Guo-Page 1 of 26

Lian-Wang Guo, MS, PhD Davis Heart and Lung Research Institute, 611B The Ohio State University 473 West 12th Avenue, Columbus, OH 43210 Phone: 614-292-5276. Cell: 608-209-2645 Email: Lianwang.Guo@osumc.edu Current appointments 03/17-present Associate Professor, Department of Surgery (70% appointment), College of Medicine, The Ohio

State University

03/17-present Associate Professor, Department of Physiology and Cell Biology (30% appointment), The Ohio State University

02/17-present Investigator, Davis Heart and Lung Research Institute, Wexner Medical Center, The Ohio State University

Academic Appointments 02/17-03/17 Visiting Associate Professor, Department of Surgery, Department of Physiology and Cell Biology,

College of Medicine; Davis Heart and Lung Institute, The Ohio State University, Columbus, OH

04/13-01/17 Assistant Professor (Faculty), Division of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI

04/15-01/17 Faculty Research Scientist (WOC), William S. Middleton Memorial Veterans Hospital, Madison, WI

02/12-03/13 Senior Scientist, Department of Surgery, School of Medicine and Public Health, University of Wisconsin-Madison, WI

08/08-01/12 Senior Scientist, Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, WI

08/04-07/08 Associate Scientist, Department of Pharmacology, School of Medicine and Public Health, University of Wisconsin-Madison, WI

05/00-07/04 Assistant Scientist, Department of Pharmacology, Medical School, University of Wisconsin, WI

09/98-04/00 Assistant Researcher, Department of Microbiology, University of Wisconsin, WI

05/95-04/96 Research Scientist, Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, China Other Appointments 04/2017- present Trainer, the Biomedical Sciences Graduate Program, The Ohio State University, Columbus, OH.

04/2017- present Trainer, the Molecular, Cellular, and Developmental Biology Graduate Program, The Ohio State University, Columbus, OH.

2008-present Member, McPherson Eye Research Institute, University of Wisconsin-Madison, WI (I have remained an associate member after relocation to OSU in 2017) 2014-2017 Trainer, University of Wisconsin Cellular and Molecular Pathology Graduate Training Program,

Madison, WI. (My PhD student Mengxue Zhang is graduating from the CMP program) 2014-2017 Trainer, University of Wisconsin Ophthalmology and Vision Science NIH T32 Research Training

Program, Madison, WI 2014-2017 Trainer, University of Wisconsin-Madison Vascular Surgery NIH T32 Research Training Program,

WI 2014-2017 Member, Cardiovascular Research Center, University of Wisconsin-Madison, WI 2013-2017 Member, Stem Cells & Regenerative Medicine Center, University of Wisconsin-Madison, WI 2014-2017 Member, Institute for Clinical and Translational Research, University of Wisconsin-Madison, WI Degrees 05/96-05/98 Postdoctoral Fellow, Depts of Genetics/Biochemistry, the Hebrew University of Jerusalem, Israel 09/92-05/95 Ph.D., plant physiology and biochemistry, Shanghai Institute of Plant Physiology, Chinese

Academy of Sciences, Shanghai, China 09/89-05/92 M.Sc., plant physiology, Shanghai Institute of Plant Physiology, Chinese Academy of Sciences 09/85-05/89 B.Sc., biology, Xinjiang University, Urumqi, China

Fellowships, Internships, Residency None

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RESEARCH 1) List of Books, Articles, and Other Published Papers.

a) Books (Other than Edited Volumes) None b) Edited Books None c) Chapters in Edited Books Zhang M, Wang B, Kent KC, Guo L-W* (corresponding author). Vascular fibrosis and disease. M. S. Willis et al. (eds.), Fibrosis in Disease. Molecular and Translational Medicine (Springer Nature Switzerland) (2019) 14:369-386 20% authorship: made major revisions, finalized manuscript, and corrected proof. d) Bulletins, Tech Reports, Fact Sheets None e) Peer-Reviewed Journal Articles (‡ indicates equal contribution * indicates corresponding author) 1. Yang H, Shen H, Li J, Guo L-W* (corresponding author). Sigma-1 receptor ablation impairs autopahgosome

clearance. Autophagy (Impact Factor:11). 14: 1-19 (2019). PMC6693456 30% authorship: co-designed experiments, analyzed data, wrote manuscript, made revisions, answered

reviewers’ questions, and corrected proof. 2. Wang B, Zhang M, Urabe G, Chen G, Wheeler D, Dornbos D, Huttinger A, Huang Y, Nimjee S, Gong S, Guo L-

W* (co-corresponding author), Kent KC*. PERK inhibition mitigates restenosis and thrombosis - a potential low-thrombogenic anti-restenotic paradigm. J Am Coll Cardiol Basic to Translational Science (JACC new journal, expected IF: ~8) (2019). Accepted. Biorxiv preprint doi: https://doi.org/10.1101/581397.

15% authorship: co-designed experiments, analyzed data, co-wrote the manuscript, made revisions, and answered reviewers’ questions.

3. Li J, Urabe G, Zhang M, Huang Y, Wang B, Marcho L, Shen H, Kent KC, Guo L-W* (corresponding author). A

role for polo-like kinase-4 in adventitial fibroblast cell type transition. J Am Coll Cardiol Basic to Translational Science (JACC new journal, expected IF: ~8) (2019). In revision. BioRxiv Preprint, doi: https://doi.org/10.1101/570267

15% authorship: designed experiments, analyzed data, wrote the manuscript, making revisions and answering reviewers’ questions.

4. Xie X, Urabe G, Marcho L, Stratton M, Guo L-W* (co-corresponding author), Kent KC*. ALDH1A3 regulations of

matricellular proteins promote vascular smooth muscle cell phenotypic transition. iScience (a Cell Press new journal; IF yet to be available). 19:872-882 (2019) PMC6739626

20% authorship: co-designed experiments (with Dr. Xie), co-analyzed data, co-wrote manuscript, made revisions, answered reviewers’ questions, and finalized the manuscript.

5. Zhang J, McIntosh BE, Wang B, Brown M, Probasco MD, Webster S, Duffin B, Zhou Y, Guo L-W, Burlingham

WJ, Kent KC, Ferris M, and Thomson JA. A Human Pluripotent Stem Cell-Based Screen for Smooth Muscle Cell

Published work type Number of publications

Books 0 Edited Books 0

Chapters In Edited Books 1 Bulletins, Tech Reports, Fact Sheets 0 Peer-Reviewed Journal Articles 68 Editor-Reviewed Journal Article 0 Other Journal Articles 0 Reviews 6 Abstract and Short Entries 0 Papers In Proceedings 0 Total 75

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Differentiation and Maturation Identifies Inhibitors of Intimal Hyperplasia. Stem Cell Reports (IF: 6.5) 12: 1269-1281 (2019) PMC6565755

10% authorship: co-designed experiments and analyzed partial data. 6. Zhang M, Wang B, Urabe G, Huang Y, Plutzky J, Kent KC, Guo L-W* (corresponding author). The BD2 domain of

BRD4 is a determinant in EndoMT and vein graft neointima formation. Cellular Signaling (IF: 4) 61:20-29 (2019) PMID3107539. Citation count: 2

30% authorship: co-designed experiments, analyzed data, wrote manuscript, made revisions, answered reviewers’ questions, and corrected proof.

7. Shi Y, Johnson J, Wang B, Chen B, Fisher GL, Urabe G, Shi X, Kent KC, Guo L-W* (co-corresponding author), Li

L*. Mass Spectrometric Imaging Reveals Temporal and Spatial Dynamics of Bioactive Lipids in Arteries Undergoing Restenosis. J Proteome Research (IF: 3.8) 18:1669-1678 (2019) PMC6481175. Citation count: 3

Featuring suppl cover art of the journal at https://pubs.acs.org/toc/jprobs/18/4 15% authorship: initiated collaboration, co-designed experiments, analyzed data, wrote manuscript, made

revisions, answered reviewers’ questions, and corrected proof. 8. Zhang M, Urabe G, Little C, Wang B, Kent AM, Huang Y, Kent KC, Guo L-W* (corresponding author). HDAC6

Regulates the MRTF-A/SRF Axis and Vascular Smooth Muscle Cell plasticity. J Am Coll Cardiol Basic to Translational Science (expected impact factor ~8) (2019). 3(6):782–95. PMC6314972. Citation count: 3

25% authorship: designed experiments, analyzed data, wrote manuscript, made revisions, answered reviewers’ questions, and corrected proof.

9. Wang B, Chen G, Urabe G, Xie R, Wang Y, Shi X, Guo L-W* (co-corresponding author), Gong S*, Kent KC*. A

paradigm of endothelium-protective and stent-free anti-restenotic therapy using biomimetic nanoclusters. Biomaterials (IF: 10.3) 178:293-301 (2018). PMC6082402. Google citation count: 23

20% authorship: coordinated collaboration, co-designed experiments, analyzed data, co-wrote manuscript, made revisions, answered reviewers’ questions, and corrected proof.

10. Pan X, Wang B (equal first authors), Zhang M, Yuan T, Kent KC, Guo L-W* (corresponding author). Analysis of

combined transcriptomes identifies gene modules that differentially respond to pathogenic stimulation of vascular smooth muscle and endothelial cells. Scientific Reports (Nature Publishing Group) 8:395 (2018). PMC5762668

20% authorship: co-designed experiments, analyzed data, wrote manuscript, made revisions, answered reviewers’ questions, and corrected proof.

11. Mavlyutov TA, Chen X, Guo L-W, and Yang J. APEX2 tagging of the sigma-1 receptor indicates subcellular

protein topology with cytosolic N-terminus and ER luminal C-terminus. Protein Cell (Springer-Nature, IF: 7.6) 9:7330737 (2018) PMC6053353. Citation count: 13

20% authorship: co-designed experiments, analyzed data, co-wrote manuscript, made revisions, and corrected proof.

12. Yang H, Fu Y, Liu X, Shahi P, Mavlyutov, Li J, Yao A, Guo S, Pattnaik BR, Guo L-W* (corresponding author).

Role of the sigma-1 receptor chaperone in rod and cone photoreceptor degenerations in a mouse model of retinitis pigmentosa. Molecular Neurodegenration (IF: 8.3) 12:68. (2017) PMC5606113. Citation count: 9

20% authorship: initiated collaboration, designed experiments, analyzed data, wrote manuscript, made revisions, answered reviewers’ questions, and corrected proof.

13. Liu X, Fu Y, Yang H, McCurdy C, Guo L-W* (co-corresponding author), Pattnaik B. Potential independent action

of sigma Receptor ligands through inhibition of the Kv2.1 channel. Oncotarget 8:59345-59358 (2017) PMC5601737. Citation count: 6

15% authorship: initiated and coordinated collaboration, co-designed experiments, analyzed data, wrote the majority of the manuscript, made revisions, answered reviewers’ questions, and corrected proof.

14. Chen G, Shi X, Wang B, Xie R, Guo L-W* (co-corresponding author), Gong S, Kent KC. A unimolecular micelle-

based hybrid system for perivascular drug delivery produces long-term efficacy for neointima attenuation in rats. Biomacromolecules 18: 2205-2213 (impact factor: 5.7) (2017) PMC5927366. Citation count: 13

15% authorship: coordinated collaboration, co-designed experiments, analyzed data, wrote the majority of the manuscript, made revisions, answered reviewers’ questions, and corrected proof.

15. Mavlyutov TA, Yang H, Epstein ML, Ruoho AE, Yang J, Guo L-W* (corresponding author). APEX2-enhanced

electron microscopy distinguishes sigma-1 receptor localization in the nucleoplasmic reticulum. Oncotarget (2017) PMID 28572528. Citation count: 10

25% authorship: designed experiments, analyzed data, wrote and finalized manuscript, made revisions, answered reviewers’ questions, and corrected proof.

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16. Zhao L, Li J, Fu Y, Zhang M, Wang B, Ouellette J, Shahi PK, Pattnaik BR, Watters JJ, Wong WT, Guo L-W*

(Corresponding author). Photoreceptor protection via blockade of BET epigenetic readers in a murine model of inherited retinal degenration. J Neuroinflammation (IF: 5.7) 14:14. (2017). PMC5248448. Citation count: 4

20% authorship: initiated and coordinated collaborations, co-designed experiments, analyzed data, wrote and finalized manuscript, made revisions, answered reviewers’ questions, and corrected proof.

17. Yu Q, Wang B, Chen Z, Urabe G, Glover MS, Shi X, Guo L-W, Kent KC, Li L. Electron-Transfer/Higher-Energy

Collision Dissociation (EThcD)-Enabled Intact Glycopeptide/Glycoproteome Characterization. J. Am. Soc. Mass Spectrom. (IF: 3.2) (2017) PMC5711575. Citation count: 40

10% authorship: coordinated collaboration, co-designed experiments, analyzed partial data, critically reviewed the manuscript, made revisions, and corrected proof.

18. Li J, Zhao L, Urabe G, Fu Y, Guo L-W* (corresponding author). Epigenetic intervention with a BET inhibitor

ameliorates acute retinal ganglion cell death in mice. Molecular Vision 23:149-159 (2017) PMC5360452. Citation count: 6

30% authorship: designed experiments, analyzed data, wrote manuscript, made revisions, answered reviewers’ questions, and corrected proof.

19. Kumar A, D’Souza SS, Moskvin OV, Toh H, Wang B, Zhang J, Swanson S, Guo L-W, Thomson JA, Slukvin II.

Specification and diversification of Pericytes and smooth muscle cells from mesenchymoangioblasts. Cell Reports (IF: 7.8) 19:1902-16 (2017) PMC6428685. Citation count: 35

10% authorship: coordinated collaboration, designed partial experiments, critically reviewed the manuscript. 20. Zhao L, Chen G, Li J, Fu Y, Mavlyutov TA, Yao A, Nickells RW, Gong S, Guo L-W* (Corresponding author). An

intraocular drug delivery system using targeted nanocarriers attenuates retinal ganglion cell degeneration. Journal of Controlled Release (IF: 7.9) 247:153-166 (2017). PMC5323250. Citation count: 26

25% authorship: coordinated collaborations, co-designed experiments, analyzed data, wrote the majority of the manuscript, made revisions, answered reviewers’ questions, and corrected proof.

21. Zhu Y, Takayama T, Kent A, Wang B, Zhang M, Binder B, Goel S, Zhou Y, Little C, DiRenzo D, Roenneburg DA,

Shi X, Murphy WL, Kent KC, Ke J, Guo L-W* (corresponding author). Restenosis inhibition and in vitro re-differentiation of TGFb/Smad3-activated smooth muscle cells by resveratrol. Scientific Reports (Nature Publishing Group) 7:41916 (2017). PMC5292946. Citation count: 11

25% authorship: coordinated collaboration, designed experiments, analyzed data, wrote manuscript, made revisions, answered reviewers’ questions, and corrected proof.

22. Shi XD‡, Guo L-W‡*(equal first author, co-corresponding author), Seedial SM‡, Wang B, Takayama T, Franco S, DiRenzo DM, Bo Liu, Kent KC. Local CXCR4 induction in the injured arterial wall contributes to intimal hyperplasia. Stem Cells (5-year Impact factor 7.5) (2016) PMC5113668. Citation count: 11

23. DiRenzo DM, Chaudhary MA, Shi XD, Franco SR, Zent J, Wang K, Guo L-W* (co-corresponding author), Kent

KC. A crosstalk between TGF-β/Smad3 and Wnt/β-catenin pathways promotes vascular smooth muscle cell proliferation. Cellular Signaling 28(5): 498-505. (2016). PMC4788971. Citation: 37

24. Abate C, Riganti C, Pati ML, Ghigo D, Berardi F, Mavlyutov TA, Guo L-W, Ruoho AE. Development of Sigma-1

receptor fluorescent ligands as versatile tools to study Sigma-1 receptors. European Journal of Medicinal Chemistry 108:577-85 (2016) PMC4755300

25. Wang B, Zhang M, Takayama T, Shi XD, Roenneburg D, Kent KC, Guo L-W* (Corresponding author). BET

bromodomain blockade mitigates intimal hyperplasia in rat carotid arteries. EBioMedicine (a new Cell Press/Lancet journal, IF 6.7) 2:1650-61 (2015) PMC4740308. Citation count: 23

Featuring a commentary by Dr. Katherine Martin (PMC4740337) specific on our paper 26. Chu UB, Mavlyutov TA, Yang H, Chu M-L, Schulman A, Mesangeau C, McCurdy CR, Guo L-W* (co-

corresponding author), Ruoho AE*. The Sigma-2 receptor and progesterone receptor membrane component 1 are different binding sites derived from independent genes. EBioMedicine (a new Cell Press/Lancet journal) 2:1806-1813 (2015) PMC4740303. Citation count: 57

27. Tsai T-L, Wang B, Squire MW, Guo L-W, Li Wan-Ju. Endothelial Cells Direct Human Mesenchymal Stem Cells

for Osteo- and Chondro-lineage Differentiation through Endothelin-1 and AKT Signaling. Stem Cell Research & Therapy. (IF: 4.6) 6:88 (2015) PMC4416238. Citation count: 28

This work won the Orthopedic Research Society New Investigator Recognition Award, Feb, 2015

Page 5 of 24

Awardee: Wan-Ju Li (collaborator) 28. Mavlyutov TA, Epstein M, Guo L-W* (corresponding author). Subcellular Localization of the Sigma-1 Receptor in

Retinal Neurons — an Electron Microscopy Study. Scientific Reports (Nature Publishing Group) 5:10689. (2015) PMC4649997. Citation count: 37

29. Takayama T, Shi XD, Wang B, Franco S, Zhou Y, DiRenzo D, Kent A, Hartig P, Zent, J, Guo L-W*

(corresponding author). A murine model of arterial restenosis after angioplasty: technical aspects of wire injury of mouse fenoral artery. Journal of Visual Experiments (97), e52561, (2015). PMC4401250. Citation count: 6

30. Mavlyutov TA, Guo L-W, Epstein M, Ruoho AE. Role of the sigma-1 receptor in amyotrophic lateral sclerosis

(ALS). Journal of Pharmocological Sciences. 127:10-16 (2015) PMC4489701 31. Goel SA‡, Guo L-W‡* (equal first author, corresponding author), Wang B, Song Guo, Drew Roenneburg, Gene

Ananiev, F. Michael Hoffmann, K. Craig Kent. High-throughput Screening Identifies Idarubicin as a Preferential Inhibitor of Smooth Muscle versus Endothelial Cell Proliferation. PloS ONE 9:e89349 (2014). PMC3933427. Citation count: 12

32. Shi XD, Chen GJ, Guo L-W*, Si Y, Zhu M, Pilla S, Liu B, Gong SQ, Kent KC. Periadventitial application of

rapamycin-loaded nanoparticles produces sustained inhibition of vascular restenosis. PloS ONE 9(2):e89227 (2014). PMC3931710. Citation count: 30

33. Shi XD‡, Guo L-W‡*(equal first author, corresponding author), Seedial SM‡, Si Y, Wang B, Takayama T,

Suwanabol PA, Ghosh, S, DiRenzo D, Liu B, Kent KC. TGF-β/Smad3 inhibit vascular smooth muscle cell apoptosis through an autocrine signaling mechanism involving VEGF-A. Cell Death & Disease (Nature Publishing Group, Impact factor 6.0) 5:e1317 (2014). PMC4123076. Citation count: 33

34. Yu X‡, Takayama T‡, Goel SA, Shi XD, Zhou Y, Kent KC, Murphy WL, Guo L-W* (corresponding author). A

rapamycin-releasing perivascular polymeric sheath produces highly effective inhibition of intimal hyperplasia. Journal of Controlled Release (IF: 7.9) 191:47-53. (2014). PMC4156896. Citation count: 20

35. Shi XD‡, DiRenzo D‡, Guo L-W‡* (equal first author, corresponding author), Franco S, Wang BW, Seedial S,

Kent KC*. TGF-β/Smad3 Stimulates Stem Cell/Developmental Gene Expression and Vascular Smooth Muscle Cell De-Differentiation. PloS ONE. 9(4):e 93995 (2014). PMC3981734. Citation count: 30

36. Guo L-W* (corresponding author), Wang B, Goel SA, Takayama T, Little C, Roenneburg D, Kent KC.

Halofuginone stimulates adaptive remodeling and preserves re-endothelialization in balloon-injured rat carotid arteries. Circulation-Cardiovascular Intervention (Impact factor 6.9) 7(4): 594-601 (2014). PMC4140988. Citation count: 16

37. Goel SA‡, Guo L-W‡* (equal first author, corresponding author), Shi X-D, Kundi R, Sovinsky G, Seedial S, Liu

B, Kent KC. Preferential secretion of collagen type 3 versus type 1 from adventitial fibroblasts stimulated by TGF-β/Smad3-treated medial smooth muscle cells. Cellular Signaling. 2012; 25(4):955-960. PMC3595331. Citation count: 18

38. Guo L-W, Hajipour AR*, Karaoglu K, Mavlyutov T, Ruoho AE. Development of benzophenone-alkyne

bifunctional sigma receptor ligands. ChemBioChem 13(15):2277-89. (2012). PMC3517089 39. Hajipour AR, Guo L-W*, Pal A, Mavlyutov T, Ruoho AE Electron-donating para-methoxy converts a benzamide-

isoquinoline derivative into a highly Sigma-2 receptor selective ligand. Bioorg Med Chem. 19:7435-7440. (2011). PMC3229200

40. Mavlyutov TA, Nickells RW, and Guo L-W* (corresponding author). Accelerated ganglion cell death in mice

deficient in the sigma-1 receptor. Molecular Vision 17:1034-43. (2011). PMC3084245 54 Google Scholar citations 41. Guo, L-W* (corresponding author), Ruoho, AE. The N-terminal Half of the PDE6 γ-Subunit Contributes to

Stabilization of the GTPase Accelerating Protein Complex. J Biol Chem 286(17):15260-7. (2011). 42. Chu UB, Song JK, Mavlyutov TA, and Guo L-W* (corresponding author). GTP-dependent interaction of tubulin

with the photoreceptor cGMP phosphodiesterase γ-Subunit. Neuroscience Letters, 482: 225-229. (2010). PMC2963155

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43. Guo L-W* (corresponding author), Hajipour AR, and Ruoho AE. Complementary interactions of the rod cGMP phosphodiesterase inhibitory subunit with the catalytic subunits and transducin. J Biol Chem 285(20):15209-19. (2010). PMC2865329. Citation count: 10

44. Song J‡, Guo L-W‡ (equal first author), Muradov H, Artemyev NO, Ruoho AE, Markley JL. Intrinsically

disordered γ-subunit of cGMP phosphodiesterase encodes functionally relevant transient secondary and tertiary structure. Proc Natl Acad Sci USA 105:1505-1510. (2008). PMC2234174

Google Scholar citation count: 82 45. Pal A, Chu UB, Ramachandran S, Guo L-W, Hajipour AR, and Ruoho AE. Juxtaposition of the steroid binding

domain-like I and II regions constitutes a ligand binding site in the sigma-1 receptor. J Biol Chem 283: 19646-19656. (2008). PMC2443669. Citation count: 54

46. Guo L-W* (corresponding author), Assadi-Porter FM, Grant JE, Wu H, Markley JL, Ruoho AE. One-step purification of bacterially expressed recombinant transducin α-subunit and isotopically labeled PDE6 γ-subunit for NMR analysis. Protein Expression and Purification. 51: 187-197. (2007). PMID: 16938469. Citation count: 20

47. Hajipour AR, Guo L-W, and Ruoho AE. Nitric acid in the presence of supported P2O5 on silica gel affords an

efficient and mild system for oxidation of organic compounds under solvent-free conditions. Molecular Crystals and Liquid Crystals 456:85-93. (2006).

48. Guo L-W* (corresponding author), Muradov H, Hajipour AR, Sievert MK, Artemyev NO, and Ruoho AE. The

inhibitory γ-subunit of the rod cGMP phosphodiesterase binds the catalytic subunits in an extended linear structure. J Biol Chem 281: 15412-15422. (2006). PMID: 16595671. Citation count: 42

40 Google Scholar citations 49. Grant JE, Guo L-W, Vestling MM, Martemyanov KA, Arshavsky VY, and Ruoho AE. The N-terminus of GTPγS-

activated transducin α-subunit interacts with the C-terminus of cGMP phosphodiesterase γ-subunit. J Biol Chem 281:6194-6202. (2006). PMID: 16407279. Citation count: 27

50. Guo L-W* (corresponding author) and Ruoho AE. Inhibition of cGMP-dependent protein kinase by the regulatory

subunit of photoreceptor cGMP phosphodiesterase. Neurosci Lett 401:252-255. (2006). PMID: 16603314 51. Geiser AH, Sievert MK, Guo L-W* (corresponding author), Grant JE, Krebs MP, Fotiadis D, Engel A, and Ruoho

AE. Bacteriorhodopsin chimeras containing the third cytoplasmic loop of bovine rhodopsin activate transducin for GTP/GDP exchange. Protein Science 15: 1679-1690. (2006). PMID: 16815918

52. Guo L-W*, Grant JE, Hajipour AR, Muradov H, Arbabian M, Artemyev NO, and Ruoho AE. Asymmetric

interaction between rod cyclic GMP phosphodiesterase γ-subunits and αβ-subunits. J Biol Chem 280:12585-12592. (2005). PMID: 15668239. Citation count: 36

53. Guo L-W* (corresponding author), Hajipour AR, Gavala ML, Arbabian M, Martemyanov KA, Arshavsky VY, and

Ruoho AE. Sulfhydryl-reactive, cleavable, and radioiodinatable benzophenone photoprobes for study of protein-protein interaction. Bioconjug Chem 16:685-693. (2005). PMID: 15898738. Citation count: 28

54. Soriano GM, Guo L-W, de Vitry C, Kallas T, Cramer WA. Electron transfer from the Rieske iron-sulfur protein

(ISP) to cytochrome f in vitro: Is a guided trajectory of the ISP is necessary for competent docking. J Biol Chem 277(44): 41865-41871, 2002. PMID: 12207018

55. Tang RH, Guo L-W, Chen GY, Li LR. Effect of double atmospheric CO2 concentration on rice photosynthesis

and Rubisco. Acta Phytophysiologica Sinica 24: 309-312, 1998.

56. Guo L-W, Rubinstein A, Zeligson S, Ohad I, Hirschberg J. Mutations of N247 in the D1 polypeptide of Synechocystis PCC 6803 affect pH dependence of electron transfer in PSII. Proceedings of the XI International Congress on Photosynthesis, p.931, 1998.

57. Guo L-W, Shen YK. Protective mechanisms against photodamage in photosynthetic apparatus of higher plants.

Plant Physiology Journal (former Plant Physiology Communications) 32(1): 1-8, 1996. Citation count: 16 58. Guo L-W, Xu DQ, Shen YK. Photoinhibition of photosynthesis without net loss of D1 protein in wheat leaves

under field conditions. Acta Botanica Sinica (now Journal of Integrative Plant Biology, impact factor 3.7) 38(3): 196-202, 1996. Citation count: 17

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59. Pei ZM‡, Guo L-W‡, Yang GP. Function of ion channel in the photosynthetic energy conversion of higher plants. Science China-Life Sciences 26(1): 38-46, 1996. (Impact factor: 2.3)

60. Pei ZM‡, Fu W‡, Guo L-W‡, Tang ZC. Effect of salt stress on photosystem II heterogeneity in wheat leaves.

Science China-Life Sciences 39(5): 501-510, 1996. (Impact factor: 2.3) 61. Wu H, Zhang S, Xu D, Guo LW, Shen Y. Diurnal and seasonal variations of non-photochemical quenching of

chlorophyll fluorescence in Sweet Viburnum leaves. Acta Phytophysiologica Sinica 23(2):145-150, 1996 62. Guo L-W, Shen YK, Wu H, et al. Studies on photosynthesis of Eucommia. Acta Botanica Sinica (now Journal of

Integrative Plant Biology, impact factor 3.7) 38(4): 283-286, 1995. 63. Guo L-W, Xu DQ, Shen YK. Protective effect of photorespiration against photoinhibition in cotton leaves. In:

Mathis P (ed.) Photosynthesis: from Light to Biosphere. Kluwer Academic Publishers, Netherland, Vol. IV, 195-198, 1995.

64. Guo L-W, Xu DQ, Shen YK. Relation of photorespiration and photoinhibition of photosynthesis in cotton leaves. Chinese Science Bulletin 40(20): 1885-1888, 1995. Impact factor: 1.6

65. Guo L-W, Wu H, Shen YK et al. Photoinhibition of photosynthesis in some alpine meadow plants. Alpine

Meadow Ecosystem. Science Press, Beijing, Vol. 4, pp. 23-28, 1995. 66. Guo L-W, Zhang Y, Shen YK. Study on the distribution of photosynthesis inhibitor fed through the leaves. Plant

Physiology Journal (formerly Plant Physiology Communications) 30: 360-362, 1994. 67. Guo L-W, Xu DQ, Shen YK. The causes of midday decline of photosynthetic efficiency in cotton leaves under

field conditions. Acta Phytophysiologica Sinica 20(4): 360-366, 1994. Citation count: 26 68. Guo L-W, Xu DQ. Photoinhibition of photosynthesis in sweet viburnum leaves under natural conditions. Acta

Phytophysiologica Sinica 20(1): 46-54, 1993. Citation count: 11

Complete List of Published Work in MyBibliography http://www.ncbi.nlm.nih.gov/sites/myncbi/1H9SgvqKd9xkJ/bibliography/47258922/public/?sort=date&direction=descending

https://www.ncbi.nlm.nih.gov/pubmed/?term=lian-wang+guo+OR+lianwang+Guo The earlier publications are not included in MyBibliography because they were published in plant biology journals.

f) Editor-Reviewed Journal Articles None g) Other Journal Articles None h) Reviews (All Peer Reviewed) 69. Zent J, Guo L-W* (corresponding author). TGFbeta and extracellular matrix-associated signal transduction in

driving myofibroblast differentiation. Cellular Signaling. (2019) Minor revision 50% authorship: co-wrote and finalized the manuscript and made revisions. 70. Zent J, Guo L-W* (corresponding author). Signaling mechanisms of myofibroblastic activation: outside-in and

inside-out. Cellular Physiology and Biochemistry. 49:848-868 (2018) PMID:30184544. Citation count: 7 50% authorship: co-wrote and finalized the manuscript, made revisions, answered reviewers’ questions, and

corrected proof. 71. Mavlyutov TA, Guo L-W* (corresponding author). Peeking into sigma-1 receptor functions through the retina.

Adv Exp Med Biol. (2017) 964: 285-297. PMC6283661. Citation count: 5 50% authorship: co-wrote and finalized the manuscript, made revisions, answered reviewers’ questions, and corrected proof.

72. Chaudhary MA‡, Guo L-W‡* (equal first author, co-corresponding author), Shi X, Chen G, Gong S, Liu B, Kent

KC*. Periadventitial Drug Delivery for the Prevention of Intimal Hyperplasia Following Open Surgery. Journal of Controlled Release (IF: 7.9) 233:174-180 (2016) PMID:27179635. Citation count: 17

73. Goel SA‡, Guo LW‡* (equal first author, co-corresponding author), Liu B, Kent KC. Mechanisms of post-intervention arterial remodeling. Cardiovasc Res. (IF: 7.0) 96(3):363-71. (2012). PMC3500049

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Featuring “Editor’s choice”, 70 Google Scholar citations 74. Guo L-W* (corresponding author) and Ruoho AE. The retinal photoreceptor cGMP phosphodiesterase γ-

subunit—A chameleon. Invited review in Current Protein and Peptide Science 9:611-625. (2008). PMC3219533 i) Abstract and Short Entries (Indicate Whether Peer Reviewed) None j) Papers in Proceedings (Indicate Whether Peer Reviewed) None k) Potential publications under review (indicate authorship, date of submission, and to what journal or publisher the work has been submitted) 1. Huang Y, Urabe G, Zhang M, Li J, Wang B, Kent KC, Guo L-W* (corresponding author). Nullifying epigenetic writer

DOT1L attenuates intimal hyperplasia. Atherosclerosis (IF: 4) (2019) Under review BioRxiv Preprint, doi: https://doi.org/10.1101/730572

2. Shen H, Li J, Wang B, Plutzky J, Guo L-W* (corresponding author). BRD2 regulates sigma-2 receptor expression in the cholesterol metabolic pathway. Life Science Alliance (Oxford Press new journal, estimated IF >6) In revision BioRxiv Preprint, doi: https://doi.org/10.1101/748236

3. Schmitt HM, Fherman RL, Maes ME, Yang H, Guo L-W, Schlamp CL, Pelzel HR, Nickells RW. Increased susceptibility and intrinsic apoptotic signaling in neurons by forced HDAC3 expression. Molecular Neurodegenration (IF 8.3) Under review

4. Xie X, Urabe G, Huang Y, Guo L-W* (co-corresponding author), Kent KC*. NRP2 mediates Smad3 signaling in smooth muscle cell phenotype transition and intimal hyperplasia. JAHA (IF: 4.6) Under review

5. Wang B, Zhang M, Urabe G, Shirasu T, Guo L-W* (co-corresponding author), Kent KC*. PERK inhibition promotes

post-angioplasty re-endothelialization. Journal of Surgical Research Under review 6. Yodsanit N, Zhao Y, Wang B, Guo L-W* (co-corresponding author), Kent KC*, Gong S*. Versatile Nanoparticles for

Aneurysm Treatment and Imaging. Review paper, to be submitted to Journal of Controlled Release (IF: 7.9)

7. Zhang M, Wang B, Urabe G, Kent KC, Guo L-W* (corresponding author). Methyltransferases EZH1 and EZH2 are determinants of smooth muscle cell’s disease phenotypes and intimal hyperplasia. To be submitted

l) Unpublished Scholarly Presentations (Indicate Whether Peer Reviewed) Invited Oral Presentations International/National 1. Lian-Wang Guo. A tale of a writer and a reader. The 12th Annual Vascular Noninvasive Testing Symposium; All About the

Aorta and Arterial Disease -- A Primer for Primary Care Doctors, Specialists and Affiliated Healthcare Professionals. Sponsored by The Ohio State University Division of Vascular Diseases and Surgery and Peripheral Vascular Lab. Nov 8, 2019, Columbus, OH, USA Presenter: Lian-Wang Guo

2. Lian-Wang Guo. Epigenetic regulations of EZH1/2 in smooth muscle cell phenotype change and restenosis. Vascular Biology 2019 – North American Vascular Biology Organization (NAVBO) Annual Meeting. Oct 27, 2019, Monterey, CA, USA.

Presenter: Lian-Wang Guo 3. Mengxue Zhang, Bowen Wang, Go Urabe, K Craig Kent, Lian-Wang Guo. Methyltransferases EZH1 and EZH2 are

determinants of smooth muscle cell’s disease phenotypes and intimal hyperplasia. The 6th Metabolic Cardiovascular Disease Symposium, satellite to the Arteriosclerosis, Thrombosis and Vascular Biology (ATVB) American Heart Association Scientific Session, Boston, MA, USA. May 13, 2019

Presenter: Mengxue Zhang

4. Bowen Wang, Mengxue Zhang, Xudong Shi, Lian-Wang Guo, Craig Kent. Parthenolide Inhibits Inflammatory

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Dysfunction of Human Aortic Endothelial Cells and Proliferation of Smooth Muscle Cells in vitro and Restenosis in a Rat Model. The Vascular Research Initiatives Conference and Arteriosclerosis, Thrombosis and Vascular Biology (ATVB) 2018 Scientific Sessions. San Franscisco, CA, USA. May 8, 2018

Presenter: Bowen Wang 5. Guo L-W (invited talk). New regulators in neurodegerative and vascular diseases involving autophagy and

epigenetics. The Second Pudong Heath Forum (Nov. 15, 2017) and Panelist Discussion of Strategic Development of the School of Public Health (Nov.16,2017), Fudan University, Shanghai, China. Total 6 scholars were invited from the USA and Canada as speakers and panelists.

Presenter: Lian-Wang Guo 6. Guo L-W. An essential role of the sigma-1 receptor in autophagosome-lysosome fusion. Annual Meeting of the

Association for Research in Vision and Ophthalmology (ARVO). May 6-10th, 2017. Baltimore, MD, USA Presenter: Lian-Wang Guo 7. Wang B, Shi XD, Zhang M, Guo L-W* (co-corresponding author), Kent KC. Targeting the PERK Pathway of ER

Stress Response for Endothelium Protection and Restenosis Prevention: A Paradigm for Developing Anti-thrombogenic stents. Atherosclerosis Thrombosis and Vascular Biology (ATVB) Scientific Sessions. May 5th, 2016. Nashville, TN, USA

Audience: Physicians, medical students, residents, fellows, scientists Presenter: Bowen Wang, PhD student co-mentored by Lian-Wang Guo 8. Shi XD, Wang K, Guo L-W, Wang B, Zhang M, Chaudhary MA, Franco SR, Zhu Y, Kent KC. TGF-β/Smad3

stimulates CD34 expression, MAPK activation and vascular smooth muscle cell proliferation. American College of Surgeons Clinical Congress, Chicago. October 2015.

Audience: Physicians, medical students, residents, fellows, scientists Presenter: Bowen Wang, PhD student co-mentored by Lian-Wang Guo 9. Ruoho AE, Mavlyutov TA, Chu UB, Chu M, Yang H, McCurdy C, Guo L-W. Sigma-2 receptor 18 kDa and

PGRMC1 are distinct gene products.Nanosymposium, Neuroscience Conference, Chicago. Oct.2015. Presenter: Ruoho AE, collaborator 10. Little CJ, Kent A, Wang B, Chaudhary MA, Kent KC, Guo L-W. Apicidin Inhibits Major Vascular Smooth Muscle

Cell Pathogenic Phenotypes. The 11th Annual Academic Surgical Congress. Jacksonville, FL. Feb 2016. Audience: Physicians, medical students, residents, fellows, scientists Presenter: Chris Little, medical student, mentored by Lian-Wang Guo 11. Wang B, Zhang M, Takayama T, Shi XD, Roenneburg D, Kent KC, Guo L-W. Inhibition of BET epigenetic

readers mitigates intimal hyperplasia. American College of Surgeons Clinical Congress, Chicago. October 2015. Audience: Physicians, medical students, residents, fellows, scientists Presenter: Mengxue Zhang, PhD student, mentored by Lian-Wang Guo 12. Chaudhary M, DiRenzo DM, Shi XD, Franco S, Little CJ, Liu B Guo L-W, Kent KC. TGF-β/Smad3 promotes

Smooth Muscle Cell De-differentiation and Proliferation through cross with the Wnt/β-Catenin Pathway. Society of Vascular Surgery (SVS) vascualr Research Initiatives Conference (VRIC), San Francisco, CA, May 2015.

Audience: Physicians, medical students, residents, fellows, scientists, postdocs PhD students Presenter: Mirnal Chaudhary, postdoc, co-mentored by Lian-Wang Guo 13. Guo L-W (presenter). BET bromodomain inhibition mitigates intimal hyperplasia in rat carotid arteries. Society of

Vascular Surgery (SVS) vascualr Research Initiatives Conference (VRIC), San Francisco, CA, May 2015. Audience: Physicians, medical students, residents, fellows, scientists, postdocs PhD students 14. Guo L-W (presenter). Nanoparticles Facilitate Sustained Drug Delivery and Inhibition of Restenosis. Society of

Clinical Surgery Scientific Session, University of Wisconsin, Madison. November 15, 2014. Audience: Physicians, medical students, residents, fellows, scientists, postdocs PhD students 15. Guo L-W (presenter). Local CXCR4 Up-Regulation in the Injured Arterial Wall Contributes to Restensis.

American College of Surgeons Clinical Congress, San Francisco. October 2014. Audience: Physicians, medical students, residents, fellows, scientists

16. Guo L-W (presenter). Halofuginone stimulates adaptive remodeling and re-endothelialization. American College

of Surgeons Clinical Congress, Washington DC. October 2013. Audience: Physicians, medical students, residents, fellows, scientists

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17. Guo L-W. High throughput screening for selective anti-restenosis drugs. American College of Surgeons Clinical Congress, Washington DC. October 2013. Audience: Physicians, medical students, residents, fellows, scientists

Regional 18. Guo L-W (presenter, invited talk). The sigma-1 receptor, an unsung hero in autophagy? Invited by Dr. Sylvia Smith, Regents’ Professor and Chair, Department of Cellular Biology and Anatomy, Co-

Director, James and Jean Culver Vision Discovery Institute, Medical College of Georgia, Augusta University, Augusta, Georgia. Aug 15-16, 2018.

19. Guo L-W (presenter, invited talk). The sigma-1 receptor — a new regulator in autophagy. Invited by Dr. Krzystof Palczewski. Chair of Department of Pharmacology, Case Western Reserve University,

Cleveland, OH, USA. Jan 18-19, 2018. 20. Guo L-W (presenter, invited talk). The bromo and extraterminal family epigenetic regulators – new kids on the

block. Invited from University of Wisconsin to OSU. Invited by Dr. Peter Mohler. The Davis Heart and Lung Institute, The Ohio State University, Columbus, OH, July, 2016.

21. Guo L-W (presenter, invited talk). Phosphodiesterase 6 gamma subunit –a critical player in phototransduction.

University of Alabama at Birmingham Medical School, Birmingham, Alabama, September, 2012 22. Guo L-W (presenter, invited talk). Small is beautiful- phosphodiesterase 6 gamma subunit. Wayne State

University Medical School. Detroit, Michigan, May, 2012 23. Guo L-W (presenter, invited talk). Molecular interactions of phosphodiesterase 6. Oakland University Eye

Research Institute, Rochester, Michigan . June, 2009. Local 24. Guo L-W (presenter, invited talk). BETs in vascular cell and wall pathiobiology. Vascular Surgery Retreat,

Department of Surgery, The Ohio State University. Sept 28, 2018. 25. Guo L-W (presenter, invited talk). Targeting the sigma-1 receptor for retinal protection. UnEYEted: The

Combined Symposium of Ophthalmology Department and College of Optometry. Eye and Ear Institute, The Ohio State University, Columbus, OH. Sept 27, 2018.

26. Guo L-W (presenter, invited talk). Sigma receptors — An Enigma. Research in Progress seminar. The Davis

Heart and Lung Institute, The Ohio State University, Columbus, OH, Dec 1, 2017. 27. Guo L-W (presenter, invited talk). A new autophagy regulator. Local Keynote Speaker at the Davis Heart and

Lung Institute Research Day, The Ohio State University, Columbus, OH, Oct 18, 2017. One invited outside keynote speaker, Dr. William Caterall. Two local keynote speakers: Dr. Lian-Wang Guo and Dr. Chandan Sen.

28. Guo L-W (presenter, invited talk). BET epigenetic readers – new face in vascular biology. The 7th Annual

Research Summit, Department of Surgery, University of Wisconsin, Madison, January, 2016. 29. Guo L-W (presenter, invited talk). Apex-enhanced electron microscopy for studies of the Sigma-1 receptor. Stem Cell and Regenerative Medicine Center (SCRMC) Neuroregeneration Focus Group meeting. University of

Wisconsin-Madison. December 14, 2015. 30. Guo L-W (presenter, invited talk). Perivascular Drug delivery with a Polymeric Sheath Prevents Restenosis.

Research in Progress Seminar Series, Department of Surgery, University of Wisconsin, Madison, WI, November, 2014

31. Guo L-W (presenter, invited talk). Nanoparticles for treating restenosis: sustained and targeted local drug

delivery. Presentation to Wisconsin Partnership Program Education and Research Committee (PERC), December, 2013.

32. Guo L-W (presenter, invited talk). A secret weapon for fighting blindness. Research in Progress seminar series. Department of Surgery, University of Wisconsin, Madison, WI, December, 2013.

33. Guo L-W (presenter, invited talk). The sigma-1 receptor in retinal protection. McPherson Eye Research Institute

“Protecting the Retina” outreach program, University of Wisconsin, Madison. June, 2013.

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34. Guo L-W (presenter, invited talk). Screening for selective anti-restenosis drugs. The 4th Annual Research Summit, Department of Surgery, University of Wisconsin, Madison, January, 2013.

35. Guo L-W (presenter, invited talk). The new use of an ancient drug- a test for amelioration of restenosis.

Research in Progress seminar series, Department of Surgery, University of Wisconsin, Madison, October, 2012. 36. Guo L-W (presenter, invited talk). TGFbeta and Smad3 in restenosis. Presentation for newly enrolled Cellular &

Molecular Pathology PhD students. University of Wisconsin-Madison, September, 2012. 37. Guo L-W (presenter, invited talk). Retinal protection by the Sigma-1 receptor. University of Wisconsin Eye

Research Institute, Madison, April, 2011 38. Guo L-W (presenter, invited talk). A structural and functional view of phosphodiesterase 6 gamma. Promega,

Fitchburg, Wisconsin, January, 2010.

Poster Presentations International/National (peer reviewed) 1. Bowen Wang, Mengxue Zhang, Xudong Shi, Lian-Wang Guo, Craig Kent. Parthenolide Inhibits Inflammatory

Dysfunction of Human Aortic Endothelial Cells and Proliferation of Smooth Muscle Cells in vitro and Restenosis in a Rat Model. Arteriosclerosis, Thrombosis and Vascular Biology (ATVB) 2018 Scientific Sessions. San Franscisco, CA, USA. May, 2018. Presenter: Bowen Wang.

2. Mengxue Zhang, Bowen Wang, Go Urabe, Kent KC, and Lian-Wang Guo. The Bromo and Extraterminal

Domain Protein (BET) Family Drives Endothelial-To-Mesenchymal Transition and Contributes to Vein Graft Stenosis. Arteriosclerosis, Thrombosis and Vascular Biology (ATVB) 2018 Scientific Sessions. San Franscisco, CA, USA. May, 2018. Presenter: Mengxue Zhang.

3. Shi Y, Wang B, Shi XD, Guo L-W, Kent KC, Li LJ. MALDI MS Imaging of N-glycans and peptides from human

FFPE aneurysmal and atherosclerotic tissue sections. 64th ASMS Conference on Mass Spectrometry and Allied Topics, June 3-7th, 2018, San Diego, CA, USA. Presenter: Yatao Shi.

4. Mirnal A Chaudhary, Go Urabe, Alex Hayden, Sarah Franco, Xudong Shi, Lian Wang-Guo, K Craig Kent. The

Role of Lymphocyte Specific Protein-1 in the Phenotypic Switch of Smooth Muscle Cells After Arterial Injury. Arteriosclerosis, Thrombosis and Vascular Biology (ATVB) 2017 Scientific Sessions. Minneapolis, MN, USA. May 2017. Presenter: Mirnal Chaudhary.

5. Zhao L, Chen G, Li J, Fu Y, Mavlyutov TA, Yao A, Nickells RW, Gong S, Guo L-W. An intraocular drug delivery

system using targeted nanocarriers attenuates retinal ganglion cell degeneration. The Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO). May 6-10th, 2017. Baltimore, MD, USA

Presenter: Lian-Wang Guo 6. Glover MS, Yu Q, Wang B, Shi X, Guo L-W, Kent KC, Li LJ. Mass Spectrometry-based Proteomic and PTM

Studies Provide Insight into the Molecular Mechanisms of Restenosis US HUPO 2017 Conference (San Diego, CA), USA. Presenter: Matt Glover

7. Shi Y, Wang B, Shi XD, Guo L-W, Kent KC, Li LJ. Mass spectrometry-based metabolomics for biomarker discovery after angioplasty. 64th ASMS Conference, June 5-9, 2016, San Antonio, Texas, USA

8. Glover MS, Yu Q, Wang B, Shi X, Guo L-W, Kent KC, Li LJ. Quantitative Cardiovascular Proteomics of Injured

Arterial Tissue by Dileu Isobaric Labelling: Toward Novel Therapeutic Targets for Restenosis. 64th ASMS Conference, June 5-9, 2016, San Antonio, Texas, USA

9. Wang B, Shi Y, Shi X, Guo L-W, Kent KC, Li LJ. MALDI Mass Spectrometry Imaging Identifies Lipidomic

Signatures in a Diabetic Rat Model of Vascular Disease: Emerging Tool for Discovery of Inflammation-Related Biomarkers. NHLBI Vascular Inflammation Conference Oct. 24-25, 2016, Bethesda, MD, USA

10. Wang B, Zhang M, Takayama T, Shi XD, Roenneburg D, Kent KC, Guo L-W . BET bromodomain inhibition mitigates intimal hyperplasia in rat carotid arteries. The Vascular Research Initiatives Conference and Arteriosclerosis, Thrombosis and Vascular Biology (ATVB) 2015 Scientific Sessions. San Franscisco, CA. May, 2015

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11. Mavlyutov TA, Epstein M, Guo L-W. Subcellular Localization of the Sigma-1 Receptor in Retinal Neurons — an Electron Microscopy Study. Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO), Denver, CO. May, 2015

12. Yang H, Mavlyutov TA, Guo L-W. Sigma-1 receptor ablation impairs mitophagy in neuronal and non-neuronal

cells. 8th Annual 2015 Midwest Eye Research Symposium. Iowa City, IA. August, 2015 13. Toshio Takayama, Yichen Zhu, Alycia Kent, Bowen Wang, Shakti Goel, Xudong Shi, Daniel DiRenzo, Sarah

Franco, K. Craig Kent, Lian-Wang Guo. Resveratrol inhibits Smooth Muscle Cell De-differentiation and Neointima. American College of Surgeons Clinical Congress. Oct 4-8, 2015, Chicago, IL, USA

14. Shi XD, Chen GJ, Guo L-W, Si Y, Zhu M, Pilla S, Liu B, Gong SQ, Kent KC. Periadventitial Application of

Rapamycin-Loaded Nanoparticles Produces Sustained Inhibition of Vascular Restenosis. The Vascular Research Initiatives Conference and Arteriosclerosis, Thrombosis and Vascular Biology (ATVB) 2014 Scientific Sessions. Toronto, Canada. May, 2014

15. Shi XD, DiRenzo D, Guo L-W, Franco S, Wang BW, Seedial S, Kent KC. TGF-β/Smad3 Stimulates Stem

Cell/Developmental Gene Expression and Vascular Smooth Muscle Cell De-Differentiation. Atherosclerosis Thrombosis and Vascular Biology (ATVB) Scientific Sessions. Washington, DC. May, 2013

16. Chu UB, Mavlyutov, Chu M, Guo L-W, Ruoho AE. The true sigma-2 receptor, would you please stand up? The

Experimental Biology Conference. Washington, DC. May, 2013 17. Guo L-W, Goel SA, Shi X-D, Kundi R, Sovinsky G, Seedial S, Liu B, Kent KC. TGF-beta/Smad3-Treated Smooth

Muscle Cells Enhance the Production of Collagen Type 3 from Adventitial Fibroblasts. Atherosclerosis Thrombosis and Vascular Biology (ATVB) Scientific Sessions. Chicago, IL. May, 2012

18. Guo L-W, Song J, Muradov H, Artemyev NO, Ruoho AE, Markley JL. Solution structure of the intrinsically

disordered γ-subunit of cGMP phosphodiesterase. Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO), Fort Lauderdale, FL. May, 2007

19. Guo L-W, Hajipour AR, Arbabian M, and Ruoho AE. Benzophenone photoprobes for study of protein-protein

interactions. Conference of Experimental Biology. Washington DC. April, 2005 20. Guo L-W, Grant JE, Hajipour AR, Arbabian M, Artemyev NO, and Ruoho AE. Profiling of the interaction between

rod cyclic GMP phosphodiesterase γ-subunits and αβ-subunits. Conference of Experimental Biology. San Diego, CA. April, 2003

Local/The Ohio State University 2017-19 Mentored lab members to present: 2 posters at the OSU Translational Science Symposium (12/3, 2019) 5 posters at the DHLRI Research Day (10/31, 2019)

1 poster at the PCB Research Day (3/9, 2018). 3 posters at the Department of Surgery Research Day (5/30, 2018) 3 posters at the DHLRI Research Day (10/23, 2018) 3 posters at the PCB Research Day (3/10, 2017), 2 posters at the Graduate School Research Day (4/13, 2017), 2 posters at the Dept of Surgery Research Day (6/1, 2017). 3 posters at the DHLRI Research Day (10/18, 2017).

University of Wisconsin-Madison 2013-17. Mentored lab members to present posters at: Annual Department of Surgery Research Summits (total 15 posters in 4 consecutive years) WARF (UW patenting agency) Discovery Challenge Research Symposium (6 posters in 4 years) McPherson Eye Resaerch Institute Poster session (4 posters in 3 years), UM-Madison Cardiovascular Research Center Symposium (total 2 posters), UW-Madison Campuswide Undergraduate Research Day (total 2 posters), UW-Madison

2) List of creative works pertinent to your professional focus. a) Artwork None b) Choreography None

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c) Collections None d) Compositions None e) Curated Exhibits None f) Exhibited Artworks None g) Inventions and Patent Applications (All inventors are equal contributors) 1. Xie X, Guo L-W, Urabe G, Kent KC. Repurposing disulfiram for treating cardiovascular disease. Application No.

62/793,521. Filing date: Jan. 17, 2019. Ref# P2019-174-4936. Pending

2. Kent KC, Guo L-W, Wang B, Urabe G, Gong SQ, Chen GJ. BIOMIMETIC VESICLES AND USES THEREOF. Application No.62/675,744. Filing date: May 23, 2018. OSU Ref No.: T2017-369. Pending

3. Kent KC, Guo L-W, Gong SQ, Shi XD, Chen GJ. Perivascular drug delivery system. (P160359US01, USSN:

62/375387) 2017. Pending

4. Guo L-W, Kent KC, Yu XH, Murphy W. PERIVASCULAR DELIVERY SYSTEM AND METHOD. 2015, provisional patent application submitted by WARF. Pending

5. Guo L-W, Kent KC, Goel SA, Hoffmann FM, Takayama T. ANTI-RESTENOSIS COMPOSITIONS AND

METHODS. 2015, provisional patent application submitted by WARF. Pending

6. Goel SA, Guo L-W, Kent KC. TEST OF INSULIN AS A DRUG TO REDUCE RESTENOSIS OF VESSELS (US 9,132,171B2. Sep. 15, 2015) Approved

h) Moving Image None i) Multimedia / Database / Website None j) Radio and Television None k) Recitals and Performances None l) Recordings None m) Other Creative Works None 3) Brief Description of the Focus of the Candidate’s Research, Scholarly or Creative Work, Major Accomplishments, and Plans for the Future, including Works in Progress My research features trajectories of investigation -> intervention -> translation that criss-cross two (vascular and retinal) fields. The major vascular problem I am tackling is blood vessel narrowing that obstructs circulation thus leading to life-threatening cardiovascular diseases. When the heart vitality-maintaining artery is clogged by cholesterol-rich plaques, two major clinical procedures are performed to recanalize the vessel; i.e. grafting a vein to replace the occluded artery or inflating a balloon to press-open the lumen (so-called angioplasty). In either case, the treatment will eventually fail because of the vessel wall thickening that blocks blood flow again, a pathology termed re-stenosis. For the former case, my team is testing a perivascular treatment strategy, that is, to apply drug-carrying nanoparticles to the outside of the vein graft to prevent restenosis. For the latter, we are developing a new paradigm to deliver anti-restenotic drug to the pathologic site using biomimetic nanoparticles that can be conveniently I.V. injected thus obviating the use of expensive and invasive metal stents. I have played an instrumental role in the conception of these innovations, formation of collaborations, and writing of two winning R01 grants to support the respective projects. Though anti-restenotic therapeutics exist in the clinic, e.g. paclitaxel coated on a stent implanted following angioplasty, an inherent problem of these therapeutics is their thrombogenicity that incurs risks of heart failure and stroke in patients. My basic research has identified the BET family proteins as pathogenic determinants in restenosis, which are druggable and hence represent novel anti-restenotic interventional targets. Serendipitously, BET inhibition produced endothelial-protective (anti-thrombogenic) effects in addition to restenosis mitigation in our studies using preclinical models. Further developments based on this breakthrough may lead to epigenetic interventions that, whereas under-explored in the vascular field, could potentially overcome drawbacks of current therapeutic methods.

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As such, BET pathobiology has become a unique niche for my scientific contribution to the field of vascular disease and intervention. Therefore, my studies on BET-dominated restenostic mechanisms followed by their interventional targeting, e.g. applying nanotechnology for precision drug delivery as narrated above, is anticipated to make significant impacts on both BET biology and translational medicine. Taken together, the research team under my leadership have uncovered novel epigenetic mechanisms underlying restenosis. Further, by targeting the BET epigenetic readers, we were able to achieve non-thrombogenic mitigation of restenosis (in rat models) utilizing the lesion-homing property of a biomimetic nanoplatform for drug delivery. This new treatment paradigm represents an important improvement over the thrombogenic drug-coated stents which are the status quo method for treating post-angioplasty restenosis. An NHLBI R01 award has been secured to further develop this project. On the other front, we created a nanoparticle/hydrogel hybrid platform for prevention of (re)stenosis in vein grafts through a perivascular route. These two inventions are poised to solve the recurrent occluding problems that occur respectively following the two major clinical procedures (angioplasty and vein grafting). Patents on two respective prototype products (Pericell and NanoPlatyx1) have been filed, which facilitated our success in obtaining funds to accelerate the translation toward human trial and commercialization. In parallel, the discovery (in my lab) of a novel function of the sigma-1 receptor in autophagy solidifies the cytoprotective role of this drug-binding site. Indeed, using neuron-targeting nanoparticles to deliver a sigma-1 receptor agonist we were able to effectively slow down retinal degeneration in a mouse model of glaucoma, the No.2 blinding disease in developed countries. Furthermore, my lab was the first to identify beneficial effects of inhibiting BET epigenetic readers in retinal degeneration models. As such, my research has come full circle, from retinal neuroscience to vascular pathobiology to retinal protection, as integrated by two potential therapeutic targets (BETs and the sigma-1 receptor). In summary, my basic research to investigate a pathogenic epigenetic reader family and a cytoprotective pathway has uncovered novel molecular/cellular mechanisms. The follow-up translational efforts have yielded favorable therapeutic outcomes in preclinical models. While these research outcomes are leading a way toward potential benefits for patients, I will continue to explore the epigenetic players associated with BETs (e.g. HDAC6, EZH1/2) and the sigma-2 receptor which is functionally coupled with the sigma-1 receptor. I thus envision that given opportunities, new knowledge gained on these drug targets will significantly impact basic science and therapy. 4) Description of Quality Indicators of Your Research, Scholarly or Creative Work, Such as Citations, Publication Outlet Quality Indicators Such as Acceptance Rates, Rankings or Impact Factors of Journal or Publisher As indicated by the Google Scholar data, in the past 5 years (since 2014), 27 of my publication were cited at least 10 times (i10-index). Since 2012, 14 papers were cited at least 20 times; 8 of my publications (all time) have been cited over 40 times. The data also show that most of my publications and their citations occurred after 2014, indicating a fast-growth phase of my productivity. In addition, 10 of my publications since 2017 are in high-impact journals (impact factor >6). 5) Research Funding a) Funded Research, including Contracts and Clinical Trials, on Which the Candidate is or has been the Principal Investigator

NIH R01EY029809 Lian-Wang Guo (PI) 04/01/19-03/31/23 $256,031 annual direct cost “Master epigenetic regulators and retinal degenerative disease” Role: PI Goal: When innate immune cells go rogue they become culprits of retinal neuronal death. The objective of this project is to identify a master epigenetic switch governing the retinal microglial transition into a detrimental state, so as to establish an interventional target for broadly treating retinitis pigmentosa regardless of the genetic cause.

NIH R01HL143469 KC Kent, L-W Guo, S Gong (MPI) 07/01/18-06/30/22 $396,296 annual direct cost “Targeting PERK: An Endothelium-Protective Stent-Free Strategy for Mitigation of Intimal Hyperplasia After Vascular Surgery ” Role: MPI

Google Scholar All Since 2014

Citations 1315 970 h-index 21 18

i10-index 41 28

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Goal: Drug-eluting stents for treating flow-limiting vascular diseases cannot completely prevent vessel re-narrowing and, furthermore, generate blood clotting risks. Our preliminary studies identified a new drug target and a novel biomimetic nanoplatform for stent-free drug delivery. We will test the combination of these two, which may lead to stent-free treatment methods that benefit millions of patients with cardiovascular disease. NIH R01HL133665 Lian-Wang Guo (PI) 06/01/16- NCE to 05/31/21 $273,250 annual direct cost ”BET Bromodomain Proteins as Novel Targets for Prevention of Intimal Hyperplasia after Vascular Surgery” Role: PI Goal: This project aims to pin down the specific target underlying the inhibitory effect of a pan-BET inhibitor (JQ1) on smooth muscle cell pathogenesis/intimal hyperplasia (IH) that we observed, with a long term goal of developing better anti-IH therapeutics.

NIH R01HL129785 KC Kent, L-W Guo, S Gong (MPI) 07/01/16- NCE to 06/30/21 $336,230 annual direct cost “Development of unimolecular nanoparticle-mediated periadventitial drug delivery system for sustained and targeted inhibition of intimal hyperplasia following open vascular reconstruction ” Role: MPI Goal: This project aims to develop unimolecular micelle nanoparticle-mediated multifunctional drug delivery platform for treating intimal hyperplasia following open cardiovascular surgery. 1UH54HL119810-06 (1118-SUB) NIH Center for Accelerated Innovations-Cleveland Clinic (NCAI-CC) Award Kent/Guo (co-PIs)

Total direct (including cost share) $151,734 08/01/18 – 01/21/20 “Pericelle: a nanoparticle based perivascular drug delivery system to prevent stenosis and failure in vascular reconstruction surgeries.” Role: co-PI Goal: We will test a prototype product to prevent stenosis and failure in vascular reconstruction surgeries. Our product (Pericelle, all-inclusive kit) combines 1) a unimolecular micelle nanoparticle and 2) a “triblock” hydrogel, which together, facilitate localized and sustained delivery of the anti-restenotic drug.

The Ohio State University Accelerator grant Craig Kent, L-W Guo (Co-PIs) 01/01/18 – 12/31/20 (TECG20170069) Total $100,000 ”Perivascular Drug Delivery Nanoplatform for Preventing Restenosis After Open Surgery” Role: co-PI Goal: We will conduct large animal experiments to test the viability of a nanoparticle-based perivascular drug delivery system to prevent intimal hyperplasia following open vascular surgery. UH54HL119810-07/NCAI-19-12 NIH Center for Accelerated Innovations-Cleveland Clinic (NCAI-CC) Award Bowen Wang (PI)

Total direct (including cost share) $150,501 8/01/19 – 7/31/20 “NanoPlatyx-1, A Biomimetic Nanocluster Product for Stent-Free Management of Restenosis” Role: co-Investigator Goal: We aim to collect data on the biomimetic nanocluster product that we deem most vital for successful RFD meeting and IND submission.

AHA Postdoctoral Fellowship (20POST35210967) Mengxue Zhang (PI) 01/01/20 – 12/31/21 “Role of Epigenetic Reader BRD4 in Smooth Muscle Cell's Degenerative Phenotype Transition and Abdominal Aortic Aneurysm” Role: Mentor Goal: This project is expected to establish BRD4 blockade as an effective epigenetic intervention strategy to mitigate Smooth muscle cell’s degenerative phenotype transition and thereby stop or slow AAA progression.

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b) Proposals for Research Funding that are Pending NIH R01EY031325-01 Lian-Wang Guo (PI) 12/01/19-11/30/24 (Pending: Scored and to be resubmitted) “RPE cell self-protective mechanisms” Goal: We propose to study a protective role of the sigma-1 receptor in RPE pathophysiology and autophagy mechanisms. Role: PI

NIH R01 HL153867-01 Lian-Wang Guo (PI), Kent (MPI) 07/01/20-06/30/25 (Pending) “Epigenetic modulation for non-surgical aneurysm mitigation” Goal: To date, there is no medical therapy to stop or slow the disease progression toward deadly aorta rupture. We will investigate a newly discovered potent pathogenic factor in hope of that a viable therapy could eventually become available for this dangerous aorta disease. Role: PI NIH (S10) Shared Instrument Grant to purchase small animal PET-CT scanner Mitchell Stacy (PI) (Pending) “PET/CT imaging for quantitative assessment of lower extremity physiology in peripheral arterial disease” Goal: The proposed studies will be focused on quantitative PET/CT imaging in the setting of limb ischemia, with the goal of large animal and clinical translation of these methodologies. Role: co-Investigator c) Funded Research, including Contracts and Clinical Trials, on Which the Candidate is or has been the Co- Investigator NIH R01 HL068673 K Craig Kent (PI) 06/01/14-05/31/19 $250,000 annual direct cost (no cost extension) ”TGF-beta and Smad3 in intimal hyperplasia after vascular bypass” Role: co-Investigator; 5% effort. I coordinate experiments, personnel, finance, communication with and report to NIH. The ultimate goal of this proposal is to identify agents that inhibit intimal hyperplasia and enhance adaptive remodeling, thus effectively preventing the development of recurrent disease following vascular interventions, a process associated with substantial cost, morbidity and mortality.

d) Funded Training Grants on Which the Candidate is or has been the Equivalent of the Principal Investigator None e) Proposals for Training Grants that are Pending or were Submitted but not Funded NIH T32 postdoctoral training grant renewal (DHLRI/Nationwide Children’s Hospital joint, for cardiovascular research) (Pending) Douglas Lewandowski (PI)

Role: Postdoc mentor

f) Any other Funding Received for the Candidate’s Academic work Completed grants: Wisconsin Alumni Research Foundation (WARF) Accelerator program L-W Guo (PI) 08/01/16 – 07/31/18 Total $150,000 ”A porcine trial of non-constrictive polymer sheaths as perivascular drug delivery platforms suitable for treating restenosis after open surgery” We will test a perivascular drug delivery polymer platform in pigs for safety and efficacy evaluation in preparation for clinical trial application. NIH R01EY022678 Lian-Wang Guo (PI) 09/01/12-07/31/17

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$220,500 annual direct cost ”Sigma-1 Chaperone-Mediated in vivo Neuroprotection in the Retina” Goal: This project investigates the protective role of the sigma-1 receptor in retinal degenerative diseases with a goal of identifying new therapeutic targets for prevention of blindness. Role: PI State of Wisconsin (ID 2832) Lian-Wang Guo (PI) 09/01/14-08/31/16 Wisconsin Partnership Program New Investigator Award $100,000 total direct cost “Nanoparticles for Treating Restenosis: Sustained and Targeted Local Drug Delivery” Goal: We will use nanoparticles as drug carriers and rapamycin as a model drug to develop perivascular drug delivery methods. Role: PI American Heart Association (14GRNT20380854) K. Craig Kent (PI) 07/01/14-06/30/16 ”Screening for Selective Inhibitors of Restenosis” $143,000 total direct cost Goal: The goal is to identify lead compounds for a new generation of anti-restenosis drugs that preferentially inhibit smooth muscle cells versus endothelial cells. Role: Co-Investigator UW-Madison Lian-Wang Guo (PI) 11/01/14-10/31/15 Morgridge Institute for Research and the James Christenson Estate Macular Degeneration Research Award (UDDS 531800) $25,000 total direct cost “Assessment of the Sigma-1 receptor as a therapeutic target for protection against retinal pigment epithelial cell degeneration” Goal: The focus of this project is to assess the sigma-1 receptor as a therapeutic target for treatment of age-related macular degeneration. Role: PI

UW-Madison ICTR K. Craig Kent (PI) 07/01/13-05/31/14 University of Wisconsin Institute for Clinical and Translational Research (ICTR) Pilot Award $50,000 (total) “HTS ON SMOOTH MUSCLE vs ENDOTHELIAL CELLS FOR ANTI-RESTENOSIS DRUGS” Goal: This project aims to use a high throughput screening approach to discover compounds that mitigate smooth muscle cell growth while preserving the viability of endothelial cells. Role: Co-Investigator

UW McPherson Eye Institute Lian-Wang Guo (PI) 07/01/09-06/30/10 UW Eye Research Institute Rapid Response Initiative Grant $30,000 (total direct cost) “Protection of Retinal Neurodegeneration by the Sigma-1 Receptor Chaperone” Goal: We aimed to assess the retinal protective role of the Sigma-1 receptor in knockout mice. Role: PI

NIH R0GM033138 Arnold E. Ruoho (PI) 05/03/07-04/30/11 $1647,070 (total 5-year direct cost) “STRUCTURE AND REGULATION OF GPCR-G PROTEIN INTERACTIONS IN THE VISUAL SYSTEM” Goal: The focus of this project is to elucidate the mechanisms of phosphodiesterase 6 functional regulations in the visual signal transduction. Role: Co-Investigator 6) List of Prizes and Awards for Research, Scholarly or Creative Work 2018 NIH Center for Accelerated Innovations-Cleveland Clinic (NCAI-CC) Award 2017 The Ohio State University Accelerator (translational research) Award 2016 Wisconsin Alumni Research Foundation (WARF) Accelerator Award 2014 Morgridge Institute for Research and the James Christenson Estate Macular Degeneration Research Award 2014 Wisconsin Partnership Program New Investigator Award (4 out of 20 applicants) 2008 The UW Eye Research Institute Rapid Response Initiative Award 2003 Award of Shanghai Science & Technology Progress 1996 Award of Excellent Scientific and Technological Articles of Young Researchers, Chinese Society of Plant

Physiology 1994 Outstanding Graduate Student Scholarship, Shanghai Institute of Plant Physiology, Chinese Academy of

Sciences

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TEACHING 1) Undergraduate, graduate, and professional courses taught

Period offered

Course Number and Title (Credit hours)

Enroll-ment

% Taught, Role Student Eval.

Peer Eval.

Other Eval.

Instr. Method

2019 Spring Semester

Physiology 6102, GI Physiology, Class#1486. Total 8 lectures and one exam.

37, mostly graduates, and senior undergrads

>80%, I taught 8 of total 10 GI Physiology lectures, plus final exam

Yes. SEI report

No No Re-designed lectures and exam questions

2019 Spring Fall 2018 Spring Fall

BSGP8999-7018, Research in Integrated Biomedical Science

Biomedical sciences Graduates

5% Average class size 15

No No No PhD program trainer

2019 Summer Fall 2018 Spring Fall

BSGP7930, Individual Studies in Integrated Biomedical Science

BSGP student

1 graduate student

No No No PhD program trainer

4/16/2018 Grand round, Vascular Division, OSU Dept of Surgery

Residents and fellows

30 min, one time. Topic: Epigenetic regulators in vascular disease

No No No ppt slides

8/2/2017 Dept Physiology PhD students meeting

12 PhD students

60 min, one time. Topic: Sigma receptors

No No No ppt slides

7/14/2017 Meet the Faculty Video Recording

30 PhD candidate students

15 min, one time. Topic: Retinal degeneration and protection

No No No ppt slides

2) Involvement in graduate/professional exams, theses, and dissertations, and undergraduate research 10/2014-5/2019 Committee member (dissertation, candidacy exam, thesis defense) for my PhD student Mengxue

Zhang, Department of Surgery, University of Wisconsin-Madison and then The Ohio State University, OH.

07/2017 Graduate Faculty Representative for the dissertation defense of Eric Danhart, Mark Foster lab,

Department of Chemistry and Biochemistry, The Ohio State University, OH. 2015-17 Committee member, for graduate student Mirnal Chaudhary, Department of Surgery, University of

Wisconsin SMPH, Madison, WI 2015-17 Committee member, for PhD Student Nathan York, Department of Pediatrics, University of

Wisconsin SMPH, Madison, WI 2014-17 Committee member (candidacy exam and thesis defense), for PhD Student Guojun Chen,

Department of Biomedical Engineering, University of Wisconsin College of Engineering, Madison, WI

2013 Committee member, for PhD student thesis defense, Akihiro Ikeda Lab, Department of Medical

Genetics, University of Wisconsin SMPH, Madison, WI

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a) Graduate Students: Number Completed and Number Current My PhD student Mengxue Zhang just graduated (thesis defense completed on 5/3/2019). Another student, Yitao Huang, has been enrolled (the PhD program started on 5/8/2019). I co-supervised (with Dr. Craig Kent) two doctoral students (dissertation, candidacy exam, and thesis defense) and one master’s student (thesis). b) Describe any noteworthy accomplishments of graduate students for whom you have been the advisor of record, for example, publications during or emanating from graduate program, awards for graduate work, prestigious post-docs or first post-graduate positions. 04/2019 I mentored my then PhD student Mengxue Zhang to win a Young Investigator Award (YIA); she was

invited to give an oral presentation at the Metabolic Cardiovascular Disease Symposium (satellite to ATVB, the premier vascular biology conference), Boston, MA. May 13, 2019.

11/2017-3/2019 Yitao Huang (BSc) initially joined my lab as staff Research Assistant. Under my mentorship in the

past two years, Yitao has published two co-authored papers with another first-author paper under review. He was lately admitted to the OSU Biomedical Sciences Graduate Program for PhD study with me.

05/2017 I mentored PhD student Mengxue Zhang to win the American Heart Association (AHA) Predoctoral

Research Fellowship award that has covered her two-year stipend. 06/2016 I co-mentored Bowen Wang to win the American Heart Association Predoctoral Research Fellowship. Both PhD students (Mengxue Zhang and Bowen Wang) published first-author and co-authored

papers before graduation. 05/2015 I mentored Mirnal Chaudhary, a graduate student, to win the 2015 Vascualr Research Initiatives

Conference (VRIC) travel award. 04/2014 I co-mentored Sarah Franco, PhD student in Craig Kent’s lab, to win the SACNAS Conference Best

Presentations Award. c) Undergraduate Research Mentoring Olivia Pollack, a high school student, gained voluntary research experience under my mentorship in the summers of 2018 and 2019. Luke Collin, an undergraduate, is doing research in my lab. I have mentored 10 undergraduate students who did voluntary research in my lab, 6 of them went to medical school. One of these students, Yifan Zhou, obtained a paid research position at NIH after leaving my lab. He is now enrolled in an MD/PhD program provided by NIH and Washington University. Outside of my lab, I mentored Emily Anaya in a Chancellor’s Scholarship Mentor Friend Program (2014-2017). d) Describe any noteworthy accomplishments of undergraduate students, in particular related to research, for whom you have been the advisor of record (publications, posters, honors or student awards). 10/2017-5/2019 I mentored Corey Williams for research in my lab. He has been admitted to an OSU medical student

program. 01/2017 I mentored Annie Yao to win Comprehensive Honors on graduation from University of Wisconsin-

Madison. She is now a medical student of the DO School of Des Moines University, Iowa. 04/2015 I mentored Chris J. Little to win the 2015 Shapiro Summer Research Award. Chris was then admitted

to the University of Wisconsin-Madison medical school, and he is now a resident in the University of Washington Medical School.

04/2015 I mentored Annie Yao to win the 2015-2016 Sophomore Research Fellowship (15 out of 67

applicants). 04/2014 I mentored Annie Yao to win the University of Wisconsin-Madison Welton Summer Honors

Apprenticeship Award. Four undergraduate student researchers (Little, Zent, Zhou, Yao) earned co-authorship on my

papers. In particular, Chris Little is a co-first author in my recent publication (JACC BTS 2018).

3) Involvement with Postdoctoral Scholars and Researchers

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2/2017-present I am mentoring 4 postdoctoral researchers, Drs. Jing Li, Hongtao Shen, Qingwei Wang, and Mengxue Zhang (my former PhD student). I am also co-mentoring Dr. Go Urabe and Dr. Takuro Shirasu (jointly appointed in my and Dr. Kent’s labs).

05/2017 My postdoc mentee Huan Yang’s work was selected for oral presentation at the Association for

Research in Vision and Ophthalmology (ARVO) meeting. 05/2017 I mentored Lei Zhao to win the Association for Research in Vision and Ophthalmology (ARVO)

meeting travel award (declined because of the awardee‘s absence after my lab relocation). Before relocation to OSU, I mentored one scientist (Dr. Mavlyutov) and 3 postdocs. In addition, I have mentored 6 visiting scholars at the postdoctoral (PhD or MD) level. They all contributed to publications. In particular, Dr. Yichen Zhu’s one-year visiting research resulted in a first-author paper in Scientific Reports; Dr. Jun Li published a first-author paper in Molecular Vision; Dr. Yingmei Fu is co-first author in a paper in Molecular Neurodegeneration. All of these were achieved in a one-year period of visiting research. In addition, I trained medical professionals into capable researchers. While working closely with Shakti Goel, MBBS, his hard work resulted in 3 first-author papers. Both Dr. Toshio Takayama and Dr. Go Urabe are vascular surgeons. They came from University of Tokyo with little research experience. By now they have both published first-author papers and multiple co-authored papers in my lab collaborating with Dr. Kent’s lab. 4) Extension and Continuing Education Instruction 09/28/2018 Research Retreat, Division of Vascular Disease and Surgery, Dept of Surgery, The Ohio State

University, presentation to residents, fellow, and surgeons. 08/28/2018 Research Showcase, Dept of Surgery, The Ohio State University, presentation to PhD students,

postdoc researchers, residents, and faculty. 03/16-01/17 Director, Research in Progress Seminar Series, Department of Surgery, University of Wisconsin-

Madison. Duties include planning for the academic year, inviting and introducing speakers, and supervising seminars. As the director I enhanced teaching by designating a student (especially undergrads) to introduce the speaker so that students can practice public speaking. I also gave students or junior researchers privileged time to ask questions after the talk.

03/ 2016 Lecture to the McPherson Eye Research Institute— The Sigma-1 Receptor, a Secret Weapon for Fighting Blindness. University of Wisconsin, Madison, WI. Faculty evaluations available

10/15-12/16 Director/lecturer of the Basic Science Curriculum for residents and fellows in the Deaprtment of

Surgery, University of Wisconsin, Madison, WI. Provided 5 lectures (student evaluations available). 02/18/ 2015 Guest Lecturer— Nanoparticles for treating Restenosis, for the Cellular and Molecular Pathology

PhD Program. University of Wisconsin, Madison, WI. Student evaluations available 03/ 2014 One lecture to general public audience— The Sigma-1 Receptor and Its Protection for the Retina.

University of Wisconsin-Madison, WI 02/ 2013 Guest Lecturer— High Throughput Screening and Identification of Selective Inhibitors of

Restenosis, for residents of Department of Surgery, University of Wisconsin, Madison, WI 08/ 2011 Guest Lecturer— RGS proteins, for the PhD students in the Molecular Cellular Pharmacology

Program. University of Wisconsin, Madison, WI 08/ 2009 Guest Lecturer— Phosphodiesterase 6, for the PhD students in the Molecular Cellular

Pharmacology Program. University of Wisconsin, Madison, WI

5) Curriculum Development This 2019 Spring semester was my first time of class teaching at OSU (Physiology 6102, Class#1486). Since the last-year instructor left OSU, I composed (de novo) the slides for all of the 8 lectures of gastrointestinal (GI) Physiology I taught (Dr. Khan gave 2 lectures) and redesigned the exam. The new teaching materials I put together are focused, concise, and coherent, and relatively easy for the students to understand. Where applicable, at any appropriate moment I pulled out earlier contents I taught in order to logically connect with the new content so that the students could have an integrated view of GI physiology. In addition, I used many questions to challenge the students to measure their levels of comprehension while using these questions as opportunities to clarify basic concepts.

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6) Brief Description of your approach to and goals in teaching, major accomplishments, plans for the future in teaching Class teaching philosophy: There can be two ways to teach. First, the teacher dumps information of 100 (arbitrary unit) to the students, and they can only comprehend 20% of it. Second, the teacher carefully selects information of 50 for the students and they can efficiently absorb 40%. I prefer the latter. I used this philosophy in my teaching in the Physiology 6102 class, and the impact was evident. The gastrointestinal (GI) physiology used to be under-appreciated and always scheduled at the end of the Physiology 6102 courses. However, using my teaching method, I found that students’ interest was ignited. Many of them voluntarily answered my questions, and their answers were mostly correct. This encouraging class atmosphere further fueled their enthusiasm, as evidenced by that some students started to proactively ask in-depth questions, or stayed after class to discuss with me. Three students emailed me for questions. For future teaching, I am scheduled for the same lectures in the Spring semester of 2020 (Physiology 6102, Class#1486). Researcher teaching philosophy: I have a clear goal: I want the research activity of my postdocs and PhD students to be fun-based rather than merely labor-based. I believe this is the best way for them to re-discover their creativity. To apply this philosophy, I have judged their productivity more by creative thinking than data volume. I tried to instill this mantra at lab meetings and in-person conversations. I also passed my sentiment to the enrolled and candidate PhD students at my talks. When a postdoctoral researcher (Huan Yang) left my lab, he said he would miss my teaching style and the freedom in my lab the most. 7) Evaluation of Teaching I used the enthusiasm levels of students in response to my spontaneous questions as a measure of their interest and comprehension. I sensed elevated interest based on increased questions from the students. To further enhance their attention, I chatted with some students before the class to remember their names, and encouraged them to answer questions by calling their names. I “begged” for their feedback or suggestions and also listened to their concerns and anxiety about the exam or exam question formats etc. Through these channels of communication, I could most accurately evaluate the impact of my innovations in teaching and the areas to improve. SEI (student evaluation of instruction) Report: I was ranked at “Excellent” (highest) by 62% student evaluators, at “Good” and “Neutral” by 23% and 15% evaluators, respectively. For instructor evaluation, I was scored at 4.46 (5 is the highest), above the OSU university wide average (4.37/5). 8) Awards and Formal Recognition for Teaching None 9) Other Academic Advising None 10) Teaching Professional Development I learned how to use animation and video tools at Lunch and Learn for Instructors presented by a tutor from McGraw-Hill, the publisher of Human Physiology, 3/1/2019 at OSU. I have attended two Teaching Seminars in 2019 (4 more scheduled), where Dr. Beth Lee, Associate Professor in Department of Physiology & Cell Biology at OSU teaches various techniques for efficient teaching. I will use these new skills to improve my teaching.

SERVICE 1) List of Editorships or Service as an Editorial Reviewer or Board Member for Journals, University Presses, or Other Learned Publications 2019 Reviewer, Biomaterials (ISSN: 0142-9612) (IF: 10.1) 2019 Reviewer, IOVS (Investigative Ophthalmology & Visual Science)(ISSN 0146-0404) 2019 Reviewer, Medical Science Monitor (ISSN: 1643-3750) 2019 Reviewer, Theranostics (ISSN: 1838-7640) (IF: 8.5) 2019 Reviewer, Frontiers in Neuroscience (ISSN: 1662-453x) 2019 Reviewer, Life Sciences (ISSN: 0024-3205) 2019 Reviewer, Theranostics (ISSN: 1838-7640) (IF: 8.5)

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2018 Reviewer, Arteriosclerosis, Thrombosis, and Vascular Biology (ATVB) (ISSN 1079-5402) (IF: 6) 2018 Reviewer, ACS Biomedical Engineering (ISSN 2373-9878) 2018 Reviewer, Progress in Retinal and Eye Research (ISSN1350-9462, Impact factor: 11.5) 2018 Reviewer, Scientific Reports (ISSN 2045-2322) 2017 Reviewer, Scientific Reports (ISSN 2045-2322) 2017 Reviewer, IOVS (Investigative Ophthalmology & Visual Science)(ISSN 0146-0404) 2016 Reviewer, Investigative Ophthalmology &Vision Science (ISSN 0146-0404) 2016 Reviewer, Circulation Research (ISSN 0009-7330) (IF: 15) 2016 Reviewer, Cellular Physiology and Biochemistry (ISSN 1015-8987) 2016 Reviewer, Scientific Reports (ISSN 2045-2322) 2015 Reviewer, Biochemical Journal (ISSN 0264-6021) 2015 Reviewer, Scientific Reports (ISSN 2045-2322) 2014 Reviewer, Archives of Biochemistry and Biophysics (ISSN 0003-9861) 2014 Reviewer, Arteriosclerosis, Thrombosis, and Vascular Biology (ATVB) (ISSN 1079-5402) (IF: 6) 2014 Reviewer, Journal of Cellular Physiology (ISSN 1097-4652) 2014 Reviewer, Nature Communications (ISSN 2041-1723) (IF: 12) 2014 Reviewer, Stem Cell Research (ISSN 2041-1723) 2014 Reviewer, Molecules (ISSN 1420-3049) 2012 Reviewer, Molecular Vision (ISSN 1090-0535) 2012 Reviewer, American Journal of Pathology (ISSN 0002-9440) 2011 Reviewer, Nature Molecule Pages 2011 Reviewer, Molecular Vision (ISSN 1090-0535) 2011 Reviewer, BMC Medical Genetics (ISSN 1471-2350) 2010 Reviewer, Biochemistry (American Chemical Society) 2010 Reviewer, Nature Molecule Pages 2009 Reviewer, Process Biochemistry (ISSN: 1359-5113)

2) List of Offices Held and Other Service to Professional Societies. List of Organization in Which Office was Held or Service Performed. Describe Nature of Organization (Open or Elected Membership, Honorary) None 3) List of Consultation Activity

None

4) Clinical Services State Specific Clinical Assignments None

5) Other Professional/Public Community Service directly related to the Candidate’s Processional Expertise, if not Listed Elsewhere 7/2019 NIH Study section: NIH Fellowship Review Panel: Cell Biology, Developmental Biology, and

Bioengineering F05-D(21). Review Panel meeting took place on July 16-17, 2019 in Bethesda, MD. 6/2018 NIH Special Emphasis Review Panel/Study Section for Fellowships:

Cell Biology, Developmental Biology, and Bioengineering (ZRG1 F05U). This service inluded reviewing and scoring 12 grant applications (I spent two weeks to finish), and a 2-day study section meeting at NIH in Bethesda.

5/2018 Reviewer, pilot grant at the Institute for Clinical and Translational Science (ICTS), University of

California, Irvine (UCI), one of the hubs--The Clinical and Translational Science Awards (CTSA) Program of the NIH National Center for Advancing Translational Sciences (NCATS). It supports a national network of medical research institutions (called hubs). Manager: April Bagaporo.

I spent 2-3 days to review and score grant applications and write critiques. 2/2018 Reviewer, University of Wisconsin-Madison Institute of Clinical and Translational Research Pilot

Grants. I reviewed and scored grant application and wrote NIH-stype critiques (in 2-3 days). 9/2017 Reviewer, the Ohio State University College of Medicine Dean’s Discovery Grant Program. I reviewed and scored grant application and wrote NIH-stype critiques (in 2-3 days).

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5/2017 Reviewer, University of Wisconsin-Madison Institute of Clinical and Translational Research Pilot Grants.

I reviewed and scored grant application and wrote NIH-stype critiques (in 2-3 days). 2014-16 Research grant Review Committee (review Start-up, Pilot, or Bridge grants three rounds per year,

attend committee meetings and vote to score grants mimicking NIH study section). Department of Surgery, University of Wisconsin-Madison, WI. (I reviewed total 11 grants).

2013 Reviewer, University of Wisconsin Institute of Clinical and Translational Research Pilot Grants. 2013 Reviewer, French National Agency for Research (equivalent of NIH in the US). I reviewed and scored grant application and wrote critiques (in 3 days). 2009 Reviewer, the UW-Madison Eye Research Institute Rapid Response Initiative Grants.

6) Administrative Service a) Unit Committees 2017-present Faculty candidate interviewer. There was active faculty recruitment in the past two years in the

Dept of Physiology & Cell Biology and Dept of Surgery. I interviewed over a dozen of candidates by reading their application materials, talking in person, attending presentations and chalk talks, and writing evaluations.

3/2014-1/2017 Member of the Research Committee, University of Wisconsin Dept of Surgery, UW-Madison, WI

b) College or University Committees 7/2019-present Committee member for Dr. Mingtao Zhao, new faculty member in the Center for Cardiovascular

Research at Nationwide Children's Hospital, Columbus. I was invited by Dr. Vidu Garg, the center’s director.

3/2019-present Committee member for the search of Vice Chair for Research (Dept of Urology), The Ohio State

University. The committee is led by Dr. Cheryl Lee, Chair of Dept of Urology. I participated in the discussion (via email) to finalize the position description, and the committee meeting (2 hours) to select 4 candidates for interview, and a whole-day interview session.

4/2017-present Committee member for faculty search (Dept of Ophthalmology, chaired by Dr. Colleen Cebulla),

The Ohio State University. I have participated in total 5 committee meetings (1.5 -2 hours each time) and interviewed 6 candidates (45 min each time). Two of the candidates I recommended ranked at number 1 and number 2. The negotiation with the number 1 candidate is ongoing for recruitment.

9/2014-1/2017 University of Wisconsin Faculty Senator, representative of the Dept of Surgery, UW-Madison, WI 3/2015-1/2017 William S. Middleton Memorial Veterans Hospital Research Grant Review Committee (review grants

for VA investigators and provide critiques), Madison, WI

c) Initiatives Undertaken to Enhance Diversity in your Unit, College or the University None d) Administrative Positions Held, e.g. Graduate Studies Chair None e) Service as a Graduate Faculty Representative on a Dissertation in another Unit or University None 7) Advisor to Student Groups and Organizations None 8) Office of Student Life Committees a) List Office of Student Life Committees on which You have served None

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b) Summarize participation in Student Life programs such as fireside discussions, lectures to student groups outside of your unit, addresses or participation at student orientations I regularly participated in student interview, orientation, and scientific retreat events of the MCDB (Molecular Cellular and Development Biology) and BSGP (Biomedical Sciences Graduate Program) PhD programs. 9) List of prizes and awards for service to your profession, the university or your unit None 10) Brief elaboration that provides additional information about service activities listed above. In addition to the service activities listed above, I had meetings with >10 outside speakers during their visit to OSU. I attended almost all of the Dept of Physiology faculty meetings and several relevant Dept of Surgery faculty meetings, monthly research meetings of the Division of Vascular Surgery and Disease, Dept of Surgery Research Showcase seminars. I also served as poster judges at Research Day events. In the past few years, I have mentored Bowen Wang in his professional development. I identified outstanding talents and qualities in Bowen as a researcher and invested enormous time in scientific discussions with him on a daily basis. I have been instructing his project development and manuscript writing and grant applications. To my great pleasure, Bowen has quickly grown in academia and risen to the rank of research faculty. I was invited by Dr. Benjamin Segal (co-director of the OSU Neurological Institute) to be a mentor for Dr. Yousef Hannawi, MD, Assistant Professor, Department of Neurology. I have met with Dr. Hannawi and planned for more meetings.

Mentee Awards

12/2019 Mentored Dr. Mengxue Zhang to win the American Heart Association (AHA) Postdoc Fellowship Award. 05/2019 Mentored Mengxue Zhang to win the Young Investigator Award at the Metabolic Cardiovascular Disease

Symposium (satellite to ATVB), Boston, MA on May 13, 2019. 05/2017 Mentored Mengxue Zhang to win the American Heart Association (AHA) Predoc Research Award. 05/2017 Mentee Annie Yao won Comprehensive Honors on graduation from University of Wisconsin-Madison, she

is now admitted to the DO School of Des Moines University, Iowa. 04/2017 Mentored Lei Zhao to win the Association for Research in Vision and Ophthalmology (ARVO) meeting travel

award (declined because of the absence of the awardee). 06/2016 Co-Mentored Bowen Wang to win the American Heart Association (AHA) Predoc Research Award. 05/2015 Co-Mentored Mirnal Chaudhary, postdoc, to win the 2015 Vascualr Research Initiatives Conference (VRIC)

travel award. 04/2015 Mentored Chris J. Little, medical student, to win the 2015 Shapiro Summer Research Award. 04/2015 Mentored Annie Yao to win the 2015-2016 Sophomore Research Fellowship (15 out of 67 applicants). 04/2014 Mentored Annie Yao to win the UW Welton Summer Sophomore Honors Apprenticeship award. 04/2014 Co-Mentored Sarah Franco, PhD student to win the SACNAS Conference Best Presentations Award.

Miscellaneous

Apr 2011 Crazy Legs Runner, fundraising for UW Athletics Department, Madison, WI May 2013 Volunteer at Brat Fest, fundraising for Wisconsin Youth Symphony Ochestra, Madison, WI Aug 2013 Half-Marathon runner, fundraising for "Healthy Women, Healthy Babies" (parternered with the UW Department of Obstetrics and Gynecology) Madison, WI Nov 2013 Volunteer at Madison Marathon, fundraising for Hamilton Middle School Science Olympiad Program, Madison, WI May 2014 Volunteer at Brat Fest, fundraising for Wisconsin Youth Symphony Ochestra, Madison, WI Nov 2014 Volunteer at Madison Marathon, fundraising for Hamilton Middle School Science Olympiad Program, Madison, WI May 2015 Volunteer at Brat Fest, fundraising for Wisconsin Youth Symphony Ochestra, Madison, WI Nov 2017 Attended Controversies in Vascular Diseases, the 10th Annual Vascular Non-Invasive Testing Symposium on Friday November 3rd and Saturday, November 4th Nationwide & Ohio Farm Bureau 4-H Center, Bob Evans Memorial Auditorium, Columbus OH 43210. Jan 2018 Attended MCDB PhD program graduate recruitment session. OSU, Columbus OH. Sept 2018 Attened PCB graduate recruitment session. OSU, Columbus OH. Dec 2018 Attended Biomedical Sciences Graduate Program Annual Retreat. OSU, Columbus OH. Jan 2019 Attended PCB chair search meetings and evaluation. OSU, Columbus OH. July 2019 Runner, Ohio State Ross Heart Hospital TriFit Challenge.