Upload
amber-petty
View
31
Download
1
Embed Size (px)
DESCRIPTION
Learning Gene Therapy through a Bacterial Transformation Lab. Kristen Kopf Elk Grove H.S. RET 2010 Teaching Module University of Illinois at Chicago December 4, 2010. Inspiration for Unit. Summer research included techniques used for gene therapy Bacterial Transformation - PowerPoint PPT Presentation
Citation preview
KRISTEN KOPFELK GROVE H.S.
RET 2010 TEACHING MODULEUNIVERSITY OF ILLINOIS AT CHICAGO
DECEMBER 4 , 2010
Learning Gene Therapy through a Bacterial Transformation Lab
Inspiration for Unit
Summer research included techniques used for gene therapy
Bacterial Transformation
Techniques are possible in a high school labUnit reflects current and exciting prospects
in science technology
Target Student Population
Students in 9th and 10th grades taking BiologyAverage students
Current ACT score 16-19 6th grade – 9th grade reading levels
Includes at-risk and English-language learners
Students have already learned about DNA and protein synthesis
Unit Objectives
Describe how we can change an organism’s traits
Explain the uses for and limitations of gene therapy
Explain the purpose of the laboratory steps for gene therapy
Describe what is meant by the term “transformed organism”
Read and follow a lab procedureIdentify a control in an experiment
Activities
Gene Therapy WebquestUnit Overview PictureBacterial Transformation PowerPointBacterial Transformation IntroductionBacterial Transformation LabLab Quiz
Gene Therapy Webquest
Webquest created by the University of Utah
Students will learn… Definition of gene therapy Compare and contrast vectors used in gene therapy Practice reading for information
Unit Overview Picture
Illustrates the major steps necessary for gene therapy
How do we isolate the gene? How do we get the gene into the cell? How do we get the cell to express the protein?
For practice, cut up the stages and have students place them in order
Bacterial Transformation PowerPoint
Goes more in depth into the laboratory steps necessary for gene therapy
Restriction Enzymes PCR Gel Electrophoresis Transform Bacteria Induce protein expression
Students take Cornell style notes
Lab Introduction
Students complete a reading and questions to discover:
Definition of transformed organisms Purpose of an experimental control How we can give organisms a new phenotype How we will induce the expression of the pBlu gene to
turn the bacteria from white to blue
Bacterial Transformation Lab
Cost = $109 for a kit with 8 stations Carolina Biological
Students transform bacteria to change their appearance from white to blue
Focus is on following a lab procedure Storyboard
Lab Quiz
Focuses on why students completed certain steps in the lab procedure
Example: Heat shock
Students must analyze plates and determine the control
Anticipated Outcomes
Increase in student engagement “Feel like real scientists”
Connection to past conceptsReinforcement of lab technique and data
analysis
Acknowledgements
RET 2010 Program NSF Grant #CBET EEC-0743068, NIH R01 NS055095, and R03 EY014357 (RAG).
The summer research was conducted in a facility constructed with support from Research Facilities Improvement Program Grant Number C06 RR15482 from the National Center for Research Resources, NIH.
Dr. Richard Gemeinhart, Research MentorDr. Andreas Linninger, Program DirectorStaff at Elk Grove High SchoolUniversity of Utah for the gene therapy webquest