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KVRI KR Burbot Subcommittee Meeting October 7, 2008
Nathan R. JensenUniversity of Idaho\Fish and Wildlife [email protected]
UI Burbot Aquaculture Progress 2008
KVRI 2000
Introduction• Where we left off 2007 • 2008 Goal and
Objectives
2008 Summaries: • Observational studies• Production
Presentation outline
1 mm
Where we left off 2007
Spawning:• Expect volitional spawning to occur w/wo hormone.
Incubation:• Increase number of Imhoff cones.• Treat eggs with Iodine during water hardening.
Larval feeding:• Expand live feed production.• Feed live prey >50 days before transition.• Hand feed commercial diets and explore other diets.
Artificial pond culture:• Graduate student (MS) project.
Cryopreservation:• Continue establishing germ plasm repositories at UI.
Goal:• Produce 5,000 commercial diet
transitioned burbot.
Observations:• Observe Ovaplant affect on
spawning behavior.
• Observe sensitivity of water hardening eggs to Ovadine.
• Compare Otohime and INVE larval weaning diets.
2008 Goal, Observational studies
1. Further observe Ovaplant affect on spawning behavior
• 20 females observed:
a. 10 females given Ovaplant injections.
b. 10 females not Injected.
Objective 1 – Spawning observation
2. Observe water hardening egg sensitivity to Ovadine
• 11 spawns included in study
a. Treated eggs with 0, 25 or 50ppm Povidone Iodine
b. Determined percent live eggs at 48 hours; 3 samples per incubator
Objective 2 - Ovadine observation
3. Compare Otohime and INVE larval diets
• 4600 larvae stocked into each of four tanks after 10 weeks of live diet feeding
a. Larval weaning diets fed six weeks
b. Survival, growth, length, cannibalism compared
Objective 3 - Larval feed trial
Spawning behavior summary :
Ovaplant injected:• 100 % spawned• 0 % rest year
No Oviplant:• 80 % spawned• 20 % rest year
Spawning observation results
Spawning behavior summary:
Ovaplant injected:• 70 % volitionally spawned• 30 % spawned manually
No Oviplant:• 30 % volitionally spawned• 50 % spawned manually
Spawning observation results
Ovadine observation results
Note: No statistical analysis because not all treatments applied to all individual spawns.
Ovadine Treatment-Moyieeggs-all 11 spawns
0 ppm
25 ppm
50 ppm0
25
50
75
100
Ovidine concentration
Perc
en
t fe
rtil
izati
on
Larval feed trial results - survival
NOTE: No significant difference 31 d or 46 d post dry diet introduction; p = 0.0634 and 0.0657 respectively (n=2).
Larval feed trial results – growth
Relative growth rates:
Per day: • INVE = 0.15 mm. • Otohime = 0.13 mm.
Per month: • INVE = 4.6 mm. • Otohime = 4.0 mm.
Larval feed trial results - length
Mean (n=20) TL of larvae at theend of feed trial
INVE Otohime0
10
20
30
A
B
Diet
mm
NOTE: Larval lengths were found significantly different; p = 0.0051.
Larval feed trial - Cannibalism
Cannibals per tank:
• INVE = 23 and 24.• Otohime = 10 and 14.
Relative percent at end of trial in each tank:
• INVE = 2% and 7%.• Otohime = 1% and 2%. Cannibals were removed when observed.
Production Overview 2008
Outline:
1. Spawning
2. Incubation
3. Live feeds
4. Larval / Juvenile survival
5. Cryopreservation
Spawning 2008
Spawning results:
• 16 spawning events occurred.
• 12 events were volitional.
• 5 females had rest year.
• 21 of 25 males produced milt.
Spawning 2008
Egg collection results:
• 7.6 M eggs were collected.
• Fertilization averaged 84%;o range 38 - 99%.
• 6.7 M eggs became fertilized.
Incubation 2008
Incubation results:
• Water temperature 3-5˚ C.
• Hatches began after ~34 d;o range 28 - 41 d.
• Hatching lasted ~13 d;o range 4 - 21 d.
• 55,000 cripples were culled.
Cryopreservation 2008
• Milt samples were cryopreserved from all 2007 captures.o Four sets of samples were cryopreserved.
NOTE: All Moyie males captured to date are represented in cryo-storage (c/oSteve Patton; UI Biological Science Department .
NOTE: For current inventory contact UI BioSci Department .
2008 burbot semen sampling:
Research support overview 2008
UI Research: • 109,000 eggs used for egg fungus control experiment. • 30,000 feeding larvae to extensive rearing experiment. • 600 feed trained fingerlings to disease susceptibility experiments.
IDFG Research:• 56,000 feeding larvae used for extensive rearing observations.
Future research:• Submitted 3 early life developmental sets to CSU Larval fish laboratory. c/o Darrel Snyder, curator.
Implications and plans for 2009
Spawning and gamete production:1. Collect gametes from wild rather than new adults.
2. Continue use of hormone implants to promote repeat spawning.
Live feeds production:1. Rotifers: target production = 100 M per day.
2. Artemia: target production = 50 M per day.
3. Incorporate automated live feeds injection systems.
Implications and plans for 2009
Experiment with larval diets:1. INVE (Lansy / EPAC diets)2. Otohime (β and C diets)3. Skretting (Gemma micro diets)
Continue supporting UI graduate and IDFG research:1. Extensive rearing experimentation.2. Temperature related growth, survival and condition
experimentation.
There are no plans to cryopreserve milt in 2009.
Funding and Support
Support:Kootenai Tribal Fish Hatchery
BC Ministry of Environment
Idaho Department of Fish and Game
University of Idaho
KVRI
Funding :Kootenai Tribe of Idaho and The Bonneville Power Administration Project:198806400; Contracts:20490, 25349
Contact Information:Nathan R. [email protected]