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LAMPIRAN

Lampiran 1. Hasil Analisis Statistik

Descriptives

Kelompok Perlakuan Statistic

Std. Error

Hasil Emulgel Minyak Jintan Hitam Konsentrasi 5%

Mean 18.500 .5505 95% Confidence Interval for Mean

Lower Bound 16.972

Upper Bound 20.028

5% Trimmed Mean 18.517 Median 18.900 Variance 1.515 Std. Deviation 1.2309 Minimum 17.0 Maximum 19.7 Range 2.7 Interquartile Range 2.4 Skewness -.428 .913 Kurtosis -2.758 2.000

Emulgel Minyak Jintan Hitam Konsentrasi 7%

Mean 23.560 2.2069 95% Confidence Interval for Mean

Lower Bound

17.433

Upper Bound 29.687

5% Trimmed Mean 23.722 Median 24.400 Variance 24.353 Std. Deviation 4.9349 Minimum 15.4 Maximum 28.8 Range 13.4 Interquartile Range 7.2 Skewness -1.370 .913

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Kurtosis 2.947 2.000 Emulgel Minyak Jintan Hitam Konsentrasi 10%

Mean 25.900 1.8431 95% Confidence Interval for Mean

Lower Bound 20.783

Upper Bound 31.017

5% Trimmed Mean 25.883 Median 26.200 Variance 16.985 Std. Deviation 4.1213 Minimum 20.9 Maximum 31.2 Range 10.3 Interquartile Range 7.9 Skewness .064 .913 Kurtosis -1.378 2.000

a. Hasil is constant when Kelompok Perlakuan = Kontrol Negatif. It has been omitted.

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Tests of Normality

Kelompok Perlakuan

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig. Hasil 5% .227 5 .200* .874 5 .283

7% .344 5 .054 .859 5 .225 10% .167 5 .200* .977 5 .921

*. This is a lower bound of the true significance. a. Lilliefors Significance Correction c. Hasil is constant when Kelompok Perlakuan = Kontrol Negatif. It has been omitted.

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig.

Hasil Based on Mean 1.563 2 12 .249 Based on Median 1.053 2 12 .379 Based on Median and with adjusted df

1.053 2 6.688 .400

Based on trimmed mean 1.426 2 12 .278

a. Hasil is constant when Kelompok Perlakuan = Kontrol Negatif. It has been omitted.

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ANOVA Hasil

Sum of Squares df

Mean Square F Sig.

Between Groups 3222.106 4 805.527 93.987 .000 Within Groups 171.412 20 8.571 Total 3393.518 24

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Multiple Comparisons Dependent Variable: Hasil Tukey HSD

(I) Kelompok Perlakuan

(J) Kelompok Perlakuan

Mean Difference

(I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound

Upper Bound

5% 7% -5.0600 1.8516 .084 -10.601 .481 10% -7.4000* 1.8516 .006 -12.941 -1.859 Kontrol Negatif 18.5000* 1.8516 .000 12.959 24.041

7% 5% 5.0600 1.8516 .084 -.481 10.601 10% -2.3400 1.8516 .715 -7.881 3.201 Kontrol Negatif 23.5600* 1.8516 .000 18.019 29.101

10% 5% 7.4000* 1.8516 .006 1.859 12.941 7% 2.3400 1.8516 .715 -3.201 7.881 Kontrol Negatif 25.9000* 1.8516 .000 20.359 31.441 5% -18.5000* 1.8516 .000 -24.041 -12.959

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Post Hoc Tests

Lampiran 2. Dokumentasi Penelitian

Hasil Uji Saponin Hasil Uji Tanin

Kontrol Negatif

7% -23.5600* 1.8516 .000 -29.101 -18.019 10% -25.9000* 1.8516 .000 -31.441 -20.359

*. The mean difference is significant at the 0.05 level.

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Penambahan Minyak Jintan Hitam Penambahan Tween 10

Uji Homogenitas Uji Viskositas

Uji pH

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Uji Daya Sebar

Uji Daya Lekat

Aquadestilata (Kontrol Negatif)

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Emulgel Minyak Jintan Hitam Cotton Swab

Biakan Murni Bakteri Bio Safety Cabinet (BSC) Streptococcus mutans

Mikropipet Larutan standar McFarland 1 (setara dengan 3x108 CFU/mL jumlah bakteri)

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Pembuatan Larutan Bakteri Penyamaan Kekeruhan Larutan Bakteri Dengan Larutan Standar McFarland 1

Pengusapan Larutan Bakteri ke Media Muller Hinton Agar

Pencampuran Cakram ke Bahan Uji

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Cakram Dimasukkan ke Media Inkubasi di Inkubator Muller Hinton Agar

Hasil Zona Hambat di Media Muller Hinton Agar Setelah Inkubasi

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Lampiran 3. Ethical Clearance

Pengukuran Diameter Zona Hambat

Pencatatan Hasil Hasil Diameter Zona Pengukuran Hambat Kelompok Kontrol Negatif

Hasil Diameter Zona Hasil Diameter Zona Hasil Diameter Zona Hambat Kelompok Hambat Kelompok Hambat Kelompok Konsentrasi 5% Konsentrasi 7% Konsentrasi 10%

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Lampiran 4. Surat Izin Penelitian Laboratorium Farmasi FK Unissula

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Lampiran 5. Surat Izin Penelitian Laboratorium Mikrobiologi FK Unissula

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Lampiran 6. Laporan Hasil Evaluasi Sediaan Emulgel Minyak Jintan Hitam

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Lampiran 7. Surat Keterangan Hasil Penelitian Laboratorium Mikrobiologi FK Unissula

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Lampiran 8. Hasil Turnitin Karya Tulis Ilmiah

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Lampiran 9. Hasil Turnitin Naskah Publikasi

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