Upload
akil
View
215
Download
0
Embed Size (px)
Citation preview
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
1/205
Unit 1
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
2/205
Microscopes
Prokaryotic cells
Cell fractionation
Scientists use optical microscopes and transmissionelectron microscopes (TEMs) toinvestigate cell structure. Explain the advantages andthe limitations of using a TEM
to investigate cell structure.
Advantages:1 Small objects can be seen;
2 TEM has high resolution;3 Wavelength of electrons shorter;imitations:! "annot loo# at living cells;$ Must be in a vacuum;% Must cut section & thin s'ecimen;( )re'aration ma* create artefact
+ ,oes not 'roduce colour image;
The structure of a cholera bacterium is different from thestructure of an epithelial cell from the small intestine.Describe how the structure of a cholera bacterium is
Different.
1 "holera bacterium is 'ro#ar*ote;2 ,oes not have a nucleus&nuclear envelo'e& has ,-A freein c*to'lasm&has loo' of ,-A;3 and ! An* t.o from-o membrane/bound organelles&no mitochondria & no golgi&no endo'lasmic reticulum&etc;$ Small ribosomes onl*;
% and ( An* t.o from"a'sule&flagellum&'lasmid & cell .all&etc;
Measuring the size of an object under a
microscope
Measure with an eyepiece graticule
Calibrate with the stage mcirometer (an object of a
known size)
Repeat and calculate an aerage
Explain the advantages and limitations of using a transmission
electron microscope to study cells.
1 TEM uses (beam of) electrons;
2 These have short avelength;! "llo high resolution#greater resolution#"llo more detail to
be seen#greater useful magnification;$ Electrons scattered (by molecules in air);% &acuum established;' annot examine living cells;
*ots of preparation#procedures used in preparing specimens# fixing#staining#sectioning;+ May alter appearance#result in artefacts;
Starting with some lettuce leaves,describe how you would obtain a sample of
undamaged chloroplasts. se your
knowledge of cell fractionation and
ultracentrifugation to answer this
!uestion.
1! Chop up (accept any reference to crude
breaking up)" #! Cold" (reduces
enzyme actiity)
$! %u&ered solution" (preents p' a&ecting
enzymes)! sotonic * same water potential" (preents
osmosis and possible lysis or shrinkage of
organelles)
+! ,ilter and centrifuge -ltrate"
.! Centrifuge supernatant"
/! 0t higher speed"
! Chloroplasts in (second) pellet"
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
3/205
2rokaryotic cells
and fractionation
The bacteria in the intestine are pro,aryotic cells. The
epithelial cells hich line the small intestine are eu,aryoticcells. -escribe the ays in hich pro,aryotic cells and
eu,aryotic cells differ
1 ro,aryotic cells do not have a nucleus # have genetic
material in cytoplasm;
2 -/" in loop # ring;! /ot associated ith proteins # do not have chromosomes #
chromatin # do not divide by mitosis;
$ 0maller ribosomes;
% /o membranebound organelles;
' 0uch as mitochondria # lysosomes # endoplasmic
reticulum # olgi # chloroplasts;
ro,aryotic cells may have mesosomes;
+ ro,aryotic cells smaller;
3 May be enclosed by capsule;
4o pro,aryotic cell is the same and different to a eu,aryotic cell
cytoplasm;
ribosomes;
phospholipid membranes # cell membrane # semipermeable
membrane; 2 max
(accept folded membrane for two marks)
(ii)
(it = bacterium)
cell all;
capsule;
flagellum;mesosome;
no nucleus # nuclear membrane # -/" free;
no mitochondria;
(accept no membrane-bound organelles if neither nucleus
nor mitochondria mark scored)
no microvilli;
no olgi;
no E5;
60#smaller ribosomes;
arts of the pro,aryotic cell
cell (surface) membrane7
regulates entry#exit#selectively permeable;
8mesosome7
respiration#cell division;
cell all7(mechanical) protection#prevents (osmotic) lysis;
-slime layer#capsule7
protection (against e.g. antibiotics);
Eflagellum7
movement of cell;
9 -/" molecule#bacterial chromosome7
genetic information;
ive two factors hich affect the ability ofbacteria to cause a disease.
pathogenicity # toxicity of products;
site of infection;
invasiveness;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
4/205
Microscopes
,ractionation
*abelled antibodies and an electron microscope can be used
to produce images locating proteins on the surface oforganelles7 but cannot be used to observe cross bridge
cycling in muscle cells. Explain hy.
1. e.m. gives high resolution;
2. due to short avelength of electrons;
!. antibodies attach specifically to target proteins;$. gold particles are electron dense;
%. electrons must pass through a vacuum;
'. material must be dead # fixed for e.m.;
. crossbridge cycling re:uires living cells #
metabolism # named
aspecte.g. "T synthesis;
0tarting ith some lettuce leaves7 describe hoyou ould obtain a sample of undamaged
chloroplasts. se your ,noledge of cell
fractionation and ultracentrifugation to
anser this :uestion.
1. hop up (accept any reference to crude
brea,ing up);
2. old;
!. 8uffer solution;
$.
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
5/205
2rokaryotic cells
and iruses and
microscopes
-escribe the ays in hich pro,aryotic cells and eu,aryotic
cells differ
ro,aryotic cells do not have a nucleus # have genetic
materialin cytoplasm;
-/" in loop # ring;/ot associated ith proteins # do not have chromosomes #
chromatin # do not divide by mitosis;
0maller ribosomes;/o membranebound organelles;0uch as mitochondria # lysosomes # endoplasmic reticulum #
olgi # chloroplasts;
ro,aryotic cells may have mesosomes;
ro,aryotic cells smaller;May be enclosed by capsule;
-efine resolving poer and state hy it is bteter forelectron microscopes than light
(i) "bility to distinguish points(close together); 1
(ii) Electrons have a
shorter avelength;
Explain ho viruses cause damage to cells.
uses # brea,s up # digests host nuclear # geneticmaterial (allow references
made to #$A %&$A instead of nuclear %genetic)'
virus -/" # genetic material inserted into hosts -/" #
chromosome
# genetic material;
host cells amino acids are used to synthesi@e viralproteins;
cell lysis;
by en@yme (produced by expressing a virus gene);
toxin production;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
6/205
Cell
membranes
Describe the fluid-mosaic structure of a cell surface
membrane.(5)
hospholipids and proteins;
hospholipid bilayer;
"rrangement of phospholipid molecules ATails to
tailsB;
A9loatingB(protein) molecules # molecules can move in
membrane;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
7/205
Cell
membrane
-escribe the structure of a cell membrane.
-ouble layer of phospholipid molecules;
-etail of arrangement of phospholipids;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
8/205
Cell
membranes
-escribe ho proteins are arranged in a plasma membrane
and the part they play in transporting substances into and outof cells.
1 0ome proteins pass right through membrane;
2 0ome proteins associated ith one layer;
!
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
9/205
Cell
membranes
-escribe ho proteins are arranged in a plasma
membrane and the part they play in transporting
substances into and out of cells.
1 0ome proteins pass right through membrane;
2 0ome proteins associated ith one layer;
!
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
10/205
+arrier proteins involved ith facilitated diffusion;
Cell
membranes
-escribe ho proteins are arranged in a plasma
membrane and the part they play in transportingsubstances into and out of cells.
0ome proteins pass right through membrane;
2 0ome proteins associated ith one layer;
!
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
11/205
5nzymes
Explain ho pepsin is inactivated by the high p4 in
the small intestine.
4igh p4 denatures en@yme# alters charge on active
site;8rea,s bonds;
"lters tertiary structure of en@yme molecule;hanges shape of active site;
"ctive site can no longer bind ith# form E0complexes ith# is no
longer complementary to substrate;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
12/205
"nzymes
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
13/205
"nzymes
xplain what happens to an en!yme molecule when it is
denatured by high temperature.
orrectly named bonds bro,en # ater removed;
tertiary # globular shape of en@yme changed;
shape of active site affected;
Temperature has a mar,ed effect on blood p4. "t ! Gblood plasma has a p4 of .$ but at a temperature of 2% G7
the p4 is '.3.
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
14/205
"nzymes
Explain ho a substrate is bro,en don by the en@yme.
0ubstrate enters active site;
omplimentary shapes # *oc, and Hey;
(8inding) to form en@ymesubstrate complex;
*oering of activation energy;
onformational # shape change;
8rea,ing of bonds in substrate;
roducts no longer fit active site and so are released;
se your ,noledge of the tertiary structure of en@ymes to
explain ho a non competitive inhibitor could reduce the
rate of an en@yme controlled reaction.
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
15/205
"nzymes
Explain ho the shape of an en@yme molecule is related to
its function.
specific !- tertiary structure#shape;
substrate complementary shape; (re+ect same shape)
substrate (can bind) to active site# can fit into each active
site;
decreasing the p4 affects carbohydrase activity.
(decrease in p4) increases 4Iions#protons;
% attach#attracted to amino acids;
' hydrogen#ionic bonds disrupted#bro,en;
denatures en@yme # changes tertiary structure;
+ changes shape#charge of active site;
3 active site#en@yme unable to combine#fit ith
starch#en@ymesubstrate
complex no longer able to form decreases rate of brea,donof starch#rate of reaction #carbohydrase activity;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
16/205
"nzymes
-escribe ho molecular shape is important in explaining the
ay in hich en@ymes may be affected by inhibitors.
"ctive site (of en@yme) has particular shape;
(
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
17/205
5nzymes
-escribe ho molecular shape is important in explaining the
ay in hich en@ymes may be affected by inhibitors.(')
1 "ctive site (of en@yme) has particular shape;
2 (
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
18/205
5nzymes
-escribe ho the condensation reaction can be catalysed by
an en@yme.
en@yme has an active site;
ith a complementary shape to the substrate molecules;
en@ymesubstrate complex formed;
loering the (activation) energy for the reaction;
glycosidic bond formed#bringing together hydroxyl
groups#ater
molecule removed;
products leave the active site;
en@yme unchanged;
Explain ho substrates are bro,en don by the en@yme.
0ubstrate enters active site;
omplimentary shapes # *oc, and Hey;
(8inding) to form en@ymesubstrate complex;
*oering of activation energy;
onformational # shape change;
8rea,ing of bonds in substrate;
roducts no longer fit active site and so are released;
Explain ho amylase ma,es it possible for starch to be
digested at body temperature.
"ctivation energy reduced;
starch attached to active site # formation of en@ymesubstrate
complex;
less energy re:uired to bring (substrate) molecules together# to brea, bonds;
reaction occurs in small(er) steps;
change in shape of en@yme molecule (induced fit) brings
molecules
together # allos bonds to brea, # causes overlapping ofelectron
orbits of substrates.
-escribe and explain ho an increase in temperature affects
the rate of an en@yme controlled reaction.
Temperature
5ate of reaction increases;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
19/205
"ctive site altered#substrate cannot bind#fit#
5nzymes
Many reactions ta,e place in living cells at temperatures far
loer than those re:uired for the same reactions in a
laboratory. Explain ho en@ymes enable this to happen.
loers activation energy;
relevant mechanism e! g! brings molecules close together %reaction in smaller
steps % change in charge distribution % proton donation or
acceptance % induced
fit ensuring substrates brought in correct seuence'
including relevant reference to active site;
Expalin ho decreasing the p4 affects carbohydrase activity.
(decrease in p4) increases 4Iions#protons;
% attach#attracted to amino acids;
' hydrogen#ionic bonds disrupted#bro,en;
denatures en@yme # changes tertiary structure;
+ changes shape#charge of active site;
3 active site#en@yme unable to combine#fit ith
starch#en@ymesubstrate
complex no longer able to form;
decreases rate of brea,don ofstarch#rate of reaction
#carbohydrase activity;
Explain ho the shape of an en@yme molecule is
related to its function.
specific !- tertiary structure#shape;
substrate complementary shape; (re+ect same shape)
substrate (can bind) to active site# can fit into each
active site;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
20/205
%iological
molecules
Describe a chemical test you could carry out to show that
a piece of coconut contains lipids.()
(rush in) ethanol # alcohol;
"dd (to) ater (3rder of adding is critical for this point);
Emulsion # hite colour
Describe how you would use a biochemical test to show
that a sample contained reducing sugar.(,)
8enedictBs and heat;
reen # yello # orange # red # bron
#o not credit unualified references to water baths
Describe how the se+uence of amino acids in part of
a protein from a persons ears could enable this
protein to act as an en!yme inhibitor.(6)
1 0e:uence of amino acids gives shape;
2 This is tertiary structure;
! 4as similar shape to substrate;
$ 9its # competes for active site;
% 9its at site other than active site;
' -istorting active site;
Therefore substrate ill not fit (active site);
Describe how you would use a biochemical test to show
that a solution contained protein.
8iuret # al,ali I copper sulphate;
*ilac#purple#mauve#violet;
xplain what is meant by a polymer.
(Molecule) made up of many identical#similar
molecules#monomers#
subunits;
Explain ho proteins are suited for their roles as receptor
molecules.
Many different sorts of proteins;
-ifferent primary structures#se:uences of amino acids;
Tertiary structure;
0hape; alloing formation of receptor#binding site#site into
hich
substance#substrate fits;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
21/205
#ipid$protein carbs
?ith reference to named parts of the diagram7 explain the
difference beteen the terms=
triglyceride andphospholipid;
hospholipid has (one) phosphate # hosphoric acid;2
replacing fatty acid;
saturated and unsaturated.
0aturated F all valencies of filled # saturated ith
hydrogen # all (F)
single bonds # no double bonds;
fatty acid 1 is saturated#fatty acids 2 and ! are unsaturated;
-escribe ho a saturated fatty acid differs in molecular
structure from an unsaturated fatty acid.
absence of a double bond;
in the (hydrocarbon) chain;
unable to accept more hydrogen # saturated ith hydrogen;
Explain ho the structure of fibrous proteins is related to
their functions.
*ong chains of aa;
9olding of chain into a coil # folds # helix # pleated sheet;
"ssociation of several polypeptide chains together;
9ormation of fibres # sheets explained;
2
4 bonds # -isulphide bonding (,n context);
9ibres provide strength (and flexibility);
0heets provide flexibility;
Example e.g. ,eratin in hair7 collagen in bone; (4567 be in
context)
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
22/205
%iological
molecules
-escribe the structure of an amino acid molecule and
explain ho amino acids lin, together.
1 "mino acid based on carbon ith four groups attached;
2 "mino# /42and carboxyl # 4;
! 5group# side chain I hydrogen;$ 5group differs from one amino acid to another;
% "mino acids >oined by condensation;
' 8ond formed beteen /42and 4;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
23/205
%iochemical tests
-escribe ho you could use 8enedictBs reagent
to test a urine sample for the presence of
glucose.
"dd (8enedictBs) reagent (to urine sample) and heat #
heat the mixture;
red# bron# orange# green# yello;
describe a further biochemical test to find out ifsubstance D is a nonreducing sugar.
heat ith acid7 then neutralise # hydrolyse using
en@yme;
(heat) ith 8enedictBs (solution);
-escribe ho you ould use a biochemical test to
sho that a solution contained protei
8iuret # al,ali I copper sulphate;
*ilac#purple#mauve#violet;
-escribe a chemical test you could carry out to sho
that a piece of coconut contains lipids.
(rush in) ethanol # alcohol;
"dd (to) ater (3rder of adding is critical for thispoint);
Emulsion # hite colour;
-escribe ho standard solutions could be used
to estimate the concentration of reducing
sugar in the samples.
0ugar solutions of ,non # specificconcentrations;
Test each concentration ith 8enedictBs
solution;
use e:ual volumes of solutions # variablescontrolled;
Method of comparison7 e.g. compare colours7
mass of precipitate.
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
24/205
Cholera
Explain ho the effects of diarrhoea on the body can
be treated.
oral rehydration therapy#5T;
replaces lost ater and salts;
5
drin,ing large amounts of ater;
ith salts#minerals;
0uggest hy the cholera exotoxin is specific to the
epithelial cells of the small intestine.
receptor # proteins on membrane;
complementary shape of exotoxin;
-escribe the difference beteen an endotoxin and an
exotoxin.
endotoxins produced from the brea,don of bacteria
(cell alls);
(allow burst % l1se / do not allow decompose)
exotoxins secreted # released (from living cells) (notproduced);
endotoxins are lipopolysaccharides;
exotoxins are protein;
Explain ho an oral rehydration solution (50)
replaces ater lost by diarrhoea
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
25/205
Cholera
The structure of a cholera bacterium is different from the
structure of an epithelial cell from the small intestine.
-escribe ho the structure of a cholera bacterium is
different.
holera bacterium is pro,aryote;
-oes not have a nucleus#nuclear envelope# has -/" free in
cytoplasm#has loop of -/";! and $ "ny to from
/o membranebound organelles#no mitochondria # no
golgi#no
endoplasmic reticulum#etc;4aximum of . marks for points and 8!
%0mall ribosomes only;
' and "ny to from
apsule#flagellum#plasmid # cell all#etc;
6ral rehydration solutions (6R7) are used totreat diarrhoeal disease! 8hat does an 6R7consist of and how does it work9(+)
10 "ontains glucose&starch& carboh*drate & sugar;20 Sodium&salt;30 "o/trans'ort & s*m'ort;!0 Sodium and glucose ta#en u' from lumen;$0 o.ers .ater 'otential in cells& increases .ater'otential gradient;%0 Water ta#en u' b* osmosis
ive twoays in hich pathogens can cause disease
hen they enter the body of their host.
-amage#destruction of cells#tissues;
roduction of toxins;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
26/205
3igestion
Describe the role of the enzmes of the digestive sstem
in the complete brea!down of starch.
Am*lase;Starch to maltose:Maltase;Maltose to glucose;*drol*sis;
4f gl*cosidic bond;
Describe the processes involved in the absorption of the
products of starch digestion.
5lucose moves in .ith sodium into e'ithelial cell;6ia carrier&channel 'rotein&s*m'ort;Sodium removed from e'ithelial cell b* activetrans'ort&sodium/'otassium 'um';7nto blood;Maintaining lo. concentration of sodium in e'ithelial cell &maintaining sodium concentration gradient bet.een lumenand e'ithelial cell;5lucose moves into blood;
8* facilitated diffusion;
Describe and explain the roles of diffusion" facilitated diffusion
and acti&e transport in the absorption of digested food by the
ileum.
-iffusionmovement along # don concentration gradient;monoglycerides # micelles#fatty acids move into epithelial cells;
monoglycerides move from epithelium into blood;
chylomicrons move into lacteals # lymph;facilitated diffusionmovement along # don concentration gradient;reference to carrier # channel proteins;
monosaccharides or named # amino acids move into epithelial cells;active transportmovement against concentration gradient;
energy # "T re:uired;reference to carrier proteins;
monosaccharides or named # amino acids moved into epithelial cells;reference to codiffusion e.g. glucose and /al;monosaccharides or named # amino acids move into blood;
Describe how sugars are absorbed from the small intestine
into the blood of a mammal.
rinciples=
diffusion into capillaries;
active transport#facilitated diffusion involved;
"T used by active transport;
-etail=
disaccharidases#en@ymes in cell surface membrane;glucose #monomers#monosaccharides actively transported into
epithelial cells;
via protein carriers#channels (in membranes);
facilitated diffusion fromepithelial cell # toards blood;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
27/205
&igestion
xplain how the small intestine is adapted to its
function in the absorption of the products of
digestion.
*arge surface area provided by villi # microvilli;
long # folds increase surface area # time for absorption;
thin epithelium;
short diffusion pathay;
capillary netor, absorbs amino acids # sugars;
lacteal for absorption of digested fats;
Maintains a steep concentration gradient
mitochondria supply "T # energy for active transport;
carrier proteins (in membranes);
Describe how carbohydrate eaten as starch is digested to
produce glucose.
0tarch digested to maltose by amylase;
9ound in saliva; 0ecreted by pancreas;
Maltase converts maltose to glucose;
9ound in membranes of cells lining small intestine;
8oth reactions involve hydrolysis;
Describe how maltose in the small intestine is digested"
absorbed and transported to the li&er as glucose.
4ydrolysed by maltase;
Maltase en@ymes in membranes of epithelial cells of small
intestine;
lucose absorption involves diffusion;
"ssociated ith upta,e of sodium ions;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
28/205
&igestion
-escribe and explain the roles of diffusion7 facilitated diffusion andactive transport in the absorption of digested food by the ileum.
(allow general points provided correct molecule%particle involved)diffusion
movement along # don concentration gradient;monoglycerides # micelles#fatty acids move into epithelial cells;monoglycerides move from epithelium into blood;chylomicrons move into lacteals # lymph;
facilitated diffusionmovement along # don concentration gradient;
reference to carrier # channel proteins;monosaccharides or named # amino acids move into epithelial cells;active transportmovement against concentration gradient;
energy # "T re:uired;reference to carrier proteins;
monosaccharides or named # amino acids moved into epithelial cells;reference to codiffusion e.g. glucose and /al;monosaccharides or named # amino acids move into blood;
Describe the role of the en!ymes of the digesti&e system
in the complete breakdown of starch.
"mylase;
(0tarch) to maltose=
Maltase;
Maltose to glucose;
4ydrolysis;
(f) glycosidic bond;
Describe and explain how the small intestine is adapted
to increase the rate of absorption
many # pro>ecting villi (J) (no double penalt1 for microvilli);
large surface area (for absorption);
large#good blood supply # many capillaries#blood vessels;
maintains concentration gradients # efficient removal ofdigested products;
thin outer layer # blood vessels near to surface;
short diffusion pathay;
Describe the processes in&ol&ed in the absorption of the products
of starch digestion.
lucose moves in ith sodium (into epithelial cell);
&ia (carrier#channel) protein#symport;
0odium removed (from epithelial cell) by active transport#sodium
potassium pump;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
29/205
&igestion
'ow small intestine epithelia are adapted forabsorption
10 Microvilli;20 arge&increased surface area;30 Man* mitochondria;!0 Mitochondria&res'iration 'roduce AT) & release or'rovide energ* for active trans'ort;$0 "arrier 'roteins for active trans'ort;%0 "hannel & carrier 'roteins for facilitated diffusion;(0 "o/trans'ort of sodium ions and glucose or s*m'ort& carrier 'rotein for sodium ions and glucose;+0 Membrane/bound en9*mes digest disaccharides &'roduce glucose
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
30/205
#ifestyle
and &isease
Describe how atheroma may form and lead to a
myocardial infarction.(6)
1 fatty substance # foam cells # cholesterol in artery all #
under endothelium;2 formation of pla:ues # atherosclerosis # atheroma narros
lumen of artery;
! atheroma creates turbulence # damage to lining of artery;$ (turbulence) increases ris, of blood clot # embolus;% blood clot # thrombus brea,s off;
' (blood clot) lodges in coronary artery;
reduced blood supply to heart muscle;
+ reduced oxygen supply;3 leads to death of heart muscle;
igarette smoking and a diet high in saturated fat increase the
risk of myocardial infarction. xplain how.(6)
arbon monoxide combines ith haemoglobin#causes lessoxygen to be transported;-ecreases concentration of antioxidants in blood;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
31/205
#ifestyle
and &isease
Emphsema is another disease of the lungs. "eople withemphsema ma feel wea! and tired. Explain wh.
1 Alveoli brea# do.n & colla'se & ru'ture & .alls thic#en;2 ess surface area & increases diffusion distance & lessdiffusion;3 oss of elastin & elastic tissue & elastase involved;! Alveoli & lungs cannot recoil & s'ring bac# & have reduced
elasticit* & more difficult to e
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
32/205
#ifestyle
and &isease
Describe how altered D/% may lead to cancer.
1 (-/" altered by) mutation;
2 (mutation) changes base se:uence;
! of gene controlling cell groth # oncogene # that monitors
cell division;
$ of tumour suppressor gene;
% change protein structure # nonfunctional protein # proteinnot formed;
' (tumour suppressor genes) produce proteins that inhibit
cell division;
mitosis;
+ uncontrolled # rapid # abnormal (cell division);
3 malignant tumour;
xplain what is meant by malignant. (,)
(ancer L ) mass of cells that divide continuously #uncontrolled # faster;
(Malignant L ) can spread (to other body parts);
explain what is meant by a malignant tumour and
describe how exposure to cigarette smoke may result in
the formation of a malignant tumour. (6)
(5elative ris, of) lung cancer decreases the longer it is since
giving up smo,ing;(5elative ris, of) lung cancer increases ith the number of
cigarettes smo,ed per day;2
Mass of abnormal cells;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
33/205
#ifestyle
and disease
xplain how emphysema reduces the efficiency of gas
exchange in the lungs. %nd suggest two factors that could
increase risk other than smoking
alls of alveoli bro,en don # feer alveoli present;
smaller surface for diffusion;
5reduced elasticity;
ventilation restricted;5
scar tissue formed;less area for gas exchange # sloer gas exchange;
infection eg (chronic) bronchitis;
heredity;industrial pollution must contain reference to
inhalation of particles (dust);
xplain why atheroma may result in cardio&ascular
disease.
ea,en blood vessels may burst # aneurysm;
vessels narro;
blood pressure may rise;
blood clot may occur hich restricts or cuts off blood flo;
in coronary artery this leads to myocardial infarction # heart
attac, # angina;
in artery to brain this leads to stro,e;
Explain hy these factors increase the ris, of developing cardiovascular disease.salt7 smo,ing1at
blood cholesterol level increases;*-*s transport cholesterol in the blood;
*-*s deposit;cholesterol in arteries # atheroma formed;
blood pressure increased;(K)
Salt
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
34/205
#ifestyle
and disease
xplain how these effects of nicotine increase the risk of
cardio&ascular disease.
noradrenaline produced by 0/0;
stimulates 0"/;
increase in heart rate#cardiac output;
blood pressure increases;
increased ris, of cerebrovascular accident#stro,e;increased ris, of blood clot#thrombosis;
'he diet of a person can increase the riskof coronary heart disease. "(plain how.
10 Too much saturated fat& cholesterol in diet;20 7ncrease in ,& cholesterol in blood;30 Atheroma& fatt* de'osits& 'la>ues in arter* .alls;!0 =educes diameter of & bloc#s coronar* arteries;$0 ess o
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
35/205
)eart
Describe the parts played by the sinoatrial node (S%/) and the
atrio&entricular node (%2/) in controlling the heart beat.
10"/ initiates # sends heart beat;
2Myogenic # beats spontaneously # does not re:uire nerve impulse;
!5ate of beating influenced by nerves=
$?ave of electrical activity # impulses # excitation passes over
atrium;
%Triggers contraction of atrium;
'Electrical activity can only pass to ventricles # along bundle of
4is by ay of "&/
9ibrous tissue prevents passage elsehere;
+ -elay at "&/;
3 "llos blood to empty into ventricles # atria to empty;
Describe how the regular contraction of the atria and&entricles is initiated and coordinated by the heart itself.
(cardiac) muscle is myogenic;
sinoatrial node#0"/;
ave of depolarisation#impulses#electrical activity (across
atria);
initiates contraction of atria
atrioventricular node#"&/;
bundle of 4is#pur,yne tissue spreads impulse across
ventricles;
ventricles contract after atria#time delay enables ventricles to
fill;
he heart rate of a sleeping person is low. xplain how
ner&es supplying the heart may produce a low heart rate
in a sleeping person.
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
36/205
)eart
xplain how a rise in blood pressure results in a decrease
in the rate of heartbeat.
1 pressure receptors # baroreceptors # stretch receptors;
2 in aorta # carotid arteries # carotid sinus; (re+ect carotid
bod1)
! send impulses;
(re+ect signals % messages % electronic)$ to cardiovascular centre # medulla # cardioinhibitorycentre;
% send impulses;(once onl1)
' parasympathetic nerves # vagus; (accept inhibitor1 nerve)
to 0"/;
+ release of "h # inhibits 0"/ # decreases impulses from0"/;
3 decreases impulses to "&/ # decreased stimulation of"&/ #
decreases impulses from "&/;*ncreased intensity of e(ercise leads to an
increased heart rate. "(plain how.
10 4uence
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
37/205
&iseases
xplain the effect of smoking on blood pressure0 (,)
8ecause arteries cannot dilate # dilate less;
4eart must or, harder to force blood through;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
38/205
&isease
3hat is atheroma4 (,)
la:ue# fatty material# cholesterol# foam cells# lipoproteinbuild up;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
39/205
&iseases
Describe how atheroma is caused and how it may result in
a myocardial infarction. (6)
1. 4igh fat diet#high salt diet#lac, of exercise#age#gender;
7;3 risk factors for one mark
$ot h1pertension as this is given later
2. "theroma forms under endothelium#in artery all;
!. "theroma may narro lumen of artery;
$. "theroma increases blood pressure;%. "theroma promotes clotting;
'. -etails of effect of atheroma on clotting;
. 8lood clot lodges in coronary artery;
+. 5educed blood supply to heart muscle;
?! Reduced o4ygen*glucose supply leading to celldeath"
igarette smoking and a diet high in saturated fat increase the risk ofmyocardial infarction. xplain how.(6)
arbon monoxide combines ith haemoglobin#causes less
oxygen to be transported;
-ecreases concentration of antioxidants in blood;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
40/205
%reathing
Describe how muscles in the thorax (chest) cause
air to enter the lungs during breathing.
-iaphragm#intercostal muscles contract;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
41/205
%reathing
-escribe ho the medulla in the brain and the stretch
receptors in the lungs maintain the breathing rate hen the
body is at rest.
5espiratory centre in medulla;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
42/205
*mmunity
hagocytes and lysosomes are in&ol&ed in
destroying microorganisms. Describe how.
hagocytes engulf pathogens#microorganisms;
Enclosed in a vacuole # vesicle# phagosome;
*ysosomes have en@ymes;
That digest#hydrolyse
molecules#proteins#lipids#microorganism;
%n antigen in a &accine leads to the production of
antibodies. Describe the part played by 7
lymphocytes in this process.(5)
1 macrophages present antigens to 8 lymphocytes;
2 antigen binds to#is complementary to receptors onlymphocyte;
! binds to a specific lymphocyte;
$ lymphocytes become competent#sensitised;
% (8) lymphocytes reproduce by mitosis #(8)
lymphocytes cloned;
' plasma cells secrete antibodies;
Describe how these antibodies are produced in response to
foreign antigens.
antigens attach to macrophages # antigen presenting;
T lymphocytes activated by antigens;
helper T lymphocytes activate;
8 lymphocytes;specific cells (activated);
divide (by mitosis) # clone;
plasma cells # lymphocytes secrete antibodies;
(accept 7 cells% < cells as alternatives throughout)
%ntibodies are protein molecules. xplain why
protein molecules are particularly well suited to
carry out the role of antibodies.
large variety of different molecules;
range of shapes;
tertiary shape;
loc,s onto # complements specific antigen;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
43/205
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
44/205
*mmunity
Describe how 7-lymphocytes respond when antigens
stimulate them.
-ivide by mitosis # form clones;
produce plasma cells;
(plasma cells) ma,e antibodies;
(plasma cells) produce memory cells;
-ivide by mitosis # form clones; produce plasma cells; (plasmacells)
ma,e antibodies;
(plasma cells) produce memory cells;
'mmunisation programmes may use either attenuated ordead microorganisms. Suggest why there might be
problems for the patient when using these &accines .
rocess of ,illing organisms might not be 166O efficient;
live organisms might give rise to fullblon disease;
attenuated organisms are nonvirulent;
but might mutate to virulent forms;
immunity can decline booster in>ections re:uired;
named side effects7 eg allergies;
less effective due to changed antigens;
xplain the role of 7-lymphocytes and -lymphocytes in the
defence of the body against a &irus infection.
8 lymphocytes produce antibodies#involved in humoral response;T lymphocytes involved in cell mediated immunity;
Macrophages present antigens;(specific) 8 lymphocytes recognise#bind to antigen;increase in numbers by mitosis;produce plasma cells (hich ma,e antibodies);
antibodies bind to and clump# agglutinate virus;memory cells produced by 1
stexposure#cloned on 2
ndexposure;
T lymphocytes(helpers) produce lympho,ines#chemicals;hich aid 8 lymphocyte cloning;encourages phagocytes to engulf clumped virus;,iller T cells ,ill virus infected cells;
2accines protect against disease by stimulating theproduction of memory cells. Describe how memory cells
protect the body from disease.
n further exposure to same microorganism;
"ntigen recognised;
9aster response;
reater production of antibodies;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
45/205
*mmunity
8i&e two ways in which passi&e immunity differs from
acti&e immunity.
"ntibodies not produced by body;
/o memory cells;
0hortterm # not lifelong;
"ntibodies (or context established) donated by mother #
across placenta # in mil,;
3hat is &accination4ection of antigens#toxoids;
("ntigen from) attenuated microorganism#nonvirulent
microorganisms#deadmicroorganisms#isolated from microorganism;
0timulates the formation of memory cells;
ive two other methods used to prepare vaccines.
,illed microorganism;
modified toxin;
attenuated#heat treated#& treated microorganism;genetically engineered antigens;
isolated antigen;
Describe how the scientists could use 9och:s postulates to show
that the disease is caused by this bacterium.
0ho that bacterium is not present in any animal ithout thedisease;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
46/205
*mmunity
xplain why protein molecules are particularly well
suited to carry out the role of antibodies.
large variety of different molecules;
range of shapes;
5
tertiary shape;
loc,s onto # complements specific antigen;
xplain how the respiratory system stops pathogens
getting and the stomach reduces numbers
(a) mucus traps pathogens;
lyso@yme brea,s don bacterial cell alls;
cilia moves pathogens to pharynx;
here they are either salloed or removed;
max. !
(b)
en@yme;
in saliva or gastric >uice;
acid in stomach;
disrupts bacterial membrane # all;
xplain how a host is made less susceptible by the use of
&accination.
nature of vaccine e.g. attenuated strain;
vaccine introduces antigen;
stimulate # sensitise lymphocytes;
memory cells produced;if host meets pathogen (folloing vaccination);
production of same (8#T) lymphocytes;
large number # rapid production of plasma cells # antibodies #
T ,iller cells;
pathogen destroyed before it can affect host;
xplain how the defence mechanisms of the body
reduce the chance of entry by a pathogen.
Epidermis of s,in is dead # ,eratinised so pathogenscannot penetrate;
mucus in respiratory system is trapping stic,y
pathogens;
cilia move fluid # mucus removing pathogens;
tears # saliva # mucus contain lyso@yme brea,ing don
bacterial cell all;
stomach contains hydrochloric acid hich destroys
bacteria;
blood clot prevents entry;
fluid nature of tears ash aay bacteria;
vaginal acid destroys bacteria;
commensal bacteria on s,in compete ith pathogen;
sebum (fatty acid) inhibits bacterial groth;
%&'
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
47/205
*mmunity
xplain how the body responds both generally and specifically to
pathogens that enter the blood.
action of phagocytes;
ections +ualified e.g. ob>ections to use
of fetal #
animal tissue;
consider lo ris, of disease hen high percentage of
population already
vaccinated#5ef. to A4ead EffectB
xplain how &accination protects against de&eloping a
disease.
T lymphocytes # cells recogni@e antigen in vaccine;
T cells attach to antigens # destroy antigens;
8 lymphocytes # cells clone;roduce lasma ells
produce antibodies (hich ,ill microbe);
memory cells; rapidly produce of these antibodies on re
infection
*any elderly people are &accinated against influen!a.
xplain why it is necessary to &accinate these people
e&ery year.
influen@a virus mutates;
different strains # different shaped antigen;
mutant forms ill not be recognised by lymphocytesmemory cells
immune system; accept elderl1 have weaker immune s1stem
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
48/205
*mmunity
Suggest how cloning results in the production of 7-
lymphocytes that all ha&e the same antibody-producing
capability
Mitosis;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
49/205
*mmunity
%n antigen in a &accine leads to the production of
antibodies. Describe the part played by 7 lymphocytes in
this process.
1 macrophages present antigens to 8 lymphocytes;
2 antigen binds to#is complementary to receptors on
lymphocyte;
! binds to a specific lymphocyte;
$ lymphocytes become competent#sensitised;
% (8) lymphocytes reproduce by mitosis #(8) lymphocytes
cloned;
' plasma cells secrete antibodies;
+hen a pathogen enters the body it maybe destroyed by phagocytosis. &escribehow.
10 )hagoc*te attracted b* a substance& recognisesforeign antigen;20 )athogenengulfed& ingested;30 Enclosed in vacuole& vesicle& 'hagosome;!0 6acuole fuses&joins .ith l*sosome;$0 *sosome contains en9*mes;%0 )athogen digested& molecules h*drol*sed;
hagocytes and lysosomes are involved in destroying
microorganisms. -escribe ho.
hagocytes engulf pathogens#microorganisms;
Enclosed in a vacuole # vesicle# phagosome;
*ysosomes have en@ymes;
That digest#hydrolyse
molecules#proteins#lipids#microorganism;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
50/205
Unit #
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
51/205
3
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
52/205
3
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
53/205
3
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
54/205
Mitosis
Describe the beha&iour of chromosomes during mitosis
and explain how this results in the production of two
genetically identical cells. (
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
55/205
Mitosis
Describe the role of the spindle in mitosis.
"ttachment of centromeres;
0eparation of (daughter) chromatids;
Mitosis is important in the life of an
organism. ive two reasons why.
10 5ro.th & increase in cell number;20 =e'lace cells & re'air tissue & organs &bod*;30 5eneticall* identical cells;!0 Ase
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
56/205
Meiosis
Describe what happens to chromosomes in meiosis.(6)
1. hromosomes shorten#thic,en#condense;
2. hromosomes associate in homologous#(described) pairs #
formation of bivalents # tetrads;
!. rossingover # chiasma formation;
$. Poin to spindle (fibres) # moved by spindle;(K)
%. ("t) e:uator#middle of cell;(K)
'. (>oin via) centromere # ,inetochore;(K)
. (4omologous) chromosomes move to opposite poles #
chromosomes separate#move apart; (A""3; Aare pulled
apartB)
+. (airs of) chromatids separated in 2nddivision;
max '
(K) 5 C independent assortmentD
un:ualified L 1 mar,xplain how crossing o&er can contribute to genetic
&ariation.
sections of chromatids exchanged;
sections have different alleles;
ne combinations of (lin,ed) alleles;
*eiosis results in genetic &ariation in the gametes which
leads to &ariation in the offspring formed by sexual
reproduction. Describe how meiosis causes this &ariation
and explain the ad&antage of &ariation to the species.(5)
1. rossingover; ,$3&>any rong ref. to timingN
2.
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
57/205
Meiosis
Explain the importance of meiosis in the life cycle of a
sexually reproducing organism.
Meiosis halves the number of chromosomes;
5estoration of diploid number at fertilisation;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
58/205
Mitosis
ive twoprocesses hich occur during interphase and
hich are necessary for nuclear di&ision to takeplace.
replication of D/%0
% production0
synthesis of proteins=spindle=replication of
centrioles0
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
59/205
Meiosis
-uring meiosis7 one chromosome from each
homologous pair goes to each of the cellsproduced explain hy
to get haploid#n#half number of chromosomes (in
cells);
so that each cell gets one copy of eachchromosome#gene#full set of genes;
so that fertilisation produces diploid#constantchromosome number;
results in independent assortment;
xplain ho crossing over can contribute to geneticvariation.
sections of chromatids exchanged;
sections have different alleles;
ne combinations of (lin,ed) alleles;
"part from increasing genetic variation7 explain hy
meiosis is important in organisms hichreproduce sexually.
haploid cells produced#halves chromosome number;
fertilisation#fusion of gametes7 diploid number
restored;
chromosome number constant at each generation;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
60/205
Benetics*30ugar F phosphate bac,bone gives strength;oiling gives compact shape;0e:uence of bases allos information to be stored;
*ong molecule # coiling stores large amount of information;
omplementary base pairing enables information to be replicated #transcribed;
-ouble helix protects ea, hydrogen bonds # double helix ma,esmolecule stable;Many hydrogen bonds together give molecule stability;
revents code being corrupted;4ydrogen bonding allos chains to split for replication #transcription 5
molecule un@ips easily for replication # transcription.
Explain hy specific base pairing is important in -/"replication.
identical#exact copies made;
same base se:uence as original -/";
both strands act as template#complementary base
pairing
occurs on both strands;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
61/205
Aariation
8i&e the meaning and explain one possible cause of each
of the following types of &ariation. ontinuous &ariation
and discontinuous &ariation
5ange beteen extremes#no discrete types;
strong environmental influence;
polygenic#many genes involved;
:uantitative.2
discrete types;
little#no environmental influence#only genetic;
(often alleles of) 1#2 gene;
:ualitative.
3ithin each subspecies there is a range of phenotypes.
xplain the factors that gi&e rise to this &ariation. ()
phenotype depends on genotype and environment;
different local environments can produce variation;
different selection pressures;
mutations producing ne alleles;
meiosis produces ne combinations of alleles#example;
random fusion of gametes # sexual reproduction
Scientists, analsis of blood proteins has indicated alac! of genetic diversit in populations of someorganisms. Describe the processes that lead to areduction in the genetic diversit of populations oforganisms.
10 Mar# for general 'rinci'le of / reduced variet*&number ofdifferent alleles&,-A & reduced gene 'ool in ne.
'o'ulation;20 Counder effect;30 A fe. individuals from a 'o'ulation become isolated&formcolonies:!0 5enetic bottlenec#s;$0 Significant fall in si9e of 'o'ulation%0 Selective breeding & artificial selection;(0 Dsing organisms .ith 'articular
alleles&traits&'henot*'es&characteristics;
ive the meaning and explain onepossible cause of
each of the folloing types of variation
continuous and discontinuous.
(i) range beteen extremes#no
discrete types;
strong environmental influence;
polygenic#many genes involved;
:uantitative. 2
(ii) discrete types;
little#no environmental influence#only
genetic;
(often alleles of) 1#2 gene;
:ualitative.
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
62/205
%lood
essels
Describe how blood vessels are adapted for theirfunctionAll vessels have endothelium that reduces friction;rterThic#est .all enabling it to carr* blood at high 'ressure &.ithstand'ressure surges;most elastic tissue .hich smoothes out flo. & maintains'ressure;most muscle .hich maintains 'ressure;muscle in .all to control blood flo.;-apillarThin .all allo.ing diffusion&e
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
63/205
%lood essels
?hen the muscles contract7 the pressure
of the blood in the part of the veinbeteen valves %and 7 changes.
Explain ho this change in
pressure7 together ith the action of
the valves7 helps the blood to floto the heart
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
64/205
%lood essels
The diameter of a capillary is approximately the
same as the diameter of a red blood cell.Explain one ay in hich this increases
the efficiency of the exchange of
respiratory gases.
lose contact beteen cells and capillary alls;
5educes diffusion path;
or
0los passage of cells through capillary;
More time for diffusion#to reach e:uilibrium;
Explain ho blood capillaries are adapted for theirfunction of gas exchange.
large numbers #netor,7 so large surface area fordiffusion # gas exchange;
thin alls#one cell thic,7 so short diffusion distance;
(not +ust thin or thin membrane)
flattened cells in alls7 so short diffusion distance;
narro lumen7 so red cells touch alls#pass singly;
alls # membranes permeable % porous to gases for
diffusion'
(notAlots of poresB)
(accept low rate of flow' so more time for diffusion%gas
exchange)
(allow * for two features without explanation)
(re+ect fenestrated)
-escribe two ays by hich blood flo in the
veins is maintained.
valves prevent bac,flo;
residual blood pressure from heart;
effect of (s,eletal) muscle contraction
negative pressure from thorax;
Asuction effectB from heart;
ive twoays in hich the structure of a arteryis different from the structure of a vein.
Thic, muscular alls;
reater elastic content;
-o not have valves;
0mall#narro lumen;
54plain the di&erence in thickness between thepulmonar arter (thicker) and the pulmonar
;he thickness of the aorta wall changes all thetime during each cardiac c cle 5 plain h
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
65/205
%lood
vessels
pulmonary artery (thicker) and the pulmonaryein (thinner)!
igh 'ressure & smoothes out blood flo. & arter* .allcontains more collagen & muscle & elastic fibres &connective tissue;
time during each cardiac cycle! 54plain why!
10 Aorta .all stretches;20 8ecause ventricle&heart contracts & s*stole & 'ressureincreases;30 Aorta .all recoils;!0 8ecause ventricle rela
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
66/205
Classi-catio
n
Scientists studied two species of orth mericanseahorse. The thought that these two species areclosel related. Describe how comparisons of biologicalmolecules in these two species could be used to find outif the are closel related."om'are ,-A;Se>uence of bases&nucleotides;,-A h*bridisation;Se'arate ,-A strands & brea# h*drogen bonds;Mi< ,-A&strands of different s'ecies;Tem'erature&heat re>uired to se'arate h*brid strandsindicatesrelationshi';"om'are same&named 'rotein;Se>uence of amino acids &'rimar* structure;7mmunological evidence B not a mar#7nject seahorse 'rotein&serum into animal4btain antibodies&serum;Add 'rotein&serum&'lasma from other seahorse s'ecies;
Amount of 'reci'itate indicates relationshi'
"(plain the principles biologists use to classify
organisms into groups compared to older
models.
Consider phylogeny
@ook at eolutionary lineage*history
,ind the point of diergence from a common
ancestor
Consider= genetic= biochemical= embryology=
homology of anatomy
6rganisms are arranged in a hierarchy where large
ta4a (groups) are subdiided into smaller ta4a
(>= 2= C= 6= ,= B=7)0s groups get smaller the similarities between the
species increase
5ach species is gien a binomial name using the
genus and species
6lder models of classi-cation used obserable
features to group organisms
ow does a phlogenic sstem differ to a simple
hierarch$3 mauence is related to ,-A base&tri'letse>uence;
OR
!0 Similar s'ecies have a similar immune res'onse to a'rotein&named 'rotein;$0 More closel* related s'ecies 'roduce more@'reci'itate & antibod*/antigen com'le
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
68/205
64ygen
dissociation
Describe how haemoglobin is in&ol&ed in absorbing oxygen in
the lungs and transporting it to respiring tissues.
1. diffusion of oxygen into red cell # haemoglobin in red cells;2. high affinity of haemoglobin in high oxygen concentration;
!. (therefore) loads # becomes saturated in lungs # here oxygenabundant;$. oxyhaemoglobin formed;
%. reference to role of haem e.g. energy changes #role of 9e2I
ions # 4b molecule combines ith feer oxygen molecules;'. unloads # lo affinity in lo concentration;
. explanation in terms of dissociation curve i.e. small changesin concentration gives large changes in saturation;+. respiration in tissues gives high 2concentration # high
temperature # high 4I
concentration # lo p43. dissociation curve shifts to right # oxyhaemoglobin dissociationat higher partial pressure
"(plain how o(ygen is loaded, transportedand unloaded in the blood.-/
10 aemoglobin carries o
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
69/205
64ygen dissociation
During exercise" the rate of respiration of muscle cells
increases. xplain what causes human haemoglobin to
unload more oxygen to these cells.
artial pressure on oxygen in muscle falls more;
high # more carbon dioxide produced;
loers 4;
increase in temperature;
percentage saturation of 4b falls # loers affinity #
increase dissociation;
displaces curve to right # results in 8ohr shift;
Explain ho oxygen in a red blood cell is madeavailable for respiration in active tissues.
*o p4#(more)4I; due to (increased) 2
(increased) respiration;
(ignore refs to buffering action of haemoglobin)
(increased) dissociation of haemoglobin;
*o oxygen tension in tissues#plasma;
xygen diffuses from r.b.c. to tissues;
xplain ho the fetal haemoglobin ma,es it
possible for the fetus to ta,e oxygen fromthe motherBs blood.
9etal haemoglobin has greater affinity for#binds more
readily to oxygen;
at same pp2#concentration of oxygen7 fetal has highersaturation;
correct use of figures from graph (O and pp);
maintains diffusion gradient across placenta.
here is an ad&antage to the shrew in ha&inghaemoglobin with a dissociation cur&e
shifted to the right. xplain this
ad&antage.
(at the tissues at lo pp oxygen) the shreBshaemoglobin is less
saturated ith oxygen # has reduced affinity;
oxyhaemoglobin dissociates more readily #
haemoglobin releases
oxygen more readily # more oxygen released;
alloing greater demand # respiration rate;
The blood leaving a muscle has a loer p4 than the -escribe and explain ho an increase in the rate of
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
70/205
64ygen dissociation
The blood leaving a muscle has a loer p4 than the
blood entering it. -uring vigorousexercise7 the fall in p4 is even greater.
Explain hat causes this greater fall in
p4.
(in exercise) fasterrespiration rate;
more 2production;
2is acidic # forms carbonic acid;
lactic acid production;
release of 4Iions;
Explain the advantage to the lugorm of having
the dissociation curve to the right giventhat it lives in an area ith lo oxygen
levels.
"ble to be saturated ith oxygen in (very) lo
concentration;
related to lo oxygen concentration inenvironment;
able to unload at only slightly loer
concentration;
-escribe and explain ho an increase in the rate of
respiration in the tissues of a mammalaffects the oxyhaemoglobin dissociation
curve.
(more) carbon dioxide;
decrease in p4#increased acidity#4 ions;
curve moves to the right#depressed;
more oxygen released#4 ions combine ith 4b#4b
reduced;
arbon dioxide helps haemoglobin to
release oxygen to rapidly respiringtissues.
se the graph to explain ho.
-isplaces
dissociation curve to the right#8ohrshift;
*oer affinity for oxygen#less saturated
ith oxygen;
0uggest the advantage to a
d i l f h i h l bi
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
71/205
64ygen dissociation
ground s:uirrel of having haemoglobin
that has an oxygen dissociation curve to
the left of the curve for humanhaemoglobin.
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
72/205
;issue ,luid
"(plain how tissue 0uid is formed and how
it may be returned to the circulatory
system.
(4ydrostatic) pressure of blood high at arterial end;
2. 9luid#ater#soluble molecules pass out (re+ect plasma);
!. roteins#large molecules remain;$. This loers the ater potential # ater potential becomesmore negative;
%. ?ater moves bac, into venous end of capillary (re+ect
tissue fluid);
'. 8y osmosis # diffusion;. *ymph system collects any excess tissue fluid;
+. (*ymph) returns to blood # circulatory system # lin, ith
vena cava#
returns tissue fluid to vein;
The tissues of people ho are starving often
sell because of the accumulation oftissue fluid. Explain hat causes this
accumulation of tissue fluid.
0tarvation lin,ed to lo protein content of
diet#*o protein concentration
in plasma#blood;
?ater potential of blood higher#smaller ater
potential gradient;
Tissue fluid formed faster than returned#less
tissue fluid returned to blood;
-escribe the part played by proteins in theplasma in returning tissue fluid to the
capillary.
roduces loer ater potential;
?ater moves into capillary;
8y osmosis#diffusion;
-escribe and explain one ay in hich the
composition of tissue fluid differs from
that of plasma.
contains little#no protein;
molecules too large (to pass through capillary
all);
or
contains less glucose;
some ill have entered tissue cells;
2 max
accept an1 other biologicall1 correct difference
marked in a
similar wa1!
-escribe ho tissue fluid is reabsorbed into 4istamine increases the permeability of
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
73/205
;issue :uid
-escribe ho tissue fluid is reabsorbed into
blood capillaries.
eneral principle=
blood exerts an Cosmotic forceD hich causesfluid to move bac,
into capillaries;
-etail=
caused by plasma proteins (retained in blood);
ater moves into blood by osmosis# diffusion;
small soluble molecules move into blood by
diffusion;
Explain the lin, beteen insufficient protein in
the diet and the accumulation of tissue
fluid.
*ess protein in blood # plasma # capillary;
?ater potential of blood increases;
*ess reabsorption occurs # lymph system cannotdrain excess.
4istamine increases the permeability of
capillary alls so that large molecules canpass through. Explain ho this change in
permeability results in selling round thebite.
roteins can move into tissue fluid;
*oers ater potential of tissue fluid;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
74/205
'issue 0uid
gg p g
blood pressure and theaccumulation of tissue fluid.
4igh blood pressure increases rate of filtration #
forces more fluid out;
*ymph system cannot cope # higher pressure
reduces reabsorption;
3escribe how tissue :uid is formed and how it is
returned to the circulatory system!
Cormation10 igh blood & h*drostatic 'ressure & 'ressure filtration;20 Corces .ater & fluid out;30 arge 'roteins remain in ca'illar*;
=eturn!0 o.er .ater 'otential in ca'illar* & blood;$0 ,ue to 'lasma 'roteins;%0 Water enters ca'illar* & blood;
(0 8* osmosis;+0 "orrect reference to l*m'h;
p
ater is exchanged beteen the blood andtissue fluid as blood flos along the
capillary.
4 forces ater out;
idea that 4 is ChigherD than ?;
proteins remain in blood (increases ?);idea that ? is no ChigherD than 4;
ater returns by osmosis # along ?
gradient;
ater moves out at arteriole end and bac,in (at venule end);
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
75/205
Bas
e4change
his insect has more than >.5 million tracheoles. he
distance between the ends of the tracheoles in the muscle
is approximately pm. xplain how these features allow
efficient oxygen supply.
@arge number gies large (total) surface area"
,or di&usion"
7hort distance between tracheoles gies shortpathway"
Moement*di&usion through muscle is slow"
Reject references to muscle simply being close
to tracheoles. Must convey
idea of short pathway to gain credit for third
point.
% thin surface and a diffusion gradient are both featuresof gas exchange surfaces. Describe how these are achie&ed
at the gas exchange surfaces of a mammal0
(8all of) aleoli * capillaries hae single epithelial
layer*
single layer of cells*
aleoli and capillaries close together"
epithelium :attened*paement epithelium"
entilation maintains high 6#*low C6#
concentration(in aleoli)"
blood :ow*circulation maintains high C6#*
low 6#concentration(in blood)"
xplain how the features of the al&eoli maximise
absorption of oxygen into the blood.
@arge surface area"
:attened cells * suamous epithelium * single
layer of epithelial cells"
short di&usion pathway"
role of surfactant"
e4tensie blood supply maintains a high di&usion
gradient"
narrow capillaries slows blood :ow D more time
for di&usion"
xplain how the gills of a fish are adapted to form aspecialised exchange surface.
0tructure of filaments;
large number of lamellae;
9lattened epithelial cells;0hort distance beteen ater and blood # short diffusion
pathay # maximum diffusion gradient;
ountercurrent mechanism # description;
maintains diffusion gradient along length # prevents oxygenconcentrations reaching e:uilibrium;
Role of entilation mechanism in producing water
:ow oer gills"
Circulation replacing blood"
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
76/205
Bas
e4change
&escribe the counter current principle of
gas e(change in 1sh
counter current* blood :ow in opposite direction
to water :ow"
giing ma4imum distance for o4ygen to :ow
in*carbon dio4ide out*gas e4change"
description of how a counter current works"
gill lamellae gie a large surface area"
thin surface of lamellae*short distance between
surface of lamellae and blood capillaries"
lamellae held edge on to water :ow"
:ow of water across gills in one direction"
entilation system to maintain the water :ow"
blood circulation to maintain the blood :ow"
n insect lives in air. Describe how the insect isable to obtain oxgen and limit water loss.
1 Air enters through o'en s'iracles;2 Through tracheae;3 ,iffusion gradient in trachea! Tracheae associated .ith all cells&closel* associated.ith cells;$ 4
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
77/205
Bas
e4change
mammalian breathing system enables efficient uptake of
oxygen into the blood.(6)
1. alveoli provide a large surface area;
2. alls of alveoli thin to provide a short diffusion pathay;
!. alls of capillary thin#close to alveoli provides a short diffusion pathay;
$. alls (of capillaries#alveoli) have flattened cells;
%. cell membrane permeable to gases;
'. many blood capillaries provide a large surface area;. intercostal#chest muscles#diaphragm muscles # to ventilate
lungs # maintain a diffusion#concentration gradient;
+. ide trachea # branching of bronchi#bronchioles for
efficient flo of air;3. cartilage rings ,eep airays open;
-escribe ho a large difference in oxygen
concentration is maintained beteen a fish gill and thesurrounding ater.
9ish has ventilation system hich replaces ater;
highly oxygenated ater
(circulatory system brings in) blood ith lo
concentration of
oxygen#blood removes oxygen;
counter current system#description;
surfaces. -escribe ho these are achieved at the gas exchange surfaces of
a mammal and leaf
(all of)alveoli # capillaries have single epithelial layer#
single layer of cells#
alveoli and capillaries close together;
epithelium flattened#pavement epithelium;
ventilation maintains high 2#lo 2concentration(in alveoli);
blood flo#circulation maintains high 2#
lo 2concentration(in blood);
max. !
(ii) leaf very thin # only a fe cells thic,;
intercellular spaces exposes cell surface membrane#
all directly to gases;
production of 2in photosynthesis maintains high oxygen concentration;
use of 2in photosynthesis maintains
lo carbon dioxide concentration;-escribe the adaptations of a mammalian lung hich
ensure a short diffusion pathay for respiratory gases.
ne cell thic,#single layer of(epithelial) cells lining
alveolus;
flattened#pavement#s:uamous;
capillaries surrounded by single layer of cells;
capillaries and alveoli are close;
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
78/205
as
e(change
This insect has more than 1.% million tracheoles. The
distance beteen the ends of the tracheoles in the
muscle is approximately $ pm. Explain ho these
features allo efficient oxygen supply.
*arge number gives large (total) surface area;
9or diffusion;
0hort distance beteen tracheoles gives short pathay;
Movement#diffusion through muscle is slo;
acent cells # alls not touching.
uggest tworeasons hy it ould be very difficult toextract sufficient oxygen from ater by moving ater
in and out of lungs.
?ater too dense to move in and out of lungs;
lo oxygen concentration in ater;
high metabolic rate in mammals re:uires high oxygen
inta,e.
Explain ho the countercurrent principle helps fish to
extract oxygen from ater.
Explain ho the ventilation mechanism of a fish and
the structure of its gills result in the efficient upta,e of
oxygen from ater
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
79/205
as
e(change
?ater flos in opposite direction to blood;
across (gill) lamellae;
so difference in concentration maintained;
diffusion gradient maintained # diffusion over full
length.
-escribe and explain ho fish maintain a flo ofater over their gills.
1. mouth opens7 operculum#opercular valve shuts;
2. floor of mouth loered;
!. ater enters due to decreased pressure # increased
volume;
$. mouth closes7 operculum#opercular valve opens;
%. floor raised results in increased pressure # decreasedvolume;
'. high#increased pressure forces#pushes ater overgills;
oxygen from ater.
-escribe and explain ho the structure of the mammalianbreathing system enables efficient upta,e of oxygen into the blood.
1. alveoli provide a large surface area;
2. alls of alveoli thin to provide a short diffusion pathay;
!. alls of capillary thin#close to alveoli provides
a short diffusion pathay;
$. alls (of capillaries#alveoli) have flattened cells;
%. cell membrane permeable to gases;
'. many blood capillaries provide a large surface area;
. intercostal#chest muscles#diaphragm muscles # to ventilatelungs #
maintain a diffusion#concentration gradient;
+. ide trachea # branching of bronchi#bronchioles for efficient
flo of air;
3. cartilage rings ,eep airays open;
-escribe ho the gills of a fish are ventilated after
ater has entered through its mouth.
"n insect lives in air. -escribe ho the insect is able to
obtain oxygen and limit ater loss.
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
80/205
as e(change
g
fish closes mouth and raises the floor of the mouth;this decreases the volume # increases the pressure (of
mouth);
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
81/205
as e(change
p p
flo of ater and of blood shon in the micrographis useful to a fish.
Maintains concentration gradient (over hole length
of gill) # diffusion can
occur over hole gill;
More oxygen enters blood (# more 2leaves);
More (aerobic) respiration # more energy release in
muscle # for simming;
more needed 3$> onl1
0ir moes into the lungs during inspiration!54plain how the diaphragm causes this!
10 ,ia'hragm contracts&moves do.n& flattens;20 7ncreases volume of thora
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
82/205
0ntibiotics
"(plain how resistance to an antibiotic could
become widespread in a bacterial population
following a gene mutation conferring resistance
in just one bacterium.
1! freuent use of antibiotic creates selection
pressure* antibiotic kills bacteria"
#! bacteria with natural mutation* resistance hae
(selectie) adantage oer others * described"
$! (surie to) reproduce more than other types"
! pass on adantageous allele* mutated allele in
greater numbers"
+! freuency of (adantageous) allele increases in
subseuent generations"
(penalise use of gene! instead of allele once only"
.! freuency of resistant types increases in
subseuent generations"
ive two other ays in hich antibiotics canprevent bacterial groth.
disrupts cell all#prevents cell all synthesis;
stops -/" replication;
Describe one other wa in which antibiotics can act againstbacteria. Explain wh this mode of action is effective againstthe bacteria.)revent ,-A re'lication & 'revent m/=-A s*nthesis & 'reventtransfer of amino acids to ribosomes & re'roduction & transcri'tion &translation & 'rotein s*nthesis;
1
)reventing ,-A re'lication: bacterial cell .ill be unable to divide;
)revent re'roduction 2nd
'oint onl*; 'o'ulation of bacteria .ill not
increase;/0
)reventing m/=-A s*nthesis & no m/=-A means code not 'assed
to
transcri'tion; ribosomes;no 'rotein s*nthesis & no ne. en9*mes;
/0
)reventing transfer of amino acid no 'roteins made;
to ribosomes & translation no en9*mes & no 'roteins structures;
'revent 'rotein s*nthesis 2nd
'oint onl*;
3escribe how gene transmission and selectionhae increased the diEculty of treating bacterialinfections with antibiotics!
10 Antibiotic resistant gene&allele;20 6ertical gene transmission;30 =esistant bacteria survive and re'roduce &'o'ulation of resistant bacteria increases;!0 7ncrease in fre>uenc* of resistant allele&gene infuture generations;$0 ori9ontal gene transmission;0 )lasmid;(0 "onjugation & 'ilus tube;+0 ori9ontal transmission& conjugation can occurbet.een bacteria of different s'ecies;
Describe the structure of a cellulose molecule and explain
&escribe how the structure of (ylem is
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
83/205
7tructures
and function
Describe the structure of a cellulose molecule and explain
how cellulose is adapted for its function in cells.
Made from FDglucose"#! Goined by condensation*remoing molecule ofwater*glycosidic bond"$! 1H link speci-ed or described"! I,lipping oerJ of alternate molecules"+! 'ydrogen bonds linking chains*long straight
chains".! Cellulose makes cell walls strong*cellulose-bres are strong"/! Can resist turgor pressure*osmoticpressure*pulling forces"! %ond diEcult to break"?! resists digestion*action ofmicroorganisms*enzymes"
Palisade cells are the main site ofphotosynthesis. "(plain one way in which a
palisade cell is adapted for photosynthesis.
idea ofmany chloroplasts * lots of chlorophyll"
to trap or absorb light (energy)"
elongated cells with long a4is perpendicular to the
surface"
idea o#ight has a longer pathway allowing ma4imum
light absorption * light penetration"
chloroplasts moe"
to trap or absorb light (energy)"
range of pigments"can absorb a range of waelengths * colours * for ma4
light absorption"
large 7!0! or cell wall feature e!g! thin * permeable"
for (rapid) C6#absorption"
&escribe how the structure of (ylem is
related to its function.
Aessels"
'ae no end walls * hollow * no cytoplasm"
0llows unrestricted :ow of water!
@igni-cation"
2roides support * strength * impermeability"
2its allow lateral transport"
;racheids with porous end walls!
"(plain how the properties of starch arerelated to its role in living organisms -2/
role storage;plus
properties insoluble;
explanation therefore stays inside cell#membrane;
orproperties large molecule#coiled#branched;
explanation lots of glucose#carbohydrate molecules in small
space#stays inside cell;
or
properties osmotically inactive;explanation does not cause the cell to absorb ater;
Explain ho cellulose gives cotton its strength. 'wo ways in which the structure of cellulose is
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
84/205
Structures for
function
(long) straight#unbranched chains;
(idea of more than 1) chains lie side by side #
form (micro)fibrils;
idea of 4 bonds holding chains together;
"(plain one way in which the structure of
cellulose is linked to its function.
10 /bonds & micro¯o fibrils &fibres;
20 Strength & rigidit* & inelasticit*;
di&erentfrom the structure of starch!
starch10 1! and 1% bonds&contains 1% bonds &branching20 All glucoses& monomers same .a* u'30 eli
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
85/205
2lant
transport
of a tree to the lea&es
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
86/205
2lant
transport
7n da*light most of the .ater eva'orates from the leaves butsome is used b* the 'lant0,escribe the .a*s in .hich this .ater could be used b* the'lant0 %.hich is in a continuous column & .ater molecules cohere;cohesion due to bonding;column doesnt brea# because of adhesion .ith
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
87/205
2lant
transport
stem blocks the mo&ement of water through that &essel.
Bse the cohesion-tension theory to explain why.
Evaporation from leaves # transpiration;
?ater in xylem under tensionK#negative pressure#pulled up;
?ater molecules cohereK#stic, together#form hydrogen
bonds;,gnore: references to adhesionN
0o ater a single column;"ir bubble brea,s column # prevents cohesion;
3se our !nowledge of the cohesion4tension theor of
water movement through a plant5 to explain wh thediameter of the trun! is smallest at midda.10 ,iameter of trun# minimal at .armest & brightest time of da*&midda* F .armest & brightest;20 Stomata o'en in light more .ater loss;30 Water eva'orates more .hen .arm & more heat energ* for.ater eva'oration;!0 *drogen/bonding bet.een .ater molecules;$0 "ohesion & described bet.een .ater molecules;%0 Adhesion described bet.een .ater molecules and .alls of
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
88/205
2lant transport
"poplastic F &ia cell alls # spaces external to cell membrane # external to
cytoplasm # beteen cells;
"s far as endodermis # asparian strip # layer of ax;
aused by transpiration pull;
ohesion # hydrogenbonding beteen ater molecules;
0ymplastic F Through cell surface membrane (of epidermis # root hair cell) #
ref.vacuoles membrane;
4igh to lo # s;
-iffusion # osmosis;
elltocell via plasmodesmata # via strands of cytoplasm;
0ecretion # active transport of ions into xylem by endodermis;
5
"ctive upta,e of ions from soil at epidemis;
*oers # sin xylem # increases osmosis into xylem;Explain ho root pressure and cohesionFtension are responsible for the
movement of ater in xylem vessels.
5oot pressure=
1. "ctive transport of salts into xylem;
2. Endodermis # asparian strip;
!.revents lea,age # ater # ions must use symplast pathay;
$. *oer ater potential inside xylem;
%. ?ater (enters xylem) don ? gradient # by osmosis;
'. pard ater movement by root pressure is relatively lo;
$ max
ohesion tension=
. Transpiration # evaporation of ater;
+. 9rom spongy mesophyll # through stomata;
3. *oers ater potential of mesophyll;
16. ?ater molecules hydrogen bond # stic, together;11. 5ef. to columns # chains;
12. ?ater pulled up xylem (creating tension);
1!. "dhesion beteen ater molecules and xylem vessel alls;
1$. 5esponsible for ma>ority of ater movement up xylem vessels;
is smallest at midday.
1. -iameter of trun, minimal at armest # brightest time of day #
midday L armest # brightest;
2. 0tomata open in light more ater loss;
!. ?ater evaporates more hen arm # more heat energy for ater
evaporation;
$. 4ydrogenbonding beteen ater molecules;
%. ohesion (# described) beteen ater molecules;
'. "dhesion (described) beteen ater molecules and alls of
xylem vessels;
. (Jylem) pulled inards by faster flo of ater # pulled in by
tension;
+. 5educed pressure at leaves # top of plant # pull from top # from
leaves #
tension from leaves # from top of plant due to transpiration #
evaporation;
3. ?ater pulled up plant;Explain ho the negative pressure in the xylem
vessels of the leaves causes ater to move
up the plant from the xylem in the roots.
1. continuous # leaf to rootcolumn of ater;
2. 4bonds;
!. cohesion;
$. column under tension # pull
transmitted;
-escribe onepiece of evidence that supports the
root pressure theory and explain ho it
supports this theory.
The diameter of a tree is less during the day7 hen the tree is
transpiring7 than it is at night. Explain ho this supports
cohesion but not root pressure
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
89/205
2lant transport
0uitable accepted evidence7 1 mar, for evidence and 1
mar, for explanation F
E
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
90/205
2lant transport
an increase in air temperature anddecrease in ater content of soil
(i) 4igher temperatureprovides more ,inetic energy;
9or evaporation # diffusion;
"ir can hold more ater vapour # increases
ater potential gradient;2 max
(ii) 5educestranspiration as less ater upta,e;
5eference to ater potential gradient (leaf
and air # soil and root);
-escribe and explain ho ater in the
mesophyll cells passes out of theleaf.
(pathay from cells) along cell alls #
through spaces and out through stoma(ta);
by diffusion (disualif1 if osmosis
mentioned);
don a ?#diffusion#concentrationgradient;
according to the cohesion tensionhypothesis.
1. ater evaporates#transpires from leaves;
2. reduces ater potential in cell #aterpotential#osmotic gradient across
cells (ignore reference to air space);
!. ater is dran out of xylem;
$. creates tension (accept negative pressure not
reduced pressure);
%. cohesive forces beteen ater molecules;
'. ater pulled up as a column;
se your ,noledge of the cohesiontension
theory to explain ho ater in the xylemin the roots moves up the stem
ater evaporates#transpires;
reduces ater potential # creates ater potential
gradient # increases
osmotic gradient #
moves via apoplast pathay;
ater dran out of xylem;
creates tension#pulling effect # creates negative
pressure (in context)'
cohesive forces or 4 bonding beteen ater
molecules # ater moves
as a column;
-escribe two features you ould expect in the leaves of a tree
adapted to a dry environment. Explain ho each feature helps
xplain how the presence of Chairs and rolled lea&es
reduce water loss in xerophytic plants. ()
7/21/2019 Long Answer Questions A LEVEL BIOLOGY
91/205
Kerophytic
adaptations
adapted to a dry environment. Explain ho each feature helpsthe treeBs survival. (')
0un,en stomata;
ater evaporation into pit creates local humidity;
increased humidity reduces gradient for ater evaporation;
close arrangement of stomata;
diffusion shells of individual stomata overlap;
interferes ith ater diffusion and slos evaporation;
restriction of stomata to loer side of leaf;
rate of air movement belo leaf less# heating effect of sun
less;
gradient for ater evaporation reduced# ater molecules have
less
,inetic energy;
continued
thick cuticle*wa4*suberin (on upper surface)"(wa4*suberin )waterproof"
water unable to di&use onto surface to
eaporate=
presence of trichomes* hairs"
surface traps water close to leaf surface"
increased humidity reduces gradient for water
eaporation"
reduced leaes*spines*small surface area to
olume"
less surface area for eaporation"more distance across leaf for water to di&use"
rolled leaves;
stomata enclosed in localised humidity;
increased humidity reduces gradient for ater evaporation;
reduce water loss in xerophytic plants. ()
Trap moist air # increase humidity;
5educe air flo (around leaf surface # stomata);
*oer ? # ater vapour concentration gradient (beteen
inside
and outside of leaf);
0hield stomata from high temperature # high light
intensity # ind; ignore sun
5educe transpiration # evaporation # diffusion of ater(vapour);
-escribe and explain ho three structural features reduce the rate
of transpiration in xerophytic plants75olled leaves Freduces ater potential gradient air movement across stomata #traps air
hich becomes saturated # moist # humid # reduces surface area;0un,en stomata F
reduces ater potential gradient air movement across stomata #traps airhich becomes saturated # moist # humid;Thic, cuticle F
5educes cuticular transpiration # reduces ration greater diffusiondistance;
4airs
traps air hich becomes saturated # moist # humid;5educed leaves # spines Fless surface area # feer stomata (for evaporation).
3ater &apour diffuses through open stomata into the
atmosphere. Describe two structural adaptations of the lea&am