Lab Safety

#1Do not bring food, drink, or tobacco into the lab.

Do not invite friends into the lab.Keep pencils, fingers, etc. out of your mouth, ears, eyes, nose, etc.

#2Wear protective clothing at all times.

Shoes are always requiredOpen-toed sandals are not allowed.

#3Do not take any cultures out of the lab for any reason.

#4Wipe the bench tops down with disinfectant both before you begin your work and after you have completed your work.

#5Keep nonessential books and clothing far away from your work area.

#6Wash your hands with soap and water for 15

seconds before and after you work in the lab, or if you leave the lab for any reason.

#7Do not mouth pipette ever!!!!!!!!!!

#8Be careful near bunsen burners.Tie long hair back away from the face.

Turn off burners when not in use.

#9Beware of the chemicals and reagents used in biochemical tests.

#10Report any accidents (spills, broken glass,

cuts, or injuries) to your instructor immediately.

#11Dispose of contaminated or used materials

quickly and in the manner set forth by your instructor.

#12Label all of your tubes and plates exactly as

directed by your instructor.Place all cultures for incubation exactly

where instructed or you risk their loss.Any cultures not handled accordingly will be

discarded!!!

#13Keep microscopes clean and the objectives free of oil.

Why is Lab Safety Important?Lab safety is a major aspect of every lab based

science class.Lab safety rules and symbols are needed so that

students do not injure themselves or their classmates.

Lab Safety Rules1. Conduct yourself in a responsible manner at

all times. No Horseplay!2. Do not touch any equipment, chemicals, or

other materials until told to do so. 3. Do not eat food, drink beverages, or chew

gum in the lab. Do not use lab glassware as food or beverage containers

4. Report ALL accidents immediately, even if you think it is minor.

ClothingWear:

• Goggles/safety glasses• Closed-toed shoes• Clothing made of

natural based fiber such as cotton

• Tie back long hair

Do NOT Wear:• Sandals• Jewelry• Loose or Baggy

clothing

Lab Safety EquipmentSafety Shower Safety GogglesEye Wash

Lab Safety EquipmentFire

Extinguisher Fire Blanket Fume Hood

Lab Safety SymbolsFound in your textbook

GlasswareHeatEye and FaceSharps

ElectricalAnimalChemicalFire

Other Chemical Hazards and Symbols

Flammable

Explosive

Toxic/Poison

Irritant

Corrosive

Environmental

Chemical Hazard Symbols and Definitions

Flammable – Any substance that will burn if exposed to an open flame.

Explosive – A substance that may explode if exposed to heat or flame.

Chemical Hazard Symbols and Definitions

Toxic/Poison – A substance that can lead to death if inhaled, ingested, or absorbed by the skin.

Corrosive – A substance that can destroy or burn living tissue and can eat away at other materials.

Chemical Hazard Symbols and Definitions

Irritant - A substance that causes inflammation upon contact with skin or mucous membranes.

Environmental - Substances that are harmful to the environment. They must be disposed of properly, not washed down the drain.

Accidents and InjuriesReport ALL accidents and injuries immediately!!! Be aware of safety hazards associated with

each chemical you use.Eye accident – Wash the eye with copious

amounts of water for at least 15 min.Fire Burns – Stop, Drop, and be covered with a

fire blanket or soaked with water. Do not remove clothing stuck to victim. Get medical attention immediately.