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List of Chemicals

Sl No. Name of chemical(s) Company Cat No.

1 Thiourea, Hi-AR™/ACS

Himedia

GRM611-500G

2 Potassium sodium tartrate tetrahydrate MB258-500G

3 Dimethyl sulphoxide (DMSO) GRM5856-250ML

4 Sodium carbonate anhydrous MB253-500G

5 Ammonium molybdate tetrahydrate MB249-100G

6 Ammonium chloride, Hi-ARTM/ACS GRM3885-500G

7 Potassium iodide, Hi-AR™/ACS GRM10692-250G

8 Calcium sulphate anhydrous GRM744-500G

9 Potassium persulphate, Hi-AR™/ACS GRM7412-500G

10 Sodium bicarbonate, Hi-AR™ GRM849-500G

11 Sodium sulphate anhydrous, Hi-AR™/ACS GRM3968-500G

12 Zinc nitrate hexahydrate GRM691-500G

13 Zinc acetate dihydrate, Hi-AR™/ACS GRM3977-500G

14 Nickel (II) chloride hexahydrate TC155-500G

15 Ferric chloride hexahydrate TC587E-100G

16 Ferrous sulphate heptahydrate, Hi-AR™/ACS GRM3917-500G

17 Cobalt nitrate hexahydrate PCT0120-100G

18 Cobalt chloride hexahydrate TC107-100G

19 Potassium permanganate PCT1534-500G

20 Manganese (lI) acetate tetrahydrate, Hi-AR™ GRM2298-500G

21 Manganese dioxide, Hi-AR™ GRM7221-250G

22 Copper (II) sulphate pentahydrate PCT0104-250G

23 Copper (II) nitrate trihydrate, Hi-AR™/ACS GRM675-500G

24 EDTA copper (II) disodium salt GRM1680-100G

25 (-)-Riboflavin CMS181-25G

26 Nitroblue Tetrazolium Chloride (NBT) MB107-250MG

27 TEMED, Hi-AR™ GRM1572-250ML

28 Acetate Buffer, pH 5.6 R040-5X100ML

29 Potassium ferricyanide, Hi-AR™/ACS GRM1034-500G

30 NADPH (TPNH) tetrasodium salt RM576-100MG

31 NADP (TPN) disodium salt RM392-100MG

32 Celite GRM226-500G

33 Chitosan PCT0817-500G

34 Synthetic Sea Salt M1344-500G

35 Chitosan (low molecular weight) Sigma 448869-50G

36 Chitosan (medium molecular weight) 448877-50G

37 Chitosan (high molecular weight) 419419-50G

38 Gama aminobutyric acid A2129-10G

39 Ethyl acetate

1.94512.2521

40 Diethyl ether 1.00923.2521

41 Acetone 1.94500.2521

42 Chloroform 1.94506.2521

43 Benzene 1.01782.2521

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44 Toluene Merck Merck

1.08323.2521

45 Methanol 1.94516.2521

46 Sulfuric acid 1.93400.2521

47 Lactic acid 1.93027.0521

48 Glacial acetic acid 1.93402.2521

49 Hydrochloric acid 1.93401.2521

50 Dichloro methane 1.94508.2521

51 Glycerol 1.94501.0521

52 Heptane 1.04365.2521

53 Hexane 1.94514.2521

54 Hydrogen Peroxide 386790-100MLCN

55 Sodium Hexametaphosphate 1.93696.0521

56 1-Butanol 1.94502.2521

57 Formaldehyde 344198-250MLCN

58 Acetone Himedia

AS025-

59 Chloroform 1.94506.2521

60 Methanol 1.94516.2521

61 Sulfuric acid 1.93400.2521

62 Lactic acid 1.93027.0521

63 Glacial acetic acid Merck 1.93402.2521

64 Glycerol Himedia 1.94501.0521

65 Sodium hypochlorite Merck 1.93607.5021

66 Sodium hydroide pellets Himedia 1.93502.0521

67 Mercury(II) chloride Merck 1.04417.0100

68 Formaldehyde solution Himedia 1.04003.2500

69 Fuchsin basic Himedia 6.18024E+13

70 Astra blue FM Merck RM9267-5G

71 phi29 DNA Polymerase (10 U/µL), 1,000

units

ThermoFisher

Scientific

EP0092

72 Pyrophosphatase, inorganic (0.1 U/µL), 10

units

EF0221

73 AccessQuick RT-PCR System,100 reactions

Promega

A1702 74 pGEM-T Easy Vector System I, 20 reactions A1360 75 GoTaq® DNA Polymerase (5 u/μl), 500 U

M3005

76

Diethyl Pyrocarbonate (DEPC) for molecular

biology, 100ml

SRL

6791

0428146

77 TRIzol-S Reagent,

100 ml

50670

BOS002

78 Phenol Tris Equilibrated for molecular

biology Saturated with, 10mM, 500ml

12692

1624262

79 Agar powder -500g Himedia GRM026

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80 DPPH -1g

RM 2798

81 100 ml flask

Borosil

5133016

82 250ml flask 5100021

83 500ml flask 5100024

84 Measuring cylinder -25ml 3026009

85 Measuring cylinder -10ml 3026006

86 Measuring cylinder -100ml 3026016

87 Measuring cylinder -500ml 3026024

88 Amber bottle -250ml 1519021

89 Tubes -15ml 9910007

90 Amber bottle -500ml Borosil 1519024

91 Methanol -2.5lt

Merck

1.945116.2521

92 Ethyl acetate -2.5lt 1.94512.2521

93 Hexane -2.5lt 1.04368.2521

94 Benzene -2.5lt 1.07033.2521

95 Diethylether -2.5lt 1.00923.2521

96 Petroleum benzene -2.5lt 1.94520.2521

97 2-Propanol -2.5lt 1.94524.2521

98 Acetone -2.5lt 1.94500.2521

99 Chloroform -2.5lt 1.94506.2521

100 DNA 100bp Ladder

Promega

G2101

101 GoTaq Start Polymerase M5001

102 Plant DNA kit Qiagen,50 Reaction AS1420

103 Go Taq DNA Polymerase M3005

104 dNTPs mix U1515

105 GoTaq Master Mixes M7122

106 GoTaq G2 Master Mixes M7822

107 PCR Nucleotide Mix C1141

108 GoTaq G2 Flexi DNA Polymerase M7805

109 Whole Genome Sequencing service of one

Rice plant Oryza sativa With the following

Bioinformatics

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30 x genome

coverage with 12 Gb

data. Bioinformatics

analysis, using

Reference

Genome,Nipponbare

110 Service Description

Amplicon Profiling of samples along with bioinformatic analysis:

16S Library Preparation (V3-V4 Region)(for prokaryotic organisms)

ITS library preparation (for fungal strains)

Profiling of amplicons in samples to generate aprrox. 0.2-0.5 million reads/sample

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detailed bioinformatic analysis

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Quotation to be submitted in the following format

Name of the vendor : ………………………………….

Sl No.

Name of the Chemicals Company Quantity Price Discount GST % GST Amount Net Amount Including GST

1 Thiourea, Hi-AR™/ACS

Himedia

2 Potassium sodium tartrate tetrahydrate

3 Dimethyl sulphoxide (DMSO)

4 Sodium carbonate anhydrous

5 Ammonium molybdate tetrahydrate

6 Ammonium chloride, Hi-ARTM/ACS

7 Potassium iodide, Hi-AR™/ACS

8 Calcium sulphate anhydrous

9 Potassium persulphate, Hi-AR™/ACS

10 Sodium bicarbonate, Hi-AR™

11 Sodium sulphate anhydrous, Hi-AR™/ACS

12 Zinc nitrate hexahydrate

13 Zinc acetate dihydrate, Hi-AR™/ACS

14 Nickel (II) chloride hexahydrate

15 Ferric chloride hexahydrate

16 Ferrous sulphate heptahydrate, Hi-AR™/ACS

17 Cobalt nitrate hexahydrate

18 Cobalt chloride hexahydrate

19 Potassium permanganate

20 Manganese (lI) acetate tetrahydrate, Hi-AR™

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21 Manganese dioxide, Hi-AR™ Himedia

22 Copper (II) sulphate pentahydrate

23 Copper (II) nitrate trihydrate, Hi-AR™/ACS

24 EDTA copper (II) disodium salt

25 (-)-Riboflavin

26 Nitroblue Tetrazolium Chloride (NBT)

27 TEMED, Hi-AR™

28 Acetate Buffer, pH 5.6

29 Potassium ferricyanide, Hi-AR™/ACS

30 NADPH (TPNH) tetrasodium salt

31 NADP (TPN) disodium salt

32 Celite

33 Chitosan

34 Synthetic Sea Salt

35 Chitosan (low molecular weight) SIGMA

36 Chitosan (medium molecular weight)

37 Chitosan (high molecular weight)

38 Gama aminobutyric acid

39 Ethyl acetate Merck

40 Diethyl ether

41 Acetone

42 Chloroform

43 Benzene

44 Toluene

45 Methanol

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46 Sulfuric acid Merck

47 Lactic acid

48 Glacial acetic acid

49 Hydrochloric acid

50 Dichloro methane

51 Glycerol

52 Heptane

53 Hexane

54 Hydrogen Peroxide

55 Sodium Hexametaphosphate

56 1-Butanol

57 Formaldehyde

58 Acetone Himedia

59 Chloroform

60 Methanol

61 Sulfuric acid

62 Lactic acid

63 Glacial acetic acid Merck

64 Glycerol Himedia

65 Sodium hypochlorite Merck

66 Sodium hydroide pellets Himedia

67 Mercury(II) chloride Merck

68 Formaldehyde solution Himedia

69 Fuchsin basic Himedia

70 Astra blue FM Merck

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71 phi29 DNA Polymerase (10 U/µL), 1,000 units ThermoFisher

Scientific

72 Pyrophosphatase, inorganic (0.1 U/µL), 10

units

73 AccessQuick RT-PCR System,100 reactions

Promega

74 pGEM-T Easy Vector System I, 20 reactions

75 GoTaq® DNA Polymerase (5 u/μl), 500 U

76 Diethyl Pyrocarbonate (DEPC) for molecular

biology, 100ml SRL

77 TRIzol-S Reagent, 100 ml

78 Phenol Tris Equilibrated for molecular

biology Saturated with, 10mM, 500ml

79 Agar powder -500g Himedia

80 DPPH -1g

81 100 ml flask

Borosil

82 250ml flask

83 500ml flask

84 Measuring cylinder -25ml

85 Measuring cylinder -10ml

86 Measuring cylinder -100ml

87 Measuring cylinder -500ml

88 Amber bottle -250ml

89 Tubes -15ml

90 Amber bottle -500ml

91 Methanol -2.5lt Merck

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92 Ethyl acetate -2.5lt Merck

93 Hexane -2.5lt

94 Benzene -2.5lt

95 Diethylether -2.5lt

96 Petroleum benzene -2.5lt

97 2-Propanol -2.5lt

98 Acetone -2.5lt

99 Chloroform -2.5lt

100 DNA 100bp Ladder Promega

101 GoTaq Start Polymerase

102 Plant DNA kit Qiagen,50 Reaction

103 Go Taq DNA Polymerase

104 dNTPs mix

105 GoTaq Master Mixes

106 GoTaq G2 Master Mixes

107 PCR Nucleotide Mix

108 GoTaq G2 Flexi DNA Polymerase

109 Whole Genome Sequencing service of one

Rice plant Oryza sativa With the following

Bioinformatics

-------------------------

110 Service Description

Amplicon Profiling of samples along with bioinformatic analysis:

16S Library Preparation (V3-V4 Region)(for prokaryotic organisms)

ITS library preparation (for fungal strains)

Profiling of amplicons in samples to

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generate aprrox. 0.2-0.5 million reads/sample

detailed bioinformatic analysis

Signature of the vendor with date & seal of the concern :

Prof. Arnab Sen

Department of Botany, NBU

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