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LOGOEffects of a begomovirus on tritrophic
interaction of tomato, whitefly Bemisia tabaci and its parasitoid Eretmocerus hayati
SIP15 -2014
Yin-Quan Liu
Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China
Insect vector
?Plant Virus
Background Information
Turnip yellow Virus
Background Information
Higher larvae mortality
Longer developmental times
Calvo and Fereres, 2011. Biocontrol, 56(5): 747-757
Eretmocerus hayati
Solanum lycopersicumcv. Hezuo 903
Tomato Yellow leaf curl virus
(TYLCV)
Study System---Material
Bemisia tabaci
1.Olfactory Responses of whitefly and parasitoid to tomato plant ( Y tube experiments )
2.Foraging behavior of wasp E. hayati ( Observer system : Patch residence time and other parameters)
3.Ecological characters of wasps developed in whiteflies on virus-infected tomato or uninfected tomato plant
4.Comparison of leaf trichome density
Study System---Experiment design
主要结果主要结果
H : Uninfected tomato ; V : Virus-infected tomato ; HB : Whitefly-infected tomato ; VB : Co-infected tomato plant ; CK : Control
Main Results: Olfactory response
Olfactory response of whitefly to tomato plant
N(n)
主要结果主要结果
H : Uninfected tomato ; V : Virus-infected tomato ; HB : Whitefly-infected tomato ; VB : Co-infected tomato plant ; CK : Control
Main Results: Olfactory response
Olfactory response of parasitoid to tomato plant
N(n)
主要结果主要结果Main Results: Olfactory response
Main terpenoids detected in both virus-infected tomato and uninfected tomato (GC-MS)
1.beta.-Myrcene 2.alpha.-Pinene3.alpha.-
Phellandrene4.4-Carene5.alpha.-
Caryophyllene
Comparison of terpenoids in quantity is ongoing
主要结果主要结果Main Results : Foraging behavior
Treatment No. of patches tested
Patch residence
time (s)
Host handling time (s) Searching time per patch (s)
parasitism rate of
whiteflies (%)
The total handling time
per patch
Time for parasitizing per patch
Time for parasiting
a host
Feeding time on a host
Uninfected tomato 16 1509.0±191 564.6±92 245.2±37 72. 5±6 497.8±29 944.4±136 33.1±5
Virus infected tomato
16 2274.9±258 1106.0±179 406.1±62 89.6±9 1132.1±47 1168.8±131 43.8±6
P Values 0.024 0.012 0.034 0.12 0.008 0.88 0.20
Foraging behaviour of Eretmocerus hayati on tomato leaflets (Patches)
Each Patch contains 10 whitefly hosts.
主要结果主要结果Main Results : Parasitism rates
Uninfected Plant Virus-infected Plant
Parasitism rate ( Mean ± SE ) of Eretmocerus hayati on Bemisia tabaci reared on uninfected and TYLCV-infected tomato plants
主要结果主要结果Main Results : Emergence rates
Uninfected Plant Virus-infected Plant
Emergence rate ( Mean ± SE ) of Eretmocerus hayati on Bemisia tabaci reared on uninfected and TYLCV-infected tomato plants
主要结果主要结果Main Results : Developmental duration
Uninfected Plant Virus-infected Plant
Developmental time of Eretmocerus hayati on Bemisia tabaci reared on uninfected and TYLCV-infected tomato plants
主要结果主要结果
Uninfected Plant Virus-infected Plant
0
10
20
30
40
50
60
健康番茄 带毒番茄
每平
方毫
米叶
毛数
量
Num
ber
of
leaf
tric
ho
mes/
㎜
2
Virus-infected plantsUninfected plants
***
Uninfected Plant Virus-infected Plant
The leaf trichomes density of uninfected and TYLCV-infected tomato plants
Main Results : Leaf trichomes density
1.TYLCV showed asymmetrical effects to whitefly and wasp in their olfactory responses
2.The wasp preferred the infected plant when tomato was infected by either whitefly or virus
3.The wasp showed no preference between co-infected plants and whitefly-infected plant but prefer to co-infected plants in comparison with virus-infected plant (which implies whitefly infection had stronger effects than virus infection to wasps)
Conclusion and Discussion
4. Parasitoid had longer patch residence time in virus-infected tomato plants, and longer host-handling time.
5. Change of leaf surface feature including leaf trichomes density could be main factors that contribute to the difference.
6. The parasitsm rates, emergence rates and developmental durations of the parasitoid on infected and uninfected plants were similar, (similar suitability of whitefly--- Liu et al., 2009)
Conclusion and Discussion
LOGOAcknowledgements:Acknowledgements:
National Natural Science Foundation of China (31071736)National Basic Research Program of China (2013CB127604)
Liu, Shu-Sheng Li, Yuan-Xi
Pan, deng Yan, Ting-Ting
Zhang, Zhen