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Innova Biosciencesfirst for Antibody labeling
Lightning–Link™
Antibody Labeling Kits
Do you still label antibodies using a multi-step procedure?
Do you lose antibody during the separation steps?
Do you experience batch-to-batch variation?
Then you need:
Lightning–Link
the World’s easiest to use Antibody Labeling Kits
Innova Biosciences Ltdwww.innovabiosciences.com
Innova Biosciences Ltd Babraham Research Campus, Cambridge, UK, CB22 3ATphone: +44 (0)1223 496 170 fax: +44 (0)1223 496 172 [email protected]
Antibody-Label Optimization (Micro-optimizer™)
Optimization of labeled antibodies at the microgram scale. Use our
proprietary technologies to enhance the performance of your labeled
antibodies. By creating an array of trial conjugates (5 formulations per
label) at low microgram scale (20µg per formulation) combined with
selection by you in the assay of interest, the best possible conjugate
can be obtained.
PerfectSCALE™
All conjugates produced using our micro-optimization™ technology
can be scaled over many orders of magnitude without the need for
re-development of the process. The inherent simplicity of Innova’s
technology means that exceptionally high yields are obtained, with
minimal batch-to-batch variation, ensuring consistent performance
with exceptionally high recovery.
Fast & FAb™ Antibody Labeling Service
Antibody labeling service within 48 hours. Using state of the art
labeling chemistry, we have the ability to label microgram to milligram
amounts of your antibody with a variety of popular labels within 48
hours. High quality labeled antibodies delivered in a timely and
e�cient manner.
Oli-Join™
Antibody-oligonucleotide conjugates are the next generation of tools
in biomarker detection, overcoming sensitivity and linear range issues
commonly encountered with standard antibody labels. Through our
Oli-Join™ service, we have the expertise to label an antibody directly
to an oligonucleotide of any given sequence. Furthermore, we have the
ability to create an oligo-antibody hybrid using a small amount of
antibody material – as little as 100ug.
Lightning-Link - Custom Antibody Labeling
Lightning-Link - Frequently Asked Questions
Antibody
Does the antibody have to be purified?
Yes – purified antibodies are perfect for Lightning-Link. Please note
common additives present in bu¡er formulations such as BSA (up to
1%) and gelatin (up to 0.5%) are tolerated - please see our website for
further technical information. Antibodies which are present in ascites
fluid, serum or hybridoma culture media need to be purified.
What is the ideal starting concentration of the antibody?
Antibody concentration should be in the range of 0.5-2.5mg/ml.
Is the Lightning-Link antibody labeling chemistry isotype or species
specific? No.
Methodology
Is the hands-on time really only 30 seconds?
Yes – naturally you will need to familiarize yourself with the
instructions before using Lightning-Link products for the first time,
but after that your hands-on time should be no more than 30 seconds.
How scalable is the technology?
Scalability is one of the major advantages of Lightning-Link. Rather
than risk valuable antibody you can label a small quantity and be
confident that the bulk quantity made later will have essentially
identical properties.
Antibody Labeling Chemistry
What functional groups do I need on my antibody to use
Lightning-Link?
Lightning-Link requires amine groups on the molecule to be labeled.
Most proteins or peptides have lysine and/or alpha-amino groups. All
antibodies have multiple amine functions.
Can proteins other than antibodies be labeled?
Yes – while labeling of primary antibodies is a major application, the
only requirement is that the protein or molecule to be labeled has
amine functionality.
Is the label attached to my protein by a covalent bond?
Yes – conjugates formed with Lightning-Link are perfectly stable.
Will my antibody couple to itself or form high molecular weight
aggregates?
No – the antibody labeling chemistry is unidirectional and therefore
labeling will only occur between the antibody and the label.
Antibody Bu�ers
What bu�er additives can be used and what should be avoided?
Additives such as salts (e.g NaCl), sugars (e.g. sucrose) and
chelators (e.g. EDTA) have no e¡ect on the antibody labeling
reaction. The main additives to avoid are amino acids (e.g. glycine),
blockers (e.g. ethanolamine), tris bu¡er above 20mM and thiols
(DTT, mercaptoethanol).
ITM-2012/05/23
LIFE SCIENCES211 bis, avenue JF Kennedy BP 1140 - 03100 Montluçon Fax 33 (0)4 70 03 82 60
■ Hotline +33 (0)4 70 03 73 06 • [email protected] ■
LIFE SCIENCES211 bis, avenue JF Kennedy BP 1140 - 03100 Montluçon Fax 33 (0)4 70 03 82 60
■ Hotline +33 (0)4 70 03 73 06 • [email protected] ■
Innova Biosciencesfirst for Antibody labeling
Lightning–Link™
Antibody Labeling KitsDo you still label antibodies using a multi-step procedure?
Do you lose antibody during the separation steps?
Do you experience batch-to-batch variation?
Then you need:
Lightning–Linkthe World’s easiest to use Antibody Labeling Kits
Innova Biosciences Ltd www.innovabiosciences.com
, votre distributeur
Innova Biosciencesfirst for Antibody labeling
Lightning–Link™
Antibody Labeling KitsDo you still label antibodies using a multi-step procedure?
Do you lose antibody during the separation steps?
Do you experience batch-to-batch variation?
Then you need:
Lightning–Linkthe World’s easiest to use Antibody Labeling Kits
Innova Biosciences Ltd www.innovabiosciences.com
votre distributeur
30 sec
hands on times !
Les noms et marques cités sur ce document sont la propriété de leurs déposants respectifs.
Lightning–Link™
Lightning-Link kitsThe Lightning-Link kit allows you to label an antibody with minimal hands-on time - less than 30 seconds. Simply add your
antibody to a tube of Lightning-Link mix and the label becomes covalently attached to your antibody in a gentle controlled
process.
As Lightning-Link is a one-step antibody labeling procedure (i.e. is not interrupted by desalting or dialysis steps), your antibody
can be prepared with only microgram quantities of antibody, with recoveries approaching 100%. Furthermore, the technology is
easily scalable (µg to mg) ensuring quality and consistency. Thus Lightning-Link provides a simple antibody labeling method
which saves both time and money.
Over 40 available labels including enzymes, fluorescent protein/dyes, tandems, biotin and avidin
Very easy to use - One Step Format
30 Seconds Hands on Time
100% Antibody Recovery
Freely Scalable
Proven Technology
Lightning-Link process – just add antibody!
For a timed demonstration video of the process please visit our web site.
Labeled Antibody
add the AntibodyLightning-LinkLabel
Perfect reproduction at any scale
Abs
orba
nce
Log conjugate dilution
small scalebulk scale
0.04 5 6 7 8
0.5
1.0
1.5
2.0
2.5
Feature Competitor Competitor Competitor Competitor Competitor Competitor Competitor Lightning A B C D E F G -Link
Hands-on-time 2 mins 20 mins 30 mins 30 mins 30 mins 10 mins 5 mins 30 secs
Column steps No Yes Yes Yes Yes Yes Yes No
# kit components 6 7 5 9 12 6 5 3
# steps 5 11 16 27 31 8 6 3
Antibody labeling time 10 mins 2.5 hours 2 hours 1.5 hours 4 hours 1 hour 1 hour 2 hours
Antibody range 1-20µg 10-20µg 20-100µg 25µg-5mg 100µg 50-200µg 100µg-1mg 10µg-5mg
Conjugation bond non-covalent* covalent covalent covalent covalent covalent covalent covalent
Antibody isotype IgG only IgG only All IgG only IgG only IgG only Undefined All
* The bond between label and antibody is only stable for 30 minutes and therefore must be used within this time limit
Comparison between Lightning-Link and competitors' antibody labeling kits
Innova Biosciencesfirst for Antibody labeling
Phone: +44 (0)1223 496 170
Fluorescencecolor
Maximalemission (nm)
Maximalabsorbance (nm)Fluorescent Dyes
Lightning-Link AMCA 346 442 Blue
Lightning-Link Atto390 390 479 Blue
Lightning-Link Atto425 436 484 Blue-Green
Lightning-Link Atto465 453 508 Green-Yellow
Lightning-Link Atto488 501 523 Green-Yellow
Lightning-Link Fluorescein 485 535 Green-Yellow
Lightning-Link Atto532 532 553 Yellow
Lightning-Link Cy3 550 570 Yellow
Lightning-Link FluoProbes547®H 557 572 Yellow-Orange
Lightning-Link Rhodamine 544 576 Yellow-Orange
Lightning-Link Atto565 563 592 Yellow-Orange
Lightning-Link Cy3.5 581 596 Yellow-Orange
Lightning-Link Texas Red 595 615 Orange-Red
Lightning-Link FluoProbes®594 591 616 Orange-Red
Lightning-Link Atto590 600 627 Orange-Red
Lightning-Link Atto594 601 627 Orange-Red
Lightning-Link Atto633 629 657 Red
Lightning-Link Atto637 630 659 Red
Lightning-Link Cy5 643 667 Red
Lightning-Link FluoProbes®647H 653 675 Red
Lightning-Link Atto655 663 684 Red
Lightning-Link Cy5.5 675 694 Red
Lightning-Link Fluoprobes®682 690 709 Red
Lightning-Link Atto680 670 710 Red
Lightning-Link Atto700 700 725 Red
Lightning-Link Fluoprobes®752 748 772 Near IR
Lightning-Link Cy7 747 776 Near IR
Enzymes
Lightning-Link HRP
Lightning-Link AP
Lightning-Link GOx
Biotin/Streptavidin
Lightning-Link Avidin
Lightning-Link Biotin
Lightning-Link Streptavidin
Fluorescent Proteins
Lightning-Link RPE
Lightning-Link BPE
Lightning-Link APC
Lightning-Link PerCP
Tandems
Lightning-Link PE-Cy5
Lightning-Link PE-Cy5.5
Lightning-Link PE-Cy7
Lightning-Link PerCP-Cy5.5
Lightning-Link APC-Cy5.5
Lightning-Link APC-Cy7
Lightning-Link Range
Interchim - 211 bis, avenue John Fitzgerald Kennedy - BP 1140 - 03100 Montluçon +33 (0)4 70 03 73 06 - Fax +33 (0)4 70 03 82 60 [email protected] - www.interchim.com
Feature Competitor Competitor Competitor Competitor Competitor Competitor Competitor Lightning A B C D E F G -Link
Hands-on-time 2 mins 20 mins 30 mins 30 mins 30 mins 10 mins 5 mins 30 secs
Column steps No Yes Yes Yes Yes Yes Yes No
# kit components 6 7 5 9 12 6 5 3
# steps 5 11 16 27 31 8 6 3
Antibody labeling time 10 mins 2.5 hours 2 hours 1.5 hours 4 hours 1 hour 1 hour 2 hours
Antibody range 1-20µg 10-20µg 20-100µg 25µg-5mg 100µg 50-200µg 100µg-1mg 10µg-5mg
Conjugation bond non-covalent* covalent covalent covalent covalent covalent covalent covalent
Antibody isotype IgG only IgG only All IgG only IgG only IgG only Undefined All
* The bond between label and antibody is only stable for 30 minutes and therefore must be used within this time limit
Comparison between Lightning-Link and competitors' antibody labeling kits
Innova Biosciencesfirst for Antibody labeling
Phone: +44 (0)1223 496 170
Fluorescencecolor
Maximalemission (nm)
Maximalabsorbance (nm)Fluorescent Dyes
Lightning-Link AMCA 346 442 Blue
Lightning-Link Atto390 390 479 Blue
Lightning-Link Atto425 436 484 Blue-Green
Lightning-Link Atto465 453 508 Green-Yellow
Lightning-Link Atto488 501 523 Green-Yellow
Lightning-Link Fluorescein 485 535 Green-Yellow
Lightning-Link Atto532 532 553 Yellow
Lightning-Link Cy3 550 570 Yellow
Lightning-Link FluoProbes547®H 557 572 Yellow-Orange
Lightning-Link Rhodamine 544 576 Yellow-Orange
Lightning-Link Atto565 563 592 Yellow-Orange
Lightning-Link Cy3.5 581 596 Yellow-Orange
Lightning-Link Texas Red 595 615 Orange-Red
Lightning-Link FluoProbes®594 591 616 Orange-Red
Lightning-Link Atto590 600 627 Orange-Red
Lightning-Link Atto594 601 627 Orange-Red
Lightning-Link Atto633 629 657 Red
Lightning-Link Atto637 630 659 Red
Lightning-Link Cy5 643 667 Red
Lightning-Link FluoProbes®647H 653 675 Red
Lightning-Link Atto655 663 684 Red
Lightning-Link Cy5.5 675 694 Red
Lightning-Link Fluoprobes®682 690 709 Red
Lightning-Link Atto680 670 710 Red
Lightning-Link Atto700 700 725 Red
Lightning-Link Fluoprobes®752 748 772 Near IR
Lightning-Link Cy7 747 776 Near IR
Enzymes
Lightning-Link HRP
Lightning-Link AP
Lightning-Link GOx
Biotin/Streptavidin
Lightning-Link Avidin
Lightning-Link Biotin
Lightning-Link Streptavidin
Fluorescent Proteins
Lightning-Link RPE
Lightning-Link BPE
Lightning-Link APC
Lightning-Link PerCP
Tandems
Lightning-Link PE-Cy5
Lightning-Link PE-Cy5.5
Lightning-Link PE-Cy7
Lightning-Link PerCP-Cy5.5
Lightning-Link APC-Cy5.5
Lightning-Link APC-Cy7
Lightning-Link Range
Innova Biosciencesfirst for Antibody labeling
Lightning–Link™
Antibody Labeling KitsDo you still label antibodies using a multi-step procedure?
Do you lose antibody during the separation steps?
Do you experience batch-to-batch variation?
Then you need:
Lightning–Linkthe World’s easiest to use Antibody Labeling Kits
Innova Biosciences Ltd www.innovabiosciences.com
, votre distributeur
Innova Biosciencesfirst for Antibody labeling
Lightning–Link™
Antibody Labeling Kits
Do you still label antibodies using a multi-step procedure?
Do you lose antibody during the separation steps?
Do you experience batch-to-batch variation?
Then you need:
Lightning–Link
the World’s easiest to use Antibody Labeling Kits
Innova Biosciences Ltdwww.innovabiosciences.com
Innova Biosciences Ltd Babraham Research Campus, Cambridge, UK, CB22 3ATphone: +44 (0)1223 496 170 fax: +44 (0)1223 496 172 [email protected]
Antibody-Label Optimization (Micro-optimizer™)
Optimization of labeled antibodies at the microgram scale. Use our
proprietary technologies to enhance the performance of your labeled
antibodies. By creating an array of trial conjugates (5 formulations per
label) at low microgram scale (20µg per formulation) combined with
selection by you in the assay of interest, the best possible conjugate
can be obtained.
PerfectSCALE™
All conjugates produced using our micro-optimization™ technology
can be scaled over many orders of magnitude without the need for
re-development of the process. The inherent simplicity of Innova’s
technology means that exceptionally high yields are obtained, with
minimal batch-to-batch variation, ensuring consistent performance
with exceptionally high recovery.
Fast & FAb™ Antibody Labeling Service
Antibody labeling service within 48 hours. Using state of the art
labeling chemistry, we have the ability to label microgram to milligram
amounts of your antibody with a variety of popular labels within 48
hours. High quality labeled antibodies delivered in a timely and
e�cient manner.
Oli-Join™
Antibody-oligonucleotide conjugates are the next generation of tools
in biomarker detection, overcoming sensitivity and linear range issues
commonly encountered with standard antibody labels. Through our
Oli-Join™ service, we have the expertise to label an antibody directly
to an oligonucleotide of any given sequence. Furthermore, we have the
ability to create an oligo-antibody hybrid using a small amount of
antibody material – as little as 100ug.
Lightning-Link - Custom Antibody Labeling
Lightning-Link - Frequently Asked Questions
Antibody
Does the antibody have to be purified?
Yes – purified antibodies are perfect for Lightning-Link. Please note
common additives present in bu¡er formulations such as BSA (up to
1%) and gelatin (up to 0.5%) are tolerated - please see our website for
further technical information. Antibodies which are present in ascites
fluid, serum or hybridoma culture media need to be purified.
What is the ideal starting concentration of the antibody?
Antibody concentration should be in the range of 0.5-2.5mg/ml.
Is the Lightning-Link antibody labeling chemistry isotype or species
specific? No.
Methodology
Is the hands-on time really only 30 seconds?
Yes – naturally you will need to familiarize yourself with the
instructions before using Lightning-Link products for the first time,
but after that your hands-on time should be no more than 30 seconds.
How scalable is the technology?
Scalability is one of the major advantages of Lightning-Link. Rather
than risk valuable antibody you can label a small quantity and be
confident that the bulk quantity made later will have essentially
identical properties.
Antibody Labeling Chemistry
What functional groups do I need on my antibody to use
Lightning-Link?
Lightning-Link requires amine groups on the molecule to be labeled.
Most proteins or peptides have lysine and/or alpha-amino groups. All
antibodies have multiple amine functions.
Can proteins other than antibodies be labeled?
Yes – while labeling of primary antibodies is a major application, the
only requirement is that the protein or molecule to be labeled has
amine functionality.
Is the label attached to my protein by a covalent bond?
Yes – conjugates formed with Lightning-Link are perfectly stable.
Will my antibody couple to itself or form high molecular weight
aggregates?
No – the antibody labeling chemistry is unidirectional and therefore
labeling will only occur between the antibody and the label.
Antibody Bu�ers
What bu�er additives can be used and what should be avoided?
Additives such as salts (e.g NaCl), sugars (e.g. sucrose) and
chelators (e.g. EDTA) have no e¡ect on the antibody labeling
reaction. The main additives to avoid are amino acids (e.g. glycine),
blockers (e.g. ethanolamine), tris bu¡er above 20mM and thiols
(DTT, mercaptoethanol).
ITM-2012/05/23
LIFE SCIENCES211 bis, avenue JF Kennedy BP 1140 - 03100 Montluçon Fax 33 (0)4 70 03 82 60
■ Hotline +33 (0)4 70 03 73 06 • [email protected] ■
LIFE SCIENCES211 bis, avenue JF Kennedy BP 1140 - 03100 Montluçon Fax 33 (0)4 70 03 82 60
■ Hotline +33 (0)4 70 03 73 06 • [email protected] ■
Innova Biosciencesfirst for Antibody labeling
Lightning–Link™
Antibody Labeling KitsDo you still label antibodies using a multi-step procedure?
Do you lose antibody during the separation steps?
Do you experience batch-to-batch variation?
Then you need:
Lightning–Linkthe World’s easiest to use Antibody Labeling Kits
Innova Biosciences Ltd www.innovabiosciences.com
, votre distributeur
Innova Biosciencesfirst for Antibody labeling
Lightning–Link™
Antibody Labeling KitsDo you still label antibodies using a multi-step procedure?
Do you lose antibody during the separation steps?
Do you experience batch-to-batch variation?
Then you need:
Lightning–Linkthe World’s easiest to use Antibody Labeling Kits
Innova Biosciences Ltd www.innovabiosciences.com
votre distributeur
30 sec
hands on times !
Les noms et marques cités sur ce document sont la propriété de leurs déposants respectifs.
