If you can't read please download the document
Upload
archibald-bruce
View
223
Download
0
Embed Size (px)
DESCRIPTION
Protocista Algae Plants
Citation preview
Life is a tiny electric current kept moving by the sun Svent
Gorki
Photosynthesis Life is a tiny electric current kept moving by the
sun Svent Gorki Protocista Algae Plants Photosynthesis
Equation
carbon dioxide + water glucose + oxygen 6CO2 + 6H20 C6H1206 White
Light Electro magnetic spectrum So Why Green? Chloroplasts
Fluorescence of extracted chlorophyll
Chlorophyll plays an important role in photosynthesis as it absorbs
the energy form the sun Fluorescence of extracted chlorophyll
Absorption and action spectrum of chlorophyll Light Independent
Stage 6 CO2 6H2O C6H12O6 24 H 6O2 Oxygen produced 12H2O SUN
Chlorophyll Water used Light dependent Measuring the rate of
photosynthesis Extraction of chlorophyll and absorption
spectrum
Assessment -Data Collection and Processing, Conclusion and
Evaluation, manipulative skills Extraction of chlorophyll Dip fresh
leaf material into boiling water first to kill the cells and to
make the cell membrane fully permeable to the solvent. Finely cut
about 10g of leaf material (excluding the midrib and petiole) into
a mortar and add a small amount of washed and dried sand. Grind up
the leaf matter with 20cm3 of propanone, using a pestle, to make a
concentrated leaf extract. Either filter the pigment solution or
decant it (using a glass rod to hold back the debris), and place in
a small glass centrifuge tube. Centrifuge the extract briefly, in
order to clear all suspended organic matter. Pour the supernatant
solution into a small glass tube, seal with a bung, label it, and
stand this chlorophyll extract in the dark, anice bath, until
required. Measuring absorption of light by chlorophyll
You will be shown how to use either a colorimeter or a
spectrophotometer to measure the absorption spectrum of your
extract. Once you have done this you will need to plot a graph of
absorption against wavelength for the data you collected. Compare
this to the absorption and action spectrums of chlorophyll. How
good is your match and how could the experiment be altered to
improve your results.