LIFE Project Number LIFE13 ENV GR/000414 Inception Report … · The 1st Steering Committee (SC) took place on 29th and 30th of September 2014, Hotel Chandris in the island of Chios

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LIFE13 ENV/GR/000414

LIFE Project Number

LIFE13 ENV GR/000414

Inception Report Covering the project activities from 1/6/2014 to 28/02/2015

Reporting Date

<19/6/2015>

LIFE+ PROJECT NAME or Acronym

LIFE BIODELEAR

Data Project

Project location GREECE

Project start date: 1/06/2014

Project end date: 1/06/2019 Extension date:-

Total budget 2205454 €

EC contribution: 1102727 €

(%) of eligible costs 50%

Data Beneficiary

Name Beneficiary Hellenic Agricultural Organization DEMETER

Contact person Dr Vassilis Mavraganis

Postal address Sof. Venizelou, 1, GR, 14123, Likovrisi

Telephone 30 210 2832031

Fax: 30 210 2842129

E-mail [email protected]; [email protected]

Project Website www.biodelear.gr

mailto:[email protected]

mailto:[email protected]

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1. TABLE OF CONTENTS 1. TABLE OF CONTENTS 2

2. LISTOF ABBREVIATIONS 2

3. EXECUTIVESUMMARY 3

4. ADMINISTRATIVE PART 4

4.1. DESCRIPTION OF PROJECT MANAGEMENT 4

4.2. ORGANIGRAMME OF THE PROJECT TEAMS ANDTHE PROJECT MANAGEMENT

STRUCTURE 8

5. TECHNICAL PART 10

5.1 ACTIONS 11

5.1.1. ACTION A1: PRODUCTION OF TRAPS AND ATTRACTANTS 11

5.1.2. ACTION A2: PREPARATORY MONITORING OF THE DEMONSTRATION AREAS 13

5.1.3. ACTION B1: IMPLEMENTATION OF INNOVATIVE MED TRAPS AT PILOT SCALE 15

5.1.4. ACTION B2: IMPLEMENTATION OF INNOVATIVE MED TRAPS AT REAL SCALE 15

5.1.5. ACTION C1: MONITORING OF PROJECTS ENVIRONMENTAL IMPACT 16

5.1.6. ACTION C2: MONITORING OF PROJECT‟ S SOCIAL-ECONOMICAL IMPACT 16

5.1.7. ACTION D1: DISSEMINATION 17

5.1.8. ACTION D2: “AFTER-LIFE+ COMMUNICATION PLAN” 17

5.1.9. ACTION E1: PROJECT MANAGEMENT BY SSIA 17

5.1.10. ACTION E2: PROJECT MONITORING 18

5.2. AVAILABILITY OF APPROPIATE LICENCES AND AUTHORIZATION 18 5.3. ENVISAGED PROGRESS UNTIL NEXT REPORT 18

6. FINANCIAL 21

7. ANNEXES 23

Annex 1 Photos and the agenda of the 1st Kick-off meeting 23

Annex 2 Photos and the 1st Steering Committee (SC) meeting 26

Annex 3 Photos and the 1st Technical –Monitoring Committee (T-MC) Meeting 30

Annex 4 List of all personnel working on the project with their qualifications and their

role in the project 32

Annex 5 Preliminary Results, Analysis and Conclusion and Table of all soil

parameters measured and the methods of analysis (Action A2) Coordinating

beneficiary (SSIA) responsible (Attached).

Annex 6 Preliminary Results and Conclusions from the analysis of the fruit samplings.

(Action A2) Associated beneficiary (BPI) responsible

Annex 7 Final results and Conclusion of the two efficacy tests (Attached).

Annex 8 Preliminary Results and conclusions on Monitoring of the three fields for

measurement of infestation percentage and determination of the number of

medfly population (Attached).

Annex 9 Permits

1. Permit to use the citrus orchards for three years with the ability to revive it for

other three years (Attached).

2. Permit not to destroy the crop production of citrus fruits (Attached).

Annex 10 Partnership Agreements.

1. Partnership Agreement: Between ELGO-DEMETER and AUTH (Aristotle

University of Thessaloniki) (Attached)

2. Partnership Agreement: Between ELGO-DEMETER and UTH (University of

Thessaly) (Attached)

3. Partnership Agreement: Between ELGO-DEMETER and BPI (Benaki

Phytopathological Institute) (Attached)

Annex 11 Photocopies of the participation in the meetings of the leaders of the

beneficiaries (Attached)

Annex 12 Output Indicators (Attached)

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Deliverable Products of the Project 41

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2. LIST OF ABBREVIATIONS

DC: Dissemination Committee

ELGO DEMETER: Hellenic Agricultural Organisation-DEMETER

SSIA: Soil Science Institute of Athens (Coordinating Beneficiary)

SC: Steering Committee

T-MC: Technical-Monitoring Committee

AUTH: Aristotle University of Thessaloniki

UTH: University of Thessaly

BPI: Benaki Phytopathological Institute

3. EXECUTIVE SUMMARY

Project started in June 2014 and at present the following Actions are in progress:

Action A1: Productions of traps and attractants

Action A2: Preparatory monitoring of the demonstration areas

Action C1: Monitoring of projects environmental impact

Action C2: Monitoring of projects social-economical impact

Action D1: Dissemination

Action E1: Project management by SSIA

Action E2: Project Monitoring

The project began according to the timetable on 1st June 2014 and Actions A1, A2,

C1, C2, D1, E1 and E2 are in progress. The coordinating beneficiary performed all

necessary management activities so that the four scientific teams (SSIA, AUTH, UTH

and BPI) cooperate and function in synchronization.

As far as the technical part is concerned, SSIA, AUTH, UTH and BPI visited the

island of Chios „Kampos‟, selected the fields that will be monitored during Action A2

and will be participate in the demonstration Actions B1 and B2. A permit was

obtained for the implementation of the demonstration actions B1 and B2 from the

Hellenic ministry of Rural Development and Food for three years with the option to

revise the permit for another three years. The soil sampling and soil/substrate

evaluation by the SSIA produced a profile of the soils in the experimental orchards as

is described below in details in the section of the actions - output tables and

conclusions.

The efficacy test was decided to take place in the arboretum or in the nearby place of

the two associated beneficiaries AUTH, UTH by the responsible beneficiaries SSIA,

AUTH, UTH instead in the „Kampos‟ of the inland of Chios because was thought

would be of a better control and monitoring of the efficacy test. Apart from that there

was an extra opportunity the efficacy test to be tested at two different environmental

regions (Thessaloniki AUTH and Volos UTH) so the results would be representative

of the central and north regions of Greece. From the results obtained from the above

efficacy tests from the two places of the associated beneficiaries AUTH, UTH was

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found that the smallest amount of the BIODELEAR attractant was the one with the

best performance of attraction in both cases.

In the experimental fields in the „Kampos‟ of the inland of Chios before the

implementation of the Action B1, the following results were obtained: the med fly

population in the area of the inland of Chios was quite high even during late autumn

and winter; the percentage of fruit infestation rates ranged from 25 to 12% for

Clementine and the local Chios Mandarins respectively; the determination of the

environmental status such as the Biological diversity is in progress because due the

general practices (starting from the end June of 2015) is the most appropriate period

for measuring the biological diversity in the experimental fields.

The BPI team (leaders Dr. E. Bempelou, Dr. C. Anagnostopoulos and Ms P. Malatou)

undertook the fruit sampling according to the protocol described in the proposal of the

project. To this goal the associated beneficiary Benaki Phytopathological Institute

(BPI) during the action A2 undertook the task to (specifically during the time period

from August 2014 to February 2015) develop and validate multi residue analytical

methods for the determination of the residues of 334 plant protection products and 19

plant growth regulators according to the requirements of the guidance document

SANCO/12571/2013. So far the analyses are continued and are expected to be

completed at the end of August of 2015.

The WEB site was created and is available at www.biodelear.gr . Its further

development and support is ongoing.

Dissemination activities were also performed during the past 10 months, which

include creation of project logo, design of the dissemination material, project

presentation through the internet, as well as through meetings with local/regional

authorities and farmers associations.

4. ADMINISTRATIVE PART

4.1. Description of project management

Project Management (Action E1) and Project Monitoring (Action E2) began on 1st

June 2014 and will last for the entire project life. The coordinator, the researchers and

the staff of SSIA participate in this Action actively in order to achieve smooth

administrative and financial management of the project.

The fist kick off meeting of the Steering Committee (SC) was organized on 15 July

2014 in Athens at SSIA‟s premises, and each one of the beneficiaries presented their

Actions in order to find ways to increase the efficiency and the effectiveness through

cooperation. All the participants are seen in the following Table 1. Annex 1 includes

photos and the agenda of the kick-off meeting. Participants discussed several issues,

asked questions and received well-documented and clear answers. The project

monitoring structure was discussed and decided upon (details are seen in the

monitoring structure of the diagram of Action E2, Figure 1.)

http://www.biodelear.gr/

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Figure1.

Steering Committee (SC)

with senior representative from each partner

Chaired by Coordinator:

Dr Vassilis G. Mavraganis (SSIA)

Technical-Monitoring Committee (T-MC)

with one representative from each partner Chaired

by: Professor Nikos Papadopoulos (UTH)

Dissemination Committee (DC)

with one representative from each partner

Chaired by: Dr Sideris Theocharopoulos (SSIA)

Actions

B2

Leader

UTH

Actions

A1; A2; C1;

E1; E2

Leader

SSIA

Actions

B1

Leader

AUTH

Actions

None

Leader

BPI

Actions

D1

Leader

SSIA

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Table 1. 1st Kick off meeting participants

Name Institution Name Institution

Dr V.G. Mavraganis SSIA Dr C. Anagnostopoulos BPI

Dr V. Kavvadias SSIA Mrs P. Malatou BPI

Dr S. Theocharopoulos SSIA Prof. N. Papadopoulos-

through Skype

UTH

Dr E. Bempelou BPI Prof. N. Koulousis-

through Skype

AUTH

Dr K. Liapis BPI

The representatives from the associated beneficiaries UTH and AUTH (Prof. N.

Papadopoulos and Prof. N. Koulousis) were not able to participate personally in the

kick off meeting but only through Skype due to cancelations of flights (bad weather)

nevertheless the kick off meeting achieved the following goals.

Planning the assignment of the demonstration citrus orchards fields where the

project would take place

Planning the travelling to the inland of Chios „Kampos‟

A.1 Production of traps and attractants (April 2014 – March 2017)

A.2 Preparatory monitoring of the demonstrated areas (April 2014 –

September 2017)

The beneficiaries (Coordinating & Associated) to keep informing the

Timesheets forms according to the instructions of the LIFE web site.

The coordinator Dr Vassilis G. Mavraganis (SSIA), the administrative director Dr

Sideris Theocharopoulos (SSIA) and the representative of the administrative director

associate beneficiary BPI Dr E. Bempelou participated in the Kick off meeting of the

Life+ projects 2013 on 14 October 2014, in Kifisia, where were we presented our

project and were informed about the procedures and the requirements of the Life+

projects 2013 and also met Dr Anastasia Koutsolioutsou, the External Monitor

responsible for our project, from the LIFE monitoring company NEEMO EEIG –

Prospect C & S.

The 1st Steering Committee (SC) took place on 29

th and 30

th of September 2014,

Hotel Chandris in the island of Chios. The purpose of the Steering Committee (SC)

meeting in Chios was to select the citrus orchards by visiting the sites of the citrus

orchards in “Kampos” for the implementation of the project and to reach an

agreement with citrus orchards producers and discus details for the implementation of

the project between the beneficiaries (Associated and Coordinating) and the citrus

producers. Decisions were obtained to use apart from the three citrus orchards

according to actions B1 and B2 to include additional unmanaged orchards which have

been identified and will be used as a true control in our field trials (In these orchards

citrus producers are using the conventional way of protection from the med fly

insects) in order to obtain experimental and analytical power, of course the cost of

renting would not be affected since not any kind of protection would be applied by the

beneficiaries to the above additional unmanaged orchards. The participants of the

Steering Committee (SC) meeting are listed in Table 2. Photos of the Steering

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Committee meeting (SC) and the citrus orchards where the implementation will take

place Annex 2.

Table 2. 1st Steering Committee (SC) Meeting


Dr V.G. Mavraganis SSIA Prof. N.

Papadopoulos

UTH

Dr S. Theocharopoulos SSIA Prof. N. Koulousis AUTH

Dr E. Bempelou SSIA Mr. K. Tsartsalis Representative of

the Citrus

Producers in the

inland of Chios

Dr C. Anagnostopoulos BPI

The 1

st Technical –Monitoring Committee (T-MC) took place on the 1-3 December of

2014 between all the beneficiaries SSIA, AUTH, UTH and BPI in „Kampos‟ on the

island of Chios. The subject of the meeting was the collection of fruits for measuring

pesticides residues, soil samples for analysis, and the assessment of the percentage of

fruit infestation and med fly population. Apart from that the Team visited the Mayor

of Chios and the Vice Prefect to inform them regarding the BIODELEAR project

(photos in Annex 3) Table 3.

Table 3. 1st Technical- Monitoring Committee (T-MC) Meeting


Dr V.G. Mavraganis SSIA Ms P. Malatou BPI

Dr S. Theocharopoulos SSIA Prof. N. Papadopoulos UTH

Dr V. Kavadias SSIA Prof. N. Koulousis AUTH

Dr E. Bempelou BPI

Dr C. Anagnostopoulos BPI

The 2

nd T-MC Took place in the BPI with the SSIA, AUTH, & UTH on the 3

rd of

February to discuss the results so far obtained and was decided the following: 1st to

extend the A2 action for the sampling analysis of the insecticide residues in fruits

until the end of August for the B1 action because due to the preliminary results where

were found no insecticide residues and because there is a need to elucidate the real

chemical – profile of the pilot area an additional sampling of citrus fruits has to take

place during summer (June – August) and 2nd

to use extra true control experimental

orchards (with the conventional protection from the citrus producers) only the

monitoring part would be applied by the beneficiaries (Coordinating and Associated

beneficiaries) parallel to our implementation for the protection from the med fly in the

Actions A2, B1 and B2 Table 4.

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Table 4. 2nd

Technical- Monitoring Committee (T-MC) Meeting

Name Institution

1 Dr V.G. Mavraganis SSIA

2 Dr E. Bempelou BPI

3 Ms P. Malatou BPI

4 Dr. K. Liapis BPI

5 Dr C. Anagnostopoulos BPI

6 Dr V. Kavadias SSIA

7 Prof. N. Papadopoulos UTH

8 Prof. N. Koulousis AUTH

Dr V.G. Mavraganis the Leader of the Coordinating beneficiary could not attend to

the above schedule meeting due to health problems but was informed of the

whereabouts about the decisions of the Technical- Monitoring Committee (T-MC)

Meeting after his recuperation and agreed with the decisions taken by the Committee.

4.2. Organigramme of the project teams and the project management

structure

The management structure of BIODELEAR is organised into three layers. As seen in

the following scheme Figure 2, the responsible Directors, Group leaders and Group

members were defined by all beneficiaries. A list of all personnel working in the

project with their qualifications and their role in the project is included in Annex 4.

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Figure 2.

BIODELEAR MANAGEMENT STRUCTURE

Coordinating Institution: SSIA-ELGO DEMETER

Coordinator: Dr V.G. Mavraganis

Administrative Director

Dr Sideris Theocharopoulos

Financial Director

Ms Euaggelia Bania

SSIA

Group Leader

Dr V.G. Mavraganis

Team Members

Dr S. Theocharopoulos

Dr V. Kavvadias

Mr. K. Dimopoulos

Ms P. Kefalogiani

Ms N. Liakopoulou

Ms C. Liakopoulou

Mr. D. Arapakis

Mr. P. Lolos

Ms. E. Bania

Ms K. Petropoulou

Ms A. Katsoula

Mr. P. Tountas

Mr. T. Tsoutsikos

BPI

Group Leader

Dr E. Bempelou

Team Members

Dr K. Liapis

Dr G. Balayiannis

Dr C. Anagnostopoulos

Ms C. Malatou

AUTH

Group Leader

Assoc. Prof. N. Koulousis

Team Members

Prof. D. Koveos

Assis. Prof. A. Lagopodi

Prof. O. Spiroudi-Menkisoglou

Assis. Prof. G. Karaoglanidis

Lect. B. Maliogka

UTH

Group Leader

Prof. N. Papadopoulos

Team Members

Assis. Prof. C. Nakas

Assoc. Prof. C. Athanasiou

Assis. E. Vellios

Mr. C. D. Gerofotis

Prof. N. Danalatos

Prof. E. Khah

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Some changes made by SSIA concerning some more new members compare to when

the project was submitted in 2013 by the SSIA the salaries of the permanent personnel

was at a higher level but the government later in the year introduce substantial cuts on

the salaries of the permanent personnel (15% to 30% depending on the particular

case) as a result the daily rates were reduced substantially in addition was that some

of them were removed due to retirement or were departed from the SSIA to another

employment and apart from that in the foreseen Actions A2, B1 and B2 the amount of

work is also expected to increase as far concerns the amount of soil sampling.

Therefore from the above reasons and in order the project to keep up with the amount

of personnel costs for SSIA the person days have to be increased by 50%. The new

members were: Mr. K. Dimopoulos (Geologist, M.sc.), Ms C. Liakopoulou

(Agriculturist, B.sc), Mr. D. Arapakis (Agriculturist, B.sc), Mr. P. Lolos

(Agriculturist, B.sc), Ms K. Petropoulou (Technician) and Ms A. Katsoula

(Technician) due to their experience in soil sampling and soil/substrate evaluation.

Similar increase in permanent personnel was necessary for the other associated

beneficiaries (AUTH, UTH and BPI) but as far as concern the increase in person dates

didn‟t exceed the 10% limit.

5. TECHNICAL PART

The project‟s overall objective is the development of a sound, integrated and environmentally sustainable technology to address the Med flies in the Mediterranean region, using as an example the cultivation of citrus, implementation in real scale of an innovative, patented, environment friendly and low cost food attractant (BIODELEAR) for the control of the Med fly. The attractant is non-toxic to humans, attracts selectively females med fly and virtually none of the non-targeted insects. The target is the elimination of the insecticides use which are toxic to humans and to the environment. To achieve the project‟s objectives, the following actions and means are foreseen: 1. Synthesis of (50Kg according with the results from the efficacy test) of the innovative attractant Biodelear-synthesis 2. Efficacy lab tests for optimum quantity of Biodelear to be used in citrus orchards. 3. Demonstration activities in three citrus fields (i.e. two pilot scales and one real scale) 4. Determination of the fruiting % of the tested citrus orchards before and after the implementation of Biodelear. 5. Determination of the med fly population before/after implementation of Biodelear protection in the three pilot citrus orchards. 6. Development of a web-inventory 7. Risk assessment of the pilot areas at the beginning and by the completion of the project 8. Soil monitoring at the three pilot fields before and after the implementation of the mass trapping technique, (at least 20 soil parameters of each soil sample) 9. Confirmatory tests to verify the absence of pesticides in the pilot fields

10. Assessment of the effect of the conventional practice in comparison with the mass

trapping technique for the control of the Med fly insects (with a development of the

Integrated Strategy (IS)) on soil biodiversity

11. Development of an Integrated Strategy (IS) of the mass trapping technique for the

control of the Med fly insects

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12. Implementation of the developed Integrated Strategy (IS) at large scale (10 ha) for

its confirmation or if needed adjustment according to the pilot implementation results.

13. Scientific, technical and financial analysis of the obtained results against

anticipated environmental benefits. Strengths, Weaknesses, Opportunities and Threats

(SWOT) and Cost Benefits Analysis

14. Development of a functional and attractive web-site and an establishment of a

network.

5.1. Actions

5.1.1. Action A1: Production of traps and attractants

Foreseen start date: 01/06/2014; Actual start date: 01/06/2014; Foreseen end date:

31/01/2017

Status: in progress

Objectives: The action aims to (a) the synthesis of the attractants (b) the purchasing

the traps

The action began on 1st June, 2014 and anticipated that will be completed according

to the timetable. In this action the following actions were achieved from 1-06-2014

until 28 February of 2015: 1st

Synthesis of 30 Kg of the attractant Biodelear 5 Kg of

them were used for the efficacy test; 2nd

The purchase of the 100 IPM traps

(International Pheromone Monitoring traps) 40 traps of the 100 IPM traps were used

for the efficacy (10 IPM traps as blank, 10 IPM traps for the 17 gr, 10 traps for the 51

or 54 gr and 10 IPM traps for the 85 gr) the rest with 60 IPM traps for the med fly

monitoring with the commercial attractant of Biolure. 3rd

The Efficacy testing of the

innovative attractant Biodelear was measured in two different climatic environments

the one was from the Aristotelian University of Thessaloniki (AUTH) and the other

from the University of Thessaly (UTH).

Three quantities of Biodelear have been examined for best performance, towards

standardizing and providing input in actions B1 and B2.

Methodology

The efficacy test from the University of Thessaly (UTH).

During autumn 2014 we performed a field experiment aiming to compare the efficacy

of different doses of Biodelear to attract and capture Mediterranean fruit flies

(medflies). The experiment was established on the 18th

of September and traps were

checked until April 2015. The experiment was conducted in the area of Lechonia

(Magnesia) Greece, which is a coastal area where most of the fruits are cultivated in

mixed orchards of citrus, pome and stone fruit trees (mandarins, sweet oranges, apples

and peaches). The orchard consisted of 0.8 hectares of approximately 150 trees.

Approximately half of them were mandarins (Citrus nobilis) and half sweet oranges

(Citrus sinensis). The orchard includes also few scattered lemon trees (Citrus limon).

Trees were 10-14 year old, with their canopy formed in a free cup bush style. The

height of the canopy ranged from 1.8 to 4 meters.

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We followed a complete randomized block experimental design with six replicates

(blocks). All three doses of Biodelear were placed in McPhail type traps that were

filled with 250ml water and propylene glycol to capture the attracted insects. We used

three doses of the BIODELEAR (17, 51or54, and 85gr) impregnated in custom-made

sponge dispensers (vetex). Traps were checked at weekly intervals and all captured

insects were counted and removed, while dispensers were renewed every month.

Within each block, traps were placed 15 to 20 m apart from each other. Following

each trap service the position of the traps of each block was rotated. Each trap was

placed on the southwest part of the tree canopy at 1.8m from the ground, in a shaded

part of the tree canopy. In some cases appropriate pruning was performed to increase

trap exposure. Blocks were located at distance longer that 30m among each other.

Statistical analysis was performed using one-way analysis of variance (ANOVA) of

the total catches per trap using the statistical package SPSS 20. Data were transformed

to sqrt(x) to normalize distribution and achieve equal variances when needed. Means

were separated using the criterion Tukey‟s HSD (a = 0.05). A repeated measures

analysis of variance using the weekly captures as the repeated factor was also

performed considering captures up to the end of December. No flies were captured

after this period. Final Results and Conclusion Annex 7.

The efficacy test from the Aristotelian University of Thessaloniki (AUTH).

The study was conducted on the Aristotle University of Thessaloniki farm (40.3°

northern latitude; sea level) in northern Greece. Ten out of 200 ha total surface area of

the farm were occupied by pome fruits, stone fruits, and a few other hosts. In

particular, apples (Malus sylvestris Mill.) occupied 5 ha; pears (Pyrus communis L.)

occupied 0.7 ha; apricots (Prunus armeniaca L.), peaches [P. persica (L.) Batsch],

cherries (P. avium L.), sour cherries (P. cerasus L.), and plums [P. domestica (L.)]

occupied a total of =0.9 ha; walnuts (Juglans regia L.) occupied 0.4 ha; kiwis

(Actinidia chinensis Planch.) occupied 0.9 ha; and olives (Olea europaea L.)

occupied 0.6 ha. The test was performed on apples trees. In that area apple trees

mature their fruits in September and October.

The three different quantities of Biodelear tested were 17, 51 and 85 gr impregnated

in custom-made sponge dispensers (vetex). We followed a complete randomized

block experimental design with six replicates (blocks). We used plastic Mc Phail-type

traps with 250 ml of a 10% water solution of propylene glycol added to the lower part

of the trap. One set of three traps was suspended on each of six rows of apple trees in

random order at a height of 1.5–2 m above ground. Within each block, traps were

placed 15 to 20 m apart from each other. Following each trap service the position of

the traps of each block was rotated. Each trap was placed on the southwest part of the

tree canopy at 1.8m from the ground, in a shaded part of the tree canopy. In some

cases appropriate pruning was performed to increase trap exposure. Blocks were

located at distance longer that 30 m among each other. Traps were rotated within each

row. The traps were deployed on 22 September 2014. The traps were checked every

week and all the flies were counted, sexed, identified and removed. Traps checks

continued until the end of April.

Statistical analysis was performed using one-way analysis of variance (ANOVA) of

the total catches per trap using the statistical package SPSS 20. Data were transformed

to log(x + 1) to normalize distribution and achieve equal variances when needed.

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Means were separated using the criterion Tukey‟s HSD (a = 0.05). Final Results and

Conclusion Annex 7.

5.1.2. Action A2: Preparatory monitoring of the demonstration areas

Foreseen start date: 01/06/2014; Actual start date: 01/06/2014; Foreseen end date:

30/09/217

Status: in progress

Objectives: The action aims to (a) recording and evaluating of the environmental

status of the pilot areas and the demonstration area (b) measuring the fruit infestation

percentage (c) measuring the med fly population (d) evaluating the impacts of these

practices on farm environment (soil quality, biological diversity, pesticides use and

management) (e) evaluating the production (generated cost, amount and quality of

fruits) (f) the efficacy test. The above evaluations would be from the conventional

practices of protection.

The action began on 1st of June, 2014 and is anticipated that will be completed

according to the timetable (i.e. on 30/9/2017) nevertheless the total duration of the

action A2 would be about one year although it will take a span of three years time.

During the last seven months (September of 2014 to January 2015) the two citrus

orchards of 1 ha each (one for pilot tests and the other as control field in which

conventional protective practices are followed) were selected with the task to measure

and evaluating the environmental status of the pilot areas before the implementation

of the protection from the med flies.

The SSIA has obtained licences from the Ministry of Agricultural and Production of

Food for three years for the use of the two 1 ha citrus orchards and the 10 ha citrus

orchards with the capacity to revised for another three years.

The SSIA with the team leaders Dr. V.G. Mavraganis, Dr. S. Theocharopoulos and

Dr. V. Kavvadias undertook the samplings for the monitoring of soil quality, the

identification of soil indicators and the evaluation of soil quality in relation to

cultivation practices. The team visited the island of Chios „Kambos‟ area in 29-30

September, 2014 and 1-3 December, 2014. 52 soil samples were collected in total

from citrus orchards in the Chios „Kambos‟ area of the whole area depicted in the

photograph annex 2. The citrus orchards where the BIODELEAR project would take

place:

1) Latitude 38° 19´ 47.34´´ N; Longitude 26° 7´ 58.82´´ E (Zanis Citrus Orchard)

2) Latitude 38° 19´ 10.82´´ N; Longitude 26° 7´ 46.10´´ E (Perleas 1 Citrus

Orchard)

3) Latitude 38° 19´ 17.87´´ N; Longitude 26° 7´ 24.20´´ E (Gianniris Citrus

Orchard)

4) Latitude 38° 19´ 20.33´´ N; Longitude 26° 7´ 36.35´´ E (Perleas 2 Citrus

Orchard)

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(Are included the pilot and the control areas) from different sites and depths (the

surface range from 0-30 cm and from 30 cm to 60 cm) and were transferred to the lab

for analysis. For the time being most of the analyses (23 soil parameters) were

completed. All measured soil parameters, analysis, results and conclusions are

included in Annex 5.

The BPI team (leaders Dr. E. Bempelou, Dr. C. Anagnostopoulos and Ms P. Malatou

and Dr. George Balagiannis) undertook the fruit samplings according to the protocol

described in the proposal of the project. The number of samples is higher than the

proposed (133 instead of 120 as described in Action A2). The number of 120 fruit

samples mentioned in the proposal was based on the theoretical plantation of a 1 ha

orchard. Since the experimental orchards were more extensively planted than

expected, they were divided to the appropriate number of plots in order to have a

representative sampling (see the area where would take place the sampling are

included the pilot and control 1ha areas annex 2). The final number of fruit samples

was amended respectively to the number of plots.

During action A2 the associated beneficiary Benaki Phytopathological Institute (BPI)

and in specific for the time period from August 2014 to December 2014 developed

and validated multi residue analytical methods for the determination of the residues of

334 plant protection products and 19 plant growth regulators according to the

requirements of the guidance document SANCO/12571/2013. The methods were

based on the QuEChERS method (EN 15662) and the use of sensitive

chromatographic techniques (GC/ECD/NPD, GC/MS, GC/MS/MS and LC-MS/MS).) The results, analysis and conclusions see Annex 6

The Associated beneficiaries UTH and AUTH

Selection and renting of three orchards in Chios

Following several field visits in the area of Kampos Chios, during winter and spring

2014-2015 together with the group of the University of Thessaly & of the Aristotelian

University of Thessaloniki and considering also the needs of the other beneficiaries

we selected the experimental farms that are currently used for medfly population

monitoring, management and biodiversity estimates. Instead of using three orchards

as it is stated in the proposal following a management committee meeting we decided

to include an additional orchard in order to obtain experimental and analytical power.

Additional unmanaged orchards have been identified and will be used as a true

control in our field trials.

Biological diversity determination using pit fall traps and knock-out insecticide

methods

Following the rending of farms that was concluded few weeks ago, we have been

working on the methodology regarding assessment of insect biodiversity in the

experimental farms and in general in the area of Chios. The respective experiments

and the deployment of specific (pitfall type traps) will start at the end of June 2015. A

monthly recording of the insect fauna in the orchard is scheduled. We are currently

working on the methodology to assess the above ground insect biodiversity and we

are considering two alternative methods, either appropriate traps or knockdown

following a pyrethroid spraying.

15


Our planning for the ground biodiversity includes the deployment of 5-6 pitfall traps

in each experimental plot. Pitfall traps will be filled with an appropriate amount of an

aqueous solution of propylenoglygol (5%). Traps will be exposed in the field for 24 or

48 hours. All captured insects will be transferred to our laboratory in Volos where

they will be identified using appropriate dichotomous keys.

Monitoring of three fields for measurement of infestation percentage and

determination of the number of med fly population present

Fruit sampling (mandarin and clementine fruits) was conducted in the beginning of

December 2014. Fruits were transferred to the laboratory of Entomology and

Agricultural Zoology at the University of Thessaly, Greece and the proportion of

infestation was determined. We collected ripe and ripening fruits from the trees or

recently fallen to the ground of clementine and local mandarins. Two hundred fruit

from each variety were randomly selected from three different orchards. Upon arrival

in the laboratory fruits were placed in plastic containers over a layer of dry sand.

Fruits were maintained at constant laboratory conditions (25°C, 50%RH and 14:10

L:D) regularly inspected and those showing typical symptoms of medfly infestation

were recorded. The sand of the container was also sieved and all pupae were collected

and counted.

To identify the level of the insect population we deployed 9 plastic McPhail-type

traps in the experimental orchards baited with Βiolure dispensers. To capture the

attracted flies, 250 ml of a 10% water solution of propylene glycol were added to the

lower part of the IPMT trap. The traps were deployed on 30 October 2014. Trap

check and servicing was performed weekly. During the winter months trap checks

were made every two weeks. The number of male and female medflies as well as and

non-target insects captured were scored. Preliminary Results and Conclusions Annex

8.

5.1.3 Action B1: Implementation of innovative Med traps at pilot scale

Foreseen start date: 01/06/2015; Foreseen end date: 31/12/2016

(1/6/2015-31/12/2016)- Not foreseen for this period

Objectives: The action aims to developed of an Integrated Strategy (IS) through a two

years implementation of the Biodelear attractant using two citrus orchards one the

pilot 1 ha and the other the control 1 ha. In addition to the above the following

evaluations also would take place (a) recording and evaluating of the environmental

status of the pilot area and the control area during the implementation of protection

from the med fly (b) measuring the fruit infestation percentage at the end of the

implementation of protection from the med fly (c) measuring the med fly population

(d) evaluating the impacts of that of these practices on farm environment (soil quality,

biological diversity, pesticides use and management) (e) evaluating the production

(generated cost, amount and quality of fruits).The developed IS will be confirmed and

/ or adjusted during the following real application of Action B2.

5.1.4. Action B2: Implementation of innovative Med traps at real scale

16



Status: Not foreseen for this period

Objectives: The action will last two years and its main objective is the implementation

of the Integrated Strategy (IS) developed during Action B1by using as attractant the

Biodelear, its confirmation or adjustment if needed.

In a similar way as in the Action B1the following evaluations would take place (a)

recording and evaluating of the environmental status of the demonstration area

during the implementation of protection from the med fly (b) measuring the fruit

infestation percentage at the end of the implementation of protection from the med fly

(c) measuring the med fly population (d) evaluating the impacts of that of these

practices on farm environment (soil quality, biological diversity, pesticides use and

management) (e) evaluating the production (generated cost, amount and quality of

fruits). The IS will be extended by considering many variables to cover the

Mediterranean area and thus an Integrated Mediterranean Strategy (IMS) will be

developed.

5.1.5. Action C1. Monitoring of project’s environmental impact


Status: In Progress

Objectives: Action C1 will last 60 months and aims to the following 1st monitor the

effects of project’s actions on the state of the environment on a regular basis; and 2nd

asses the project contribution to the development sustainable procedure of crop

protection without the use of pesticides and the subsequent production of

environmentally safe products.

In the framework of Action C1, allot of data have been collected for the

environmental status prior to the implementation of the protection of the med fly with

the Biodelear attractant on the citrus orchards area as are described in the action A2.

After the completion of the collected data the monitoring of project‟s environmental

impact will be applied on regular basis especially on the Actions B1 and B2.

5.1.6. Action C2: Monitoring of project’s social-economical impact


Status: In Progress

Objectives: Action C2 will last 60 months and aims to derive specific indicators to

monitor and to measure social-economic impacts. The responsible beneficiary will

perform surveys, concentrating on main target groups, which are anticipated to assist

in timely recording of indicators for monitoring the strategy impact.

The SSIA is the beneficiary who is responsible for the implementation of this action,

in cooperation with BPI, AUTH and UTH. For the purpose of this Action, two visits

(29-30 September of 2014 and 1-3 December of 2015) of the beneficiaries took place

17


in the island of Chios informing the local citrus producers and the local authorities

about the aim of the project and getting information of the local habits and existing

methodologies and databases for identifying specific indicators that will act as the

baseline for the initial selection of social-economic indicators that will be undertaken

during the duration of the project. Near the end of the project would be the beginning

of a development of a preliminary questionnaire concerning social-economic impacts

of the project.

5.1.7. Action D1: Dissemination


Status: In Progress

Objectives: Action D1will last to the entire duration of the project and on this action

the following would be developed. 1st The development of a functional and attractive

project web site. 2nd

Establishment of a net work between research centres,

universities, farmers and their associations, regional and national authorities,

representative of industry/market community, scientists from other already existing,

European networks and also with other relative LIFE projects relative to the pest and

agricultural production management. 3rd

Dissemination of project’s achievements to

stakeholders, scientists, policy makers and mainly to producers in Greece and in

other Mediterranean countries.

On this action during the last seven months were created the following:

The web site www.biodelear.gr; the logo of the project was created and used in the

web site and in all dissemination activities as well as the logo of the EU and the

sentence like “with the contribution of the LIFE+ financial instrument of the

European Union”. The web site and any other actions regarding the dissemination

action of the project is in accord with the Common Provisions

Biodelear beneficiaries also contacted other LIFE projects and informed the

involvedscientists regarding the project. Specifically, discussions were made with

Agrostat (LIFE11 ENV/GR/951), OliveClima (LIFE11/ENV/GR/942), LIFE

CONOPS .

5.1.8. Action D2: After LIFE Communication Plan


Status: Not foreseen for this period

Objectives: Action D2 aims to develop a strategic that will ensure the dissemination

and communication of the project results after its completion.

5.1.9. Action E1: Project management by SSIA


Status: In Progress.

http://www.biodelear.gr/

18


Objectives: To ensure timely implementation of the project, good cooperation among

Beneficiaries, quality assurance of the deliverables and day to day control of the

administrative and financial issues of the project.

Reports and Status:

- Inception report (submitted on ...2015)

- Progress report (until 1 December 2015)

- Midterm report (until 1 December 2016)

- Progress report (until 1 December 2017)

- Final report (until 1 May 2019)

The project management was defined and is functioning well. The coordinator and the

management group of SSIA are in touch very often and meetings are held at least

once a month. All important decisions about the project are discussed in this group.

Agreement for procedures for project implementation, communication and

cooperation were agreed on in the beginning of the project period during the kick off

meeting and also via e-mails between the beneficiaries. The project group functions as

a dialogue group where progress and other relative issues are discussed.

5.1.10. Action E2: Project Monitoring


Status: In Progress

Objectives: Measure and document the effectiveness of the project actions as

compared to initial situation, objectives, expected results and environmental impacts.

As mentioned, the project has a structured monitoring system, which ensures the

control of the progress in relation to the initial aims and targets. The members of the

Technical-Monitoring Committee (T-MC) and of the Dissemination Committee were

defined during the kick off meeting. Output indicators were discussed between the

members of the Committee and defined (see Annexes 5, 6, 7 and 8). The first meeting

of the T-MC took place on 15 July in Lykovrisi, Athens (SSIA) with the participation

of BPI, AUTH, UTH and SSIA. The subject of the meeting was first the planning of

the assignment of the demonstration citrus orchards fields where the project would

take place, second the planning of the travelling to the inland of Chios „Kampos‟, a

set of timetables for each one of the beneficiaries to keep them aware of their duties

and the timesheets form for involved scientists and institutions‟ staff according to the

instructions of the LIFE web site.

5.2 Availability of appropriate licences and authorisations

One appropriate licence was obtained from the Agricultural Ministry of Food and

Production for the use of the 12 ha citrus orchards in the „Kampos‟ in the inland of

Chios for three years with the option to revised the licence with another application

after expire of the permit (Annex 9).

19


5.3 Envisaged progress until next report.

Action A1: The action will be continued to be in progress and is anticipated that will

be completed according to the timetable (i.e. 31/01/2017).

Action A2: SSIA will continue with new soil samplings in the pilot area within the

timeframe of the action and analyses on June and August of 2015. The BPI will

continue to analyse and do new fruit samplings on June and August of 2015. The

AUTH and UTH will do measure the Biological-diversity of the ecosystem during the

period of the summer starting at the end of June the year 2015 and preparations for the

Action B1 starting from June of 2015.

Action B1: On June 2015 will start the action B1 with the first fruiting period to end

at the end of the year 2015 and at the beginning of the year 2016.

Action B2: The action will be fully develop the year 2017

Action C1: Action C1 will continue its implementation according to schedule.

Action C2: Specific indicators that will act as the baseline for the initial selection of

social-economic indicators will be developed.

Action D1: The project teams will focus on the development of the project network

and is anticipated that until the next report, most of the targeted stakeholders would

have been informed and invite to participate. The beneficiaries will also focus on

general dissemination activities in order to promote the project and its anticipated

benefits. The project is planned to participate in a meeting in the inland of Chios to

inform the local authorities and the citrus producers about the targets of the project in

the coming month of June of 2015. Three metallic boards would be created at the site

of the pilot control and demonstration areas of the citrus orchards.

Action E1: Management structure functions in a satisfactory way, SSIA will maintain

it, while corrective actions will be performed, if needed.

Action E2: SSIA will continue to monitor the project progress relative to its

objectives. Meetings of the project committees will be organized if needed. The

steering Committee will meet before the beginning of the actions B1 and B2 to

evaluate and maximize the effectiveness of the activities.

No changes in the planned actions as defined in the proposal are foreseen until now.

20


Tasks/

Activities

20105

2016

2017

2018

2019

2T 3T 4 T 1T 2T 2T 4T 1T 2T 3T 4T 1T 2T 3T 4T 1T 2T 3T 4T 1T 2T

Overall project schedule

Proposed

O

O

X

O

X

Actual

Action A1. Preparatory actions

Productions of traps and

attractants

Proposed

Actual

Action A2. Implementation of

innovative Med traps at pilot scale

Proposed

Actual

Action B1. Implementation of

innovative Med traps at pilot scale

Proposed

Actual

Action B2. Implementation of

innovative Med traps at real scale

Proposed

Actual

Action C1. Monitoring of

project‟s enviromental impact Proposed

Actual

Action C2. Proposed

Actual

Action D1. Proposed

Actual

Action D2. Proposed

Actual

Action E1. Proposed

Actual

Action E2. Proposed

Start date Mid-Term End date

X=Progress reports

21


Actual

6. Financial part

6.1.Putting in place of the accounting system.

Most of the accounting system was to do with consumables and travel

expenses and personnel costs so far until the date of 28/2/2015

6.2.Continued availability of co financing.

There is a continued availability of co financing

6.3.Costs incurred (summary by cost category and relevant comments).

The costs incurred so far to date 28/2/2015 from the start comes to

133553.27 €

Fill in the following table concerning the incurred project costs:

Budget breakdown categories Total cost in € Costs incurred from the start

date to 28.2.2015 in €

% of total costs

1. Personnel 1496173 86298.56 5.77

2. Travel and subsistence 146494 11935.04 8.14

3. External assistance 71000 0 0

4. Durable goods 0 0 0

Infrastructure 0 0 0

Equipment 0 0 0

Prototype 0 0 0

5. Land purchase / long-term lease 0 0 0

6. Consumables 323001 24039.91 7.44

7. Other Costs 24504 2639.67 10.77

8. Overheads 144282 8737.5 6.05

TOTAL 2205454 133553.27 6.05

*) So far not any revised budget modification exists.

**) The calculation of the percentages by budget lines: The 5.77 % of the

budgeted personnel costs from the start date to 28. 2.2015

22


So far there are no any particularly discrepancies, nevertheless in the coming year

there is a need to transfer some money from the consumables of the coordinating

beneficiary SSIA to the associated beneficiaries BPI and UTH personnel due to the

fact that the amount of work as far as concerns the fruit sampling for the associated

beneficiary (BPI) (Since the experimental orchards were more extensively planted

than expected, they were divided to the appropriate number of plots (increase in plots)

in order to have a representative sampling) will increase and similarly the amount of

work will increase for the associated beneficiary UTH because deployment of the

mass and medfly population monitoring grid will be more extensive and intensive

since additional orchards have been included. And biodiversity assessment will

operate in a broader area and higher intensity. As far as the cost of the consumables is

concerned the LIFE BIODELEAR project on the coordinating beneficiary SSIA

would not be affected, because we recently determined that preparation of the

BIODELEAR bait will cost substantially less that initially estimated. For any future

changes on the budget reallocations of course the European Commission will be

notified.

Please fill in also the following additional table (recommended). When compiling the

information please refer to Form B of the proposal:

Action number and name Foreseen costs

Spent so far Remaining Projected final cost

Action A1 Production of traps and attractants

147799 27233.64 120565.36 147799

Action A2 Preparatory monitoring of the demonstration areas

306935 87645.99 219289.01 306935

Action B1 Implementation of innovative Med traps at pilot scale

388034 4105 383929 388034

Action B2 Implementation of innovative Med traps at large scale

451634 97.46 451634 451634

Action C1 Monitoring of project’s environmental impact

77345 0 77345 77345

Action C2 Monitoring of project’s social-economical impact

43370 644.83 42725.17 43370

Action D1 Dissemination 48452 2184.45 46267.55 48452

Action D2 ‘After-Life + Communication plan’

0 0 0 0

Action E1 Project Management by SSIA

162816 1468.06 161347.94 162816

Action E2 Project Monitoring

137300 1436.34 135863.66 137300

TOTAL 1496173 124816.17 1371356.83 1496173

23


7. Annexes

Annex 1: Photos and the agenda of the 1st Kick-off meeting

Agenda

BIODELEAR LIFE13 ENV/GR/000414 Addressing Med fly with an innovative and environment friendly attractant

through an Integrated Pest Management Strategy

1st Kick off Meeting

15 JULY 2014, Lykovrisi, Athens

Program

10:30-11:00 Introductory Welcome to the Steering committee from the Director of the Soil Institute of Athens Dr Sideris Theocharopoulos

11:00-11:45 Actions A1, A2, B1 and B2 -General description of the project management, monitoring and progress of the project Dr V. G. Mavraganis

11:45-12:15 Break

12:15-13:00 Actions A1, A2, B1 and B2 (Progress, preparations of the preparatory experiments – choosing the land for future use - hiring land) Professor N. Papadopoulos

13:00-13:45 Actions A2, B1 and B2 (Description of the Actions) Measurements of the pesticides or pesticides residues) Dr E. Bebelou

13:45-14:30 Lunch

14:30-15.30 General Discussions on the project

24


25


26


Annex 2: The 1st Steering Committee (SC) Meeting

The 1st Steering Committee (SC) took place on 29

th and 30

th of September 2014,

Hotel Chandris in the inland of Chios. The Steering Committee (SC) meeting in

27


the Chios inland was to select the citrus orchards by visiting the sites of the citrus

orchards “campos” for the implementation project and to reach an agreement with

citrus orchards producers and discus details for the implementation of the project

between the beneficiaries.

28


The citrus orchards where the implementation of the project would take place

(The names of the citrus orchards A. Zanis; B. Zanis; B. Perleas; E. Gianniris

(Elaiotrivio))

The ‘Kampos’ of the Inland of Chios

The citrus orchards where the BIODELEAR project would take place:

1) Latitude 38° 19´ 47.34´´ N; Longitude 26° 7´ 58.82´´ E (ZANIS CITRUS

ORCHARD)

2) Latitude 38° 19´ 10.82´´ N; Longitude 26° 7´ 46.10´´ E (PERLEAS 1

CITRUS ORCHARD)

3) Latitude 38° 19´ 17.87´´ N; Longitude 26° 7´ 24.20´´ E (GIANNIRIS

CITRUS ORCHARD)

4) Latitude 38° 19´ 20.33´´ N; Longitude 26° 7´ 36.35´´ E (PERLEAS 2

CITRUS ORCHARD)

29


30


Annex 3: 1st Technical –Monitoring Committee (T-MC) Meeting

31


The 1st Technical –Monitoring Committee (T-MC) took place on the 1-3

December of 2014 between all the beneficiaries SSIA (Dr V.G. Mavraganis, S. Theocharopoulos and V. Kavvadias), AUTH (Prof. N. Koulousis), UTH (Prof. N.

Papadopoulos) and BPI (Dr E. Bempelou, Dr C. Anagnostopoulos and Mrs P.

Malatou) in the Chios inland of „Kampos‟. Collecting soil samples fruit samples

and evaluating the med fly population and med fly infestation.

32


The representative team of the project BIODELEAR [the beneficiaries SSIA (Dr

V.G. Mavraganis, and Dr S. Theocharopoulos), AUTH (Prof. N. Koulousis), UTH

(Prof. N. Papadopoulos) and BPI (Dr E. Bempelou, Dr C. Anagnostopoulos and

Mrs P. Malatou)] together with the representative of the citrus orchard producers

Mr Kosta Tsartsali visited and informed the Mayor of Chios Mr Manoli Vourna

on the 2 December of 2014 on the project BIODELEAR.

The representative team of the project BIODELEAR [the beneficiaries SSIA (Dr

V.G. Mavraganis, and Dr S. Theocharopoulos), AUTH (Prof. N. Koulousis), UTH

(Prof. N. Papadopoulos) and BPI (Dr E. Bempelou, Dr C. Anagnostopoulos and

Mrs P. Malatou)] together with the representative of the citrus orchard producers

Mr Kosta Tsartsali visited and informed the Vice Prefect of Chios Mr Stamatis

Karmantzis on the 2 December of 2014 on the project BIODELEAR.

Annex 4: List of all personnel working on the project with their qualifications

and their role in the project

List of all personnel working on the project with their qualifications and their role in the

project

Name/Institution Qualification Role in the project

Dr. Vassilis G.

Mavraganis/SSIA

Chemist A1: Synthesis of the attractant

Biodelear, Trap production.

A2: Participating in the

monitoring of the ecosystem in the

citrus orchards cultivation areas,

data evaluation of soil indicators,

soil analysis, in the efficacy test,

of the med fly population and med

fly infestation, pesticide residues

in the ecosystem of the three citrus

33


orchards (control, pilot and large

scale)

B1: Participating in the

development of the protection of

the med fly in the pilot citrus

orchard and control citrus orchard.

B2: Participating in the

development of the protection of

the med fly in the large scale citrus

orchard.

C1: Monitoring of project‟s

environmental impact

C2: Monitoring of project‟s social-

economical impact

D1: All dissemination activities

E1+E2: Project management and

monitoring

Dr Victor Kavvadias/SSIA Forester, Soil

Fertility

A2: Data collection, monitoring


soil analysis of the three citrus


scale)

B1: Data collection, monitoring


soil analysis on the pilot and

control citrus orchards

B2: : Data collection, monitoring


soil analysis on the large scale

citrus orchard




economical impact



monitoring

Dr Sideris

Theocharopoulos/SSIA

Pedologist A2: Data collection, monitoring


soil analysis of the three citrus


scale)

B1: Data collection, monitoring


soil analysis on the pilot and

control citrus orchards

B2: : Data collection, monitoring


soil analysis on the large scale

citrus orchard




economical impact

34




monitoring

Nektaria Liakopoulou,

M.sc./SSIA

Chemist

Instrumental

analysis

A2: Data collection, soil analysis

on the pilot and citrus orchards

B1: Data collection, soil analysis



on the large scale citrus orchard

Konst. Dimopoulos,

M.sc./SSIA

Geologist A2: : Data collection, soil analysis

on the pilot, control and large

scale citrus orchards B1: Data

collection, soil analysis on the

pilot and citrus orchards



Dimitris Arapakis/SSIA Agronomist A2: Data collection, soil analysis


scale citrus orchards





Polykarpos Lolos/SSIA Agriculturist A2: Data collection, soil analysis







Christina Liakopoulou/SSIA Agriculturist A2: Data collection, soil analysis







Pantelis Tountas/SSIA Technician

Agronomist








Pelagia Kefalogiani/SSIA Technician

Agronomist








Evangelia Bania/SSIA Financial staff E1: financial monitoring of the

project

Athanasios Tsoutsikos/SSIA Worker A2: Soil sampling, pre-treatment

35




B1: Soil sampling, pre-treatment


B2: Soil sampling, pre-treatment


Athanasia Katsoula Technician

Agronomist








Ntina Petropoulou Technician

Agronomist








Dr Eleutheria Bempelou/BPI Agriculturist

specialist in the

agricultural

pesticides

(BPI group leader in all Actions

BPI is involved) coordinates

scientific, technical, financial and

administrative work of BPI. C1:

Monitoring of project‟s




monitoring

Dr Konstantinos Liapis Chemist/ speciality

in the analysis of

agricultural

pesticides

A2: Data collection, pesticides

residues analysis on the pilot,

control and large scale citrus

orchards

B1: Data collection, pesticides

residues on the pilot and citrus

orchards


residues analysis on the large scale

citrus orchard





monitoring

Dr George Balayiannis Chemist/ speciality

in the analysis of

agricultural

pesticides




orchards



orchards



citrus orchard C1: Monitoring of

36


project‟s environmental impact



monitoring

Dr Christos Anagnostopoulos Agriculturist/

speciality in the

analysis of

agricultural

pesticides




orchards



orchards







monitoring

Ms Christina Malatou Agronomist/

speciality in the

analysis of

agricultural

pesticides




orchards



orchards







monitoring

Dr Nikos Papadopoulos Entomologist/expert

on diptera fruit flies

such us the med fly

Ceratitis capitata

(UTH group leader in all Actions

UTH is involved) coordinates


administrative work of UTH.

A2: Data collection, efficacy test,

med fly population and med fly

infestation, biological diversity on

the pilot, control and large scale

citrus orchards

B1: Data collection, efficacy test,


infestation biological diversity on

the pilot and citrus orchards




the large scale citrus




economical impact


37



monitoring

Assis. Prof Christos Nakas Statistics A2: Data collection, efficacy test,




citrus orchards









Dr Christos Athanasiou Entomologist A2: Data collection, efficacy test,




citrus orchards









Dr Evangelos Vellios Plant pathology A2: Data collection, efficacy test,




citrus orchards









Mr Christos D. Gerofotis Entomologist A2: Data collection, efficacy test,




citrus orchards









38


Prof Nikolaos Danalatos Plant Ecology A2: Data collection, efficacy test,




citrus orchards









Prof Ebrahim M Khah Plant breeding and

genetics





citrus orchards









Dr Nikos Koulousis Entomologist/expert

on diptera fruit flies

such us the med fly

Ceratitis capitata

(AUTH group leader in all Actions

AUTH is involved) coordinates


administrative work of AUTH.





citrus orchards












economical impact



monitoring

Prof Dimitris Koveos Entomologist A2: Data collection, efficacy test,




39


citrus orchards









Assis Prof A. Lagopodi Plant Protection A2: Data collection, efficacy test,




citrus orchards









Prof Ourania Spiroudi-

Menkisoglou

Plant Protection A2: Data collection, efficacy test,




citrus orchards









Assis. Prof. George

Karaoglanidis

Plant Protection A2: Data collection, efficacy test,




citrus orchards









Lect. Barbara Maliogka Plant Protection A2: Data collection, efficacy test,




citrus orchards

40










Annex 6 Preliminary Results and Conclusions from the analysis of the fruit

samplings. (Action A2) Associated beneficiary (BPI) responsible

The results of the validation of the analytical methods showed acceptable sensitivity,

by providing a limit of quantification in 0.01mg/kg based on the lowest validation

level. Moreover, the obtained recoveries were all within the acceptable range of 70-

110% for both methods, giving acceptable accuracy. The relative standard deviations

calculated were all lower than 20%, showing acceptable reproducibility. Therefore the

analytical methods are considered reliable and suitable for the analysis and the

determination of pesticide residues and plant growth regulators in accordance to the

requirements of European Commission.

Results and Conclusions of (BPI)

The analysis of the samples has now been completed. No detectable residues of plant

protection products or plant growth regulators were determined. This first sampling

can be considered as a preliminary monitoring (screening) in random citrus orchards

of the pilot area of Campos Chios, providing an estimation of the potential

contamination of the cultivated areas in the certain time period of sampling. The fact

that no detectable residues were observed is attributed to the previous application of

plant protection products according to the Good Agricultural Practice (GAP). In order

to elucidate the chemical -profile of the pilot area, an additional sampling of citrus

fruits is going to take place during summer (June to August 2015) when farmers apply

plant protection products for the control of Ceratitis Capitata. The sampling time will

be adjusted to the PreHarvest Intervals (PHIs) as reported in the registered GAPs of

the applied formulations.

41


Deliverable Products of the Project

Name of the Deliverable Number of the associated

action

Deadline

Purchase traps and attractants for

the monitoring of the med fly and

the efficacy test

A1 31/12/2014

Synthesis of the Biodelear

attractant 30 Kg

A1&A2 28/2/2015

Efficacy testing of the innovative

attractant Biodelear

A1 31/12/2014

Renting the two citrus orchard

areas of 1 ha

A2 31/12/2014

Obtaining The Permits from The

Ministry of Rural Development

and Food

A2 31/12/2014

Preliminary results of soil

sampling analysis, Preliminary

results of insecticide residues in

the citrus fruits, Preliminary

results of medfly population and

Preliminary results of fruit

infestation percentage

A2 28/2/2014

Web site of the project D1: Dissemination 31/12/2014

Documents

LIFE Project Number LIFE13 ENV GR/000414 Inception Report … · The 1st Steering Committee (SC) took place on 29th and 30th of September 2014, Hotel Chandris in the island of Chios