oleh:Elina Lestariyanti S. Pd

PLANT TISSUE CULTURE

JOURNAL

REFERENCES

Plant Tissue Culture in Biotechnology:Recent advances in Transformation Through Somatic Embryogenesis

Agrobacterium -Mediated Transformation

Particle Bombardment

Somatic Embryogenesis

PARTICLE BOMBARDMENT

Methods for DNA delivery into plant cell, were used for specific transformation purposes and different types of cell and genotypes. (1)

- Electroporation- PEG (Polyethylen

Glycol)treatment - Microinjection - Sonication- Silicon carbide

treatment

Helios Gen Gun dan alat Gen Gun Standar

Agrobacterium -Mediated Transformation

Method for transferring genes into plants through the inoculation of leaf discs with Agrobacterium tumeficiens followed by in vitro culture and regeneration of whole plants. (1)

WHY Agrobacterium

?crown gall tumor

Agrobacterium tumefaciens is a natural vector system for transgenes delivery into a wide range of plants species. (1)

SOMATIC EMBRYOGENESIS

Somatic embryogenesis is a process whereby somatic cells differentiate into somatic embryos. (2)

In somatic embryogenesis the embryos regenerate from somatic cells, tissue or organs.

Soybean Transformation

Fig. 1. Scheme for isolation of immature cotyledons from a soybean pod (A), induction of somatic embryos on MS40 medium (B) and embryogenic callus on MS20 and FN Lite media (C, D). Bar: 1 mm

Fig. 2. A — Transient GFP expression in soybean embryogenic callus (1 day after bombardment with NPTII/GFP construct). B, C, D — GFP expression in single somatic embryo, callus and mature somatic embryos obtained from embryogenic callus after 9 days of culture on SHAM medium. E, F Maturation of somatic embryos in suspension culture and their germination.

Cotton Transformation

Fig. 3. Different stagesof cotton hypocotyls transformation

Fig. 4. Agrobacterium-mediated transformation

of embryogenic callus:

(1) Sidorov, V. A. 2013. Plant Tissue Culture in Biotechnology: Recent Advances In Transformation Through Somatic Embryogenesis. Biotechnologia Acta. 6(3).

(2) Arnold, S.V. 2008. Somatic Embryogenesis. In Plant Propagation by Tissue Culture 3rd Edition. E. F. George, et al. (eds). Netherland: Springer. pp 335.

(3) Purnamaningsih, R. 2002. Regenerasi Tanaman melalui Embriogenesis Somatik dan Beberapa Gen yang Mengendalikannya. Buletin Agrobio. 5(2): 55.