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Key cultivation techniques for hemp Stefano AMADUCCI Institute of Agronomy, Genetics and Field crops [email protected] Summer School Lignocellulosic Crops as Feedstock for Future BiorefineriesLisbon, 26-31 July 2014

Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

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Page 1: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Key cultivation techniques for hemp

Stefano AMADUCCI

Institute of Agronomy, Genetics and Field crops

[email protected]

Summer School

“Lignocellulosic Crops as Feedstock for Future Biorefineries” Lisbon, 26-31 July 2014

Page 2: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Study material:

Key cultivation techniques for hemp

Page 3: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Layout of the lecture:

• Introduction;

• Hemp botany, biology and resource use efficiency;

• Influence of agronomic factors on industrial hemp;

• Conclusion.

Key cultivation techniques for hemp

Page 4: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

C. erratica Anders

C. indica Lamark

C. macrosperma Stokes

C. chinensis & C. gigantea Delile

C. gigantea Crevost

C. erratica Siev.

C. foetens Gilib

C. lupulus Scop.

C. americana Pharm

C. generalis E.H.L. Krause

C. ruderalis Janischeskii

Fam. Cannabaceae

Cannabis sativa L.

(Anderson, 1980)

C. sativa

C. indica

C. ruderalis

(Small, 1974)

C. sativa subsp. indica

subsp. sativa

Page 5: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Chemotype I: (drug) THC > 0.3 % D.W.

CBD < 0.5 % D.W.

Chemotype II: (intermediate) THC 0.3 % D.W.

CBD > 0.5% D.W.

Chemotype III: (fibre) THC < 0.3% D.W.

CBD > 0.5% D.W.

Definizione dei chemotipi

SMALL & BECKSTEAD, 1973

Page 6: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Introduction

Origin and diffusion

1545

4-8000 BC

1606

1755 Centre

of origin

Page 7: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Introduction

Origin and diffusion

Page 8: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Introduction

Origin and diffusion

Page 9: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Introduction

Potential uses of hemp

Page 10: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Scheme of the

MULTIHEMP

Biorefinery

concept

www.multihemp.eu

Page 11: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Introduction

Actual European uses of hemp fibre

Page 12: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Introduction

Actual European uses of hemp shives

Page 13: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Introduction

Actual European uses of hemp seeds

Page 14: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Page 15: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Page 16: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Young stem/internode Old stem/internode

Hemp botany, biology and resource use

efficiency

Page 17: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Page 18: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Fibre cell maturity

Hemp botany, biology and resource use

efficiency

Middle lamella Primary cell wall

Layers of secondary cell wall

Page 19: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

15-50 µm

20-80 mm

Page 20: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Dioecious

Page 21: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Monoecious

Page 22: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Hemp phenology

Amaducci, S. et al, 2008. Eur. J. Agron., 28, 90-102.

BVP PIP FDP

*

Page 23: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Hemp phenology

Amaducci, S. et al, 2008. Eur. J. Agron., 28, 90-102.

BVP FDP

Page 24: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Hemp phenology

Amaducci, S. et al, 2008. Eur. J. Agron., 28, 90-102.

PIP

Page 25: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Page 26: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Page 27: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Struik, et al., 2000. Industrial crops and products, Vol. 11/2-3, 107-118

50 100 200

RUE 1-9

RUE 1-7

RUE 7-9

K

1.92

2.30

0.86

1.02

1.83

2.26

0.95

0.93

1.72

2.12

0.44

0.86

Plant density (n m-2)

Radiation Use Efficiency

Page 28: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Cosentino et al., 2013. Ind. Crops Prod. 50, 312–324.

Water Use Efficiency

1.91 - 2.48 g biomass L−1 beginning of flowering

2.73 - 3.45 g biomass L−1 beginning of flowering

Page 29: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Hemp botany, biology and resource use

efficiency

Nitrogen Use Efficiency

Page 30: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Agronomic factors

• Soil preparation

• Variety choice

• Sowing time

• Plant density

• Plant nutrition

• Crop rotation and weed management

• Irrigation

• Harvesting time

Page 31: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Agronomic factors

• Soil preparation

• Variety choice

• Sowing time

• Plant density

• Plant nutrition

• Crop rotation and weed management

• Irrigation

• Harvesting time

Crop parameter

Biomass yield

Page 32: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Agronomic factors

• Soil preparation

• Variety choice

• Sowing time

• Plant density

• Plant nutrition

• Crop rotation and weed management

• Irrigation

• Harvesting time

Crop parameter

Fibre yield

Page 33: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Agronomic factors

• Soil preparation

• Variety choice

• Sowing time

• Plant density

• Plant nutrition

• Crop rotation and weed m.

• Irrigation

• Harvesting time

Crop parameter

Fibre quality:

• Primary/secondary fibre

• Degree of maturity

• Fibre diameter

• Fibre lenght

• Lignification

Page 34: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Agronomic factors

• Soil preparation

• Variety choice

• Sowing time

• Plant density

• Plant nutrition

• Crop rotation and weed management

• Irrigation

• Harvesting time

Crop parameter

Fibre quality

Page 35: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Variety choice: Effect on flowering time

Page 36: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Variety choice: …and on stem yield

Page 37: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Cultivars bred in northern environments have lower

biomass yield when grown in the South, mainly due to

shortened growth duration and early-flowering

Finola in Latvia Finola in Italy

Variety choice – Effect on flowering time

Page 38: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Variety choice – Effect on flowering time

Amaducci, et al., 2012. Industrial Crops and Products, 37, 100-112

Page 39: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Amaducci, et al., 2005. Journal of Industrial Hemp, Vol. 10 (1), 31-48.

Effect of variety and sowing time on flowering time

Page 40: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Plant density – Effect on stem yield

Amaducci, et al., 2002. Journal of industrial hemp, Vol. 7 (2), 33-60

Page 41: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Actual hemp cultivation and future

perspectives

Plant density – Effect on yield

Amaducci, et al., 2002. Journal of industrial hemp, Vol. 7 (2), 33-60

Page 42: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Plant density – Effect on stem yield

Amaducci, et al., 2002. Journal of industrial hemp, Vol. 7 (2), 33-60

Page 43: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Amaducci, et al., 2002. Journal of industrial hemp, Vol. 7 (2), 33-60

Page 44: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Amaducci, et al., 2002. Journal of industrial hemp, Vol. 7 (2), 33-60

Page 45: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Plant density – Effect on stem yield

Extinction of incident PAR (%)

0

20

40

60

80

100

6-Apr 16-Apr 26-Apr 6-May 16-May 26-May 5-JunDate

PA

R E

xti

nc

tio

n

D30

D45

D90

D180

D270

Amaducci, et al., 2002. Journal of industrial hemp, Vol. 7 (2), 33-60

Page 46: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Amaducci, et al., 2005. Journal of Industrial Hemp, Vol. 10 (1), 31-48.

Plant density – Effect on fibre lenght?

Page 47: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Amaducci, et al., 2005. Journal of Industrial Hemp, Vol. 10 (1), 31-48.

Plant density – Effect on fibre yield

Page 48: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Amaducci, et al., 2005. Journal of Industrial Hemp, Vol. 10 (1), 31-48.

Plant density –

Effect on fibre yield

and quality

Page 49: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Amaducci, et al.., 2008. Field crops Research, 107,161-169.

Page 50: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

Influence of agronomic factors on

industrial hemp

Amaducci, et al., 2005. Journal of Industrial Hemp, Vol. 10 (1), 31-48.

Plant density x Harvesting time – Effect on fibre yield

Page 51: Key cultivation techniques for hemp - fibrafp7.net · Key cultivation techniques for hemp ... Fam. Cannabaceae Cannabis sativa L. (Anderson, ... effect of environment and cultivation

A relatively large scientific literature dealing with the

effect of environment and cultivation practices on

hemp production is available, while limited

information is available on the effect of environment

and management on plant quality characteristics (of

fibre, seed and secondary metabolites).

Conclusion

Conclusion