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L-GLUTATHIONE (For Health Food)
Copyright 2012 The KOHJIN Co., Ltd. All rights reserved. No reproduction or republication without written permission.
Manufactured by ;
Phone : +81-3-3242-3021 FAX +:81-3-3242-3087
1-21, Nihombashi Muromachi 4-Chome, Chuo-Ku
Tokyo, 103-0022, Japan
URL : http://www.kohjin.co.jp/english
Glutathione, Resistance for Digestion
� Resistance Study with Artificial Digestive Juices
We checked the resistance of Glutathine (GSH) against
digestive juices with artificial digestive juices.
We used�
Pepsin(1:50) for 1hr
SIF(1:250) for 2hr
Amino peptidase(200mU/mg) for 2hr
Then we measured GSH by HPLC (Data not shown for confidential).
→We expected that the Glycine of GSH would be digested,
but unexpectedly, no Glycine had digested.
→For oral intake, intact GSH will reach to small intestine.
2
Absorption Study (Oral Intake)
� Experimental Design
� Intact Glutathione pass through the Caco-2 monolayer.
3
Caco-2
monolayer
1mM GSH
Apical
chamber
Basolatera
l chamber
Analyze by GSH assay
and LC/MS
Trans well: Using Chamber:
(Data not shown for confidential)
4
Skin Health & Glutathione
� Function of Glutathione & Benefits�
Melanin
Inhibition
Antioxidant
� Whitens the skin
� Dark spot remover
� Prevent and/or remove pimples
and pimple marks
� Anti-aging and anti-wrinkles
� Makes skin smooth, fresh and
radiant
� Enhance healing of wounds
Mechanism of Skin Beauty
� Glutathione inhibit the Tyrosinase activity and Eumelanin synthesis and increase the ratio of Pheomelanin
L-Tyrosine
L-Dopaquinone
Eumelanin
Tyrosinase
Dark Complexion
Pheomelanin
Skin Brightness
Tyrosinase
Glutathione inhibits
Tyrosinase activity
5
Glutathione
promotes
Pheomelanin
synthesis
Clinical Study on Skin Beauty
� Terms of the study: May to July, 2010
� Subjects of the study: Thirty (30) women, age 20s to 40s
� Study groups: Placebo group & Glutathione group
� Sample Glutathione: 50mg, once a day, before sleep
� Study protocol: DBT / Parallel Group Comparison
Apply UVs to skin on 4th week, and check following items;
Moisturizing, Erythema, L-value(brightness), Questionnaires
6
*Intake sample
Apply UVs to skin
Start 4th week 8th week
Moisturizing & Erysma Recovery
� 4 weeks later after apply UVs (8th week), we checked
the moisturizing and recovery rate of erythema (reddish)
increases.
7
0
10
20
30
40
50
60
70
start 4 weeks
Recovery from erythema formation
from UV radiation (Elbow)
YH-15 Placebo
*YH-15 = KOHJIN Glutathione
0
2
4
6
8
10
12
14
16
18
0 time 4 weeks 8 weeks
Moisturizing effect (Left cheek)
YH-15 Placebo
(Points) (%)
L-Value Changes with Time
� 4 weeks later after apply Uvs (8th week), we checked
the L-Values as brightness.
8
-0.5
0
0.5
1
1.5
2
UV radiation 4 weeks
Brightness(Shoulder)
YH-15 Placebo
*YH-15 = KOHJIN Glutathione
(Points)
Questionnaires on Skin Beauty
� Questionnaire survey after 4 weeks and 8 weeks of
the test period.
9
-0.5
0.5
1.5
2.5dullness
spots
texture
clearness
moisture
dryness
dryness (lips)
ease of makeup
Survey after 4weeks of test period
YH-15 Placebo
-0.5
0.5
1.5
2.5dullness
spots
texture
clearness
moisture
dryness
dryness (lips)
ease of makeup
Survey after 8 weeks of test period
YH-15 Placebo
*YH-15 = KOHJIN Glutathione
10
Detoxifier Effect
� Detoxification mechanism of Glutathione.
Toxin Excretion
Normal
Liver
Activated Liver
GSH：L-Glutathione Low Glutathione Level Liver�
Toxins
(Heavy Metal)
(Alcohol)
ToxinsGSH GSH
GSH
ToxinsGSH GSH
GSH
GSH
GSH
GSH
GSH
GSH
GSH
GSH
GSH
GSH
GSHGSH
GSH
GSH
GSH
GSH
Toxins
GSH
GSH
GSH
Toxins
GSH
GSH
GSH
Glutathione Supplementation!!
ToxinsGSH GSH
GSH
In-House Human Study (Alcohol)
� Sample;
Placebo(Dextrin), Curucmin (30mg) and L-Glutathione (100mg)
� Subjects; N=20 (Men and Women)
� Alcohol intake; 1.25mL X Body Weight (kg)
� Measure the alcohol concentration in breath on 20min,
60min 120min and 180min and Questionnaires.
11
↓Intake alcohol
0 20 60 120 180 (min)
Measure Alc. ○○○○ ○○○○ ○○○○ ○○○○
Questionnaire ○○○○ ○○○○ ○○○○
↓Intake samples
-60
12
In-House Human Study (Alcohol)
� Glutathione reduced the alcohol concentration in
breath immediately.
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
20min 60min 120min 180min
Alc
ohol concentr
ation in b
reath
(m
g/L
)Control
Curucmin (30mg)
Glutathione (100mg)
a
b
a,b
a
c
a,c d
dd
d
1.0p<0.01 (a-a, b-b), p<0.05 (c-c, d-d)
after drinking (1.25mL alcohol × kg BW).
13
In-House Human Study (Alcohol)
� Glutathione improved the Physical Feeling of
Alcoholic Sobered Up.
� Alcoholic Sobered Score; Improvement score of
sobering up, sleepiness, headache, heavy stomach,
swelling, glow and dizzy (Average).
Control
Curucmin (30mg)
Glutathione (100mg)
60 120 180 (min)
Alc
oholic
Sobere
d U
p S
core
In-House Human Study (Liver Health)
� Sample;
Placebo(Dextrin) and L-Glutathione (50mg/day)
� Subjects; Total N=26 (Men and Women)
� Term; 8 weeks
� Collect the blood and measure the AST, ALT and γ-GTP on
0 week and 8th week.
14
*Intake sample (50mg/day)
Start 4th week 8th week
↑Collect blood ↑Collect blood ↑Collect blood
In-House Human Study (Liver Health)
� Glutathione reduced AST level significantly.
� Glutathione also reduced ALT level significantly.
15
■■■■Placebo ■■■■Glutathione
Change rate of ALT
a
a, b
b
Ch
an
ge
ra
te (
%)
Change rate of AST
■■■■Placebo ■■■■Glutathione
a
a, b
b
In-House Human Study (Liver Health)
� Glutathione has a tendency to improve γ-GTP level.
� Glutathione improve liver function significantly!!
16
■■■■Placebo ■■■■Glutathione
Change rate of γ-GTP
Ch
an
ge
ra
te (
%)
17
is world No.1 share
Kohjin’s L-Glutathione is�
� US FDA GRAS Notice (#GRN 000293)
� KOSHER Certified by “OK”
� HALAL Certified by “MUI”
(for Muslims such as Indonesia, Malaysia, Middle Easts�)
� Non-GMO Product
� Fermented from FDA Approved Yeast (Torula Yeast)
� No Adverse Report on Drug Compatibility
Kohjin is�
� Leading Manufacture of Glutathione since 1968
� ISO Certified / GMP approved
(Product Example)
The consumer awareness of Glutathione is very high in
South East Asia as a whitening ingredient, and also in Japan,
Korea, China as a Detoxifier/Anti Hung-over/Liver Health.
