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Laboratory Diagnosis of Virus Infections

Laboratory Diagnosis of Virus Infections. Virus Diagnosis Direct demonstration of the virus, its antigens or nucleic acid Virus Isolation: – Chick embryo

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Page 1: Laboratory Diagnosis of Virus Infections. Virus Diagnosis Direct demonstration of the virus, its antigens or nucleic acid Virus Isolation: – Chick embryo

Laboratory Diagnosis of Virus Infections

Page 2: Laboratory Diagnosis of Virus Infections. Virus Diagnosis Direct demonstration of the virus, its antigens or nucleic acid Virus Isolation: – Chick embryo

Virus Diagnosis

• Direct demonstration of the virus, its antigens or nucleic acid

• Virus Isolation:– Chick embryo– Cell culture (cell line)– Animal inoculation

• Serology

Page 3: Laboratory Diagnosis of Virus Infections. Virus Diagnosis Direct demonstration of the virus, its antigens or nucleic acid Virus Isolation: – Chick embryo

Direct Demonstration of virus, antigens or nucleic acid

• Cytology (CPE: cell lysis, vacuolation, syncytia, changes in cell morphology, inclusion bodies)

• Electron microscope (number of virus particles)

• Genetic Probing using tagged probes specific for a unique target sequence in the viral genome

• Direct Immunofluorescence, ELISA, RIA, latex agglutination

Page 4: Laboratory Diagnosis of Virus Infections. Virus Diagnosis Direct demonstration of the virus, its antigens or nucleic acid Virus Isolation: – Chick embryo

Virus Isolation (cell line)

• Primary cell line

• Diploid cell line

• Continuous cell lines (HeLa)

Page 5: Laboratory Diagnosis of Virus Infections. Virus Diagnosis Direct demonstration of the virus, its antigens or nucleic acid Virus Isolation: – Chick embryo

Virus Isolation (cell line)

• Virus growth is detected in cell line by

CPE, interference, hemadsorption, hemagglutination)

Page 6: Laboratory Diagnosis of Virus Infections. Virus Diagnosis Direct demonstration of the virus, its antigens or nucleic acid Virus Isolation: – Chick embryo

Serology

• Detection of antibodies to the virus

• Has limitations

• IgM, 4-fold or more increase in IgG or high fixed titre

• Neutralization, hemagglutination inhibition, ELISA, indirect fluorescent test, RIA, latex agglutination