Juan-Rodriguez-SCIA-AutismOne-2013 (1)

2013

Microglial Activation in Autism

Closer Than Ever To Finding A Cure

Microglia – The brain's immune system

cell behind autism.

www.stopcallingitautism.org www.stopcallingitautism.org\espanol 1

https://www.google.com/url?sa=i&rct=j&q=&esrc=s&frm=1&source=images&cd=&cad=rja&docid=zlWURhsJ7bOtqM&tbnid=-_b8B9I7m_pPMM:&ved=0CAUQjRw&url=https%3A%2F%2Ftwitter.com%2FAutismOne&ei=17KiUY8pjvzxBICbgZAK&bvm=bv.47008514,d.dmQ&psig=AFQjCNFUHYVHbf59x3MbgKZDnlU9OxsbEQ&ust=1369703502034504

Agenda

• Video: Daniel’s journey through autism

• What is autism

• The role of microglial activation in autism

• How to block microglial activation In autism

• Making treatments that block microglial activation available at your local pharmacy

• Tips To Prevent Autism

2 www.stopcallingitautism.org www.stopcallingitautism.org\espanol

Who is Juan Rodriguez?

• Born in Mayaguez, Puerto Rico

• Happily married with Sandra Rodriguez

• Three sons, Daniel (8yrs), Adrian (7yrs) and Lucas (20mos)

• Became a medical researcher while seeking help for his son Daniel

• Founder and President of Stop Calling it Autism!

• Founder of Glial Technologies LLC www.glialtechnologies.com

• Author of a medical article that provides an end to end explanation of the autism disease process

• Discovered a medical treatment that blocks microglial activation in autism


http://www.glialtechnologies.com/

Daniel’s Story

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Daniel before autism Daniel with autism Daniel – cured from autism

www.stopcallingitautism.org www.stopcallingitautism.org\espanol


Video of Daniel’s journey through autism

Link to Video

http://www.youtube.com/watch?v=yN_O7OI1lb0

Autism According to the CDC

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The Center for Disease Control and Prevention (CDC) provides the following facts in regards to autism Definition • Autism is developmental disorder that can cause significant social,

communication and behavioral challenges.

Diagnosis • Autism can be very difficult to diagnose since there is no medical test,

like a blood test, to diagnose the disorders. Doctors look at the child’s behavior and development to make a diagnosis.

Causes • We do not know all of the causes of autism.

Treatment • There is currently no cure for autism.

CDC – “There is currently no cure for ASDs and we do not know all the causes of ASDs.”

Logo courtesy of the Center for Disease Control and Prevention (CDC)


Autism Statistics in the United States

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The incidence of autism continues to grow at a very alarming rate.

Graph courtesy of Autism Speaks

Based on Data from February 2007 • Autism affects 1 in 150 children

(1 in 110 in 2009, 1 in 88 in 2011 and now 1 in 50 children are now affected in 2013!

• Annual cost in the United States is 90 billion dollars (90% of the cost for adult care and services)

• It is the fastest growing developmental disorder in the USA

• In 10 years (2017), the annual cost of autism will be $200-400 billion


http://www.autismspeaks.org/docs/Prevalence_Graph_12_18_2009.pdf

Autism According to SCIA

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Stop Calling It Autism! (SCIA) provides the following facts in regards to autism

Definition • A medical illness in which the brain’s immune system (microglia) attack the

connections in the brain. This leads to developmental delays that can cause significant social, communication and behavioral problems

• Children with autism often suffer from many other medical illnesses that are associated with microglial activation.

Diagnosis • Autism can be diagnosed with blood tests that are commonly used by doctors to

diagnose infections, inflammation and immune system dysfunction • Brain SPECT scans can also be used to determine the areas of the brain affected

by microglial activation

Cause • Autism is caused by microglial activation.

Treatment • Medications that can block microglial activation and keep infections under

control.

Autism can be diagnosed with blood tests and brain SPECT scans.


http://www.stopcallingitautism.net/brainspectscans.html

The New Frontier in Brain Science

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• Everything that is known and said about autism and other mental illnesses is rapidly changing as we speak

• Through the understanding of the role of the microglia in health and disease, many mental conditions including autism can be cured

• Many world renown scientists are now beginning to

understand how important this new science is

• Daniel was the first child to be completely cured from autism by following the science that will be explained in this presentation.

• Many children are now following Daniel’s footsteps.


cell behind autism.


Glial Research Is Growing Very Quickly


DOAJ – Directory of Open Access Journals

http://www.doaj.org/doaj?func=search&template=&uiLanguage=en&query=microglia

The Function of the Glial Cells

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What are glial cells? • Most common cells in the central nervous

system (CNS) • They provide support and protection for the

neurons in the brain • Control communication between neurons • Control blood flow in the brain • There are three types of glial cells: astrocytes,

oligodendrocytes and the microglia

Types and functions of the glial cells • Microglia – brain’s immune system cells, they

are in charge of neurotransmission • Astrocytes – in charge of neurotransmission

(similar to the microglia) and they also provide metabolic support to the neurons

• Oligodendrocytes – produce myelin which acts like an electrical wire insulation in the neurons

Image Courtesy of “N. Allen and B. Barres, 2009”

The microglia and the other glial cells play a central role in brain function


The role of microglial cells in normal brain development

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Microglial Cells are essential in brain development • Microglial are not only are our brain’s

immune system cells • They influence synaptic development

and connectivity. • They regulate brain development by

microglia do the trimming of the extra synapses

Image Courtesy of “Kettenmann and Kirchhoff, 2013”

Microglial cells are not only our brain’s immune system cells, but also are essential in the normal and developing brain. They influence synaptic development and connectivity.


The figure above shows the dynamic interaction of microglial processes with the tripartite synapse(A) Microglial processes (red) dynamically contact the cellular compartments of the tripartite synapse: pre- and postsynaptic neuronal terminals (in brown) as well as the enwrapping perisynaptic astroglial process (in blue).(B) The electron micrograph (EM) specifically shows a microglial process (m) contacting both the pre- and postsynaptic compartment.

Microglial Activation Leads to Brain Dysfunction In Autism

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Microglial cells when activated wrap too many brain connections and destroy them.

When the microglial cells become chronically activated they destroy too many brain connections. In autism this leads to brain underconnectivity and dysfunction.


The figure above shows the synaptic pruning by microglial processes. (A) The stability and maintenance of presynaptic terminals and postsynaptic spines is determined by microglia in a three-step process called synaptic pruning composed of contact, engulfment, and phagocytosis of presynaptic terminals. (B) The electron microphotograph shows ultrastructural interactions between microglia (red) and synapses (brown) in the mouse visual cortex.

Image Courtesy of “Kettenmann and Kirchhoff, 2013”

Microglial Activation In Autism

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Microglial Activation is at the center stage of all common neurological and medical problems in autism. From speech and behavioral disorders to ezcema, food allergies, GI issues, frequent infections, seizure activity, sensorial problems to eating disorders.


Microglia – The brain's immune system cell behind autism.

Role of glial cells in the gut issues in autism

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Many individuals with autism often suffer from GI issues including food allergies, constipation, diarrhea, intestinal bowel disease (IBD), irritable bowel syndrome (IBS), etc… Often referred to as the “second brain”, the enteric nervous system (ENS) controls the function of the digestive tract. The enteric nervous system can operate independently from the brain Similar to the neurons found in the brain, the neurons in the enteric nervous system, are surrounded by glial cells. Glial cells in the central nervous system (CNS) can communicate with the glial cells in the enteric nervous system (ENS)

The enteric nervous system is composed of both neurons and glia. Recent evidence indicates that enteric glia–which vastly outnumber enteric neurons–are actively involved in the control of gastrointestinal functions

The role of enteric glia in gut inflammation

Glial cells in the gut


http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8487428

http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2982.2005.00687.x/pdf

Role of glial cells in the gut issues in autism

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The glial cells in the digestive system are known as enteric glial cells (EGCs) EGCs can be both protective and destructive in the gut EGCs have an important role in maintaining the integrity of the mucosal barrier of the gut EGCs are involved in basic gut functions, such as the regulation of peristalsis, blood flow and the modulation of the immune and inflammatory processes In autism, glial cells in both the brain and in the digestive system don’t work correctly causing the neuron underconnectivity that leads to the neurological and GI symptoms

Peristalsis

Enteric Glial Cells

The glial cells in the digestive systems are known as enteric glial cells


Peristalsis are a series of muscle contractions that occur in your digestive tract

Are children with autism different?

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Children with autism suffer from different neurological and medical problems, but the root cause is the same… Microglial Activation

• Seizures - Microglial activation exacerbates seizure activity • Motor Skills Disorders - is a result of weak or disorganized

connections in the brain. • Mitochondrial Disorder – Elevated levels of nitric oxide (NO)

inhibits mitochondrial respiration of surrounding neurons • Frequent Viral, Fungal, Parasitic and Bacterial Infections –

Elevated levels of NO may reduce and impair natural killer (NK) cell function

• Eating Disorders - Alteration of the NO plays a role in eating disorders

• Asthma and Allergies – Elevated levels of NO are found in patients suffering from Asthma and Allergies

• Eczema – NO is an important player in eczema • Reduced Cerebral Blood Flow – caused by excessive contraction

of brain blood vessels due to astroctytic activation

Natural Killer Cells

Natural killer cells (NK cells) are a part of the immune system that plays an important role in eliminating cancer cells, viruses, bacteria, fungus and parasites from the body.

Microglial cells control the communication between neurons

Microglia

Nitric Oxide

Nitric Oxide is a gas naturally found in the body. NO is an important mediator and regulator of many processes in the nervous, immune and cardiovascular systems.


Synapses Destruction Caused by Microglial Activation

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Microglial Activation also occurs in Alzheimers patients - link to video

Video courtesy of TheHealthScience's Channel By Dr. Joseph Rogers – Banner Sun Health Research Institute

Video showing how during chronic microglial activation too many brain connections (synapses) are destroyed leading to brain underconnectivity and reduced brain function

The immune cells known as microglia, which were thought to be active in the brain only in the presence of diseases or infections are constantly active, they probably play a central role in one of the most basic functions in the brain – the creation and destruction of the synapses.

Compared with normal control brains, the brains of people with autism showed evidence of an ongoing inflammatory process in different regions of the brain and produced by cells known as microglia and astroglia, says Dr. Carlos Pardo

Logo courtesy of Johns Hopkins Medicine

Image courtesy of The University of Rochester


http://www.youtube.com/watch?v=Y9tBl4ATPZ0

http://www.urmc.rochester.edu/news/story/index.cfm?id=3019

http://www.hopkinsmedicine.org/Press_releases/2004/11_15a_04.html

Factors that can contribute to microglial activation in autism

Children with autism suffer from many chronic neurotropic infections that are associated with microglial activation.

Generally, chronic infections can last several days, months or a lifetime and at times, people do not know they are infected

What is a chronic infection?

The role of microglia in the infections of the central nervous system

These are agents that prefer to infect the central nervous system

What are neurotropical infectious agents?

Examples of neurotropic infections commonly present in children with autism

For additional information, you may read the medical article published by the investigators at the University of Minnesota – School of Medicine

Viruses ₋ Herpes Family Virues ₋ Coxsackie ₋ Measles ₋ Rubella ₋ Mumps

Bacteria ₋ Lyme Disease

Fungi Parasites

For additional details, click on the Minnesota University logo.


***Anything that can cause an abnormal immune reaction can activate the microglia

What can be done to regulate the microglial cells in autism?

Autism should be treated similarly to how the Nobel Prize-winning geneticist Dr. Mario Capecchi did. By treating the brain’s immune system (microglia).

2010 The Geneticist Winner of the Nobel Prize Dr. Mario Capecchi, “What we’re saying is microglia are much more sophisticated and are actually controlling behavior, and they have to do it by interacting the nerve cells in your brain," Capecchi says.

Photo Courtesy of Science News

According to scientists, during chronic microglial activation too many brain connections are destroyed. If the immune system is treated successfully, the synapses can be completely regenerated.

• Children with autism must be treated with medications that can block microglial activation.

• Also, they should be treated with medications that can keep viruses, bacteria, fungus and parasites under control.

• The Nobel Prize-winning geneticist Dr. Mario Capecchi says that instead of treating mental illness the traditional way that doctors do, with drugs to alter brain chemistry, he tried a new approach, by treating the immune system.


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• We have published and shared a breakthrough medical treatment that targets and block microglial activation which recent medical research is showing that it is at the center stage of all the common neurological and medical symptoms in autism.

• We have created a comprehensive medical protocol in English and Spanish that can be easily followed by doctors.

• There are over 50 medical doctors worldwide treating children with autism using the medical protocol that Juan Rodriguez discovered.

SCIA Protocol – English Edition SCIA Protocol – Spanish Edition


Our Medical Treatment Protocol For Autism

Note: The SCIA medical treatment protocol is intended to be followed under the medical supervision. Please read the Terms And Conditions in the SCIA website carefully before you proceed to download the SCIA Medical Treatment Protocol document and start the protocol.

https://www.stopcallingitautism.org/termsandconditions.html

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• Main part of treatment 1. Prescription strength Ibuprofen to

reduce microglial activation (10 days per month)

2. Specific daily of probiotics helps modulate the immune response and keep the stomach lining healthy

• Medications to treat infections • Viral • Fungus • Bacterial • Parasites

• Medications & supplements to support immune system function

• Diet


How does the SCIA protocol works?

Microglial Activation In Autism The #1 goal of the SCIA protocol is to block Microglial Activation

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• Selective serotonin reuptake inhibitors (SSRIs) are believed to act by inhibiting the reuptake of serotonin after being released in synapses.

• Anti psychotic (old) Haloperidol, Chlorpromazine, Thioridazine, and Fluphenazine. They work by controlling the intensity of the neurotransmitter dopamine in the brain.

• Anti psychotic (new) – Risperdal works in the brain, where it affects various neurotransmitters, in particular dopamine and serotonin

• Anticonvulsants work by blocking different neurotransmitters

• Stimulants – Ritalin significantly increases levels of dopamine in the brain, thereby stimulating attention and motivational circuits that enhance one's ability to focus and complete tasks.


5 Top Mainstream Drugs For Autism

Image courtesy of Carmen Gottfried and Rudimar Riesgo "Autism Spectrum Disorders - From Genes to Environment",

These drugs work by altering the brain chemistry ONLY addressing the symptoms and not the root cause!

These drugs attempt to treat the neurological symptoms

by altering the brain chemistry. They don’t target the root

cause which is microglial activation.

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• Hyperbaric Oxygen Therapy (HBOT) works by reducing nitric oxide levels generated when the microglial cells are activated. When the nitric oxide levels are reduced the astrocytes allow the blood vessels in the brain to expand increasing blood flow and oxygen in the brain. The problem is that the microglial cells are still activated causing many children to regress after the HBOT therapy is over.

• Chelation works by reducing the heavy metal toxicity and oxidative stress. Even after chelation children continue to suffer from heavy metal accumulation. Microglial cells generate glutathione (a powerful antioxidant), but when they become activated the glutathione levels are reduced not allowing the body to get rid off heavy metals effectively.


Example Of Current Biomedical Treatments For Autism

Nitric Oxide is a very important inflammatory mediator. In a low levels it is very healthy, but in elevated levels it is very harmful to the body

Several biomedical treatments show to be beneficial in the

treatment of autism. Many parents report improvements,

but also report regression after a period of time after the

therapies. This is caused because these treatments do not

block microglial activation directly.

Image Courtesy of “N. Allen and B. Barres, 2009”

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Juan Rodriguez have recently published a medical research article that

provides a very accurate and simple medical explanation for autism.


Our Medical Research Provides An Explanation For The Autism Puzzle

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• World renowned scientists agree with Stop Calling It

Autism! that the root cause of most mental illnesses

including autism is microglial activation

•Dr. Mario Capecchi, Ph.D, Winner Nobel Prize - Medicine

and geneticist at the University of Utah – he published a new study

establishing the cause and effect relationship between the immune

system, specifically microglial cells and psychiatric disorders.

R. Douglas Fields, Ph.D. - Chief of the Nervous System Development and

Plasticity Section, National Institute of Child Health and Development

(NICHD). Recently, he authored the book “The Other Brain”. Dr. Fields

states understanding the glia is the new frontier in brain science.

Carlos Pardo, MD - Associate Professor of Neurology and Pathology at

Johns Hopkins. His research focuses on immunological and molecular

mechanisms of neurological diseases. He also studies the mechanisms

involved in the neuroimmunological diseases linked to autism.

Dr. Mario Capecchi Photo courtesy of Science News

Dr. Douglas Fields Photo by Kay

Chernush - courtesy of NIH

Dr. Carlos Pardo Photo courtesy of

Science News


Bringing the future of brain science today

http://neuroscience.med.utah.edu/Faculty/Capecchi.html

http://neuroscience.med.utah.edu/Faculty/Capecchi.html

http://neuroscience.nih.gov/Lab.asp?Org_ID=274

http://www.neuro.jhmi.edu/neuroimmunopath/lab.htm



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• Cancer • In brain cancer microglial cells when activated

are who send the signals for the tumors to grow.

• Microglial activation leads to brain metastasis in breast cancer patients

• Alzheimer’s Disease • Parkinson’s Disease • Schizophrenia • Severe Depression • Down Syndrome • And more…


Is Microglial Activation Real?

http://www.siteman.wustl.edu/ContentPage.aspx?id=1834

http://cbcrp.org.127.seekdotnet.com/research/PageGrant.asp?grant_id=8474

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• We need to make treatments that block microglial activation available for individuals with autism that need them.

• We need a pharmaceutical company that can help us bring my discovery to your local pharmacy

• This breakthrough treatment can be available very soon if the right people get together

• If this treatment is prescribed to children as soon as they develop the initial autistic symptoms they could be completely cured in a very short time


Development of Treatments That Block Microglial Activation

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• Keep pregnant moms healthy and happy ( diet, exercise & stress free) • Take probiotics during pregnancy • Avoid taking any medications during pregnancy (*if possible) • Avoid C-Sections (*if possible) • Make sure that moms take probiotics while breastfeeding • Breastfeed as long as possible • Supplement infants with probiotics on a daily basis • Reduce the use of antibiotics in children • Limit the children’s exposure to anything that is known to activate the

microglia (viruses, bacteria, fungus, parasites, toxins, etc…)


Tips to Prevent Autism

In many children, autism is caused by an abnormal response in their brain’s immune systems (microglial activation). Children with healthier and better developed immune systems are less likely to develop autism.

Below is a list of things that are under our control that can greatly reduce the probability of a child from developing autism.

Probiotics and Toddler Health The immune system begins to develop from birth as the gut microflora becomes populated with a variety of microorganisms.

Photo courtesy of Nestlé

Visit www.stopcallingitautism.org for more details. Did you know that breast milk is rich in probiotics?

http://medical.gerber.com/clinicaltopics/articles.aspx?articleId=25EF3108-1620-434B-9D81-2ED7124E3D35&sec=articles&topicId=28bf6385-728a-4a60-b67c-f69baed67add

http://www.stopcallingitautism.org/

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• Modulate the immune response • Enhance the mucosal gut barrier function • Prevention and treatment of GI diseases • Prevention of atopic dermatitis • Reduced risk of food allergies


Importance of Probiotics

Probiotics are defined by the World Health Organization as live, non-pathogenic microorganisms that, when administered in adequate amounts, modify the intestinal microbial population in a way that confers a health benefit to the host. The benefits of probiotics as a supplement to safely and effectively promote good health are increasingly well documented.

Benefits of probiotics

Immunity and Probiotics Detailed report on the effects of the probiotics in the immune system.

Photo courtesy of Danone

Did you know that a healthy human being carry 10 times more good bacteria than human cells?

• Breast milk • Women’s vaginas (vaginal delivery is extremely

important for babies) • Food (i.e.. fermented dairy products) • Probiotic supplements

Where can probiotics be found?

http://www.danone.com/index.php?option=com_docman&task=doc_download&gid=46

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• Using the information that I provided today we can put and end to this devastating epidemic

• Now we know what causes autism • We can explain how children with autism are

recovering • We know how we can prevent autism • The field of glial research is the future of brain

science • Glial research is growing exponentially • We have a treatment that blocks microglial activation

available today, we need to partner with a pharmaceutical company to make it available as a prepackaged product at our local pharmacies

• Let’s do this for our children and for our society!


Let’s Put An End To The Autism Epidemic


cell behind autism.

www.glialtechnologies.com



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Questions?


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Juan-Rodriguez-SCIA-AutismOne-2013 (1)