MEMO WCHR201710th World Congress for Hair ResearchVisiting old, find new

Department of Dermatology, Perelman School of Medicine, University of PennsylvaniaGeorge Cotsarelis, ProfessorProstaglandins and androgenetic alopecia.Speaker1

Centre for Cell Biology and Cutaneous Research, Blizard Institute, Queen Mary University London.Mike Philpott, Professor

Revisiting the Warburg effect and the importanceof hair follicle metabolism in health and disease.

Speaker2

Luncheon Seminar 1Wednesday Novemver 1,2017 12:15-13:20Date

Kyoto International Conference CenterAnnex Hall 2 (Main)

Location

Co-president of 10th World Congress for Hair ResearchSatoshi Itami, M.D., PhDChair

Centre for Skin Sciences, University of BradfordValerie Randall, ProfessorCo-Chair

Sponsored by

Satoshi Itami, M.D., PhDCo-president of 10th World Congress forHair Research

Education and Training1978 Graduated from Osaka University

Medical School1978-1980 M.D., Osaka University Medical

School

Appointments and Position1980-1985 Instructor, Department of

Dermatology, Oita Medical University

1982-1984 Research Fellow, Department of DermatologyUniversity of Miami, USA

1985 Awarded the degree of PhD from Osaka University

1985-1993 Assistant professor, Department of Dermatology, Oita Medical University

1993-1995 Associate Professor, Department of Dermatology, Oita Medical University

1995-2005 Associate Professor, Department of Dermatology, Course of Molecular Medicine, Graduate School of Medicine, Osaka University

2006- Endowed Chair Proffessor, Department of Regenerative Dermatology, Graduate School of Medicine, Osaka University

Research Focus1.Transgenic mice model for skin diseases2.Androgen action in skin3.Signal transduction in keratinocytes and dermal papilla cells

Professor Val Randall is Professor of Skin Sciences in the Centre for Skin Sciences, University of Bradford, UK, Fellow of the Institute of Biomedical Science and Fellow of the Royal Society of Biology. Her research interests in endocrinology and skin sciences developed while carrying out her PhD on endocrine regulation of sebaceous glands with Professor John Ebling, University of Sheffield. They were extended during postdoctoral positions with Professor Ebling studying hirsutism and seasonal changes in human hair growth and with Professor Ian Mainwaring, University of Leeds, on androgens and the prostate. As an independent academic her research has focussed on hair follicle biology, particularly now the regulation of human hair growth by androgens and prostanoids. Her research has been supported by both the Medical and Biotechnology & Biological Sciences Research Councils, charities and international industry. It has resulted in 3 edited books, numerous publications and invited book chapters and 21 research student graduations.

Valerie Randall, ProfessorCentre for Skin Sciences,University of Bradford

Mike Philpott, Professor

Centre for Cell Biology and Cutaneous Research,Blizard Institute, Queen Mary University London.

George Cotsarelis, Professor

Department of Dermatology, University of Pennsylvania

Prostaglandin pathways have major effects on

mouse and human hair growth. Prostaglandin D2

(PGD2) and its synthesizing enzyme, PGD2

synthase, are present at higher levels in balding

ve r su s n on - b a l d i ng s c a l p i n men w i t h

androgenetic alopecia. In a mouse model, PGD2

inhibits hair growth via CRTH2/ PTGDR2, one of

two PGD2 receptors, leading us to hypothesize that

PTGDR2 is the key receptor mediating the hair

growth inhibitory activity of PGD2 in human

follicles. We tested several pharmacological

PTGDR2 antagonists for their anti-PGD2 activity on

human hair growth in vitro and found that

PTGDR2 antagonists reversed the growth

inhibition mediated by PGD2, in a dose-dependent

manner, by reducing PGD2-triggered apoptosis

and maintaining keratinocyte proliferation.

Topical administration of PGD2 to mice resulted in

shortening of the anagen phase and accelerated

entry into catagen, while applying a PTGDR2

antagonist to mice extended anagen phase,

resulting in longer hair. RNA-Seq analysis on

cultured human hair follicles showed decreased

expression of hair follicle progenitor cell markers,

such as CD34 and K19, in the PGD2 treated group.

FACS analysis of mouse sk in cel ls showed

decreased Ki67-positive cells in the secondary hair

germ population prior to anagen re-entry in PGD2

treated mice. These results suggest that PGD2

suppresses the activation of the secondary germ/

hair progenitor cells . Our findings fur ther

underscore the key role of PGD2 in regulating hair

growth and indicate that pharmacological

antagonism of PTGDR2 may be an effective

approach in preventing and/or treating alopecia in

patients sensitive to PGD2.

George Cotsarelis, M.D serves as the Milton B. Hartzell Professor and Chairman of the Department of Dermatology at the University of Pennsylvania School of Medicine. His research focuses on epithelial stem cells, hair follicle biology, wound healing and skin regeneration.

Hair follicles (HF) are dynamic mini-organs with

high demand for energy and biosynthesis

precursors. The HF mainly engages in aerobic

glycolysis and glutamine metabolism with

lactate generation despite the presence of

oxygen known as the Warburg effect. However,

t h e H F i s a l s o c a p a b l e o f o x i d a t i v e

phosphorylation. We propose that aerobic

glycolysis and glutaminolysis generate large

fluxes of metabol i tes for anabol ism and

generation of the hair fibre and that as such the

HF probably balances aerobic glycolysis ,

glutaminolysis and oxidative phosphorylation

and that differences in aerobic glycolysis and

oxidative phosphorylation between highly

p ro l i fe rat ive and d ifferent iat ion t i s sue

compartments of the hair foll icle may be

important regulators of hair follicle function. We

have also shown that dermal papilla cells from

balding scalp are much more sensitive to

ox idat i ve s t ress and ROS than DP f rom

non-balding scalp and have proposed that

imbalance of aerobic glycolysis and oxidative

phosphorylation may be an important factor in

hai r loss d isorders . F inal ly there i s now

increasing evidence that the outer root sheath

may in fact be the ‘powerhouse’ of the hair

follicle and we have proposed that glycogen

metabolism in the ORS may be involved in

maintaining high rates of cell proliferation in

hair follicle.

Mike Philpott D.Phil is Professor of Cutaneous Biology at the Centre for Cell Biology and Cutaneous Research, Blizard Institute, Queen Mary University London. His research focuses on in vitro models of human hair growth with a strong interest in hair follicle metabolism, basal cell carcinoma and the role of steroids in skin biology.

Revisiting the Warbureffectand the importanceof hair follicle metabolismin health and disease.

Prostaglandins andandrogenetic alopecia.

Chair

Co-Chair

Speaker1 Speaker2

Satoshi Itami, M.D., PhDCo-president of 10th World Congress forHair Research

Education and Training1978 Graduated from Osaka University

Medical School1978-1980 M.D., Osaka University Medical

School

Appointments and Position1980-1985 Instructor, Department of

Dermatology, Oita Medical University

1982-1984 Research Fellow, Department of DermatologyUniversity of Miami, USA

1985 Awarded the degree of PhD from Osaka University

1985-1993 Assistant professor, Department of Dermatology, Oita Medical University

1993-1995 Associate Professor, Department of Dermatology, Oita Medical University

1995-2005 Associate Professor, Department of Dermatology, Course of Molecular Medicine, Graduate School of Medicine, Osaka University

2006- Endowed Chair Proffessor, Department of Regenerative Dermatology, Graduate School of Medicine, Osaka University

Research Focus1.Transgenic mice model for skin diseases2.Androgen action in skin3.Signal transduction in keratinocytes and dermal papilla cells

Professor Val Randall is Professor of Skin Sciences in the Centre for Skin Sciences, University of Bradford, UK, Fellow of the Institute of Biomedical Science and Fellow of the Royal Society of Biology. Her research interests in endocrinology and skin sciences developed while carrying out her PhD on endocrine regulation of sebaceous glands with Professor John Ebling, University of Sheffield. They were extended during postdoctoral positions with Professor Ebling studying hirsutism and seasonal changes in human hair growth and with Professor Ian Mainwaring, University of Leeds, on androgens and the prostate. As an independent academic her research has focussed on hair follicle biology, particularly now the regulation of human hair growth by androgens and prostanoids. Her research has been supported by both the Medical and Biotechnology & Biological Sciences Research Councils, charities and international industry. It has resulted in 3 edited books, numerous publications and invited book chapters and 21 research student graduations.

Valerie Randall, ProfessorCentre for Skin Sciences,University of Bradford

Mike Philpott, Professor

Centre for Cell Biology and Cutaneous Research,Blizard Institute, Queen Mary University London.

George Cotsarelis, Professor

Department of Dermatology, University of Pennsylvania

Prostaglandin pathways have major effects on

mouse and human hair growth. Prostaglandin D2

(PGD2) and its synthesizing enzyme, PGD2

synthase, are present at higher levels in balding

ve r su s n on - b a l d i ng s c a l p i n men w i t h

androgenetic alopecia. In a mouse model, PGD2

inhibits hair growth via CRTH2/ PTGDR2, one of

two PGD2 receptors, leading us to hypothesize that

PTGDR2 is the key receptor mediating the hair

growth inhibitory activity of PGD2 in human

follicles. We tested several pharmacological

PTGDR2 antagonists for their anti-PGD2 activity on

human hair growth in vitro and found that

PTGDR2 antagonists reversed the growth

inhibition mediated by PGD2, in a dose-dependent

manner, by reducing PGD2-triggered apoptosis

and maintaining keratinocyte proliferation.

Topical administration of PGD2 to mice resulted in

shortening of the anagen phase and accelerated

entry into catagen, while applying a PTGDR2

antagonist to mice extended anagen phase,

resulting in longer hair. RNA-Seq analysis on

cultured human hair follicles showed decreased

expression of hair follicle progenitor cell markers,

such as CD34 and K19, in the PGD2 treated group.

FACS analysis of mouse sk in cel ls showed

decreased Ki67-positive cells in the secondary hair

germ population prior to anagen re-entry in PGD2

treated mice. These results suggest that PGD2

suppresses the activation of the secondary germ/

hair progenitor cells . Our findings fur ther

underscore the key role of PGD2 in regulating hair

growth and indicate that pharmacological

antagonism of PTGDR2 may be an effective

approach in preventing and/or treating alopecia in

patients sensitive to PGD2.

George Cotsarelis, M.D serves as the Milton B. Hartzell Professor and Chairman of the Department of Dermatology at the University of Pennsylvania School of Medicine. His research focuses on epithelial stem cells, hair follicle biology, wound healing and skin regeneration.

Hair follicles (HF) are dynamic mini-organs with

high demand for energy and biosynthesis

precursors. The HF mainly engages in aerobic

glycolysis and glutamine metabolism with

lactate generation despite the presence of

oxygen known as the Warburg effect. However,

t h e H F i s a l s o c a p a b l e o f o x i d a t i v e

phosphorylation. We propose that aerobic

glycolysis and glutaminolysis generate large

fluxes of metabol i tes for anabol ism and

generation of the hair fibre and that as such the

HF probably balances aerobic glycolysis ,

glutaminolysis and oxidative phosphorylation

and that differences in aerobic glycolysis and

oxidative phosphorylation between highly

p ro l i fe rat ive and d ifferent iat ion t i s sue

compartments of the hair foll icle may be

important regulators of hair follicle function. We

have also shown that dermal papilla cells from

balding scalp are much more sensitive to

ox idat i ve s t ress and ROS than DP f rom

non-balding scalp and have proposed that

imbalance of aerobic glycolysis and oxidative

phosphorylation may be an important factor in

hai r loss d isorders . F inal ly there i s now

increasing evidence that the outer root sheath

may in fact be the ‘powerhouse’ of the hair

follicle and we have proposed that glycogen

metabolism in the ORS may be involved in

maintaining high rates of cell proliferation in

hair follicle.

Mike Philpott D.Phil is Professor of Cutaneous Biology at the Centre for Cell Biology and Cutaneous Research, Blizard Institute, Queen Mary University London. His research focuses on in vitro models of human hair growth with a strong interest in hair follicle metabolism, basal cell carcinoma and the role of steroids in skin biology.

Revisiting the Warbureffectand the importanceof hair follicle metabolismin health and disease.

Prostaglandins andandrogenetic alopecia.

Chair

Co-Chair

Speaker1 Speaker2

Satoshi Itami, M.D., PhDCo-president of 10th World Congress forHair Research

Education and Training1978 Graduated from Osaka University

Medical School1978-1980 M.D., Osaka University Medical

School

Appointments and Position1980-1985 Instructor, Department of

Dermatology, Oita Medical University

1982-1984 Research Fellow, Department of DermatologyUniversity of Miami, USA

1985 Awarded the degree of PhD from Osaka University

1985-1993 Assistant professor, Department of Dermatology, Oita Medical University

1993-1995 Associate Professor, Department of Dermatology, Oita Medical University

1995-2005 Associate Professor, Department of Dermatology, Course of Molecular Medicine, Graduate School of Medicine, Osaka University

2006- Endowed Chair Proffessor, Department of Regenerative Dermatology, Graduate School of Medicine, Osaka University

Research Focus1.Transgenic mice model for skin diseases2.Androgen action in skin3.Signal transduction in keratinocytes and dermal papilla cells

Professor Val Randall is Professor of Skin Sciences in the Centre for Skin Sciences, University of Bradford, UK, Fellow of the Institute of Biomedical Science and Fellow of the Royal Society of Biology. Her research interests in endocrinology and skin sciences developed while carrying out her PhD on endocrine regulation of sebaceous glands with Professor John Ebling, University of Sheffield. They were extended during postdoctoral positions with Professor Ebling studying hirsutism and seasonal changes in human hair growth and with Professor Ian Mainwaring, University of Leeds, on androgens and the prostate. As an independent academic her research has focussed on hair follicle biology, particularly now the regulation of human hair growth by androgens and prostanoids. Her research has been supported by both the Medical and Biotechnology & Biological Sciences Research Councils, charities and international industry. It has resulted in 3 edited books, numerous publications and invited book chapters and 21 research student graduations.

Valerie Randall, ProfessorCentre for Skin Sciences,University of Bradford

Mike Philpott, Professor

Centre for Cell Biology and Cutaneous Research,Blizard Institute, Queen Mary University London.

George Cotsarelis, Professor

Department of Dermatology, University of Pennsylvania

Prostaglandin pathways have major effects on

mouse and human hair growth. Prostaglandin D2

(PGD2) and its synthesizing enzyme, PGD2

synthase, are present at higher levels in balding

ve r su s n on - b a l d i ng s c a l p i n men w i t h

androgenetic alopecia. In a mouse model, PGD2

inhibits hair growth via CRTH2/ PTGDR2, one of

two PGD2 receptors, leading us to hypothesize that

PTGDR2 is the key receptor mediating the hair

growth inhibitory activity of PGD2 in human

follicles. We tested several pharmacological

PTGDR2 antagonists for their anti-PGD2 activity on

human hair growth in vitro and found that

PTGDR2 antagonists reversed the growth

inhibition mediated by PGD2, in a dose-dependent

manner, by reducing PGD2-triggered apoptosis

and maintaining keratinocyte proliferation.

Topical administration of PGD2 to mice resulted in

shortening of the anagen phase and accelerated

entry into catagen, while applying a PTGDR2

antagonist to mice extended anagen phase,

resulting in longer hair. RNA-Seq analysis on

cultured human hair follicles showed decreased

expression of hair follicle progenitor cell markers,

such as CD34 and K19, in the PGD2 treated group.

FACS analysis of mouse sk in cel ls showed

decreased Ki67-positive cells in the secondary hair

germ population prior to anagen re-entry in PGD2

treated mice. These results suggest that PGD2

suppresses the activation of the secondary germ/

hair progenitor cells . Our findings fur ther

underscore the key role of PGD2 in regulating hair

growth and indicate that pharmacological

antagonism of PTGDR2 may be an effective

approach in preventing and/or treating alopecia in

patients sensitive to PGD2.

George Cotsarelis, M.D serves as the Milton B. Hartzell Professor and Chairman of the Department of Dermatology at the University of Pennsylvania School of Medicine. His research focuses on epithelial stem cells, hair follicle biology, wound healing and skin regeneration.

Hair follicles (HF) are dynamic mini-organs with

high demand for energy and biosynthesis

precursors. The HF mainly engages in aerobic

glycolysis and glutamine metabolism with

lactate generation despite the presence of

oxygen known as the Warburg effect. However,

t h e H F i s a l s o c a p a b l e o f o x i d a t i v e

phosphorylation. We propose that aerobic

glycolysis and glutaminolysis generate large

fluxes of metabol i tes for anabol ism and

generation of the hair fibre and that as such the

HF probably balances aerobic glycolysis ,

glutaminolysis and oxidative phosphorylation

and that differences in aerobic glycolysis and

oxidative phosphorylation between highly

p ro l i fe rat ive and d ifferent iat ion t i s sue

compartments of the hair foll icle may be

important regulators of hair follicle function. We

have also shown that dermal papilla cells from

balding scalp are much more sensitive to

ox idat i ve s t ress and ROS than DP f rom

non-balding scalp and have proposed that

imbalance of aerobic glycolysis and oxidative

phosphorylation may be an important factor in

hai r loss d isorders . F inal ly there i s now

increasing evidence that the outer root sheath

may in fact be the ‘powerhouse’ of the hair

follicle and we have proposed that glycogen

metabolism in the ORS may be involved in

maintaining high rates of cell proliferation in

hair follicle.

Mike Philpott D.Phil is Professor of Cutaneous Biology at the Centre for Cell Biology and Cutaneous Research, Blizard Institute, Queen Mary University London. His research focuses on in vitro models of human hair growth with a strong interest in hair follicle metabolism, basal cell carcinoma and the role of steroids in skin biology.

Revisiting the Warbureffectand the importanceof hair follicle metabolismin health and disease.

Prostaglandins andandrogenetic alopecia.

Chair

Co-Chair

Speaker1 Speaker2

MEMO WCHR201710th World Congress for Hair ResearchVisiting old, find new

Department of Dermatology, Perelman School of Medicine, University of PennsylvaniaGeorge Cotsarelis, ProfessorProstaglandins and androgenetic alopecia.Speaker1

Centre for Cell Biology and Cutaneous Research, Blizard Institute, Queen Mary University London.Mike Philpott, Professor

Revisiting the Warburg effect and the importanceof hair follicle metabolism in health and disease.

Speaker2

Luncheon Seminar 1Wednesday Novemver 1,2017 12:15-13:20Date

Kyoto International Conference CenterAnnex Hall 2 (Main)

Location

Co-president of 10th World Congress for Hair ResearchSatoshi Itami, M.D., PhDChair

Centre for Skin Sciences, University of BradfordValerie Randall, ProfessorCo-Chair

Sponsored by

MEMO WCHR201710th World Congress for Hair ResearchVisiting old, find new

Department of Dermatology, Perelman School of Medicine, University of PennsylvaniaGeorge Cotsarelis, ProfessorProstaglandins and androgenetic alopecia.Speaker1

Centre for Cell Biology and Cutaneous Research, Blizard Institute, Queen Mary University London.Mike Philpott, Professor

Revisiting the Warburg effect and the importanceof hair follicle metabolism in health and disease.

Speaker2

Luncheon Seminar 1Wednesday Novemver 1,2017 12:15-13:20Date

Kyoto International Conference CenterAnnex Hall 2 (Main)

Location

Co-president of 10th World Congress for Hair ResearchSatoshi Itami, M.D., PhDChair

Centre for Skin Sciences, University of BradfordValerie Randall, ProfessorCo-Chair

Sponsored by