: : .: .. . lizard_Bassoon MGNEASLEGGGGEGQPQQQQLPHGPAAGPSAPPGSAKPPPSAPGGGGQLPAGRA--PAAVPAPGPALPHGPSARWEASGEPGREAERG--------------SVRQGGIEGEGRARGSPSTPRRPDQSDPSFGQRAASPSLKQASQT---SESTREIRSHGQ-TPQQADGPRRTLQVDSR---DQRPGRSPSASPDRGST 177mouse_Bassoon MGNEASLEGGAGEGPLP-------PGGSGLGPGPGAGKPPSALAGGGQLPVAGAARAAGPPTPGL------------GPVPGPGPGPG-----------------------------PGSVPRRLDPKEPLGSQRTTSPTPKQASATAPGRESPRETRAQGP-SGQEAESPRRTLQVDSR---TQRSGRSPSVSPDRGST 148coelacanth_Bassoon MGNEASLEGG--------------EGGQALQQGSSGAGSVSAPSTVGQLKAAS---SPSAAANGA------------AAGPGTPTHRG---------------------------------STRPNQLDQLAGQKAASQPFKQVGHSNESTKETQNHMQSGQRRQGSA----RTLHIDTS---SGKSGRSPSVSPDRSSA 131frog_Bassoon MGQ---------------------------LPGPSSAGASPAGTRHGE-----------ASASGR------------LENPGRLAGQS--------------------------------------------------SAPQSFRQTTSSTESTKDMKSHASPSHQQADSSRRTLQVESK---GSRTGRSPAVSPDRGTT 97zebrafish_bassoon_a MGNEASLEAG--------------DGAT------MAGMSVPAPPGSGQLLKP----VNGAAAGAGASAEHRAGMNRLAGAGGPPTSPK-------------LGYGAGSDRGPGADFGTGGMQTSANPYKKQIHDGPRHSQTTPTHQSQIQASAGGMGANQSP--GQHA--SRRNLQVDFS--SSSRTGRSPSASPDRTSA 157zebrafish_bassoon_b MGNEASLEGEGQPGQ---------PGGA--VPAAGAPASISAPAGGGQLHKP----SNGAPAGGM------------GTGHGPGMN---------------------------------SIQGKPNQTDPSMGPKPGGPPGVGPGPGAPTH-------PQGP-SQSQA--ARKNLQVDVG---GSKGG---PVSPG-SSA 123stickleback_bassoon_a MGNEASMEGGGQPGD---------PGAA-GMMGPVMPGGPATAPGAGQHMKP----VNGIAAGGG------------MGIGGPGMGMS--------------------------------------------------AVGHGGHPSP----------------GQHA--SRRNLQVDFSGSGGSRRGRSPSGSPDRGVT 106stickleback_bassoon_b MGNEASMEGEGQAGQ---------PGAA--VPPAGAPASISAPPDSGQLIKP----SNGAPAGGS------------GAGPGPGINSGRLIVSAGTQMSSVLTTERRKSPTAIPLCVLFIVSARPPPSDP--GPKAGVQSGHGTGDRLASHETRQHAAPQAE--QGHV--SRKSLHVDVG---SSRTGRSPSVSPDRGSV 164fugu_bassoon_a MGNEASMEGGGQPGE---------PGAAAGMMGSMMPGGPTPGAGAGQHMKP----INGAAAGGG------------MGVGGPAMGMT-RYGQCGDECGRVTSSGAGEPYRLATHESPVSPRHMQSSQASGMGQGPGSGSGHGMGLGPPGSSMGGHGHGGSP--GQHA--SRRNLQVDFS---GSRSGRSPSASPDRGVT 167fugu_bassoon_b MGNEASMEGEGQAGQ---------PGTA--VPATGAPASISAPPDAGQLIKP----SNGAPAGGS------------GAGPGPGIN-------------------------------------RPPLSDP--GPKAGMQSGHGTGDRLASHDTYQ---PQGEQGQGHV--SRKSLQVDVG---GSRPGRSPSVSPDRGSV 126medaka_bassoon_b MGNEASMEGEGQAGQ---------PGPA--VPAAGAPTSISAPPGSGQLLKP----SNGAPAGGS------------GAAPRPGIN-------------------------------------RPPPSDP----KPGGQSGHGTGDRLASHDASQHAAPQGTQGEGHA--ARRNLQVDLG---SNRSGRSPSVSPDRGNV 127medaka_bassoon_a MGNEASMEGGGE------------PGAA-GMMGSVMPGGPGAGPGAGQHTKP----VNGAAAGGG------------MGMTGPGMGMT----------------RAGEPYRLASHESPSSPRHMQTSQGASMGHGQG--TGHGMGQSSAMSVMGAHT---GQSQGQHA--SRRNLQVDFSGGGGSRTGRSPSVSPDRGMT 148tilapia_bassoon_b MGNEASMEGEGQAGQ---------PGPA--VPAAGAPASISAPPDSGQLIKP----SNGAPAGGR---------------HRSNVHLV---------------------------LCSLYLSVRPPPSDS--GPKAGGQIGHGTGDRLASHDTPQHAAPHGEQGQGHV--ARKSLQVDVG---SSRTGRSPSASPDRGSV 136tilapia_bassoon_a MGNEASMEGGGQPGE---------PGSA-GMMGSMMPGGPSQGP--GQHMKP----VNGATAGGG------------MGMGGPGMGMT---------------------------------RHMQSSQASGMGQGPG--SGQGMGHGPAGSSMGGHG---GQSPGQHA--SRRNLQVDFSGSGGSRTGRSPSLSPDRGVT 132cod_bassoon_a VQEWLCLNCQMQRA----------LGIDMTTPRSKSQQQIHSPSHQAKPIAQ----AQPLPPAAQ------------SKAPAQS-----------------------------------QPPHQPPATHP-----------YGQAQPYSQAPSPHHSQPYAQAQSQHH-------------------SQAYSQSQPHPQG 109cod_bassoon_b MGNEGSVEGEGPAVQ---------GGPA--APAAGAPASVSAPPAPGQLTKP----SNGAPAGGT------------GAGPGPGINR----------------------------------------------------------------------------------------------------------------- 60

*** .* * ..: * . . : *. *. .:*:*: .:: * . * **.:: . . lizard_Bassoon PTSPYSVPQIAPLPSSTLCPICKTTELI-SSPNQPNFNTCTQCHNKVCNQCGFNPNPHLTEVQEWLCLNCQMQRALGMDMTTAPRSKSQQQLHSPSLS---PSHSP--------------------------------------------------------------------------AKQPPPQP------------ 287mouse_Bassoon PTSPYSVPQIAPLPSSTLCPICKTSDLT-STPSQPNFNTCTQCHNKVCNQCGFNPNPHLTQVKEWLCLNCQMQRALGMDMTTAPRSKSQQQLHSPALS---PAHSP--------------------------------------------------------------------AKQPLGKPEQERSP------------ 264coelacanth_Bassoon PTSPYSVPQIAPLPSSTLCPICKTTDLN-SSPKQPNFNTCTQCRSVVCNQCGFNPNPHLTEVMEWLCLNCQMQRALGIDMTTATHSMSQQQIHSPSHS-APPQHKP--------------------------------------------------------------------------EPKSPAQP------------ 243frog_Bassoon PTSPYSVPQIAPLPSSTLCPICNTSDLT-SSPNQPNFNTCTQCHNEVCNQCGFNPNPHLTEVKEWLCLNCQMQRALGMDMTTATRSKSQQQLHSPILSPSQSPAKQTKPHASPQHTQPHTQPHTQPHTQPHTQPQTQQQ------------TQQQ--------------SQPQHQPLPKSQHQSQPQLLSQSHLQQQVHQ 270zebrafish_bassoon_a PTSPYSVPQIAPMPSSKLCPVCNTTELT-NFDGSPNFNKCTQCHTTVCNQCGFNPNPHLTGVKEWLCLNCQMQRALGMDMTT-PRSKSQQQIHSPSHQ-PKPDPIPKTQPSAQPTPQIQPQMQTQMKIQ----------------------------------------PQPHPQPQPHQQPQPHPQP------HPHPQP 308zebrafish_bassoon_b PTSPYSLPQIAPMPSSKLCPVCKTADLTGTTDGQPNCNKCTQCRSMVCNQCGFNPNPHLTEVQEWLCLNCQMQRALGMDMET-PRSKSQQQIHSPSQP-SKPSPQP--------------------------------------------------------------VTQPTAQPQPQPQPQPQPQP------------ 247stickleback_bassoon_a PTSPYSVPQIAPMPSSKLCPVCSSTQLS-NPPAPPNYNTCTQCKSTVCNQCGFNPNPHLTEVQEWLCLNCQMQRALGIDMTT-PRSKSQQQIHSPSHQ-AKPIVQPQQPTPQPTQPAAAAPSKPLAQSQPSQRQLQQQQHHPQQQQQQQQHHPQQHPQHQQHQSQPYSQSQPYSQSQPYSQPQQYPQS----QPQSYPGS 299stickleback_bassoon_b PSSPYSVPQIAPMPSSKLCPVCKTTDLMGTGDDKPTFNTCTQCCSMVCTQCGFNPNPHLTEVQEWLCLNCQMQRALGMDMTT-PRSKSQQQIHSPSHA-AKPELKPDASNLPETQTHSRPQTQPPAQDHLGQAHHPGQA-------------HPPGQAHPPGQAHPPGQAHPPGQAHPPGQAHPPGQA--HHPGQAHPPG 347fugu_bassoon_a PTSPYSVPQIAPMPSSKLCPVCTTTELS-NPPAVPNYNTCTQCKATVCNQCGFNPNPHLTEVQEWLCLNCQMQRALGIDMTT-PRSKSQQQIHSPSHQ-AKPIVQPQQPTPQPTQPTAVAQPKPPAHSQPTQQQHQQHP-------------------------QPYSQTQPFSQAQQYPQSQQYPSS----QSQPYAAS 335fugu_bassoon_b PTSPYSVPQIAPMPSSKLCPVCKTTDLMGVGD---NFNTCTQCRCKVCNQCGFNPNPHLTEVQEWLCLNCQMQRALGMDMTT-PRSKSQQQIHSPSQA-AKPELKS------------------------------------------------------------------DPSTQTAPQIQTPSQL------------ 243medaka_bassoon_b PTSPYSVPQIAPMPSSKLCPVCKNTDLMAAGDDKLSFNTCTQCHSMVCNQCGFNPNPHLTEVQEWLCLNCQMQRALGMDMDT-PRSKSQQQIHSPSHP-TNPDVKPESA------------------------------------------------------------AQPAPQTQ-----TPHPKP------------ 248medaka_bassoon_a PTSPYSVPQIAPMPSSKLCPVCTTTELS-NPPAVPNYNTCTQCKATVCNQCGFNPNPHLTEVQEWLCLNCQMQRALGIDMTT-PRSKSQQQIHSPSHQ-AKPIVQPQQPTSQPTQPVAAAQSKPMAQAQSVQQQQGQSQ--------------------------PYRQPQPHSQPQPYSQTQQYLQS----QNQSYPSS 315tilapia_bassoon_b PTSPYSVPQIAPMPSSKLCPVCKTTDLMGTGDDKPNINTCTQCRSMVCNQCGFNPNPHLTEVQEWLCLNCQMQRALGMDMTT-PRSKSQQQIHSPSHA-TNPDPKP------------------------------------------------------------------NASAQPAPQTHTPPQT------------ 256tilapia_bassoon_a PTSPYSVPQIAPMPSSKLCPVCTTTELS-NPPAVPNYNTCTQCKATVCNQCGFNPNPHLTEVQEWLCLNCQMQRALGIDMTT-PRSKSQQQIHSPSHQ-AKPIVQPQQPAPQPTQPTAAAQPKSLAQSQPTQRQQQQQQ----------------------------PQSQPYSQTQPYSQSQQYPQS----QQQSYASS 297cod_bassoon_a QNQPYSQAQSQPHSQAQSQPYSQAQSQP-HPQGQSQPHPQGQSQHHPQGQSQHHPQPY--------------SQSQGQTQ---PYSQSQSHFQGQSQPYSQAQSQPYTQGQNQPYTQGQNQPYTQGQNQPYTKGQTLQG------------QNQPYTQGH---------SQPYPQSQPHSQPFPQPQP----QPQTYPSS 266cod_bassoon_b PLLPYSVPQIAPMPSSKLCPVCNTADLMGSTDGQPNHNTCTQCRSMVCNQCGFNPNPHLT--QEWLCLNCQMQRALGMDMTT-PRSKSQQQIPSPSQP-AKPQLTSDAP--------------------------------------------------------------PQPAAQPAQAPTQPPTP---------TPA 185

: .:. : * * . :: *..:: . . lizard_Bassoon --------Q-----AAIGPEKQPGTMQPG--------------------------------------------LRPGAGAVQLETPKPP-------PASAQREPHSQVQ-------------PKPAAPQEVVRSSPQHQQAKPSSTDQRK-----TVGDSPAKTPAPPPGTGAPEQPQEGLTGKLFGFGASLLTQASTLM 405mouse_Bassoon --------------RGPGATQS--------------------------------------------------------GPRQAEAARAT----------SVPGP------------------TQATAPPEVGRVSPQPPLSTKPSTAEPRPPAGEAQGKSATTVP---SGLGAGEQTQEGLTGKLFGLGASLLTQASTLM 363coelacanth_Bassoon ---------------TPGPERKAGATQQS----------------------------------------------ATQGGHMPQAPLPTQQVKQEVTPRAHPNQ---PQ-------------PKPAAQQEAVRSSPQHHQAKPTAAEQKR-----SVGESPAKAP---SGPSSNTQEQEGLTGKLFGFGASLLNQASTLI 358frog_Bassoon VQPHQHPPQ-----QPPQPETHQQKPQYQ-------------------SQVQSKLHAQNQQAQKPQSQHQSPLHIQAGKETSHGSARIE-----SIHSSASPLPQQAKIQPTQQREQISQAYPKSSVPQEVLRSSPQHQ-----PAEQRK-----TAGSSPAKTP-AASSHGAQDQTQEGLTGKLFGFGASLLNQASTLI 430zebrafish_bassoon_a QPHPQPQPH-----AVSGIQRQTGPTGPSQTGFYSGPQTGLQTQKEPPQAGHLQSGYQSEPQRQ--------VLGPKSPERGPQIGRIP-------HPGAVPLP---GL-------------AKAQSQSDLGRGSPVRI---GSQPDRTR-----SAGSSPAHHA----PS--HDPPQDGL-TKLFGFGASLLNQASTLI 457zebrafish_bassoon_b --QPQPQPQ-----HPPEAKKQTVPQAVP-------------------------------------------------------QSKPP-------APGAVPLP---GM-------------AKAPSQPDLSRSGPGFL-PQIPRQDQTR-----SAGSSPSRQP---------PPPEQPF-GKLFGFGASLLNQASNLM 346stickleback_bassoon_a KSGPQAPPH-----TSPGLQRHSGPGGSQ------------------TQTGSLQQGMRSDPYSQRDTGAPGAVGVPTGGPNQPGGPRIP-------HPGAVPLP---GL-------------ARAPSQPDLGRGSPMHQ-SMARHHDQTR-----SAGSSPAHRP----QSQAPPPAQDGL-TKLFGFGASLLNQASTLI 442stickleback_bassoon_b QAHPPGQAHHPGQTHPPGQAHHPGQAHPPGQAHHPGQAHPPGQAHHPGQAHPPGQAHHPGQAHHPGQAHPPGQAHSSPGFVKQAGPAGPGQQQIKLPPGAVALP---GM-------------AKAPSQPDLSRNSPAHQ-PQPPRHDQTR-----SAGSSPSRQP---------PPPEQTF-GKLFGFGASLLNQASTLI 515fugu_bassoon_a HSGLQTQAH-----TSPGLQRHQGPGGPQ------------------AQTGNYQQGVRSDSYSQKAP-------GPVTGPNQPGGPRIP-------HPGAVPLP---GL-------------TKAPSQPDLGRGSPMHQ-SVARHHDQTR-----SAGSSPVHRP----PSQAPPPAQDGL-TKLFGFGASLLNQASTLI 471fugu_bassoon_b --HPHTQAQ-----PIPIPTKKPGPAGP--------------------------------------------------GQQQ---AKLP--------PGAVPLP---GM-------------AKAPSQPDLSRSSPAHQ-PPNPRQDQTR-----SAGSSPSRQP---------PPPEQTF-GKLFGFGASLLNQASTLI 343medaka_bassoon_b ---LSAQTH-----PGPAPTKQTGP-----------------------------------------------------GQQQ---VKLP--------PGAVPLP---GM-------------AKAPSQPDLSRNSPAHQ-PQHPRQDQTR-----SAGSSPSRQP---------PPPEQTF-GKLFGFGASLLNQASTLI 344medaka_bassoon_a QPRPQVQPH-----ESPGLQRHTGPAGHQ------------------AQIGQSQQGMRSDPYSQRGPGPSGPIGGSMGGPGQLGGPRIP-------HPGAVPLP---GL-------------TKAPSQPELGRGSPMHQ-SMARHHDQTR-----SAGSSPAHRP----QSQAPPPAQDGL-TKLFGFGASLLNQASTLI 458tilapia_bassoon_b --QPPLQAH-----PVPGPTKQTGPAGA--------------------------------------------------GQQQ---ARLP--------PGAVPLP---GM-------------AKAPSQPDLSRSSPAHQ-PQHPRQDQTR-----SAGSSPSRQP---------PPPEQTF-GKLFGFGASLLNQASTLI 356tilapia_bassoon_a QLGSQTQSY-----TSPGLQRHPGPGGSQ------------------SHTGPSQQGMRSDPYSQTGPGAPGTIRGPTGGFSQPGGPRIP-------HPGAVPLP---GL-------------TKAPSQPDLGRGSPMHQ-SMAR-HDQTR-----SAGSSPAHRP----QSQA-PPAQDGL-TKLFGFGASLLNQASTLI 438cod_bassoon_a QAGPKPQPQ-----ASPGLQRHQGPHGPQ------------------PQTGPSQQGMRSDPYSQKGAGVQGSAGGPTGGLNQPGGPRIP-------HPGAVPLV---GL-------------AKAPSQPDLGRGSPMHQ---------------------PG--------------------GQM---GGPIMGGPHT-- 374cod_bassoon_b QPPPSPQAP-----QAAGPNRQPGPAAP--------------------------------------------------GQQQHTRVKLP--------PGAVPLP---GM-------------AKAPSQPDLARQPPAHL-GHNPRQDQTR-----SAGSSPSRQP---------PPPEQTF-GKLFGFGASLLNQASTLI 290

* . . . lizard_Bassoon SVQPEATPPSQQSPGKV---------------------------------------------------------------PPRIVFSDGSKEAGTK-APGT-------AP-------------------------------------------GTQGKAG---------------------------------------- 451mouse_Bassoon SVQPEADTQGQPSPSKG---------------------------------------------------------------QPKIVFSDASKEAGPR-PPGS-------GP-------------------------------------------GPGPTPG---------------------------------------- 409coelacanth_Bassoon SVQPETTPPTQQSPSKV---------------------------------------------------------------ASKVVFSDESTKAGSK-TMGP-------LS-------------------------------------------GMQGKGG---------------------------------------- 404frog_Bassoon SVPAETAPQSQQSPGKV---------------------------------------------------------------PPKIVFSDASKDSGTK-PQGP----------------------------------------------------MSAVPSR---------------------------------------- 474zebrafish_bassoon_a SVDPL-TGSSQPSPARNQSAQ-----------------------------------------------------------GTKVIFSDANSGASAKTTAGPPGAFKTGPG-------------------------------------------GVTVPKGPG-------------------------------------- 516zebrafish_bassoon_b SVDPVQTAPPTQPPT-----------------------------------------------------------------SPKPPQKQPGP-------PGP----------------------------------------------------GPGPAVG---------------------------------------- 382stickleback_bassoon_a NVDPLPTASTQPSPARGNVVFSNAASDNRQQQQAAPGTKPFSMGGPHGPTQMGGPQASSRMGGPQAPSQMGGPHNASKMAGPHVQSQMGGPYPPGQ-MGGPHAPSQTGGPYPPGQMGGPHAPSQTGGPYPPSQTGGPYPPSQTGGPYPPGQMGGPHAPSQTGGPYPPSQTGGPYPPSQTGGPYPPSQTGGPYPPSQTGGP 641stickleback_bassoon_b S----ETTQPQQQP-------------------------------------------------------------------PKTAPKPVGP-------VGP-------GP-------------------------------------------GAGKASG---------------------------------------- 547fugu_bassoon_a NVDPLPTAATQPSPARGKVVFSNAAPDNKQQQQGTPFTKPFGMGGPHAPASMSGPHAQSHMGGPQSQSQMG---------GPHAQTQMGGPHAPSQ-MGGPHAPSQRGGPTQMSQMGGPQQPSQMGGPHVSSQMGSPYQQSQTGAPR----MGGPHAPTQ---------------------------------------- 617fugu_bassoon_b S----ETTQPQQQP-------------------------------------------------------------------PKPTPKPGGP-------SGP-------GS-------------------------------------------GSVRPPG---------------------------------------- 375medaka_bassoon_b S----ETTQPQQQP-------------------------------------------------------------------PKPAPKPGGP-------PGP-------VS-------------------------------------------GASKPSA---------------------------------------- 376medaka_bassoon_a NVDPLPTASPQPSPARGKVVFSNAASDNRQQQQGATSTKLFGMGGPSAP-------------------------------GPQSQGQMRDPYQPSM-I-GPKTSSLTGVPHDPSQAGGG--------------------------------MSGPHAPAQ---------------------------------------- 553tilapia_bassoon_b S----ETTQPQQQP-------------------------------------------------------------------PKPAPKPGGP-------PGP-------GP-------------------------------------------GAGKPSG---------------------------------------- 388tilapia_bassoon_a NVDPLPTASTQPSPARGKVVFSNATPDNRQQQQGTTSTKPFTMGGPHAPSQMG---------------------------GPNVQSQMGGPYASSQ-MGGPHAPSQMGGLHAPSQMGGP--------------------------------MGGPHMPTQ---------------------------------------- 538cod_bassoon_a -----PSQKLQQQPK-----------------------------------------------------------------QQQQQQQPLHPQQESH-QQLP-----------------------------------------------------AHLQKG---------------------------------------- 410cod_bassoon_b T----ETTQPPPPP-------------------------------------------------------------------PKTAPKPVGP-------QGP-------AP-------------------------------------------EAGKPPG---------------------------------------- 322

: ****:. :* .**. ** *. .*** *****:**: **.*****.***:* : * .*. . * lizard_Bassoon ----------------------------------------------------TIPAAKPV-------------------------KAEQ------VIKPKE------VPKERVLCPLCKVQLNVG-SGEPSNYNNCTSCKQRVCNMCGFNPTPHLVEKNEWLCLNCQTQRLLEG-SLGDPA-PMPLPTPKQQ-------- 551mouse_Bassoon --------------------------------------------------AKTEPGARMG------PGSGPGALAKTGGTASPKHGRAEHQAASKAAAKPK----TMPKERASACPLCQAELNMG-SRGPANYNTCTACKLQVCNLCGFNPTPHLVEKTEWLCLNCQTKRLLEG-SLGEPA-PLPLPTPQQP-------- 538coelacanth_Bassoon ----------------------------------------------------STPASKQG-------------------------KPDQ------VVQPKE------VERPKVFCPLCKTELNVG-TNQPPNYNFCTACKIQVCNKCGFNPAPHLVEKKEWLCLNCQTQRLLTG-SLGDAPHPVSPASPKHQ-------- 505frog_Bassoon --------------------------------------------------VESSPVHKQG-------------------------KTE-------PVKPKD--------VPKVMCPLCKTELNVG-FSEPANYNTCTSCKKQVCNMCGFNPTPHLQEKNEWLCLNCQTQRLLEG-NLGDTP-PSLPPTSKQQGSPRHLPA 581zebrafish_bassoon_a -----------------------------------APLQKQPPQQNQQQQQQSPMHHQKG----------PKPQQLAGQHQTALQQQSPLKQ---PLTQPV------VQKPEVTCPLCKTAFNVW-STEAPNYRSCTQCHTEVCNLCGFNPTPHLVEKKEWLCLNCQTQRLMSG-GLD--PVPVAPSSPSRQ-------- 650zebrafish_bassoon_b ----------------------------------------------------TKPTAQPG-------------------------------------PPRQ----DIPAKPKVCCPLCKAELNVGDPAKTPNYNLCTQCHTQVCNMCGFNPTPHLTEKKEWLCLTCQTQRAMSG-SLGDITPPVP------P-------- 474stickleback_bassoon_a QTQSQIGGPYPSGQMGGTRTLNEMGGSQFSGPHASSQKQQTTPHQQGMPQKQSPLHHQKG-------PQGQQYQQTPTHLQKALQKQE--PV-VAPVPAPE------PVKPKVNCPLCKTELNIG-NSDPPNYNTCTQCNGQVCNLCGFNPTPHLVEKKEWLCLNCQTQRLMSGGGLDDPPLPVPHPSPKHQ-------- 816stickleback_bassoon_b -----------------------------------------------------APPAQQG----------PRAHA----------QQQQ------QNAPMD------STKPKACCPLCKTDLNIGNTAEQPNYNTCTQCHHQVCNMCGFNPTPHLVEKKEWLCLNCQTQRALSG-SLGDAAAPVA------Q-------- 647fugu_bassoon_a ------------------------------------QQQTSMPHQQGMPQQQLPGHLQKG-------LQGQQNQKTPAHMQKAPQKQE--PA-VAPVPAPE------PAKPKVNCPLCKTELNIG-SSDPPNYNSCTQCKSQVCNLCGFNPTPHLVEKKEWLCLTCQTQRLMSGGGLDEPPLPVPHPSPKHQ-------- 756fugu_bassoon_b -----------------------------------------------------PQPAQHG----------PRAQA----------QQPQHP------TAPD------PSKAKVCCPLCKTHLNTGNKSDPPNYNNCTQCHQQVCNMCGFNPTPHLVEKNEWLCLNCQTQRALSG-NLGDMPAPIA------Q-------- 475medaka_bassoon_b -----------------------------------------------------SHPAQQG----------PHAST----------QQQQQQQ-QQQRAPSD------PSKPKTCCPLCKTSLNVGNTSEQPNYNTCTQCHSQVCNMCGFNPTPHLTEKKEWLCLNCQTQRALSG-SLGDIPAPMA------Q-------- 481medaka_bassoon_a -----------------------------------KQQQKTTPHLQETQQQQSPAHHQKGQQAKQPQSQGQQHQQTPSHEQKVPQKQE--PS-AAPAVIPE------PEKPKVNCPLCKTELNIG-SSEPPNYNSCTQCHSQVCNLCGFNPTPHLVEKKEWLCLNCQTQRLMSGGGLDDPPLPIPHPSPKHQ-------- 700tilapia_bassoon_b -----------------------------------------------------PQSAQQG----------PRPPS----------QQQQ------QHAPPD------SSKAKASCPLCKTNLNIGNTAEQPNYNTCTQCRSQVCNMCGFNPTPHIVEKKEWLCLNCQTQRALSG-SLGDIPAPVP------Q-------- 488tilapia_bassoon_a -----------------------------------KQQQQTMPQQQEM-QKQSPAHQQKGQQGQKTQPQGQQQQKTPTHQQKVQQKQE--PVAAAPAVAPE------PVKPKVNCPLCKTELNIG-SSNPPNYNTCTQCHSQVCNLCGFNPTPHLVEKKEWLCLNCQTQRLMSGEGLDEPPLPVPHPSPKHQ-------- 685cod_bassoon_a ------------------------------------------------SQQKLPAHQQKG-----------------------PQQQQQLPA-AVPAAPAE------PQRARVSCPLCKTELNIG-SADPPNYNSCTQCHSQVCNLCGFNPTPHLVEKKEWLCLNCQTQRLMSGGGLDEPPLPALHPSPKHQ-------- 523cod_bassoon_b -----------------------------------------------------PPHAQQG----------PHAPPQPQQQQQQQQQQQQQPQ-QQQRQPPQQPHQDPPSKPKACCPLCKTSLNVGNTAQPPNYNDCTQCHSQVCNKCGFNPTPHLVEKNEWLCLNCQTQRALSG-SLGDIPPPAP------Q-------- 443

. * : lizard_Bassoon --------------PTGSPRHQ--PGGT--GQQQRGP--LPHVTPQHP------PPSTLQDRLPSHLRTSEQS------------------------------KVPNTVPEKRPPAL------------------------------------------------------------------------------SEEKP 617mouse_Bassoon --------------PAGVPHRA--AGAA--PLKQKGPQGLGQPSGSLPAKASPQATKASPQATKASPQATKASPQTTK-------------------------ASPQAKPLRATEPS-------------------------------------------------------------------KTSSSAQEKKTVTSAK 628coelacanth_Bassoon --------------PMGSPKHQPVGFSQ--QQQQQQIRATQQQEMGFG--QQQKGVTQQQGRGLAQQQKEATQQQGISQ------------------------QQPSVGQSGQLPAA-------------------------------------------------------KHPGSSAQSKLMVQQTPTTASDTQKQQT 608frog_Bassoon LPTTGTPKHSQGAPTTGSPRHQTPSQTA--SPQHQKPGHMQTSSPQHQKQGPISTASPQHHIGPAGLHQKSVSQAITG-------------------------QQPGATQDKHIFQS-----------------------------------------------------------------DHLRSAEQGKIHTLNEDK 689zebrafish_bassoon_a --------------PLGSPRHQQISSKQQGTPQQKPPVSQQSAFSSDM-------------------------------------------------------KTSTALTTTTTPPV---------------------------------------------------------------------------------SI 700zebrafish_bassoon_b --------------SVGSPRLPGPANQQ---QQQRGSSQQASLRPTGP---QQQKPAGAQVSVPFSPVKQG--------------------------------APQSKGPSQQLPAT--------------------------------------------------------------------------------KTD 542stickleback_bassoon_a --------------PMGSPRQQAPASQP--SPLHK-PTNQQGPRPTQP--PAQKALAGTGGSGSFAPSAAKQPT-----------------------------DPKTATPATAPLST-------------------------------------------------------------------------------ADTQ 889stickleback_bassoon_b --------------PLGSPRPPATANQQ--PPQQKGPNQLLGPRPTGP--QQQQKPPGPQVSGPVSPVKHAGPAPHTKGPSQALPQTKGSAQPGSQNKGPNQQPSQTKGPSQPSAQS--KGPQSKGPNQSQGQSKGPVQSSNQMKGQGQAKGPTQTKGSAQPSAQTKGPSHPPGAKAP--PGKATPNHAKASPTPSKTTP 825fugu_bassoon_a --------------AMGSPRHQATTAQQ--SPLHK-PTSQQGQKPAQA--QVQKPPMGTDGSSPFAPTAAKQQA-----------------------------DTKTGTPATAPGPA-----------------------------------------------------------------------------PTTETQ 831fugu_bassoon_b --------------PIGSPQPSATSGQQ--PSQQRGSSQLTGPQPTGL--QQHKKATGPQVSGPVSPVKLSGPASQTKGPSQASPQAKGSAQP----KGATQPAPQSKGPTQPLAQS--KGPQIKGPNQLNSQSKGIVSSSNQMKGPGQAKGLTQTKGSAQPSAQAKGPSHPTGSKVSSATGKTTTNNSKAITTQSKTAP 651medaka_bassoon_b --------------PMGSPRASAPSNQQ--TAQQKSPSHLSGPRATVP--QQQQKPTGSQVSGPLSNGKEAEPASQTKRFSQTSPQTKCSTQPTSHVKGQTQPSAQSRGPAQAKGPN-----------MSHAQSKPPTQPANQTKGPGQVKGLTQTKGSAQPSAQTKGSSSSSGAKTSSSLGKASASNAKTTTTPSKTTP 652medaka_bassoon_a --------------QMGSPCHQTPTSQQ--SPLHK-PTTQQMAKPSQP-QTQKTTQSATSVSGPSITTTAKQPT-----------------------------ETKTTAPAPTAVPA-------------------------------------------------------------------------------TEAQ 774tilapia_bassoon_b --------------PMASPRSPVPANQQ--PPQQKGPNQLSGPRATGP--QQQQKPAGPQVSGPLSNVKQAGPTSETKG----SAQLKSSTQPPSQAKGPTQPTAQSKGPGQVKGPT-----------QSHAQSKVPAQPANQTKGPSQAKGLTQTKVSAQPSAQTKGP---SGAKTSSTPGKAAPSNAKASSTPSKTIP 652tilapia_bassoon_a --------------PTGSPRHQTPISQQ--SPLHK-PTTQQGSKPGQPQTQSQKPQAGSVVSSPFAPTAAKQPT-----------------------------ETKTTAPSTTPVTT-------------------------------------------------------------------------------TEIE 760cod_bassoon_a --------------SMGSPRHPSHVGQQ--SPLAKATTNLQGPKASQP--QQPKAHTGPGASGPFAPSAAKQQ------------------------------PATTATPSTTTTTT-----------------------------------------------------------------------TSAVPVLPSEGL 604cod_bassoon_b --------------PTGSPRTAVPATQQ--SPHQKTPNQLPGHRPTGP--QQQQKPPGAQVSGPISSVKKAGPTPQPSS------QTKGVAQPDLQPKSSTQSSPQSKVPSQTSSPSMVSSPQTRTSNQSSAQNKSSALSANQGRGPSQGKVPSQAK--AQISAQNKGP-HLSGAKPPATAGKAGPSSTKSGAAPAKAGP 616

. . : . . . : .:*. * *********:**.:.**.* : .: ::. * .*: : .:: *: : . *:*: ..::: . lizard_Bassoon LPKLPEEPPKASEGP--------LPKGKSMVTKPDTDFKDSTIISESQK-----SREQED-SSKPYPSDLSRSP-QSLSDTGYSSDGISSSQSEITGLV-QQEGEQLNETGIAEP-SPPSPSELTKLESSMRPLLESKGLTQEPTDRGKSYHELREEQKQRQRPRSLSITPE-AFDSDEELEDIMEEDEDSIEWENQREQ 799mouse_Bassoon AEPVPKPPPETTVPP-------GTPKAKSGVKRTDPATPVVKPVPEAPK-----GGEAEEPVPKPYSQDLSRSP-QSLSDTGYSSDGVSSSQSEITGVV-QQEVEQLDSAGVTGP-RPPSPSELHKVGSSLRPSLEAQAVAPSAEWSKPPRSSSSAVEDQKRRPHSLSITPE-AFDSDEELGDILEED-DSLAWGRQREQ 811coelacanth_Bassoon LLEKEKVLTPSQIGSSQKVIEHVPLKGTTSDAKTGVLVKESKQNVESQR-----VKHQEE-SSKPYPQELSRSP-QSMSDTGYSSDGISSSQSEITGLI-QHEEEKLSESGLKEA-SPPSPSELTKLESTVRPLLESKSISQDQSGRRRTFQDPREEQRQKQRPHSLSITPE-AFDSDEELEDILEEDEDSLEWENQRDQ 798frog_Bassoon KHPDETAQSKAFSDS-QTVSKQLVPKGKNLGNKKETGARNGKTPSELHK-----AKDQED-LSKPYSHDLSRSP-QSLSDTGYSSDGISSSQSEIAGLV-QQEEEKLSGTGISQR-SPPSPSELTKLESSVRPLLESNSINQGSN--GKMLHGSREDQKQKLRPRSLSITPE-AFDSDDELEDILEEDEDSLEWENQRDQ 876zebrafish_bassoon_a TAAFAGGEERKEYTP--KLDKELEDKENTLAQKRGTAESIQMISKDEKKELQSITKYYEETTKTNSNHDLSRSP-QSCSDTGYSSDGISSSQSEITGLI-QEEELKMNERGLTGHYTPPSPSELIKLESSMRPLLESKFSTDGDKKQDKKRGKQQHSDFRKHPSRSISISPE-DYDSDEDLEGIMEEEEDPTEE------ 889zebrafish_bassoon_b TKKQDQGNNKKQVMS-----KTEKDGDIKASSKKGT--------SDKKD-----EKKSSGIQKSKQDDDSNKSSTQSLSDTGYSSDGISSSQSEITGLI-QEDSMKLNERGVTDQRSPPSPSELTKLESSMRPLLESHTGTGQGQHRDGR------DNADQQRPRSLSITQDQEFESDEEAS-------DDLSC------ 704stickleback_bassoon_a KQPKAS-QEKPKTEP-----ENKIGKDTKASQRKGEQIT---PIKEIKK-----SRHYDD-TKNNSTHDLSRSP-QSLSDTGYSSDGISSSHSEITGLI-QEEEMKLSERGISGRGSPPSPSEITKLESSMRPLLEKSLSEEKVDRRGRKHRDRDLDDRGKQRPRSLSIPPD-SYDSDEELEDILEEEEDGGDW------ 1065stickleback_bassoon_b AQSKPTGNNQGRKQP--QEKAKVASKTVKEDVKASPKKILSETITSTKD-----MKIAEDTQKSRHHEDYNKSS-TSLSDTGYSSDGISSCHGEITGQI-REEGIQLSEKGAS------IPSEITKLESSMKPLLESKTSD------------------QKHRPHSLSVGQGRDYNPDDEAAR--DESEDELSC------ 984fugu_bassoon_a RPPKPT-EEKIRTEP-----ENQTAKESKPTPKKGEQII---PIKDLKK-----SRHYDD-TKNNSTYELSRSP-QSLSDTGYSSDGISSSHSEITGLI-QEEEMKLSERGVSGRGSPPSPSEITKLESSMRPLLEKSLSEEKPDRRGRRH--RDLDDRGKQRPRSLSIPPD-AYDSDEELDDILEEEESGGDW------ 1005fugu_bassoon_b VQSKPTGSTQARKQTQSQEKTEVLPKALKEVNKSSPKKTLSENMTSPKD-----VKTSEDTQKSRHHEDSNKSSTQSLSDTGYSSDGISSSHGEITGQI-IEEGIKLSERGAS------IPSEINILENSMKNLLESKTISD-----------------QKNRPLSLSVMLDREYSPEDDAAR--DESEDDLSC------ 814medaka_bassoon_b TQSKPTGISQARKQPQSQEKTKVAPKSLKEDVKVSTKKTLSENLTSPKE-----RTISEDAQKSRHHEDYNKSSTQSLSDTGYSSDGISSSQGEITGRI-QEEGIKLNERGSL------IPSEISKLETSITPLLQSKAAQD-----------------QKHRPHSLSVGQERDYNPEDDAAK--DESEDDLSC------ 815medaka_bassoon_a KQTKPT-QEKLKTET-----ENQTTKETKPSQKKGEQIT---PIKDIKK-----SRQYDD-TKNNSSHDLSRSP-QSLSDTGYSSDGISSSHSEITGLI-QEEEMKLSERGIIGRGSPPSPSEITKLESSMRPLLEKSFSEEKADRRGRKHRDRDLDDRGKQRPRSLSIPPD-AYDSDEELEGILEEEENVVDW------ 950tilapia_bassoon_b TQSKPTGNNQARKQPQSQEKTKVASKSLKEDVKASPKKALSETVTSPKD-----TKIAEDAQKSRHHEDSNKSSSQSLSDTGYSSDGISSSHGEITGQI-REEGIKLSERGAS------IPSEITKLESSMKPLLESKSASD-----------------QKHRPHSLSVGQD--ISPDDDAAR--DESEDDLSC------ 813tilapia_bassoon_a KQPKPS-EEKLKTEP-----ENQTSKETKPSQKKGEQIT---PIKEIKK-----SRHYDD-TKNNSTHDLSRSP-QSLSDTGYSSDGISSSHSEITGLI-QEEEMKLSERGISSRGSPPSPSEITKLESSMRPLLEKSFSEEKADRRGRKHRDRDLDDRGKQRPRSLSIPPD-AYDSDEELEDILEEEEDGGDW------ 936cod_bassoon_a KQPK-TGEEKAKTEP-----ESQLTKDPKMSQKKGEPIS---SMKEGKK-----SKQYDD-TKNNSTHDLSRSP-QSLSDTGYSSDGISSSHSEITGLIHEEEEMKLSERGISGRGSPPSPSEMTKLESSMRPLLEKSFSEEKSDRKGRRH--RDLDDRGKQRPRSLSITPE-AYDSDEELEDILEEEEDARDW------ 779cod_bassoon_b NPPKPTGNVQKKQGP---EKTKVTAKSQKDEVKPSSKKAVSVAISSTKD-----QRITETSQKSRHHEDSNKSSTQSLSDTGYSSDGISSSQGEIVGQI-WEEGIKLNEKGTS------RHPEINKLESSMRPLLEAKTAD------------------KKQRPHSLSVGHEPAYNSDEEAAR--EESEDELSC------ 775

. : . . . :: *: ****::****:*: : . . :*:* . ::: . . *********:**.*..: * ***************:**:*: ****.lizard_Bassoon RDSMESSDEFGSKLRHDYVEDSSEGGFSPLPTRQKVKQPQMTDEEFMRRQLLEMSAEEDN--LEEEEEEEYDREK-----YS---IIKNGHKP--DSEKISSKRRLPHSSGSIYEEERKELES---TESD----DVTASQGGLRRFKTIELNSTNNYGRDMEISQEADISLDREPELEMESLTGSPEDRSRGEYSSTLPA 980mouse_Bassoon QDTAESSDDFGSQLRHDYVEDSSEGGLSPLPPQPP-ARADMTDEEFMRRQILEMSAEEDN--LEEDDTAVSGRGLAK---H----SAQKASARPRPESSQEPKRRLPHNATTGYEELLSEAGPAEPTDSS----GALQ--GGLRRFKTIELNSTGSYGHELDLGQGPDPNLDREPELEMESLTGSPEDRSRGEHSSTLPA 995coelacanth_Bassoon RESMESSDDFGSKLRHDYVEDSSESGFSPLPTRPKNVDLELTDEEFMRRQLLEMSAEEDN--LEEEDMYSPPK-------HSK--KLHKHESE--KSKDGSSKRHLPHHASSMYEEETQGPEDSYKTAEE---EEMMSAKGGLRRFKTIELNNTNSYSREIELSSETDLSLDREPELEMESLTGSPEERSRGDYSSTLPA 982frog_Bassoon RDIVESSDDFGSKLRHDYVEDSSE-GFSPLPTRQQKG--EFSAEDFMRRQILEMSAEEDN--LEDEEDEYSHNK------YSSGKNGQKANSE--KVKGTSSKRRLYNSNNN-YDESRKETEE---EEEE----DMSSSQGGLRRFKTIDLNSTNSYNRDIDLEHEKDICLEREPELEMESLTGSPEERSRGEYSSTLPA 1055zebrafish_bassoon_a ------------ELRA-----ISALTENSGHKKRNVESEEMTDEEFMRRQIMEMSADEEN-EDEDEEEEMEEEDEDEDEGYGYK-KLKKTQKH--ISDSGKEKRRLTYHSSS-FEEESKTTSDIYKGSAE---EDLMASQGGLRRFKTIELNNTNSYSRDMELGTDNDLSLDREPELEMESLTGSPEERSRGDYSSTLPP 1064zebrafish_bassoon_b ------------KLRHDYVEDSSESGLSPLPPRQKKSQKELTDEEFMRRQILEMSADEED--TAD---------------TESHVKSKRSHKH--SGDLGKERRRLTQQSSS-FEEDSKSADNYKSAEAD----DMMASQGGLRRFKTIELNNTNSFNRDMELSTDHDL---REPELEMESLTGSPEERSRGEYSSTLPA 865stickleback_bassoon_a ------------EVKR---KEGKEEKKEKKKKEKETEPTEMTDEEFMRRQIMEMSADEDN---EEEEDEMEEEEDEEDEGYGYQ-KPKKNHHKHMISDSGKEKRRLQHHSSS-FEEEPKSSTDVYKGSLEEGEEDVMASQGGLRRFKTIELNNTNSYSRDMELGNENDLSLDREPELEMESLTGSPEERSRGDYSSTLPA 1245stickleback_bassoon_b ------------KLRHDYVEDSSESGLSPLPVRQKKSHKDLTDEEFMRRQILEMSADEEE--MEE---------------HGNQ-KSKRVHKT--GGDLSKERRRLAHQSNS-FEEDTKPTEA---NEEE----DVVMA-GGLRRFKTIELNNTNSYNRDMELSTEHDL---REPELEMESLTGSPEERSKGDYSSTLPA 1140fugu_bassoon_a ------------ESKR---KDVKEDKKDKKKKEKDLDPTEMTDEEFMRRQIMEMSADEEIEEEEEEEEEIEEDEYEEDEGYGYQ-KPKKSHHKHMISESVKEKRRLQHHSSS-FEEETKSSTDIYKGSVEEGEEDVMASQGGLRRFKTIELNNTNSYSRDMELSTENDLSLDREPELEMESLTGSPEERSRGDYSSTLPA 1188fugu_bassoon_b ------------KLRHDYVEDSSESGLSPLPIRQK-THKDLTDEEFMRRQIMEMSADEEE--VEE---------------HGNQ-KTKKVHKA--SGDL-KERRRLTQHSNS-FEDDTKAADSPYKANEE----DVVIA-GGLRRFKTIELNNTNSYNRDMELSTDHDL---REPELEMESLTGSPEERSKGEYSSTLPA 971medaka_bassoon_b ------------KLRHDYVEDSSESGLSPLPVRQKKSHKVLTDEEYMRRQIMEMSADEEE--SE----------------YGNQ-KAKKPHKT--SGDASKERRRLSHQSNS-FEDDTKGSEE---ASKD----DVVMA-GGLRRFKTIELNNTNSYNREIELSSEHDL---REPELEMESLTGSPEERSKGEYSSTLPA 970medaka_bassoon_a ------------EGKR---KEGKDEKKEKKKKEKEAEPTEMTDEEFMRRQIMEMSADEDI----EEEEEMEEEDDEEDEGYGYQ-KPKRSHHKHMISESGKEKRRLQHHSSS-FEEETKSSTDVYKGSLEEGEEDVMASQGGLRRFKTIELNNTNSYSRDMELNNENDLSLDREPELEMESLTGSPEERSRGDYSSTLPP 1129tilapia_bassoon_b ------------KLRHDYVEDSSESGLSPLPVRQKKSHKDLTDEEYMRRQIMEMSADEED--LED---------------YGNQ-KVKKSHKT--SGDLNKERRRLSHHSNS-FEDDAKVSEGAYKANEE---EDVVIA-GGLRRFKTIELNNTNSYNRDMELSTEHDL---REPELEMESLTGSPEERSKGEYSSTLPA 973tilapia_bassoon_a ------------DGKR---KEGKEEKKEKKKKEKEAEPTEMTDEEFMRRQIMEMSADEDN--EEEEEEEMEEDEDEEDEGYGYQ-KPKKSHHKHIISDSGKEKRRLQHHSSS-FEEETKSSTDVYKGSVEEGEEDLMASQGGLRRFKTIELNNTNSYSRDIELSNENDLSLDREPELEMESLTGSPEERSRGDYSSTLPP 1117cod_bassoon_a ------------EAKR---KEAKEEGKKEKKKEKDAEPPPMTDEEFMRRQIMEMSADEEN--EEEDDEEVAEAEDEEDEGYGYQ-KPKKAHLKHLPADTAKEKRRLQHHSSS-FEEDSKSSSDIYKGSAEEGEDDQMSSQGGLRRFKTIELNNTNSYSRDMDLANENDLSLDREPELEMESLTGSPEERSRGDSYSTLPP 960cod_bassoon_b ------------KLRHDYVEDSSESGLSPLPVRQKKTHKEMTDEDIMRRQIMEMSADEEE--VDE---------------YGNQ-KPKTAHKS--GGESGKERRRLTQQSNS-FEDDTKGADG---EDAE----GMLSSQGGLRRFKTIELNNTNSYP-------EQDL---REPELEMESLTGSPEERSRGEYSSTLPA 925

::.** ** ****:**:*:********* :***.************.***********:*:***********:**::***:****.***:***********************************************.***** * **:.* : ::**:*.*:***:* ***** *.. .: lizard_Bassoon TTPSYTSGTSPTSISSLEEDSDSSPSRRQRLEEVKQQRKARHRSHGPLLPTIEDSSEEEELREEEELLREQEKMREVEQQRIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSP--SIDSEAEGFDVIASKLYKSGSEYNLPTFMSLYSPTEKTASSAY-----PS--SKP 1171mouse_Bassoon STPSYTSGTSPTSLSSLEEDSDSSPSRRQRLEEAKQQRKARHRSHGPLLPTIEDSSEEEELREEEELLREQEKMREVEQQRIRSTARKTRRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSPSPSLDSEAETLDGGPTRLYKSGSEYNLPAFMSLYSPTETPSGSST-----TPSSGRP 1190coelacanth_Bassoon TTPSYTSGTSPTSISSMEEDSDSSPSRRQRLEEAKQQRKARHRSHGPLLPTIEDSSEEEELREEEELLREQEKMREVEQQRIRSTARKTRRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSP--SIDSEAEGFEIMSSKLYKSGSEYNLPTFMSLYSPTEKTLSNA------GYTSNKP 1174frog_Bassoon TTPSYTSGTSPTSMSSLEEDSDSSPSRRQRLEEVKQQRKARHRSHGPLLPTIEDSSEEEELREEEELLREQEKMREVEQQRIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSP--SIDSEAEGFELIGSKLYKSGSEYNLPTFMSLYSPTEKTSSTA-------S--NKP 1244zebrafish_bassoon_a TTSSYTSGISPTSISSMEDDSDSSPSRRQRLEEAKQQRKARHRSHGPLLPTIEDSSEEDELREEEELLREQEKMREVEQQRIRSTVRKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSP--SADSDAEGYEI-STKLYKSGSEYNLPTFMSLYSPTEKPETTSSTAAVTTSSSGKK 1261zebrafish_bassoon_b TTPSYTSGTSPTSVSSMEDDSDSSPSRRQRLEEAKQQRKARHRSHGPLLPTIEDSSEEDELREEEELLREQEKMRDLEQQRIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSP--SMDSEAEGFEM-GSKLYKSGSEYNLPTFMSLYSPTEKTSAVS------PSTADKS 1056stickleback_bassoon_a TSSSYGSGQSPTSISSMEEDSDSSPSRRQKLEEAKQQRKARHRSHGPLLPTIEDSSEEDELREEEELLREQEKMREVEQQRIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSASEYSPQ-SLDSEAEGYE---SKLYKSGSEYNLPTFMSLYSPIEKSSTTTTTTA--PSSASKS 1439stickleback_bassoon_b TTPSYTSGTSPTSVSSMEDDSDSSPSRRARLEEAKQQRKARHRSHGPLLPTIEDSSEEDELREEEELLREQEQMRDLEQQRIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSP--SMDSEAEGFEMIGGKLYKSGNEYNLPTFTSLYSPTEKTSLIS------PS--DKT 1330fugu_bassoon_a TSSSYGSGQSPTSISSMEEDSDSSPSRRQRLEEAKQQRKARHRSHGPLLPTIEDSSEEDELREEEELLREQEKMREVEQQRIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSASEYSPQ-SLDSEAEGYE---SKIYKSGSEYNLPTFMSLYSPVEKSSTTTTTTA--PSSTSKP 1382fugu_bassoon_b TTPSYTSGTSPTSVSSMEDDSDSSPSRRARLEEAKQQRKARHRSHGPLLPTIEDSSEEDELREEEELLREQEQMRDLEQQKIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSP--SMDSEVEGFDLIGGKLYKAGNEYNLPTFTSLYSPTEKTSVGS------TT--DKT 1161medaka_bassoon_b TTPSYTSGTSPTSVSSMEDDSDSSPSRRARLEEAKQQRKARHRSHGPLLPTIEDSSEEDELREEEELLREQEQMRDLEQQRIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSP--SMDSEAEGFEMIGGKLYKSGKEFNLPTFTSLYSPTEKTSAMS------PS--DKT 1160medaka_bassoon_a TSSSYGSGQSPTSISSMEEDSDSSPSRRQRLEEAKQQRKARHRSHGPLLPTIEDSSEEEEMREEEELLREQEKMREVEQQRIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSASEYSPQ-SLDSEAEGYE---SKLYKSGSEYNLPTFMSLYSPVEKSSTTTTTTA--PSSTSKA 1323tilapia_bassoon_b TTPSYTSGTSPTSVSSMEDDSDSSPSRRARLEEAKQQRKARHRSHGPLLPTIEDSSEEDELREEEELLREQEQMRDLEQQRIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSP--SMDSEAEGFEIIGGKLYKSGNEYNLPTFTSLYSPTEKTAVL-------PS--DKT 1162tilapia_bassoon_a TSSSYGSGQSPTSISSMEEDSDSSPSRRQRLEEAKQQRKARHRSHGPLLPTIEDSSEEDELREEEELLREQEKMREVEQQRIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSASEYSPQ-SLDSEAEGYE---SKLYKSGSEYNLPTFMSLYSPVEKTTATTTTA---PSSTSKP 1310cod_bassoon_a TSSSYGSGQSPTSISSMEEDSDSSPSRRQRLEEAKQQRKARHRSHGSLLPTIEDSSEEDELREEEELLREQEKMREVEQQRIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSASEYSPQ-SLDSEAEGYE---SKLYKSGSEYNLPTFMSLYSPVEKSSTTTTTVA--PSSTSKP 1154cod_bassoon_b TTPSYTSGTSPTSVSSMEDDSDSSPSRRAKLEEAKQQRKARHRSHGPLLPTIEDSSEEDELREEEELLREQEEMRDLEQQRIRSTARKTKRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSP--SVDSEAEGFEMIGGKLYKSGSEYNLPTFTSLYSPTEKSSGTS------PP--DKT 1115

******.**::*:**: :*.: .: *:**:****: :* :: : . : * . : lizard_Bassoon LKSAEEAYEEMMRKAEMFQKQQPSQQSISY-----SSTFQQVGY------RSSDSQNNFEYQYIDDYGYDNRGMDSSQSDYQNELSKSGAVYEEILQTSQNISRMHQSSSLDLTLEQEVKKKGREDLYQEKQFLNAESAY------ESGPLTPGTSPTQLSAPVSFS-ST--ESTTGRIIPDVRVTQHFAKESQEHSKLP 1351mouse_Bassoon LKSAEEAYEDMMRKAEMLQRQQGQVAGARGPHGGPSQP------------TGPRSQGSFEYQDTQDHDYGGRASQPVAESTPAGLG--AAVYEEILQTSQSIARMRQASSRDLGFTED--------KKKEKQFLNAESAYMDPMKQNGGPLTPGTSPTQLAAPVSFSTSTSSDSSGGRVIPDVRVTQHFAKEPQDPLKLH 1368coelacanth_Bassoon LKSAEEVYEEMMRKAEMLQKKQKQMQQPQQ--NIPSDAYLQDNY------RNTSNQNNFQYQYMDDYDNDAGSFRGS---YQNELSNSEQVYEEILQTSQSISRMHQSSSLDLSLKQEMKEPAADGAYQDKQLLDAESAYGELMKQNSALLTPGTSPTQLSAPVSFSSTD--VGPGGRSIPDVRVTQHFSKEGQEQPRAQ 1361frog_Bassoon LKSAEEVYEELMRKAEMFQKQQ-LQQLQQN--SMYGNNYQQVNY------IGTESQNNFEYQYIDEYSYDSGQIGSSQTEYHAGLSKPGTVYDEILQTSQNISRMHQSSSQDLSLQEDKKT----EQYPEKQFLNAENAYNDLIKQTSGSLTPGTSPTQLSAPVSFA-SS--DGHAGRVIPDVRVTQHFSKDGLAQTKLQ 1428zebrafish_bassoon_a LKSAEEVYEEMMRKAEMLQKQQKQQTQQQSSSSLILQQYSSATYQESDIRNRQDIEDEYDYNEQDDMHYENEET--------------VDIYEEIRQTSQNISKQL---DDQMEMDVS---------YNEKQLLDTGSAFAKLLEQSNALLTPGTSPTQLSAPVSFS--E--TGTGGR-IPDVRVTQHFSKDGPK-DRLR 1429zebrafish_bassoon_b LKSAEEVYEEMMRKAEMMQKQQQRAPGGPG--------------------------------------------------------------------QQNQERMLQTDKKQH------------------------------LDSGTTSLSPGTSPTQISAPVSFS--G--NDPSGR---GAQRMQSTQSHSQSQVRMN 1151stickleback_bassoon_a LKSAEEVYEEMMKKAEVLQKQQ-QQQQQQH---GRHGQYYEEDV------NGQGYEEEYEYD-QHQAGYDNEAAETE-----------KDIYEEIRQTSQNITKMQQATNEQVEIEIE-------SSYSDKQLLDTGSAFAKLLEQSNALLTPGTSPTQISAPVSFV------ETGGR-IPDVRVTQHFSAKDGHKDRIK 1603stickleback_bassoon_b LKSAEEVYEEMMKKAQLMQRQK--------------------------------------------------------------------------GTSQPDSKHH-------------------------------------LEAAGTVLTPGSSPTQVTAPMSFP--T--TGSDPR-IPGMHAAQHLSKETQN--RMM 1412fugu_bassoon_a LKSAEEVYEEMMRKAEMLQKQQ-KQQQQQQ--HGRQGQYFEEDI------NGQGY-DEYEYE-QDQNGYDNESMKTE-----------TDIYEEIRQTSQNITKMQLATDEQVEIDIE-------CSYPDKQLLDTGSAFAKLLEQSNALLTPGTSPTQISAPVSFA------ETGGR-IPDVRVTQHFSAKDGHKDRIK 1546fugu_bassoon_b LKSAEEVYEEMMKKAQLMQRRG-QTINQQQ------------------------------------------------------------------VASHTDNNHH-------------------------------------LE-AGTVLTPGSSPTQIAAPVSFS--T--TGSDQK-ISGIRAAQHLSKETQN--RMM 1249medaka_bassoon_b LKSAEEVYEEMMRKAQQMQRQG-QAVIQQK------------------------------------------------------------------GLSQSDSGHH-------------------------------------LE-SGTSLTPGSSPTQVTAPTTFP--T--SGNDTR-MTGIHAAQHLSKETQN--RMM 1248medaka_bassoon_a LKSAEEVYEEMMRKAEILQKQQ-KLQQQQQ--HGRHAPYYEEDI------NGQNYDDDYEYD-QDQTGYDDDSAEKE-----------TDIYEEIRQTSQNITKMQQATDEQVDIEIE-------SSYPEKQLLDTSSAFAKLLEQSNALLTPGTSPTQISAPVSFA------ETGGR-IPDVRVSQHFTSKDGHKDKIK 1488tilapia_bassoon_b LKSAEEVYEEMMKKAQLMQRQG-QAVNQQK------------------------------------------------------------------STSQSDDKHH-------------------------------------LE-AGTALTPGSSPTQVTAPMSFS--T--TGSDPR-IPGIHAAQHLSKETQN--RMM 1250tilapia_bassoon_a LKSAEEVYEEMMRKAEMLQKQQKQQQQQQQ--HGRHGQYYEEDI------NGQSYDDEYEYE-QDQTGYDNEAAETE-----------TDIYEEIRQTSQNITKMQQATDEQVEIEIE-------SSYPDKQLLDTGSAFAKLLEQSNALLTPGTSPTQISAPVSFA------ETAGR-IPDVRVTQHYSAKDGHKDRLK 1476cod_bassoon_a LKSAEEVYEEMMRKAELLQKQQ-KQQQQQH--HGRHGQYYEEEI------NGDEYEDEDEYS-QDRTGYDNEAAETE-----------QDIYEEIRQTSQNITKMQQASDAQVEIEIE-------SSYPEKQLLDTSSAFTKLLEQNNALLTPGTSPTQVSAPVSFA------ETAGR-IPDVRVTQHFSAKDVNKDRIK 1319cod_bassoon_b LKSAEEVYEEMMRKAQMMQNQG-QPVN---------------------------------------------------------------------STNQPVNKQH-------------------------------------LD-NSAELTPGSSPTQLNSPLSIS--S--TGNDPR-ITGIQGAQHLSKETQN--RMM 1200

. . : : . . *. ** .* .** . .: : *: lizard_Bassoon SSATAPSSVSKSVTTPYSYAKCSSTVTTVVSSPASAPRMYSTSSLSG-----------SDAPSVSTTSRSYSQTKMTANYGSQTENISRVKNMAEMSGSSAREKLQSMAESKTNVPMSTPKMYSFFKSSSPPLSPS-SPTQSPTHSSPRIGMPTSSGVEAKSTAEFSTQTHSTIIFYDVPTTSAAVSTSSPMVAQGTQT- 1538mouse_Bassoon SSPVSSTLTSKEVGMTFSQGPGSPATTASPTRGYMTPTSPAGSE---------------RSPSTSSTIHSYGQPPTTANYGSQTEELPHAPSGPPGSGRAPREKPLSGGDSEVGAPQ-PSRGYSYFTGSSPPLSPS-TPSESPTFSPGKLGP--------RATAEFSTQTPSLTLSSDIPRSPGP---PSPMVAQGTQT- 1539coelacanth_Bassoon GSALRTSSTSTVTTVPYMYSKGSNIVTAVSSTGQPSASISCTAVSYGRPCSIADSAISTEVASMTNRGVGYNQAKGTSTYYTQREGQLINRTHSEASGSPTKEIPQSSSESRCSTTL-SSKVFSFFKSSSPPLSPSNSPTQSPTHTSPRMGMSVSPVSGGKSTAEFSTQTYSTNITSVASNTTDVS-ITSPVTHQGVQT- 1558frog_Bassoon SSSIAPS--SKTISSNFQYGKGATTTTTSVVQTISSGAGITPLHS--------------GATPGPPTQRSYGQCKSASDF-KANEEQHRNRIAADLNAAS---KDNTQGDMRANVSL-TSKVISFFKSSSPPLSPS-SPTQSPTHSSPRISLVSGS----KATAEFSSQTQTTPASCDGSVPLSVV--SSPMIAQGTQT- 1599zebrafish_bassoon_a NQTGKNGITPTVAATTIAAYGVYARETVTVSQTTASQTISTTQPSLYGRQT----TTTTTVSNVSHKIAAITQAYNQRESAARKMAISRGVQMRDSSTSS---------DTRIESGPSSIRSYAYHERS-PPLSPTSSPTHSPTRSPSR-----------R-TAEFSTQTVSPSVLA-SSGNASH---TSPVMAQGTQT- 1598zebrafish_bassoon_b NQTSKGG-------------------------------------------------------HSGSPQSQIRQPAPTQPQGRPGVQGTAQGQVPDPGASSL-----------------TSKMFSYFKGSSPSTSPSTSPTHSPTRGPA---------------------------------PCTI---STVAPTQTSQS- 1242stickleback_bassoon_a TQAGKNGITPTVAATTIAAYGVYARDAVTISQTSSSPTMTTTQSGIYGRHTSSPTTSSTTVSSVCSKIAEITQAYSQREVITRRVGEARDVQVRDSSTSS---------TERVIEPR-SPKYTTYYRSTSPPLSPSPPPPQSPTRSPSR-----------RNTAEFSTQTFSSALRMSESAVSGP---TSPVTAQGTQT- 1778stickleback_bassoon_b TQSAKIE----------------------------------------------------GVVGVATTKAQVSQTATTRQAGSTVPGGNRGGSHRPTQASP---------DSAASSL--TSKVFSLFKGPSPPVSPNASPAQSPTHMSSV-----------RSTGGSGRQLPSL--------PGGP---TSATASHGAQA- 1526fugu_bassoon_a --AGKNGITPIVAATTIAAYGVYARDAVTISQTTSTHTITSSQSGLYGRQAGSPATSSATVSSVCSKIAEITQAYSQREVITKRVGDTKGSQVRDSST------------EQIIEPR-SPKYTTYYRSTSPPLSPS-PPPQSPTRSPSR-----------RGTVEFSTQTFSSALRM-ESAHSGP---SSPVMAQGTQT- 1714fugu_bassoon_b TQSAKIE----------------------------------------------------GVVGVATTKSQVSQAATTRQAGSTVPSNTRTDSRRPAQASS---------DSGASSL--TSKVFSLFKGPSPPVSPTISPVQSPTHMPSV-----------RPVGCTGRQLPPL--------PSGP---TSTSPSHGAQT- 1363medaka_bassoon_b TQSAKIE----------------------------------------------------GVVGVATTKAQMSQGAQTRQAGNTVTASSRASSQRPSQATQ---------DPGSASL--TSKVFSLFKGPSPPVSPTTSPTQSPMHTPSV-----------RPTSGSGRQLPSL--------PGAA---TTAPPSHGTQA- 1362medaka_bassoon_a GQSGKNGITPAVAATTIAAYGVYARDAVTLSQTASSHTITSTQSDVTRRQTGSGGTSSSTVSSVCSKIAEITQAYSQREVLTRRVGETRGVQVRESSTSS---------AERIIEPR-SPKYTTYYRSTSPPLSPSPPPPQSPTRVPSR-----------RATAEFSTQTLNSALRM-ESGTSGS---TSPVMAQGTQT- 1662tilapia_bassoon_b TQSAKIE----------------------------------------------------GVVGVATTKAA-SHTVTNRQTGGPVPAGTKVGSQRPTQAPS---------DPGTTSL--TSRVFSLFKGSSPPVSPTASPVQSPTHAPSV-----------RSTSSGGRQLPSL--------PGGP---TSAAASHGAQA- 1363tilapia_bassoon_a TQAGKNGITPTVAATTIAAYGVYARDAVTISQTGSSHTVTSTQSEIGRRHTGSTSASSATVSSVCSKIAEITQAYSQREVITRRVGETRGIQVRDSSTSS---------IERIIEPR-SPKYTTYYRSTSPPLSPSPPPPQSPTRSPSR-----------RATAEFSTQTFSSALRM-ESAGSGP---TSPVMAQGTQT- 1650cod_bassoon_a GQTGKNGMTPVVSATTIAAYGVFARDAVTISQTSTSHTVTTTSATHS-RHTGGPATSTTTVSSVCSKIAEITQAYSQREVITRRVGETRN--VRDGAASS---------TERIIEPR-SPKYTTYYRSTSPPLSPS-PPPQSPTRSPSR-----------RATAEFSTQTHTGGGL--QSAGSGP---SSPVLVQATQTS 1489cod_bassoon_b SQTAKIG-------------------------------------------------------GVVGKGQGQHSAQTARQAGSNSAIHSAGPQGQTQATSS---------ETGASL---SSKVFSYFKGPSPPVSPATSPSQSPTNSPSL-----------RSS---GRQLPSL--------PAGS---TAPAAPRGNQS- 1307

.. : * *::: . . .: .:: ** *** *** : :.:* *:*::******:* : * * : :: * :lizard_Bassoon PHRSSSPRLARQQSSQESPFMVITLASDVAIQTKPIGSGSSVSPASSPTQPSRQQTVHSYSQTV-INAAQLQQEQL-QSTYTKAQTVLERASAYTAAMQKVQS---PAAAADSFPGTYSWGTLPAENISLCRISSIPGTSRV-EPGPRPPGSNIVDLRTAIKSAPIIITDQGMDLTSLATESRKYCLTMDQSPGRQSTAI 1732mouse_Bassoon PHRPSTPRLVWQQSSQEAPIMVITLASDASSQTRMVHASASTSPLCSPTD--SQPTSHSYSQTT---PPSASQMPS-EPAGPPGFPRA------------------PSAGTDGPLALYGWGALPAENISLCRISSVPGTSRV-EPGPRPPGTAVVDLRTAVKPTPIILTDQGMDLTSLAVEARKYGLALDPVSGRQSTAV 1714coelacanth_Bassoon SHRSTSPRLTRQQSSQDSQFMVITLTSDASSPSRPLSVSSSISPLSSPTRLSRQQTIHGYTQTV-TSTSQPQQEQL-QFVYAKTTSQNERTSVCSSVAQMTFAKVHTDSTVVTTPSVYSRGALPMENISLCRISSVPGTSRV-EHSPRLPTGSAVDLRTAIKPAPIIVTEQGMDLTSVASESRKYS---DQLSNRHSTAV 1752frog_Bassoon PERTRAPRISRQQSSQEAPFMVITLASASSSQTKSKSVSSSTSPVTSPTRLNRQQTLHSYSQTSAASSQTPQDQS--QPSYSKVHTGIDRVPPAGMQMVTPKGQ--PSSVVDGSSSTYAWGSLPPENISLCRISSVPGTSRV-EPGPKHSG-SAVDLRTAIKPAPVIMTDQGMDLTSLATETRRYSLTMDQSPTRQSTTV 1793zebrafish_bassoon_a SHGVVTPGLFRQQSTQDTYYMIRTEEPEPTSPSKPVTVNTATSPLSSPTRFSRQSTFDAYSPPF-SPPDTP---PH-EQSPQHSLYRNYRAKKVN-----------VGTSMATTASMYSRGSMSMENISLCRISTVPGTSRI-EQGHRFQSASVVDLRTGIKPTPIIMTDQGMDLTSLATESRKYSGSLEGSPIRQSTTI 1781zebrafish_bassoon_b PHRSGAPRLARQQSSQDSPVIVITLGSGTSSQTKPATVNSSTSPLSSPTKDSQKTIYQTQHTTT-KSPTSPQKYPT-HQTSQHVLQRAQISERVS-----------LPASTAISAGAQSSGSLPAGNISLCRISTVPGTSRVVEQGTIAPGSNIVDLRAPMKPAPIIMTDQGMDLTSLASDARRYSLDSETAPSRH-TAV 1428stickleback_bassoon_a SHRSVSPRLHRQQSSQDVQY------SPPPGQARPMTVNTATSPLSSPTRFSRQSTFDNYSPCS-SPPDTP---PY-QHSPTRSFYRTQRVEKVN-----------VGTSMVTTAGTYTRGSMSMDNISLCRISTVPGTSRV-EQGRMIQGGSVVDLRTASKPAPTIMTDQGMDLTSLATESRKYHGGSDG--SRHSTIV 1953stickleback_bassoon_b PQRATSPRLARQQSSRDSPAMVITLASDTTSPAKPLTVNSSTSPLSSPTQVSCSSM-----GCG-------------------------------------------------------------------------------VKVQTHQISNVVDLRAPVRPAPIIMTDQGMDLTSLASDARRYSLGAEQPSGRH-TAV 1641fugu_bassoon_a SHHSISPRLHRQQSSQDAPY------SPPSSPARPMTVNTATSPLSSPTRFSRQSTFDTYSPCD-SPPDTP---PY-QQSPAHTFYRTQRVEKVN-----------VGTSMVTTATTYTRGSMSMDNISLCRISTVPGTSRV-EQGRMIQGGSVVDLRTISKPAPIIMTDQGMDLTSLATESRRYHGGSDG--SRHSTIV 1889fugu_bassoon_b PQRSISPRLVRQQSSQESPAMVVTLVSSTTSPSKPATVNSSTSPLSSPTQGTCRTLYSSQPPLT-GSPSSP--------------KMVQGVEKVN-----------VGI-STGNSIAHSRGSM--ENISVCKISNISGSSVMLGQGQIHSCTNVVDLRAPMRPAPIIMTDQGMDLTSLASDTRRYSLGGEQPHGRH-TAV 1533medaka_bassoon_b THRTMSSRLVRQPSSQDAPVMVITLGSETSSPAKPLTVNSSTSPLSSPTQAGGKTLYGSQPPVT-DSPSSPKMQHL-QQAQKNSSPKAQNMEKIT-----------VAVGAVTTSCG------SMEDTSPKKIIHFQGSSVVVGQGQTNPGTSVVDLRAPLRAAPIIMTDQGMDLTSLASDTRRYSLGTEQSAGKH-TAV 1542medaka_bassoon_a LPRSVSPRLYRQQSSQDASY------SPPTSPARPMTVNTATSPLSSPTRFSRQSTFESYSPCG-SPPDTP---PY-QQSPTRSFYRTQRVEKVN-----------VGTSMVTTASIYTRGSVSMDNISLCRISTVPGTSRV-EPGHLIQGGSVVDLRTATKPAPIIMTDQGMDLTSLATDCRKYHGGLES--HRHSTIV 1837tilapia_bassoon_b PQRAVSPRLIRQQSSQDSPVMVITLGSETTSPAKPLTVNSSTSPLSSPTQASCKTLYSSHPSST-SSPTSPQKHPS-QQSLKHSSQMAQNVEKVN-----------VGI---------SAGSV---EISLGKISNIQGTSKVIGQGETLPGTNVVDLRAPMRPAPIIMTDQGMDLTSLASDTRRYSLGAEQPAGRH-TAV 1537tilapia_bassoon_a SHRSVSPRLYRQQSSQDAPY------SPPSSPARPTTVNTATSPLSSPTRFSRQSTFDTYSPCG-SPPDTP---PY-HHSPTRSFYHTQRVEKVN-----------VGTSMVTTASIYTRGSVSMDNISLCRISTVPGTSRV-EQGHMIHGGSVVDLRTATKPAPIIMTDQGMDLTSLATESRKYHGGPEG--TRHSTIV 1825cod_bassoon_a SHRSVSPRLHRQQSSQDTRY------SPPSSPARPMTVNTATSPLSSPTRFSRQSTFEAYSPVA-SPPDTP---PFQQHSPTQSFYRTQRVEKVN-----------VGTSMVAPTNTYSRGSMSMDNISLCRISTVPGTSRV-EQGHMIHGGSVVDLRTITKSTPVIMTDNGMDLTSLATESRMYHGGPEG--GRHSTII 1665cod_bassoon_b PQKSSPPRLLRQQSSQETPVMVITLGSETTNAPKPITVNSSTSPLSSPTDVSGKPMYRSQPPST-TSPTSP------QQSPRHSSQN-------------------VNISTVNNASSYSRGSMSMENIS-----HNTGSSMVGGQGQVHPRGSVVDLSAPMRPAPIIMTDQGMDLTSLASDSRRYSIGAEQPSIRH-TAV 1475

*.: :**::* :.**:*:*:*.* : :: :: :* ** ::*:*:. .. :**: : : : . :.. . : ::* :.* . : . . * : lizard_Bassoon QPLIINLNAQEQPHAIISTATTVSITVASSMLVSQPKQPAVYGDPFQNRMDFGQGTGSPVCLTQIK------------QVEQ-AVQTGTFRGATSGPDMAASVGRPEAAGPQQ-------------AKFARYNLPNQMTS-LMKKDTLITQTTNAQSIISA--IPRQFPQDQS-------------AEVYR-GIPVEMKT 1889mouse_Bassoon QPLVINLNAQEQTHTFLATATTVSITMASSVLMAQQKQPVVYGDPFQSRLDFGQGSGSPVCLAQVK------------QVEQ-AVQTAPYRGGPRGRPRE--------------------------AKFARYNLPNQVTP-LARRDILITQMGTAQGVGLK-----PGPVPEP------------GAEPHR-ATPAELRS 1856coelacanth_Bassoon QPLIINLNAQEH---VVLSTTTVSITVAASMFVSHPKQPVVYGDPFQSRVDFGQGAGSAMCLTQTK----------LPEAAAQQTENMTNANTAGFMGKPDDLSGPQL------------------SKFARYNLPNQTSP-LVKKDMLMSQNNTPQSAVISAV---LRPLPQD--------QNVIPSDIYG-GVPLEMKI 1908frog_Bassoon QPMIVNLNAQEQPHAVIATATTVSITVASSMIVSQPKQPTIYGDPFQNRVDFGQGTGSAVCLTQVK------------QVDQ-ATATMCKPDDVVGIQKDI---------------------------FSRYNLANQVTP-IVKRD-GVSQPNNIQGVSNVSSVPRQYQQDHS----------TGPTEVYR-S-ATESKV 1939zebrafish_bassoon_a QPLIMNLNTQEQLHVVTSTATTVSVTVAASMFMSQPKHPVLYCDPLESRVDLGKDMGTVVCLAANK---PPPIDPSIPKIDA-KLEDLSIQQNQLLKQQQKLQQQQQLLEQQLQQHHQLQ--QQQAASFANLNISSQL-P-LFKKDMLVTQTSTATEVNV------ISPAMAS--------------DVYGVGGPLEFRA 1953zebrafish_bassoon_b QPLIMNLNSQAQPQVTTTTPTTVSVTVAASMFISQPKQPVVYGDPLQNRVDFGQGVGSAVCLTQNR---PLITDPSIPKIDA-CLENLGIQQQQLQKQQQKLQQQQELLEQQLQQHHQN-------ASFARYNLANQVQPILLKKDLVVSQNASGGTQTAVSSIAKISPLPPPPLSCSASTTPTLGHDIYG-GVALELKN 1616stickleback_bassoon_a QPLIMNLNTQE-----IITPTTVSVTVAASMFMTQPKQPTIYGDPHQYQMDLGQGIGSAVCLSQNRPPISPPTDPTIPKIDA-KLEDLSIQQRELQIQQEKLQKQQQLLEHQLQQHHQMQHHQQQASALARYSLTGQISP-LLKKDLLVSQTSTASAVVSA-----VSPVINP--------------DLYG-TGPVELKG 2126stickleback_bassoon_b QPLIMNLNAQEQPYISVSTPTTVSVTVAGSMFMSQPKHQVVYGDPLQHRVDLGQGVGSAVCLSQNK---PPITDPSIPKIDA-CLENLGIQQHQLQLQQQKLLQQQQLLEQQLQQHHQQ-------SSFARYNLANQVQPTLMKKDLVVSQTSSGQPAVTASAIPRVSPLVPPPV--SGAPASNTPLEVYS-GVALELKN 1827fugu_bassoon_a QPLIMNLNTQE-----TITPTTVSVTVAASMFMTQPKQPTVYGDPHQSRVDLGQGMGSVVCLSQNK-PISPPLDPSIPKIDA-KLQDLSVQQRELQKQQEKLQKQQELLEHQLQHHHQMQHQQQQASSLARYHLTGQISP-LLKKDMLVSQTSTASPVVSA-----VSPVINQ--------------DMYG-TGPIEVKG 2061fugu_bassoon_b QPLIINLNAQEQPCVSLSTPTTISITVAGSTFTSQSKQQIVYGDPLQNRVDLGQGVGSAVCLTQSK---PVVTDPSIPKIDA-CLENLGIQQQQLQLQQQKLLQQQQLLEQQLQQHQQ--------SSFARYNLANQVQPMLMKKDLVVNQTTNAQSVVTASTIPRVSPLASP-----LASGAAVSNEMYS-AVALELKN 1715medaka_bassoon_b QPLIMNLNAQEQPHVSVSTPTTVSVTVAGSMLMSQPKQPVVYGDPLQNRVDLGQGVGSAVCLTQSK---PAISDPSIPKIDA-CLENLGIQQQQLQLQQQKLLQQQQLLEQQLQQHQQQ-------SSFARYNLANQVQPLLMKKDIAVTQTSSAQPIVTASAILRVSPLAPPSI--PGGSTSHVPHEIFG-GVALELKN 1728medaka_bassoon_a QPLIMNLHTQE-----TTTPTTVSVTVAASMFMTQPKQPVVYGDPHQNRIDLGQGMGSAVCLSHNKSPISPPIDPAIPKIDA-KLEDLSIQQRELQKQQEKLQKQQEILEQQLQQHHQM---QQQVSTLNRYNLAGQISP-LLKKDLLVSQTSTASTVVSA-----VSPLINP--------------ELYG-TGPIELKG 2007tilapia_bassoon_b QPLIMNLNAQEQPHVSVSTPTTVSVTVAGSTFISQPKQPVVYGDPLQNRVDLGQGVGSAVCLTQSK---PPITDPSIPKIDA-CLENLGIQQQQLQLQQQKLLQQQQLLEQQLQQHQQQ-------SSFARYNLANQVQPLLIKKDLVVNQTSSAQPGVTASAIPRVSPLAPPSV--SGAPASNVPHELYG-GVALELKN 1723tilapia_bassoon_a QPLIMNLNTQE-----TTTPTTVSVTVAASMFMTQPKQPAVYGDPHQNRVDLGQGMGSAVCLSQNK-PVSPPTDPSIPKIDA-KLEDLSIQQRELQKQQEKLQKQQELLEQQLQQHHQMQQQQQQASVLARYNLTGPISP-LLKKDLLVSQTTTASAVVSA-----VSPIINP--------------ELYG-AGPVELKG 1997cod_bassoon_a QPLTMNLNTQE-----TTTPTTVSVTVAASMFMTQPQHPSVYGDPLQNRVDLGQGMSPAMCLSHNH-MVQPQIDPTIPKIDA-KLDDLSIQQQELQRQQEKLQKQQEILEQQLQHHHQMQHQQQHASAMARYNITGQISP-LLKKDMLVSQNGTAPTVVSA-----ISAPLNP--------------DLYG-NGPIELQG 1837cod_bassoon_b QSLVMNLNSQEQPHVAVSTPTTVSVTMAGSMFMTQPKQQVVYGDPLLNRVDLGQGVGSAMCLAQMK-----LTDPSIPKIDA-CLEDLGIQQHQLQLQQQKLLHQQQLLEQQLQQHQQQ-------SSFARYNLANQVQPMLLKKDIVVSQTSSAQPVVSVSAIPRVAPLPPQPA--TGAPASNQSHDMYT-GVALELKN 1659

.: ::*:: .:: :: : ::*: * : .: : .*** ** :**.. * .**.: * : : . lizard_Bassoon PAPPVAL----TSKKSQVMMVQMED---IAAGPITKLIKEEPP-----PNALDLT-G-MKTESQVACCDVVYKFPFAGSCTGA---FNPPSKVPEKNVGDPAHAGKPSG---P-YYG----KREQEVSEVYPYREQSAPGP-----NPYEEHRFYPHGLF--GRLYSSMSDTNL---SEMGLNYYSVKGEQPFHSLAGDS 2054mouse_Bassoon HAPP-------GTRKPHTVVVQMGE---GTAGTVTTLL-PEEP-----AGALDLT-G-MRPESQLACCDMVYKFPFGSSCTGT---FHPAPSAPDKSVTDTALPGQSSG---P-FYS----PRDPEPPEPLTFRTQGVVGP-----GPHEEQRPYPQGLP--GRLYSSMSDTNL---AEAGLNYHAQRLGQLFQGPGRDS 2017coelacanth_Bassoon HNPPLSH----SGVKAHAMMVQLDD---TAARTVTKLVKPEPP-----SNALDLT-G-IKPESHVACCDVVYKFPFGSSCTGT---FNPAPKMPEKSIVEPMQVGRPAA---PKYYG----SRTQELPDNYQYRE-PTGGS-----QVYHEQKPYPQGSF--GRLHSSMSDTNL---AEAGLNYYHTKPESQFHPHGGDS 2073frog_Bassoon PHASFNI----PGSQPHTMMVQLDD---MASGTVTNILREEQA-----SNALDLT-G-IKPESQVACCDMVYKFPFGSSCAGT----YNSSKIPETQTGEPSQAAR-SG---TQYYG----QREQDISDNYSYREAKPVLPAAPPHNLYEEQKFHPCGPP--GRLHSSMSETNL---SDIGL--YQP-----YHGTGGEA 2101zebrafish_bassoon_a KQAEAGVMNL-ANVKPQVMMVQIDG--AAQGATITQLVKPDEG-----QDSMNLT-GQIKSENQATCCDMVYNLPFGGSCVGG-------PFSQKPVTDEPSKQSSIAPTTLPQHYQ-----QFDLRQDGQTYQD-----------YTV---KSYTLPLP--GRLQPSMSETNL---SEAGLSSYSAKHDPHFHSSG-EL 2112zebrafish_bassoon_b KPAVINL----STVNPQVMMVQLEE-NSTQGATVTQLVKREEPPPP--VDVLDLT-GQIKPENQLACCDVVYKLPFAGSCSGP---------FSQRGKTTPSEPPAEPT---QVHGQ-----QSQPINDAYQTAT----IQ-----ATADESKSFTLPPA--GRLPPSMSDNNL---AGSSLQYYQR------TDPG-DL 1770stickleback_bassoon_a KPSPPGSIS--GSNLSHSMVVQMDG-GPEQARAMTQLLTVEVG-----QDAMDLTGGQIKPENQPACCDVVYRLPFGGSCVGG----------EKEGIRPPS-----AP---PLTYE------SDQERLPEGYHE-----------YPMDERKAYTLPFP--GRLQPSMSDTNL---AEAGVQSYHPKLEPHLQGPG-EL 2277stickleback_bassoon_b KPTVMNL----STGKPHVMMVQLDENKPSHGGTVTQLVKEEPPLP---PQVLDLT-GQIKPENQVACCDVVYKLPFAGSCSGSFNQKPPTISSDKTPTPESSQAAYPPH---PPHYNIIQQQQKQHQPQPTQ--------------RVTEEHKPYLPPVGSSGRIQPSMSDSNLPSVTDAG--HYQT------NIQG-GL 1993fugu_bassoon_a KPSSPDLMS--GGKSPHSMVVQIDG-GQLQTRTLTQLVKVEEG-----QDAMDLTEGQIKTDNQPVCCD-VYRLPFGGSCVGS---------AEKEGIRPPS-----AP---PLTYE------SDQERQPRSYHE-----------YPMDGHKAYTLPFP--GKLQPSMSDTNL---ADVGLQSYHS----HIQAPG-EL 2208fugu_bassoon_b KPTAVNL----STGKPHTMIVQLDETNVSQGSTVTQLVKPEETPS---PQVLDLT-GQIKPENQVACCDVVYKLPFAGSCS---------------------------------------------------------------------------------GRIQPSMSDTNLPSMTDTS--HHQN------NGQG-SF 1817medaka_bassoon_b KPTVMNL----STGKPHVMMVQLDESNVSPGGTVTQLVKQEEQPPPPTPQVLDLT-GPLKPESQVACCDVVYKLPFAGSCSGSFTQKPTPVSSEKNTPVNTSQAAKPTH----LFHDIINEKQQQQQAQPTKVLK--------------EEYKMYSLPS---ERIQPSMPDANLPSITDAV--NYQS------NAQG-GL 1893medaka_bassoon_a KPSPPGLMV--GGKTPHSMVVQMDG-GMELSRAVTQLVKVEQG-----QDAVDLTGGQITADNQPACCDVVYSLPFGGSCVGV---------PEKDGIKPPS-----AP---PIGYE------SEHERQPGRYHE-----------YPMDARKAYTLPYP--GKLQPSMSDTNL---ADAGLQSYYSKLEPHLQTSG-EP 2159tilapia_bassoon_b KPTVMNL----STGKPHVMMVHLDESNPSQSGTVTQLIKREEPPPP--PQVLDLT-GQIKPENQVACCDVVYKLPFGGSCSGSLTQKSPTVSSDKNTTTESSQAAHPPH---SPHYSISQQQQQQQQQHQPQVTQ-----------GMTDEHKAYQTPIVPSGRIQPSMSDTNLPSMTDAS--HYQS------NLQG-GM 1893tilapia_bassoon_a KPSPPGLMS--GGKSPHSMVVQMDG-GPDPARAVTQLVKVEEG-----QDAVDLTGGQIKPDNQPACCDVVYRLPFGGTCVGA---------PEKEGIRPPS-----AP---PLTYE------SDQERQPGRYHE-----------YPVDGRKAYTLPFP--GRLQPSMSDTNL---ADAGLQSYHSKLEPHLQASG-EL 2149cod_bassoon_a NPNTPGVMSLTGVGSPHSMVVQMDG-NIGPCGPVTQLVKVEEG-----QDAMDLAGGQIKSD-QPACCDVVYRLPFGGVCVGE---------AEKEGIRPPS-----AP---PINYE------SDPERQPGRYHE-----------YPMDGRKAYTLPYP--GRLQPSMSDTNL---SDPGLQAYHSKIDPQLQTTA-DL 1990cod_bassoon_b KPTVMNL----STGKPHVMMVQLDESNASQGGTISQLVKKEEPAPP--PQVLDLT-GQFKPESQAACCDVVYKMPFASSCTGSLTQRQTAATSDENSASDPSQVARPPP---APHYN-----TSQQQQQQAPAVQ-----------GLAEERKLYQPQPP--GGSQPSMSDNMLPSMTDVGPQLYQR------NHQG--- 1822

: :: . . ****::***: . *:**:.* .** ::: : . ::.**:*****************: :::.. :: . * : . : . . : . . : ::* * .: * **: ****:*lizard_Bassoon AVDLTTMKPSYSHSFTDGGYIGHGLQYGSFSDLRQPSDLLSHSYPMRRYSSASNIYSDYRFSPRGDMANFQEASLAHYSATTAREISRMCAALNSMDQYGGRHAN---SPDLLQYGHGGLGAGPGASGSMSTQQGV--MKANMMYNPNFPENRPGFGNL-----PQYNF--TRLGAVRQALPSTATVRAADGMIYSTINT 2242mouse_Bassoon AVDLSSLKHSYSLGFADGRYLGQGLQYGSFTDLRHPTDLLSHPLPLRRYSSVSNIYSDHRYGPRGDAVGFQEASLAQYSATTAREISRMCAALNSMDQYGGRHGSGSGGPDLVQYQP-QHGPG------LSAPQGLAPLRSGLLGNPTYPEGQPSPGNL-----AQYGPAASQATAVRQLLPSTATVRAADGMIYSTINT 2205coelacanth_Bassoon AVDLTTLKQSYSVSF-TEGYIGYGLQYGSFTDLRQPSEFISQSLPMRRYSSIPNIYSDYGYSSRGDLSAFQESSLAQYSATTAREISRMCAALNSMDQYGGRHTN---SPDLLQYGQDSISGGFTGNSGISTQQGLSSMKPNLMYNPNLSENCQTVTGQGYGTIGQYNVSGTRLGALRHMYPSVATVRAPDGMIYSTINT 2269frog_Bassoon AMDLSTMKNSFSMNFTDGSYLSQGMQYGSFTDLRQPTDLINSALPMRRYSSLSNIYSDYRYSSK-DLSNFQESNLAQYSATTAREISRMCAALNSMDQYGSRHPN---SADMLQYGASSMGSGPAAGNMLPNQQGMASVRQNMMYGQQLPEARQGFGNL-----TQYNISGLRAGTVRQMFPPAATVRAADGMIYSTINT 2292zebrafish_bassoon_a YTD--TMKDGY-----DGGYLGMGLQYGSFTDLRQ-GDIGD--LPIRRYSSLTNINSDYGYFSS-D---FAESNLAQYSATTAREISRMCAALNSIDRYSS-------NPDILQLGT-GRSSGP-PSR-VNLPQG---PRLGFKYSP---DRKPLSHSQ-----ALTDLINARQASLRAMYP--SAIRSADGMIYSTINT 2275zebrafish_bassoon_b AVDLSTMKQAY-----EAGYLGMGSQYGSYTDLRHQGSDSAPPLPLRRYGSMSNINAEYGYPPR-DLGGFQESNLAQYSATTAREISRMCAALNSMEQFGSRYTS---TPELLQYGA-GRG-GT-AGR-LNLQQGLASIRANLLYGP---DGRPTVHGQ-----TLTNLINARQASLRSLYP--PAGRTADGMIYSTINT 1947stickleback_bassoon_a AMDLTAMKQGY------GGFMGM--QYGSYTDLRHGGDITAQTLPIRRYNSLSNISSDYGYSAR-DIANFQEANLAQYSATTAREISRMCAALNSIDRYAS-------NPDLMQFGNLGRGTGPGASR-LAAGLG---MRQNHFFGL---DGKPISHSQ-----ALTNLINARQASLRAMYP--AAIRSADGMIYSTIQT 2447stickleback_bassoon_b AVDLSSMNQAY-----DGGYLGIGAQYGSYTDLRQQADITGPSLPLRRYGSMSNINSDYVYNSR-DQTGSQDSNLAQYSATTAREISRMCAALNSGDQFGSRYGN---IPELLPYGA-GRA-GS-LGR-LNLQQSLATIRANLLYGP---DGRPTANGQ-----TLTNLINARQASIRALYP--AAMRGGDGMIYSTINT 2170fugu_bassoon_a -VDPSAMKQGY------GGYIGL--QYGSYTDLRHGVDITAQTLPIRRYNSLSNINTDYGYTAR-EIASFQEANLAQYSATTAREISRMCAALNSMDRYGS-------NPDLLQFGSLGRGTGPVTSR-LAAGLG---IRQNFLLGL---DGKPISHSQ-----ALTNLINARQASLRAMYP--AAIRSSDGMIYSTIQT 2377fugu_bassoon_b AVDLSSMNQAH-----DGGYLGVGAQYGSYTDLRHQGDLAGPSLPLRRYGSMSNINSEYTYGSR-ELTGSQDSNLAQYSATTAREISRMCAALNSADQFGSRYGH---NIELGPYGT-GRG-GP-LSR-LNLQQSLASIRANLLYGT---DGRPTANGQ-----TLTNLINARQASIRALYP--AAMRGVDGMIYSTINT 1994medaka_bassoon_b AVNLSTMNQAY-----EYGALGMGAQYGSYTDLRHQGDLPAPSLPLRRYGSLSNINSDYAYTSR-DQTGLPDSNLAQYSATTAREISRMCAALNTVDHFGSRYGN---SPELASYGA-GRG-GP-LAR-LNLHQSLASIKANLLYGP---DGRPTANGQ-----SLTNLINARQASLRAMYP--TAVRGSDGIIYSTINT 2070medaka_bassoon_a AMDLTAMKQSY------GGYVGM--QYGSYTDLRHGGDIATQTLPIRRFNSLSNISSDYGYSAR-DIANFQEANLAQYSATTAREISRMCAALNSMDRYGS-------NPDLVQFGSLGRGTGPGASR-LAAGLG---MRQNLLFGL---DGKPISHGQ-----ALANLINARQASLRAMYP--AAIRSSDGMIYSTIQT 2329tilapia_bassoon_b AVDLSSINQVY-----EGGYLGMGAQYGSYTDLRHQGDLGGPSLPLRRYGSMSNISSDYAYSSR-DLTVSQDSNLAQYSATTAREISRMCAALNTVDQYGNRYGT---NAELP-YGA-GRG-GP-LAR-LNLQQSLASIRANLLYGP---DGRPTANGQ-----TLTNLINARQASIRALYP--AALRGGDGMIYSTINT 2069tilapia_bassoon_a AMDLTAMKQAY------GGYVGM--QYGSYTDLRHGGDIAAQTLPIRRYNSLSNISSDYGYSAR-DIANFQEANLAQYSATTAREISRMCAALNSMDRYGS-------NPDLMQFGSLSRGTGPVASR-LAAGLG---IRQNLLFGL---DGKPISHSQ-----ALTNLINARQASLRAMYP--AAIRSSDGMIYSTIQT 2319cod_bassoon_a AMDENVMKQAY-----EGGFMGM--QYGSYTDLRHGGDITAQALPIRRFNSLSNISTDYGYSAR-DIANFQEANLAQYSATTAREISRMCAALNTIDRYGS-------NPDLMQYGSLGRGSGPGTAR-LAASLG---IRPNLLFGP---DGKPMSHNQ-----ALTNLINARQASLRALYP--TAIRSADGMIYSTINT 2161cod_bassoon_b AVDLSTVNQSY------DGYQGMGAQYGSYTDLRQ-ADVGGSPLPLRRYGSMSNINSDYGYGP-----GVTDANLAQYSATTAREISRMCAALNSMDQYGSRFA----NPELMQYG--GRG-GP-VGR-LNLSQSLASIRANLLYGP---DGRPTAHGQ-----SLTNLINARQASIRALYP--AAMRGTDGMTYSTINT 1991

***:*:******:.: .*: * . * ** . :*.:**:.* *:* : .* . * : .:***: lizard_Bassoon PIASTLPITTQPASVLRPMLR-GLYRPYGPG-ITAVPLASLTRVPLVAPRMPLATQGLYRYPAPSRLPAAPTSS--------------------------VMETPMYLGKPGSTTTASTAASSVSAVPVSKPSAPTAVG-------LPRQEQTGTGIREETPVATSGAQSTISQPQQQQQPQQQQQPPPQIPI------- 2400mouse_Bassoon PIAATLPITTQPASVLRPMVRGGMYRPYVSGGVTAVPLTSLTRVPMIAPRVPLGPAGLYRYPAP-RFPIASSVP--------------------------PAEGPVYLGKPAAAKASGAGGPPRPELPAG----------------VAREEPFSTTAPAVIK-----EAPVAPAPGPAPAPPPGQKP------------- 2344coelacanth_Bassoon PIASTLPITTQPASVLRPMLR-SVYRPYGPGSITAVPLANLTRVPLVTPRMSLT-QSLYRYPPPNRLPTGPATS--------------------------VTETPMYLGKPTNTPSATVPANNVNT--VLPNTTPQTLNQ-TTANIQRLEQSNGNTHEAVTTTVPVAQRAPLESSQPQPPQQQQQPPLQQQPPLQQQPPL 2438frog_Bassoon PIASTLPITTQPAPVLRPMIR-GLYRPYGPGNITAVPLASLTRVPMVTPRMPLAPQGVYRYPGVSRFPAPSTAS--------------------------SMDTPVYLGKPAEAVTTSSAPIISSSAPVLPVSTAVSSVL-ASS--TVRTEHTTAVTRDDITPSVVQSNAFKDTSQPQQQKPAPEVPLSTSA-------- 2454zebrafish_bassoon_a PIASTVPITTQPSSVLRPLLR-GGYRPYPTPNMTPVPLASLTRLP-VAPR---TGQAPFQCPTPNPLPVTTSTKVPESPAITT-----------------VQDAPLYLGKSPVSITAAGTFAQPIQPSSVPISTPMNLAM-SQP--EVQAQPINLSQSHLQA----NTQPTLQHQLPQSNSSAQSHP------------- 2433zebrafish_bassoon_b PIASTLPITTQPAPVLNPMLR-GAYGPYTSGGVTAVPLASLTRLPQVTPRMPLNAQAPYRYPPPNQFSATAPETLSGSVPPSST----------------SQEVPVYLGKPTAPGSMSTNASQKSSQPVS----------------NMGMQSIQPAHTSMVSVQSVVNAPITHMPVDSTTMQLQSQPQTQAQSLVKGQEQ 2114stickleback_bassoon_a PIASTLPITTQPASVLRPLLR-GVYRPYPSANMTPVPLTSLSRLP-MSPRVPLTVQAPLRYPAPGLLQISSTTATSSGAATNVGPAGAPMSVSITTTSSTVQDEPVYLGKSVSSL------VEVQGTIAIPM--------------EQQQQPINLSQAHLNSQQQQQQQVASAQQSHQPPQAAHAYLSSTPSHTHSYTQP 2625stickleback_bassoon_b PIASTLPITTQPSSVLHPVPR-GIYRPYPTG-VTAVPLASLTRLPQVTPRMPLSTQGPYSYPTPNQYPTSATPSGSVPEASSS-----------------HQETPVYLGRPITTVSAQTVATIPPTQHAAQLGAQNVLATGMQNPANQQTDHTQLSSQSVSPFSQAQGQPPTVKP-LQVQMQPQQLPAQTEALSLTQTQP 2350fugu_bassoon_a PIASTLPITTQPASLLRPLLR-GIYRPYPSANMTPVPLASLSRLP-VNPRMPMAGQAPVRYPAPGLLQTTSVTATTGVAATTSAPIGAPVLT---TSSSTVQDQPVYLGKGV----ASAEVTSVPGTVVIPT--------------EPQQQPLNLSQAHLNS------QQLTTTQQSQQPPPAHSHPHSTLSHSHGYTQP 2548fugu_bassoon_b PIASTLPITTQPSSVHRPIPR-GIYRPYPTG-VTAVPLATLTRLPQMTTRMALSTQGPYSYPPANQLPTSDTSSGVIPDATTL-----------------HQETAVYLGKPSTTATVQAAASILPSQHVAATSLQTT---------IGQQMDHTLNTQSVTTFSQAHGHPPC-GQIVQAQMQPQHIPAQTEPLSLTQTQP 2165medaka_bassoon_b PIASTLPITTQPAPVLHPMPR-GIYRPYPTA-VTAVPLASLTRLPQMTPRMPLAAQGPYPYPTPNQYSSAAPPSGGVTAESSS-----------------HQETPVYLGKPLTTIATIQPIQSVVHLEAHDVPATSVQ--------STSDQQLDRTQFTSQSNTFKAQGQLSTAQSLQAQMPSQQLPTQSEPLSLTQTQP 2243medaka_bassoon_a PIASTLPITTQPASVLRPLLR-GVYRPYASPNLTPVPLSSL-RLP-MSPRIPLTGPAPVRYPAPGLLQTTSTTATTTVAAAASAPIGPLAFV---TT---IQDEPVYLGKGAKVTSSSAETPLIQGAVVIPS--------------EPQQQPINLSQAHLNTQQQQQEKPGTMTQQPQAPATSQTYPSSSPALTHGYPQP 2506tilapia_bassoon_b PIASTLPITTQPASVLRPMPR-GIYRPYPTG-VTAVPLASLTRLPQLTPRMPLSTQGAYSYPPPNQYQTSATPSGSMPVVSSS-----------------HQETPMYLGKPLTTVAPQTVTTIAPSQTAVQSVVATGMYT-TA---DHQIDHAKLSSQSITALSQAQSQPPTVQPVQ-----AQQLPAQTEPLSLTQTQT 2241tilapia_bassoon_a PIASTLPITTQPASVLRPLIR-GVYRPYLSANMTPVPLSSLSRLP-MSPRMPLSGQAAVRYPAPGLLQTTSATDISAAATTTSATAGALVGVSM-TTSSVVQDEPVYLGKGTTVSSSSAEVTSVQGTVVIPA--------------DQQQQPINLSQAHLSS--QQQKQVTTVQQPQQPAPAAHAYPLSGPAQTHGYTQP 2500cod_bassoon_a PIASTLPITTQPSSVLRPMLR-GAYRPYPPANMTPIPLASLSRLP-MNPRAPLSGQAPFRYPVPNGFQGIPATSAIVSTSSASATTTA------------VQDGPVYLGKGANIAPSSGAVSSTPGIVSMPTT-------------EPQQQPINLSQAHLNSQQQQQQQQQMAGSHQQQQFAHQTQVYNQTS-------- 2326cod_bassoon_b PIASTLPITTQPSSVLRPMPR-GAYRPYPTG-VTPVPLASLTRLPQVTPRMPLSTQGTYCYPTSNQYPTSIPGPGLPTSNTTS-----------------DQETPVYLGKPPTIGSTLPPTQTVAHLGAKHLSAPGIQTN-MIAF-EQINQQVQIPIQNVTS--LSQAQLPNEQNQMQEQLQSLQMPPQTQALSLTQTQS 2168

:: :***::*::***:****.:**:*::*****:* **: **::*::::::*::***.**.: :.: . . . :.:: ..:. .*:: : : *: : lizard_Bassoon --QKQQEAPQASA-----------ASKGA----------MEKEEKEKEEERLRKQQEHILQMERERVELEKLRQMRLHEELERERVELQRHREKEQLLMHREIQELQSIKHQVLQQQQEERQVQFVLQREQLAQQRLQLEQIQQLQQQLQQQLEEQKRQKGPFPPPVCDPAGRMPPQVTSEMPVEMQ------------- 2564mouse_Bassoon --AGEAVAGSGSGV----------LSRPA-----------SEKEEASQEDRQRKQQEQLLQLERERVELEKLRQLRLQEELERERVELQRHREEEQLLVQRELQELQTIKQHVLQQQQEERQAQFALQREQLAQQRLQLEQIQQLQQQLQLQLEEQKQRQKAPFPATCEAPSRGPPPAA--------------------- 2500coelacanth_Bassoon QQQRQQELQQSSAT----------TKSIA-----------EKEEKEKEEERLRKQQEQILQFERERVELEKLRQLRLQEELERERIELQRHREKEQMLVQREIQELQTIKQHVLQQQQEERQTQLALQREQLAQQRMQLEQIQQLQHQLQQQLEEQKRQKTT-LPPASDPTGRIPSQAIPEITVEMQ------------- 2603frog_Bassoon ------------------------QKPGA-------------EKEEKEEERQRKQQEQMLQIERERVELETLRQMRLHEELERERIELQRHREKEQMLVQRELQELQSIKHQVLQQQQEERHVQFALQREQLAQQRMQLEQIQQLQQQLQQQLEEQKRQKN--VPPACEQTGRLPPQTVSEIAIEMQ------------- 2602zebrafish_bassoon_a -------PVQGHPIATQGGRTTL-TAVRN-----------IASQENETEEKVHQQQEQLLQIERERVELEKLRQLRLQEELERERAELQRHREKEQILVQREIQELQSIKQQVLQQQQAERESQLILQREQLAQQKQQLDQIQSLQHQLQQQLEEQKRQKTVSGSTQ--------------------------------- 2581zebrafish_bassoon_b TQGQPQTQPQPLTL--PQKQTQAITRVFPDASASTTITTSADTEKEKEEERLRQQQEQLLQLERERVELEKLRQLRLQEELERDRVELQRHREKEHLLMQREIQELQTIKQQVLHQQQAERETQLVMQREQLAQQRMQLDQIHSLQQQLQQQLEEQKRQKTAAIEAAAVAAAAEAAAASAAAAAAATTAQGAMQGVMVGG 2312stickleback_bassoon_a P-VGPSVPTSGLPI--SGGLPPF-PPPGA-----------VHKEGETEAERLHRQQEQLLQMERERVELEKLRQLRLQEELERERIELKLHRDKEQMIVQRELHELQNIKDQVLQQQQLEREKQLVLQREQLAQQKSQLDQIQSLQHQLQQQLVEQKRQKTAAAQN---------------------------------- 2776stickleback_bassoon_b Q-IQPISQPQPLAL--PQGQPQG-TVHGAALPPSSAISPPVDAMKSKDDERLSQQQEHVLNLERERVELEKLRQLRLHEELERDRVELQRHREKEQLIVQREIQELQTIKQQSYTNSRQSGRHSSLCKENNWPSRECSLNN-SVFAAPAPAAVGGAKKTKDSCSGSSSY------------------------------- 2514fugu_bassoon_a P-AGHSASASGEGL--PGGLPPF-PPPGV-----------IHKEGETEAERLHRQQEQLLQLERERVELEKIRQLRLHEELERERIELKLHREKEQILVHRELQELQNIKEQVLQQQQHEREKQLILQREQLAQQKSQLDQIQSLQNQLQVQLEEQKRHKTVGQP----------------------------------- 2698fugu_bassoon_b QLTQDQTQVTTHSI----------ATSGS------KLSDSTDVPKSKDDERLSQQQEHMLQLERERVELEKLRQLRLQEELERDRMELQRHREKEQLLVQREIQELQTIKQQVLQQQQAERETQLIMQREQLAQQRMQLEQIQSLQQQLQQQLEEQKRQKTAAVEAVAAAAAAEAAAASAAASAAATSAQAPIQGVVVGG 2349medaka_bassoon_b R---PTSQPQSLAV--SQGESQA-TPHKDAQVTGPKLSPPVEVQKGKEEERLSQQQEHVLQLERERVELEKVRQLRLHEELERDRMELQRHREKEQLLVQREIQELQTIKQQVLQQQQAERETQLIMQREQLAQQRLQLEQIQSLQHQLQQQLEEQKRQKTAAVEAAAAAAAEAAASAAAAAATSAQ---GAIQGVVVGG 2434medaka_bassoon_a P-VGPSVPTAGLPS--AGGLPPF-PPPGA-----------IHKEGETEAERLHRQQEQLLQMERERVELEKLRQLRLQEELERERIELKLHREKEQMLVQRELQELQTIKEQVLQHQQHEREKQLVLQREQLAQQKAQLDQIQSLQQQLQLQLEEQKRQKSAQN------------------------------------ 2655tilapia_bassoon_b Q-AQPVSQPQQLVL--PQSQSQA-PPHGSAQAPSSKLSPPTDAQKGKEDERLSQQQEHMLQLERERVELEKLRQLRLQEELERDRMELQRHREKEQLLVQREIQELQTIKQQVLQQQQAERETQLIMQREQLAQQRMQLEQIQSLQQQLQQQLEEQKRQKTAAVEAAAAAAVAEAAAASAAAAAAATSAQGAMQGVVVGG 2437tilapia_bassoon_a P-AVLSAPTAGLPI--PGGLPPF-PPPGA-----------IHKEGETEAERLHRQQEQLLQMERERVELEKLRQLRLQEELERERLELKLHREKEQMLVQRELQELQNIKEQVLQQQQQEREKQLVLQREQLAQQKSQLDQIQSLQQQLQQQLQEQKRQKTAAVAQI--------------------------------- 2652cod_bassoon_a --AGPTGLSGGMPL--QGGLPPF-PPPGA-----------IHKEGETEEDRLHRQQEQLLQMERERVELEKLRQLRIQEELERERIELKHHREKEQMLVHKEIQELQTIKEQVLQQQQQERENQLVLQREQLAQQKSQLDQIQSLQQQLQQQLQEQKRQKTANAQG---------------------------------- 2476cod_bassoon_b P-SHIVTHPQPQALVQPQAQILVTAPVTASEATCSILSPPMDARKDKEEERLSQQQEHMLQLEREHVELEKVRQLRLHEELERDRMELQRHREKEQMLVQREIQELQTIKHQVLQQQQAERETQLIMQREQLAQQRMQLDQIQTLQQQLQQQLEDQKRQKTAAVEAAAASAAAEAAAGAAAAAAAATSAQGAMQGVVVGG 2367

.. .* *. . :. :. ::*: ** ** : : :: .*..****:*: * : :* * : * .*.**** *:**: : * ****** *:*: * : lizard_Bassoon -------RTLAQNGQYWPP--INQ-AFIATA--APDGQA---QPRYTGLQRPLTNSASEMSLQTEEQWETNR-GIKKRNSMPRL-RDAYDKEPGHEPYI-VKKITDSSVQTDEEDGEERLYASRRRRTR-RSTDCSVQT-DEEDNGEWEQ-PVRRRRSRFSRHSDSGA----------------------ESKQD----- 2716mouse_Bassoon -------TELAQNGQYWPP--LTHAAFIAVA--GTEGPG---QPREPVLHRGLPSSASDMSLQTEEQWEAGRSGIKKRHSMPRL-RDACEPESGPDPST-VRRIADSSVQTDDEEGEGRYLVTRRRRTR-RSADCSVQT-DDEDNADWEQ-PVRRRRSRLSRHSDSGS----------------------DSKHDATASS 2659coelacanth_Bassoon -------RTIAQNGQYWQP--ITQ-PFIEGIPVATAGHEVPMHPRYAGPQRPLPNSNSEMSLRNEEHWEV-R-GMKKRNSMPRL-RDAYEADDSRDQYF-VKKITDSCVQTDDEDGEGRYYISRRRRSR-RSVDCSVQT-DDEDNAEWEQ-PVRRRRSRFSKHSESSG----------------------DSKHD----- 2759frog_Bassoon -------RTLAQSGQYWPP--VNQ-PFIAMAPTVPDAQG---QPRYPPPPRPLTNSASEMSLQTDDQWELNR-TIKKRNSMPRL-RDAYNADENHEPYI-VKKITDSSVQTDDEDGEDRFYMSRRRRTK-RSTDCSVQT-DDEDNADWEQ-PVRRRRSRFSRHSDSSS----------------------DSKHD----- 2756zebrafish_bassoon_a -------VISDQSGRIIQPQELASDVLYNDV---------------QVFVRSMPNSTSEICLRSTEDQAEIR-SMRKQRSMPRL-QDGNEGEVTMFPL--PQRLVDCSVQTDDEDGEERYLVSRRRRTR-RSVDCSVQTDDDEDKAEWEQ-PVRRRRSRYSRHSAESNT---------------------DHKSE----S 2728zebrafish_bassoon_b TTPQTISIIYDQSGRIIPQ--EGQSVQLLPCADGQVVQA-------VVSTRPLPSSSSEMSLKNSEEHGDR--IMKKQNSMPRL-RNGSEDES-------VKRIADCSVQTDDEDAEDKL-MSRRRRTR-RIADCSVQT-DEEDQGEWDNQPIRRRRSRYSKHSEPAA----------------------ETKSD----- 2463stickleback_bassoon_a -------IQLDANGQPLPP--------YVDS---------------QLGFRSLPNSSSEMCLRSQQEHVETRSNMRKHSSMTRLSRDSLDGDGVVFYGS-PRRIVDSSVQTDDEDGEERYMMRHRTRRRGRSVDCSVQTDDDEDKAETEH-PVRRRRSRFSRHAESSATGSSTAATSTTAS---------DSKTE----- 2930stickleback_bassoon_b -------CCSSRGSRRIGS----------------------------------GGSSSHYCPRSSDKQTDSK-MMKKQNSMPRL-RDGTEDDS-------LRKITDSCVQTDDEDGEDRF-MNRRRRTR-RIADSSVQT-DEEDQGEWEQQPVRRRRSRVSKHSESSG----------------------EAKSE----- 2634fugu_bassoon_a -------IQLDMNGQPLHP--------YMDS---------------QVGTRSLPNSSSEMCLRSQEDHMETRSNIRKHSSMTRL-----DGDGVMFYGS-SRRIVDSCVQTDDEDREERYMMRHRTRRRGRSVDCSVQTDDDEDKAVTEQ-PVRRRRSRFSRHSESTTTGSASAAT--------------DTKCD----- 2842fugu_bassoon_b GTPQSF-IICDQSGRVIQQ--DGQSVQFWQD--GQVVQA-------VVTARPIHSSVSEMSLRSSDKQADSK-AMKKQNSMPRL-RDGSEEES-------VKRITDSCVQTDDEDGEDRF-MGRRRRTR-RIADCSVQT-DEEDQGDWEQQPVRRRRSRVSKHSDSGG----------------------ETKAE----- 2498medaka_bassoon_b GAPQGF-IICDQSGRVLQQ--DGQSVQFWQD--GQVVQA-------VVAARPIQSSASEMSLRSSDKQTDSK-MMKKQNSMPRL-RDGLEEDS-------VKRIADSCVQTDDEDGEERF-MNRRRRTR-RIADCSVQT-DEEDQGEWDQHPVRRRRSRVSKHSEPGS----------------------EAKSE----- 2583medaka_bassoon_a -------IQLDVNGEPLHP--------YIDS---------------QMSSRSLPNSSSEMCLRTQEDHMEARASIRKHSSMSRLSRDNLDGDGVVFYGS-PRRIVDSCVQTDDEDGEERYMIRHRTRRRGRSVDCSVQTDDDEDKAESGH-PVRRRRSRFSRHSESSAAGSNSASTATSAT---------ETKSE----- 2809tilapia_bassoon_b GPPQGF-IICDQSGRVIQQ--DGQSVQFWQD--GQLVQA-------VVAARPIHSSASEMSLKSSDKQVDSK-IMKKQNSMPRL-RDGSEEDS-------VKRITDSCVQTDDEDGEERF-MNRRRRTR-RIADCSVQT-DEEDQAEWDQQPVRRRRSRASKHSESSG----------------------EAKSE----- 2586tilapia_bassoon_a -------MQLD--GQPLHP--------YADS---------------HMGSRSLPNSSSEMCLRSQEEQMEARTNMRKHSSMSRLTRDSLDGDGVVFYGS-PRRIVDSCVQTDDEDGEERYMMRHRTRRRGRSVDCSVQTDDDEDKAETEH-PVRRRRSRFSRHSESSAAGSSTATTATLAT---------DTKTD----- 2804cod_bassoon_a -------LQRDVTGQPLHP--------YVDA---------------QMASNSLPNSSSEMCLRSHEEQAESRSNMRKHSSMGRLPRDGGDAEGVSFYGSASRRNVDSCVQTDDEDGEERYMMRHRPRRRGRSVDCSVQTDDDEDKVEWEQ-PPRRRRSRFSRHSESNNHASGTAASTNTNSANAANATATDAKTD----- 2640cod_bassoon_b GPPQGF-IICDQSGRVIQQ--DGQSVQFWQD--GQVVQA-------VMAGRPIHSSSSEVSLRNTEDPASSR-VMKKQNSMPRL-RDGSEEE---------KRIADSCVQTDDEDGEDRF-LNRRRRTR-RIADCSVQT-DEEDQGEWDQQPVRRRRSRVSKHSDANV-----------------------EKSE----- 2513

:.:. ** : * *** ::* * **:** . **::::**:.**:* :*:**.***: : : : :. **: .* :.* . : . :::****::.:: : *: ****:**:*****: **:*:lizard_Bassoon -----SSSKGMASIAIQTISDCSVQTEPDQLHRVSPSIHITTPDP-KVEIVKYISAPEKTHRGESLACQTEPEVQPQPGIVVPQLTVPTTISPYS----SNLQIVSTGPLDPHSVR-------------------QQ-------VLAKKKPDPLEIGYQS--HLPTDSLSQLVSRQP---PKSPQVLYSPVSPL-SPHRL 2874mouse_Bassoon STTAAATARAMSSVGIQTISDCSVQTEPEQLPRVSPAIHITAATDPKVEIVRYISAPEKTGRGESLACQTEPDGQAQ-GVAGPQLIGPTAISPYL----PGIQIVTPGAL---------------------------------GRFEKKKPDPLEIGYQA--HLPPESLSQLVSRQP---PKSPQVLYSPVSPL-SPHRL 2815coelacanth_Bassoon ------SSKGTSSIATQTISDCSVQTEPDQLMRVSPAIHIITPDP-KVEIIRYISAPEKTQKGESLGCQTEPEAQSQ-GIAVPQLMVPTIVTPYS----TNIQIVTSGPLDPNASR-------------------QQ----GTSKFEKKKPDPLEIGYQS--HIASELM-----RQP---PKSPQVLYSPVSPL-SPHRL 2913frog_Bassoon -------AKGTSSIAIQTISDCSVQTEPDQLQRVSPSIHITTPDP-KVEIIKYISAPEKTQKGESLACQTEPETQSH-GIVAPQLSVPTTITPYS----SNLQTVPTGTLD--NTR-------------------QP----GLAKYEKKKPDPLEIGYQS--HLPADTLSQLVTRQP---PKSPQVLYSPVSPL-SPHRL 2912zebrafish_bassoon_a HFTPSSVKTASSSIAIQTIRDCSCQTETEQLVRVSPSIHATIPDPSKVEIVHYISGPERTQKGQSLACQTEPETQSQ-SIVIPQISIATTVSP------TGVHLVGSADPLSPSHQ-------------------------NAVKFGRRRPDPLEFGYQQH-HLHNESLSSLI-RQQ---PKSPQVLYSPVSPLSSPQRI 2891zebrafish_bassoon_b --------ASKSSIAIQTTNDSSCQTESDQLGRISPAIHITMAETSKVELLHYISAPERTHKGESLACQTDPEAQSH-GVVAPQLSVPTTVSPYS----TSLQLVGATPT----------------------------------KFERRKPDPLDIGYQQA-QQQNESPS----RQP---PKSPQVLYSPVSPL-SPHRM 2607stickleback_bassoon_a -----TPKMVSSSIAIQTIREMSCQTEVEHLGRVSPAIHVTVPDPNKVEIVHYISGPERTQKGQSLACQTDTEAQSQ-GVVAPQLSVPTTVSPYSSSTSTGTQQSGSSDLLNQQRQ-------------------QQHIAANAAKFERRRPDPLDINYQPHNHLHNESLSTII-RQHQTAPKSPQVLYSPVSPV-SPHRL 3103stickleback_bassoon_b ----GSSKVASASIAIQTTNDSSCQTEPDQLSRNSPAIHITMAETSKVDLLHYIAAPERTNKGESLACQTEPESQFH-GVVAPQLSVPTTINPYS----TSLHIVGANASEPTSPR-------------------HQ----GVVKFERRKPDPLEIGYQ-----QNESPS----RQP---PKSPQVLYSPVSPL-SPHRM 2789fugu_bassoon_a -----ALKMVSSSIGIQTSREMSCQTEVEHLGRVSPAIHVTMPDPNKVEIVHYISGPERTQKGQSLACQTDPEAQSQ-GVVAPQLSVPTTVSPYSSSTSSGSHQSSSVDLLTQQRQ-------------------QQNATANAAKFERRRPDPLDINYQPHNHLHNESISSII-RQQQSAPKSPHVLYSPVSPV-SPHRL 3015fugu_bassoon_b ----GTAKAASTSIAIQTTNDSSCQTEPDQLGRVSPAIHITVAETSKVDLLHYIAAPERTHKGESLACQTEPESQSQ-VVVAPQLSVPTTVSPYS----TSLHIVGANTADPTSLR-------------------LQ----GVAKFEKRKPDPLEIGYQ-----HNDSPS----RQP---PKSPQVLYSPVSPL-SPHRM 2653medaka_bassoon_b ----GSSKVASASIAIQTTNDSSCQTEPDQLGRVSPAIHITMAETSKVDLLHYIAAPERTHKGESLACQTEPESHSQ-GVVAPQLSVPTTISPYS----TSLHIVGANTPESASPR-------------------LQ----GVAKFERRKPDPLEIGYHQ---TQNEPTS----RHP---PKSPQVLYSPVSPL-SPHRM 2740medaka_bassoon_a -----APKMVSSSIAIQTIREMSCQTEVEHLGRVSPSIHVTMPDPNKVEIVHYISGPERTQKGQSLACQTDSEAQSQ-GVVAPQLSVPTTISPYSSSTSTGTQQSGSSDMLTQQRQ-------------------QQHISANAAKFERRRPDPLDINYQPHNHLHNESISSII-RQSQTAPKSPQVLHSPVSPV-SPHRL 2982tilapia_bassoon_b ----GSSKVVSASIAIQTTNDSSCQTEPDQLGRVSPAIHITVAETSKVDLLHYIAAPERTHKGESLACQTEPECHSQ-GVVVPQLSVPTTISPYS----TSLHIVGANTSDPISPR-------------------LQ----GVVKFERRKPDPLEIGYQQ---QQNESHS----RQP---PKSPQVLYSPVSPL-SPHRM 2743tilapia_bassoon_a -----TTKMVSSNIGVQTIREMSCQTEVEHLGRVSPAIHVTVPDPNKVEIVHYISGPERTQKGQSLACQTDPEAQSQ-GVVAPQLSVPTTVSPYSSSASTGTQQSGSDLL--TQQR-------------------QQQIAANAAKFERRRPDPLDINYQPHNHLHNESISSII-RQQQTAPKSPQVLYSPVSPV-SPHRL 2975cod_bassoon_a ----TSSKMASSSMSSQTMREMSCQTEVEHLGRVSPAIHVTLPDPNKVEIVHYISGPERTQKGQSLACQTDQEAQSQ-GVVAPQLSVPTTVSPYSSSTGNGSQQTGSSDLQAQQQRLQHHHQQQQQQLQQQQQQQQQQMAANVAKFERRRPDPLDINHQQHNHIHNESVSSVM-RQQQGTPKSPQVLYSPVSPV-SPHRL 2833cod_bassoon_b ------AKVSSSSIAIQTTFDSSCQTEPEQLGRVSPAIHITMAETSKVDLLHYIASPERTQKGESLACQTEPEAQSQ-GVVAPQLSVPTTISPYS----TSLHMVGANPSEPASPR-------------------LQ----GVAKFERRKPDPLEIGYQQ----QHES------RQP---PKSPQVLYSPVSPL-SPHRM 2665

:: :: : *:******:**:: :: . *: :***:*. ***:****.:***::: : ::. * *:::: . : **:*********:* : : ..: * .:* :*: **:**:****:****:**********: **:** :***: *: lizard_Bassoon LESSFTTSERLNKAHVTPQKH-FMADSTQRQHTLPRPIKTMQRSLSDPKPISPTSEEAAKDRFSLYQHTLLPGS-QISTL---QSNTLMRKVKRTLPSPPPEEA----HLPLAS-QAHSQMY-MPGLLQKGLGGQPVQV-TKASLLRELDRDLKLVEHESTKLRKKQAELDEEEKEIDAKLKYLELGIHHRKESLLKDRN 3062mouse_Bassoon LDTSFASSERLNKAHVSPQKQ-FIADSTLRQQTLPRPMKTLQRSLSDPKPLSPTAEESAKERFSLYQHQGGLGS-QVSAL---PPNGLVRKVKRTLPSPPPEEA----HLPLAG-QVPSQLY-AASLLQRGLAGPTTVPATKASLLRELDRDLRLVEHESTKLRKKQAELDEEEKEIDAKLKYLELGITQRKESLAKDRG 3004coelacanth_Bassoon LESSFTSTERLNKAHVTPQKH-FTTDLPQRQQTLPRPIKTMQRSMSDPKPISPTSEDSGKVRFPMYPQQHTLQS-QMSAL---PQSTLGRKVKRTLPSPPPEEA----HIPLAS-QPHPQMY-MSSLTQKGIPRAIPV--TKGSLLKEIDRDLKIVEQESTKLRKQQAELDEEEKEIDAKLRYLELGITQRKETLLKDRE 3100frog_Bassoon LESTFASSERLNKAHVTPQKH-FTADTTQRQQILPRPLKTMQRSLSDPKPISPTSEDSAKDRFSLYQHQLLQGS-QITAF---QPNTLTRKVKRTLPSPPPDEA----HLPLTTSQAHSQMY-MPNVTQKMMGGQAIQM-PKVGFLKTIDRDLKMVEQESTKLRKQQAELEEEEKEIDAKLKYLELGITQRKESIVKDVG 3101zebrafish_bassoon_a IDTSFSSSERLNKAHVTPQQKSCIAEPSQRHQAIPRPMKNIQRSMSDPKPLSPTTDEHTKARISLYQQQALQS--QLAAL---QQSSLLRKVKRTLPSPPPEET----QMPMVT-HNLSHML-VPSLPSLK-GGSRLA--AKTSLLKDLTHELKAVEQESTKLRKQQAELEEEEKEIDAKLRYLELGITQRKETLAKERD 3077zebrafish_bassoon_b LETTFASSEKLNKAHVTPQQKAFTTESPQRHQTIPRPIKSVQRSMSDPKPLSPTSEDPAKTKFSLYQSQASPAS-QMSAL--QQQNAMMRKVKRTLPSPPPDES----PLPIMT-PAIPHIYSSPGVSQRVLPRTAQGA-MKAGLLS----ELKAVEQESTKLRKQQAELEEEEKEIDAKLRCLEMGITQRKETRVKERE 2794stickleback_bassoon_a LENSL-SSERLNKAHVTPQQKSYTAESPQRHPSVPRPIKSTQRSMSDPKSLSPTTDEHTRARLSLYQQQVLQS--QLAAL---QQSSLLRKVKRTLPSPPPDENTTSAHLPMMA-PSLQQVY-LPSVPSLK-PSSRSGLAAKASLLKDLTHELKAVEQESTKLRKQQAELEEEEKEIDAKLRYLELGIHQRKETLVKERE 3294stickleback_bassoon_b LETTFASQEKLNKAHVTPQQKAFTTESPQRHQTLPRPIKSVQRSMSDPKPLSPTSEDPAKNRFSPYHQQAVSNSQQMASL-QQQQNALMRKIKRTLPSPPPEET----SLPIVT-PA--QMYSSPGMPQRVLPRPAQGV-TKAGLLS----ELKAVEQESSKLRKQQAELEEEEKEIDAKLRYLELGITQRKETMVKDRE 2976fugu_bassoon_a LETSL-SSERLNKAHVTPQQKSYTAESPQRHPSMPRPIKSSQRSMSDPKSLSPTADEHTKARLALYQQQALQS--QLAAL---QQSSLLRKVKRTLPSPPPDETTTSAHLQ--------QLY-MPSVPSLK-PSSRSGLAAKASLLKDLTHELKAVEQESTKLRKQQAELEEEEKEIDAKLRYLELGIHQRKETLVKERE 3199fugu_bassoon_b LETTFASQEKLNKAHVTPQQKAFNAESPQRHQTLPRPIKSVQRSLSDPKPLSPTSEDPGKNRFSPYHQQALSNS-QMASL--QQQNSLMRKVKRTLPSPPPEET----PLPIVT-PA--QMYSSPGMSHRVLPRHVQGV-TKTGLLS----ELKAVEQESSKLRKQQAELEEEEKEIDAKLRYLELGITQRKETMVKEKE 2838medaka_bassoon_b LETTFTSQEKLNKAHVTPQQKAFTAESPQRHQSLPRPIKSVQRSMSDPKPLSPTSEDPAKNRFSPYHQQAVANS-QMTSL-QQHQNSLMRKIKRTLPSPPPEES----PIPIVT-PA--QMYSSPGITQRIHPRPAHAV-TKTGLLS----ELKAVEQESTKLRKQQAELEEEEKEIDAKLRCLELGITQRKETMVKDRE 2926medaka_bassoon_a LEATL-SSERLNKAHVTPQQKSYTAESPQRHPSMPRPIKSGQRSMSDPKSLSPTADEHTKARLSLYQQQALQS--QLAAL---QQSSLLRKVKRTLPSPPPDETAPSAHMPMMT-PVLQQAY-LPSMPSLK-PSSRSGLAAKASLLKDLTHELKAVEQESTKLRKQQAELEEEEKEIDAKLRYLELGIHQRKETLVKERE 3173tilapia_bassoon_b LETTFASQEKLNKAHVTPQQKAFTAESPQRHQTLPRPIKSVQRSMSDPKPLSPTSEDPVKTRFSPYHQQAVSNSQQMASL-QQQQNSLMRKVKRTLPSPPPEET----PLPIVT-PA--QMYSSPGMHQRVLPRPAQGV-TKAGLLS----ELKAVEQESSKLRKQQAELEEEEKEIDAKLRYLELGITQRKETMVKERE 2930tilapia_bassoon_a IETSL-SSERLNKAHVTPQQKSYTAESPQRHPSVPRPIKSTQRSMSDPKPLSPTTDEHTKARLSLYQQQALQN--QLAAL---QQSSLLRKVKRTLPSPPPDETATSAHLPMMT-PALQQVY-LPSVPSLK-PSSRSGLAAKASLLKDLTHELKAVEQESTKLRKQQAELEEEEKEIDAKLRYLELGIHQRKETLVKERE 3166cod_bassoon_a LESSL-SNERLNKAHVTPQQKSYTAESPQRHPSVPRPIKTTQRSMSDPKSLSPTTDEHTKARLSLYQQQALQS--QLAAL---QQSSLLRKVKRTLPSPPPEEATAAVHLPMMT-NAMPQVY-MPNVPGLKQPCSRSGLAAKASLLKDLTHELKAVEQESTKLRKQQAELEEEEKEIDAKLRYLELGIHQRKETLVKERE 3025cod_bassoon_b LETTFASQEKLNKAHVTPQQKAFTTESPQRHQSLPRPIKSVQRSMSDPKPLSPTSEDPGKNRFSPYHQQALSNS-QMASLQQQQQNSLMRKVKRTLPSPPPEEI----PLPIVT-PA--QMYSSPGMPQRVLPRPAQGV-TKAGLLS----ELKMVEQESSKLRKQQAELEEEEKEIDAKLRYLELGITQRKETMVKDRE 2852

*: . :* :*: ***:*****.::*: *:* :: * . :. : : * : * . . .* . :. lizard_Bassoon VRDYPYLRCLGENRDYMSDSELNNLRL-----SGYESTSLL-TRPSTAPSNQYAEF--SSSQYTSASSYTP--YQYSSGHAAPPSAAT---FQQTRFQP--PHYPPASTG---PAQNGFQAS---QMPAYPSQTTYQTHSFTPSTG-Y-QSELGLQS-----------HQAFR----PPYQSPTT-----YPSQTPMQP- 3218mouse_Bassoon GRDYPPLRGLGEHRDYLSDSELNQLRL-----QG-----------CTTPAGQYVD------------------YPASAAVPATPSGPTA--FQQPRFPPAAPQYTAGSSG---PTQNGFPAH---QAPTYTGPSTYPAPTYPPGTG-Y-PAEPGLPS-----------QPAFH----PTGH---------YAAPTPMPT- 3135coelacanth_Bassoon RRDLSYIRCLGDNRDYMSDSELSNIRI-----SAYEGNGIL-ARPSTAPINQYSDF--STAQFPSTSSYAP--YQYTAGQAVPQGPTN---YQHGGFQA--PHYSVSSSG---QSQNGFQAQ----PSTYHAQSNYQPHGLPQTSN-Y-ESEHSLQS-----------HTAFQ----PQN--MYQAQTT-YPNQNTFQP- 3257frog_Bassoon RRDFPYMRCLGENRDYMSDSELNNVRI-----SGYDTNGLS-NRMGTAP--QYSDFQTTQLHQASTSSYTPSTYQYTTGQPVTPAATS---LQQTRFQQ--PQHPVSTNG---PSQSTFQNQ----LSGYPAQSSYQTHNIVQNTS-Y-QSGPGQQN-----------HTGFR----PPN--PYQTQTT-YANQQPLQT- 3260zebrafish_bassoon_a RRELAYLRCMGDSRDYMSDSELNNLRLSAAA-SSFESNGLM-TRPNTAPLSQFTCELNTAAQFPPTSSFVS--YQYPQSLPTVPTPQAST-LQSTSFNQ--PPYPSVTQAQALPQPTPLQTY---PTLPYQVQGSFPSQVFPQSQPPY-PADTSMPPSAQPS------QPGFQATLPPTGPVPYPTHNTPYPSQAPPYPV 3260zebrafish_bassoon_b RRELAYLRCIGDARDYMSDSELNNLRLAGVAPGTYEENGML-SRPSTAPLSQFTSDLN-ASQYPPTSSYVP--YQYPQPQPTPTQPPTA--TYQIGFQP--APYPTSSQ----PQPGTFQPH-PPQPSSYQTPGPYQTHNYGQPT--Y-ESDLGLQQPS---------HQGFQ----PPTA-PLAGQTLPYPAQS----- 2959stickleback_bassoon_a RRDIAYLRCMGDTRDYMSDSELNNLRLAAAA-ASHETNGLLSTRPSTAPLSHFASDLNTAAQYPPSSSFIS--CQYPQSQQAAPMPQSNVPYQASTFNQ--PPYPSVSQSQAVPQPTPLQSHVPPLGPSYPSQTSYPSHTYPQTGPPYPQTDIGLPAAPQPQPQPQPVHTGFGPA--PPGQPPYPTHSSPYPSAVSSYP- 3486stickleback_bassoon_b RRELAYMRCMGDARDYMSDSELNNLRMVAAT-GTFDANSLL-TRPSTAPLSQFANDLNVTSQYPSTSSYMS--YPYPQVQPSTQQPGSA--YQQIGFQP--PQYPSSSA----PQPGTFQPH-PPPGPGYQNQGTYSNRMYAQPS--Y-QTDLGMQQHG---------NQGFH----PSGQ-PMPSQNLPYPSHSSYQP- 3145fugu_bassoon_a RRDIAYLRCMGDTRDYMSDSELNNLRLAAAA-ASHDANGLLSSRPSTAPLSQFTSDLSTGSQYPPSSSYLS--SQYSQSQPTAPLQQTSVPYQSSAFNQ--PPYPSVSQSQALPQPTPLQSHVPPPGPSYQPQTSYPSHSYPQTQPPYPQTDIGLPAAPQPQPQPG--HTGFGPA--PPGQPPYPTHSSPYPSAVSSYP- 3389fugu_bassoon_b RRELAYLRCIGDARDYMSDSELNNLRMAGVT-GNFDSNGLL-TRPSTAPMSQFTNDLNAAGQYPSTSTYIT--YPYSQSQPSTQQPSSG--YQPIGFQP--PQYPSTSA----PQPGTFQPQ-PPPGPAYQNQGAYSNRMYDQSS--Y-QTDHGMQQHG---------HQGFH----PPGQ-PTTIQTIPYPSHSSYQP- 3007medaka_bassoon_b RRELAYLRCMGDARDYMSDSELNNLRMGA---GTFDANGLL-TRPSTAPLSQFTNDITSTSQYPSTSSYLP--YPYPQSQPSAPQQSS---YQQIGFQS--PQYSSSSA----PQPGTFQPH-PPPGPGYQNQT-YSSRMYTQSS--Y-QTDIGMQQHG---------HQGFH----PPAQ-PMPAQTLPYPTHSSYQL- 3091medaka_bassoon_a RRDIAYLRCMGDTRDYMSDSELNNLRLAAAA-ASHETNGLVTTRPSTAPLSQFSSELSMGGQYPPTSSFLS--SQYPQSQPAAPSQQSSAPYQSSTFNQ--PPYPTVSQSQALPQPTPLQSHAPPPGPSYQSQTSYPSHTYPQTQPPYPQTDLGIPAAPQPQPG----PTGFGPA--PPGQPPYPTHSSPYPSAVSSYP- 3361tilapia_bassoon_b RRELAYLRCMGDARDYMSDSELNNLRMAAAT-GTFDANGLL-TRPSTAPLSQFTNDLTATSQYPSTSSYMS--YPYPQT--STQQPGST--YQQIGFQP--PQYPSSSA----PQPGTFQPH-PPPGPGYQNQGTYSSRMYGQSS--Y-QTDIGMQQHG---------HQGFH----SPAQ-PMPGQSLPYPTHSSYQP- 3097tilapia_bassoon_a RRDIAYLRCMGDTRDYMSDSELNNLRLAAAA-ASHESNGLLTTRPSTAPLSQFTSDLNTAAQYPPTSSFLS--CQYPQSQPAAPSQQPSVPYQSATFNQ--PPYPTVSQSQALPQPTPLQTHAPPPGPTYQPQTSYPSHTYPQTQPPYPQTDMGLPAAPQPQPQPG--HTGFGPA--PPGQPPYPTHSSPYPSAVSSYP- 3356cod_bassoon_a RRDIAYLRCMGDSRDYMSDSELNNLRLAAAA-ASFETNGLLAARPSTAPLSQFTGELNTAAQYPPTTSFLT--YQFQQGPSAASSQQTSAPYPTASFNQ--PPYPSVSQSQALPQPTPLPSHGPMPAPSYPSQTPYPSQTYPQAQPPYPQTDIGVPAAPLSQPQAA--HPGFGPA--PPGQPPYPTHSSPFPSAVSSYQ- 3215cod_bassoon_b RRDLAYLRCMGDARDYMSDSELNNLRIAAAT-GNFDTNGVL-SRPSTAPLSHFANDLNATQQYPSTSTYMS--YPYPQTQPSAQPPGSA--YQQIGFQP--PQYPSSSA----PQPGTFQPH-PPPGPAYQTPGSYSAHMYAQSS--Y-QPDLGMPQHG---------HQGFQ----PPCQ-PMPGQSLPYPSHSSYQP- 3021

: : : :****:*****:*:.****.* .*:: :::: : : . .:: . lizard_Bassoon AQSSLY--QTHTDTHTT-SQKPRQTSLADLEQKIPTNYEVISNPTVVISAATPEVTYS-------------TTTVTSSYDQYKAPE-VRPADRS--------------------------------------------------------------GAAQSPSTSYSSDSLY--TNLEQNIPRNYVMIDDISELTKESTA 3337mouse_Bassoon TQSAPF--PVQADSRAA-HQKPRQTSLADLEQKVPTNYEVIGSPAVTMSSAPPETGYS-------------GPAVSGSYEQGKAPEHPRGSDRS--------------------------------------------------------------SVSQSPAPTYPSDSHY--TSLEQNVPRNYVMIDDISELT-KDST 3254coelacanth_Bassoon VQNAPF--QSQADILAV-HKKPRQTSLADLEQKIPTNYEVIANPTVVISSTTAEVTYT-------------TTTVTSSYDQYKNSE-PRMADRS--------------------------------------------------------------NIVESPSANYSSESLY--TNLEQNIPRNYVMIDDISELT-KENS 3375frog_Bassoon PINSGF--QTQ-DSHTT-HQKPRQTTLADLEHKVPTNYEVIVNPTVVISSATPDSAFS-------------STTAASSFDQYRAPD-VRPTERG--------------------------------------------------------------SGAESPSNSYPSDSLY--TNLEQNIPRNYVMIDDISELT-KQNP 3377zebrafish_bassoon_a SQASTY--QPQTDTLTF-HQKPRQTSLADLEHKMPTNYEVISNPTVVVTTTAQEISYSQSGLGPSYGQY--STTMASTYGPYSST--VSNTYGGPLTTSAPSGYGPYTTISSSSYGQYTTTTANAYGYTVTTASSYGQYNPVVTNSYGQTTLSDHMHSMDSPSM-YGSDGLYGSSNLEQSVPRNYVMIDDICELT-KDGM 3451zebrafish_bassoon_b ---MSF--QPQGDMLSA-HQRPRQTSLADLEQKLPTNYEVISNPSIPVATSAQDTGFS-----GVY-----SSPLNNAYGQYRPSDQPLT------------------------------------------------------------------ERIPSPSSGYGTDGVY-ATNLEQNIPRNYVMIDDISELT-KENM 3075stickleback_bassoon_a GQTTPYPGQPQADILTVHQGRPRQTSLADLEHKMPTNYETISNPTVVVTTTAQDCNYS--SAAPSYGQYTGMTTMASSYGPYGSA--PVSSSYGGYSTMPPSTYGQYTSSSANSYGQYTTTTANTYGYTTTTASSYGQYTSTVSNVYG--------HSVDSPSIYSSSDGMYGAPGLEQNIPRNYMMIDDVSELTAKDGL 3674stickleback_bassoon_b --ALSY--QPQADILTV-HQRPRQTSLADLEQKLPTNYEVISNPAVSVATSVPDSNFG--------------SVYSNAYGQYRPPE-HGLP-----------------------------------------------------------------HSVESPPSAYSSDGVY-ASNLEQNIPRNYVMIDDISELT-KDNA 3258fugu_bassoon_a SQTTPYPGQPQADILTVHPGRPRQTSLADLEHKMPTNYETISNPTVVVTTTAQDATYS--STAPTYGQYTGSTTMASSYGPYGSA--PVSSSYGGYSTMPASTYGQYTSNAASSYAPYTTTIANTYGYTTTTASSYGQYSSTVSNAYG--------HSVDSPSNYGTAEGMYGAPGLEQNIPRNYMMIDDVSELTAKDGL 3577fugu_bassoon_b ---GLY--QPQAEILTVHHQRPRQTSLADLEQKVPTNYEVISNPAVSVATSAPDANYG--------------AIYNNAYGPYRPPE-PGLT-----------------------------------------------------------------HTMESPTSAYASDGLY-TSNLEQNIPRNYVMIDDISELT-KDNA 3120medaka_bassoon_b --GLTY--QPQAEILTV-HQRPRQTSLADLEQKIPTNYEVINNPAVSVATSAPDPNFG--------------PTYSNAYGQYRPPE-PGLT-----------------------------------------------------------------HSVESPTSAYPSDGLY-TSNLEQNIPRNYVMIDDISELT-KDNT 3204medaka_bassoon_a SQTAPYPGQPQADILTVHPGRPRQTSLADLEHKMATNYETISNPTVVVTTTAQDATFS--SAPPSYGQYTGTTTMASSYGPYGSA--PVSSSYNGYSTMPPSTYGPYTSASVSTYGLYTTTNANTYGYTTTTASSYGQYPSTVSSAYG--------HPVDSPSTYSTANGMYGAPGLEQNIPRNYMMIDDVSELTAKDGL 3549tilapia_bassoon_b --GLSY--QPQAEILTV-HQRPRQTSLADLEQKLPTNYEVISNPAVSVATSAPDSSYG--------------SAYGNAYGQYRPPE-PGLT-----------------------------------------------------------------HSVESPTSAYATDGLY-TSNLEQSIPRNYVMIDDISELT-KDSG 3210tilapia_bassoon_a SQTTPYPGQPQADILTVHPGRPRQTSLADLEHKMPTNYETISNPTVVVTTTAQDATYS--SAAPSYAQYTGATTMASSYGPYGSA--PVSSSYGGYSTMPPSTYGQYTSTTASSYGQYTTTTANTYGYTTTTASSYGQYTSTVSNAYG--------HSVDSPSTYSTADGMYGAPGLEQNIPRNYMMIDDVSELTAKDGL 3544cod_bassoon_a SQSTPYPGQQGADILTVHPGRPRQTSLADLEHKMPTNYETISNPTVVVTTTAQDVTYS-SAAAAAYAQYGATTTMAGSYGGYPAA--PAVN------------------------------------------------------------------------------------------------------------- 3303cod_bassoon_b --VLPF--QSQADILTV-HQRPRQTSLADLEQKLPTNYEVISNPAVSVATSAPDTGFS--------------AVYSNTYGQYRPPE-PGLT-----------------------------------------------------------------QPVESPTSAYAHDGLY-TTNLEQNIPRNYVMIDDISELT-KENA 3134

:. : . :: . :. : * : .:.* .*. *:**.**:* . :***:*********:***:*: *:*. * lizard_Bassoon GES---HKGDLQSQS----SNGRHIKEKSDLTDADI-SSRPCC----YSKGEEESEEDMYDHLSSDHRGKS-YH-RGTENNG------RMSGSSGSYYYGDSDY-RHPSRQDKHGSSMGIQKHSSKNL-APAVISSKRSKHRKQG-MEQKISKFSPIEEAKDVESDL-ASYAVTTSVSSSN--------VASRAKKLQDD 3505mouse_Bassoon PTASESQRLEPLGPG---GVSGRPGKDPGEPAVLEG-PTLPCC----YGRGEEESEEDSY-----DPRGKSGHH-RSMESNGRP----------STHYYGDSDY-RHGARADKYGPGPMGPKHPSKSL-APAAISSKRSKHRKQG-MEQKISKFSPIEEAKDVESDL-ASYPPPTVSSS----------LTSRGRKFQDE 3416coelacanth_Bassoon PPV-ESQKIDQQPPC----TNGRHLKERTEVTE-NGNSSRVNS----YSKTGEESEEDAYDYHVSDHRGKNSYH-RSSNNHGRMT---NCSSSGSSYYYADNDFNKHSSRSEKHGSSLGMPKHSSKNL-APAVTSSKRSKHRKQS-MEQKISKFSPIEEARDVESDL-ASYTVTTSANSSS--------VISRGKKLQED 3550frog_Bassoon PTT-ENQKVEALSQSLPQTSNGRHIKDKNGHHDIDN-SSKPCC----YSRTEEESEEDVYDHHSSDYRNKNSYQ-RGPDNN-------GRDSGSGSYYYEN-DS-RHSTRLDRHGPSTVMQKHSSKNL-APAVVSSKRSKHRKQG-MEQKISKFSPIEEAKDVESDLAASYPVTTSVGSSNI-------VSSRAKKLQDE 3552zebrafish_bassoon_a GTSSDSHRGE---------GHGRYGSD-------GL-HTRGQSS---FGRPEDERDADLYDQHHGRGKSTCSYQAQGANTHGRVVG--SSSMGGGSSYYYD-DC-SHS------APTWAAQRHSTKNLCPSAVMSSKRSKHRKQG-IEQKMSKFSPIEEAHDVEADL-ASYTMGTSATSGYG-------SGS------QY 3606zebrafish_bassoon_b GSS------DMARLG--------YGSE-------NG--PRGPT----YG----DGSENLY------GRTSNTYQ-GGVDSHGHP----ITSGSGGSGYYYD-DY-KHS---SRSSGGMGSQKHSSKNL-APAV-SSKRSKHRKQG-MEQKISKFSPIEEARDVESDL-ASYTMTTSTGGSCT-------VVSRT------ 3211stickleback_bassoon_a GTT----TGDMMHHG----GSGRYPGDIHGHITTVGSSTRGGTGNS-YGRAPEEEAAMQEELYDHHGRGKSSYR-HGPLGGSSSSV--SSSMGGGSSYHSDYDY-KHG--SIRS--GVQKPMSSTRSLLAPAVMSSKRNKHRKLGSMEQKISKFSPIEEARDVEADL-ASYSSTTGGAYPS--------SHIRGRQLIED 3848stickleback_bassoon_b SQS-----TDAQGHP----VGGRYRNE-------NG-PARGSA----YSR-PEDEPVDGY----GRPSGTTGYQ-NTVDSRP------STTVSGGSSYYYD-DY-KHP---SRS--GSSSHKLAPKNL-APAVVSSKRSKHRKQG-MEQKISKFSPIEEARDVESDL-ASYTMTTSTGGSCT-------VVSRSKKLQDD 3408fugu_bassoon_a GTT----TGDMMHHG----SSGRYPGDIHGHSSTIGSSTRGGTSSSSYGRAPEEEAAMQEELYDHHGRGKSSYR-HGPLGGSSSSV--STSLGGGYPYYYDYDY-KYG--SIW----VQKPSSSSRSLLAPAVMSTKRSKHKKLGNMEQKISKFSPIEEARDVETDL-AAYSSATGGVYPS--------SHIRGRQLIED 3750fugu_bassoon_b NQP-----TDALGQP----LGGRYRSE-------NG-PLRGNA----YGR-ADDEPVDAY----GRPTSTGGYH-STTDSRT------TPTVSGASTYYYD-DY-RHT---SRS--SSSSHKLAPKNL-GPAVVSSKRSKHRKQG-MEQKISKFSPIEEARDVESDF-ASYTMTTSTGGSCT-------VVSRAKKLQDD 3270medaka_bassoon_b GQP-----TDALGHP----TGGRYRNE-------NG-PARGT-----YGR-PEDGPLDAY----GRPMSSAGYQ-STVDTRT------STTVSGGSPYYYD-DY-KHS---TRS--G--SHKPTSKNL-APAVVSSKRSKHRKQG-MEQKISKFSPIEEARDVESDL-AAYPMATSSGSTGA-------VGSRSKRHQDD 3351medaka_bassoon_a GTT----TGDMMHHG----GGGRYQGDIHGHSSTTGSTARGGTGSSPYGRAPEEETAMQDELYDHHGRGKSSYR-HGPLGGSSSV---STSMGGGFPFYYDYDG-KHG--SIRS--GVQK-NVFSRSLLAPAVMSSKRSKHRKLGSMEQKISKFSPIEEARDVETDL-ASYSSTTGGAYPS--------SNIRGRQLMED 3722tilapia_bassoon_b QPT------DALGHP----VGGRYRSE-------NG-PARGSA----YSR-PDDEPVDAY----GKPISSTGYQ-TTVDSRT------GTTVSGGSSYYYD-DY-KHS---SRS--SSSSHKLTSKNL-APAVVSSKRSKHRKQG-MEQKISKFSPIEEARDVESDL-ASYTMTTSTGGSCT-------VVSRSKKLQDD 3359tilapia_bassoon_a GTM----TGDMMHHG----GSGRYPGDIHSHSSTTG-TTRGGTGSSPYGRAPEEEAAMQEELYDHHGRGKSSYR-HGPLGGSSS----SASMGGGSPYFYDYDY-KHG--SIRS--GVQKPSPSSRTLLAPAVMSSKRSKHRKLGSMEQKISKFSPIEEARDVEADL-ASYSSVTGAAYPS--------SHIRGRQLIED 3716cod_bassoon_a -------------------------------------GTGGSS----YGRTGEEEAAFQEELYDHHGRGKSSYR-QRPLGGSSSSSSVSASMGGGSPYYYDYDH-KHPT-TPRP--GAQKPSASARSLLAPAVMSSKRSKHRKSGSLEQKISKFSPIEEARDVEADL-ASYASATVGGHGSSSSAATAAAGVRGRQLIED 3456cod_bassoon_b GQA-----TDAHGHP----AGGRYGGE-------NG-SSRG------YGR-PEDESVDAY----GRPAGTAGYQ--GVDGHA------GSTVSGGSSYYYD-DY-KHP--NTRC--SSGTQKASSKNL-APAVVSSKRSKHRKQG-MEQKVSKFSPIEEARDVESDL-ASYTMTTSTGGSCT-------VVSRSKKQDDG 3282

: . * :: :::* * . .*** . : :* * : *.:.* **: : . : * *::*.. .: . ****:****: * : *: : ** :: *: ** *.*****. *.* lizard_Bassoon ITYGLKKNVYEQ-------QKYYSVSSRDMMEEDDRA-Y-SGGRSRSSG-YVSDKMSSREI---RSRSY--ERESMERPQKGSSKPSSLSMSQSRGRPPIRTQTSEEESPISPLGKSMGVSRSSG-GPLPQSAGDS-----CSQFCSSHSLPDVQE-HIKDVPRTHSYKHEEGYLMDDSHCVVSDSEAYHLGQEETDWFD 3683mouse_Bassoon ITYGLKKNVYEQ-------QRYYGVSSRDAAEEDERM-YGSSSRSRMASAYSGEKLSSHDYSS-RGKGYERERDTAERLQKAGSKPSSLSMAHGRARPPMRSQASEEESPVSPLGRP----RPAG-GALP--PGDT-----CPQFCSSHSMPDVQE-HVKDGPRAHAYKREEGYMLDDSHCVVSDSEAYHLGQEETDWFD 3594coelacanth_Bassoon ITYGLKKNVYDQ-------QKYYGISGRDVTEEDDRI-YSGSSRSRSSSGYQMEKSSSRDTINSRSRSY--ERETVERSQKGSSKPSALGMSQNRGRAPMRTQTSEEESPLSPLGKPVDMPRASG-GPLPPAPIDS-----HAQFGSSHSLPDVQE-HVKDVPRNHAYKPEEGYIMDDTHCVVSDSEAYHLGQEETDWFD 3733frog_Bassoon ITYGLKKNVYEQ-------QKYYGVSNRDMVEEDDRI-YGSGSRSRSSSSYGLEK-SNRDSGSSRSKSY--ERDSSDRYHKSSSKPSTLSMNQGRGRAPMRTQASEEESPVSPLGKSVGVSRSSG-GHVSQSTVDS-----CPQFCSSHSLPDVQE-HIKEVPRNHTYKQEENYMMDDSHCVVSDSEAYHLGQEETDWFD 3734zebrafish_bassoon_a --YKLQDGIYALRSTYSPQRGYY---------NDERL-Y-GYGRSRSAG-YGMDKISPCE-RSYRSRSY--ERDRVDRSYHSN--------YGHSVHPTLQSQYSDKENS-HAMTKLMGVERAFG-YPSN-------------SYGSSHSLPDVQD-HIRDLPRTHVYKPDDMYIIDDMHCAVSDSEAYHLGQEETDWFE 3765zebrafish_bassoon_b ---GVKKG-YDQ-------QKYYG--SREALEEDDRL-Y-GSGRSRSTG-YGMDKISSRDSSGYRSKSY--ERDAMERSQRGT--------HGRSGRPSMRTQNSEEESPLSPVGKPMGIGR----GSVPDDVRN--------QYGSSHSLPDVQDHHMRDIPKSHVYKPDDPYLADDMHCAVSDSEAYHLGQEETDWFE 3373stickleback_bassoon_a --YGFKSNTYEGGSGSTHGSRHYG----STVEEDDRIYYNSTGRSRSTG-YGMDKISARDYSGYRSRSY--ERD--DRSYRSG---------YSRGRNPTR-QYSEEESPLSPLRRFMGSGRSSSLGPDPYDSRSGRYHYYYGQYGSSHSLPDVQD-HIQDLPRTHVYKSDDTYIIDDYHCAVSDSEAYHLGQEETDWFE 4026stickleback_bassoon_b ATYGIKKNTYDQ-------QKYYG-TSRDGLEEEDRM-Y-SSGRSRSTG-YGMDKISSRDATGHRSKSY--ERDAMERSQRSS----------RSGRPPMRSQNSEEESPISPVGKPVGMGGGSG-IPEAHDVRN--------QYGSSHSLPDVQDHHKKDQPRSQVYKPDDPYLVDDMHCAVSDSEAYHLGQEETDWFE 3576fugu_bassoon_a --YGFKRSAYDS-PATTHGSRYYG---GSVVDEDDRIYYTSTGRSRSTG-YGMDKISARDYPGYRSRSY--ERD--DRSYRSG---------YNRGRQPTR-QYSEEESSLSPLGRFRGSGRPSSLGPHPYDNRSSRY-YYYGQYGSSHSLPDVQD-HIRDLPRTHVYKSDDTYIIDDYHCAVSDSEAYHLGQEETDWFE 3927fugu_bassoon_b VNYGMKKNAYDQ-------QKYFG-SNREGLEEEDRM-Y-SSGRSRSTG-HGIDKISSRDTSGHRSKSY--ERDAMERCQRST----------RSGRPPMRNQNSEEESPLSPVGNTVGMGRRSG-IPDSHDVRN--------QYGSSHSLPDVQDHHKKDMPRSHVYKPDDPYLIDDMQCAVSDSEAYHLGQEETDWFE 3438medaka_bassoon_b MTYGMKRNVYDQ-------QKYYG-TSRDRLEEEDRM-Y-SSGRSRSTG-YGMDKISSRDATGHRSKSY--ERDAMERSQRSS----------RSGGPPMRNQNSEEESPLSPVGKPVGMGRGSV-ISEAHDVRN--------QYGSSHSLPDVQDHHKKDLPRSHVYKPDDPYLVDDMHCAVSDSEAYHLSQEETDWFE 3519medaka_bassoon_a --YGFKRSAYEGGSGTAHGSRYYG----MMVDDEDRIYYTSTGRSRSHG-YGMDKISAKDYHGHRSRSY--ERD--DRSYSAG----------HRGRHPTR-QYSEEESPISPLGRLMSSGRSSSLGPDPYDSRSSRHPYYYGQYGSSHSLPDVQD-HIGDLPRTHVYKSDDTYMIDDNYCAVSDSEAYHLGQEETDWFE 3899tilapia_bassoon_b ATYGMKKSAYDQ-------QKYYG-TSREGLEDEDRM-Y-SSGRSRSTG-YGMDKISSRDATGHRSKSY--ERDAMERSQRSS----------RSGRPPMR-QNSEEESPLSPVGKPVGMGRSSG-ISEAHDVRN--------QYGSSHSLPDVQDHHKKDLPRSHVYKPDDPYLVDDMHCAVSDSEAYHLGQEETDWFE 3526tilapia_bassoon_a --YGFKRSAYDSGSGTTHGSRYYG----MTVDEDDRIYYTSTGRSRSTG-YGMDKISARDYSGYRSRSY--ERD--DRSYRSG----------YRGRHPTR-QYSEEESPLSPLGRFMGSGRSSSLGPDPYDSRSGRYKYYYGHYGSSHSLPDVQD-HIRDLPRTHVYKSDDTYFIDDYHCAVSDSEAYHLGQEETDWFE 3893cod_bassoon_a --YGFKCNTYDSGSATGHASRYY----RSKAEEDDRLYYTSTGRSRSTG-YGMDKISSGDYSGYRSRSY--ERD--DMSYRTGG-----GGGYTRGRHPTR-QYSEEESPISPMGKFMGSGRSSSLGPDPYDSRSSRYHYYYGQSGSSHSLPDVQD-HIRDLPRTHVYKPEDMYLIDDYHCAVSDSEGVPLGQ------- 3631cod_bassoon_b --YGPKKNTYDQ-------QKYYG-TSREGLEEEDRM-Y-GSGRSRSTG-YGMDKISSRDSTGYRSKSY--ERDAMERSQRTG----------RGGRPPMRSQNSEEESPLSPVGKPVGIGRGSG-APDANDVRN--------QYGSSHSLPDVQDHHMKDQPRSHVYKPDDPYLVDDMHCAVSDSEAYHLGQEETDWFE 3448

. .:. : : * lizard_Bassoon KPREPRSERMRHYGGHS-SSSQKRGPVKHTYHDYDEPPDEDMWHHDDYS-HP---RHSSSKEHRHHGDY--GRHSSSRHSSDDL-PRRSSRQHPRDP-GRHESRGHGQPSSQKKAQQADMRAQPGYPSSSSEYSQQTRQP-SGYHHTSESQKSQR--------------------QHGPPPSHQPKSE----SLSHQQQQ 3849mouse_Bassoon KPRDARSDRFRHHGGHTVSSSQKRGPARHSYHDYDEPPEEGLWPHDEGG-PG---RHTSAKEHRHHSDH--GRH-SGRHAGEEP-GRRAAKPHARDM-GRHEARPHPQASPAPA---MQKKGQPGYP-SSADYSQSSRAP-SAYHHASESKKGSR--------------------QAHTGPSALQPKA----DTQAQPQM 3756coelacanth_Bassoon KPRELRSERSRHSSGHS-SSSQRRNHIKHTYHDYDEPPDEDLWQQDDYS-QS---RHSSSREHKHHGES--GRH-SSRHTSDEL-PRKSSKQHPKDQ-GRHESRQHSQQPAQKKTHQSEQRTQQSYPVSSSEYSQQSRQP-SGHHHASEAQRTQKQTQQVQLQQQQQQSSSKKPEPHS----QQQPRQ----QHTGQQQP 3914frog_Bassoon KPREARPDRSRYYSSH--SSTQKKIPVKHTYHDYDEPPDEDTWSQDDYS-QS---RHSSSKDHRHHGES--SRHSSSRHPEEQP--RRSSKQHPRDQ-GRHESRPHSQPSSSSK--KSDTRGQSGYPPSSSDYVSQSRQQ-SSYHHSSESQKSVR---------QSQMSQQQKPDPHHSMSQQTRQQQ----QSGQQQQR 3907zebrafish_bassoon_a KPRDARG--SRHYGSSGHS--GRRGHVKHTYHDYDEPP-EDLWPQDDYG-HT---RHISSREHRHHSSGSSGRH--STRHSDEPRSSRSSRS-SKDPSIRHDSRSLSSSGRRGD------SRSQGYH--SSDY---SRDT-SGHHHSSRSGKQSS--------------------SHHQGSSSRKQQD--------HPSS 3913zebrafish_bassoon_b KPREAR---SKHHGSGSHSSSSRRGNVKHTYHDYDEPPEEDLWPQDEYG-HQRHSSSTSSREHRHHGSSS-GRHTSSRHTTDDPRSSRSSRSHPKDPSIRSDGRTTSSSTQKRS-----SDPRSGSHRDSGEY---SRDP-SGHHHGSSGQRGQR----------------------QPGSSRRQEPS-----SGSRPQQ 3532stickleback_bassoon_a KPRSS----SRHYGSSHSSGRSRHG-VKHTYHDYDEPPEEDLWPQDEYG-HGG--RHSTSRDHRHQSSS--GRHSSASRHSDEQRSSRSAKGHPKDPSMRQDPSARSSSTGRRG-----ESRSGGYH--TSDY---SRDP-SGHHHGQRSSRQGD--------------------PHRSSRSKSQQQV----DMQGQPPG 4181stickleback_bassoon_b KPREARSDRSRHHGVH--SSTGRRS--KHTYHDYDEPPEEY-CPQDEYNQQQRHPSSTTPRDHRPHGGSS-GRHSSSRHTSEDPRSTRSSRTHPKDPSVRSDGRGSSSTQRRNG---PDSRSAQGSPRNSGDF---SRDPASGHHHGTAG-RSQR-------------------PQGDRTTPRRQEPS----TAGPKAQQ 3740fugu_bassoon_a KPRSS----SRHYSSSHSSGRSRHG-YKHSYPDYDEPLEEDLWPQDEYG-HGG--RHSSSRDHRHHGSS--GRHSSSSRHSDEPRSSRSSKGHPKDPSVRHDPSGRSSSSGRRG-----DSRSGGYH--SSDY---SRDP-SGHHHGQRSSRQGD--------------------PHRSSRSKSQQGI----DMQGQMTG 4082fugu_bassoon_b KPREARSDRSKHHGSGSHSSTGRRS--KHTHHDYDEPPEEY-CPGEEYS-QQRHTSSSAPREHRHHGSSS-GRHSSSRHTSEDPRSSRSSRTHPKDQTARSDGRGSSSLQRRSG---ADSRSAQASPRNSGDF---SRESDPGRHHGTGG-RGQR--------------------PQGDRTTRRQDPT----ATGSKQQQ 3602medaka_bassoon_b KPREARSDRSKHHGGGSHSSTGRRS--KHTYHDYDEPPEEY-CQQDEYN-QQR--HTSTSREHRHHGSNS-GRHGSSRHGTEDARSSRSSRAHSKDPPGRTDGRSSSSAQRRNV---TDNRSAHGSPRNSGDF---SRDSVSGHHHGTGG-RSQR--------------------PQDRTSTRRQDPS----KPQQIPQG 3681medaka_bassoon_a KPRSS----SRHYSGHS-SGRSRHS-VKHTYHDYDEPPEEDLWPQDEYG-QGG--RHSSSRDHRHHGSS--SRHSSSRH-SDELRSTRSSKGHPKDPLMRHDPSGRSSSSGRRG-----ESRSGGYH--STDY----RDP-SGHHHGQRSSRQGD--------------------PHRSSRSKSQGPV----DMQGQPPG 4051tilapia_bassoon_b KPREARSDRSRHHGSGSHSSTGRRS--KHTYHDYDEPPEEY-GPQDEYN-QRH--SSSTPRDHRHHSSTP-GRHSSSRHTTEDSRSSRSSRTHPKDPSGRSDGRSSSSLQRRTG---ADSRSAQGSPRNSGDF---SRD--SGHHHGTAGRSQRG--------------------QGDRSTSRRQDPT----ATGPKPQQ 3687tilapia_bassoon_a KPRSS----SRHYGSSH-SGRSRHS-VKHTYHDYDEPPEEDLWPQDEYG-HTG--RHSSSRDHRHHGSS--SRHSSSSRHSDEPRSSRSSKGHPKDPSLRHDPSGRSSSSGRRV-----DSRSGGYH--SSDY---SRDS-SGHHHGQRSSRQGD--------------------PHRSSRSKSQGPV----DMQGQPPG 4047cod_bassoon_a -----------------------------------------------------------------------GP--------------------PQGP--QHEARALGAVLLRGE----------------------ARG------------------------------------------------------------- 3655cod_bassoon_b KPREARSDRSRHHGGGGHSSTGRRGNVKHTYHDYDEPPEED-WAQDEYN-QQRHGSSTSSREHRHHGSSSTGRHSSSRHASEDPRASRSSRTHAKDPAARTEGRTSSSAQRRGG---PEARSTQGSHRDSGEF---SRDPTAGHNHGSGG-RGQR-------------------PPGERANSRRPDPSAGHKQTQPQQQQ 3620

: lizard_Bassoon QQPRQQQIGQQQ-----PPSTRQQQQQQQQQQTSRQPQQQQQHQTGPSQPSSQQ-------------QSQQARIQQQQQQPPPQQQQQQ------------------------------------------QPG---TQTQPSSRPAQQQP------GQPQPGGKPQSTLQAQPGTHQTGP---------------PKAE 3965mouse_Bassoon QGRQAAPGPQQS--------QPPSSRQTPSGTASRQPQTQQQQQQQQQQQGL---------------GQQAPQQAPSQARLQPQSQPTT---------------------------------------------------------RGTAP------AASQPAGKPQPGPTTAPGPQPAGP---------------PRAE 3855coelacanth_Bassoon QQQQQQPSSRQE--AAQQQSTRQSQQQQQPPSGQQGPQQQQQQQTRPGQQHVQPSSR----------QQQAAQQQQQQQPPPQQHQPQQ------------------------------------------QAA---QQ--------QQQA------AQQQQQAKQPQTRQPQAGAQQQAM---------------PKVM 4028frog_Bassoon QQQPTQQTSRQQGTGTSQPAAHPSHQAPHQSTTHQTTHQQSQHQQGSQQPTRTQTQQ----------QQQQQQQQQQQQQQPQQLQQQQ------------------------------------------QLG-----------TQGQPS------PKPLQQQQPQQAMKPQQTPQSSTTQSAAQ----------PKAE 4028zebrafish_bassoon_a SRQPGSLGAGQK------GPSGPSGSSRQGVSQPPVDGQQGQRTQLQQQ------------------AQTSAARPGSQTSTAGAVQPQL---------------------------------------------------------VQAQQ------LQAKPGQTGPMPRQPTSAAQTSPAAP-------------IKPE 4013zebrafish_bassoon_b GLGEQQPQSQQG---QQRSSAGQQVSAKQTGSLPPETGQQGQQQLGQTTRTQ---------------QQPLQHPGQQPATTMGASQPTT------------------------------------------TAA-----------GMGTGTTQQ---QQSKPGQAPAQARQQPGGPPTAQPAAAMAKSDIAAPAPAPAPA 3658stickleback_bassoon_a SSRSGSSGRAQG------PAGGPTGSGRQSGPGSQTDGAQGQRTQLQQQAQT---------------SAARSGQPGAAMTTGPQQQPSA------------------------------------------QGQ-----------GMGMGQ------GQAKPGQMGPAGGPMGQARQTGPAGTTPANM--------AKMD 4293stickleback_bassoon_b QQQQPQPPSHGH--AGQQRSGGPGQSGRHPGSEPLDVTQQAQQQHPQSAPPPQQQQ-----------QQQQQQQPLPHSQTPQSSQPTS------------------------------------------TTA-----------GAGPAQ------PQPKSGQA-PLGRQPGVGSVAGQPATT-AKMEAIPPAAAAAAA 3866fugu_bassoon_a SSRSGSSSTRGQ-----GSAGGPAGSGRQSVTGPQSEMGPGQKTQLQQQAQI---------------SAARPGQPGAPAGTGSQQQLST------------------------------------------QGQ-----------GMGMGP------NQAKPGQMGPTGGPMGQTRQTGPQGNTPPTM--------AKID 4195fugu_bassoon_b QPPQSHSGQQRS--------GGQGHSGRHPGSEPLDAPQQAQQ------------------------QHQQNTHPQQRSQTPPHSQPTP------------------------------------------TTT--------TTTTAGPAQ------QQQQQQQQQPKSGPEPSGQPARQPATTNAKMVTTP----SAST 3710medaka_bassoon_b HTGQQRPGGQGQ-------SEGFPGSDHLDGTQQQHQQSANQQLQPQQQPQPSQSSQSTTTPAGLGQAQQQQQQQQQQQQQQQQQQQQQ------------------------------------------QPQ---------------QP------QQPRSAQAPSQGRQPGIGSAPGQPAPMAP----------AASA 3801medaka_bassoon_a SSRSGSSSVRGQ-----GSAGGPAGSGRQGGPGSQTDGTQGQRTPLQQQSQT---------------SANRPGQPGTMTTTGPQQQTPA------------------------------------------QGQ---GMGMGVGLGQGQGQ------GQAKPGQMGPAGGPMGQARQTGPAGTTPSSM--------TKIE 4172tilapia_bassoon_b QQPSQGHSGQQR-------SGGQDQSGRHPGSEPLDGTQHAQQQHHQQNAQQ---------------QQQQQQQQQQQQQQQQQQQPLQ------------------------------------------QGQIQSSQPIPTSAGAGPAQ------QQPKAGQAPAQGRQPGVGSAAGQP-PAAAKMEATP----AAAA 3812tilapia_bassoon_a SSRSGSGSARAQ-----GSAGGPTGSGRQGGPGSQTDGAPGQRAQLQQQAQT---------------SATRPGQPGATTTTASQQQPQA------------------------------------------QGQ---GMGMGMGMGMGMGQ------GQAKPGQMGPAGGPMGQARQTGPTGTTTSTI--------PKID 4168cod_bassoon_a -------------------------------------------------------------------LALRRRIPLGRVLPGPFVR-----------------------------------------------------------------------TPPRPAVLPP-GGPAQVLPQQVPA------------------- 3697cod_bassoon_b QPQPQQQQAQQQ-QQPQQPQQQPGHQQQQPQQPQQQPGHQQQQQQPPQQQQQQQQQQPQHQQQPQHQQQPQHQQQPQHQQPPQQQQQRPGGPAQTGRQSSNETPDGQQQGQQQRHQPQQQAMQQGQTPHPTTGG---PQPTPASAGAGQQQQQQQQLQQPRPGQTPPLGRQPAAGPAAGPPAPTAVKMDHA-----PAAA 3811

* .. :. :. * :*:.:::*:: ******** : lizard_Bassoon HMDASKPTTKVP--QVGKAPQTQPQ-GIAAGGIR--LGPKPAGAPTSA-GAAAGQPGAEGESVFSKILPGGAAEQAGKLTEAVSAFGKKFTSFW 4053mouse_Bassoon QASSSKPPAAKAP-QQGRAPQAQTTPGPGPAGAK--PGARPGGTP----GAPASQPGAEGESVFSKILPGGAAEQAGKLTEAVSAFGKKFSSFW 3942coelacanth_Bassoon PADASKTAGK-PP-QQVKPPQSQPQ-GAAAPGAK--AGQKPVGAP----GSTAGQPGPEGESMFSKILPGGAAEQAGKLTEAVSAFGKKFSSFW 4113frog_Bassoon PLDSSKTAAKPVP-QQARGPQPSMQ-GAATGIK---IGQKPPTAATGATGAAAGQPGTEGESVFSKILPGGAAEQAGKLTEAVSAFGKKFSSFW 4117zebrafish_bassoon_a PTPATAIGAKAVPGQPAKTAQPPLT-GIGSKAAP-----RPGGIG----SAAAGQMGPDGDSMLSKILPGGAAEQAGKLGEAISGFGKKFTSLW 4097zebrafish_bassoon_b PTPAVAIGSKAAP-QAAKPPQIPAT-GIGSKAAP-----RPGGIG----SAA--QQPAEGENVLTKILQGGAAEQAGKLGDAVSALGKKFTSFW 3739stickleback_bassoon_a MPPVTAIGAKAAPVMATKAAQPPLT-GIGSKAAP-----RPGGIG----SAAAGQPGMEGDSMLSKLIPGNAAEQAGKLGDAISGFGKKFTSLF 4377stickleback_bassoon_b TGMKPTTGVPTAT-QPTKIATPPLV-GIGSK-APVGIGSKPVAIG----SAAAGQPPAEGENVLTKILQGGAAEQAGKLGDALSGFGKKFTSLW 3953fugu_bassoon_a TPPATAIGAKAAPVMASKVGQPPLT-GIGSKAAP-----RPGGIG----SAAAGQPGMEGDGMFSKILPGGAAEQAGKLGEAISGFGKKFTSFF 4279fugu_bassoon_b TGIKPSAGIPTVP-PASKITTPPLT-GIGSKAAPVGIGSKAGGIG----STAAGQAPPEGENVLTKILQGGAAEQAGKLGDALSGLGKKFTSFW 3798medaka_bassoon_b AGMKPTAGISSAP-QPSKIPTPPLT-GIGSKAAPVGIGSKPMGIG----TAAAGQVPPEGENVLTKILQGGAAEQAGKLGD------------- 3876medaka_bassoon_a TPPATAIGAKAAPIMASKTAGPPLT-GIGSKAAP-----RPGGIG----SAAAGQPGMEGEGMFSKILPGGAAEQAGKLGEAISGFGKKFTSLF 4256tilapia_bassoon_b TGMKPTTGIPIAP-QPTKIATPPLT-GIGSKAAPVGIGSKPGGIG----SAAAGQAPAEGENVLTKILQGGAAEQAGKLGDALSGLGKKFTSFW 3900tilapia_bassoon_a TPPATAIGAKAAPVLASKTAQPPLT-GIGSKAAP-----RPGGIG----SAAAGQPGMEGEGMFSKILPGGAAEQAGKLGEAISGFGKKFTSFF 4252cod_bassoon_a ---------RGPP-------------GAAPSSKA---GTRPGGIG----SAAAPQPGMDGEGMLSKILPVNAAEQAGKLGD------------- 3749cod_bassoon_b AAMRASPPAAATP---PKTATPPLT-GIGSK-APVGIGSKAGGIG----GATPPQAAADGENVLTKMLPGAAAEQAGKLGDALSGLGKKFTSFW 3896