Jeffrey S. Kieft Curriculum Vitae

Jeffrey S. Kieft, Ph.D. Associate Professor and Howard Hughes Medical Institute Early Career Scientist Dept. of Biochemistry and Molecular Genetics University of Colorado Denver School of Medicine Mail Stop 8101 (303) 724-3257 (w) 12801 East 17th Ave, Room L18-9110 (303) 724-3215 (f) Aurora, CO 80045 jeffrey.kieft@ucdenver.edu Education

1986 – 1990 Bachelor of Science in Chemistry, United States Military Academy, West Point, NY Research project: Synthesis of novel metal-ion complexes of thiosemicarbazone ligands; Advisor: Dr. John P. Scovill.

1993 – 1997 Doctor of Philosophy in Chemistry, University of California, Berkeley, CA

Dissertation: Structure and thermodynamics of a metal ion binding site in the RNA major groove: Cobalt (III) hexammine as a probe; Advisor: Dr. Ignacio Tinoco, Jr.

1998 – 2001 Postdoctoral Associate, Dept. of Molecular Biophysics and Biochemistry and Howard Hughes Medical Institute, Yale University, New Haven, CT Research topic: Structure and function of the internal ribosome entry site RNA of the hepatitis C virus; Advisor: Dr. Jennifer A. Doudna.

Academic appointments

2002 – 2008 Assistant Professor, Dept. of Biochemistry and Molecular Genetics University of Colorado Denver School of Medicine, Aurora, Colorado

2008 - 2011 Director of Student and Faculty Affairs, Program in Structural Biology and Biophysics University of Colorado Denver School of Medicine, Aurora, Colorado

2008 – present Associate Professor (with tenure), Dept. of Biochemistry and Molecular Genetics University of Colorado Denver School of Medicine, Aurora, Colorado

2009 – present Early Career Scientist, Howard Hughes Medical Institute 2012 – present Visiting Scientist, HHMI/Janelia Farm Research Campus, VA Hospital, government or other professional positions

1990 – 1993 Active duty officer, US Army, Germany Positions: Tank Platoon Leader, Support Platoon Leader, Battalion Logistics Officer, Honorable Discharge from Active duty

1993 – 1997 Reserve officer, US Army, Dublin, CA

Positions: Observer/Controller, Brigade Headquarters and Headquarters Company Commander

2001 – 2002 AAAS/Roger Revelle Fellow, White House Office of Science and Technology Policy, Executive Office of the President, Washington, DC

Kieft, Jeffrey S.

12/6/2013

Honors, special recognitions, and awards

1987 – 1988 Distinguished Cadet, West Point 1988 Exchange student to United States Naval Academy (6 chosen from 900+ cadets) 1990 Daughters of the American Revolution Award, West Point Class of 1990 (Given to

graduate ranked highest in required English classes) 1990 Phi Kappa Phi 1990 – 1993 Army commendation medal (x2), National Defense Service Medal, Army Service

Ribbon, Overseas Service Ribbon, Parachutists Badge, Air Assault Badge 1997 Nominee to NASA Astronaut Program by US Army 1999 Yale Nominee for Burroughs-Wellcome Award 2001 – 2002 AAAS/Roger Revelle Fellow in Global Stewardship 2002 – 2003 University of Colorado Howard Hughes Faculty Award 2003 UCHSC Nominee for Searle Scholar Award 2003 UCHSC Nominee for PEW Award 2003 UCHSC Nominee for V-Foundation Award 2004 – 2006 March of Dimes Basil O’Conner Scholar Award 2004 UCHSC Nominee for PEW Award 2008 University of Colorado School of Medicine Student Mentoring Award 2009 Early Career Scientist Award, Howard Hughes Medical Institute 2013 Univ. of Colorado School of Medicine Nominee for a Keck Foundation Award Grant support

Active – as Principle Investigator

4/12-3/16 NIH R01 GM097333-02 (PI: Kieft) NIH/NIGMS

Manipulation of cellular translation machinery by viral RNAs 9/08-7/17 NIH R01 GM081346-05 (PI: Kieft)

NIH / NIGMS Structural basis of protein synthesis by the hepatitis C virus IRES RNA

9/09–8/15 Howard Hughes Medical Institute Early Career Scientist Award (PI: Kieft)

Howard Hughes Medical Institute

Active – as Collaborator or Co-PI

9/12-8/13 Visiting Scientist Project (Co-PIs: Kieft, Gonen) HHMI / Janelia Farm Research Campus Ribosomes and localized translation at the synapse

4/11–3/16 NIH P01CA104106 (PI: Paschal) NIH / NCI

Signaling and Progression in Prostate Cancer $1,490,655 total direct for project 1 Role: Co-PI on project 1.

Completed – as Principle Investigator

2003-2004 HHMI Faculty Research Award (PI: Kieft) Dynamic Assembly of Hepatitis C Virus Translation Preinitiation Complexes

Kieft, Jeffrey S.

12/6/2013

2/04-2/05 American Cancer Society Seed Grant (PI: Kieft) Gene regulation at the level of translation: The role of proteins in IRES structure

7/03-7/05 Cancer League of Colorado (PI: Kieft) RNA structure-dependent regulation of a virus associated with liver cancer 2/04–1/06 March of Dimes Basil O’Conner Research Scholar Award (PI: Kieft)

Understanding the Role of IRES RNAs in Preventing Aberrant Protein Expression and Resultant Birth Defects

3/06 UCCC/MBC Opportunity Award (PI: Kieft) Structural studies of antitumor DNAzymes

2/06-2/07 American Cancer Society Seed Grant (PI: Kieft)

Structure and function of RNAs from cancer-causing viruses

2/07-2/08 UCCC Seed Grant (PI: Kieft) Structural and biochemical studies of an RNA that inhibits RNAse L, an enzyme

associated with prostate cancer 10/07 S10 RR024632 Shared Equipment Grant (PI: Kieft)

NIH /NCRR Automated Macromolecular Crystallization System funds

2/08–8/08 American Cancer Society Research Scholar Award (PI: Kieft) Structural basis of protein synthesis by a cancer-causing virus (Note: grant was terminated 8/31/08 due to overlap with awarded R01)

7/07–6/09 NIH R03 AI072187 (PI: Kieft)

NIH/NIAID Development of tools for structural genomics of viral RNA 3/05-2/11 NIH R01 GM072560 (PI: Kieft)

NIH/NIGMS Structural and biophysical analysis of a viral IRES

Completed – as Collaborator or Co-PI

2003-2004 Butcher Award (Co-PIs: Kieft & Batey) Development of Novel Purification Schemes for RNA Structural Studies

5/06- 2/07 University of Colorado Technology Transfer Award (Co-PIs: Kieft & Batey) RNA Purification by nondenaturing affinity chromatography. 9/10-8/14 NIH R01 HL075646 (PI: Dr. Annabelle Rodriguez, Johns Hopkins)

NIH / NHLBI The role of Scavenger Receptor Class B Type I in humans Role: Collaborator

Kieft, Jeffrey S.

12/6/2013

Bibliography

Peer-reviewed primary research articles 1. Kieft, J.S., & Tinoco, I. Jr. (1997). Solution structure of a metal-binding site in the major groove of RNA

complexed with cobalt (III) hexammine. Structure 5, 713-721. 2. Kieft, J.S., Zhou, K., Jubin, R., Murray, M.G., Lau, J.Y.N. & Doudna, J.A. (1999). The hepatitis C virus

internal ribosome entry site adopts an ion-dependent tertiary fold. J. Mol. Biol. 292, 513-529. 3. Jubin, R., Vantuno, N.E., Kieft, J.S., Murray, M.G., Doudna, J.A., Lau, J.Y.N. & Baroudy, B.M. (2000).

Hepatitis C virus internal ribosome entry site loop IIId contains a phylogenetically conserved GGG triplet essential for translation initiation and IRES folding. J. Virol. 74, 10430-10437

4. Kieft, J.S. Zhou, K. Jubin, R. & Doudna, J.S. (2001). Mechanism of ribosome recruitment by hepatitis C IRES RNA. RNA 7, 194-206.

5. Rijnbrand, R., Bredenbeck, P.J., Haasnoot, P.C., Kieft, J.S., Spaan, W.J.M. & Lemon, S.M. (2001). The influence of downstream protein-coding sequence on internal ribosome entry on hepatitis C virus and other flavivirus RNAs. RNA 7, 585-597.

6. *Spahn, C.M.T., *Kieft, J.S., Grassucci, R.A., Pencek, P., Zhou, K., Doudna, J.A. & Frank, J. (2001). Hepatitis C virus IRES RNA induced changes in the conformation of the 40S subunit. Science 291, 1959-1962. *These authors contributed equally to this work

7. Kieft, J.S., Zhou, K., Grech, A., Jubin, R., & Doudna, J.A. (2002) Crystal structure of a four-way RNA junction from the HCV IRES, Nat. Struct. Biol. 9, 370-374.

8. *Kieft, J.S. & *Batey, R.T. (2004) A general method for rapid and nondenaturing purification of RNAs. RNA, 10, 995-998. *These authors contributed equally to this work

9. Costantino, D. & Kieft, J.S. (2005) A pre-formed compact ribosome-binding domain in the cricket paralysis-like virus IRES RNAs, RNA 11, 332-343.

10. Pfingsten, J.S., Costantino, D.A & Kieft, J.S. (2006) Structural basis for cap-independent ribosome recruitment and manipulation by a viral IRES. Science, 314, 1450-1454.

11. Pfingsten, J.S., Costantino, D.A & Kieft, J.S. (2007) Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes. J. Mol. Biol., 370, 356-369.

12. *Batey, R.T. & *Kieft, J.S. Improved native affinity purification of RNA (2007) RNA, 13, 1384-1389. *These authors contributed equally to this work

13. Keel, A.Y., Rambo. R.P., Batey, R.T., & Kieft, J.S. (2007) A general method to solve the phase problem in RNA crystallography. Structure 15, 761-772.

14. Costantino, D.A., Pfingsten, J.S., Rambo, R.P., & Kieft. J.S. (2008) tRNA-mRNA mimicry drives translation initiation from a viral IRES. Nat. Struct. Mol. Biol. 15, 57-64.

15. Timmerman, S.L., Pfingsten, J.S., Kieft, J.S., & Krushel, L.A. (2008) The 5' Leader of the mRNA Encoding the Mouse Neurotrophin Receptor TrkB Contains Two Internal Ribosomal Entry Sites that Are Differentially Regulated. PLoS ONE. 3, e3242.

16. Hammond, J.A., Rambo, R.P., Filbin, M.E., & Kieft, J.S. (2009) Comparison and functional implications of the 3-D architectures of viral tRNA-like structures. RNA, 15, 294-307.

17. Lee S.J., Hyun S., Kieft J.S., & Yu J. (2009) An approach to the construction of tailor-made amphiphilic peptides that strongly and selectively bind to hairpin RNA targets. J. Am. Chem. Soc. 131, 2224-2230.

18. Liu W., Zhao R. McFarland C., Kieft J.S., Niedzwiecka A., Jankowska-Anyszka M., Stepinski J., Darzynkiewicz E., Jones D.N., & Davis R.E. (2009) Structural insights into parasite eIF4E binding specificity for m7G and m2,2,7G mRNA caps. J. Biol. Chem. 6:31336-31349.

19. Pfingsten, J.S., Castile, A.E., & Kieft, J.S. (2010) Mechanistic role of structurally dynamic regions in the Dicistroviridae IGR IRESs. J. Mol. Biol., 395, 205-217.

20. Constantineau J., Greason E., West M., Filbin M., Kieft J.S., Carletti M.Z., Christenson L.K. & Rodriguez A. (2010) A synonymous variant in scavenger receptor, class B, type I gene is associated with lower SR-BI protein expression and function. Atherosclerosis 210, 1771-1782.

21. Hammond, J.A., Rambo, R.P., & Kieft, J.S. (2010) Multi-domain packing in the aminoacylatable 3’ end of a plant viral RNA. J. Mol. Biol.,399, 450-463.

22. Kieft, J.S., Chase, E., Costantino, D.A., & Golden, B.L. (2010) Identification and characterization of anion binding sites in RNA. RNA, 16, 1118-1123.

Kieft, Jeffrey S.

12/6/2013

23. Feser, J., Truong, D., Das, C., Carson, J.J., Kieft J.S., Harkness, T., & Tyler, J.K. (2010) Elevated histone expression promotes life span extension. Mol. Cell, 39, 724-735.

24. Zhu, J., Korostelev, A., Costantino, D.A., Donohue, J.P., Noller, H.F., & Kieft, J.S. (2011) Crystal structures of complexes containing domains from two viral internal ribosome entry site (IRES) RNAs bound to the 70S ribosome. Proc. Natl. Acad. Sci. U.S.A. 108, 1839-1844.

25. Redzic J.S., Armstrong G.S., Isern N.G., Jones D.N., Kieft J.S., & Eisenmesser E.Z. (2011). The retinal specific CD147 Ig0 domain: from molecular structure to biological activity. J Mol Biol 411: 68-82.

26. Vallejos M., Deforges J., Plank T-D., Letelier A., Ramdohr P., Abraham C.G., Valiente-Echeverria F., Kieft J.S., Sargueil B., &Lopez-Lastra M. (2011). Activity of the human immunodeficiency virus type 1 cell cycle-dependent internal ribosomal entry site is modulated by IRES trans-acting factors. Nucleic Acids Res 39, 6186-6200.

27. Filbin, M.E. & Kieft, J.S. (2011) HCV IRES domain IIb affects the configuration of coding RNA in the 40S subunit's decoding groove. RNA 17, 1258-1273.

28. Liu, W., Jankowska-Anyszka, M., Piecyk, K., Dickson, L., Wallace, A., Niedzwiecka, A., Stepinski, J., Stolarski, R., Darzynkiewicz, E., Kieft J., Zhao R., Jones D.N.,& Davis R.E. (2011) Structural basis for nematode eIF4E binding an m(2,2,7)G-Cap and its implications for translation initiation. Nucleic Acids Res. 39, 8820-8832.

29. Keel, A.Y., Jha, B.K., & Kieft, J.S. (2012) Structural architecture of an RNA that competitively inhibits RNase L. RNA 18, 88-99.

30. Filbin, M.E. Vollmar, B. S., Shi, D., Gonen, T., & Kieft, J.S. (2013) HCV IRES manipulates the ribosome to promote the switch from translation to elongation. Nat Struct Mol Biol 20, 150-158.

31. Wang, H., Ru, Y., Sanchez-Carbayo, M., Wang, X., Kieft, J.S., & Theodorescu, D. (2013) Translation initiation factor eIF3b expression in human cancer and its role in tumor growth and lung colonization. Clin Cancer Res. 19, 2850-2860.

32. Plank, T-D.M., Whitehurst, J.T., & Kieft, J.S. (2013) Cell type specificity and structural determinants of IRES activity from the 5’ leaders of different HIV-1 transcripts. Nucleic Acids Res. 41, 6698-6714.

Peer reviewed book chapters and reviews

1. Invited review: Kieft, J.S. (2008) Viral IRES RNA structures and ribosome interactions. TiBS, 33, 274-283.

2. Invited review: Pfingsten, J.S. & Kieft, J.S. (2008) RNA structure-based translation initiation: Lessons from the Dicistroviridae intergenic region IRESs. RNA, 14, 1255-63.

3. Invited review: Kieft, J.S. (2009) Comparing the three-dimensional structures of Dicistroviridae IGR IRES RNAs with other viral RNA structures. Virus Res. 139, 148-56. (Invited Co-editor for this special edition: “Structural Motifs controlling the replication cycle of positive strand RNA viruses”)

4. Invited review: Filbin, M.E. & Kieft, J.S. (2009) Towards a structural understanding of IRES RNA function. Curr Opin Struct Biol 19, 267-276.

5. Invited review: Plank, T-D. & Kieft, J.S. (2012) The structures of nonprotein-coding RNAs that drive internal ribosome entry site function. Wiley Interdiscip Rev RNA. 3, 195-212.

Non-peer reviewed book chapters, reviews, etc.

1. Tinoco, I. Jr. & Kieft, J.S. (1997). The ion core in RNA folding (News and Views). Nat. Struct. Biol. 4, 509-512.

2. Kieft, J.S., Grech, A., Adams, P. & Doudna, J.A. (2001). Mechanisms of internal ribosome entry in translation initiation. Cold Spring Harb Symp Quant Biol. 66, 277-283.

3. Kieft, J.S. & Pfingsten, J. (2005) Weapons in the molecular arms race (News and Views). Nat. Struct. Mol. Biol. 12, 938-939.

4. Kieft, J.S., Costantino, D.A., Filbin, M.E., Hammond J., & Pfingsten, J.S. (2007) Structural methods for studying IRES function. Methods Enzymol., 430, 333-371.

5. Kieft, J.S. Biophysical analysis of IRES RNAs from the Dicistroviridae: linking architecture to function, in Non-protein coding RNAs; Springer Series in Biophysics Vol 13., N. Walter, S.Woodson, and R. Batey, Eds. 2008, Springer Berlin, Heidelberg, p 317-333.

Kieft, Jeffrey S.

12/6/2013

6. Keel, A.Y. Easton, L.E., Lukavsky, P.J., & Kieft, J.S. (2009) Large-scale native preparation of in vitro transcribed RNA, Methods Enzymol, 469, 3-25.

Other articles

12/3/2006 Lead Op-Ed in the Perspective section of the Denver Post: Standing up for Science; Evolution Key to Biology

2/2012 Perspective/interview. HHMI Bulletin: Inspired to serve. Easing the conflict between faith and evolution.

Other products of scholarship

Invited Lecturer for a CD-ROM entitled “Understanding Ribosomes” produced by Henry Stewart Talks, London, England (editor: Tom Steitz). Titles of lectures “Translation initiation and the structures of eukaryotic initiation factors,” and “Factor independent initiation of protein synthesis by IRES RNAs.” 2008 Contributed articles entitled “Eukaryote,” “Nucleus,” and “Ribosome” to the World Book

Encyclopedia. Lectures and presentations, Local/Regional 2002 Invited speaker: University of Colorado, Boulder, Boulder, CO (Dept. of Chem. and

Biochem.) 2002 Speaker: Molecular Biology Graduate Program Seminar Series 2002 Invited speaker: University of Denver, Denver, CO (Dept. of Chem. and Biochem.) 2002-present Speaker: Several informal discussions and seminars in local “Translation Club” and related 2003 Speaker: Informal presentation of students about the interface of science and policy

(experiences from my time in the White House Office of Science and Technology Policy) 2003 Speaker: “RNA Club”, Univ. of Colorado, Boulder 2003 Butcher Awardee speaker: Butcher Symposium on Genomic and Biotechnology,

Broomfield, CO 2003 -present Speaker: Molecular Biology Program Annual Retreat (speak most years) 2005 Speaker: Microbiology Program weekly seminar series, Aurora, CO 2005 Speaker: MSTP student lunchtime seminar series 2006 Speaker: University of Colorado Cancer Center (UCCC) weekly seminar series 2006 Invited keynote speaker: The 2006 National meeting of the Presbyterian Association on

Science, Technology, and the Christian Faith (PASTCF) Topic: How Evolutionary Theory is used in modern scientific research, Denver, CO

2007 Speaker: Biomolecular Structure Program weekly seminar series 2007 Speaker: University of Colorado Cancer Center (UCCC) weekly seminar series 2007 Speaker: “RNA Club”, Univ. of Colorado, Boulder 2007 Speaker: MSTP student lunchtime seminar series 2008 Invited speaker: American Cancer Society Relay for Life in Durango, Colorado 2008 Speaker: Pharmacology Program weekly seminar series, Aurora, CO 2009 Speaker: University of Colorado Cancer Center (UCCC) weekly seminar series 2009 Speaker: “RNA Club”, Univ. of Colorado, Boulder 2009 Speaker: Rocky Mountain Virology Club Annual Meeting, Pingree Park, CO 2010 Invited speaker: Chemistry Dept., Colorado State University, For Collins, CO 2010 Speaker: Structural Biology and Biophysics weekly seminar series 2011 Invited Speaker: Chemistry Dept. Seminar, University of Denver, Denver CO 2011 Speaker: University of Colorado Cancer Center (UCCC) weekly seminar series 2011 Invited Speaker: University of Colorado Infectious Disease Symposium, Denver, CO

Kieft, Jeffrey S.

12/6/2013

2011 Speaker: Rocky Mountain Virology Club Annual Meeting, Pingree Park, CO 2013 Invited Speaker: Evolution of Proteins Meeting, UC Denver SOM, Aurora, CO 2013 Invited Speaker: Fort Lewis College, Durango, CO National 2000 Selected speaker: RNA Structure Meeting, Santa Cruz, CA, Banff, Canada 2001 Invited job seminar: University of Utah, Salt Lake City, UT (Dept. of Biochem.) 2001 Invited job seminar: University of California, Davis, Davis, CA (Dept. of Molecular and

Cellular Biology.) 2001 Selected speaker: Sixth Annual Meeting of the RNA Society, Banff, Canada 2001 Invited job seminar: Montana State University, Bozeman, MT (Dept. of Chem. and

Biochem.) 2001 Invited job seminar: University of Texas, Austin, TX (Dept. of Chem. and Biochem. and

Institute for Cellular and Molecular Biology) 2003 Invited speaker and panelist: American Association for the Advancement of Science

annual meeting, Denver, CO 2004 Invited speaker and panelist: American Association for the Advancement of Science

Fellowship recruitment event, Sandia National Labs, NM 2004 Invited speaker: Montana State University, Bozeman, MT (Dept. of Chem. and Biochem.) 2004 Selected speaker: Ninth Annual Meeting of the RNA Society, Madison, WI 2004 Invited speaker and panelist: American Association for the Advancement of Science

annual meeting, Seattle, WA 2006 Selected speaker: Cold Spring Harbor Translational Control Meeting, Cold Spring Harbor,

NY 2007 Invited speaker: Rockefeller University, New York, NY (Center for the Study of Hepatitis C) 2007 Invited speaker: University of Southern California School of Medicine, Pasadena, CA 2007 Invited speaker: National Meeting of the American Crystallographic Association, Salt Lake

City, UT 2007 Invited speaker: University of California, Irvine, CA (Center for Virus Research and PREP) 2008 Invited speaker: Wake Forest University, Winston-Salem, NC 2008 Invited speaker: Purdue University, West Lafayette, IN 2008 Invited speaker: University of North Carolina at Chapel Hill, NC 2008 Invited speaker: National Meeting of American Crystallographic Association, Knoxville, TN 2008 Invited speaker: Nucleic Acid Gordon Conference, Salve Regina University, RI 2008 Invited Speaker: American Chemical Society Midwest Region, Annual Meeting, Kearney,

NE 2008 Invited Speaker: University of California, Santa Cruz, CA. 2008 Selected speaker: Cold Spring Harbor Translational Control Meeting, Cold Spring Harbor,

NY 2009 Invited Speaker: University of Oregon, Eugene, OR 2009 Invited Speaker: 2009 Symposium on RNA Biology VIII, Wake Forest University, NC 2009 Speaker: Howard Hughes Medical Institute Early Career Scientist Meeting, Janelia Farm

Research Campus, VA 2010 Invited Speaker: Cornell University Biophysics Colloquia, Ithaca, NY 2010 Invited Speaker: Annual Meeting of the American Society for Biochemistry and Molecular

Biology, Anaheim California 2010 Invited Speaker: Iowa State University, Ames, Iowa 2010 Invited Speaker: “Tinoco Symposium,” University of California, Berkeley 2010 Invited Speaker: American Chemical Society Regional Meeting, New Orleans, LA 2010 Invited Speaker: Minority Access to Research Careers (MARC) Program, University of

Arizona, Tucson, AZ. 2011 Invited Speaker: University of Texas, Austin 2011 Invited Speaker: University of Wisconsin, Madison, WI

Kieft, Jeffrey S.

12/6/2013

2011 Invited Speaker: University of Washington, Seattle, WA 2011 Speaker: Howard Hughes Medical Institute Science Meeting, Janelia Farm Research

Campus, VA 2012 Invited Speaker: FASEB meeting on Catalytic RNAs, Snowmass, CO 2013 Invited Speaker: “Food for thought” presentation at HHMI Headquarters (on behalf of Janelia

Farm Visitor Program) 2013 Speaker: Howard Hughes Medical Institute Science Meeting, HHMI Headquarters, Chevy

Chase, MD 2013 Invited Speaker: Zing Ribosome meeting, Napa, CA 2013 Invited Speaker: Third International Conference on Nucleic Acid-Protein Chemistry and

Structural Biology, Georgia State University, Atlanta, GA International 2005 Invited speaker: European Molecular Biology Laboratory (EMBL), Heidelberg, Germany 2006 Invited speaker: European Molecular Biology Laboratory (EMBL), Grenoble, France 2007 Invited Plenary and Symposium Speaker, ComBio2007; The annual meeting of the

Australian Society for Biochemistry and Molecular Biology, Sydney, Australia 2008 Invited speaker: International Conference on the Crystallization of Biological

Macromolecules, Cancun, Mexico 2008 Invited speaker: The XXI Congress and General Assembly of the International Union of

Crystallography, Osaka, Japan (Declined) 2010 Selected Speaker: Zing Conference on Nucleic Acids, Cancun, Mexico 2011 Selected Speaker: EMBL Conference on Protein Synthesis and Translational Control,

Heidleberg, Germany Membership in professional organizations

2001 – present American Association for the Advancement of Science 2002 – present RNA Society 2002 – present University of Colorado Cancer Center Committee and service responsibilities

Departmental, School of Medicine, and University

2002 University of Colorado Homeland Security Task Force 2003 – present X-ray Crystallography Shared Resource (Core Facility) Steering Committee 2006 – 2007 Department Representative, Faculty Senate 2003 – 2007 Chairman, Seminar Committee, Molecular Biology Program 2004 Department of Biochemistry and Molecular Genetics Faculty Search Committee 2004 Biomolecular Structure Faculty Search Committee 2004 Exhibitor on behalf of Structural Biology at the Research Tower Grand Opening on

the Fitzsimons Campus 2004 – 2006 Medical Scientist Training Program (MSTP) Admissions Committee 2007 – 2011 Molecular Biology Graduate Program Steering Committee 2009 Chair, Department Medical School Curriculum Review Committee 2009 – present Chair, Department Medical School Teaching Committee 2010 Faculty Advisor: 25th Annual National MD/PhD Student Conference in Keystone, CO 2010 – 2011 Chair, Department of Biochemistry and Molecular Genetics Faculty Search

Committee 2008 – present Medical Scientist Training Program (MSTP) Steering Committee 2011 Molecular Biology Graduate Program Preliminary Exam Format Review Committee 2011 – present Chair, Structural Biology and Biochemistry Graduate Program Preliminary Exam

Committee

Kieft, Jeffrey S.

12/6/2013

2012 – present Structural Biology and Biochemistry Graduate Program Training Committee 2013 – present Department of Biochemistry and Molecular Genetics Postdoc Recruitment Committee 2013 – present Department Reorganization Mission Statement Committee

National and international

2002 Chairman, National Science and Technology Council Research Performance Criteria Working Group

2002 Co-Chairman, National Science and Technology Council Committee on Environment and Natural Resources, National Hypoxia Assessment Working Group

2002 Chairman, National Science and Technology Council Committee on Environment and Natural Resources, Ocean Observing System Working Group

2002 Member, Mississippi River/Gulf of Mexico Watershed Nutrient Task Force 2002 White House Office of Science and Technology Policy (OSTP) Representative,

National Ocean Partnership Program Interagency Working Group 2002 White House Office of Science and Technology Policy (OSTP) and Office of

Homeland Security (OHS) Representative, Container Security Working Group, Technology Sub-Group

2010 - present Faculty Member: “Faculty of 1000” in the area of “Structure: RNA” 2013 Ad-hoc Member of the American Cancer Society Council for Extramural Grants 2014 (upcoming) Organizer of the 2014 FASEB meeting: “Machines on Genes”, Snowmass, CO

Other community service

1996 – 1997 Volunteer, Boston Museum of Science: Science by Mail Program 2005 – 2011 Board of directors, Self-Improvement Opportunities, a local nonprofit in Aurora, CO 2006 – present Cofounder of the Colorado Evolution Response Team (CERT) 3/2007 Invited Adult Education Instructor of a four-week series at Montview Boulevard

Presbyterian Church, Denver, Topic: Evolution: Right, wrong, revelation, or revolution?

6/2007 Keynote Speaker at the 2006 National meeting of the Presbyterian Association on Science, Technology, and the Christian Faith (PASTCF) Topic: Evolutionary Theory

4/2008 Invited Adult Education Instructor of a four-week series at Shepherd of the Hills Presbyterian Church, Lakewood, CO, Topic: Evolution: Right, wrong, revelation, or revolution?

6/2008 Invited Researcher Guest Speaker: American Cancer Society Relay for Life, Durango, Colorado

2/2009 Invited speaker: Max Pickerell Lecturer, Colby Community College, Colby Kansas. Topic: “Teaching evolutionary theory: why it matters”

3/2009 Invited speaker at “Darwin Days” Celebration, Northwest Missouri State University. Topic: “Evolutionary theory: A critical tool to understand biology and improve human lives”

11/2009 Invited Speaker at Grand Junction Presbyterian Church on the topic of evolutionary theory and religion

2009 – present Scientific Advisor for the Clergy Letter Project 5/2011 Noontime speaker: Anschutz Medical Campus Library in support of Darwin Program:

“Religion and evolution: why they still haven’t reconciled” 10/2011 Speaker (with Prof. James Degregori) at Café Scientific II, a forum for scientific

discussions with the public. Title: “Evolution: It’s not just for geeks anymore” 2012 – present Volunteer and presenter at local schools and the DU Chamberlin Observatory on

behalf of the Denver Astronomical Society.

Kieft, Jeffrey S.

12/6/2013

Inventions, intellectual property and patents held or pending US Patent # 8,084,399: Yu; Jaehoon (Seoul, KR), Kieft; Jeffrey (Denver, CO), Lee; Su Jin (Seoul, KR)

“Process for screening of a binding amphiphilic peptides specific for hairpin RNA” Review and referee work Editorial boards 2013 – present Editorial Board member of the journal “Translation” Grant review committees and study sections 2006 - present Beamtime proposal reviewer: Advanced Photon Source (APS), Argonne National

Lab 2007 Ad-hoc reviewer: Welcome trust Programme Grant reviewer 2007 Ad-hoc reviewer: FWF Austrian Science Fund 2008 - 2012 Member: American Cancer Society, RNA Mechanisms in Cancer Review Panel 2012 Chair: American Cancer Society, RNA Mechanisms in Cancer Review Panel 2009 Ad-hoc Reviewer: Qatar National Research Fund 2009 - present Ad-hoc Reviewer, National Science Foundation 2010 University of Colorado School of Medicine PEW Scholar review board 2011 Ad-hoc Reviewer, National Institutes of Health Special Panel 2012 Ad-hoc Reviewer, US-Israel Binational Science Foundation 2012 Pre-screen reviewer: HHMI International Student Research Fellowships program 2013 Ad-hoc Member of the National Cancer Society Council for Extramural Grants 2013 Ad-hoc Reviewer, National Institutes of Health Special Panel 2013 Ad-hoc Reviewer, National Institutes of Health Molecular Structure and Function–C

(MSFC) Study Section Reviewer for journals, professional societies or scientific meetings 2002 – present Manuscript reviewer for the journals:

Science, Nature, Cell, Nature Structural and Molecular Biology, Journal of Molecular Biology, EMBO J, EMBO Reports, Nucleic Acid Research, RNA, Journal of Virology, Nature Reviews, Biochemistry, Proceeding of the National Academy of Sciences, Virus Research, Genes and Development, Virus Research, Journal of Biophysical Chemistry, Structure, Wiley Interdisciplinary Reviews, Translation, etc.

2006, 2007 Application Reviewer, AAAS Science Policy Fellowship Program 2007 Butcher Symposium Session Chair, Broomfield, CO 2009 “Viral RNAs” Session Chair at the Annual Meeting of the RNA Society 2012 “Regulatory Elements” Session Chair at the Translational Control Meeting at Cold

Spring Harbor, NY Teaching record

Medical students

IDPT 6002 Digestive, Endocrine, and Metabolic Systems (previously BMGN 5001, Metabolic Biochemistry): 2004, 2005, 2006, 2007, 2008

IDPT 5002 Molecules to Medicine 2007, 2008, 2009, 2010, 2011, 2012

Kieft, Jeffrey S.

12/6/2013

Graduate students

MOLB 7801 Molecular Biology Advanced Topics 2003, 2004, 2005, 2006, 2010, 2013

IDPT 7811 Biomedical Science Core Course 2003, 2004, 2005, 2006, 2007, 2008, 2009, 2010 (course director), 2011, 2012 (course

director) MICB 7627 Molecular Virology and Pathogenesis 2004, 2005, 2006, 2007, 2008, 2009, 2010 BMST 7454 Structural Analysis of Biomolecules II 2006, 2008, 2010, 2012 BMST 7350 Proteins 2013 2006, 2007 Guest lecture at University of Colorado, Denver: One hour lecture on X-ray crystallography 2002-present Leader of various paper discussion sections (Biomedical Sciences Program, Molecular

Biology Program, MSTP, etc.) Undergraduates 2010 Invited Speaker: Minority Access to Research Careers (MARC) Program, University of

Arizona, Tucson, AZ. 2013 Invited Speaker: Fort Lewis College, Durango, CO. Course directing IDPT 7811 Biomedical Science Core Course, first block 2010, 2012

Graduate program directing or advising

2008 – 2011 Director of Student and Faculty Affairs, Program in Structural Biology and Biophysics 2007 – 2011 Molecular Biology Graduate Program Student Advisor 2012 – present Structural Biology and Biochemistry Graduate Program Student Advisor

Graduate Program Affiliations

Molecular Biology Program, Program in Structural Biology and Biochemistry, Biomedical Sciences Program, Medical Scientist Training Program (MD/PhD), Microbiology Program, Pharmacology Program Mentoring and training

Ph.D. Students and Postdoctoral Fellows Trained

2002 – 2007 Jennifer Pfingsten (student: Biochemistry Ph.D. Program) (Currently Senior Scientist at Somalogic, Boulder, CO)

2002 – 2008 John Hammond (student: Molecular Biology Ph.D. Program) (Currently post-doc in laboratory of Jamie Williamson, Scripps) 2004 – 2010 Amanda Keel (student: Biochemistry Ph.D. Program)

(Completed post-doc with Nicholas Seeds, currently taking time off for health reasons)

2006 – 2011 Megan Filbin (student: Molecular Biology Ph.D. Program) (Currently visiting post-doctoral scientist at HHMI/Janelia Farm Research Campus, VA)

2009 –2012 James Whitehurst (student: MD/PhD Program and Pharmacology Program) (Currently completing medical school)

Kieft, Jeffrey S.

12/6/2013

2008 – present Terra-Dawn Plank (student: Molecular Biology Ph.D. Program) (Will begin post-doc in lab of Miles Wilkinson, UC San Diego in Winter 2014) 2009 – present Yumeng Hao (student: Molecular Biology Ph.D. Program) 2009 – present Marisa Ruehle (student: Molecular Biology Ph.D. Program) 2009 – present Timothy Colussi (Post-Doctoral Fellow from Wake Forest) 2011 – present Erich Chapman (Post-Doctoral Fellow from Univ. of Oregon) 2011 – present Zane Jaafar (student: Microbiology Ph.D. Program) 2013 – present Erik Hartwick (student: Structural Biology and Biochemistry Ph.D. Program) Rotation students: Courtney Hollins, Erica McCoy, Michael Guarnieri, Clifton Hackbarth, Iona Bartek,

Chris Washenberger, Alice Castile, Kris Brannan, Jasmina Redzic, Christopher Abraham, Sam Li, Sarah Martin, Vishantie Dostal, Ryan Sheridan

Thesis and comprehensive examination committees

Thesis committees: Stephanie Timmerman (graduated), Christine English (graduated), Brooke Bishop (chair, graduated), Wai-Lun (Patrick) Li (graduated),David Zoeteway (graduated), Dan Felmlee (graduated), Benjamin Steil (graduated), Aaron Patrick (chair, graduated), Brigid Bucci (graduated), Anna Thode (graduated), John Laughlin (graduated), Todd Ganglehoff (graduated), Michael Guarnieri (chair, graduated), Greg Caporaso (graduated), Clifton Hackbarth (graduated), Kevin Diebel (graduated), Bethany Veo (graduated), Sten Wie (chair), John Jiang (graduated), Tara Dobson (graduated), Alice Castile (chair, graduated), Jamie Collier (chair, graduated), Jasmina Redzic (chair, graduated), Brandi Chong, Mike Holliday, Aga Kendrick (chair), Christina Wysoczynski, Wallace Liu, Jovylyn Gatchalian, Katherine Shives

Additional comprehensive examination committees: Jamie Kean, Douglas Donham II, Linda Akerman, Richard Sullivan

Other Selected Student Mentoring Activities

2007 Alternative Careers in Science Club presenter on science policy careers 2010 Breakout session discussion leader at 25th annual National MD/PhD Student

Conference in Keystone, CO. Topic: how to write a paper. 2011 Member of career panel – Pharmacology Dept. Retreat 2011 Member of career panel – Molecular Biology Program Round Table 2011 Ethics discussion leader for incoming graduate students 2013 Member of career panel – Academic job search, what to expect Consulting

1997 Maxigen, Inc. Assisted in molecular dynamics simulations for a paper published in Nature [Crameri et al. (1998) Nature 391, 288-291].

2009 Consultant of Reglera Corporation in effort to obtain FDA approval for medical device 2012 Consultant for Capital Consulting: “Protein Crystallization on the International Space Station”