LIFE SCIENCES - Springer · 2012. 4. 27. · (mulberry, pea, Indian mustard) under environmental stress. Prof. M.N.V. Prasad is a Professor in the Department of Plant Sciences at

springer.com 2

A.M. Abbasi, Quaid-i-Azam University, Islamabad, Pakistan;M.A. Khan, Quaid-i-Azam University, Islamabad, Pakistan; M.Ahmad, Quaid-i-Azam University, Islamabad, Pakistan; M.Zafar, Quaid-i-Azam University, Islamabad, Pakistan

Medicinal Plant Biodiversity ofLesser Himalayas-PakistanThe Himalayan region is among the largestmountains systems of the world with uncountedunique medicinal plants resources. The lesserHimalayas ranges are the extension of GreaterHimalayas. They have unique ecology, vegetationand diversity of medicinal flora due to tremendousvariation in the altitude, climate and associatedwildlife. The utilization of medicinal plants inmedicine suffers from the fact that although plants areused to treat diseases, scientific evidence is lacking inmany cases. Different societies of the world use theplants according to their own beliefs and knowledgeand previous experiences. Their knowledge aboutthe use of the plants is usually not known to theother world or science. This book provides a briefintroduction of Lesser Himalayas, ethnobotanicalaspects, marketing and anthropogenic pressure onmedicinal flora. It comprises one hundred medicinalplant species including Pteridophytes, Gymnospermsand Angiosperms (Monocots and Dicots) alongwith their scientific description and traditional uses. Arshad Mehmood Abbasi has obtained his M.Phil. in 2007 in Taxonomic and Ethnobotany ofMedicinal Plants from Quaid-i-Azam University,Islamabad, Pakistan. He has published 13 researchpapers in national and international journals ofrepute. His research work concentrates on medicinalplants, ethnobotany, biodiversity, wild edible foodresources (fruits and vegetables). Currently he is PhDscholar and Research Associate in the Departmentof Plant Sciences, Quaid-i-azam University,Islamabad, Pakistan. Prof. Dr. Mir Ajab Khan hasobtained Ph.D. in 1984 in Plant bio-systematicsfrom Leicester University England. His researchwork has concentrated on Asteraceae, Poaceae,Rosaceae, Labiatae, Scrophulariaceae, Boraginaceae,Euphorbiaceae and Solanaceae of flowering plants.He has taught 32 Ph.D. and 75 M.Phil students inpure and applied disciplines of plant sciences. He haspublished seven books and 311 scientific papers indifferent national and foreign journals. Dr. MushtaqAhmad obtained his Ph.D. in 2008 in plant taxonomyfrom Quaid-i-Azam University, Islamabad, Pakistan.He has published 135 research papers in nationaland international journals of repute. He is authorof six books. His research work has concentratedon authentication of problematic medicinal plants,fingerprinting, SEM techniques, biodiversity andbiofuel technology. He is an Assistant Professor inthe Department of Plant Sciences, Quaid-i-Azam

University, Islamabad, Pakistan. Dr. MuhammadZafar is herbarium botanist in the Departmentof Plant Sciences at Quaid-i-Azam University,Islamabad, Pakistan. He is author of three books.He has published 88 research papers in differentnational and international journals of repute. Hisresearch interests are medicinal plants and herbariummanagement.

More on www.springer.com/978-1-4614-1574-9Usually dispatched within 3 to 5 business days.

2012. XIII, 220 p. 116 illus. in color. Hardcover978-1-4614-1574-9

▶139,95 €

P. Ahmad, Amar Singh College, Srinagar, J&K, India; M.N.V.Prasad, University of Hyderabad, India (Eds.)

Abiotic Stress Responses in PlantsMetabolism, Productivity and Sustainability

Abiotic stress cause changes in soil-plant-atmospherecontinuum and is responsible for reduced yield inseveral major crops. Therefore, the subject of abioticstress response in plants - metabolism, productivityand sustainability - is gaining considerablesignificance in the contemporary world. Abiotic stressis an integral part of “climate change,” a complexphenomenon with a wide range of unpredictableimpacts on the environment. Prolonged exposureto these abiotic stresses results in altered metabolismand damage to biomolecules. Plants evolve defensemechanisms to tolerate these stresses by upregulationof osmolytes, osmoprotectants, and enzymatic andnon-enzymatic antioxidants, etc. This volume dealswith abiotic stress-induced morphological andanatomical changes, abberations in metabolism,strategies and approaches to increase salt tolerance,managing the drought stress, sustainable fruitproduction and postharvest stress treatments, roleof glutathione reductase, flavonoids as antioxidantsin plants, the role of salicylic acid and trehalose inplants, stress-induced flowering. The role of soilorganic matter in mineral nutrition and fatty acidprofile in response to heavy metal stress are also dealtwith. Proteomic markers for oxidative stress as a newtools for reactive oxygen species and photosynthesisresearch, abscisic acid signaling in plants are coveredwith chosen examples. Stress responsive genes andgene products including expressed proteins thatare implicated in conferring tolerance to the plantare presented. Thus, this volume would providesthe reader with a wide spectrum of informationincluding key references and with a large numberof illustrations and tables. Dr. Parvaiz is AssistantProfessor in Botany at A.S. College, Srinagar, Jammuand Kashmir, India. He has completed his post-graduation in Botany in 2000 from Jamia Hamdard

New Delhi India. After his Ph.D from the IndianInstitute of Technology (IIT) Delhi, India in 2007he joined the International Centre for GeneticEngineering and Biotechnology, New Delhi. Hehas published more than 20 research papers in peerreviewed journals and 4 book chapters. He has alsoedited a volume which is in press with StudiumPress Pvt. India Ltd., New Delhi, India. Dr. Parvaizis actively engaged in studying the molecular andphysio-biochemical responses of different plants(mulberry, pea, Indian mustard) under environmentalstress. Prof. M.N.V. Prasad is a Professor in theDepartment of Plant Sciences at the University ofHyderabad, India. He received B.Sc. (1973) andM.Sc. (1975) degrees from Andhra University, India,and the Ph.D. degree (1979) in botany from theUniversity of Lucknow, India. Prasad has published216 articles in peer reviewed journals and 82 bookchapters and conference proceedings in the broadarea of environmental botany and heavy metal stressin plants. He is the author, co-author, editor, or co-editor for eight books. He is the recipient of PitamberPant National Environment Fellowship of 2007awarded by the Ministry of Environment and Forests,Government of India.


2012. XV, 473 p. 61 illus., 14 in color. Hardcover978-1-4614-0633-4

▶169,95 €

P. Ahmad, University of Kashmir, Srinagar, India; M.N.V.Prasad, University of Hyderabad, India (Eds.)

Environmental Adaptations andStress Tolerance of Plants in theEra of Climate ChangeClimate change is a complex phenomenon witha wide range of impacts on the environment.Biotic and abiotic stress are a result of climatechange. Abiotic stress is caused by primary andsecondary stresses which are an impediment toplant productivity. Prolonged exposure to thesestresses results in altered metabolism and damage tobiomolecules. Plants evolve defense mechanisms towithstand these stresses, e.g. synthesis of osmolytes,osmoprotectants, and antioxidants. Stress responsivegenes and gene products including expressedproteins are implicated in conferring tolerance tothe plant. This volume will provide the reader witha wide spectrum of information, including vitalreferences. It also provides information as to howphytoconstituents, hormones and plant associatedmicrobes help the plants to tolerate the stress. Thisvolume also highlights the use of plant resourcesfor ameliorating soil contaminants such as heavy

http://www.springer.com/978-1-4614-1574-9http://www.springer.com/978-1-4614-0633-4

springer.com 3

metals. Dr. Parvaiz is Assistant professor in Botanyat A.S. College, Srinagar, Jammu and Kashmir, India.He has completed his post-graduation in Botany in2000 from Jamia Hamdard New Delhi India. Afterhis Ph.D from the Indian Institute of Technology(IIT) Delhi, India in 2007 he joined the InternationalCentre for Genetic Engineering and Biotechnology,New Delhi. He has published more than 20 researchpapers in peer reviewed journals and 4 book chapters.He has also edited a volume which is in press withStudium Press Pvt. India Ltd., New Delhi, India. Dr.Parvaiz is actively engaged in studying the molecularand physio-biochemical responses of different plants(mulberry, pea, Indian mustard) under environmentalstress. Prof. M.N.V. Prasad is a Professor in theDepartment of Plant Sciences at the University ofHyderabad, India. He received B.Sc. (1973) andM.Sc. (1975) degrees from Andhra University, India,and the Ph.D. degree (1979) in botany from theUniversity of Lucknow, India. Prasad had published216 articles in peer reviewed journals and 82 bookchapters and conference proceedings in the broadarea of environmental botany and heavy metal stressin plants. He is the author, co-author, editor, or co-editor for eight books. He is the recipient of PitamberPant national Environment Fellowship of 2007awarded by the Ministry of Environment and Forests,Government of India.


2012. XVI, 515 p. 85 illus., 40 in color. Hardcover978-1-4614-0814-7

▶169,95 €

D.S. Allan, University of Ottawa, ON, Canada; D. Strunk,Medical University of Graz, Austria (Eds.)

Regenerative Therapy UsingBlood-Derived Stem CellsRegenerative medicine is the emerging scienceof how we can support our body to repair itselfand encompasses innovative strategies to facilitaterecovery after organ injury. Several types of blood-derived stem cells can be easily procured and havethe potential to facilitate this repair process. Ourunderstanding of the various cell types and precisemechanisms of repair are evolving quickly. In thisbook, several key types of blood-derived stem cellsthat participate in the repair of organ damage arereviewed and specific examples of how stem cells mayimprove heart damage and neurological injury areaddressed. Practical approaches to preparing cellularproducts and regulatory issues that will governtranslational studies in patients are also covered.Regenerative Therapy Using Blood-Derived Stem

Cells is a timely publication of a fast-moving field thatwill stimulate further research and enquiry into thepromising field of regenerative medicine.


2012. XII, 217 p. 18 illus., 12 in color. (Stem Cell Biology andRegenerative Medicine) Hardcover978-1-61779-470-4

▶139,95 €

M.A. Alterman, FDA, Bethesda, MD, USA; P. Hunziker,University of Zurich, Switzerland (Eds.)

Amino Acid AnalysisMethods and Protocols

Amino Acid Analysis (AAA) is an integral partof analytical biochemistry. In a relatively shorttime, the variety of AAA methods has evolveddramatically with more methods shifting to the useof mass spectrometry (MS) as a detection method.Another new aspect is miniaturization. However,most importantly, AAA in this day and age shouldbe viewed in the context of Metabolomics as a part ofSystems Biology. Amino Acid Analysis: Methods andProtocols presents a broad spectrum of all availablemethods allowing for readers to choose the methodthat most suits their particular laboratory set-upand analytical needs. In this volume, a reader canfind chapters describing general as well as specificapproaches to the sample preparation. A numberof chapters describe specific applications of AAAin clinical chemistry as well as in food analysis,microbiology, marine biology, drug metabolism,even archeology. Separate chapters are devoted tothe application of AAA for protein quantitation andchiral AAA. Written in the highly successful Methodsin Molecular Biology™ series format, chapterscontain introductions to their respective topics,lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols,and notes on troubleshooting and avoiding knownpitfalls. Authoritative and accessible, Amino AcidAnalysis: Methods and Protocols provides crucialtechniques that can be applied across multipledisciplines by anyone involved in biomedical researchor life sciences.


2012. XIV, 363 p. 78 illus., 7 in color. (Methods in MolecularBiology, Vol. 828) Hardcover978-1-61779-444-5

▶109,95 €

M. Amit, Technion – Israel Institute of Technology, Haifa,Israel; J. Itskovitz-Eldor, Technion – Israel Institute ofTechnology, Haifa, Israel (Eds.)

Atlas of Human Pluripotent StemCellsDerivation and Culturing

Human pluripotent stem cells, including humanembryonic stem cells and induced pluripotentstem cells, are a key focus of current biomedicalresearch. The emergence of state of the art culturingtechniques is promoting the realization of the fullpotential of pluripotent stem cells in basic andtranslational research and in cell-based therapies. Thiscomprehensive and authoritative atlas summarizesmore than a decade of experience accumulated by aleading research team in this field. Hands-on step-by-step guidance for the derivation and culturing ofhuman pluripotent stem cells in defined conditions(animal product-free, serum-free, feeder-free) andin non-adhesion suspension culture are provided,as well as methods for examining pluripotency(embryoid body and teratoma formation) andkaryotype stability. The Atlas of Human PluripotentStem Cells - Derivation and Culturing will serveas a reference and guide to established researchersand those wishing to enter the promising field ofpluripotent stem cell research.

More on www.springer.com/978-1-61779-547-3Usually dispatched within 3 to 5 business days. November24, 2011

2012. XIV, 142 p. 105 illus., 88 in color. (Stem Cell Biologyand Regenerative Medicine) Hardcover978-1-61779-547-3

▶99,95 €

R.B. Ashman, University of Queensland, Brisbane, QLD,Australia (Ed.)

LeucocytesMethods and Protocols

The innate immune response is a crucial componentof early resistance to infection, and it is now revealingincreasing levels of complexity. The ability to modify

http://www.springer.com/978-1-4614-0814-7http://www.springer.com/978-1-61779-470-4http://www.springer.com/978-1-61779-444-5http://www.springer.com/978-1-61779-547-3

4 springer.com

the genome in vivo, has facilitated understanding ofcomplex interactions between leucocytes and othercomponents of the immune system, and phenotype-driven strategies using chemical mutagenesishave placed another powerful weapon in thearmamentarium. In, Leucocytes: Methods andProtocols provides detailed protocols and practicaladvice on a variety of modern approaches to thestudy of leucocytes and their products. Written inthe highly successful Methods in Molecular Biology™series format, chapters include introductions to theirrespective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratoryprotocols, and key tips on troubleshooting andavoiding known pitfalls.Authoritative and practical,Leucocytes:Methods and Protocols seeks to aidscientists in further study into the immune systemand leucocytes.


2012. XI, 296 p. 62 illus., 24 in color. (Methods in MolecularBiology, Vol. 844) Hardcover978-1-61779-526-8

▶94,95 €

M.L. Badenes, Instituto Valenciano de InvestigacionesAgrarias (IVIA), Valencia, Spain; D.H. Byrne, Texas A&MUniversity, College Station, TX, USA (Eds.)

Fruit BreedingFruit Breeding is the eighth volume in the Handbookof Plant Breeding series. Like the other volumesin the series, this volume presents information onthe latest scientific information in applied plantbreeding using the current advances in the field,from an efficient use of genetic resources to theimpact of biotechnology in plant breeding. Themajority of the volume showcases individual crops,complemented by sections dealing with importantaspects of fruit breeding as trends, marketing andprotection of new varieties, health benefits of fruitsand new crops in the horizon. The book also featurescontributions from outstanding scientists for eachcrop species.Maria Luisa BadenesInstituto Valencianode Investigaciones Agrarias (IVIA), Valencia,SpainDavid ByrneDepartment of HorticulturalSciences, Texas A&M University, College Station, TX,USA


2012. XVI, 875 p. 83 illus., 41 in color. (Handbook of PlantBreeding, Vol. 8) Hardcover978-1-4419-0762-2

▶199,95 €

H.W. Bass, Florida State University, Tallahassee, FL, USA; J.A.Birchler, University of Missouri, Columbia, MO, USA (Eds.)

Plant CytogeneticsGenome Structure and Chromosome Function

This reference book provides information onplant cytogenetics for students, instructors, andresearchers. Topics covered by international expertsinclude classical cytogenetics of plant genomes;plant chromosome structure; functional, molecularcytology; and genome dynamics. In addition, chaptersare included on several methods in plant cytogenetics,informatics, and even laboratory exercises for aspiringor practiced instructors. The book provides a uniquecombination of historical and modern subject matter,revealing the central role of plant cytogenetics in plantgenetics and genomics as currently practiced. Thisbreadth of coverage, together with the inclusion ofmethods and instruction, is intended to convey a deepand useful appreciation for plant cytogenetics. Wehope it will inform and inspire students, researchers,and teachers to continue to employ plant cytogeneticsto address fundamental questions about the cytologyof plant chromosomes and genomes for years tocome.Hank W. Bass is a Professor in the Departmentof Biological Science at Florida State University.JamesA. Birchler is a Professor in the Division of BiologicalSciences at the University of Missouri.


2012. X, 345 p. 70 illus., 53 in color. (Plant Genetics andGenomics: Crops and Models, Vol. 4) Hardcover978-0-387-70868-3

▶149,95 €

E. Biancardi, Consiglio per la Ricerca e la Sperimentazionein Agricoltura, Rovigo, Italy; L.W. Panella, USDA-ARS, FortCollins, CO, USA; R.T. Lewellen, USDA-ARS, Salinas, CA, USA

Beta maritimaThe Origin of Beets

Along the undisturbed shores, especially of theMediterranean Sea and the European North AtlanticOcean, is a quite widespread plant called Beta

maritima by botanists, or more commonly seabeet. Nothing, for the inexperienced observer's eye,distinguishes it from surrounding wild vegetation.Despite its inconspicuous and nearly invisible flowers,the plant has had and will have invaluable economicand scientific importance. Indeed, according to Linnè,it is considered "the progenitor of the beet cropspossibly born from Beta maritima in some foreigncountry". Recent molecular research confirmedthis lineage. Selection applied after domesticationhas created many cultivated types with differentdestinations. The wild plant always has beenharvested and used both for food and as a medicinalherb. Sea beet crosses easily with the cultivatedtypes. This facilitates the transmission of genetictraits lost during domestication, which selectionprocesses aimed only at features immediately usefulto farmers and consumers may have depleted. Indeed,as with several crop wild relatives, Beta maritima hasbeen successfully used to improve cultivated beet’sgenetic resistances against many diseases and pests.In fact, sugar beet cultivation currently would beimpossible in many countries without the recoveryof traits preserved in the wild germplasm. Dr. EnricoBiancardi graduated from Bologna University.From 1977 until 2009, he was involved in sugarbeet breeding activity by the Istituto Sperimentaleper le Colture Industriali (ISCI) formerly StazioneSperimentale di Bieticoltura (Rovigo, Italy), wherehe released rhizomania and cercospora resistantgermplasm and collected seeds of Mediterranean seabeet populations as a genetic resource for breedingand ex situ conservation. Retired since 2009, hestill collaborates with several working breeders,in particular, at the USDA Agricultural ResearchStations, at the Chinese Academy of AgriculturalScience (CAAS), and at the Athens University (AUA).He has edited books, books chapters and authoredmore than 150 papers.Dr. Lee Panella is a plantbreeder and geneticist with the USDA-ARS at FortCollins, Colorado. He earned his B.S. in Crop andSoil Science from Michigan State University, an M.S.in Plant Breeding from Texas A&M University, anda Ph.D. in genetics from the University of Californiaat Davis. His research focus is developing diseaseresistant germplasm using sugar beet wild relatives. He is chairman of the USDA-ARS Sugar Beet CropGermplasm Committee and has collected and workedextensively with sea beet. Dr. Robert T. Lewellen wasraised on a ranch in Eastern Oregon and obtained aB.S. in Crop Science from Oregon State Universityfollowed by a Ph.D. from Montana State Universityin Genetics. From 1966 to 2008 he was a researchgeneticist for the USDA-ARS at Salinas, California,where he studied the genetics of sugar beet and as aplant breeder, often used sea beet as a genetic sourceto produce many pest and disease resistant sugar beetgermplasm and parental lines, while authoring morethan 100 publications.


2012. XVII, 293 p. 126 illus., 81 in color. Hardcover978-1-4614-0841-3

▶149,95 €


springer.com 5

M.D. Bolton, USDA Agricultural Research Service, Fargo,ND, USA; B.P.H.J. Thomma, Wageningen University, TheNetherlands (Eds.)

Plant Fungal PathogensMethods and Protocols

Over the course of evolution, fungi have adapted tooccupy specific niches, from symbiotically inhabitingthe flora of the intestinal tract of mammals tosaprophytic growth on leaf litter resting on theforest floor. In Plant Fungal Pathogens: Methodsand Protocols, expert researchers in the field detailmany of the methods which are now commonlyused to study fungal plant pathogens. These includemethods and techniques for model systems such asArabidopsis thaliana as well as crop plants, aspects offungal biology, genome annotation, next-generationsequencing, and fungal transformation and moleculartools for disease and/or pathogen quantification thatare critical for revealing the role for a fungal geneof interest in disease development. Written in thehighly successful Methods in Molecular Biology™series format, chapters include introductions to theirrespective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratoryprotocols, and key tips on troubleshooting andavoiding known pitfalls. Authoritative and practical,Plant Fungal Pathogens: Methods and Protocolsseeks to aid scientists in the further study in currenttechniques that cover a wide-range of methods tostudy molecular aspects of pathogenesis.


2012. XVI, 648 p. 138 illus., 72 in color. (Methods inMolecular Biology, Vol. 835) Hardcover978-1-61779-500-8

▶124,95 €

A.C. Brand, University of Aberdeen, UK; D.M. MacCallum,University of Aberdeen, UK (Eds.)

Host-Fungus InteractionsMethods and Protocols

Microbiologists, medical mycologists, immunologists,and biochemists are increasingly working together

to focus on the processes involved in the progressionand treatment of fungal disease. Host-FungusInteractions: Methods and Protocols is designedfor research scientists who are involved in thiswork and interested in undertaking new orcomparative studies of interactions between themammalian host and clinically important fungalpathogens. Aiming to combine approaches forreverse genetics in pathogenic fungi with methodsfor their application in in vitro and in vivo modelsof disease, the book includes methods for theculture and genetic manipulation of the primaryfungal pathogens and the opportunistic pathogens,as well as methods for investigating host-fungusinteractions in model systems. Written in the highlysuccessful Methods in Molecular Biology™ seriesformat, chapters include introductions to theirrespective topics, lists of the necessary materialsand reagents, step-by-step, readily reproduciblelaboratory protocols, and tips on troubleshootingand avoiding known pitfalls. Comprehensive andpractical, Host-Fungus Interactions: Methods andProtocols describes available molecular methodsand fungal infection models in great detail in orderto encourage researchers to try new approaches toinvestigating host-fungus interactions with addedlevels of confidence.



▶124,95 €

W.-Y. Chan, The Chinese University of Hong Kong, Shatin,N.T., Hong Kong; L.A. Blomberg, ARS-USDA, Beltsville, MD,USA (Eds.)

Germline DevelopmentMethods and Protocols

The germline is unique in mammals as it is theonly cell lineage that undergoes mitosis, meiosis,and differentiation, making these cells amenableto in-depth genomic and mechanistic studies. InGermline Development: Methods and Protocols,expert researchers provide a wide-ranging collectionof techniques that can be applied to the study of boththe male and female germline. As the techniquesused for isolating and studying male and female germcells are slightly dissimilar, the different technologiesare grouped under two sections in this volume,namely, male germline and female germline. Thiscollection brings the field of germline developmentalstudies together such that readers can find, in a singlevolume, techniques that allow the isolation of gonadswith germ cells at different stages of development,the study of the genomics and epigenomics of germ

cells, and an examination of the role of single genesin the developmental potential and function of germcells. Written in the highly successful Methods inMolecular Biology™ series format, chapters includeintroductions to their respective topics, lists of thenecessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tipson troubleshooting and the avoidance of knownpitfalls. Comprehensive and cutting-edge, GermlineDevelopment: Methods and Protocols serves asa comprehensive text to individuals, particularlyresearchers in reproductive and developmentalbiology, who are interested in studying cells of thegerm lineage.



▶109,95 €

Q. Cheng, Dupont Central Research and Development,Wilmington, DE, USA (Ed.)

Microbial Metabolic EngineeringMethods and Protocols

Metabolic engineering is the practice of geneticallyoptimizing metabolic and regulatory networkswithin cells to increase production and/or recoveryof certain substance from cells. In MicrobialMetabolic Engineering: Methods and Protocolsexpert researchers in the field detail many of themethods which are now commonly used to studymetabolic engineering. These include methodsand techniques to engineer genes and pathways,use of modern biotechnology tools in microbialmetabolic engineering, and examples of metabolicengineering for real world applications such aswhole cell biosensors and acetate control in largescale fermentation. Written in the highly successfulMethods in Molecular Biology™ series format,chapters include introductions to their respectivetopics, lists of the necessary materials and reagents,step-by-step, readily reproducible laboratoryprotocols, and key tips on troubleshooting andavoiding known pitfalls. Authoritative and practical,Microbial Metabolic Engineering: Methods andProtocols seeks to provide researchers with anoverview of key topics on microbial metabolicengineering.


2012. XII, 319 p. 62 illus., 4 in color. (Methods in MolecularBiology, Vol. 834) Hardcover978-1-61779-482-7

▶109,95 €


6 springer.com

Y. Chevolot, Institut des Nanotechnologies de Lyon (INL),Ecully, France (Ed.)

Carbohydrate MicroarraysMethods and Protocols

Carbohydrate microarrays emerged as a keytechnology for the deciphering of the glycospaceby providing a multiplex technology where tens tohundreds of carbohydrates/protein interactions canbe probed in parallel. Carbohydrate Microarrays:Methods and Protocols aims to give the reader thetheoretical and experimental clues necessary for thefabrication and implementation of carbohydratemicroarrays. This requires three essential steps: 1)to obtain the carbohydrate probes (monosacharides,oligosacchrides, polysacchairdes, glycoconjugatesor glycoclusters), 2) to immobilize these probes,and 3) to implement the protocols for biological/biochemical interaction with the desired target. Thisvolume gives an overview of carbohydrate microarrayand carbohydrate chemistry and illustrates differentdetection techniques and their applications. Writtenin the successful Methods in Molecular Biology™series format, chapters include introductions to theirrespective topics, lists of the necessary materialsand reagents, step-by-step, readily reproducibleprotocols, and notes on troubleshooting and avoidingknown pitfalls. Authoritative and easily accessible,Carbohydrate Microarrays: Methods and Protocolscompiles a catalogue of protocols on carbohydratemicroarrays to span the needs of researchers aroundthe globe.



▶109,95 €

C.H. Clarke, Department of Translational Research, TheUniversity of Texas, M.D. Anderson Cancer Center, Houston,

TX, USA; D.L.B. McCarthy, Bio-Rad Laboratories, Malvern, PA,USA (Eds.)

SELDI-TOF Mass SpectrometryMethods and Protocols

SELDI is distinct from other TOF-MS technologiesin that it couples features of chromatography andmass spectrometry, facilitating analyte enrichmentand sample cleanup on an array surface. In thegrowing field of proteomics, SELDI technologyhas been widely used for biomarker discoveryand characterization in diverse applicationsincluding diagnostics, drug development, andbasic research. SELDI-based biomarker studies cantypically be divided into four phases: discovery,validation, purification and identification, and assaydevelopment. SELDI-TOF Mass Spectrometry:Methods and Protocols provides an overview of thecurrent applications of SELDI-TOF MS (surfaceenhanced laser desorption/ionization time-of-flight mass spectrometry), with an emphasis onstudy and experimental design, data analysis andinterpretation, and assay development. Written inthe highly successful Methods in Molecular Biology™series format, chapters contain introductions to theirrespective topics, lists of the necessary materialsand reagents, step-by-step, readily reproduciblelaboratory protocols, and notes on troubleshootingand avoiding known pitfalls. Authoritative andaccessible, SELDI-TOF Mass Spectrometry: Methodsand Protocols will provide information on optimizingstudy design, experimental protocols, and dataanalysis and interpretation to yield robust biomarkersand biomarker assays, using examples from differentdisease areas.


2012. X, 239 p. 54 illus., 4 in color. (Methods in MolecularBiology, Vol. 818) Hardcover978-1-61779-417-9

▶94,95 €

R. Cramer, University of Reading, UK; R. Westermeier, SERVAElectrophoresis GmbH, Heidelberg, Germany (Eds.)

Difference Gel Electrophoresis(DIGE)Methods and Protocols

Protein analysis is increasingly becoming acornerstone in deciphering the molecularmechanisms of life. Proteomics, the large-scale andhigh-sensitivity analysis of proteins, is already pivotalto the new life sciences such as Systems Biologyand Systems Medicine. Proteomics, however, reliesheavily on the past and future advances of protein

purification and analysis methods. DIGE, beingable to quantify proteins in their intact form, isone of a few methods that can facilitate this type ofanalysis and still provide the protein isoforms inan MS-compatible state for further identificationand characterization with high analytical sensitivity.Differential Gel Electrophoresis: Methods andProtocols introduces the concept of DIGE andits advantages in quantitative protein analysis. Itprovides detailed protocols and important noteson the practical aspects of DIGE with both genericand specific applications in the various areas ofQuantitative Proteomics. Divided into four concisesections, this detailed volume opens with the basicsof DIGE, the technique and its practical details witha focus on the planning of a DIGE experiment andits data analysis. The next section introduces variousDIGE methods from those employed by scientistsworld-wide to more novel methods, providing aglance at what is on the horizon in the DIGE world. The volume closes with an overview of the widerange of DIGE applications from Clinical Proteomicsto Animal, Plant, and Microbial Proteomicsapplications. Written in the highly successful Methodsin Molecular Biology™ series format, chapterscontain introductions to their respective topics,lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols,and notes on troubleshooting and avoiding knownpitfalls.Authoritative and accessible, Differential GelElectrophoresis: Methods and Protocols can be usedby novices with some background in biochemistry ormolecular biology as well as by experts in Proteomicswho would like to deepen their understanding ofDIGE and its employment in many hyphenationsand application areas. With its many protocols,applications, and methodological variants, it is alsoa unique reference for all who seek fundamentaldetails on the working principle of DIGE and ideasfor possible future uses of DIGE in novel analyticalapproaches.


2012. XIII, 401 p. 73 illus., 34 in color. (Methods in MolecularBiology, Vol. 854) Hardcover978-1-61779-572-5

▶109,95 €

M. De Ley, Katholieke Universiteit Leuven, Heverlee,Belgium (Ed.)

Cytokine ProtocolsRecent studies have discovered new known andcharacterized cytokines, allowing for advancementin miniaturization of micro-analytical methods aswell as the extensive development of bio-informaticsand nanotechnology. These advancements have

http://www.springer.com/978-1-61779-372-1http://www.springer.com/978-1-61779-417-9http://www.springer.com/978-1-61779-572-5

springer.com 7

allowed researchers to reduces sample sizes makingfor more accurate determinations then previouslypossible. In Cytokine Protocols: Second Edition,expert researchers in the field detail many of themethods which are now commonly used to studycytokines. These methods and techniques forstudying cytokines include historical importanceand the importance of researchers using bioassay,quantification, and characterization of cytokinerelated RNAs, posttranscriptional modifications ofRNA, either naturally or artificially, and observationsat the protein level. Written in the highly successfulMethods in Molecular Biology™ series format, thechapters include the kind of detailed descriptionand implementation advice that is crucial for gettingoptimal results in the laboratory. Authoritative andpractical, Cytokine Protocols: Second Edition seeksto aid scientists in furthering the crucially importantadvancement of cytokine research.



▶94,95 €

R.J. Dohmen, University of Cologne, Germany; M. Scheffner,University of Konstanz, Germany (Eds.)

Ubiquitin Family Modifiers andthe ProteasomeReviews and Protocols

The ubiquitin-proteasome system (UPS) andubiquitin-related modifiers are not only involvedin cellular protein quality control but also in theregulation of many fundamental cellular processes/pathways as well as in their disease-relevantaberrations. Ubiquitin Family Modifiers andProteasome: Reviews and Protocols presents bothnovel developments in UPS research and importantmethods related to the main recent advances in thefield of ubiquitin family modifiers. Divided intofive convenient sections, this volume focuses on theenzymology and substrate identification of ubiquitinfamily modifiers, the recognition and chain formationof these modifiers, the analysis of proteasomebiogenesis and function, protein quality control, andfinally the use of small molecules and strategies tostudy or manipulate the function of the UPS and ofubiquitin family modifiers, respectively. Written inthe highly successful Methods in Molecular Biology™series format, chapters include introductions to theirrespective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible protocols,and notes on troubleshooting and avoiding knownpitfalls. Authoritative and easily accessible, Ubiquitin

Family Modifiers and Proteasome: Reviews andProtocols will be of great use to investigators andstudents engaged in both basic and applied researchin life sciences.



▶124,95 €

J.A. Douthwaite, MedImmune Limited, Cambridge, UK; R.H.Jackson, MedImmune Limited, Cambridge, UK (Eds.)

Ribosome Display and RelatedTechnologiesMethods and Protocols

Display technologies have become a very powerfulway of generating therapeutic lead molecules andspecific reagents for increasing our understandingof biology; however, despite being first describedshortly after phage display, the use of ribosomedisplay and related methods have been much lesswidespread. Since this is in part due to the complexityof the methods, Ribosome Display and RelatedTechnologies: Methods and Protocols seeks to extendtheir use by collecting expert contributions describingthese detailed protocols. The protocols describedrange from well-established methods that have beenused for a decade to generate high affinity antibodies,which are already in the clinic, to methods that arein their early stages of application such as displayof peptides incorporating non-canonical aminoacids. Written in the highly successful Methodsin Molecular Biology™ series format, chaptersinclude introductions to their respective topics,lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols,and tips on troubleshooting and avoiding knownpitfalls. Invaluable and easy to use, Ribosome Displayand Related Technologies: Methods and Protocolswill be of great benefit to those with general molecularbiology or protein engineering experience who wishto select peptides or proteins by display, those withphage display experience who would benefit from theapplication of ribosome display, as well as those withsome ribosome display experience who would like toexpand the range of applications to which they areapplying the technology.


2012. XIII, 424 p. 57 illus. (Methods in Molecular Biology, Vol.805) Hardcover978-1-61779-378-3

▶109,95 €

G. Drewes, Cellzome AG, Heidelberg, Germany; M.Bantscheff, Cellzome AG, Heidelberg, Germany (Eds.)

Chemical ProteomicsMethods and Protocols

The multidisciplinary science of chemical proteomicsstudies how small molecules of synthetic or naturalorigin bind to proteins and modulate their function. In Chemical Proteomics: Methods and Protocols,expert researchers in the field provide key techniquesto investigate chemical proteomics focusing onanalytical strategies, how probes are generated,techniques for the discovery of small moleculetargets and the probing of target function, and smallmolecule ligand and drug discovery. Written in thehighly successful Methods in Molecular Biology™series format, chapters include introductions to theirrespective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratoryprotocols, and key tips on troubleshooting andavoiding known pitfalls. Authoritative and practical,Chemical Proteomics : Methods and Protocols seeksto provide methodologies that will contribute to awider application of chemical proteomics methods inbiochemical and cell biological laboratories.



▶94,95 €

J.M. Dunwell, The University of Reading, UK; A.C. Wetten,The University of Reading, UK (Eds.)

Transgenic PlantsMethods and Protocols

In 2010 the global area of transgenic crops reached148 million hectares, an 87-fold increase since 1996,making it the most rapidly adopted technology inthe history of modern agriculture. In TransgenicPlants: Methods and Protocols, Second Edition expertresearchers in the field provide key techniques toinvestigate production and analysis of transgenicplants. Focusing on selection and detection methods,


8 springer.com

transformation technology, gene targeting, silencingand directed mutation, metabolic engineering andpharming, the book encompasses protocols relatingto major crops and model plants being used forgenomic analysis. Written in the highly successfulMethods in Molecular Biology™ series format, thechapters include the kind of detailed descriptionand implementation advice that is crucial forgetting optimal results in the laboratory. Thoroughand intuitive, Transgenic Plants: Methods andProtocols, Second Edition aids scientists in thecontinuous improvements being made for theproduction and analysis of transgenic plants.


2012. XV, 497 p. 92 illus., 30 in color. (Methods in MolecularBiology, Vol. 847) Hardcover978-1-61779-557-2

▶124,95 €

R.C. Elston, Case Western Reserve University, Cleveland,OH, USA; J.M. Satagopan, Memorial Sloan-Kettering CancerCenter, New York, NY, USA; S. Sun, Case Western ReserveUniversity, Cleveland, OH, USA (Eds.)

Statistical Human GeneticsMethods and Protocols

Recent advances in genetics over the last quarterof a century, especially in molecular techniques,have dramatically reduced the cost of determininggenetic markers and hence opened up a field ofresearch that is increasingly helping to detect,prevent and/or cure many diseases that afflicthumans. In Statistical Human Genetics: Methodsand Protocols expert researchers in the field describestatistical methods and computer programs inthe detail necessary to make them more easilyaccessible to the beginner analyzing data. Writtenin the highly successful Methods in MolecularBiology™ series format, with examples of runningthe programs and interpreting the program outputs,the chapters include the kind of detailed descriptionand implementation advice that is crucial for gettingoptimal results from human genetic data collected inthe laboratory. Thorough and as much as possibleintuitive, Statistical Human Genetics: Methodsand Protocols aids scientists in understanding thecomputer programs and analytical procedures theyneed to use.



▶124,95 €

A.W. Fenton, The University of Kansas Medical Center,Kansas City, KS, USA (Ed.)

AllosteryMethods and Protocols

Despite considerable variability within the scientificcommunity, allosteric regulation can best be definedfunctionally as how a macromolecule binds oneligand differently when a second ligand is or is notpre-bound to the macromolecule, which constitutesa vital aspect of protein structure/function. InAllostery: Methods and Protocols, expert researchersin the field provide key techniques to investigate thisbiological phenomenon. Focusing on heterotropicsystems with some coverage of homotropic systems,this volume covers the monitoring of allostericfunction, allosteric conformational changes,and allosteric changes in protein dynamics/sub-population distribution, as well as topics suchas macromolecular and ligand engineering ofallosteric functions and computational aids in thestudy of allostery. Written in the highly successfulMethods in Molecular Biology™ series format, thechapters include the kind of detailed descriptionand implementation advice that is crucial for gettingoptimal results in the laboratory. Thorough andintuitive, Allostery: Methods and Protocols aidsscientists in continuing to study ligand-induced,through-protein effects on protein function (ligandbinding/catalysis), a phenomenon that is wellrecognized through the history of the life sciences andvery poorly understood at the molecular level.


2012. XVIII, 439 p. 134 illus. (Methods in Molecular Biology,Vol. 796) Hardcover978-1-61779-333-2

▶109,95 €

H.J. Fromm, Iowa State University, Ames, IO, USA; M.Hargrove, Iowa State University, Ames, IO, USA

Essentials of BiochemistryThis textbook, Essentials of Biochemistry is aimed atchemistry and biochemistry undergraduate studentsand first year biochemistry graduate students. Itincorporates the lectures of the authors given tostudents with a strong chemistry background. Anemphasis is placed on metabolism and reactionmechanisms and how they are studied. As the titleof the book implies, the text lays the basis for anunderstanding of the fundamentals of biochemistry.

More on www.springer.com/978-3-642-19623-2Online orders shipping within 2-3 days.

2012. XVIII, 364 p. 349 illus., 209 in color. Hardcover978-3-642-19623-2

▶49,95 €

J.M. Gibbins, Institute for Cardiovascular and MetabolicResearch, School of Biological Sciences, University ofReading, Reading, Berkshire, UK; M.P. Mahaut-Smith,Department of Cell Physiology and Pharmacology,University of Leicester, Leicester, UK (Eds.)

Platelets and MegakaryocytesVolume 3, Additional Protocols and Perspectives

New techniques to study cell signaling and functioncan develop at a staggering pace; however, manyapproaches are as valid today as on the day theywere established. Thus, the main aim of Platelets andMegakaryocytes: Volume 3, Additional Protocolsand Perspectives is to complement the first twovolumes published in 2004 by adding recentlydeveloped state of the art techniques. Convenientlydivided into three sections, this detailed volumecovers techniques to study platelet function,approaches to investigate megakaryocyte function,and perspectives on important overall concepts in thefield of megakaryocyte and platelet biology. Writtenin the highly successful Methods in MolecularBiology™ series format, methods chapters includeintroductions to their respective topics, lists of thenecessary materials and reagents, step-by-step,


springer.com 9

readily reproducible laboratory protocols, andvital tips on troubleshooting and avoiding knownpitfalls. Authoritative and up-to-date, Platelets andMegakaryocytes: Volume 3, Additional Protocols andPerspectives adds a wealth of new expertise for thelabs of scientists working in this key biological area ofstudy.



▶109,95 €

G.S. Hall, Cleveland Clinic Foundation, Cleveland, OH, USA(Ed.)

Interactions of Yeasts, Moulds,and Antifungal AgentsHow to Detect Resistance

The incidence of fungal infections increases withthe increase in antibiotic usage and increasingimmunosuppressed populations. There is no longeronly one antifungal agent and the response of fungito various agents is not always predictable. The needfor standardized antifungal susceptibility testing, andstandardized interpretation of results in conjunctionwith studies that describe clinical outcomes based onthose tools is ever important. Interactions of Yeasts,Moulds, and Antifungal Agents: How to DetectResistance covers the available antifungal agents, howto perform in vitro testing and how those resultsshould be interpreted for the most common fungalpathogens.


2012. XIII, 168 p. 2 illus. in color. Hardcover978-1-58829-847-8

▶99,95 €

P.C. Hallenbeck, Université de Montréal, QC, Canada (Ed.)

Microbial Technologies inAdvanced Biofuels ProductionConcerns over dwindling fossil fuel reserves andimpending climate changes have focused attentionworldwide on the need to discover alternative,sustainable energy sources and fuels. Biofuels, alreadyproduced on a massive industrial scale, are seen asone answer to these problems. However, very realconcerns over the effects of biofuel production onfood supplies, with some of the recent increasesin worldwide food costs attributable to biofuelproduction, have lead to the realization that new,non-food substrates for biofuel production mustbe bought online. This book is an authoritative,comprehensive, up-to-date review of the variousoptions under development for the production ofadvanced biofuels as alternative energy carriers.Accomplished, internationally recognized expertscarrying out research on individual focus areascontribute specific technical chapters detailingpresent progress and future prospects. A generaloverview places the field in context, and a finalchapter overviews some of the latest developments inthis field.


2012. X, 272 p. 39 illus., 16 in color. Hardcover978-1-4614-1207-6

▶139,95 €

N.W. Hardy, Aberystwyth University, UK; R.D. Hall, PlantResearch International, Wageningen, Netherlands (Eds.)

Plant MetabolomicsMethods and Protocols

Estimation of the metabolite complement of plantmaterial involves a wide range of techniques andtechnologies and that breadth continues to increase. Metabolomics research typically involves multiplesites for material preparation and analysis and mostinvestigations are “high throughput”, meaningthat chemical analysis of sample sets are inevitably

carried out over an extended period of time. In,Plant Metabolomics: Methods and Protocols expertresearchers in the field detail many of the stageswhich are now commonly used to study plantmetabolomics workflow. Stages of this workflow,up to and including the statistical analysis, accurateand detailed collection of meta-data are also essentialfor good process management, to satisfy reportingrequirements and to ensure wider interpretabilityand reuse results.Written in the highly successfulMethods in Molecular Biology™ series format,chapters include introductions to their respectivetopics, lists of the necessary materials and reagents,step-by-step, readily reproducible laboratoryprotocols, and key tips on troubleshooting andavoiding known pitfalls. Through and intuitivePlant Metabolomics: Methods and Protocols, seeks toaid scientists in the further study of the methods forall the stages of the plant metabolomics workflow.


2012. XIII, 340 p. 57 illus., 2 in color. (Methods in MolecularBiology, Vol. 860) Hardcover978-1-61779-593-0

▶109,95 €

J.S. Hartig, University of Konstanz, Germany (Ed.)

RibozymesMethods and Protocols

Over the past decade, there have been a variety offundamental insights into ribozymes which havechanged the perspective on RNA catalysis. In,Ribozymes: Methods and Protocols, researchers inthe field detail many of the protocols and techniqueswhich are now commonly used to study individualribozymes and the characterization of ribozymes.Written in the highly successful Methods inMolecular Biology™ series format, chapters includeintroductions to their respective topics, lists of thenecessary materials and reagents, step-by-step,readily reproducible laboratory protocols, andkey tips on troubleshooting and avoiding knownpitfalls. Authoritative and practical, Ribozymes:Methods and Protocols seeks to aid scientists infurther understanding the catalytically active RNAs .


2012. XIV, 480 p. 102 illus., 27 in color. (Methods inMolecular Biology, Vol. 848) Hardcover978-1-61779-544-2

▶109,95 €

http://www.springer.com/978-1-61779-306-6http://www.springer.com/978-1-58829-847-8http://www.springer.com/978-1-4614-1207-6http://www.springer.com/978-1-61779-593-0http://www.springer.com/978-1-61779-544-2

10 springer.com

D.L. Hatfield, National Institutes of Health, Bethesda, MD,USA; M.J. Berry, University of Hawaii at Manoa, Honolulu, HI,USA; V.N. Gladyshev, Harvard Medical School, Boston, MA,USA (Eds.)

SeleniumIts Molecular Biology and Role in Human Health

The selenium field has grown dramatically in theyears since the first edition of Selenium: Its MolecularBiology and Role in Human Health was published in2001. All aspects of selenium biology have advancedwith many new approaches and insights into thebiochemical, molecular, genetic, and health areasof this intriguing element. The third edition ofSelenium: Its Molecular Biology and Role in HumanHealth brings readers up to date and informs themof the present knowledge of the molecular biologyof selenium, its incorporation into proteins asselenocysteine, and the role that this element andselenium-containing proteins (selenoproteins) play inhealth and development. The current edition will bean important resource for scientists and investigatorsin the selenium field, students, and physicianswho wish to learn more about this fascinatingmicronutrient.


2012. XXV, 598 p. 106 illus., 37 in color. Hardcover978-1-4614-1024-9

▶129,95 €

J. van Helden, Université Libre, Bruxelles, Belgium; A.Toussaint, Université Libre, Bruxelles, Belgium; D. Thieffry,TAGC - INSERM U928 & Université de la Méditerranée,Marseille, France (Eds.)

Bacterial Molecular NetworksMethods and Protocols

Network-based representations have becomepervasive in most fields in biology. BacterialMolecular Networks: Methods and Protocols providesauthoritative descriptions of various experimental andcomputational methods enabling the characterizationand analysis of molecular interaction networks, witha focus on bacteria. Divided into three convenient

sections, this volume provides extensive coverage of various experimental and in silico approaches aimingat the characterization of network components,addresses the presentation of computationalapproaches to analyze the topology of molecularnetworks, and further introduces a variety of methodsand tools enabling scientists to generate qualitative orquantitative dynamical models of molecular processesin bacteria. Written in the highly successful Methodsin Molecular Biology™ series format, chapters includeintroductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readilyreproducible protocols, and notes on troubleshootingand avoiding known pitfalls. Authoritative andaccessible, Bacterial Molecular Networks: Methodsand Protocols is intended primarily for post-graduatestudents and researchers working in the field ofexperimental and computational microbiology andprovides a combination of up-to-date reviews alongwith detailed protocols written by the developersof bioinformatics resources, such as databases andsoftware tools.



▶124,95 €

M.H. Helfrich, University of Aberdeen, UK; S.H. Ralston,University of Edinburgh, UK (Eds.)

Bone Research ProtocolsStudies over the past decade have continued to bringtremendous advances to our understanding of bonebiology. New pathways have been discovered andexpanded our knowledge of the ways in which genesand gene products affect bone cells and therebybone mass and bone strength. In Bone ResearchProtocols, Second Edition, expert researchers in thefield detail many methods commonly used to studybone biology. Focusing mainly on in vitro methods,this volume gives techniques for isolation, cultureand functional analysis of all bone cell types anddetails a range of imaging methods, including lightand ultrastructural microscopy and live cell imaging.Some important in vivo techniques are included, suchas analysis of bone resorption and imaging using Xrays, fluorescent or luminescent techniques. Methodsfor study of proteins and nucleic acid are includedand methods for analysis of bone composition,measurement of bone strength, and response tomechanical stimulation are described. Written inthe highly successful Methods in Molecular Biology™series format, chapters include introductions to theirrespective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory

protocols, and key tips on troubleshooting andavoiding known pitfalls. Authoritative and practical,Bone Research Protocols, Second Edition seeks to aidscientists in the bone field to establish new techniquesin their laboratories.



▶124,95 €

International Association for Food Protection, Des Moines,USA

Procedures to InvestigateFoodborne IllnessProcedures to Investigate Foodborne Illness isdesigned to guide public health personnel orteams in any country that investigates reports ofalleged foodborne illnesses. The manual is basedon epidemiologic principles and investigativetechniques that have been found effective indetermining causal factors of disease incidence.The guidelines are presented in the sequenceusually followed during investigations and areorganized so that an investigator can easilyfind the information needed in any phase of aninvestigation. Included are descriptions of thefollowing procedures:Plan, prepare, investigateand respond to intentional contaminationof foodHandle illness alerts and food-relatedcomplaints that may be related to illnessInterviewill persons, those at risk, and controlsDevelop acase definitionCollect and ship specimens and foodsamplesConduct hazard analysis (environmentalassessments) at sites where foods responsiblefor outbreaks were produced, processed, orpreparedTrace sources of contaminationIdentifyfactors responsible for contamination, survival ofpathogenic microorganisms or toxic substances,and/or propagation of pathogensCollate andinterpret collected dataReport information aboutthe outbreakThis edition also contains extensivelyupdated and more user-friendly keys to assistinvestigators in identifying the contributing factorsthat may lead to the contamination, proliferation orsurvival of agents of foodborne disease.


2011. X, 167 p. 47 illus., 20 in color. Softcover978-1-4419-8395-4

▶19,95 €


springer.com 11

International Commission on Microbiological Specificationsfor Foods (ICMSF)

Microorganisms in Foods 8Use of Data for Assessing Process Control andProduct Acceptance

Continuing the ICMSF series, Microorganismsin Foods 8 provides practical guidance onappropriate testing of food processing environments,processing lines, shelf life and finished product toenhance the safety and microbiological quality ofthe food supply. Part I, Principles of Using Data inMicrobial Control, summarizes the principles ofMicroorganisms in Foods 7: MicrobiologicalTesting in Food Safety Management (2002),which illustrates how HACCP and Good HygienicPractices (GHP) provide greater assurance of safetythan microbiological testing, and also identifiescircumstances where microbiological testingmay play a useful role. Part II, Applications ofPrinciples to Product Categories, updates andexpands Part II of Microorganisms in Foods 2:Sampling for Microbiological Analysis: Principlesand Specific Applications (1986) and builds onMicroorganisms in Foods 6: Microbial Ecologyof Food Commodities (2nd edition, 2005) withrecommended tests and specific criteria for 19 foodproduct categories to evaluate the effectivenessof controls.

Microorganisms in Foods 8 isintended for anyone using microbiological testing orengaged in setting microbiological criteria includinggovernment, food processors and the customers theysupply. This book provides actionable informationfor food quality assurance professionals, foodmicrobiologists, food technologist, veterinarians,public health workers and regulatory officials. Thispeer reviewed book was prepared by the InternationalCommission on Microbiological Specificationsfor Foods (ICMSF), with assistance from a limitednumber of consultants. The ICMSF was formed inresponse to the need for internationally acceptableand authoritative guidance on microbiological limitsfor foods in international commerce. Currently themembership consists of 17 food microbiologists andpublic health professionals from eleven countries,drawn from government, universities, and foodprocessing and related industries.

Alsoavailable

Microorganisms in Foods 7:Microbiological Testing in Food Safety Management,2002 (978-0-306-47262-6)

Microorganismsin Foods 6: Microbial Ecology ofFood Commodities, 2nd edition 2005(978-1-4419-3465-9)

Microorganisms in Foods

5: Characteristics of Microbial Pathogens, 1996(978-0-412-47350-0)


2011. XX, 400 p. 12 illus. Hardcover978-1-4419-9373-1

▶169,95 €

S. Lindström, POOLIA Sweden, Stockholm, Sweden; H.Andersson-Svahn, Royal Institute of Technology, Stockholm,Sweden (Eds.)

Single-Cell AnalysisMethods and Protocols

Today, cells are commonly analyzed en masse, withthousands of cells per sample, yielding results onthe average response of the cells. However, cellularheterogeneity implies that in order to learn moreabout cellular behaviour, it is important to studyhow individual cells respond, one by one. In Single-Cell Analysis: Methods and Protocols, experts inthe field provide an update on the field of single-cellanalysis wherein the latest findings and applicationsare described in detail. The methods described inthis book include a few examples of conventionalmethods and several examples of miniaturizedmethods. Written in the highly successful Methodsin Molecular Biology™ series format, chapters includeintroductions to their respective topics, lists of thenecessary materials and reagents, step-by-step,readily reproducible laboratory protocols, andkey tips on troubleshooting and avoiding knownpitfalls. Authoritative and accessible, Single-CellAnalysis: Methods and Protocols encourages readersto explore new ways of studying cells that may helplead to exciting new discoveries.



▶109,95 €

C. López-Larrea, Hospital Universitario Central de Asturias,Oviedo, Spain; A. López Vázquez, Hospital UniversitarioCentral de Asturias, Oviedo, Spain; B. Suárez Álvarez,Hospital Universitario Central de Asturias, Oviedo, Spain(Eds.)

Stem Cell Transplantation.

More on www.springer.com/978-1-4614-2097-2Due: June 28, 2012

2012. XXVI, 340 p. 48 illus., 17 in color. (Advances inExperimental Medicine and Biology, Vol. 741) Hardcover978-1-4614-2097-2

▶149,95 €

B. Mallick, New York State Department of Health, NY, USA; Z.Ghosh, Bose Institute, Kolkata, India (Eds.)

Regulatory RNAsBasics, Methods and Applications

Recent progress in high-throughput technologiesand genome wide transcriptome studies have leadto a significant scientific milestone of discoveringnon-coding RNAs (ncRNAs) which spans througha major portion of the genome. These RNAs mostoften act as riboregulators, and actively participatein the regulation of important cellular functions atthe transcriptional and/or post-transcriptional levelsrather than simply being an intermediated messengerbetween DNA and proteins. As the appreciation forthe importance of ncRNAs continues to emerge, it isalso increasingly clear that these play critical roles ingene regulatory processes during development anddifferentiation. Further, regulatory RNAs are usefulbiomarkers for diagnosis of diseases. Hence theseRNA regulators are essential to the development oftherapeutics. This book on “Regulatory RNAs” offersa comprehensive view on our current understandingof these regulatory RNAs viz. siRNA, miRNA, piRNA,snoRNA, long non-coding RNA, small RNA etc.It addresses both the biogenesis and mechanismof action of regulatory RNAs with a primary focuson their annotation, experimental methodologies(microarray, next-gen sequencing etc.) for their


12 springer.com

discovery, computational tools for their prediction,and above all, applications of these revolutionaryregulatory molecules in understanding biologicalsystems and diseases, including therapeutics. Thiscomprehensive volume is intended for readers withresearch or teaching interests in ncRNA biologyand will provide a major information resource oncurrent research in the fast-moving fields of RNAand gene expression regulation. Cutting-edge andconcise, “Regulatory RNAs: Basics, Methods andApplications” promises to support vital research inthe field of regulatory RNAs, ever-continuing to growrapidly and gain increasing importance in basic andtranslational biology.


2012. IX, 552 p. 65 illus., 37 in color. Hardcover978-3-642-22516-1

▶149,95 €

B. Marcus, Genesee Community College, Batavia, NY, USA

Evolution That Anyone CanUnderstand.


2012. XII, 116 p. 6 illus., 1 in color. (SpringerBriefs inEvolutionary Biology) Softcover978-1-4419-6125-9

▶49,95 €

M.A. McGuckin, Mater Medical Research Institute, SouthBrisbane, QLD, Australia; D.J. Thornton, University ofManchester, UK (Eds.)

MucinsMethods and Protocols

Epithelial mucins are large complex cell surface andsecreted glycoproteins produced by mucosal epithelialcells. In, Mucins: Methods and Protocols expert

researchers in the field detail many of the methodswhich are now commonly used to study Mucins.These include methods and techniques for the bestapproaches to analysing each specific area of mucinbiochemistry, physiology and biophysics beforeproviding individual detailed experimental protocolstogether with troubleshooting and interpretationtips. Written in the highly successful Methods inMolecular Biology™ series format, chapters includeintroductions to their respective topics, lists of thenecessary materials and reagents, step-by-step,readily reproducible laboratory protocols, andkey tips on troubleshooting and avoiding knownpitfalls. Authoritative and practical, Mucins: Methodsand Protocols is designed to be a useful resourcefor those entering the mucin field and to facilitatethose already studying mucins to broaden theirexperimental approaches to understanding mucosalbiology.



▶109,95 €

R.R. Mitry, King's College Hospital, London, UK; R.D. Hughes,King's College, London, UK (Eds.)

Human Cell Culture ProtocolsHuman cell culture is not a new topic, but thedevelopment of new molecular techniques andreagents which can be used to investigate cell functionand the responsible intracellular mechanisms makeit a continuing requirement. This third edition ofHuman Cell Culture Protocols expands upon theprevious editions with current, detailed protocolsfor the isolation and culture of a range of primarycells from human tissues. With new chapters onpancreatic cells needed for basic studies on thepathogenesis of diabetes and for their applicationfor islet transplantation, the book also delves intoprotocols for hepatocytes, skin cells, lung cells,parathyroid cells, gastric cells, renal cells, adipocytes,ovarian cells, bone cells, vascular smooth musclecells, vascular endothelial cells, regulatory T cells,blood mononuclear cells, as well as new techniquesbeing applied to human cell culture, particularly theuse of biocompatible scaffolds to grow cells, the invitro use of laser microdissection to isolate cells fromculture, and automated cell culture. Written in thehighly successful Methods in Molecular Biology™series format, chapters include introductions to theirrespective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoidingknown pitfalls. Authoritative and cutting-edge,

Human Cell Culture Protocols, Third Edition makesit possible for a worker with basic cell culture training,whether in the fields of cell biology, gene therapy,and cell transplantation, to prepare cell cultures ofthe specific cell type necessary to forward their vitalresearch.



▶109,95 €

T. Ochiya, National Cancer Center Research Institute, Tokyo,Japan (Ed.)

Liver Stem CellsMethods and Protocols

Increasing evidence suggests that liver stem cellshave the capacity to differentiate into parenchymalhepatocytes or into bile ductular cells. These stemcells may be activated to proliferate after severe liverinjury or exposure to hepatocarcinogens. Stem cellreplacement strategies are being investigated as analternative approach to liver repair and regeneration.Additionally, stem cell transplantation has beenshown to significantly improve liver function andincrease survival in experimentally-induced liver-injury models in animals. In Liver Stem Cells:Methods and Protocols, expert researchers focus on several hepatic progenitor cells, hepatic differentiationform stem cells, bile ductal cell formation from stemcells, liver stem cells and hepatocarcinogenesi, and application of liver stem cells for cell therapy. Thesetopics shed light on stem cell technology whichmay lead to the development of effective clinicalmodalities for human liver diseases. Written in thehighly successful Methods in Molecular Biology™series format, chapters include introductions to theirrespective topics, lists of the necessary materialsand reagents, step-by-step, readily reproduciblelaboratory protocols, and key tips on troubleshootingand avoiding known pitfalls. Thorough and intuitive,Liver Stem Cells: Methods and Protocols seeks toaid scientists in the further study of preclinical andclinical investigations that explore the therapeuticpotential of stem cells in repair of liver injuries.



▶94,95 €

http://www.springer.com/978-3-642-22516-1http://www.springer.com/978-1-4419-6125-9http://www.springer.com/978-1-61779-512-1http://www.springer.com/978-1-61779-366-0http://www.springer.com/978-1-61779-467-4

springer.com 13

A.J.W. Orry, MolSoft LLC, La Jolla, CA, USA; R. Abagyan,University of California, San Diego, La Jolla, CA, USA (Eds.)

Homology ModelingMethods and Protocols

Knowledge about protein tertiary structure canguide experiments, assist in the understanding ofstructure-function relationships, and aid the designof new therapeutics for disease. Homology modelingis an in silico method that predicts the tertiarystructure of an amino acid sequence based on ahomologous experimentally determined structure. In, Homology Modeling: Methods and Protocolsexperts in the field describe each homology modelingstep from first principles, provide case studies forchallenging modeling targets and describe methodsfor the prediction of how other molecules suchas drugs can interact with the protein. Writtenin the highly successful Methods in MolecularBiology™ series format, the chapters include thekind of detailed description and implementationadvice that is crucial for getting optimal results inthe laboratory. Thorough and intuitive, HomologyModeling: Methods and Protocols guides scientists inthe available homology modeling methods. .



▶109,95 €

C.M. Palmeira, University of Coimbra, Portugal; A.J. Moreno,University of Coimbra, Portugal (Eds.)

Mitochondrial BioenergeticsMethods and Protocols

Increasing interest in mitochondrial bioenergetics isbeing driven by the impact of drug and environmentalchemical-induced disturbances of mitochondrialfunction as well as hereditary deficiencies andthe progressive deterioration of bioenergeticperformance with age. These initiatives have fosteredthe investigation of genetic and environmentalinfluences on bioenergetics. In Mitochondrial

Bioenergetics: Methods and Protocols, researchersin the field detail the practical principles and assaysdesigned to derive quantitative assessment of eachset of parameters that reflect different aspects ofmitochondrial bioenergetics. Written in the highlysuccessful Methods in Molecular Biology™ seriesformat, chapters include introductions to theirrespective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratoryprotocols, and key tips on troubleshooting andavoiding known pitfalls Authoritative and practical,Mitochondrial Bioenergetics: Methods and Protocolshelps elevate the quality and rate of investigativediscoveries regarding disease states associated withenvironmental or genetic influences on mitochondrialbioenergetics.



▶94,95 €

F. Rivero, University of Hull, UK (Ed.)

Rho GTPasesMethods and Protocols

Although initially described as major regulatorsof cytoskeletal remodeling, Rho GTPases havebeen implicated in the establishment of polarity,endocytosis, vesicle trafficking, morphogenesis,cytokinesis, transcriptional activation, cell cycleprogression, and apoptosis, to mention a few. Inaddition, Rho GTPases have acquired medicalrelevance because of their participation intumorigenesis and metastasis, in cardiovascularconditions, and as targets of infectious agents. Thefield has broadened even more with the contributionof studies in model organisms (plants, amoebas,fungi, invertebrates), each adding a particularview to the complexity of the family and vastlyenriching our perception of these important signalingcomponents. Divided into five convenient sections,Rho GTPase: Methods and Protocols provides anhistorical overview of the field and an account of thephylogenetics of the Rho family, general biochemicalmethods, and functional assays that allow monitoringthe consequences of manipulating Rho GTPases in avariety of contexts. Additionally, the volume devotes asection to advanced imaging methods and to recentlydeveloped high throughput methods, closing withtechniques specifically designed for studies in selectednon-mammalian model organisms. Written in thesuccessful Methods in Molecular Biology™ seriesformat, chapters include introductions to theirrespective topics, lists of the necessary materials and

reagents, step-by-step, readily reproducible protocols,and notes on troubleshooting and avoiding knownpitfalls. Authoritative and easily accessible, RhoGTPase: Methods and Protocols provides techniquesthat are standard for researchers in the field butalso includes protocols for those who, being alreadyfamiliar with some of the techniques, wish to exploreadditional aspects.



▶109,95 €

B.M. Rothschild, University of Kansas, Lawrence, KS, USA;H.-P. Schultze, University of Kansas, Lawrence, KS, USA; R.Pellegrini, Northeast Ohio Medical University, Rootstown,OH, USA

Herpetological OsteopathologyAnnotated Bibliography of Amphibians andReptiles

Sir William Osler, a famous 19th century physician,observed that diagnosis without knowing theliterature is like going to sea without charts. Thisbook provides the database for identifying the stateof the art/knowledge related to herpetological speciesand diagnoses. It identifies what has been clearlydocumented and areas for future investigation,providing guidance to epidemiology as well as toisolated occurrences. It identifies herpetologicaldiseases which appear to represent potential modelsof human disease, as well as human diseases whichappear to model those identified in amphibiansand reptiles. The interdisciplinary approachof Herpetological Osteopathology: AnnotatedBibliography of Amphibians and Reptiles, includingboth veterinary and human medicine, zoology andpaleontology, provides the literary backgroundfor understanding diseases of bones and otherhard tissues. The book represents a library formacroscopic osseous manifestations of the spectrumof diseases that have been recognized or suspectedin reptiles and amphibians. Definitions of diseasesand abnormalities are provided, followed by anoverview of current understanding of the occurrenceof pathologies in amphibians and reptiles. The mainbody of the book presents the literature from Aristotleto Zwart. The citations are annotated to identifyrecognized pathology, as well as changes in accepteddiagnoses and taxonomic classification.


2012. XI, 450 p. 90 illus., 78 in color. Hardcover978-1-4614-0823-9


14 springer.com

▶149,95 €

F. Rédini, INSERM, Nantes, France (Ed.)

ProteoglycansMethods and Protocols

Now recognized as a reservoir for growth factorsand cytokines modulating cell activation statusand turnover, proteoglycans have stimulated greatamount of interest and research. In Proteoglycans:Methods and Protocols, experts in the field examineissues of basic concepts and up-to-date analysismethods for proteoglycan and glycosaminoglycan(GAG) at the protein and saccharide levels. Thevolume continues with the multifunctional aspectof proteoglycans, highlighted through three relevantexamples of proteoglycans with highly differentstrucutures: serglycin, aggrecan, and heparinsulphate proteoglycans. The final chapter describesproteoglycan involvement in the pathogenesis ofvarious disorders and their potential therapeuticvalue in osteo-articular diseases. Written in thehighly successful Methods in Molecular Biology™series format, chapters include introductions to theirrespective topics, lists of the necessary materialsand reagents, step-by-step, readily reproduciblelaboratory protocols, and tips on troubleshooting andavoiding known pitfalls. Authoritative and accessible,Proteoglycans: Methods and Protocols is an idealguide for scientists attempting to pursue furtherresearch in this vital and complex area of study.



▶109,95 €

A. Shekhtman, State University of New York, Albany, NY,USA; D.S. Burz, State University of New York, Albany, NY,USA (Eds.)

Protein NMR TechniquesThe field of protein NMR spectroscopy has rapidlyexpanded into new areas of biochemistry, molecularbiology and cell biology research that were impossibleto study as recently as ten years ago. This thirdedition of Protein NMR Techniques, expandsupon the previous editions with current, detailedauthoritative but down-to-earth descriptions of newmethodologies. These include techniques for NMRsample preparation, solution and solid state NMRmethodologies and data processing. Written in thehighly successful Methods in Molecular Biology™series format, chapters include introductions to theirrespective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratoryprotocols, and key tips on troubleshooting andavoiding known pitfalls. Authoritative and practical,Protein NMR Techniques, Third Edition, seeks to aidscientists in understanding the latest innovations inthe field of protein NMR.



▶124,95 €

E.M. Sigurdsson, New York University School of Medicine,NY, USA; M. Calero, Instituto de Salud Carlos III, Madrid,Spain; M. Gasset, Consejo Superior de InvestigacionesCientificas, Madrid, Spain (Eds.)

Amyloid ProteinsMethods and Protocols

Amyloid diseases are characterized by the depositionof insoluble fibrous amyloid proteins. The word“amyloid” indicates a starch-like compound, andthough a misnomer, continues to be the acceptedterm for this group of protein conformationaldisorders. The second edition of Amyloid Proteinsexpands upon the previous edition with current,

detailed protocols for the preparation of amyloidand its precursors, specific analytical methods forstudying these proteins, cell culture models and assaysfor production of amyloid proteins, and protocolsfor amyloid extraction from tissue, its detection invitro and in vivo, as well as nontransgenic methodsfor developing amyloid mouse models. Written inthe highly successful Methods in Molecular Biology™series format, chapters include introductions to theirrespective topics, lists of the necessary materialsand reagents, step-by-step, readily reproduciblelaboratory protocols, and key tips on troubleshootingand avoiding known pitfalls.Authoritative andpractical, Amyloid Proteins, Second Edition seeksto aid scientists in the amyloid field to establish newtechniques in their laboratories.Authoritative andpractical, Amyloid Proteins, Second Edition seeksto aid scientists in the amyloid field to establish newtechniques in their laboratories.


2012. XV, 548 p. 115 illus., 46 in color. (Methods in MolecularBiology, Vol. 849) Hardcover978-1-61779-550-3

▶124,95 €

M.J. Somers, University of Pretoria, South Africa; M.Hayward, Australian Wildlife Conservancy, Nichol's Point,VIC, Australia (Eds.)

Fencing for ConservationRestriction of Evolutionary Potential or a Riposteto Threatening Processes?

How can we best protect the world’s biodiversityin the face of the growing human population? Thisquestion is the central theme of contemporaryconservation biology. The protection of biodiversityfrom overuse by fencing it off from the surroundinglandscape is one of the conservation tools availableto us. Fences have become common features of ourenvironment and firmly entrenched in our lives,as we use them to surround our farms, houses, andanything we want to keep to ourselves or protect.Within the last few hundred years, conservationistshave started using fences to protect biodiversity fromoveruse and poaching, as well as to protect peoplefrom wild animals, especially large carnivores andmegaherbivores such as elephants and rhinos. Themethodologies for, and approaches to, the use offences by conservationists vary, and range from theintensive fencing practices in places such as SouthAfrica, to the complete avoidance of fences in otherplaces such as parts of East Africa. In other areassuch as Australia and New Zealand fences are used,at enormous costs, not to protect biodiversity frompeople, but to protect vulnerable native species


springer.com 15

from invasive alien species such as foxes and cats.Some may argue that biodiversity conservation isnot possible without fences, while others argue thatthe fencing in of biodiversity simply creates zoosand restricts evolutionary potential. Fencing forConservation: Restriction of Evolutionary Potentialor a Riposte to Threatening Processes? reviews someof the issues regarding fencing for conservationand summarises

Documents

LIFE SCIENCES - Springer · 2012. 4. 27. · (mulberry, pea, Indian mustard) under environmental stress. Prof. M.N.V. Prasad is a Professor in the Department of Plant Sciences at