Step 1:Reagent & PCR

mixture preperation

Step 2: SARS-CoV-2 inactivation

Step 3:Virus RNA extraction

Step 4:PCR sample preparation

Step 5:QPCR processing

and analysis

LAB 1 LAB 3

LAB 2

Shaking Dry bath E-DB100S-PŸ 100°C Heating dry bathŸ 1.5/2ml and 15ml heating blockŸ Quantity: 1

Vortex Mixer E-VM-AŸ Continuous/Touch operationŸ Variable speed 0-2500rpmŸ Quantity: 2-3

Micro High Speed Centrifuge E-C15-12.2Ÿ 12 x 1.5/2ml Ÿ Max speed: 15000rpmŸ Plastic clip on rotor lidŸ Quantity: 1

Nano Centrifuge E-C7-Nano Ÿ Fixed Speed 7000rpmŸ Volume: 8 x 1.5/2ml tubes Ÿ Volume: 4 x 0.2ml PCR 8 StripsŸ Quantity: 1-2

Waste and Pipette Sterilization AutoclaveE-SA-300VF Ÿ 50 Liter Vertical AutoclaveŸ Sterilizing Temperature: 121°C and 134°CŸ Quantity: 1

MicropipettesŸ Volume: 2-20μl, 20-200μl,100-1000μl, Ÿ Autoclavable at 121°CŸ Quantity: 2-4 sets (2 sets spare for autoclaving)8 Channel Pipette and Single Channel PipetteŸ Single Channel Volume: 2-20μl Ÿ 8 Channel Volume: 5-50μlŸ Quantity: 1-2 sets (1 set spare for autoclaving)

Advanced Pipette Filler E-PF-PŸ Volume: 0.1-100mlŸ Quantity: 1Repeatable PipetteE-RP-AŸ Volume: 1-5000μlŸ Quantity: 1

Quantitative Real-Time PCR E-QPCR4-CIŸ Capacity: - 96 x 0.2ml PCR Tubes - 12 x 0.2ml PCR 8 Strips - 96 PCR Well PlateŸ Wavelength Range: 455-650nm/510-715nm excluding Cy3 Ÿ Fully Computer Integrated Operation (Computer Excluded)

Designed in accordance with the 2019-nCoV Nucleic Acid Test SOP released by Peking Union Medical College Hospital ( ).https://www.pumch.cn/en.html

Disclaimer: Quantities may vary depending on the COVID-19 test kit used, number of laboratories (2-3) in sequence and the procedure adopted.

Equipment Required Product Code Qty

Advanced pipette filler E-PF-P 1

Micropipettes

E-MPA-AV20 6

E-MPA-AV200 4

E-MPA-AV1000 4

8 Channel pipette E-MP8A-AV50 2

Pipette stand E-MP-LS6 3

Repeatable pipette E-RP-A 1

Autoclave E-SA-300VF 1

Bio-safety cabinet level 2 0.9/1.2/1.5m 3

Computer Windows 10, core i5 2.5GHz,SSD 1

High speed micro centrifuge E-C15-12.2 1

Medical refrigerator 2 to 8°C Meiling YC-315L 1

Medical refrigerator 2 to 8°C & freezer -10 to -26°C Meiling YCD-EL289 1

Nano centrifuge E-C7-Nano 2

Real time PCR E-QPCR4-CI 1

Shaking dry bath E-DB100S-P 1

Vortex mixer E-VM-A 3