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Media Release Tobacco plants affected by the virus and resistant to it. (Carl Davies CSIRO) “Pinpointing this gene represents a major step forward in understanding exactly how some viruses cause disease symptoms in susceptible organisms” Dr Ming-Bo Wange, CSIRO Major breakthrough on how viruses infect plants Reference: 11/57 CSIRO plant scientists have shed light on a problem that has puzzled researchers since the first virus was discovered in 1892 – how exactly do they cause disease? 14 July 2011 In a major breakthrough that helps us better understand how viruses cause diseases in plants – and potentially in animals and humans – Dr Ming-Bo Wang and Neil Smith of CSIRO Plant Industry have revealed a genetic mechanism that enables viral organisms to infect hosts and cause diseases. “Cucumber Mosaic Virus (CMV) is a common, destructive virus that affects a wide range of food crops and ornamental plants,” Dr Wang said. “What we found was that CMV, accompanied by a special type of viral particle called a ‘satellite’, causes its distinctive yellowing symptoms in plants by slicing a gene that makes chlorophyll, the green pigment in leaves. By preventing the production of chlorophyll, the virus causes the leaves to become partially or entirely yellowed which dramatically affects growth and productivity.” Importantly, Dr Wang and Mr Smith determined the exact gene affected by this virus – a gene called CHL1. “Pinpointing this gene represents a major step forward in understanding exactly how some viruses cause disease symptoms in susceptible organisms,” Dr Wang said. Until recently, scientists did not fully understand why viruses only affected a small range of host organisms. This discovery shows that the accompanying satellite gene of CMV must directly match the host plant’s genes to cause the yellowing disease. When the viral satellite’s genes match the host plant’s genes, the satellite genes ‘lock’ onto and slice the host’s genes, preventing the host from forming green chlorophyll pigment. “Think of it as like doing up a zipper on your jacket – two opposing but different sections have to come together for it to work,” Dr Wang said. “So one half of the ‘zipper’ genes come from the virus and the other half of the genes from the host, and when they match up the virus causes disease.” This finding means researchers can focus on finding genes in viruses that match known genetic sequences in plants, and this can help to reveal the cause of diseases by other viruses. Knowing how CMV causes symptoms, Dr Wang and Mr Smith also experimented to see if they could block the viral disease in plants. They created specially altered plants with an extra copy of the chlorophyll-producing gene. This gene had been changed so that it no longer matched the viral gene, allowing the plants to produce the green chlorophyll Media Resources Images and audio available at: Major breakthrough on how viruses infect plants Background information available at: Fighting disease, pests and weeds Sound grab of CSIRO Plant Industry scientist, Dr Ming-Bo Wang. Quote one Options: Download (mp3) Sound grab of CSIRO Plant Industry scientist, Dr Ming-Bo Wang. Quote two Options: Download (mp3) Sound grab of CSIRO Plant Industry scientist, Neil Smith Quote one Options: Download (mp3) Sound grab of CSIRO Plant Industry scientist, Neil Smith Quote two Options: Download (mp3) Contact Information Science Contact Dr Ming-Bo Wang Senior Research Scientist Plant Industry Phone: 61 2 6246 5197 Email: [email protected] Media Contact Ms Ilaria Catizone (MSc) Communication Officer Plant Industry Phone: 61 2 6246 5485 Alt Phone: 61 4 1765 4633 Search Explore CSIRO Partner Media Events Education Publications Careers Contact Home > Media Major breakthrough on how viruses infect plants (Media Release) http://www.csiro.au/news/How-viruses-infect-plants.html 1 of 2 8/25/2011 11:55 PM

Major Breakthrough on How Viruses Infect Plants (Media Release)

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Media Release

Tobacco plants affected bythe virus and resistant to it.(Carl Davies CSIRO)

“Pinpointing this gene

represents a major step

forward in understanding

exactly how some viruses

cause disease symptoms

in susceptible organisms”

Dr Ming-Bo Wange,

CSIRO

Major breakthrough on howviruses infect plants

Reference: 11/57

CSIRO plant scientists have shed light on a

problem that has puzzled researchers since the

first virus was discovered in 1892 – how exactly

do they cause disease?

14 July 2011

In a major breakthrough that helps us better understand

how viruses cause diseases in plants – and potentially in

animals and humans – Dr Ming-Bo Wang and Neil Smith

of CSIRO Plant Industry have revealed a genetic mechanism that enables viral

organisms to infect hosts and cause diseases.

“Cucumber Mosaic Virus (CMV) is a common, destructive virus that affects a wide range

of food crops and ornamental plants,” Dr Wang said.

“What we found was that CMV, accompanied by a special type of viral particle called a

‘satellite’, causes its distinctive yellowing symptoms in plants by slicing a gene that

makes chlorophyll, the green pigment in leaves. By preventing the production of

chlorophyll, the virus causes the leaves to become partially or entirely yellowed which

dramatically affects growth and productivity.”

Importantly, Dr Wang and Mr Smith determined the exact gene affected by this virus –

a gene called CHL1.

“Pinpointing this gene represents a major step

forward in understanding exactly how some

viruses cause disease symptoms in susceptible

organisms,” Dr Wang said.

Until recently, scientists did not fully understand

why viruses only affected a small range of host

organisms. This discovery shows that the

accompanying satellite gene of CMV must directly

match the host plant’s genes to cause the

yellowing disease.

When the viral satellite’s genes match the host plant’s genes, the satellite genes ‘lock’

onto and slice the host’s genes, preventing the host from forming green chlorophyll

pigment.

“Think of it as like doing up a zipper on your jacket – two opposing but different

sections have to come together for it to work,” Dr Wang said.

“So one half of the ‘zipper’ genes come from the virus and the other half of the genes

from the host, and when they match up the virus causes disease.”

This finding means researchers can focus on finding genes in viruses that match known

genetic sequences in plants, and this can help to reveal the cause of diseases by other

viruses.

Knowing how CMV causes symptoms, Dr Wang and Mr Smith also experimented to see

if they could block the viral disease in plants. They created specially altered plants with

an extra copy of the chlorophyll-producing gene. This gene had been changed so that it

no longer matched the viral gene, allowing the plants to produce the green chlorophyll

Media Resources

Images and audio available at: Major

breakthrough on how viruses infect

plants

Background information available at:

Fighting disease, pests and weeds

Sound grab of CSIRO Plant Industryscientist, Dr Ming-Bo Wang.

Quote one

Options: Download (mp3)

Sound grab of CSIRO Plant Industryscientist, Dr Ming-Bo Wang.

Quote two

Options: Download (mp3)

Sound grab of CSIRO Plant Industryscientist, Neil Smith

Quote one

Options: Download (mp3)

Sound grab of CSIRO Plant Industryscientist, Neil Smith

Quote two

Options: Download (mp3)

Contact Information

Science Contact

Dr Ming-Bo Wang

Senior Research Scientist

Plant Industry

Phone: 61 2 6246 5197

Email: [email protected]

Media Contact

Ms Ilaria Catizone (MSc)

Communication Officer

Plant Industry

Phone: 61 2 6246 5485

Alt Phone: 61 4 1765 4633

SearchExplore CSIRO Partner Media Events Education Publications Careers Contact

Home > Media

Major breakthrough on how viruses infect plants (Media Release) http://www.csiro.au/news/How-viruses-infect-plants.html

1 of 2 8/25/2011 11:55 PM

pigment.

Remarkably, this small change in genetic makeup prevented the plants from becoming

yellow and diseased but did not change any other aspects of the plants’ growth, habit

or form.

This research was funded by CSIRO and the Australian Research Council (ARC) and will

be presented at the International Botanical Congress in Melbourne, 23-30 July 2011.

Former CSIRO Plant Industry researcher Dr Andrew Eamen also contributed to these

findings.

Read more media releases in our Media section.

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Major breakthrough on how viruses infect plants (Media Release) http://www.csiro.au/news/How-viruses-infect-plants.html

2 of 2 8/25/2011 11:55 PM