Kefir administration reduced Kefir administration reduced progression of renal injury in STZprogression of renal injury in STZ--

diabetic ratsdiabetic rats

Cristina Stewart Bogsan

Pharmaceutical-Biochemical Technology Department

OutlineOutline

• Diabetic Mellitus;

• Kefir;

• Oxidative stress;

• Aim;• Aim;

• Protocol;

• Results;

• Conclusion.

Diabetes MellitusDiabetes MellitusDiabetes Mellitus (DM) is a

group of metabolic disorders thathave in common the hyperglycemia.

DM has become a seriouspublic health problem that affectspublic health problem that affectsmillions of individuals worldwide(Shaw, 2010).

30% of patients developnephropathy, the main cause ofmorbidity and mortality in diabeticpatients (Molitch et al., 2004).

Source: Shaw, 2010

HYPERGLYCEMIA

Increased ROS production

Impaired NO bioavailability

NF-kB Activation

Oxidative Stress in DMOxidative Stress in DM

bioavailability

Lipid peroxidation (LPO)

Endothelial dysfunction

Malandialdehyde (MPA)

Thiobarbituric acid substances (TBARS)

NF-kB Activation

Pro-inflamatorybiomarkers

Increase C-reactive protein (CRP)

KefirKefir• Kefir is a fermented milk that contains a complex

symbiotic mixture of Lactic Acid Bacteria (LAB) and

Molds.

• The main microorganisms are:• The main microorganisms are:o Lactobacillus,

o Lactococcus,

o Leuconostoc,

o Streptococcus,

o Kluyveromyces,

o Saccharomyces,

o Torula.

Kefir propertiesKefir properties

Kefir is known for providing benefits to human

health through its anti-inflammatory, immune-

stimulatory and antioxidant properties.

AIMAIM

This study aimed at assessing the effects of Kefir on

oxidative stress in diabetic animals.

STZ - diabetic induction45 mg/kg iv

Skimmed Milk

Fermentation until pH 4.6

20mg/ 100mL Kefir DA Inoculation

Kefir preparation

Stiring(1min)

Distribution in 50 mL cups

Cooling in ice bath

Storing (4 °C)

Kefir fermented milk

ProtocolProtocolSTZ Kefir

5 days 24 hs

Sacrificed

24 hs 8 weeks

Kidney histology

CTLCTL CTLKCTLK DMDM DMKDMK

Kefir groups intake 1.8 mL/day by gavageWater to CTL and DM groups

Water and Chow intakeDiuresis, WeightFasting Blood toleranceCreatinine,Proteinuria, Tbars, NO

CTLCTL CTLKCTLK DMDM DMKDMK

Water and Chow intakeDiuresis, WeightFasting Blood toleranceCreatinine,Proteinuria, Tbars, NO

Metabolic profile, renal function, and Metabolic profile, renal function, and oxidative stressoxidative stress

Metabolic profile, renal function, and Metabolic profile, renal function, and oxidative stressoxidative stress

GlycemiaGlycemia levelslevels

Oral Glucose Tolerance TestOral Glucose Tolerance Test

Renal functionRenal function

Renal functionRenal function

Oxidative StressOxidative Stress

Oxidative StressOxidative Stress

Inflammatory biomarkerInflammatory biomarker

Kidney histologyKidney histology

ConclusionConclusion

The results obtained in this study show that Kefir

treatment significantly reduced the progression of STZ-

induced hyperglycemia and oxidative stress in rats.induced hyperglycemia and oxidative stress in rats.

Kefir may play a role in slowing the metabolicchanges that contribute to DM as a non-pharmacological adjuvant .

AcknowledgmentsAcknowledgments

UnifespElisa M.S. Higaa,

Giovana R. Punaroa,

Fabiane R. Maciela,

Adelson M. Rodriguesa,

Silvia S.M. Iharab,

This work was supported by:

Coordenação de

Aperfeicoamento de Pessoal

de Nivel Superior (CAPES),

Silvia S.M. Iharab,

Sergio R.R. Araujob

USPMarcelo M. Rogeroc,

Marice N. Oliveirad,

Talita R.C. Sanchese,

Lucia C. Andradee,,

Fundação de Apoio a

Universidade Federal de São

Paulo (FAP-Unifesp),

Fundação de Amparo a

Pesquisa do Estado de São

Paulo (FAPESP).

Thank youThank youcris.bogsan@usp.br