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Laboratory Diagnosis of Virus Infections
Virus Diagnosis
• Direct demonstration of the virus, its antigens or nucleic acid
• Virus Isolation:– Chick embryo– Cell culture (cell line)– Animal inoculation
• Serology
Direct Demonstration of virus, antigens or nucleic acid
• Cytology (CPE: cell lysis, vacuolation, syncytia, changes in cell morphology, inclusion bodies)
• Electron microscope (number of virus particles)
• Genetic Probing using tagged probes specific for a unique target sequence in the viral genome
• Direct Immunofluorescence, ELISA, RIA, latex agglutination
Virus Isolation (cell line)
• Primary cell line
• Diploid cell line
• Continuous cell lines (HeLa)
Virus Isolation (cell line)
• Virus growth is detected in cell line by
CPE, interference, hemadsorption, hemagglutination)
Serology
• Detection of antibodies to the virus
• Has limitations
• IgM, 4-fold or more increase in IgG or high fixed titre
• Neutralization, hemagglutination inhibition, ELISA, indirect fluorescent test, RIA, latex agglutination