link.springer.com · Web viewProtein profile of cotyledon, tegument, and embryonic axis of mature acorns from a non-orthodox plant species: Quercus ilexCorresponding author; Besma

Protein profile of cotyledon, tegument, and embryonic axis of mature acorns from a non-orthodox plant species: Quercus ilexCorresponding author; Besma Sghaier-Hammami ab, aBiotechnology Centre of Borj Cedria, Laboratory of Extremophile Plants, P. O. Box 901, 2050 Hammam-Lif, Tunisia, b Agroforestry and Plant Biochemistry and Proteomics Research Group, Dpt. Biochemistry and Molecular Biology, University of Cordoba-CeiA3, Cordoba, Spain.

Online resource 5. Differentially abundant identified proteins from 2DE gels. Proteins were extracted from cotyledon, embryonic axis and seed coat of Holm oak (Quercus ilex). C; cotyledon, EA; embryonic axis, T; tegumentProtein namea) Species/Accession

Number b)Spot Numberc)

Mascot scoresd) MPe) Theoretical Experimental tissus

Protein score/Protein score CI%

Total ion score CI%/Total ion score CI%

Mrf) pIg) Mrf) pIg)

MetabolismEnergy metabolismTransketolase, putative Ricinus communis

gi|2555412526801 348/100 261/100 16 81623.4 6.52 100.73 6.44

0

200

400

600

800

1000

1200

1400

1600

1800

2000

cotyledon embryonic axis tegument

Transketolase, putative Ricinus communisgi|255541252

5819 128/100 106/100 5 81623.4 6.52 103.49 6.55

0

50

100

150

200

250

300

350

400


Ribulose 1,5-bisphosphate carboxylase/oxygenase

Puccinellia distans gi|294394

2130 349/100 227/100 17 53444.6 6.04 29.46 5.74

0

200

400

600

800

1000

1200

1400


C EA T

log intensity

RuBisCO large subunit-binding protein subunit beta, chloroplastic

Pisum sativum gi|2506277

1726 795/100 679/100 16 63287.4 5.85 76.16 5.70

0

200

400

600

800

1000

1200


RuBisCO subunit binding-protein beta subunit

Ricinus communis gi|255564820

1728 360/100 277/100 13 64490.9 5.65 72.34 5.63

0

200

400

600

800

1000

1200

1400


ATP synthase beta subunit 1 Gossypium hirsutum gi|242129046

2406 366/100 254/100 16 59987.3 5.9 45.51 5.90

0

100

200

300

400

500

600

700

800


V-type proton ATPase catalytic subunit

Gossypium hirsutum gi|401322

2802 337/100 249/100 15 68764 5.36 85.10 5.78

0

50

100

150

200

250

300

350

400

450


Electron transfer flavoprotein-ubiquinone oxidoreductase, putative


1223 448/100 364/100 13 38039 5.44 38.75 5.65

0

200

400

600

800

1000

1200


Electron transfer flavoprotein-ubiquinone oxidoreductase, putative


112 436/100 342/100 12 38039 5.44 39.46 5.43

0

5000

10000

15000

20000

25000

30000

35000

40000

45000

50000


Carbohydrate metabolismPutative Pyruvate kinase isozyme A, precursor chloroplast

Oryza sativa Japonica Group gi|50508346

1727 145/100 77/100 12 46689.8 5.15 76.86 5.56

0

50

100

150

200

250

300


Pyruvate kinase, putative Ricinus communisgi|255557637

7706 167/100 139/100 7 58042.4 6.26 77.07 6.79

0

100

200

300

400

500

600


Glucose-6-phosphate isomerase,cytosolic

Clarkia arcuata gi|1730166

7724 120/100 64/99.99 10 62848.4 6.51 75.90 7.26

0

200

400

600

800

1000

1200


Glucose-6-phosphate isomerase, putative


1605 421/100 253/100 20 68226.8 5.4 69.25 5.66

0

100

200

300

400

500

600


Phosphoglycerate kinase, chloroplastic

Spinacia oleracea gi|1346698

8405 270/100 226/100 7 45658.3 5.83 50.61 7.14

0

50

100

150

200

250

300

350

400

450

500


Pyrophosphate-fructose 6-phosphate 1-Phosphotransferase subunit alpha


7726 181/100 103/100 14 68173 7.55 88.36 7.24

0

200

400

600

800

1000

1200

1400


Putative pyrophosphate--fructose-6-phosphate 1-phosphotransferase

Arabidopsis thaliana gi|4544445

7626 224/100 163/100 9 51457 6.38 65.08 7.13

0

200

400

600

800

1000

1200

1400


Nodule-enhanced malate dehydrogenase

Pisum sativum gi|3377762

7204 389/100 327/100 10 42106 7.62 41.04 6.73

0

100

200

300

400

500

600

700

800

900

1000


pfkB-type carbohydrate kinase family protein


310 114/100 75/99.999 7 36098.2 4.93 43.10 5.48

0

500

1000

1500

2000

2500

3000


Iron-binding protein Pyrus pyrifoliagi|89276795

1115 193/100 166/100 6 34388.7 6.52 31.65 5.63

0

500

1000

1500

2000

2500


3-phosphoglycerate kinase Populus tremuloides gi|29124969

5417 381/100 335/100 8 42586.7 5.98 50.80 6.63

0

200

400

600

800

1000

1200


cofactor-independent phosphoglyceromutase

Apium graveolens gi|6706331

2718 131/100 123/100 4 61124.8 5.26 82.25 5.66

0

50

100

150

200

250

300

350

400

450

500


Enolase Elaeis guineensisgi|192910834

5614 219/100 133/100 13 48127.5 5.98 67.79 6.70

0

200

400

600

800

1000

1200

1400

1600

1800

2000


UDP-glucose pyrophosphorylase

Dendrocalamus sinicus gi|158705664

7601 320/100 262/100 10 51965.3 5.34 63.73 6.55

0

200

400

600

800

1000

1200

1400

1600

1800


Citrate synthase Citrus sinensis gi|255928569

7622 222/100 192/100 7 52683.4 7.22 60.58 7.09

0

100

200

300

400

500

600


Succinate dehydrogenase, putative


4712 126/100 82/100 9 69147.1 6.18 88.22 6.19

0

50

100

150

200

250


2,3-bisphosphoglycerate-independent phosphoglycerate mutase

Nicotiana tabacum gi|548533

3720 246/100 236/100 4 61257.2 5.98 82.41 6.12

0

200

400

600

800

1000

1200

1400

1600

1800

2000


Pyruvate decarboxylase, putative


3712 257/100 222/100 8 66582.5 5.75 82.93 6.19

0

100

200

300

400

500

600

700


Pyruvate dehydrogenase, putative


2315 271/100 232/100 7 39706.3 5.95 42.47 5.83

0

100

200

300

400

500

600

700

800


haloacid dehalogenase-like hydrolase family protein


6125 124/100 102/100 4 29132.8 6.9 33.06 7.10

0

100

200

300

400

500

600


Delta-1-pyrroline-5-carboxylate dehydrogenase 1 protein

Malus x domestica gi|218963693

6702 121/100 81/100 9 62104.8 6.33 78.54 7.05

0

100

200

300

400

500

600


Ornithine carbamoyltransferase, putative


6408 116/100 93/100 5 41717.1 7.2 43.62 7.10

0

50

100

150

200

250

300

350

400

450


Acetamidase, putative Ricinus communisgi|255568565

4509 167/100 112/100 9 50179.4 6.04 59.89 6.31

0

100

200

300

400

500

600

700

800


Mitochondrial glycine decarboxylase complex P-protein

Populus tremuloides gi|134142800

6805 667/100 584/100 16 116023.1 6.9 118.55 6.94

0

100

200

300

400

500

600

700

800

900


Alcohol dehydrogenase Alnus glutinosagi|71793966

7503 497/100 382/100 14 41655.1 6.28 54.63 6.86

0

500

1000

1500

2000

2500

3000

3500


Amino acid metabolism2-isopropylmalate synthase Micromonas pusilla

CCMP1545 gi|226455646

5726 110/100 72/99.999 10 69051 5.74 83.89 6.50

0

50

100

150

200

250

300


S-adenosylmethionine synthase 2

Elaeagnus umbellatagi|75308025

4504 205/100 135/100 11 43564.9 5.5 54.86 6.09

0

50

100

150

200

250

300

350


3-deoxy-D-arabino-heptulosonate 7-phosphate Synthase

Solanum tuberosum gi|24745903

7625 351/100 272/100 14 60061.5 9.03 66.02 7.07

0

100

200

300

400

500

600

700


Ketol-acid reductoisomerase, chloroplast precursor, putative


4723 237/100 164/100 12 63712.1 6.35 75.41 6.30

0

100

200

300

400

500

600


Putative alanine aminotransferase

Oryza sativa Japonica Group gi|33146868

1503 180/100 152/100 7 53772 5.15 58.62 5.67

0

100

200

300

400

500

600

700

800


Cysteine synthase, putative Ricinus communis gi|255542380

3321 354/100 262/100 11 34435.1 5.5 40.63 6.18

0

1000

2000

3000

4000

5000

6000

7000

8000


Glutamine synthetase Gossypium raimondii gi|159138927

5402 444/100 408/100 6 39417.6 5.64 48.40 6.32

0

500

1000

1500

2000

2500

3000

3500

4000

4500

5000


Lipid metabolismInositol-3-phosphate synthase Hordeum vulgare

gi|145480893703 446/100 398/100 9 56422.6 5.53 71.27 6.02

0

500

1000

1500

2000

2500

3000

3500


Biotin caboxylase subunit (BC) of Het-ACCase


6605 488/100 356/100 19 59472.7 8.01 63.90 6.79

0

20

40

60

80

100

120


UDP-sulfoquinovose synthase Solanum lycopersicum gi|224579299

6626 237/100 213/100 6 53756.3 8.24 60.47 6.84

0

100

200

300

400

500

600

700

800

900


Others metabolismGDP-D-mannose-3',5'-epimerase

Malpighia glabra gi|80973462

6515 487/100 393/100 14 42927.1 5.84 56.11 6.83

0

100

200

300

400

500

600

700

800

900

1000


GDP-D-mannose-3',5'-epimerase

Ribes nigrum gi|223469963

7524 435/100 360/100 13 42940.1 5.88 55.93 6.96

0

50

100

150

200

250

300

350


Nucleoside diphosphate kinase Solanum lycopersicum gi|1346677

6008 101/100 39/98.689 7 15726.3 7.04 18.43 6.97

0

10000

20000

30000

40000

50000

60000


6-phosphogluconate dehydrogenase, putative


7621 243/100 201/100 10 54053.8 6.51 62.10 7.13

0

50

100

150

200

250

300

350

400


Protein synthesis, assembly and degradationGlycyl-tRNA synthetase, putative


4835 395/100 358/100 10 77958.6 6.6 95.44 6.41

0

20

40

60

80

100

120

140

160

180

200


40S ribosomal protein S12, putative

Ricinus communisgi|255572745

5010 194/100 155/100 5 15616.9 5.94 19.35 6.69

0

200

400

600

800

1000

1200

1400

1600


Proteasome subunit beta type 6,9, putative


5101 315/100 267/100 6 24911.5 5.17 31.08 6.24

0

1000

2000

3000

4000

5000

6000


Ras-related protein Rab11C protein transport

Zea mays gi|226491888

7126 225/100 158/100 7 24326.4 6.21 32.10 6.61

0

100

200

300

400

500

600


Putative caffeic acid O-methyltransferase

Betula pendula gi|239586194

3430 239/100 185/100 9 40216.3 5.52 49.14 6.10

0

100

200

300

400

500

600

700

800

900

1000


http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:0015031

Storage proteinsLegumin precursor Quercus robur gi|

14657114209 269/100 256/100 5 55941.2 5.86 29.73 6.18

0

1000

2000

3000

4000

5000

6000

7000

8000

9000

10000


Legumin precursor Quercus robur gi|1465711

7705 224/100 189/100 9 55941.2 5.86 73.09 6.72

0

200

400

600

800

1000

1200

1400

1600



6110 211/100 188/100 7 55941.2 5.86 27.63 6.42

0

500

1000

1500

2000

2500

3000

3500



8119 388/100 343/100 10 55941.2 5.86 31.69 7.20

0

2000

4000

6000

8000

10000

12000



7112 535/100 484/100 10 55941.2 5.86 31.00 6.81

0

5000

10000

15000

20000

25000

30000

35000

40000

45000

50000



6124 389/100 363/100 7 55941.2 5.86 31.96 6.93

0

100

200

300

400

500

600



8705 311/100 266/100 10 55941.2 5.86 74.94 6.96

0

200

400

600

800

1000

1200

1400

1600



8110 138/100 89/100 10 55941.2 5.86 29.02 6.96

0

500

1000

1500

2000

2500

3000

3500


Starch phosphorylase type H Citrus hybrid cultivar gi|21063929

6808 120/100 71/99.999 12 95663.7 6.97 114.93 7.09

0

20

40

60

80

100

120

140

160

180

200


Defense/stressHeat shock 70 kDa protein Zea mays gi|

2294649911835 392/100 294/100 13 70870.9 5.22 91.67 5.63

0

50

100

150

200

250

300

350


Heat shock protein 70 Ageratina adenophora gi|161702921

1707 396/100 357/100 9 71350.2 5.08 85.27 5.59

0

500

1000

1500

2000

2500

3000


Predicted: similar to HT70-1 Vitis vinifera gi|225449497

711 379/100 256/100 19 71525.2 5.17 84.60 5.54

0

500

1000

1500

2000

2500


Predicted: similar to HT70-1 Vitis vinifera gi|225434986

5511 348/100 225/100 18 57349.9 5.17 51.86 6.53

-200

0

200

400

600

800

1000

1200

1400

1600

1800

2000


Heat shock protein, putative Ricinus communis gi|255543357

2804 679/100 588/100 16 71964.7 5.27 93.28 5.80

0

200

400

600

800

1000

1200


Stress and pathogenesis-related protein

Fagus sylvatica gi|3901018

6010 366/100 307/100 7 17418 5.6 20.12 6.96

0

500

1000

1500

2000

2500

3000


Aldo-keto reductase, putative Ricinus communis gi|255543887

8305 129/100 96/100 6 35182.9 5.82 41.44 7.09

0

100

200

300

400

500

600

700


Aldo/keto reductase AKR Manihot eTulenta gi|62526573

5404 314/100 293/100 5 38026.5 6.38 44.69 6.37

0

500

1000

1500

2000

2500


Putative glycine-rich RNA-binding protein

Prunus avium gi|34851124

3106 124/100 78/100 6 17373.9 7.82 20.86 5.90

0

100

200

300

400

500

600

700

800

900


Glycyl-tRNA synthetase, putative


6804 255/100 213/100 11 77958.6 6.6 94.57 6.47

0

50

100

150

200

250

300

350

400

450


Putative glycine-rich RNA-binding protein

Prunus avium gi|34851124

1009 188/100 144/100 6 17373.9 7.82 18.39 5.61

0

500

1000

1500

2000

2500

3000

3500

4000


Thioredoxin fold Arachis hypogaea gi|115187464

3013 474/100 402/100 8 17525.2 5.57 21.58 6.16

0

2000

4000

6000

8000

10000

12000

14000


Manganese superoxide dismutase

ZantedeThia aethiopica gi|3719457

5124 120/100 101/100 4 26316.7 6.71 31.01 6.74

0

10000

20000

30000

40000

50000

60000

70000


Anthranilate N- benzoyltransferase protein, putative


5621 143/100 111/100 7 50429.6 5.38 67.98 6.43

0

500

1000

1500

2000

2500


SignalingOsmotin Gossypium

hirsutum gi|223954264

5123 149/100 136/100 2 13025.2 5.5 28.66 6.49

0

200

400

600

800

1000

1200

1400

1600

1800

2000


Stomatin-1, putative Ricinus communis gi|255557160

6505 155/100 143/100 4 44504 8.62 63.90 6.79

0

200

400

600

800

1000

1200


Others functions

Regulator of ribonuclease activity A, putative


1107 201/100 146/100 7 17958.3 5.39 23.27 5.46

0

1000

2000

3000

4000

5000

6000

7000

8000

9000


Hypothetical protein LOC100191552

Zea mays gi|212276212

7104 196/100 161/100 5 17869.2 6.86 23.22 6.84

0

5000

10000

15000

20000

25000


Aluminum-induced protein Pseudotsuga macrocarpa gi|197309062

7207 101/100 67/99.996 3 12971.5 4.92 36.52 6.69

0

100

200

300

400

500

600

700

800

900

1000


Unnamed protein product Vitis vinifera gi|270241224

4303 101/100 85/100 3 30176.7 5.38 42.39 6.17

0

100

200

300

400

500

600

700


Documents

link.springer.com · Web viewProtein profile of cotyledon, tegument, and embryonic axis of mature acorns from a non-orthodox plant species: Quercus ilexCorresponding author; Besma