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Presented by Sonia Sharma BNFO 301. Locating the Repressor Binding Sites in the Bacteriophages. Repressors What are They. Bacteriophages when infecting the Bacteria have to make an important decision. If Life is bestowed. Lytic promoters have to be silenced - PowerPoint PPT Presentation
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Locating the Repressor Binding Sites in the
Bacteriophages
• Presented by Sonia Sharma
• BNFO 301
CONFIDENTIAL2
Repressors What are They
Bacteriophages when infecting the Bacteria have to make an important
decision
3CONFIDENTIAL
If Life is bestowed
• Lytic promoters have to be silenced• Repressors come to limelight to do
their job• They Block the promoter’s sites for
lytic pathway then proceed to Lysogeny
• Repressor also binds to DNA at specific sequences
• Found multiple times in genome
CONFIDENTIAL4
Objective is to locate those sites
• By Utilizing• computational tools integrated in BioBIKE,-• A graphical programming interface that enables
biologist to approach complex research questions • Upstream sequences of relevant phages
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Data- available for Che12, e-lambda,L5
and Bxb1
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Same method for L5 and Bxb1
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Testing Time• Apply the same technique to find to
binding sites of some random phages
• Packman• Shaka
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Concluding thoughtResults yet to be
analyzed