Index of dominance is only seen at the tree level, That is the Sengon

(Paraserianthes falcataria) stands, C value is one. At the level of regeneration, there is no

visibility of domination one species to other species. Index of similarity at regeneration

level and tree level is quite high (> 55%). Regeneration at the regeneration level grows well

until it reaches the tree level. The pattern of management that suitable acording to the

vegetation structure in the research area is public forest with mixed garden system.

Keywords : Karst Area, Index of Importance Value, Index of diversity, Index of

domination and index of simialirity, public forest

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42

Phytochemical Content and Antioxidant Activities of Medicinal Plants from

North Sumatra

Gunawan Pasaribu1* and Totok K. Waluyo

2

1,2

Forest Product Research and Development Center,

Jl Gunung Batu No 5 Bogor , Indonesia

*Corresponding author: [email protected]

Abstract. This paper is based on ethnopharmacological investigation in KPHL XXII

Tobasa Labura, North Sumatra. The method of study is conducted through indeept

interviews on local people. The phytochemical compound was qualitatively examined in

the methanol crude extract included flavonoid, tannin, saponin, triterpenoid, steriod,

hydroquinon and alkaloid. The antioxidant activity of the extract was based on the

scavenging activity of the stable 1, 1-diphenyl-2-picrylhydrazyl(DPPH) free radical. There

are ten important species of medicinal plant have collected. The local people used the

medicinal plant of various disease or disorder such as gastrointestinal disorder,

antidiabetic, wound healing, fever, stomach ache and antiinflamation. The phytochemical

testing showed that the extract contains flavonoid, saponin, tanin and alkaloid. The

antioxidant activity (IC50) showed that Sikkam 27,06 ppm; Modang Kulim 104,81 ppm;

Handis 35,38 ppm; Rugi-rugi 45,70 ppm; Sambang-sambang 207,63 ppm; Tolong 58,97

ppm; Sae-sae putih 53,19 ppm; Sae-sae hitam 41,08 ppm; Pirdot 146,40 ppm and

Sirungguk more than 250 ppm. In general, the antioxidant activity of ten medicinal plant

species studied had good activity.

Keywords : Antioxidant, ethnopharmacology, plant, pyhtochemical, Sumatra

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43

The Comparison of different diatom digestive method using HCl, H2O2 and

HCl, KMNO4 in Telaga Pengilon Dieng

Christopher Hardian Widyanta1*

and Tri Retnaningsih Soeprobowati1,2

1Dept. Biology, Faculty Science and Mathematics Diponegoro University, Jl. Prof. Soedarto,

SH, Tembalang, Semarang, Indonesia 2School of Postgraduated Studies, Diponegoro University, Semarang50241, Central Java,

Indonesia

[email protected]

Abstract. Identification diatom has been applied as an important key to trace

paleoenvirontmental condition. The knowledge for diatom extraction from sediment is

still continue, but, the diatom digestive methods in tropical area is still limited. Telaga

Pengilon is located in Dieng Indonesia rich of organic material in the sediment, the

sample also associate with epifitic and benthic diatom. Therefore, extracting pure diatom

frustules free of organic matter from the sediments is an essential requirement for this

application. To find the appropriate method for extracting diatoms in Telaga Pengilon,

this research compares HCl with H2O2 and HCl with KMNO4 as the digestive reagent.

Based on statistic result (HCL, KMNO4) less time consumed than (HCL, H202) during

digestive process (p<0.05) and produced same amount residu (p> 0.05). For Pinnularia

and Frustulia , the majority of diatom could be extracted using both of methods. Under

microscopy, the structure of diatoms remained almost perfect after digestion with H202,

the striae clearly visible and the residu digest perfectly. Another using HCL,KMNO4

fragmentation was found for some Pinnularia and for Frustulia the striae is clearly visible.

This study demonstrated that different diatom has different resistance for reagent and

some of location need different regent because has different material contain in the

sediment and need help to remove material organic. As far as the HCL, and H2O2 is

appropiate methods for digestive in tropical area such as Telaga Pengilon, HCL,KMN4

can be substitute for less time consuming process.

Keyword: Diatom, digest, methods, HCL, KMNO4, H2O2

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44

PE Goat Performance in the Breed Stock Area, Banjarmangu District,

Banjarnegara Regency, Central Java Province

Tatan Kostaman1*, Angga Ardhati Rani Hapsari

1, Singgih Setiawan

2, Bess Tiesnamurti

2, and

Anneke Anggraeni1

1Indonesian Research Institute for Animal Production (IRIAP),

Jl Veteran III Banjarwaru, Ciawi-Bogor 16720, Indonesia 2Indonesian Center for Animal Research and Development (ICARD),

Jl. Raya Pajajaran Kav E-59, Bogor 16151, Indonesia

*Corresponding author: [email protected]

Abstract. Banjarmangu Subdistrict, Banjarnegara District, Central Java Province has been

designated as a breeding stock for PE goats. The purpose of the study was to determine the

performance of PE goats in the breeding area. The study was conducted using a survey

method, interviews with goat breeders about reproductive characteristics, number of

ownership, and livestock management accompanied by observations to the breeder's

location. The respondents interviewed were 24 people. Criteria for determining

respondents are livestock farmers who have original PE goats. The results showed that the

current population averages based on the sex ratio of male and female adults are not ideal

which is 2:3. Meanwhile, the sex ratio of the male and female kids which is 1:1. The mating

management is still carried out naturally, that is female animals that are being lured

together with superior males. The selection system is carried out by farmers based on the

quality of the kids produced. The maintenance system is carried out intensively.

Management of animal health is handled by animal health workers together with breeders

and the disease that often arises is scabies. It was concluded that the performance of PE

goats in the seed source area needs to be improved in quality, namely by the introduction of

technology.

Keywords : PE goats, performance, breed stock

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45

Screening Phytochemical Compound of Alstonia scholaris R.Br in Different

Sites in Indonesia

Zuraida1*

and Sulistiyani2

1Forest Research and Development Centre, Environment & Forestry Research Development

and Innovation Agency (FORDA), Ministry of Environment and Forestry, Jalan Guning Batu

no 5 Bogor 16610, Indonesia 2Biochemistry Department, IPB University Bogor, Jl. Raya Dramaga, Bogor 16680

*Corresponding author: [email protected]

Abstract. Indonesia's tropical forest is widely used as a source of raw materials of

traditional medicine. Pulai (Alstonia scholaris R.Br) is one of medicinal plants widely

spread in Indonesia to treat various diseases. Phytochemical compounds play important

role in farmacology effect, which depend on many factors such as age, geographical site,

solvent and extraction methods.These factors result in different phytochemical

compounds, and biological activity. The aimed of this research was to investigate

phytochemical content of Alstonia scholaris’ bark from different region in Indonesia by

maceration and reflux extraction methode. Alstonia scholaris bark‟s extract were tested its

phytochemical content for alkoloids, flavonoids, tannins, steroid saponins and

triterpenoids. The results showed that pulai bark extracted by maceration was bigger in

yield than reflux method. Phytochemical tested showed that all samples positive

flavonoids, and alkaloids. Alstonia scholaris’ bark from Cifor-Bogor had the strongest

flavonoids content of all. Flavonoid and alkaloid aree phytochemical compound play

important role as antioxidant used for anti aging, aterosklerosis, diabetes, and

inflammation.

Keywords : Alstonia sp.; flavonoids; maceration; phytocemical; reflux

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46

Current Status and Species Diversity of Seagrass on Panjang Island,

Banten

Yayuk Sugianti1*

and Mujiyanto1

Research Institute for Fish Resources Enhancement

Jalan Cilalawi No. 1 Jatiluhur, Purwakarta, Indonesia

*Corresponding author : [email protected]

Abstract. Damage to seagrass beds on Panjang Island, Banten, has increased every year.

The most significant decline occurred at an interval of 2000-2005 with a decrease of

about 22.9 ha. Seagrass damage continued to increase at the year between 1989-2002 as a

result of natural stone mining and coastal reclamation activities to become industrial

areas and ports. The objective of this study was to determine the characteristics of the

species and current status of seagrass communities in Panjang Island waters, Banten. The

study was conducted on October 2015 and April 2016 located in Panjang Island waters,

Banten. Analysis of seagrass data included identification of species, frequency of seagrass

species, calculating the density, percentage of the seagrass coverage, and important value

index. Based on these results, it can be seen the level of current seagrass conditions. PCA

analysis was also conducted to determine the relationship of seagrass density with

chemical physics parameters. The results obtained showed the condition of seagrass

ecosystems in Panjang Island waters, Banten, which was at a level of moderate to severe

damage. The results of PCA analysis showed that water temperature, nitrate concentration,

DO, and TSS greatly influenced the density of seagrass in these waters.

Keywords: Banten, current status, seagrass, species diversity, Panjang Island

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47

Effect of Coconut Water and Extract of Banana Hump on The Growth of

Binahong (Anredera cordifolia (Ten) Steenis) Accessions from the

Lowland

Yaya Hasanah*1)

, Lisa Mawarni1)

, Chairani Hanum1)

, Nurhaida

1)

1)

Faculty of Agriculture, Universitas Sumatera Utara

Jl. Prof. A. Sofyan No 3 Kampus USU, Medan 20155 Indonesia

*Corresponding author : E-mail : [email protected]

Abstract. Application of natural plant growth regulators (PGR) influences the growth of

binahong. The objective of the research is to evaluate the growth component of lowland

binahong accessions due to the application of natural PGR of coconut water and banana

hump extract. The research used factorial randomized block design with 2 factors and 3

replications. The first factor is accession of binahong from lowlands (Marelan; Ampera

Tmur ; Helvetia) in Medan. The second factor is the application of natural PGR, namely

without the application of PGR ; 40% coconut water application; banana hump extract

40% ; coconut water 40% + banana hump extract 40%. The results showed that the

accessions from Ampera Timur had plant length, shoot dry weight and root dry weight that

were higher than the other accessions. The treatment of 40% coconut water + 40% coconut

water extract increased plant length, and root dry weight. Interaction of Helvetia binahong

accession treatment and 40% coconut water + banana hump extract 40% increased plant

length of binahong.

Keywords : binahong, accesion, coconut water, banana hump

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48

Evaluation of The Potency of Endophytic Fungi Associated with Artemisia

annua and Artemisia vulgaris as Antibacterial and Antioxidant

Evana1, Praptiwi

1, Ahmad Fathoni

1, Muhammad Ilyas

1, Shoji Maehara

2 and Andria Agusta

1*

1Research Center for Biology, Indonesian Institute of Sciences,

Cibinong Science Center, Cibinong-Bogor 16911, Indonesia 2Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University,

Sanzo, 1, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan

*Corresponding author: [email protected]

Abstract. Artemisia annua and Artemisia vulgaris have been known as traditional herbal

medicine. The purpose of this study was to evaluate the potential of 8 endophytic fungi

associated with A. annua, and 16 endophytic fungi associated with A. vulgaris as

antibacterial and antioxidant. Preliminary screening for antibacterial and antioxidant

activities were carried out by the TLC-bioautographic method, and the active extracts were

further analyzed for their MIC and IC50 values by serial microdilution method at 96

microwell plates. The results showed that 23 endophytic fungi were able to inhibit

Staphylococcus aureus with MIC values ranging from 64 to > 256 µg/ml, 20 endophytic

fungi inhibit the growth of Escherichia coli with MIC values of 128 to > 256 µg/ml. Two

isolates have very strong antibacterial activity against S. aureus (MIC = 64 µg/ml) were

DnAvCb-3 and TdAaCb-2. Sixteen endophytic fungi extracts were active as antioxidant by

acting as DPPH free radical scavengers. The results of this study indicated that the

endophytic fungi extracts associated with A. annua and A. vulgaris were promising natural

sources for antibacterial and antioxidant.

Keywords : Artemisia annua, Artemisia vulgaris, endophytic fungi, antibacterial, DPPH

antioxidant.

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Distribution of Long-Tailed Monkeys (Macaca fascicularis) in Kelimutu

National Park

Ridwan Fauzi1,2

*, Tonny Wuryanto1,3

, Endarto1,4

, Fendra Suarmadi1, and Albertus Tomonob

1

1 Kelimutu National Park, El Tari Street No. 16, Ende-Flores, NTT

2 Research and Development Center for Environmental Quality and Laboratory, Ministry of

Environment and Forestry, Kawasan Puspiptek Gedung 210, Serpong, Tangerang Selatan 3 Directorate for Management and Information on Nature Conservation, Ministry of

Environment and Forestry, Jl. Ir Juanda No 15 Bogor 4Baluran National Park, Jl. Raya Situbondo Banyuwangi KM.35, Kec. Banyuputih, Kab.

Situbondo, Jawa Timur

*Corresponding author: [email protected]

Abstract. Long-tailed monkey (Macaca fascicularis) is one of mammal that lives in

Kelimutu National Park (Kelimutu NP). This mammal in their natural habitat has an impact

on buffer zones in the form of animal activities that damage crops on plantation lands. The

purpose of this study was to identify the distribution of long-tailed monkey populations.

This research was conducted in August 2010 by covering areas within the region and

outside the Kelimutu NP area. Data analysis was used to calculate the abundance index, the

encounter rate for estimating abundance and distribution based on the length of the track

surveyed. The results indicates that the distribution of long-tailed monkey populations in

Kelimutu NP was most widely found in field habitats, with relatively low abundance of

around 5 individuals/ km2. Based on the calculation of habitat carrying capacity for groups

of monkeys in the tourism zones, it is still below the maximum limit. Increased awareness

of ecological insight tourism also needs to be optimized related to the presence of monkeys

in the tourism zones of Kelimutu NP. Development of habitats, such as planting of trees for

foods and protected zones is necessary to provide suitable habitat for monkeys.

Keywords : Macaca fascicularis, Kelimutu National Park, protected zones

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Toward 2025 Sustainable use of Biodiversity

50

Local Rice Conservation Through the Local Wisdom of Indigenous Culture

at Kasepuhan Cisungsang Banten Province

Pepi Nur Susilawati, Zuraida Yursak and Ulima D Amanda

Peneliti Balai Pengkajian Teknologi Pertanian Banten

Jl. Ciptayasa KM.01 Ciruas –Serang Provinsi Banten

Email : [email protected]

Abstract. Local wisdom is very important in conservation of local rice. Utilization of

local rice varieties has been carried out in the specific groups of people for centuries

under local culture rules (Kasepuhan Cisungsang). Cisungsang culture is one of the local

cultures in Banten Kidul with indigenous people who still maintain local wisdom in rice

cultivation. The research objective is to observe the role of Cisungsang indigenous

culture in preserving local rice germplasm. The study was conducted in Kasepuhan

Cisungsang, Cibeber District, Lebak Regency, Banten Province. The data used were

primary and secondary data, with survey and desk study. The results showed that the law

of Cisungsang culture applies not only to people and community life but also to

agriculture especially for rice cultivation. Rice, manifested as goddess “Dewi Sri”, has a

noble position for indigenous people as it must be properly nurtured and nourished in

order to provide optimal harvest. There were 30 varieties of local rice varieties developed

in Kasepuhan Cisungsang and surrounding areas, but only 10 varieties were planted by

the community: Marileun, Terong Papak, Cireh Gudang, Kapundung, Tampai Beureum,

Srikuning, Tampey Bodas, Nemol, Gadog, Angsana. The ten varieties were in accordance

with the farmer preferences and adaptive strength in the local agroecosystem.

Keywords: upland rice, local wisdom, kasepuhan cisungsang, Banten Kidul

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51

In vitro response of patchouli in a medium by addition of

Methylobacterium spp filtrate

Mia Kosmiatin1* and Seli Salma

2

1Indonesia Center of Agricultural of Biotechnology and Genetic Resources Research and

Development, ICABIOGRAD, Jalan Tentara Pelajar No 3A, Bogor, 16111, Indonesian 2Indonesia Soil Research Institute, Jalan Tentara Pelajar No 12, Bogor 16114, Indonesia

*Corresponding author: [email protected]

Abstract. Mass providing superior varieties, are become important to support modern

farming. Propagation by in vitro technique could be solving that problem, but the price

determined by medium formulation. The addition of synthetic PGR has been a residual

effect, meanwhile natural PGR more secure and expensive than the synthetic ones. In

vitro testing of PGR activity obtained from bacteria was be the first step to substituted

synthetic PGR to become nature PGR. Research to observe PGR activity obtained from 4

strains of Methylobacterium spp from East Kalimantan that had been analyzed of the

capability of PGR synthesis was be done on in vitro patchouli cultured. There are 3

activities: 1. The sterilized technique of Methylobacterium spp filtrate; 2) Testing of

Methylobacterium spp from that production of cytokinin; and 3) auxin. The research

showed that strain TD-J2 filtrate added to medium had been similar and better to cytokinin

activity and strain TD-J7similar to zeatin activities to callus induction and could be shoot

regenerated. Auxin activity derived from strain TD-J10 showed the capacity to induces

root formed. The research result indicates that synthesis PGR derived from

Methylobacterium spp. has similar activity with synthetic PGR which commonly used in

plant in vitro culture.

Keywords: Patchouli, in vitro culture, Methylobacterium spp, plant growth regulators

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52

Legislation on genetically modified animals and its application possibility in

Indonesia

M. Ikhsan Shiddieqy1*, Bess Tiesnamurti

1 and Sri Muharsini

2

1Indonesian Center for Animal Research and Development

Jalan Pajajaran Kav E59, Bogor 16151, Indonesia 2Indonesian Research Center for Veterinary Sciences

Jalan RE Martadinata No. 30 PO BOX 151, Bogor 16114, Indonesia

*Corresponding author: [email protected]

Abstract. The government is responsible for fulfil the needs of safe, healthy and halal food

for all its people in a sustainable manner. The world population will increase from 7 to 9

billion people in 2050. While the availability of land may not increase in the future, it may

even not used for agriculture. Scientists are trying to overcome this problem by creating

technologies that can increase livestock productivity, one of which is through a genetically

modified process. Genetically modified animals are animals that have been genetically

modified for a many of purposes including yields and disease resistance. Although

genetically modified animals are at the research stage and the possibility to entering

Indonesia remains small, but government should prepare the regulations related to it.

European countries and USA have regulations to control genetically modified animals.

Malaysia also has similar regulation since 2007. Currently, Indonesia has formed an

authority that oversees the genetically modified product, namely Biosafety Commission

(Komisi Keamanan Hayati). However, this commission only supervises genetically

modified products in food and feed. This paper provides general review on regulations

related to genetically modified products and detailed suggestions on regulation regarding

genetically modified animals

Keywords : Genetically modified products, animals, regulation

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53

Optimization of Xylose Production from Sugarcane Trash by

Microwave-Maleic Acid Hydrolysis

Euis Hermiati1, Maulida Oktaviani

1, Riksfardini Annisa Ermawar

1, Raden Permana Budi

Laksana1, Lutfi Nia Kholida

2, Ahmad Thontowi

2, and Siti Mardiana

3

1Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor

Km 46, Cibinong, Bogor 16911, Indonesia 2Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor

Km 46, Cibinong, Bogor 16911, Indonesia 3University of Lampung, Jl. Prof. Dr. Sumantri Brojonegoro No. 1, Bandar Lampung 35145,

Indonesia

*Corresponding author: [email protected]

Abstract. Sugarcane trash contains significant amount of xylan that could be hydrolysed to

xylose. The xylose could be further fermented to produce xylitol, a sugar alcohol that has

low calories and does not cause carries of teeth. In this study we optimized the production

of xylose from sugarcane trash by microwave-assisted maleic acid hydrolysis using

response surface methodology (RSM). The factors optimized were acid concentration,

time, and temperature. The xylose concentration obtained was determined and it served as

a response variable. Results show that acid concentration has significant effect on xylose

production, and the model generated from the optimization was fit and can be used to

predict the xylose concentration in the hydrolysate with various combinations of acid

concentration, time, and temperature. The optimum condition for xylose production from

sugarcane trash was using maleic acid of 1.7%, and heating at 177 °C for 6.7 min. At this

condition there was 25 g/L of xylose could be produced.

Keywords : Maleic acid, microwave heating, response surface methodology, sugarcane

trash, xylose

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54

Estimation of Biodiesel Production from Used Cooking Oil of University

Canteen to Support Sustainable Electricity in Universitas Pertamina

Ari Rahman1*, I Wayan Koko Suryawan

1, Ariyanti Sarwono

1, Nurulbaiti Listyendah Zahra

1,

Ziyadatulkhair M. Faruqi2

1Faculty of Infrastructure Planning, Department of Environmental Engineering, Universitas

Pertamina, Komplek Universitas Pertamina, Jalan Sinabung II, Terusan Simprug, Jakarta

12220, Indonesia 2Undergraduate Program, Department of Environmental Engineering, Universitas Pertamina,

Komplek Universitas Pertamina, Jalan Sinabung II, Terusan Simprug, Jakarta 12220,

Indonesia

*Corresponding author: [email protected]

Abstract. Universitas Pertamina is a new private university founded in 2016. The

university has a vision to be a world-class university leading in the energy sector. In

achieving its vision, the university began to carry out various programs related to energy.

One such program that is being promoted is the use of renewable energy resources. The

objective of this study was to investigate the used cooking oil, obtained from the university

canteen as an alternative feedstock for producing biodiesel fuel. Biodiesel is produced

through a chemical process called transesterification. Based on the survey questionnaire,

the university canteen produces on average 36 L/day of used cooking oil. The amount of

biodiesel produced was 30.2 L/days with 83.9% of yield. Using a diesel testing machine,

biodiesel with B30 type (30% biodiesel and 70% diesel in volume) holds a potential to

generate electricity of 3,021.2 KWh/month. This value able to contribute 12% to lighting

the Rectorate Building for university administration and official activities. Savings of Rp.

3,348,857 can be achieved every month by substituting diesel fuel for biodiesel. Moreover,

by considering biomass as sustainable renewable energy resources, a reduction in carbon

emissions can be valued by 2.2 MTCO2/month.

Keywords: Biodiesel, Carbon emission, Electricity, Universitas Pertamina, Used cooking

oil

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Toward 2025 Sustainable use of Biodiversity

55

The Effect of Sucrose Concentrations and Shoot Inoculum Density on The

Growth of In Vitro Shoot of Teak (Tectona Grandis L.) **

Witjaksono*, Indira Riastiwi, Katarina Utami Nugraheni dan Gut Windarsih1

Plant Cell Tissue Culture Laboratory, Botany Devision, Research Center for Biology,

Indonesian Institute of Sciences, Jl. Raya Bogor Jakarta Km 46, Cibinong, Bogor 16911

1Universitas Islam Negeri Sultan Maulana Hasanuddin,

Jl. Syech Nawawi Al Bantani Andamui, Curug, Serang 42116, Banten

*Corresponding author: [email protected]; [email protected]

**All authors contributed equally to this work

Abstract. The teak belongs to the highly prized timber tree. Quality teak trees have been

guaranteed by planting clonal planting materials propagated vegetatively, for example by

shoot tip cutting and tissue culture. Tissue culture propagation of teak has been

commercially viable, eventhough efficiency of production can still be improved. This

research aims to study the effect of increasing shoot inoculum density and sucrose

concentration requirement for optimum growth of teak shoot in vitro. Inocula of a single

nodal shoot in the number of 5 or 9 were inoculated on modified MS basal media with

sucrose concentrations treatment of 20, 30, 40, 50 and 60 g l-1. The results showed that

standard sucrose concentration of 30 g l-1 was optimum for the growth five inocula,

while for nine inocula, the optimum sucrose concentration was 40 g l-1. Eventhough the

average shoot growth was lower for nine inocula than that of five inocula, the total shoot

growth was higher for the former. Increasing number of inocula can be used as a method

to increase the efficiency of shoot proliferation.

Keywords: teak, micropropagation, in vitro, sucrose, inoculum density, shoot culture

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56

Screening of Antibacterial and Antioxidant Activities of Stingless Bee

(Tetragonula laeviceps Smith) Extracts Collected from Purwodadi Botanical

Garden – East Java, Indonesia

Oscar Efendy1,2*

, Ahmad Fathoni1, Praptiwi

1, Dewi Wulansari

1, Wibowo Mangunwardoyo

2,

and Andria Agusta1

1Research Center for Biology, Indonesian Institute of Sciences, Jl. Raya Jakarta – Bogor KM

46, Cibinong 16911 2Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Indonesia,

Campus UI Depok, West Java 16425, Indonesia

*PhD student, Corresponding author: [email protected]

Abstract. This study was conducted to determine the potential of Tetragonula laeviceps

methanol extract and fractions (hexane, dichloromethane, ethyl acetate, methanol, and

acetate-water) as an antibacterial, antioxidant and β-glucosidase inhibitor. The test was

carried out by thin layer chromatography (TLC) - bioautography (dot-blot and elution).

Antibacterial tests were carried out against Staphylococcus aureus and Escherichia coli,

antioxidant potential was carried out by DPPH method. The results showed crude extract,

hexane and dichloromethane fractions had moderate antibacterial activity against S. aureus

(MIC values: 128-256 ug/ml) tetapi weak antibacterial activity against E.coli. The extract

and fractions of dichloromethane, methanol and aceton-air had very strong antioxidant

activity, while dichloromethane and ethyl acetate extracts had activity as β-glucosidase

inhibitors. The results showed that the klanceng hive extract (T. laeviceps) had bioactivity

as an inhibitor of β-glucosidase, antibacterial and antioxidant. Further research to

determine the potential and isolation of active compounds needs to be done. So, it can be

explored further as new sources of antioxidant, antibacterial and β- glucosidase inhibitor.

Keywords: Stingless bee, TLC-bioautography, antibacterial, antioxidant

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57

Diversity of The Ecletus Parrot (Ecletus roratus) Birds Inferred From

Mitochondraial Gene Sequences

Dwi Astuti

Research Center for Biology, Indonesian Institute of Sciences

Gd. Widyasatwaloka, Jl. Jakarta – Bogor KM 49, Cibinong Science Centre, Cibinong West

Java.

e-mail : [email protected]

Abstract. Electus parrot (Eclectus roratus) Bird is included in the group of parrots, the

Psittacidae family. Body size is 35-42 cm; and weighs is 355–615 g. Eclectus bird have 9

subspecies. Their distribution is in the Solomon Islands, Sumba, New Guinea and nearby

islands, northeastern Australia, and the Maluku Islands (Moluccas). Seven of these

subspecies are in the territory of Indonesia. Wild populations is in declining with the

Least Concern category on the IUCN Red List and included in the CITES Appendix. Like

other parrot birds, this bird has high economic value so its existence needs to be

preserved for used sustainably. The study of the genetic diversity of these birds was

revealed in this study based on DNA sequences of mitochondrial cytB gene. This

information might be needed for their conservation efforts. Blood samples of 0.1 cc were

taken from 13 birds in the zoo and in captivity. DNAs were extracted from each blood

sample and then DNA fragments from the mitochondrial cyt b gene through a PCR

process and then the nucletide was sequenced. DNA sequence data from the cytb gene

were analyzed using specific software to get information about variations, genetic

diversity and grouping of individual birds. Of the 720 cyt-b base pairs there were no stop

condons, there were 7 base positions that vary starting at 112 base site and ending at 616

base side. Genetic distance between individual birds ranges from 0,000 to 0.012. There

were 3 sequence haplotypes with a haplotype diverity (Hd) value of 0.500 ± 0.136 and

Nucleotyde diversity (Pi) value of 0.004431 ± 0.00140, average number of nucleotide

differences (k) 2. 615.

Keywords: Eclectus Parrot Bird, cytB sequences, nucleotide variation, genetic

distance, haplotype n nucleotyde diversities

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58

Distribution of Long-Tailed Monkeys (Macaca fascicularis) in Kelimutu

National Park

Ridwan Fauzi1,2

*, Tonny Wuryanto1,3

, Endarto1,4

, Fendra Suarmadi1, and Albertus Tomonob

1

1 Kelimutu National Park, El Tari Street No. 16, Ende-Flores, NTT

2 Center for Research and Development of Quality and Environmental Laboratory, Ministry of

Environment and Forestry, Kawasan Puspiptek Gedung 210, Serpong, Tangerang Selatan 3 Directorate of Designing and Information on Nature Conservation, Ministry of Environment

and Forestry, Jl. Ir Juanda No 15 Bogor 4Baluran National Park, Jl. Raya Situbondo Banyuwangi KM.35, Kec. Banyuputih, Kab.

Situbondo, Jawa Timur

*Corresponding author: [email protected]

Abstract. Long-tailed monkey (Macaca fascicularis) is one of mammal that lives in

Kelimutu National Park (Kelimutu NP). This mammal in their natural habitat has an impact

on buffer zones in the form of animal activities that damage crops on plantation lands. The

purpose of this study was to identify the distribution of long-tailed monkey populations.

This research was conducted in August 2010 by covering areas within the region and

outside the Kelimutu NP area. Data analysis was used to calculate the abundance index, the

encounter rate for estimating abundance and distribution based on the length of the track

surveyed. The results indicates that the distribution of long-tailed monkey populations in

Kelimutu NP was most widely found in field habitats, with relatively low abundance of

around 5 individuals/ km2. Based on the calculation of habitat carrying capacity for groups

of monkeys in the tourism zones, it is still below the maximum limit. Increased awareness

of ecological insight tourism also needs to be optimized related to the presence of monkeys

in the tourism zones of Kelimutu NP. Development of habitats, such as planting of trees for

foods and protected zones is necessary to provide suitable habitat for monkeys.

Keywords : Macaca fascicularis, Kelimutu National Park, protected zones

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59

Classification of Agarwood Product from Riau – Indonesia using Near

Infrared Spectroscopy

Danang Sudarwoko Adi1*, Dwi Ajias Pramasari

1, Bernadeta Ayu W

1, Adik Bahanawan

1,

Yusup Amin1, Sukma Surya K

1, Wahyu Dwianto

1, Hwang Sung-Wook

2 and Junji

Sugiyama2,3

1Research Center for Biomaterials, Indonesian Institute of Sciences,

Cibinong Science Center, Cibinong-Bogor 16911, Indonesia 2Research Institute for Sustainable Humanosphere, Kyoto University

Gokasho, Uji, Kyoto 611-0011, Japan 3College of Materials Science and Engineering, Nanjing Forestry University

Nanjing 210037, China

*Corresponding author: [email protected]

Abstract. Up to know, classification of agarwood product in Indonesia use visualization of

surface colour as well as the odour level, thus the results are qualitative. The aim of this

study is to investigate the potential utilization of Near Infrared (NIR) spectra in

identification of the agarwood products. Five different grades of agarwood from Riau –

Indonesia were used in this experiment. They were subjected to the NIR instrument at

wave number 10,000 – 4,000 cm-1

. Principal component analysis (PCA) and several

classifier, such as k-nearest neighbour (k-NN) and support vector machine (SVM) were

used to analyse the data. Furthermore, random forest was also used to obtain the feature

importance for the classification. All of the program were performed on Python 3.7.3. The

results revealed that all sample were clear distributed on the different cluster of PCA score

plots. The accuracy of k-NN and SVM were also very high and well separated among five

grades agarwood. The application of NIR spectra on agarwood is promising to generalize

the identification of different graded products. However, taking more variation of the

samples to build a data base is suggested to make the classification more accurate and

reliable.

Keywords : agarwood; PCA; classification, NIR

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60

Identification and Fiber Morphology Measurements of Enggano Island

Woods Species

Wahyu Dwianto1*, Danang S. Adi

1, Teguh Darmawan, Adik Bahanawan, Eka Lestari

1, Yusup

Amin1, Dwi A. Pramasari

1, Fitria, Ratih Damayanti

2, Sri Rulliaty

2, Junji Sugiyama

3

1Research Center for Biomaterials, Indonesian Institute of Sciences,

Cibinong Science Center, Bogor 16911, Indonesia 2Forest Products Research and Development Center, Ministry of Environment and Forestry,

Gunung Batu, Bogor 16610, Indonesia 3Research Institute for Sustainable Humanosphere, Kyoto University

Gokasho, Uji, Kyoto 611-0011, Japan

*Corresponding author: [email protected]

Abstract. The objectives of this exploration were to find, identify and characterize the

potential of lesser-known wood species in Enggano Island, especially for sawn-timbers.

Wood samples were taken by drilling the trees to get a pencil-like shape with size of 20 cm

in length and 0.5 cm in diameter. Identification and anatomical observation of wood

samples were according to IAWA. Preparation of wood fiber maceration followed the

Schultze method, then lumen diameter and fiber length were measured by microscope.

Physical properties of wood were determined to obtain specific gravity and volume

shrinkage. Absolute bending and compression strength, as well as the strength class were

estimated through the specific gravity values based on Indonesian Forestry Classification.

As much as 22 wood species have been collected, where nine of which were lesser-known

wood species. Lesser known wood species, which having potential as sawn timbers with

strength class II were Ki Ahid (Intsia sp.) and Kasai (Kingiodendron sp.); and that with

strength class III were Kabaruk (Antiaris sp.) and Ki Ono (Combretocarpus sp.). From the

fiber morphological measurements showed that the smaller the diameter of the lumen and

the longer the fiber, the specific gravity of wood increased.

Keywords : fiber length; lesser-known wood species; lumen diameter; specific gravity;

strength class

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61

Optimum Tempertature for Germanation and Seedling Characterization of

The Three Millet Accessions

N Setyowati1*

, P Lestari1 and A H Wawo

1

1Research Center for Biology, Indonesian Institute of Sciences,

Cibinong Science Center, Cibinong-Bogor 16911, Indonesia

* [email protected]

Abtract. The research has been conducted at the Physiology Laboratory of Research

Center for Biology LIPI. The study aims to provide information about the prediction of

millet plants distribution based on optimum temperature of germination. Millet

accessions of Polman, Gambirmanis, and Enggano used as research materials. The study

was arranged according to the factorial RCBD. The treatment consisted of 2 factors,

namely accession (Polman, Gambirmanis, and Enggano) and germination temperature

(10,20,30 and 40°C), with 3 replications. The seeds were germinated in Petridish. Each

accessions of millet have distinctive characters. The colour of the dehusked grain affects

the colour of flour that turned to affects the quality of millet products. The grain colours

of Accession Polman were white, Gambirmanis has gray colour, and Enggano seeds were

yellowish. Germination temperature affects seedlings growth. Millet seeds can germinate

well at temperatures of 10-30oC. The optimal germination occurs at 30

oC that indicated

by the optimal seedlings growth. At this temperature, Polman's accession had the best

performance. The seed that germinated at higher temperature (40oC) appeared to be

seized, with a low growth rate. It is assumed that millet can be adapted well in the

lowlands to highland. However, this plant grows optimally in the lowlands with a

germination temperature of 30oC.

Keywords: accession, flour, germination speed, highland, lowland, seed viability.

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Pcr Column Contamination Gave False Positive Result for Cherax

Quadricarinatus Densovirus (Cqdv) In Sf 9 Cell Cultures

Dewi Syahidah1*, Jennifer Elliman

2, and Leigh Owens

2

1 Pathology Laboratory, Institute for Mariculture Research and Fisheries Extension

(IMRAFE) Gondol, 81155 Indonesia

2 College of Public Health, Medical and Veterinary Science (CPHMVS) JCU, 4814 Australia

*Corresponding author: [email protected]

Abstract: The DNA binding columns used in many extract kits to extract and purify have

been shown in previous studies to be responsible for introducing foreign DNA such as

mouse DNA into test samples. This present study aimed to in vitro propagation C.

quadricarinatus densovirus (CqDV) in Spodoptera frugiperda cell cultures (Sf9). The

infection of CqDV was detected with a routine histology by H&E staining and with

molecular analyses by PCR. Sf9 was sub-cultured in 6 75TC flasks with Sf-900 II SFM

media and Streptomycin (5ml/l). RT-PCR was performed using primer set of

CqPV5F-CGC TGT GGA GAG TGC ACT AGA G- and 2ARCR- TCT GAA TCA ATC

TCC TCA CG-. Infected cells (80% monolayer) received virus inocula (200µl) from

prepared virus-inocula of CqDV-infected redclaw crayfish (C. quadricarinatus) at JCU

Australia, whereas 1xPBS (200µl) was added into uninfected cells (80% monolayer). Both

cell groups were passaged every 5 days when cells are fully monolayer. Fifty percent of

cells‟ suspension from each flask were collected for 12 passage times and subjected to HE

and PCR analyses. The results showed that HE stains failed to show the effect of virus

infections in infected cell groups. However, the amplification of both infected and

negative cell groups showed positive results (280bp). Therefore, we extract and test our

non-template control samples (NTC) to detect any possible contamination of our PCR

columns. Interestingly, we find out that the PCR amplification of our NTCs also positive

for 280bp. This false positive result showed contamination in our PCR columns used. A

non-column PCR method might give more accurate positive results for CqDV.

Keywords: PCR column, contamination, CqDV, Sf9

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63

Economic Valuation of Forest Fruits Utilization in Batak Toba Community

Alfonsus H Harianja1,2

, Anisse M. Sinaga3, Ferry A Hawari

4 and Ridwan Fauzi

5

1,5

Research and Development Center for Environmental Quality and Laboratory, Forestry and

Environmental Research, Development and Innovation Agency, Ministry of Environment and

Forestry of the Republic of Indonesia, PUSPIPTEK, Building 210 Serpong, South Tangerang,

Indonesia. 2 e-mail: [email protected]

3Aek Nauli Environmental and Forestry Research and Development Agency

4Orangutan Information Centre, OIC

Abstract. Batak Toba community that settled around forests utilize forest-fruit grown

around their settlement. This research aims to quantify the economic value of the

forest-fruit using Local User Value Index (LUVI) assessment as most of the fruits have

not yet traded. Research carried out by employing survey on the Sitoluama and

Simardangiang Villages in North Tapanuli Regency in 2015. Respondents (n=64) were

chosen purposively, based on gender and age classification. The results showed that

there were 28 kinds of fruits are utilized, which were categorized in to four utilization

categories, namely as a fresh fruit, processed fruit, spices and medicines. In Sitoulama

Village, five most important fruits based on LUVI are kapundung (0.50), hopong (0.41),

sihim (0.32), handis (0.32) and mobe (0.19). Meanwhile, in Simardangiang Village were

kapundung (0.56), hopong (0.52), sotul (0.48), harimonting (0.47) and habo (0.42).

Based on these findings, the fruit forests that have domestication potency are kapundung,

hopong, sihim, handis, mobe, harimonting, sotul and habo.

Keywords: forest fruits, kapundung, mobe, harimonting, LUVI

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64

Probiotic Powder Production for Cattle by Using Response Surface

Methodology

Rohmatussolihat, Roni Ridwan, Nurul Fitri Sari, Rusli Fidriyanto, Wulansih Dwi Astuti,

Yantyati Widyastuti

Research Center for Biotechnology-Indonesian Institute of Sciences

Jl. Raya Bogor KM 46 Cibinong 16911

Abstract. At present, the use of antibiotics and growth hormones for livestock has been

prohibited by the regulations. Probiotics are one of the additives that are safe and

permitted by applicable regulations, and also can replace the use of antibiotics. Probiotics

are live microorganisms used in livestock to improve microbial balance and positive

effects on host animals. The purpose of this study is to optimize the production media of

probiotic powder using Central Composite Design-Response Surface Methodology

(CCD-RSM). The RSM-CCD is prepared using four variables, namely starch, CaCO3,

Corn Gluten Meal (CGM) and zeolite, with five level combinations. The drying process

of probiotic powder uses a vacuum oven at 35-450C for 20-30 hours, the vacuum process

is carried out using a vacuum pump for 1-3 hours. The probiotic survival amount is

measured by the total plate calculation method. Statistical analysis showed that the

viability of probiotics was significantly affected by CaCO3, CGM and zeolite. The

optimal starch concentration was 29.9%, CaCO3 44.42%, CGM 29.96%, zeolite 55.58%

with a maximum estimate of probiotic results surviving at 98.89%, which significantly

increased by 50.22% . These results indicate that the percentage of probiotic survival

increased by 40.97% when the optimal concentration of ingredients was used.

Key words: probiotic, production, cattle, response surface methodology

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65

The Effects of Clove Oil On Coral, Trachyphyllia Geoffroy, Using

Biomarkers; Color Change of Coral, Density And Mitotic Index of

Zooxanthellae

Sri Wahyuni Rahim; Khusnul Yaqin; Hadiratul Kudsiah

Department of Fisheries, Faculty of Marine Science and Fisheries, Hasanuddin University,

Makassar, Indonesia

Correspondent email: [email protected]

Abstract. The use of clove oil as an alternative to environmentally friendly cyanide is one

form of sustainable use of coral reef ecosystem resources. However, it is necessary to

consider the quality of the post-capture coral before the application of clove oil. This

research aims to analyze the quality of Trachyphyllia geoffroy coral after clove oil

exposure. The experimental design used a factorial design, consisting of 7 variations of

clove oil concentration and 5 time variations with 3 repetitions. The coral exposed clove

oil with different concentrations (control, 20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm, 70

ppm) for 5 minutes. Subsequently transferred to the aquarium recovered for 60 seconds

then transferred to a tank for analysis of changes in coral for 17 days. The results showed

that the exposure of clove oil with a concentration of 20 - 70 ppm in corals did not cause

coral discoloration, there was no significant difference between the density and mitotic

index of the coral and variations in clove oil concentration after exposure (p> 0.05). This

shows that clove oil with a concentration of 20-70 ppm has not caused changes in color,

density and mitotic index of zooxanthellae in Trachyphyllia geoffroy corals.

Keywords: clove oil; mitotic index, Trachyphyllia geoffroy; zooxanthellae

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66

Identification and Test of Fungi On Kaliandra (Calliandra calothyrsus) Seed

Which Is Potential As Antagonist Agent

Tati Suharti1*, Wildani Hanifah

1, Sri Listiyowati

2, YMM Anita Nugraheni

1, Danu

1

1Forest Tree Seed Technology Research and Development Center (BP2TPTH), Bogor 16001,

Indonesia 2Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University Bogor

16680, Indonesia

*Corresponding author : [email protected]

Abstract. Kaliandra (Calliandra calothyrsus) is an economic important plant and used as

a renewable energy source. Seed-borne fungi can be beneficial or detrimental. One of the

beneficial roles of fungi is as an antagonistic agent. Kaliandra seed are commonly

infected by pathogenic fungi such as Fusarium sp., Aspergillus sp. and Rhizopus sp. The

purpose of this research was to identify the fungi in Kaliandra seeds which is potential as

antagonistic agent and study their ability in inhibiting growth of pathogenic Fusarium sp.,

Aspergillus sp. and Rhizopus sp. The seeds obtained are from Papua. Fungi was isolated

by soaking the seeds with 1% NaOCl for 3 minutes then washed with sterile aquades, it

was repeated up to three times. Furthermore, the seeds are sown on moist paper media

and incubated until a fungus colony emerges which is expected to be a potential

antagonistic agent. The result showed that there was one isolate of fungi that was

potential as an antagonistic agent, it was Talaromyces sp. The inhibition percentages of

Talaromyce sp against Aspergillus sp., Fusarium sp. and Rhizopus sp. were 67.92%,

59.36%, 8,75%, respectively.

Keywords: antagonist agent, fungi, seed borne pathogen, Talaromyces sp.

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67

Potency of Indonesian Nutmeg Oil for Combating Halitosis

Yanti*, Ika Kristiyaningjati, and Bibiana Widiyati Lay

Faculty of Biotechnology, Atma Jaya Catholic University of

Indonesia, Jakarta 12930, Indonesia

*Corresponding author: [email protected]

Abstract. Halitosis is an oral health problem for people who has caries and periodontal

disease. About 85-90% of the causes of halitosis is bacteria that lives in the oral cavity.

Nutmeg seed (Myristica fragrans) is a spice originated from Banda Island, Moluccas

(Indonesia) that has been claimed to exert antibacterial activity against Gram-positive and

Gram-negative bacteria. In this study, we investigated whether essential oil extracted

from nutmeg seed at various doses (20-100 g/ml) had antihalitosis effect against

Streptococcus mutans by conducting biofilm, volatile sulphure compounds (VSCs), and

pH-stat assays. Essential oil from nutmeg seed was extracted using hexane and identified

for its major compounds by gas chromatography mass spectrophotometry (GC/MS).

Major compounds in nutmeg oil were myristicin, methoxyeugenol, and

dehydrodieugenol. Nutmeg oil dose dependently prevented the S. mutans biofilm

formation up to 60% and damaged the established biofilms up to 75%, indicating its

antibiofilm potency for preventing and eradicating the S. mutans biofilms. Nutmeg oil

also inhibited the VSCs production up to 71% and reduced total acids produced by S.

mutans in 20 minutes resulting in pH of 5.35 from the initial pH of 7.00. Therefore,

nutmeg oil could be considered as a potential nutraceutical for application of oral

healthcare products.

Keywords: antihalitosis activity, nutmeg oil, oral healthcare, Streptococcus mutans

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68

The Prospect of Microorganism in the Silver Nanopaticle Biosynthesis to

Enhance Antibiotic Drug Activity as an Alternative Solution to Combat

Resistances

Mega Ferdina Warsito

Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Komplek

CSC-LIPI Jl. Raya Bogor Km 46, Cibinong 16911, West Java, Indonesia.

Corresponding author: [email protected]

Abstract. Antimicrobial resistance (AMR) has become major concern worldwide.

Nanotechnology become an alternate solution to combat these emerging problems, through

the formulation of antibiotic drugs using nanoparticle as the delivery system to enhance the

activity and reduce toxicity. Interaction between nanoparticle and bacteria can cause

membrance distruption and toxicity to pathogenic microorganism, thus the combination of

silver nanoparticle and antibiotic subtances hopefully can increase therapetic efficiency.

Several microorganism, such as actinomycetes, Eschericia coli, Pseudomonas sp,

Aspergillus flavus, Bacillus sp., Penicillum and Saccharomyces cerevicae have been

known to be able to synthesis silver nanoparticle. This is an alternative method to the

chemical and physical synthesis processes, which is more environmental friendly. The

biological synthesis process also considered easy and efficient compare to the conventional

one. Furthermore, the biogenic nanoparticle have been proved to have board spectrum

activity against gram negative and gram positive bacteria, including the pathogenic fungi.

This review will discussed the microbial agent that have been known for their silver

nanoparticle synthesis ability and their usage in the delivery system of antibiotic subtances.

Keywords : silver nanoparticle, microorganism, resistance, biosynthesis

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69

Review: Fungal Glycoside Hydrolases of White-rot Fungi for Cellulosic

Biofuels Production

Sunardi1,4*

, Wiwin Tyas Istikowati2, Futhoshi Ishiguri

3, Shinso Yokota

3

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Lambung

Mangkurat University, Banjarbaru 70714, Indonesia 2Faculty of Forestry, Lambung Mangkurat University, Banjarbaru 70714 Indonesia

3Faculty of Agriculture, Utsunomiya University, Utsunomiya, Tochigi 321-8505, Japan

4Wetland-Based Materials Research Group, Lambung Mangkurat University, Banjarbaru

70714, Indonesia

*Corresponding author: [email protected]; [email protected]

Abstract. The second generation bioethanol production from lignocellulose materials

through environmentally friendly methods is one of the biggest challenges on industrial

application. Enzymatic hydrolysis of cellulose has more benefits compared with the acid

hydrolysis This method has the good specificity, low consumption of energy and

chemicals, and is more environmentally friendly. However, the utilization of lignocellulose

for bioethanol production through enzymatic methods is still confronting several

difficulties for commercialization. Cellulose hydrolysis step has been reported to be the

bottleneck of bioethanol production by enzymatic process, and the major barrier of this

process is high price of enzymes, which making the process less economically feasible. For

this reason, many developments are still needed in cellulase production from various

organisms for cellulose saccharification. White-rot fungi have received much

consideration for their valuable enzyme systems which can effective degrade

lignocellulose biomass. These fungi could secrete extracellular oxidative and hydrolytic

enzymes that degrade lignin, hemicellulose, and cellulose. This review provide a

complete overview of the glycoside hydrolases enzymes production by white-rot fungus,

such as endoglucanase, exoglucanase, beta-glucosidase, cellobiose dehydrogenase and

lytic polysaccharide monooxygenase. The use white-rot fungus for low cost glycoside

hydrolases enzymes production might be fascinating for second generation bioethanol

production.

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70

Comparison of Dissolved Organic Carbon (DOC) and Physicochemical

Variables of Peat Water Among Three Tropical Peat Swamp Forests

Siti Sundari*

Research Center for Biology, Indonesian Institute of Sciences,

Cibinong Science Center, Cibinong-Bogor 16911, Indonesia

*Corresponding author: [email protected]

Abstract. Comparison of dissolved organic carbon (DOC) and physicochemical variables

of peat water had been conducted in three tropical peat swamp forests of Central

Kalimantan. They were pristine peat swamp forest in Sebangau (SF), burnt peat swamp

forest (KB) in Kalampangan, and drained peat swamp forest (KF) in Kalampangan with

dominant tree species were Combretocarpus rotundatus and Cratoxyllum arborescens.

This research aims to investigate effects of drainage and fire on DOC concentration and

physicochemical variables of peat water in the tropical peat swamp forests. The DOC

concentration was analysed by total organic carbon (TOC) analyzer, while

physicochemical variables including electrical conductivity (EC), pH, and ions were

analysed by EC meter, pH meter, and ion analyzer, respectively. Research results showed

that the highest DOC concentration was measured in KF, whereas the lowest DOC

concentration was in KB.

Keywords : DOC; drainage; fire; peat swamp forests; physicochemical variables

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71

Agroforestry Practices In The Use of Dry Land In Gunungkidul District,

Yogyakarta, Indonesia

Sudomo A.1 dan Gerhard E. S.

2

1Research and Development Institute of Agroforestry Technology, Ciamis, West Jawa,

Indonesia 2World Agroforestry Center-ICRAF, Bogor, West Java, Indonesia

Email: [email protected]

Abstract. This research is aimed to improve the contribution of agroforestry for villagers

as the agro-ecosystem. The specific purpose is to find agroforestry practices that has been

applied by the local people to support food security on dry land utilization in Bejiharjo of

Karangmojo Sub-District of Gunungkidul District of Yogyakarta Province of Indonesia.

There were 90 plots and belong to 66 farmers/ household to be observed. Interview on land

owner has been conducted to find out the utilization of plant species and land cultivating

technique. The research showed that planting pattern on four types land utilizations (home

garden, farming land, area between farming land, and private forest) was tree mixed

garden, agrisilviculture, silvopasture, and monoculture. Types of applied agroforestry

pattern were alleycropping, border trees, and mix/ random. Types of dominant tree in local

people land is Tegtona grandis (70.75%), Swietenia mahagoni L (9,8%), Acacia mangium

(3,18%), Sesbania grandiflora (0.05%), Leucaena glauca (L) (1.78%), and Gliricidia

sepium (0.16%). The dominant types of fruit tree were Cocounut nucifera (3.18%),

Mangifera indica (1.8%), Syzygium sp (0.64%), Gnetum gnamon (1.6%), and Manilkara

kauki (0.21%). The dominant farming plants on land among farming area were corn,

peanuts, chili, and cassava. Types of trees that grow under canopy were Colocasia

esculanta, Maranta arundinacea, Dioscorea esculenta, and Canna edulis. Medicine trees

that grow under canopy were ginger, turmeric and Alpinia galanga. Animal feeds were

grass, leaves, and twigs. Agroforestry is subsistent and semi-commercial applied as a kind

of adaptation with bio-physical environment, limited land area (<0,5 ha/ household) and

food needs.

Keywords: Agroecosistem, agrosilviculture, dry land, food security, village.

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72

Irradiated Foxtail Millet (Setaria italica (L.) P.Beauv) : Agronomic And

Physiological Performance Under Low Light Intensity

Titi Juhaeti

Bidang Botani, Pusat Penelitian Biologi LIPI

Jl. Raya Jakarta Bogor km 46 Cibinong 16911 Indonesia

Corresponding author: [email protected]

Abstract. The research was carried out to study the agronomic and physiological

performance of irradiated foxtail millet (IFM) under low light intensity as an effort to

obtain shade tolerant foxtail millet. Experimental design was RCBD with two factors.

The first factors were the level of light intensity 0% and 50% shading intensity. The

second factors were IFM namely GM75.16, GM25.12, M2GM3, GM100.10, GM75.14,

GM100.20 and GM0.5 (control, without radiation). The observed variables included plant

growth, chlorophyll content (CC), photosynthesis rate (PR), and panicle production (PP).

The research showed that low light intensity decreased CC and PR value; thereby

reducing plant growth and production. The CC value in 50% shading (33.02 SPAD) was

significantly different with 0% shading (47.56 SPAD). The PR value at 50% shading

(7,112µmolm-2

s-1

) was significantly different from 0% shading (19,379µmolm-2

s-1

).

Observations on IFM showed that GM 75.16 potential as shade tolerant plant. The plant

height and the leaves number of GM 75.16 were not significantly different with control.

The GM75.16 (17.03g) showed the highest PP and significantly different with GM25.12,

M2GM3, GM100.10 and GM100.20 but not significantly different from control (16.24 g)

and GM75.14 (14.10g). It can be concluded that the decreased of CC and PR under low

light conditions resulting in decreased growth and crop production. Irradiated foxtail

millet GM75.16 was potential as shade tolerance foxtail millet.

Keywords: agronomic, foxtail millet, low-light intensity, physiological

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73

Morfological Characterization of Local Durian from Banten Province

Zuraida Yursak, Ulima D Amanda, Dewi Widiyastuti, Pepi Nur Susilawati

Peneliti Balai Pengkajian Teknologi Pertanian Banten

Jl. Raya Ciptayasa KM.01 Ciruas Serang Banten 42182

Email : [email protected]

Abstract. Durian (Durio zibethinus) is an exotic fruit with good varietal improvement

prospects. Indonesia is one of the countries with high durian diversity, one of which is

located in Banten Province. This study aims to determine the diversity and relationships

of several local durians in Pandeglang and Lebak Regencies of Banten Province based on

the morphological characters of leaves, flowers and fruit. The durian samples were

obtained by purposive sampling and interview methods. The characterization was carried

out according to the guidelines of the "International Union for the Protection of New

Varieties of Plant" including the description of stems, leaves, flowers, fruits, and seeds.

After characterization, scoring, standardization, and similarity index (IS) were calculated

between samples. Cluster analysis using Cluster Observation with Minitab software. The

analysis was conducted on 97 characters in 14 local durian accessions. Dendogram

obtained shows 4 groupings in the Similarity Index of 35.19%: Cluster 1: Si Ceer, Si

Bintang, Si Radio, Si Jangkung; Cluster 2: Kempong, Grinsing, Kadu Katung, Si Cayut;

Cluster 3: Si Bening, Hauk, Kueh Kuning; Cluster 4: Kueh Hijau.

Keywords: Durio zibethinus, relationships, morphological characters

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The Diversity Of Garlic Bulbs And Cloves Quantitative Character In Local

Garlic Collection Of East Java AIAT

Ratih Sandrakirana1*

, Maureen Chrisye Hadiatry1, and Baswarsiati

2

1,2

East Java Assessment Institute for Agricultural Technology (AIAT)

Jalan Raya Karangploso KM.4 Malang-East Java, Indonesia

*Corresponding author: [email protected]

Abstract. Since 2017, garlic has become one of the important commodities that received

special attention from government considering its imbalance conditions in the domestic

market of garlic. Based on Statistics Indonesia‟s data, domestic garlic production is only

able to meet 5% of national needs. For that purpose, the government has established a

self-sufficiency plan for garlic in 2045 through increased domestic production. However,

the limitation of clove quality and the limitation data of the character description for

garlic accessions has become a major constraint in itself in terms of the similarity of bulb

characters and cloves between accessions. AIAT has conducted an exploration of garlic

germplasms to several areas of production centers as a source of planting materials for

clove production. The objective of this study is to collect and to identify differentiated

characters from the garlic accessions. This activity took place from August 2017 to

March 2018 in Batu, Malang, Mojokerto, Pasuruan, Probolinggo and Temanggung

Regency. From the exploration activities obtained the results in the form of bulb of 4

varieties and 3 garlic accesions. Eight characters were observed, which is consist of bulb

weight character, bulb diameter, bulb length, clove weight, diameter of clove and cloves

length. In addition, there are two characters that are the ratio of the length/diameter of the

bulbs and cloves indicating the diversity between accessions and potentially to be used as

differentiator between accessions.

Keywords : Bulbs, accessions, cloves, exploration, character

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75

Detection of Adulteration Activities in Edible Bird’s Nest Using NMR

Metabolomics

Yong Chin Hong1, Syahidah Akmal Muhammad

1,2,*, Fatimatuzzahra Abdul Aziz

3, Ng Jing

Sheng1, Fatin Ilyani Nasir

2

1 School of Industrial Technology, Universiti Sains Malaysia, 11800, USM, Penang, Malaysia

2 Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 USM, Penang,

Malaysia 3

School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, USM,

Penang, Malaysia

*Corresponding author: [email protected]

Abstract. Edible bird‟s nest (EBN) is the nest of swiftlet made from the saliva of male

swiftlet (Aerodramus or Collocalia species). Due to its nutritional values, EBN is

recognized as a premium food and highly in demand among Chinese community. EBN is

commonly adulterated with cheaper ingredients and efforts are being made to combat the

activities using different analytical techniques. Metabolomics is an approach to analyse the

metabolome, both small molecule intermediates and products of metabolism. Hence, in

this study, we employed nuclear magnetic resonance (NMR) metabolomic fingerprinting

to detect adulteration activities in EBNs. A sample preparation procedure was developed

for this purpose. Briefly, 35mg of EBN sample was heated in 1 mL distilled water for 30

minutes, followed by filtration. The filtrate was then mixed with pH 7 buffered solution

containing an internal standard before analysis with NMR. Once the sample preparation

was finalized, it was used to analyse more than 30 EBN samples for their metabolomic

fingerprints which were then subsequently profiled using chemometrics. Overall, this work

showed that the discrimination of authentic EBNs from the adulterated ones using NMR

metabolomics was successful and the technique promises to be an indispensable tool for

food authenticity work in the future.

Keywords : Edible bird's nest; Nuclear Magnetic Resonance, Metabolomics,

Adulteration, Food authenticity

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76

Effect of Amphipilic Lignin Derivatives (A-LD) Surfactant Addition on the

Fermentation Process of Sorghum Bagasse Kraft Pulp for Bioethanol

Production

W Fatriasari1,*

, A Karimah2, F Falah

1, S H Anita

1

1Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI),

Jl. Raya Bogor Km. 46, Cibinong Science Center, Cibinong, Bogor 16911, Indonesia 2Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Gadjah Mada

University, Indonesia.

Email: [email protected]

Abstract. This research was intended to study the effect of Amphipilic Lignin Derivatives

(A-LD) surfactant addition on the bioethanol production by using simultaneous

saccharification and fermentation (SSF) system. A-LD synthetis was obtained from single

step isolation of lignin from black leachate as a by-product of the Acacia mangium

kraft process. This synthesis was carried out by reacting single step lignin with the

polyethylene glycol (PEG) which was oxidized at 60 ºC for 1 h. SSF in this study was

performed by using Saccharomyes cerevisiae InaCC Y93. Cellulase enzyme of 50 FPU/g,

10% and 20% yeast inoculum, and 15 mg/mL A-LD were added into 5.37 g (wet weight)

of sorghum bagasse kraft pulp. The concentration of reducing sugar and the ethanol

content were analysed every 24 h for 72 h and also at 89 h. The ethanol concentration,

ethanol yield, and the highest percentage of theoretical ethanol yield were 4.91 ± 0.10 g/L,

0.06 ± 0.001 g/g and 11.29 ± 0.22%, respectively. They were obtained by 20% of yeast

inoculum for 24 h fermentation on SSF system and addition of A-LD surfactant. The

addition of ALD on SSF process can increase the ethanol production and also shorten the

fermentation time.

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77

Traditional Beekeeping of Closed Nested Native Honeybees (Apis spp.)

From Four Islands of Indonesia

Sih Kahono1*

, Erniwati1, Djunijanti Peggie

1, & Eko Sulistyadi

1

1Research Center for Biology, Indonesian Institute of Sciences,

Cibinong Science Center, Cibinong-Bogor 16911, Indonesia

*Corresponding author: [email protected]; [email protected]

Abstract. Three species of closed nested native honeybees, Apis cerana, A. koschevnicovi,

and A. nigrocincta, are recorded in Indonesia. Except of A. cerana distributes widely, the

others are island and geographic endemics. The bees live in nature adapt and synchronized

to their local environmental conditions. Local people usually well evolve to conduct

traditional beekeeping, however a few of them have not been informed or published. We

conducted research on traditional beekeeping of four locations of Tasikmalaya (West

Java), Kayan Selatan (North Kalimantan), Peleng (Banggai Kepulauan) and Bawean (East

Java) islands. General activity of the traditional beekeeping was beginning by collection of

both honey of colonies from nature, then transferred the colonies into unframed wooden

boxes or holed dry trunks, and finally it settled at safety places close to the house. After

honey was harvested, a few of them keep stay in the boxes, however most of them escape to

the nature. This traditional beekeeping system follows natural phenology that have been

continued for long times.

Keywords: traditional beekeeping, Apis cerana, A. nigrocincta, A. koschevnicovi,

Indonesia

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78

Bioplastic from Modified Cassava Starch, Poly Vinyl Alcohol and Citric

Acid

Firda Aulya Syamani1*, Wida Banar Kusumaningrum, Fazhar Akbar and Bernadeta Ayu

Widyaningrum2

1Research Center for Biomaterials, Indonesian Institute of Sciences,

Cibinong Science Center, Cibinong-Bogor 16911, Indonesia

*Corresponding author: [email protected]

Abstract. Food packaging plastic from petroleum-based sources can be a problem to the

environment due to its non-biodegradability property. The development of bioplastic from

modified cassava starch will be an alternative to overcome that problem. Since

starch-based bioplastic, usually has low mechanical strength and damage if exposed to

water, it is necessary to modified with poly vinyl alcohol (PVA) and citric acid. Bioplastics

are made by heating a solution of modified cassava starch (cassava starch: distilled water =

1:20) at 75 °C for 1 hour on hot magnetic stirring plate. After starch solution was

gelatinized, glycerol (5% based on starch weight) was added, then PVA solution (1, 3, 5%

based on distilled water weight) was inserted gradually. The stirring was continued for 30

min. Afterward, citric acid (5% based on starch weight) was added into the solution and

stirring was continued for 15 min. The bioplastic solution is then poured into a (20x20) cm

acrylic sheet with a thickness of ± 5mm. Furthermore, it is cooled at room temperature for

3 days so that the bioplastic sheet is ready for mechanical strength testing based on ASTM

D 882-75b Tensile Properties of Thin Plastic Sheeting, and for thermal properties testing

with Thermo Gravimetry Analyzer.

Keywords : bioplastic; modified cassava starch; tensile properties; thermal properties

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79

Mechanical Properties Investigation of Green Composite from Ramie

(Boehmeria nivea (L.) Gaud) and Epoxy

Ismadi1, Safira Vitasasti

2, Sukma Surya Kusumah

1

1 Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI), Bogor, Indonesia

2Match and Sciences Faculty of Padjadjaran University, Sumedang, Indonesia

Email : [email protected]

Abstract. In the last decade, requirement of environmentally friendly materials increased

significantly. Green composite materials were developed to provide the materials

requirement. One of green composite materials has been developed is natural fibre

reinforced epoxy composite. Ramie (Boehmeria nivea (L.) Gaud) fibre and bio-epoxy

were potential to arrange stronger and friendly composite. The paper present the

evaluation of rami fibre-epoxy composite in mechanical and physical characterization.

Rami fibre was arranged in one and three layers. The concentration of ramie fibre was

varied in 5, 7.5 and 10% of w/w composite weight. Hardener of epoxy matrix was

variated in 50 and 25% of w/w matrix weight. In composition of 50% hardener (w/w of

matrices), highest of Tensile Strength is 10,7 MPa. In 7,5% rami fibre content. Maximum

flexural strength was 34,5 MPa in 10% of rami fibre content. In the 25% of hardener

(w/w of matrices), the maximum tensile and flexural strength are 16,7 MPa and 33,57

MPa for 5% rami fibre content (w/w composites weight). In the three layers of fibre

mode, maximum strength of composite was 42,5 MPa for 10% of rami fibre content (w/w

of composite weight), and maximum flexural strength was 34,95 MPa for 7,5 % of rami

fibre content. In 25% hardener content, maximum of tensile strength was 45,8 MPa for

5% rami fibre content (w/w of composite weight), and maximum flexural strength was

31,35 for 10% rami fibre content. The lowest of water absorption of ramie-epoxy

composite was 6,75% for 7,5% of ramie fibre content in single layer mode. In the three

layer mode, the lowest water absorption was 6,72 % for 5% of rami fibre content.

Keywords: Green composite, tensile strength, flexural strength, ramie fiber, epoxy

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80

Bioconversion of artemisinin by endophytic fungi associated Artemisia sp.

grown in Indonesia and Japan

Shoji Maehara1*, Ahmad Fathoni

2, Muhammad Ilyas

2, and Andria Agusta

2

1Faculty of pharmacy and pharmaceutical sciences,

Fukuyama University, 985, Sanzo, Higashimura-cho,

Fukuyama, Hiroshima, 729-0292, Japan 2Research Center for Biology, Indonesian Institute of Sciences,

Cibinong Science Center, Cibinong-Bogor 16911, Indonesia

*Corresponding author: [email protected]

Abstract. Artemisinin is a well-known anti-malarial drug that can be found in leaf of

Artemisia plant. The plant of Artemisia is growth widely in Europe, North America, North

Africa and Asia. Therefore, Artemisia plant that is called Wormwood or Mugword in

English, consist many plant species. The Indonesian mugwort or “baru cina”, and Japanese

mugwort or “Yomogi” has long been used as culinary herbs and medicinal plant. In this

report, we deal with symbiotic microorganisms in Artemisia plant grown in Indonesia and

Japan. Totally 15 endophytic fungi were isolated form Artemisia from Indonesia, 21

endophytic fungi from Artemisia from Japan. It was found that the composition of each

endophytic fungi varies depending on the growth environment (region). Isolated

endophytic fungi that promote the bioconversion reaction using artemisinin as a substrate

have been clarified by screening tests. Interestingly, it was found that the microbial

conversion reaction of each endophyte was different depending on the growth environment

(region). Here we show reactions by endophytic fungus DnAvCw-1 and Y13-2 associated

Artemisia plant grown in Indonesia and Japan. It was revealed that artemisinin was

deoxygenated by DnAvCw-1 and hydroxylated by Y13-2.

Keywords : artemisinin; endophytic fungi; bioconversion; anti-malaria; Artemisia

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81

Larvicidal Activity of Cerbera odollam against Culex quinquefasciatus

(Diptera: Culicidae)

Didi Tarmadi1*

, Sulaeman Yusuf1, S. Khoirul Himmi

1, Maya Ismayati

1, Ikhsan Guswenrivo

1,

Titik Kartika, Dita Meisyara1

1Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor

KM. 46, Cibinong, Bogor 16911, Indonesia

*Corresponding author: [email protected]

Abstract. Mosquito is a major public health problem as vector of hazard human disease.

Culex quinquefasciatus, a vector of filariasis is widely distributed in tropical and

subtropical zones. In fact, filariasis is primarily an urban disease of the tropics and

hazardous disease. Plants can be used as an alternative source of mosquito larval control

materials to substitute chemical materials. This study aimed to examine larvicidal activity

of Cerbera odollam against a filariasis vector, C. quinquefasciatus. We applied

twenty-five 2nd instar of C. quinquefasciatus into the crude extract solutions derived

from rind, steam bark, leaf and seed kernel of C. odollam. In addition, the present study

also examined the larvicial activity from fractionated extracts derived from seed kernel of

C. odollam. The results showed that larvicidal activity was very low for crude extracts

from, rind, steam bark, and leaf, however, the present study detected moderate larvicidal

activity for seed kernel. The larvicidal activity of fractionated extract showed high larval

mortality in ethyl acetate, and moderate in n-hexane.

Keywords: larvicidal activity, Culex quinquefasciatus, natural extractive, filariasis

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82

Conservation of Kelulut (stingless bees) in East and North Borneo,

Indonesia

Syafrizal1*, Irawan Wijaya Kusuma

2, Oshlifin Rucmana Saud

2, Restina Wiandany

2, and

Harmonis2

1 Faculty of Mathematic and Science, Mulawarman University, Samarinda, Indonesia

2 Faculty of Forestry, Mulawarman University, Samarinda, Indonesia

*Corresponding author: [email protected]

Abstract. Southeast Asia is home to at least 9 species of native honey bees, one of which is

Kelulut (stingless bees). The Kelulut is very valuable because it is a major pollinator for

tropical plants, provides significant income for some members of the local community, and

is an important component in the food network system. In addition, the Southeast Asian

Dipterocarp forests, particularly East and North Kalimantan, appear to be adapted to

pollination by honey bees. Current concerns about the decline of plant and pollinator

species that are directly related to ecosystem functions have focused on local and global

threats to bee diversity. Because evidence of bee decline is now accumulating on broad

taxonomic and geographical scales, we review the role of Kelulut cultivation based on

species and habitat approaches. Species diversity, population size, and growth rates are

influenced by temporal-spatial variations, all of which play an important role in their

vulnerability to extinction. As such, this will enhance our understanding of the factors and

processes that are behind bee decline, to ensure the long-term survival of the bees and the

important pollination services they provide.

Keywords : bee; borneo; cultivation; diversity; pollination

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83

Allelopathy of Invasive Species Air Potato (Dioscorea bulbifera L.) and Its

Effect on Germination of Meranti (Shorea selanica (Lam.) Blume

Ade Ayu Oksari1,♥

, Irvan Fadli Wanda2,4

, Gladys Ayu Paramita Kusumah Wardhani3

Biology Department, Nusa Bangsa University, Jl. KH. Sholeh Iskandar Km 4, Bogor,

Indonesia. 161661*

Research Center for Plant Conservation and Botanic Gardens-Indonesian Institute of Sciences

(LIPI). Jl. Ir. H. Juanda No 13 Bogor, Jawa Barat, Indonesia2

Chemistry Department, Nusa Bangsa University, Jl. KH. Sholeh Iskandar Km 4, Bogor,

Indonesia. 161663

Biology Department, Faculty of Mathematics and Natural Sciences, IPB University, Bogor

16680420 09

20

*Corresponding author: [email protected].

18 (Date of abstract/manuscript submission). Revision accepted: .................... 2018.

Abstract. Dioscorea bulbifera L (Dioscoreaceae) is one of the most unutilized Invasive

plants.This Plant contains alelopathy that affects the physiological process of native

species. The study aims to know effect of allelopathy and type of Allelochemical of the

various concentrations leaf and tuber extracts of D. Bulbifera on seed germination of

Meranti (S. selanica (Lam.) Blume). The method used a Completely Randomized Design

with ten treatments and three replications. The treatment given the concentration of

extract D. bulbifera 25, 50, 75, 100%, and control. Based on the results of phytochemical

screening tests all secondary metabolites in positive leaf extract results. Saponin, tannin,

flavonoid, and steroid / terpenoid compounds are strongly detected, while alkaloid

compounds are less strong. In the tuber extract, compound compounds saponins,

flavonoids and steroids / terpenoids are detected strongly, while tannins are detected less

strongly. Alkaloid compounds are not contained in the tuber extract. The total phenolic in

leaf extract is 2.19 ± 0.13, the value is greater than the tuber extract of D.bulbifera which

is 0.96 ± 0.01. Phenolic compounds in leaf extracts consist of two types (phenol and

1,2-benzenediol). Tuber extract has 4 types of phenolic compounds. Two compounds of

the same type as phenolic compounds in leaf extracts (phenol and 1,2-benzendiol) while

the other compounds are hydroquinone (1,4-benzendiol) and 2-methoxyphenol. The

results showed that allelopathic substances and concentrations of D. bulbifera affected

the germination of S. selanica seeds. Based on DMRT test at 5% level, it was known that

allelopathy concentration of D. bulbifera significantly affected the germination capacity,

Coefficient Rate of Germination and Coefficient of Simultaneity of Germination with all

concentrations. The concentration and source of extracts that reduced the lowest seed

viability was at a concentration of 75% and 100 % with germination capacity 10.00% ±

6.32 in leaf extract and 0.00% ± 6.32 in tuber extract.

Keywords: allelopathy, D. bulbifera L., germination, S. selanica (Lam.) Blume.

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84

Morphological Characteristic of Apple Velvet (Dispyros blancoi) at

Germplasms Garden of LIPI Cibinong Collection

Puspita Deswina1*

, Novia Delliasari Aliyya Az Zahra2, Etty Hesthiati

2

1Research Center for Biotechnology, LIPI

2Agrotechnology Study Program, Agrotechnology Specialization Program, Faculty of

Agriculture, Universitas Nasional, Jakarta

*Contact: [email protected]

Abstract. Apple velvet (Diospyros blancoi) is one of the local fruits which is classified as

a rare plant with high economic value. Apple velvet has various morphologies that

require selection of various characters in order to identify the characters that are superior

and have high potential to be developed. Analysis of diversity of a population can be

identified to determine the morphological character of the plants. The notable

characteristics may include tree trunks, leaves, flowers, fruits and seeds. A study using the

quantitative and qualitative methods conducted in Germplasms Collection Garden of LIPI

Cibinong have shown that the apple velvet plants has the average height between 3.5-15

m, with stem, diameter between 14.1-72.29 cm. It has no distinctive shape of leaves such

as oval and wide oval. It has white flower crown, two shapes of fruits such as round and

oval pattern, and two shapes of seeds such as elliptic and biconvex.

Keywords: apple velvet plants, characterization, germplasm garden, rare plant

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85

Bee Pollen from Stingless Bee (Heterotrigona itama) as anti Acne*

Enos Tangke Arung1, 4**

, Irawan Wijaya Kusuma1, 4

, Ayu Mitha Sari 1, Yadi

2,4 , Sukemi

3,

Nataniel Tandirogang2,4

1. Lab. Forest Product Chemistry, Faculty of Forestry, Mulawarman University, Samarinda

2. Faculty of Medicine, Mulawarman University, Samarinda

3. Faculty of Education, Mulawarman University, Samarinda,

4. Research Center for Medicine and Cosmetics from Tropical Rainforest Resources,

Mulawarman University, Samarinda,

* This research funded by

Kemenristekdikti (No. 198/UN17.41/KL/2019)

**email: [email protected]

Abstract. In effort to search from natural resources for medicinal and cosmetic purposes.

Natural resources from tropical rain forests, especially non-timber forest products,

recently get attention such as honey, propolis and bee pollen. The stingless bee,

Heterotrigona itama produced honey, propolis and bee pollen. In this study we focused

on bee pollen ethanol extract for inhibition of Propionibacterium acnes. The diffusion

method was used and the results showed the ethanol extract of bee pollen inhibit the

growth of P. acnes. Based on these results, this extracts have the potential inhibition and

potential to be developed as a source of raw material for anti acne but certainly needs

some furthers experiment.

Keywords : Stinglees bee, bee polllen, Heterotrigona itama, Propionibacterium acnes

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86

"How Fisheries Technology Affect Production?" (Case Study: North

Gorontalo Regency)

Tikkyrino Kuriawan1 and Hakim Miftahul Huda

1

1 Research Centre for Marine and Fisheries Socio-Economics, North Jakarta, Indonesia

Abstract. Prohibition of several fishing gears has been started from 2015 by issuing

MMAF Ministry regulation no. 2 of 2015. The regulation prohibits several fishing gears;

one of them is seine nets. MMAF Ministry regulation no. 71 of 2016 details the size of

seine nets, including purse seine, which is not allowed. Fishers still believe that fishing

using purse seine with a large fleet is more profitable compared to hand line fishing gear

with a small fleet. The purpose of this study is to see whether the handline fishing gear is

a more profitable handline compared to purse seine. This study takes STBLK data from

PPN Gentuma and PPN Kwandang in the North Gorontalo Regency. The results show

that handline fishing gear is more profitable even with small boat sizes compared to large

vessels with purse seine fishing gear.

Keywords: Fishing Gear, Handline, Purse Seine, Ministry Regulation, MMAF

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87

The Effect of Sucrose Concentrations and Shoot Inoculum Density on The

Growth of In Vitro Shoot of Teak (Tectona Grandis l.) **

Witjaksono*, Indira Riastiwi, Katarina Utami Nugraheni and Gut Windarsih1

Plant Cell Tissue Culture Laboratory, Botany Devision, Research Center for Biology,

Indonesian Institute of Sciences, Jl. Raya Bogor Jakarta Km 46, Cibinong, Bogor 16911 1Universitas Islam Negeri Sultan Maulana Hasanuddin,

Jl. Syech Nawawi Al Bantani Andamui, Curug, Serang 42116, Banten

*Corresponding author: [email protected]; [email protected]

**All authors contributed equally to this work

Abstract. The teak belongs to the highly prized timber tree. Quality teak trees have been

guaranteed by planting clonal planting materials propagated vegetatively, for example by

shoot tip cutting and tissue culture. Tissue culture propagation of teak has been

commercially viable, eventhough efficiency of production can still be improved. This

research aims to study the effect of increasing shoot inoculum density and sucrose

concentration requirement for optimum growth of teak shoot in vitro. Inocula of a single

nodal shoot in the number of 5 or 9 were inoculated on modified MS basal media with

sucrose concentrations treatment of 20, 30, 40, 50 and 60 g l-1. The results showed that

standard sucrose concentration of 30 g l-1 was optimum for the growth five inocula,

while for nine inocula, the optimum sucrose concentration was 40 g l-1. Eventhough the

average shoot growth was lower for nine inocula than that of five inocula, the total shoot

growth was higher for the former. Increasing number of inocula can be used as a method

to increase the efficiency of shoot proliferation.

Keywords: teak, micropropagation, in vitro, sucrose, inoculum density, shoot culture

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Compression Strength of 9 Years-old Platinum Teak Wood

Mohamad Gopar1, Dimas Triwibowo

1, Fazhar Akbar

1, Wahyu Dwianto

1*, Akihisa Kitamori

2

and Takuro Mori3

1Research Center for Biomaterials, Indonesian Institute of Sciences,

Cibinong Science Center, Cibinong-Bogor 16911, Indonesia 2Research Institute for Sustainable Humanosphere, Kyoto University

Gokasho, Uji, Kyoto 611-0011, Japan 3Graduate School of Engineering, Hiroshima University

Kagamiyama 1-4-1, Hiroshima 739-8527, Japan

*Corresponding author: [email protected]

Abstract. Previous results on bending strength tests indicated that the 9 years-old Platinum

Teak wood could be classified into Strength Class II according to Indonesian Forestry

Vademecum Classification. Therefore, further mechanical strength tests are necessary to

recommend the wood for timber frame structures. Small clear specimens with a size of 40

mm in length x 20 mm in width x 20 mm in thickness were taken from the bottom, middle

and upper parts of 10 m stem in length of 9 years-old trees. Compression strength parallel

to the grain was tested according to British Standard BS 373-1957. The results showed that

the average oven-dried density of the bottom, middle and upper parts of stem were 0.61,

0.59, and 0.57 g/cm3, respectively. Compression strength values were slightly decreased

from bark to the pitch of wood. However, the average of compression strength values of the

bottom, middle and upper parts of stem were almost unchanged, i.e. 79.99, 75.85, and

75.71 N/mm2, respectively. According to the Classification, the wood could be classified

into Strength Class I, where the compression strength values were over 650 kg/cm2. These

results informed that the wood could be a quite suitable material for timber frame structures

of wooden construction.

Keywords : compression strength; Platinum Teak wood; timber frame structures

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89

Anti-tubercular Activity of Natural Product-derived Compounds (+)-1,1’

Bislunatin and (+)-2,2’-Epicytoskyrin A.

Listiana Oktavia1*, Vagolu Siva Krishna

2, Estharla Madhu Rekha

2, Ahmad Fathoni

1, Nikhila

Meda2, Andria Agusta

1, Dharmarajan Sriram

2

1Research Center for Biology, Indonesian Institute of Sciences,

Cibinong Science Center, Cibinong-Bogor 16911, Indonesia 2Department of Pharmacy, Birla Institute of Technology

and Science-Pilani, Hyderabad Campus, Jawahar Nagar,

Hyderabad 500078, India

*Corresponding author: gmail.com /[email protected]

Abstract. Tuberculosis (Tb) still remains a great health concern as it was estimated in

2016, 1.7 million deaths because of this disease. Current drug development pipeline is still

insufficient to completely eradicate the causative bacteria from Tb patients as recently it

was found that the occurrence of multi drug-resistant Mycobacterium tuberculosis (Mtb).

Therefore, there remains an essential need to discover and develop a novel and effective

anti-tubercular drug. In this study, two secondary metabolites isolated from endophytic

fungi Diaporthe sp. GNBP-10 derived from a gambier plant Uncaria gambier Roxb.

(+)-1,1‟-Bislunatin (Bis) and (+)-2,2‟-Epicytoskyrin A (Epi) were evaluated as

anti-tubercular agent by determination of Minimum Inhibitory Concentration (MIC)

against M. tuberculosis H37Rv. The in vitro assays for Mtb in the dormant and biofilm

mode were also conducted. The Cytotoxicity was evaluated by MTT assay in raw 264.7

macrophages normal cell. Furthermore, the ability of eradicate Mtb inside the host cell

(macrophages) was also investigated. For further evaluation, the docking study was

conducted as a preliminary study to understand the interaction between tested drugs and

potential protein target. Epi and Bis show a promising anti-tubercular activity as shown

by MIC value 0.422 µM and 0.844 µM respectively. Moreover, Epi and Bis also was

observed for having the ability to combat dormant and biofilm of Mtb model with relatively

moderate bacterial reduction in both model with log reduction between 1-2 fold. Epi shows

4% cell inhibition at the lowest tested concentration (0.01µM), while Bis remains toxic in

all tested concentrations (70%). Both compounds can penetrate through macrophages and

active to reduce the number of Mtb in 2 fold log reduction. The docking result shows that

both compound has good affinity with pantothenate kinase (PaNK) enzyme with glide

score -8.427 kcal/mol and -7.481 kcal/mol for Epi and Bis respectively.

Keywords : anti-tubercular, molecular docking, multi drug resistance, natural product, ,

pantothenate kinase

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Growth and Development of Nine Accessions of Mutant Grass Plants

(Pennisetum purpureum cv. Taiwan) in the Field of Induction Mutation of

Gamma Ray

Husni, A*1

., Maftuha2, A.A. Fatmawaty

2, N. Hermita

2, and M. Kosmiatin

1 1Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and

Development (ICABIOGRAD), Bogor, 1611 Indonesia 2 Sultan Ageng Tirtayasa University, Serang-Banten, 42124 Indonesia

[email protected]

Abstract. The purpose of this research was to know putatife mutant accession Elephant

Grass cv. Taiwan is the best in growth and development as well as the plant on the 3rd

vegetative in the field. The design used in this research is completely randomized design

one factor of 9 the treatments putative mutan accession (76, 77, 78, 118, 120, 121, 125,

129, 130) and control (139). Research result showed that the treatment in the timing

of shoots appearing at accession 77 and 125 (3.753 and 3.742 day after planting), the

largest diameter of the stem at accession 139 (2.17 cm) while the smallest trunk diameter

is found in all putative mutant accessions, the highest plants at accessions 78 and 121

(112.45 cm and 100.53 cm), the highest number of tillers at accessions 76, 77, 78, 120,

121, 129, and 130 (25.16; 18.82; 24.67; 18.67; 24.83; 25.36; and 24.51), the highest

number of leaves/plants at accessions 139 (13.43 strands), the highest number of

leaves/clumps at accessions 121 (226.70 strands), the highest number of noods/plants at

accession 139 (8.69 noods), widest flag leaf at 139 (135.82 cm2), The fastest time

appears flower (66.5-78.75 day after planting) and the highest number of flowers was

found in all putative mutant accessions (1.05-2.03 flowers).

Keywords : Accessions, development, growth, mutation, mutant grass

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Rheological and Thermal Behavior of Polypropylene–Kenaf Pulp

Composite at Different Fiber and Compatibilizing Agent Loading

Nanang Masruchin1*, Wida Banar Kusumaningrum

1, Sudarmanto

1, Sasa Sofyan Munawar

1,

Ismadi1, Putri Amanda

1, Resti Marlina

1, Lisman Suryanegara

1, Subyakto

1, Bambang

Subiyanto1 and Arif Nuryawan

2

1Research Center for Biomaterials, Indonesian Institute of Sciences,

Cibinong Science Center, Cibinong-Bogor 16911, Indonesia 2Department of Forest Products Technology, Faculty of Forestry, Universitas Sumatera Utara,

Jl. Tri Dharma Ujung No.1 Kampus USU Medan, North Sumatera Indonesia 20155

*Corresponding author: [email protected]

Abstract. Our previous study reported that Kenaf pulp fiber have higher thermal stability

compared to that of Coconut and Pineapple pulp fiber. Therefore, in this study, we

proposed the manufacturing of polypropylene (PP) natural fiber composite using Kenaf

pulp fiber as reinforcing agents. Good fiber dispersion and better thermal stability are

necessary needed on the development of green polymer matrix composite. The rheological

of polymer composite compound was studied by the torque obtained during the mixing

process between melting polymer and Kenaf pulp fiber. Further, thermal degradation of

polymer composite at different Kenaf and compatibilizing agent (maleic anhydride

polypropylene, MAPP) loading were observed using thermogravimetry analysis (TGA).

Result showed that as Kenaf pulp increased the torque slightly increased. On the other

hand, the MAPP addition decreased the obtained torque for all fiber loading. Those

indicated the polymer chain mobility improved resulted in better Kenaf fiber dispersion.

After polymer and Kenaf pulp fiber was mixed and pelletized, those pellet was grinded and

analyzed using TGA. The energy activation and thermal stability of polymer composites

are undergoing investigation

Keywords : energy activation, green composite, Kenaf, polypropylene, TGA

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Concoctions Developed from Correlations of Meliponicultured Honey with

Ethnoentomology in Langkawi, Malaysia

Abu Hassan Jalil1*, Mohd Razif Mamat

2, Azira Muhamad

2 and Nor Azfa Johari

2

1Akademi Kelulut Malaysia Sdn Bhd, 2625, Persiaran Rahmat, Teras Jernang, Bangi 43650,

Selangor, Malaysia 2Malaysia Genome Institute Jalan Bangi, Kajang 43000, Selangor, Malaysia

*Corresponding author: [email protected]

Abstract. The villagers of a 7-acre Meliponary in Northeast Langkawi, implemented

with an integrated farming system, have been using Honey mixed with popular herbs in

their traditional practices. Some of the local herbs include sea-cucumbers (gamat),

„Pepper Elder‟ (Peperomia pellucida), garlic, ginger, lemon and a mixture of honey with

'Tongkat Ali' (Eurycoma longifolia Jack). Some of the traditional practices that use Apis

honey by the villagers are replicated with stingless bee honey. Indigenous knowledge

traditionally uses Tongkat Ali's sweet honey (finely ground Eurycoma longifolia Jack and

macerated in honey) for the goal of manhood (erectile dysfunction ailments). Garlic

honey where garlic is burned and soaked in honey for two weeks, is good for prolonged

fever problems. The aim is profiling the metabolites in each of the concoctions/macerated

mixture of honey with four different local herbs. This profile will include the sugar,

amino acid and organic acid contents identified through metabolomics approach using

Nuclear Magnetic Resonance (NMR) spectrometer and Gas Chromatography Mass

Spectrometer (GCMS). From this data we will analyse and distinguish the active

compounds that are beneficial in the macerated mixture. We will ascertain the antioxidant

properties of the compounds identified. Cytotoxicity study using mammalian cell culture

will also be carried out. The resultant data will be expected to support the indigenous

knowledge in traditional treatment by providing scientific evidence to these practices.

Keywords: antioxidant properties; beekeeping; Cytotoxicity; Eurycoma longifolia Jack;

Garlic; Peperomia pellucida; sea-cucumbers; stingless bee honey

Page 107: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

93

Carbon Dioxide Absorption and Physiological Characteristics of Selected Tropical

Lowland Tree Species for Revegetation

La Ode Muhammad Muchdar Davis1, Nuril Hidayati

1*

1Research Center for Biology - Indonesian Institute of Sciences

Cibinong Science Center, Jl. Jakarta-Bogor Km 46 Cibinong 16911

*Corresponding author: [email protected]

Abstract

Biological diversity can make a significant contribution to reducing greenhouse gases in

the atmosphere. Trees form an essential part in the functioning of the terrestrial biosphere,

especially in the carbon cycle. Yet tree photosynthesis is far less studied than crop

photosynthesis. This research aims to assess the contribution of biological diversity in the

absorption of carbon dioxide (CO2) from the atmosphere by analyzing the physiological

characteristics that are related to CO2 absorption such as photosynthesis, transpiration,

stomatal conductance, leaf chlorophyll content of tree species for revegetation. The study was

conducted on selected tropical lowland trees curated at Cibinong Science Center-Botanical

Garden (CSC-BG), Indonesia. CO2 absorption, stomatal conductance, and transpiration were

measured by using an infrared gas analyzer (IRGA) photosynthesis apparatus, while

chlorophyll content was estimated using a SPAD chlorophyll-meter. Variation of CO2

absorption rate ranged from 3,42 to 20 µmolm-2

s-1

, with the highest rate was observed in

Teijsmaniadendron bogoriense, followed by Tectona grandis (19,67 µmol m-2

s-1

). Rate of

transpiration ranged from 4,7 µmol m-2

s-1

to 7,82 µmol m-2

s-1

, with that of Diospyros

blancoi being the highest, followed by Tectona grandis (7,65 µmol m-2

s-1

). CO2 absorption

and rate of transpiration are positively correlated to stomatal conductance and leaf chlorophyll

content. The stomatal conductance ranged from 0,19 µmol m-2

s-1

to 0,54 µmol m-2

s-1

,

with that of Tectona grandis being the highest, followed by Erythrina crista-galli (0,53 µmol

m-2

s-1

). Whereas the variation in leaf chlorophyll content ranged from 31 up to 78,43 SPAD.

Key Words: Biological diversity, tropical lowland trees, CO2, absorption.

Page 108: MANUSCRIPT PREPARATION GUIDELINES - LIPI

The 7th JASTIP (Japan-ASEAN Science, Technology and Innovation Platform) Symposium

Biodiversity, Genetic Resources And Innovative Bioresource Technology:

Toward 2025 Sustainable use of Biodiversity

94

Important Date:

Full Paper Submission Deadline : October 25th, 2019

Registration and Publication of Symposium Papers:

The official language of the symposium is in English. Full-paper should be written in English. Please

send your full-paper to [email protected]

Page 109: MANUSCRIPT PREPARATION GUIDELINES - LIPI