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Making linkage analysis simple
Making linkage analysis simple
(Do Ch. 5 problems!)
Bakers’ yeast
A single-celled eukaryote
Haploid and diploid
Fig. 5.14
Haploid and diploid
Fig. 5.14
Clonal (mitotic) growth
Haploid and diploid
Fig. 5.14
Haploid and diploid
Fig. 5.14
Why yeast?
Tetrad analysis
Fig 5.15
Tetrad analysis
Fig 5.15
Tetrad analysis
Fig 5.15
Cannot make histidine
Cannot make tryptophan
Tetrad analysis
Fig 5.15
Tetrad analysis
Fig 5.15
Tetrad analysis
Fig 5.15
Tetrad analysis
Fig 5.15
Tetrad analysis
Fig 5.15
Tetrad analysis
NPD = 4 recombinant progeny
Fig 5.15
Tetrad analysis
Fig 5.15
Tetrad analysis
Fig 5.15 T = 2 recomb, 2 non-recomb
Tetrad analysis example
Fig 5.15
Tetrad analysis example
“Number of recombinants = NPD•4 + T•2”
Fig 5.15
Tetrad analysis example
“Number of recombinants = NPD•4 + T•2”
“RF = NPD•4 + T•2 ”
4•Total # tetrads
Fig 5.15
Tetrad analysis example
“Number of recombinants = NPD•4 + T•2”
“RF = NPD•4 + T•2 ”
Total # tetrads
4•Total # tetrads
= NPD + 1/2T ”
Fig 5.15
Tetrad analysis example 2
Fig. 5.16
Tetrad analysis example 2
If PD >> NPD, genes are linked.
Fig. 5.16
Tetrad analysis
Fig 5.15
If linked, second law does not hold;<< 50% recombinants
Tetrad analysis
What if two crossovers?
Fig 5.15
Accurate genetic distances?
“Number of recombinants = NPD•4 + T•2”
“RF = NPD•4 + T•2 ”
Total # tetrads
4•Total # tetrads
= NPD + 1/2T ”
Where’s PD?
The real story: mapping functions
Fig. 5.17
Do Ch. 5, problem 35…
Bread mold
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
Bread mold
Fig. 5.14
Bread mold
Fig. 5.14
Each pair is identical
Mapping centromeric distance
Fig. 5.21
Mapping centromeric distance
Fig. 5.21
Mapping centromeric distance
Fig. 5.21 Number of meioses with second-division patterns = number of crossovers between locus and centromere
